Spectrophotometric determination of bifonazole in pharmaceutical formulations using bromphenol blue
Apstrakt
A sensitive spectrophotometric method for the determination of bifonazole is described. Bifonazole was treated with bromphenol blue (BPB) in the presence of citrate buffer (pH 2.5; μ = 0.23). The formed ion-pair complex was extracted with chloroform. By using spectrophotometric measurements, Job's and Bent-French's methods, it was found that bifonazole and BPB formed an ion-pair complex with the absorption maximum at 414 nm (molecular ratio of bifonazole BPB = 1:2) and molar absorptivity 2.23 x 104 L mol-1 cm-1. Beer's law was obeyed up to 2.5 μg ml-1. The proposed method was applied for determination of bifonazole in pharmaceutical formulations. Statistical analysis of the results obtained showed that the proposed method has good reproducibility and precision.
Izvor:
Farmaco, 1993, 48, 7, 1007-1015Institucija/grupa
PharmacyTY - JOUR AU - Vladimirov, S AU - Brborić, Jasmina AU - Agbaba, Danica AU - Zivanov, D AU - Stakić, D.Ž PY - 1993 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/105 AB - A sensitive spectrophotometric method for the determination of bifonazole is described. Bifonazole was treated with bromphenol blue (BPB) in the presence of citrate buffer (pH 2.5; μ = 0.23). The formed ion-pair complex was extracted with chloroform. By using spectrophotometric measurements, Job's and Bent-French's methods, it was found that bifonazole and BPB formed an ion-pair complex with the absorption maximum at 414 nm (molecular ratio of bifonazole BPB = 1:2) and molar absorptivity 2.23 x 104 L mol-1 cm-1. Beer's law was obeyed up to 2.5 μg ml-1. The proposed method was applied for determination of bifonazole in pharmaceutical formulations. Statistical analysis of the results obtained showed that the proposed method has good reproducibility and precision. T2 - Farmaco T1 - Spectrophotometric determination of bifonazole in pharmaceutical formulations using bromphenol blue VL - 48 IS - 7 SP - 1007 EP - 1015 UR - https://hdl.handle.net/21.15107/rcub_farfar_105 ER -
@article{ author = "Vladimirov, S and Brborić, Jasmina and Agbaba, Danica and Zivanov, D and Stakić, D.Ž", year = "1993", abstract = "A sensitive spectrophotometric method for the determination of bifonazole is described. Bifonazole was treated with bromphenol blue (BPB) in the presence of citrate buffer (pH 2.5; μ = 0.23). The formed ion-pair complex was extracted with chloroform. By using spectrophotometric measurements, Job's and Bent-French's methods, it was found that bifonazole and BPB formed an ion-pair complex with the absorption maximum at 414 nm (molecular ratio of bifonazole BPB = 1:2) and molar absorptivity 2.23 x 104 L mol-1 cm-1. Beer's law was obeyed up to 2.5 μg ml-1. The proposed method was applied for determination of bifonazole in pharmaceutical formulations. Statistical analysis of the results obtained showed that the proposed method has good reproducibility and precision.", journal = "Farmaco", title = "Spectrophotometric determination of bifonazole in pharmaceutical formulations using bromphenol blue", volume = "48", number = "7", pages = "1007-1015", url = "https://hdl.handle.net/21.15107/rcub_farfar_105" }
Vladimirov, S., Brborić, J., Agbaba, D., Zivanov, D.,& Stakić, D.Ž. (1993). Spectrophotometric determination of bifonazole in pharmaceutical formulations using bromphenol blue. in Farmaco, 48(7), 1007-1015. https://hdl.handle.net/21.15107/rcub_farfar_105
Vladimirov S, Brborić J, Agbaba D, Zivanov D, Stakić D. Spectrophotometric determination of bifonazole in pharmaceutical formulations using bromphenol blue. in Farmaco. 1993;48(7):1007-1015. https://hdl.handle.net/21.15107/rcub_farfar_105 .
Vladimirov, S, Brborić, Jasmina, Agbaba, Danica, Zivanov, D, Stakić, D.Ž, "Spectrophotometric determination of bifonazole in pharmaceutical formulations using bromphenol blue" in Farmaco, 48, no. 7 (1993):1007-1015, https://hdl.handle.net/21.15107/rcub_farfar_105 .