Statistical Optimization of Reverse Phase High Performance Liquid Chromatography for the Analysis of Caffeine Paracetamol and its Degradation Product p-aminophenol
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Paracetamol, analgoantipyretic and anti-inflammatory drug, is available in numerous pharmaceutical formulations in common combination with caffeine and some other drug substances. p-aminophenol is paracatamol's process-related impurity that also may be present in formulations containing paracetamol. This paper presents a RP-HPLC method for simultaneous determination of caffeine, paracetamol and p-aminophenol, using chromatographic system Hewlett Packard 1100 series. In defining optimal RP-HPLC chromatographic conditions for the separation of these three compounds, experimental design was applied. Completely separation was achieved using Zorbax Extend C18 column (150 mm x 4.6 mm, 5 mu m), with mobile phase consisting of methanol-phosphate buffer pH 6 (20:80 v/v), flow rate of 1 ml/min, and column temperature of 30 degrees C. UV detection was performed at 230 nm.
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RP-HPLC / caffeine / paracetamol / p-aminophenol / statistical optimizationSource:
Acta Chimica Slovenica, 2008, 55, 3, 665-670Publisher:
- Slovensko Kemijsko Drustvo, Ljubljana
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PharmacyTY - JOUR AU - Crevar, Milkica AU - Ivković, Branka AU - Vladimirov, Sote AU - Kuntić, Vesna AU - Vujić, Zorica PY - 2008 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1064 AB - Paracetamol, analgoantipyretic and anti-inflammatory drug, is available in numerous pharmaceutical formulations in common combination with caffeine and some other drug substances. p-aminophenol is paracatamol's process-related impurity that also may be present in formulations containing paracetamol. This paper presents a RP-HPLC method for simultaneous determination of caffeine, paracetamol and p-aminophenol, using chromatographic system Hewlett Packard 1100 series. In defining optimal RP-HPLC chromatographic conditions for the separation of these three compounds, experimental design was applied. Completely separation was achieved using Zorbax Extend C18 column (150 mm x 4.6 mm, 5 mu m), with mobile phase consisting of methanol-phosphate buffer pH 6 (20:80 v/v), flow rate of 1 ml/min, and column temperature of 30 degrees C. UV detection was performed at 230 nm. PB - Slovensko Kemijsko Drustvo, Ljubljana T2 - Acta Chimica Slovenica T1 - Statistical Optimization of Reverse Phase High Performance Liquid Chromatography for the Analysis of Caffeine Paracetamol and its Degradation Product p-aminophenol VL - 55 IS - 3 SP - 665 EP - 670 UR - https://hdl.handle.net/21.15107/rcub_farfar_1064 ER -
@article{ author = "Crevar, Milkica and Ivković, Branka and Vladimirov, Sote and Kuntić, Vesna and Vujić, Zorica", year = "2008", abstract = "Paracetamol, analgoantipyretic and anti-inflammatory drug, is available in numerous pharmaceutical formulations in common combination with caffeine and some other drug substances. p-aminophenol is paracatamol's process-related impurity that also may be present in formulations containing paracetamol. This paper presents a RP-HPLC method for simultaneous determination of caffeine, paracetamol and p-aminophenol, using chromatographic system Hewlett Packard 1100 series. In defining optimal RP-HPLC chromatographic conditions for the separation of these three compounds, experimental design was applied. Completely separation was achieved using Zorbax Extend C18 column (150 mm x 4.6 mm, 5 mu m), with mobile phase consisting of methanol-phosphate buffer pH 6 (20:80 v/v), flow rate of 1 ml/min, and column temperature of 30 degrees C. UV detection was performed at 230 nm.", publisher = "Slovensko Kemijsko Drustvo, Ljubljana", journal = "Acta Chimica Slovenica", title = "Statistical Optimization of Reverse Phase High Performance Liquid Chromatography for the Analysis of Caffeine Paracetamol and its Degradation Product p-aminophenol", volume = "55", number = "3", pages = "665-670", url = "https://hdl.handle.net/21.15107/rcub_farfar_1064" }
Crevar, M., Ivković, B., Vladimirov, S., Kuntić, V.,& Vujić, Z.. (2008). Statistical Optimization of Reverse Phase High Performance Liquid Chromatography for the Analysis of Caffeine Paracetamol and its Degradation Product p-aminophenol. in Acta Chimica Slovenica Slovensko Kemijsko Drustvo, Ljubljana., 55(3), 665-670. https://hdl.handle.net/21.15107/rcub_farfar_1064
Crevar M, Ivković B, Vladimirov S, Kuntić V, Vujić Z. Statistical Optimization of Reverse Phase High Performance Liquid Chromatography for the Analysis of Caffeine Paracetamol and its Degradation Product p-aminophenol. in Acta Chimica Slovenica. 2008;55(3):665-670. https://hdl.handle.net/21.15107/rcub_farfar_1064 .
Crevar, Milkica, Ivković, Branka, Vladimirov, Sote, Kuntić, Vesna, Vujić, Zorica, "Statistical Optimization of Reverse Phase High Performance Liquid Chromatography for the Analysis of Caffeine Paracetamol and its Degradation Product p-aminophenol" in Acta Chimica Slovenica, 55, no. 3 (2008):665-670, https://hdl.handle.net/21.15107/rcub_farfar_1064 .