Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor
Abstract
The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intrapar...ticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture.
Source:
Applied Microbiology and Biotechnology, 1995, 43, 3, 416-423Collections
Institution/Community
PharmacyTY - JOUR AU - Sajc, L AU - Vunjak-Novaković, G AU - Grubišić, Dragoljub AU - Kovačević, Nada AU - Vuković, D AU - Bugarski, Branko PY - 1995 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/126 AB - The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture. T2 - Applied Microbiology and Biotechnology T1 - Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor VL - 43 IS - 3 SP - 416 EP - 423 DO - 10.1007/BF00218443 ER -
@article{ author = "Sajc, L and Vunjak-Novaković, G and Grubišić, Dragoljub and Kovačević, Nada and Vuković, D and Bugarski, Branko", year = "1995", abstract = "The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture.", journal = "Applied Microbiology and Biotechnology", title = "Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor", volume = "43", number = "3", pages = "416-423", doi = "10.1007/BF00218443" }
Sajc, L., Vunjak-Novaković, G., Grubišić, D., Kovačević, N., Vuković, D.,& Bugarski, B.. (1995). Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor. in Applied Microbiology and Biotechnology, 43(3), 416-423. https://doi.org/10.1007/BF00218443
Sajc L, Vunjak-Novaković G, Grubišić D, Kovačević N, Vuković D, Bugarski B. Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor. in Applied Microbiology and Biotechnology. 1995;43(3):416-423. doi:10.1007/BF00218443 .
Sajc, L, Vunjak-Novaković, G, Grubišić, Dragoljub, Kovačević, Nada, Vuković, D, Bugarski, Branko, "Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor" in Applied Microbiology and Biotechnology, 43, no. 3 (1995):416-423, https://doi.org/10.1007/BF00218443 . .