Determination of 8-hydroxyquinoline sulfate in tuberculin solutions by planar and high performance liquid chromatography
Abstract
TLC and HPLC methods for the determination of the preservative, 8-hydroxyquinoline sulfate in PPD-T tuberculin solution were developed. The planar chromatography method involved separation of 8-hydroxyquinoline sulfate on a TLC plate using a butyl-acetate: formic acid :2-propanol mobile phase, detection and quantitation by densitometric scanning. The HPLC method was on a LiChrosorb RP-18 column with acetonitrile-water (65:35 v/v) mobile phase, adjusted to pH 3.05 by phosphoric acid. Linearity, reproducibility and accuracy were found to be satisfactory. Under selected conditions, the limit of detection (LOD) of both methods was similar - about 25 ng.
Source:
Chromatographia, 1997, 45, 145-148Publisher:
- Vieweg, Wiesbaden
DOI: 10.1007/BF02505552
ISSN: 0009-5893
WoS: A1997XA09900024
Scopus: 2-s2.0-0030995068
Collections
Institution/Community
PharmacyTY - JOUR AU - Agbaba, Danica AU - Mihajlović, A AU - Beljanski, P AU - Živanov-Stakić, Dobrila AU - Vladimirov, S PY - 1997 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/146 AB - TLC and HPLC methods for the determination of the preservative, 8-hydroxyquinoline sulfate in PPD-T tuberculin solution were developed. The planar chromatography method involved separation of 8-hydroxyquinoline sulfate on a TLC plate using a butyl-acetate: formic acid :2-propanol mobile phase, detection and quantitation by densitometric scanning. The HPLC method was on a LiChrosorb RP-18 column with acetonitrile-water (65:35 v/v) mobile phase, adjusted to pH 3.05 by phosphoric acid. Linearity, reproducibility and accuracy were found to be satisfactory. Under selected conditions, the limit of detection (LOD) of both methods was similar - about 25 ng. PB - Vieweg, Wiesbaden T2 - Chromatographia T1 - Determination of 8-hydroxyquinoline sulfate in tuberculin solutions by planar and high performance liquid chromatography VL - 45 SP - 145 EP - 148 DO - 10.1007/BF02505552 ER -
@article{ author = "Agbaba, Danica and Mihajlović, A and Beljanski, P and Živanov-Stakić, Dobrila and Vladimirov, S", year = "1997", abstract = "TLC and HPLC methods for the determination of the preservative, 8-hydroxyquinoline sulfate in PPD-T tuberculin solution were developed. The planar chromatography method involved separation of 8-hydroxyquinoline sulfate on a TLC plate using a butyl-acetate: formic acid :2-propanol mobile phase, detection and quantitation by densitometric scanning. The HPLC method was on a LiChrosorb RP-18 column with acetonitrile-water (65:35 v/v) mobile phase, adjusted to pH 3.05 by phosphoric acid. Linearity, reproducibility and accuracy were found to be satisfactory. Under selected conditions, the limit of detection (LOD) of both methods was similar - about 25 ng.", publisher = "Vieweg, Wiesbaden", journal = "Chromatographia", title = "Determination of 8-hydroxyquinoline sulfate in tuberculin solutions by planar and high performance liquid chromatography", volume = "45", pages = "145-148", doi = "10.1007/BF02505552" }
Agbaba, D., Mihajlović, A., Beljanski, P., Živanov-Stakić, D.,& Vladimirov, S.. (1997). Determination of 8-hydroxyquinoline sulfate in tuberculin solutions by planar and high performance liquid chromatography. in Chromatographia Vieweg, Wiesbaden., 45, 145-148. https://doi.org/10.1007/BF02505552
Agbaba D, Mihajlović A, Beljanski P, Živanov-Stakić D, Vladimirov S. Determination of 8-hydroxyquinoline sulfate in tuberculin solutions by planar and high performance liquid chromatography. in Chromatographia. 1997;45:145-148. doi:10.1007/BF02505552 .
Agbaba, Danica, Mihajlović, A, Beljanski, P, Živanov-Stakić, Dobrila, Vladimirov, S, "Determination of 8-hydroxyquinoline sulfate in tuberculin solutions by planar and high performance liquid chromatography" in Chromatographia, 45 (1997):145-148, https://doi.org/10.1007/BF02505552 . .