Ultra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry of single peptides of angiotensin-converting enzyme inhibitors
Само за регистроване кориснике
2011
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The separation of structurally related angiotensin-converting enzyme (ACE) inhibitors lisinopril, cilazapril, ramipril and quinapril and their corresponding active diacid forms (prilates) by conventional TLC silica gel 60 plates was contrasted with that afforded by monolithic ultra-thin-layer chromatographic (UTLC) plates. For the use of UTLC plates technical modifications of the commercially available equipments for the sample application, development and detection were made. Plates were developed in modified horizontal developing chamber using ethyl acetate-acetone-acetic acid-water (4:1:0.25:0.5, v/v). Detection of the separated compounds was performed densitometrically in absorption/reflectance mode at 220 nm and after exposure to iodine also by image analysis. The obtained results showed that monolithic layer is more efficient for the separation of structurally similar polar compounds, such as prilates than conventional silica layers. Identification of the compounds was confirmed ...by ESI-MS after their on-line extraction from the UTLC and TLC plates by means of Camag TLC-MS interface.
Кључне речи:
UTLC / UTLC-MS / TLC / TLC-MS / Densitometry / Image analysis / Prils / PrilatesИзвор:
Journal of Chromatography A, 2011, 1218, 20, 3089-3094Издавач:
- Elsevier Science BV, Amsterdam
Финансирање / пројекти:
- Slovenian Research Agency
- EN(Right arrow)FIST Centre of Excellence, Slovenia
DOI: 10.1016/j.chroma.2011.03.039
ISSN: 0021-9673
PubMed: 21496825
WoS: 000291065500034
Scopus: 2-s2.0-79955124869
Институција/група
PharmacyTY - JOUR AU - Vovk, Irena AU - Popović, Gordana AU - Simonovska, Breda AU - Albreht, Alen AU - Agbaba, Danica PY - 2011 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1570 AB - The separation of structurally related angiotensin-converting enzyme (ACE) inhibitors lisinopril, cilazapril, ramipril and quinapril and their corresponding active diacid forms (prilates) by conventional TLC silica gel 60 plates was contrasted with that afforded by monolithic ultra-thin-layer chromatographic (UTLC) plates. For the use of UTLC plates technical modifications of the commercially available equipments for the sample application, development and detection were made. Plates were developed in modified horizontal developing chamber using ethyl acetate-acetone-acetic acid-water (4:1:0.25:0.5, v/v). Detection of the separated compounds was performed densitometrically in absorption/reflectance mode at 220 nm and after exposure to iodine also by image analysis. The obtained results showed that monolithic layer is more efficient for the separation of structurally similar polar compounds, such as prilates than conventional silica layers. Identification of the compounds was confirmed by ESI-MS after their on-line extraction from the UTLC and TLC plates by means of Camag TLC-MS interface. PB - Elsevier Science BV, Amsterdam T2 - Journal of Chromatography A T1 - Ultra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry of single peptides of angiotensin-converting enzyme inhibitors VL - 1218 IS - 20 SP - 3089 EP - 3094 DO - 10.1016/j.chroma.2011.03.039 ER -
@article{ author = "Vovk, Irena and Popović, Gordana and Simonovska, Breda and Albreht, Alen and Agbaba, Danica", year = "2011", abstract = "The separation of structurally related angiotensin-converting enzyme (ACE) inhibitors lisinopril, cilazapril, ramipril and quinapril and their corresponding active diacid forms (prilates) by conventional TLC silica gel 60 plates was contrasted with that afforded by monolithic ultra-thin-layer chromatographic (UTLC) plates. For the use of UTLC plates technical modifications of the commercially available equipments for the sample application, development and detection were made. Plates were developed in modified horizontal developing chamber using ethyl acetate-acetone-acetic acid-water (4:1:0.25:0.5, v/v). Detection of the separated compounds was performed densitometrically in absorption/reflectance mode at 220 nm and after exposure to iodine also by image analysis. The obtained results showed that monolithic layer is more efficient for the separation of structurally similar polar compounds, such as prilates than conventional silica layers. Identification of the compounds was confirmed by ESI-MS after their on-line extraction from the UTLC and TLC plates by means of Camag TLC-MS interface.", publisher = "Elsevier Science BV, Amsterdam", journal = "Journal of Chromatography A", title = "Ultra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry of single peptides of angiotensin-converting enzyme inhibitors", volume = "1218", number = "20", pages = "3089-3094", doi = "10.1016/j.chroma.2011.03.039" }
Vovk, I., Popović, G., Simonovska, B., Albreht, A.,& Agbaba, D.. (2011). Ultra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry of single peptides of angiotensin-converting enzyme inhibitors. in Journal of Chromatography A Elsevier Science BV, Amsterdam., 1218(20), 3089-3094. https://doi.org/10.1016/j.chroma.2011.03.039
Vovk I, Popović G, Simonovska B, Albreht A, Agbaba D. Ultra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry of single peptides of angiotensin-converting enzyme inhibitors. in Journal of Chromatography A. 2011;1218(20):3089-3094. doi:10.1016/j.chroma.2011.03.039 .
Vovk, Irena, Popović, Gordana, Simonovska, Breda, Albreht, Alen, Agbaba, Danica, "Ultra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry of single peptides of angiotensin-converting enzyme inhibitors" in Journal of Chromatography A, 1218, no. 20 (2011):3089-3094, https://doi.org/10.1016/j.chroma.2011.03.039 . .