The Lipophilicity Examination of Some ACE inhibitors and Hydrochlorothiazide on Cellulose in RP Thin-Layer Chromatography
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Odović, JadrankaKarljiković-Rajić, Katarina
Trbojević-Stanković, Jasna
Stojimirović, Biljana
Vladimirov, Sote
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In this assay, the evaluation of lipophilicity of four ACE-inhibitors and hydrochlorothiazide (HCTZ) with RP-TLC on cellulose layers was described using three binary solvent systems. The selected ACE inhibitors had sufficiently different structures which can indicate the method suitability for their lipophilicity evaluation as the model substances in comparison with HCTZ. In addition, the linear relationship between the R-M-values and composition of mobile phases was established in the current study. From the regression data of the plots, the hydrophobicity parameters, R-M(0) and m, were determined and C-0 parameter was calculated. The correlations between the experimentally obtained hydrophobicity parameters and calculated log p values were studied. Furthermore, the obtained results were compared with those previously obtained on RP-18 modified silica gel. Very good correlation (r = 0.91; water-ethanol solvent system) between the chromatographically obtained hydrophobicity parameters ...and calculated log p values confirmed the selection of ACE inhibitors since lisinopril and quinapril were on the opposite sites of linear relationship. The results indicate that cellulose as an easily available sorbent can be successfully used for the lipophilicity investigation of examined substances with RP-TLC.
Keywords:
Lipophilicity / ACE inhibitors / Hydrochlorothiazide / Thin-Layer ChromatographySource:
Iranian Journal of Pharmaceutical Research, 2012, 11, 3, 763-770Publisher:
- Shaheed Beheshti Univ, Sch Pharmacy, Tehran
Funding / projects:
- Development of molecules with antiinflammatory and cardioprotective activity: structural modifications, modelling, physicochemical characterization and formulation investigations (RS-MESTD-Basic Research (BR or ON)-172041)
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PharmacyTY - JOUR AU - Odović, Jadranka AU - Karljiković-Rajić, Katarina AU - Trbojević-Stanković, Jasna AU - Stojimirović, Biljana AU - Vladimirov, Sote PY - 2012 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1642 AB - In this assay, the evaluation of lipophilicity of four ACE-inhibitors and hydrochlorothiazide (HCTZ) with RP-TLC on cellulose layers was described using three binary solvent systems. The selected ACE inhibitors had sufficiently different structures which can indicate the method suitability for their lipophilicity evaluation as the model substances in comparison with HCTZ. In addition, the linear relationship between the R-M-values and composition of mobile phases was established in the current study. From the regression data of the plots, the hydrophobicity parameters, R-M(0) and m, were determined and C-0 parameter was calculated. The correlations between the experimentally obtained hydrophobicity parameters and calculated log p values were studied. Furthermore, the obtained results were compared with those previously obtained on RP-18 modified silica gel. Very good correlation (r = 0.91; water-ethanol solvent system) between the chromatographically obtained hydrophobicity parameters and calculated log p values confirmed the selection of ACE inhibitors since lisinopril and quinapril were on the opposite sites of linear relationship. The results indicate that cellulose as an easily available sorbent can be successfully used for the lipophilicity investigation of examined substances with RP-TLC. PB - Shaheed Beheshti Univ, Sch Pharmacy, Tehran T2 - Iranian Journal of Pharmaceutical Research T1 - The Lipophilicity Examination of Some ACE inhibitors and Hydrochlorothiazide on Cellulose in RP Thin-Layer Chromatography VL - 11 IS - 3 SP - 763 EP - 770 UR - https://hdl.handle.net/21.15107/rcub_farfar_1642 ER -
@article{ author = "Odović, Jadranka and Karljiković-Rajić, Katarina and Trbojević-Stanković, Jasna and Stojimirović, Biljana and Vladimirov, Sote", year = "2012", abstract = "In this assay, the evaluation of lipophilicity of four ACE-inhibitors and hydrochlorothiazide (HCTZ) with RP-TLC on cellulose layers was described using three binary solvent systems. The selected ACE inhibitors had sufficiently different structures which can indicate the method suitability for their lipophilicity evaluation as the model substances in comparison with HCTZ. In addition, the linear relationship between the R-M-values and composition of mobile phases was established in the current study. From the regression data of the plots, the hydrophobicity parameters, R-M(0) and m, were determined and C-0 parameter was calculated. The correlations between the experimentally obtained hydrophobicity parameters and calculated log p values were studied. Furthermore, the obtained results were compared with those previously obtained on RP-18 modified silica gel. Very good correlation (r = 0.91; water-ethanol solvent system) between the chromatographically obtained hydrophobicity parameters and calculated log p values confirmed the selection of ACE inhibitors since lisinopril and quinapril were on the opposite sites of linear relationship. The results indicate that cellulose as an easily available sorbent can be successfully used for the lipophilicity investigation of examined substances with RP-TLC.", publisher = "Shaheed Beheshti Univ, Sch Pharmacy, Tehran", journal = "Iranian Journal of Pharmaceutical Research", title = "The Lipophilicity Examination of Some ACE inhibitors and Hydrochlorothiazide on Cellulose in RP Thin-Layer Chromatography", volume = "11", number = "3", pages = "763-770", url = "https://hdl.handle.net/21.15107/rcub_farfar_1642" }
Odović, J., Karljiković-Rajić, K., Trbojević-Stanković, J., Stojimirović, B.,& Vladimirov, S.. (2012). The Lipophilicity Examination of Some ACE inhibitors and Hydrochlorothiazide on Cellulose in RP Thin-Layer Chromatography. in Iranian Journal of Pharmaceutical Research Shaheed Beheshti Univ, Sch Pharmacy, Tehran., 11(3), 763-770. https://hdl.handle.net/21.15107/rcub_farfar_1642
Odović J, Karljiković-Rajić K, Trbojević-Stanković J, Stojimirović B, Vladimirov S. The Lipophilicity Examination of Some ACE inhibitors and Hydrochlorothiazide on Cellulose in RP Thin-Layer Chromatography. in Iranian Journal of Pharmaceutical Research. 2012;11(3):763-770. https://hdl.handle.net/21.15107/rcub_farfar_1642 .
Odović, Jadranka, Karljiković-Rajić, Katarina, Trbojević-Stanković, Jasna, Stojimirović, Biljana, Vladimirov, Sote, "The Lipophilicity Examination of Some ACE inhibitors and Hydrochlorothiazide on Cellulose in RP Thin-Layer Chromatography" in Iranian Journal of Pharmaceutical Research, 11, no. 3 (2012):763-770, https://hdl.handle.net/21.15107/rcub_farfar_1642 .