Simultaneous Analysis of Irbesartan and Hydrochlorothiazide: An Improved HPLC Method with the Aid of a Chemometric Protocol
Апстракт
Experimental design method was used for HPLC determination of irbesartan and hydrochlorothiazide in combined dosage forms. The traditional approach for optimization of experiments is time-consuming, involves a large number of runs and does not allow establishing the multiple interacting parameters. The main advantages of the experimental design method include the simultaneous screening of a larger number of factors affecting response and the estimation of possible interactions. On the basis of preliminary experiments, three factors-independent variables were selected as inputs (methanol content, pH of the mobile phase and temperature) and as dependent variables, five responses (resolution, symmetry of irbesartan peak, symmetry of hydrochlorothiazide peak, retention factor of irbesartan and retention factor of hydrochlorothiazide) were chosen. A full 2(3) factorial design, where factors were examined at two different levels ("low" and "high") was used to determine which factors had an e...ffect on the studied response. Afterwards, experimental design was used to optimize these influent parameters in the previously selected experimental domain. The novelty of our method lies in the optimization step accomplished by Derringer's desirability function. After optimizing the experimental conditions a separation was conducted on a Supelcosil C-18 (150 mm x 4.6 mm, 5 mu m particle size) column with a mobile phase consisting of methanol-tetrahydrofuran-acetate buffer 47:10:43 v/v/v, pH 6.5 and a column temperature of 25 degrees C. The developed method was successfully applied to the simultaneous separation of these drug-active compounds in their commercial pharmaceutical dosage forms.
Кључне речи:
HPLC / experimental design / irbesartan / hydrochlorothiazideИзвор:
Molecules, 2012, 17, 3, 3461-3474Издавач:
- MDPI, Basel
Финансирање / пројекти:
- Развој молекула са антиинфламаторним и кардиопроактивним дејством: структурне модификације, моделовање, физичкохемијска карактеризација и формулациона испитивања (RS-MESTD-Basic Research (BR or ON)-172041)
DOI: 10.3390/molecules17033461
ISSN: 1420-3049
PubMed: 22426527
WoS: 000302120600074
Scopus: 2-s2.0-84858958219
Институција/група
PharmacyTY - JOUR AU - Vujić, Zorica AU - Mulavdić, Nedzad AU - Smajić, Miralem AU - Brborić, Jasmina AU - Stanković, Predrag PY - 2012 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1703 AB - Experimental design method was used for HPLC determination of irbesartan and hydrochlorothiazide in combined dosage forms. The traditional approach for optimization of experiments is time-consuming, involves a large number of runs and does not allow establishing the multiple interacting parameters. The main advantages of the experimental design method include the simultaneous screening of a larger number of factors affecting response and the estimation of possible interactions. On the basis of preliminary experiments, three factors-independent variables were selected as inputs (methanol content, pH of the mobile phase and temperature) and as dependent variables, five responses (resolution, symmetry of irbesartan peak, symmetry of hydrochlorothiazide peak, retention factor of irbesartan and retention factor of hydrochlorothiazide) were chosen. A full 2(3) factorial design, where factors were examined at two different levels ("low" and "high") was used to determine which factors had an effect on the studied response. Afterwards, experimental design was used to optimize these influent parameters in the previously selected experimental domain. The novelty of our method lies in the optimization step accomplished by Derringer's desirability function. After optimizing the experimental conditions a separation was conducted on a Supelcosil C-18 (150 mm x 4.6 mm, 5 mu m particle size) column with a mobile phase consisting of methanol-tetrahydrofuran-acetate buffer 47:10:43 v/v/v, pH 6.5 and a column temperature of 25 degrees C. The developed method was successfully applied to the simultaneous separation of these drug-active compounds in their commercial pharmaceutical dosage forms. PB - MDPI, Basel T2 - Molecules T1 - Simultaneous Analysis of Irbesartan and Hydrochlorothiazide: An Improved HPLC Method with the Aid of a Chemometric Protocol VL - 17 IS - 3 SP - 3461 EP - 3474 DO - 10.3390/molecules17033461 ER -
@article{ author = "Vujić, Zorica and Mulavdić, Nedzad and Smajić, Miralem and Brborić, Jasmina and Stanković, Predrag", year = "2012", abstract = "Experimental design method was used for HPLC determination of irbesartan and hydrochlorothiazide in combined dosage forms. The traditional approach for optimization of experiments is time-consuming, involves a large number of runs and does not allow establishing the multiple interacting parameters. The main advantages of the experimental design method include the simultaneous screening of a larger number of factors affecting response and the estimation of possible interactions. On the basis of preliminary experiments, three factors-independent variables were selected as inputs (methanol content, pH of the mobile phase and temperature) and as dependent variables, five responses (resolution, symmetry of irbesartan peak, symmetry of hydrochlorothiazide peak, retention factor of irbesartan and retention factor of hydrochlorothiazide) were chosen. A full 2(3) factorial design, where factors were examined at two different levels ("low" and "high") was used to determine which factors had an effect on the studied response. Afterwards, experimental design was used to optimize these influent parameters in the previously selected experimental domain. The novelty of our method lies in the optimization step accomplished by Derringer's desirability function. After optimizing the experimental conditions a separation was conducted on a Supelcosil C-18 (150 mm x 4.6 mm, 5 mu m particle size) column with a mobile phase consisting of methanol-tetrahydrofuran-acetate buffer 47:10:43 v/v/v, pH 6.5 and a column temperature of 25 degrees C. The developed method was successfully applied to the simultaneous separation of these drug-active compounds in their commercial pharmaceutical dosage forms.", publisher = "MDPI, Basel", journal = "Molecules", title = "Simultaneous Analysis of Irbesartan and Hydrochlorothiazide: An Improved HPLC Method with the Aid of a Chemometric Protocol", volume = "17", number = "3", pages = "3461-3474", doi = "10.3390/molecules17033461" }
Vujić, Z., Mulavdić, N., Smajić, M., Brborić, J.,& Stanković, P.. (2012). Simultaneous Analysis of Irbesartan and Hydrochlorothiazide: An Improved HPLC Method with the Aid of a Chemometric Protocol. in Molecules MDPI, Basel., 17(3), 3461-3474. https://doi.org/10.3390/molecules17033461
Vujić Z, Mulavdić N, Smajić M, Brborić J, Stanković P. Simultaneous Analysis of Irbesartan and Hydrochlorothiazide: An Improved HPLC Method with the Aid of a Chemometric Protocol. in Molecules. 2012;17(3):3461-3474. doi:10.3390/molecules17033461 .
Vujić, Zorica, Mulavdić, Nedzad, Smajić, Miralem, Brborić, Jasmina, Stanković, Predrag, "Simultaneous Analysis of Irbesartan and Hydrochlorothiazide: An Improved HPLC Method with the Aid of a Chemometric Protocol" in Molecules, 17, no. 3 (2012):3461-3474, https://doi.org/10.3390/molecules17033461 . .