In vivo and in vitro antioxidant effects of three Veronica species
Апстракт
The aim of the present study was to examine the antioxidant activity of three Veronica species (Plantaginaceae). The antioxidant potential of various extracts obtained from aerial flowering parts was evaluated by DPPH-free (1,1-diphenyl-2-picrylhydrazyl-free) radical scavenging activity and ferric-reducing antioxidant power assays. Considerable antioxidant activity was observed in the plant samples (FRAP values ranged from 0.97 to 4.85 mmol Fe2+/g, and DPPH IC50 values from 12.58 to 66.34 A mu g/ml); however, these levels were lower than the activity of the control compound butylated hydroxytoluene (BHT) (FRAP: 10.58 mmol Fe2+/g; DPPH IC50: 9.57 A mu g/ml). Also, the in vivo antioxidant effects were evaluated in several hepatic antioxidant systems in rats (activities of glutathione peroxidase, glutathione reductase, peroxidase, catalase, xanthine oxidase, glutathione content and level of thiobarbituric acid reactive substances) after treatment with different Veronica extracts, or in co...mbination with carbon tetrachloride (CCl4). Pretreatment with 100 mg/kg b.w. of Veronica extracts inhibited CCl4-induced liver injury by decreasing TBA-RS level, increasing GSH content, and bringing the activities of CAT and Px to control levels. The present study suggests that the extracts analyzed could protect the liver cells from CCl4-induced liver damage by their antioxidative effect on hepatocytes.
Кључне речи:
Antioxidant activity / Veronica species / Carbon tetrachloride / Lipid peroxidation / Biochemical parametersИзвор:
Central European Journal of Biology, 2012, 7, 3, 559-568Издавач:
- Versita, Warsaw
Финансирање / пројекти:
- Испитивање лековитог потенцијала биљака: морфолошка, хемијска и фармаколошка карактеризација (RS-MESTD-MPN2006-2010-143012)
DOI: 10.2478/s11535-012-0041-4
ISSN: 1895-104X
WoS: 000302476900020
Scopus: 2-s2.0-84859327011
Институција/група
PharmacyTY - JOUR AU - Živković, Jelena AU - Cebović, Tatjana AU - Maksimović, Zoran PY - 2012 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1707 AB - The aim of the present study was to examine the antioxidant activity of three Veronica species (Plantaginaceae). The antioxidant potential of various extracts obtained from aerial flowering parts was evaluated by DPPH-free (1,1-diphenyl-2-picrylhydrazyl-free) radical scavenging activity and ferric-reducing antioxidant power assays. Considerable antioxidant activity was observed in the plant samples (FRAP values ranged from 0.97 to 4.85 mmol Fe2+/g, and DPPH IC50 values from 12.58 to 66.34 A mu g/ml); however, these levels were lower than the activity of the control compound butylated hydroxytoluene (BHT) (FRAP: 10.58 mmol Fe2+/g; DPPH IC50: 9.57 A mu g/ml). Also, the in vivo antioxidant effects were evaluated in several hepatic antioxidant systems in rats (activities of glutathione peroxidase, glutathione reductase, peroxidase, catalase, xanthine oxidase, glutathione content and level of thiobarbituric acid reactive substances) after treatment with different Veronica extracts, or in combination with carbon tetrachloride (CCl4). Pretreatment with 100 mg/kg b.w. of Veronica extracts inhibited CCl4-induced liver injury by decreasing TBA-RS level, increasing GSH content, and bringing the activities of CAT and Px to control levels. The present study suggests that the extracts analyzed could protect the liver cells from CCl4-induced liver damage by their antioxidative effect on hepatocytes. PB - Versita, Warsaw T2 - Central European Journal of Biology T1 - In vivo and in vitro antioxidant effects of three Veronica species VL - 7 IS - 3 SP - 559 EP - 568 DO - 10.2478/s11535-012-0041-4 ER -
@article{ author = "Živković, Jelena and Cebović, Tatjana and Maksimović, Zoran", year = "2012", abstract = "The aim of the present study was to examine the antioxidant activity of three Veronica species (Plantaginaceae). The antioxidant potential of various extracts obtained from aerial flowering parts was evaluated by DPPH-free (1,1-diphenyl-2-picrylhydrazyl-free) radical scavenging activity and ferric-reducing antioxidant power assays. Considerable antioxidant activity was observed in the plant samples (FRAP values ranged from 0.97 to 4.85 mmol Fe2+/g, and DPPH IC50 values from 12.58 to 66.34 A mu g/ml); however, these levels were lower than the activity of the control compound butylated hydroxytoluene (BHT) (FRAP: 10.58 mmol Fe2+/g; DPPH IC50: 9.57 A mu g/ml). Also, the in vivo antioxidant effects were evaluated in several hepatic antioxidant systems in rats (activities of glutathione peroxidase, glutathione reductase, peroxidase, catalase, xanthine oxidase, glutathione content and level of thiobarbituric acid reactive substances) after treatment with different Veronica extracts, or in combination with carbon tetrachloride (CCl4). Pretreatment with 100 mg/kg b.w. of Veronica extracts inhibited CCl4-induced liver injury by decreasing TBA-RS level, increasing GSH content, and bringing the activities of CAT and Px to control levels. The present study suggests that the extracts analyzed could protect the liver cells from CCl4-induced liver damage by their antioxidative effect on hepatocytes.", publisher = "Versita, Warsaw", journal = "Central European Journal of Biology", title = "In vivo and in vitro antioxidant effects of three Veronica species", volume = "7", number = "3", pages = "559-568", doi = "10.2478/s11535-012-0041-4" }
Živković, J., Cebović, T.,& Maksimović, Z.. (2012). In vivo and in vitro antioxidant effects of three Veronica species. in Central European Journal of Biology Versita, Warsaw., 7(3), 559-568. https://doi.org/10.2478/s11535-012-0041-4
Živković J, Cebović T, Maksimović Z. In vivo and in vitro antioxidant effects of three Veronica species. in Central European Journal of Biology. 2012;7(3):559-568. doi:10.2478/s11535-012-0041-4 .
Živković, Jelena, Cebović, Tatjana, Maksimović, Zoran, "In vivo and in vitro antioxidant effects of three Veronica species" in Central European Journal of Biology, 7, no. 3 (2012):559-568, https://doi.org/10.2478/s11535-012-0041-4 . .