Determination of midazolam in pharmaceutical dosage forms by second-order derivative ultraviolet spectrophotometry
Апстракт
A second-order derivative ultraviolet spectroscopic method for the determination of midazolam in the presence of maleic acid is described. The assays were carried out in borate buffer (pH 9.2) at a wavelength of 222 nm, which corresponds to the position of the peak of 2 D spectrum of midazolam and to the intersection point of the 2 D spectrum of maleic acid with the abscissa (zero-crossing point). These conditions were chosen as optimum on the basis of the knowledge of acid-base equilibria of midazolam and maleic acid and of investigations of the absorption and 2 D spectra of midazolam and maleic acid and their mixtures. A calibration graph was obtained in the range of midazolam concentrations of 0.25-2.5x10 -5 mol/dm 3 with a correlation coefficient of 0.99996. The method is simple and rapid, and was successfully applied to the determination of midazolam in the presence of maleic acid in laboratory mixtures and in commercial tablets containing midazolam in the maleate form.
Кључне речи:
Derivative spectrophotometry / Midazolam / Midazolam formulationИзвор:
Journal of the Serbian Chemical Society, 1997, 62, 8, 687-693Издавач:
- Srpsko hemijsko društvo, Beograd
Институција/група
PharmacyTY - JOUR AU - Pfendt, Lidija AU - Popović, Gordana AU - Stefanović, Violeta M. AU - Rajić, M.B PY - 1997 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/171 AB - A second-order derivative ultraviolet spectroscopic method for the determination of midazolam in the presence of maleic acid is described. The assays were carried out in borate buffer (pH 9.2) at a wavelength of 222 nm, which corresponds to the position of the peak of 2 D spectrum of midazolam and to the intersection point of the 2 D spectrum of maleic acid with the abscissa (zero-crossing point). These conditions were chosen as optimum on the basis of the knowledge of acid-base equilibria of midazolam and maleic acid and of investigations of the absorption and 2 D spectra of midazolam and maleic acid and their mixtures. A calibration graph was obtained in the range of midazolam concentrations of 0.25-2.5x10 -5 mol/dm 3 with a correlation coefficient of 0.99996. The method is simple and rapid, and was successfully applied to the determination of midazolam in the presence of maleic acid in laboratory mixtures and in commercial tablets containing midazolam in the maleate form. PB - Srpsko hemijsko društvo, Beograd T2 - Journal of the Serbian Chemical Society T1 - Determination of midazolam in pharmaceutical dosage forms by second-order derivative ultraviolet spectrophotometry VL - 62 IS - 8 SP - 687 EP - 693 UR - https://hdl.handle.net/21.15107/rcub_farfar_171 ER -
@article{ author = "Pfendt, Lidija and Popović, Gordana and Stefanović, Violeta M. and Rajić, M.B", year = "1997", abstract = "A second-order derivative ultraviolet spectroscopic method for the determination of midazolam in the presence of maleic acid is described. The assays were carried out in borate buffer (pH 9.2) at a wavelength of 222 nm, which corresponds to the position of the peak of 2 D spectrum of midazolam and to the intersection point of the 2 D spectrum of maleic acid with the abscissa (zero-crossing point). These conditions were chosen as optimum on the basis of the knowledge of acid-base equilibria of midazolam and maleic acid and of investigations of the absorption and 2 D spectra of midazolam and maleic acid and their mixtures. A calibration graph was obtained in the range of midazolam concentrations of 0.25-2.5x10 -5 mol/dm 3 with a correlation coefficient of 0.99996. The method is simple and rapid, and was successfully applied to the determination of midazolam in the presence of maleic acid in laboratory mixtures and in commercial tablets containing midazolam in the maleate form.", publisher = "Srpsko hemijsko društvo, Beograd", journal = "Journal of the Serbian Chemical Society", title = "Determination of midazolam in pharmaceutical dosage forms by second-order derivative ultraviolet spectrophotometry", volume = "62", number = "8", pages = "687-693", url = "https://hdl.handle.net/21.15107/rcub_farfar_171" }
Pfendt, L., Popović, G., Stefanović, V. M.,& Rajić, M.B. (1997). Determination of midazolam in pharmaceutical dosage forms by second-order derivative ultraviolet spectrophotometry. in Journal of the Serbian Chemical Society Srpsko hemijsko društvo, Beograd., 62(8), 687-693. https://hdl.handle.net/21.15107/rcub_farfar_171
Pfendt L, Popović G, Stefanović VM, Rajić M. Determination of midazolam in pharmaceutical dosage forms by second-order derivative ultraviolet spectrophotometry. in Journal of the Serbian Chemical Society. 1997;62(8):687-693. https://hdl.handle.net/21.15107/rcub_farfar_171 .
Pfendt, Lidija, Popović, Gordana, Stefanović, Violeta M., Rajić, M.B, "Determination of midazolam in pharmaceutical dosage forms by second-order derivative ultraviolet spectrophotometry" in Journal of the Serbian Chemical Society, 62, no. 8 (1997):687-693, https://hdl.handle.net/21.15107/rcub_farfar_171 .