Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells
2015
Аутори
Knežević, AŽivković, Lada
Stajić, Mirjana
Vukojević, Jelena
Milovanović, I
Potparević, Biljana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Trametes species have been used for thousands of years in traditional and conventional medicine for the treatment of various types of diseases. The goal was to evaluate possible antigenotoxic effects of mycelium and basidiocarp extracts of selected Trametes species and to assess dependence on their antioxidant potential. Trametes versicolor, T. hirsuta, and T. gibbosa were the species studied. Antigenotoxic potentials of extracts were assessed on human peripheral white blood cells with basidiocarp and mycelium extracts of the species. The alkaline comet test was used for detection of DNA strand breaks and alkali-labile sites, as well as the extent of DNA migration. DPPH assay was used to estimate antioxidative properties of extracts. Fruiting body extracts of T. versicolor and T. gibbosa as well as T. hirsuta extracts, except that at 20.0 mg/mL, were not genotoxic agents. T. versicolor extract had at 5.0 mg/mL the greatest antigenotoxic effect in both pre-and posttreatment of leukocyte...s. The mycelium extracts of the three species had no genotoxic activity and significant antigenotoxic effect against H lt inf>2 lt /inf>O lt inf>2 lt /inf>-induced DNA damage, both in pre-and posttreatment. The results suggest that extracts of these three species could be considered as strong antigenotoxic agents able to stimulate genoprotective response of cells.
Извор:
Scientific World Journal, 2015, 2015Издавач:
- Hindawi Publishing Corporation
Финансирање / пројекти:
- Карактеризација и примена метаболита гљива и утврђивање потенцијала нових биофунгицида (RS-MESTD-Basic Research (BR or ON)-173032)
- Аберације ћелијског циклуса и утицај оксидативног стреса на неуродегенеративне процесе и малигну трансформацију ћелије (RS-MESTD-Basic Research (BR or ON)-173034)
Институција/група
PharmacyTY - JOUR AU - Knežević, A AU - Živković, Lada AU - Stajić, Mirjana AU - Vukojević, Jelena AU - Milovanović, I AU - Potparević, Biljana PY - 2015 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2495 AB - Trametes species have been used for thousands of years in traditional and conventional medicine for the treatment of various types of diseases. The goal was to evaluate possible antigenotoxic effects of mycelium and basidiocarp extracts of selected Trametes species and to assess dependence on their antioxidant potential. Trametes versicolor, T. hirsuta, and T. gibbosa were the species studied. Antigenotoxic potentials of extracts were assessed on human peripheral white blood cells with basidiocarp and mycelium extracts of the species. The alkaline comet test was used for detection of DNA strand breaks and alkali-labile sites, as well as the extent of DNA migration. DPPH assay was used to estimate antioxidative properties of extracts. Fruiting body extracts of T. versicolor and T. gibbosa as well as T. hirsuta extracts, except that at 20.0 mg/mL, were not genotoxic agents. T. versicolor extract had at 5.0 mg/mL the greatest antigenotoxic effect in both pre-and posttreatment of leukocytes. The mycelium extracts of the three species had no genotoxic activity and significant antigenotoxic effect against H lt inf>2 lt /inf>O lt inf>2 lt /inf>-induced DNA damage, both in pre-and posttreatment. The results suggest that extracts of these three species could be considered as strong antigenotoxic agents able to stimulate genoprotective response of cells. PB - Hindawi Publishing Corporation T2 - Scientific World Journal T1 - Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells VL - 2015 DO - 10.1155/2015/146378 ER -
@article{ author = "Knežević, A and Živković, Lada and Stajić, Mirjana and Vukojević, Jelena and Milovanović, I and Potparević, Biljana", year = "2015", abstract = "Trametes species have been used for thousands of years in traditional and conventional medicine for the treatment of various types of diseases. The goal was to evaluate possible antigenotoxic effects of mycelium and basidiocarp extracts of selected Trametes species and to assess dependence on their antioxidant potential. Trametes versicolor, T. hirsuta, and T. gibbosa were the species studied. Antigenotoxic potentials of extracts were assessed on human peripheral white blood cells with basidiocarp and mycelium extracts of the species. The alkaline comet test was used for detection of DNA strand breaks and alkali-labile sites, as well as the extent of DNA migration. DPPH assay was used to estimate antioxidative properties of extracts. Fruiting body extracts of T. versicolor and T. gibbosa as well as T. hirsuta extracts, except that at 20.0 mg/mL, were not genotoxic agents. T. versicolor extract had at 5.0 mg/mL the greatest antigenotoxic effect in both pre-and posttreatment of leukocytes. The mycelium extracts of the three species had no genotoxic activity and significant antigenotoxic effect against H lt inf>2 lt /inf>O lt inf>2 lt /inf>-induced DNA damage, both in pre-and posttreatment. The results suggest that extracts of these three species could be considered as strong antigenotoxic agents able to stimulate genoprotective response of cells.", publisher = "Hindawi Publishing Corporation", journal = "Scientific World Journal", title = "Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells", volume = "2015", doi = "10.1155/2015/146378" }
Knežević, A., Živković, L., Stajić, M., Vukojević, J., Milovanović, I.,& Potparević, B.. (2015). Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells. in Scientific World Journal Hindawi Publishing Corporation., 2015. https://doi.org/10.1155/2015/146378
Knežević A, Živković L, Stajić M, Vukojević J, Milovanović I, Potparević B. Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells. in Scientific World Journal. 2015;2015. doi:10.1155/2015/146378 .
Knežević, A, Živković, Lada, Stajić, Mirjana, Vukojević, Jelena, Milovanović, I, Potparević, Biljana, "Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells" in Scientific World Journal, 2015 (2015), https://doi.org/10.1155/2015/146378 . .