Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method
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2016
Authors
Foivas, AnargyrosMalenović, Anđelija
Kostić, Nada
Božić, Marija
Knežević, Miroslav
Loukas, Yannis L.
Dotsikas, Yannis
Article (Published version)
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In the current study, a rapid and sensitive LC-QTOF-MS/MS method for the determination of brinzolamide in dried blood spots (DBS) was developed and validated. This novel sample collection, storage and transfer technique was suitable for analyzing a drug with high distribution into red blood cells and negligible plasma levels. The method included an isocratic mobile phase consisting of methanol and 10 mM ammonium formate (90:10, v/v) and detection in positive electrospray mode (ESI+). The flow rate was adjusted to 0.350 mL/min yielding retention times of 1.7 min for both brinzolamide and internal standard (IS) rabeprazole on a Cyano analytical column, respectively. The validation of the proposed method over the concentration range 0.500-20.0 mu g/mL was performed in compliance with EMEA and FDA guidelines, assessing all major performance characteristics. Inter- and intra- assay precisions were less than 14%, while inter- and intra- assay accuracies varied from 922 to 111%. No matrix eff...ect was observed and the mean brinzolamide extraction recovery was 93.5%. The method was successfully applied to real DBS samples from patients in steady state condition, receiving brinzolamide ophthalmic suspension 1% (w/v) for several months. Initial concentrations were corrected due to hematocrit effect, using image processing algorithm written in Matlab.
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Journal of Pharmaceutical and Biomedical Analysis, 2016, 119, 84-90Publisher:
- Elsevier Science BV, Amsterdam
DOI: 10.1016/j.jpba.2015.11.043
ISSN: 0731-7085
PubMed: 26669612
WoS: 000370211900012
Scopus: 2-s2.0-84949255283
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PharmacyTY - JOUR AU - Foivas, Anargyros AU - Malenović, Anđelija AU - Kostić, Nada AU - Božić, Marija AU - Knežević, Miroslav AU - Loukas, Yannis L. AU - Dotsikas, Yannis PY - 2016 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2566 AB - In the current study, a rapid and sensitive LC-QTOF-MS/MS method for the determination of brinzolamide in dried blood spots (DBS) was developed and validated. This novel sample collection, storage and transfer technique was suitable for analyzing a drug with high distribution into red blood cells and negligible plasma levels. The method included an isocratic mobile phase consisting of methanol and 10 mM ammonium formate (90:10, v/v) and detection in positive electrospray mode (ESI+). The flow rate was adjusted to 0.350 mL/min yielding retention times of 1.7 min for both brinzolamide and internal standard (IS) rabeprazole on a Cyano analytical column, respectively. The validation of the proposed method over the concentration range 0.500-20.0 mu g/mL was performed in compliance with EMEA and FDA guidelines, assessing all major performance characteristics. Inter- and intra- assay precisions were less than 14%, while inter- and intra- assay accuracies varied from 922 to 111%. No matrix effect was observed and the mean brinzolamide extraction recovery was 93.5%. The method was successfully applied to real DBS samples from patients in steady state condition, receiving brinzolamide ophthalmic suspension 1% (w/v) for several months. Initial concentrations were corrected due to hematocrit effect, using image processing algorithm written in Matlab. PB - Elsevier Science BV, Amsterdam T2 - Journal of Pharmaceutical and Biomedical Analysis T1 - Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method VL - 119 SP - 84 EP - 90 DO - 10.1016/j.jpba.2015.11.043 ER -
@article{ author = "Foivas, Anargyros and Malenović, Anđelija and Kostić, Nada and Božić, Marija and Knežević, Miroslav and Loukas, Yannis L. and Dotsikas, Yannis", year = "2016", abstract = "In the current study, a rapid and sensitive LC-QTOF-MS/MS method for the determination of brinzolamide in dried blood spots (DBS) was developed and validated. This novel sample collection, storage and transfer technique was suitable for analyzing a drug with high distribution into red blood cells and negligible plasma levels. The method included an isocratic mobile phase consisting of methanol and 10 mM ammonium formate (90:10, v/v) and detection in positive electrospray mode (ESI+). The flow rate was adjusted to 0.350 mL/min yielding retention times of 1.7 min for both brinzolamide and internal standard (IS) rabeprazole on a Cyano analytical column, respectively. The validation of the proposed method over the concentration range 0.500-20.0 mu g/mL was performed in compliance with EMEA and FDA guidelines, assessing all major performance characteristics. Inter- and intra- assay precisions were less than 14%, while inter- and intra- assay accuracies varied from 922 to 111%. No matrix effect was observed and the mean brinzolamide extraction recovery was 93.5%. The method was successfully applied to real DBS samples from patients in steady state condition, receiving brinzolamide ophthalmic suspension 1% (w/v) for several months. Initial concentrations were corrected due to hematocrit effect, using image processing algorithm written in Matlab.", publisher = "Elsevier Science BV, Amsterdam", journal = "Journal of Pharmaceutical and Biomedical Analysis", title = "Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method", volume = "119", pages = "84-90", doi = "10.1016/j.jpba.2015.11.043" }
Foivas, A., Malenović, A., Kostić, N., Božić, M., Knežević, M., Loukas, Y. L.,& Dotsikas, Y.. (2016). Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method. in Journal of Pharmaceutical and Biomedical Analysis Elsevier Science BV, Amsterdam., 119, 84-90. https://doi.org/10.1016/j.jpba.2015.11.043
Foivas A, Malenović A, Kostić N, Božić M, Knežević M, Loukas YL, Dotsikas Y. Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method. in Journal of Pharmaceutical and Biomedical Analysis. 2016;119:84-90. doi:10.1016/j.jpba.2015.11.043 .
Foivas, Anargyros, Malenović, Anđelija, Kostić, Nada, Božić, Marija, Knežević, Miroslav, Loukas, Yannis L., Dotsikas, Yannis, "Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method" in Journal of Pharmaceutical and Biomedical Analysis, 119 (2016):84-90, https://doi.org/10.1016/j.jpba.2015.11.043 . .