Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro
Samo za registrovane korisnike
2017
Autori
Bajić, VladanPotparević, Biljana
Živković, Lada
Pirković, Andrea
Kotur-Stevuljević, Jelena
Isenović, Esma
Sredojević, Dušan
Vukoje, Ivana
Lazić, Vesna
Ahrenkiel, S. Phillip
Nedeljković, Jovan M.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The antigenotoxic and antioxidative properties of surface-modified TiO2 nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO2 NPs). Colloids consisting of the TiO2 NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color. Composition and stability constants of CT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The surface structure of CT complex was determined from infra-red spectra of free and bound AA to the surface Ti atoms. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). The antigenotoxic potential of CT complex was evaluated in leukocytes of whole blood cells in vi...tro by comet assay method. For evaluation of antioxidant properties, total antioxidant status (TAS) and total oxidant status (TOS) were determined in human serum pool in vitro. The presented results indicate that bare TiO2 NPs have more pronounced antigenotoxic effects in comparison with either surface-modified TiO2 NPs with AA or free AA. No significant differences between the antigenotoxic and antioxidative properties of free and bound AA on the TiO2 NPs were noticed in the investigated concentration range. It seems that surface-modified TiO2 NPs with AA and/or similar compounds can be used to maintain its beneficial activities.
Ključne reči:
Ascorbic acid / TiO2 nanoparticles / Charge transfer complex / Antigenotoxic properties / Antioxidative propertiesIzvor:
Colloids and Surfaces B: Biointerfaces, 2017, 155, 323-331Izdavač:
- Elsevier Science BV, Amsterdam
Finansiranje / projekti:
- Materijali redukovane dimenzionalnosti za efikasnu apsorpciju svetlosti i konverziju energije (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45020)
DOI: 10.1016/j.colsurfb.2017.04.032
ISSN: 0927-7765
PubMed: 28448902
WoS: 000403738000038
Scopus: 2-s2.0-85018606012
Institucija/grupa
PharmacyTY - JOUR AU - Bajić, Vladan AU - Potparević, Biljana AU - Živković, Lada AU - Pirković, Andrea AU - Kotur-Stevuljević, Jelena AU - Isenović, Esma AU - Sredojević, Dušan AU - Vukoje, Ivana AU - Lazić, Vesna AU - Ahrenkiel, S. Phillip AU - Nedeljković, Jovan M. PY - 2017 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2968 AB - The antigenotoxic and antioxidative properties of surface-modified TiO2 nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO2 NPs). Colloids consisting of the TiO2 NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color. Composition and stability constants of CT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The surface structure of CT complex was determined from infra-red spectra of free and bound AA to the surface Ti atoms. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). The antigenotoxic potential of CT complex was evaluated in leukocytes of whole blood cells in vitro by comet assay method. For evaluation of antioxidant properties, total antioxidant status (TAS) and total oxidant status (TOS) were determined in human serum pool in vitro. The presented results indicate that bare TiO2 NPs have more pronounced antigenotoxic effects in comparison with either surface-modified TiO2 NPs with AA or free AA. No significant differences between the antigenotoxic and antioxidative properties of free and bound AA on the TiO2 NPs were noticed in the investigated concentration range. It seems that surface-modified TiO2 NPs with AA and/or similar compounds can be used to maintain its beneficial activities. PB - Elsevier Science BV, Amsterdam T2 - Colloids and Surfaces B: Biointerfaces T1 - Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro VL - 155 SP - 323 EP - 331 DO - 10.1016/j.colsurfb.2017.04.032 ER -
@article{ author = "Bajić, Vladan and Potparević, Biljana and Živković, Lada and Pirković, Andrea and Kotur-Stevuljević, Jelena and Isenović, Esma and Sredojević, Dušan and Vukoje, Ivana and Lazić, Vesna and Ahrenkiel, S. Phillip and Nedeljković, Jovan M.", year = "2017", abstract = "The antigenotoxic and antioxidative properties of surface-modified TiO2 nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO2 NPs). Colloids consisting of the TiO2 NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color. Composition and stability constants of CT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The surface structure of CT complex was determined from infra-red spectra of free and bound AA to the surface Ti atoms. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). The antigenotoxic potential of CT complex was evaluated in leukocytes of whole blood cells in vitro by comet assay method. For evaluation of antioxidant properties, total antioxidant status (TAS) and total oxidant status (TOS) were determined in human serum pool in vitro. The presented results indicate that bare TiO2 NPs have more pronounced antigenotoxic effects in comparison with either surface-modified TiO2 NPs with AA or free AA. No significant differences between the antigenotoxic and antioxidative properties of free and bound AA on the TiO2 NPs were noticed in the investigated concentration range. It seems that surface-modified TiO2 NPs with AA and/or similar compounds can be used to maintain its beneficial activities.", publisher = "Elsevier Science BV, Amsterdam", journal = "Colloids and Surfaces B: Biointerfaces", title = "Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro", volume = "155", pages = "323-331", doi = "10.1016/j.colsurfb.2017.04.032" }
Bajić, V., Potparević, B., Živković, L., Pirković, A., Kotur-Stevuljević, J., Isenović, E., Sredojević, D., Vukoje, I., Lazić, V., Ahrenkiel, S. P.,& Nedeljković, J. M.. (2017). Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro. in Colloids and Surfaces B: Biointerfaces Elsevier Science BV, Amsterdam., 155, 323-331. https://doi.org/10.1016/j.colsurfb.2017.04.032
Bajić V, Potparević B, Živković L, Pirković A, Kotur-Stevuljević J, Isenović E, Sredojević D, Vukoje I, Lazić V, Ahrenkiel SP, Nedeljković JM. Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro. in Colloids and Surfaces B: Biointerfaces. 2017;155:323-331. doi:10.1016/j.colsurfb.2017.04.032 .
Bajić, Vladan, Potparević, Biljana, Živković, Lada, Pirković, Andrea, Kotur-Stevuljević, Jelena, Isenović, Esma, Sredojević, Dušan, Vukoje, Ivana, Lazić, Vesna, Ahrenkiel, S. Phillip, Nedeljković, Jovan M., "Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro" in Colloids and Surfaces B: Biointerfaces, 155 (2017):323-331, https://doi.org/10.1016/j.colsurfb.2017.04.032 . .