dc.creator | Albert, Lea | |
dc.creator | Penalver, Alberto | |
dc.creator | Đoković, Nemanja | |
dc.creator | Werel, Laura | |
dc.creator | Hoffarth, Malte | |
dc.creator | Ružić, Dušan | |
dc.creator | Xu, Jing | |
dc.creator | Essen, Lars-Oliver | |
dc.creator | Nikolić, Katarina | |
dc.creator | Dou, Yali | |
dc.creator | Vazquez, Olalla | |
dc.date.accessioned | 2019-09-02T12:08:54Z | |
dc.date.available | 2019-09-02T12:08:54Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 1439-4227 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/3258 | |
dc.description.abstract | Life relies on a myriad of carefully orchestrated processes, in which proteins and their direct interplay ultimately determine cellular function and disease. Modulation of this complex crosstalk has recently attracted attention, even as a novel therapeutic strategy. Herein, we describe the synthesis and characterization of two visible-light-responsive peptide backbone photoswitches based on azobenzene derivatives, to exert optical control over protein-protein interactions (PPI). The novel peptidomimetics undergo fast and reversible isomerization with low photochemical fatigue under alternatively blue-/green-light irradiation cycles. Both bind in the nanomolar range to the protein of interest. Importantly, the best peptidomimetic displays a clear difference between isomers in its protein-binding capacity and, in turn, in its potential to inhibit enzymatic activity through PPI disruption. In addition, crystal structure determination, docking and molecular dynamics calculations allow a molecular interpretation and open up new avenues in the design and synthesis of future photoswitchable PPI modulators. | en |
dc.publisher | Wiley-VCH Verlag GMBH, Weinheim | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/171017/RS// | |
dc.rights | restrictedAccess | |
dc.source | Chembiochem | |
dc.subject | azobenzenes | en |
dc.subject | isomerization | en |
dc.subject | peptidomimetics | en |
dc.subject | photochemistry | en |
dc.subject | protein-protein interactions | en |
dc.title | Modulating Protein-Protein Interactions with Visible-Light-Responsive Peptide Backbone Photoswitches | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Aлберт, Леа; Ружић, Душан; Николић, Катарина; Xу, Јинг; Хоффартх, Малте; Вазqуез, Олалла; Wерел, Лаура; Пеналвер, Aлберто; Ђоковић, Немања; Ессен, Ларс-Оливер; Доу, Yали; | |
dc.citation.volume | 20 | |
dc.citation.issue | 11 | |
dc.citation.spage | 1417 | |
dc.citation.epage | 1429 | |
dc.citation.other | 20(11): 1417-1429 | |
dc.citation.rank | M22 | |
dc.identifier.wos | 000474071700012 | |
dc.identifier.doi | 10.1002/cbic.201800737 | |
dc.identifier.pmid | 30675988 | |
dc.identifier.scopus | 2-s2.0-85065047737 | |
dc.type.version | publishedVersion | |