Superoxide dismutase isoenzymes gene expression in peripheral blood mononuclear cells in patients with coronary artery disease
2019
Аутори
Ninić, AnaBogavac-Stanojević, Nataša
Sopić, Miron
Munjas, Jelena
Kotur-Stevuljević, Jelena
Miljković, Milica
Gojković, Tamara
Kalimanovska-Oštrić, Dimitra
Spasojević-Kalimanovska, Vesna
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background: Coronary artery disease (CAD) is one of the most important causes of mortality and morbidity in wide world population. Dyslipidemia, inflammation and oxidative stress may contribute to disruption of endothelium structure and function, atherosclerosis and CAD. Our study was aimed to determine whether Cu/Zn superoxide dismutase (Cu/Zn SOD) and Mn superoxide dismutase (Mn SOD) gene expression could be modulated by oxidative stress in CAD patients. Methods: This study included 77 CAD patients and 31 apparently healthy persons. Serum lipid levels, high sensitivity C-reactive protein (hsCRP), total antioxidant status (TAS) and thiobarbituric acid-reacting substances (TBARS) were measured. SOD isoenzymes gene expression was determined in peripheral blood mononuclear cells using quantitative polymerase chain reaction. Results: Mn SOD messenger ribonucleic acid (mRNA) levels were significantly lower in CAD patients than in controls (p=0.011), while Cu/Zn SOD mRNA levels did not chan...ge significantly between tested groups (p=0.091). We found significantly lower high-density lipoprotein-cholesterol (HDL-c) (p lt 0.001) and TAS (p lt 0.001) levels and significantly higher hsCRP (p=0.002) and TBARS (p lt 0.001) in CAD patients than in controls. There were significant positive correlations between TAS and Mn SOD mRNA (rho=0.243, p=0.020) and TAS and Cu/Zn SOD mRNA (rho=0.359, p lt 0.001). TBARS negatively correlated only with Cu/Zn SOD mRNA (rho=-0.215, p=0.040). TAS levels remained independent predictor for Mn SOD mRNA levels (OR = 2.995, p=0.034). Conclusions: Results of this study showed that Mn SOD gene expression were decreased in CAD patients compared to controls and can be modulated by nonenzymatic antioxidant status in blood.
Кључне речи:
CAD / oxidative stress / Cu/Zn SOD / Mn SOD / qPCRИзвор:
Journal of Medical Biochemistry, 2019, 38, 3, 284-291Издавач:
- Društvo medicinskih biohemičara Srbije, Beograd i Versita
Финансирање / пројекти:
DOI: 10.2478/jomb-2018-0041
ISSN: 1452-8258
PubMed: 31156338
WoS: 000468363100005
Scopus: 2-s2.0-85057522008
Институција/група
PharmacyTY - JOUR AU - Ninić, Ana AU - Bogavac-Stanojević, Nataša AU - Sopić, Miron AU - Munjas, Jelena AU - Kotur-Stevuljević, Jelena AU - Miljković, Milica AU - Gojković, Tamara AU - Kalimanovska-Oštrić, Dimitra AU - Spasojević-Kalimanovska, Vesna PY - 2019 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3339 AB - Background: Coronary artery disease (CAD) is one of the most important causes of mortality and morbidity in wide world population. Dyslipidemia, inflammation and oxidative stress may contribute to disruption of endothelium structure and function, atherosclerosis and CAD. Our study was aimed to determine whether Cu/Zn superoxide dismutase (Cu/Zn SOD) and Mn superoxide dismutase (Mn SOD) gene expression could be modulated by oxidative stress in CAD patients. Methods: This study included 77 CAD patients and 31 apparently healthy persons. Serum lipid levels, high sensitivity C-reactive protein (hsCRP), total antioxidant status (TAS) and thiobarbituric acid-reacting substances (TBARS) were measured. SOD isoenzymes gene expression was determined in peripheral blood mononuclear cells using quantitative polymerase chain reaction. Results: Mn SOD messenger ribonucleic acid (mRNA) levels were significantly lower in CAD patients than in controls (p=0.011), while Cu/Zn SOD mRNA levels did not change significantly between tested groups (p=0.091). We found significantly lower high-density lipoprotein-cholesterol (HDL-c) (p lt 0.001) and TAS (p lt 0.001) levels and significantly higher hsCRP (p=0.002) and TBARS (p lt 0.001) in CAD patients than in controls. There were significant positive correlations between TAS and Mn SOD mRNA (rho=0.243, p=0.020) and TAS and Cu/Zn SOD mRNA (rho=0.359, p lt 0.001). TBARS negatively correlated only with Cu/Zn SOD mRNA (rho=-0.215, p=0.040). TAS levels remained independent predictor for Mn SOD mRNA levels (OR = 2.995, p=0.034). Conclusions: Results of this study showed that Mn SOD gene expression were decreased in CAD patients compared to controls and can be modulated by nonenzymatic antioxidant status in blood. PB - Društvo medicinskih biohemičara Srbije, Beograd i Versita T2 - Journal of Medical Biochemistry T1 - Superoxide dismutase isoenzymes gene expression in peripheral blood mononuclear cells in patients with coronary artery disease VL - 38 IS - 3 SP - 284 EP - 291 DO - 10.2478/jomb-2018-0041 ER -
@article{ author = "Ninić, Ana and Bogavac-Stanojević, Nataša and Sopić, Miron and Munjas, Jelena and Kotur-Stevuljević, Jelena and Miljković, Milica and Gojković, Tamara and Kalimanovska-Oštrić, Dimitra and Spasojević-Kalimanovska, Vesna", year = "2019", abstract = "Background: Coronary artery disease (CAD) is one of the most important causes of mortality and morbidity in wide world population. Dyslipidemia, inflammation and oxidative stress may contribute to disruption of endothelium structure and function, atherosclerosis and CAD. Our study was aimed to determine whether Cu/Zn superoxide dismutase (Cu/Zn SOD) and Mn superoxide dismutase (Mn SOD) gene expression could be modulated by oxidative stress in CAD patients. Methods: This study included 77 CAD patients and 31 apparently healthy persons. Serum lipid levels, high sensitivity C-reactive protein (hsCRP), total antioxidant status (TAS) and thiobarbituric acid-reacting substances (TBARS) were measured. SOD isoenzymes gene expression was determined in peripheral blood mononuclear cells using quantitative polymerase chain reaction. Results: Mn SOD messenger ribonucleic acid (mRNA) levels were significantly lower in CAD patients than in controls (p=0.011), while Cu/Zn SOD mRNA levels did not change significantly between tested groups (p=0.091). We found significantly lower high-density lipoprotein-cholesterol (HDL-c) (p lt 0.001) and TAS (p lt 0.001) levels and significantly higher hsCRP (p=0.002) and TBARS (p lt 0.001) in CAD patients than in controls. There were significant positive correlations between TAS and Mn SOD mRNA (rho=0.243, p=0.020) and TAS and Cu/Zn SOD mRNA (rho=0.359, p lt 0.001). TBARS negatively correlated only with Cu/Zn SOD mRNA (rho=-0.215, p=0.040). TAS levels remained independent predictor for Mn SOD mRNA levels (OR = 2.995, p=0.034). Conclusions: Results of this study showed that Mn SOD gene expression were decreased in CAD patients compared to controls and can be modulated by nonenzymatic antioxidant status in blood.", publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita", journal = "Journal of Medical Biochemistry", title = "Superoxide dismutase isoenzymes gene expression in peripheral blood mononuclear cells in patients with coronary artery disease", volume = "38", number = "3", pages = "284-291", doi = "10.2478/jomb-2018-0041" }
Ninić, A., Bogavac-Stanojević, N., Sopić, M., Munjas, J., Kotur-Stevuljević, J., Miljković, M., Gojković, T., Kalimanovska-Oštrić, D.,& Spasojević-Kalimanovska, V.. (2019). Superoxide dismutase isoenzymes gene expression in peripheral blood mononuclear cells in patients with coronary artery disease. in Journal of Medical Biochemistry Društvo medicinskih biohemičara Srbije, Beograd i Versita., 38(3), 284-291. https://doi.org/10.2478/jomb-2018-0041
Ninić A, Bogavac-Stanojević N, Sopić M, Munjas J, Kotur-Stevuljević J, Miljković M, Gojković T, Kalimanovska-Oštrić D, Spasojević-Kalimanovska V. Superoxide dismutase isoenzymes gene expression in peripheral blood mononuclear cells in patients with coronary artery disease. in Journal of Medical Biochemistry. 2019;38(3):284-291. doi:10.2478/jomb-2018-0041 .
Ninić, Ana, Bogavac-Stanojević, Nataša, Sopić, Miron, Munjas, Jelena, Kotur-Stevuljević, Jelena, Miljković, Milica, Gojković, Tamara, Kalimanovska-Oštrić, Dimitra, Spasojević-Kalimanovska, Vesna, "Superoxide dismutase isoenzymes gene expression in peripheral blood mononuclear cells in patients with coronary artery disease" in Journal of Medical Biochemistry, 38, no. 3 (2019):284-291, https://doi.org/10.2478/jomb-2018-0041 . .