Fucus spiralis extract and fractions: Anticancer and pharmacological potentials
Аутори
Grozdanić, NađaĐuričić, Ivana
Kosanić, Marijana
Zdunić, Gordana
Šavikin, Katarina
Etahiri, Samira
Assobhei, Omar
Benba, Jamila
Petović, Slavica
Matić, Ivana Z.
Stanojković, Tatjana P.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Purpose: Sea macroalgae are an important source of biologically highly valuable compounds. The main aim of this study was to investigate the in vitro anticancer properties and chemical composition of the dichloromethane-methanol extract and three fractions of the Fucus spiralis from coastline of Morocco. Methods: Fractions were made from dichloromethane: methanol (1:1) extract of Fucus spiralis: petroleum-ether, ethyl-acetate and n-butanol. Extract and fractions were screened for in vitro cytotoxicity by MTT assay against human cervical adenocarcinoma (HeLa), colorectal adenocarcinoma (LS-174T), lung carcinoma (A549), and normal human lung fibroblasts (MRC-5). Cell cycle distribution of the HeLa cells was evaluated using flow cytometry. Acridine orange (AO)-ethidium bromide (EB) staining was used to assess morphological changes of HeLa cells under fluorescence microscope. Anti-migration and anti-angiogenic properties were investigated using scratch and tube formation assays against hum...an endothelium-derived permanent EA.hy926 cell line. Antidiabetic activity was tested using anti-α-glucosidase assay. Antimicrobial effect was tested using micro- dilution method. Results: Petroleum-ether fraction оf Fucus spiralis rich in fatty acids exerted the highest cytotoxicity against HeLa cells. Ethyl-acetate and petroleum-ether fractions induced the highest accumulation of the HeLa cells in sub-G1 and G2/M phases. Extract and fractions showed proapoptotic effect on HeLa cells under fluorescent microscope. They exhibited antimigratory and antiangiogenic effects in vitro. IC50 value for α-glucosidase inhibitory activity was much stronger than standard acarbose. n-Butanol fraction exerted the highest antibacterial and antifungal activity. Conclusions: The investigation of various biological activities of the extract and fractions obtained from Fucus spiralis may suggest a promising anticancer and pharmacological potential of this edible macroalga.
Кључне речи:
Cancer / Cancer cells / Cytotoxic / Fatty acids / Fucus spiralis / In vitroИзвор:
Journal of B.U.ON., 2020, 25, 2, 1219-1229Издавач:
- Zerbinis Publications
Финансирање / пројекти:
- Модификатори биолошког одговора у физиолошким и патолошким стањима (RS-MESTD-Basic Research (BR or ON)-175011)
- Традиционални и нови производи од плодова гајених и самониклих врста воћака и винове лозе и нус-продуката у преради, са посебним освртом на аутохтоне сорте: хемијска карактеризација и биолошки профил (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46013)
- Карактеризација и примена метаболита гљива и утврђивање потенцијала нових биофунгицида (RS-MESTD-Basic Research (BR or ON)-173032)
- Развој и примена нових и традиционалних технологија у производњи конкурентних прехрамбених производа са додатом вредношћу за европско и светско тржиште - Створимо богатство из богатства Србије (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46001)
- Ministry of Science of the Republic of Montenegro.
Институција/група
PharmacyTY - JOUR AU - Grozdanić, Nađa AU - Đuričić, Ivana AU - Kosanić, Marijana AU - Zdunić, Gordana AU - Šavikin, Katarina AU - Etahiri, Samira AU - Assobhei, Omar AU - Benba, Jamila AU - Petović, Slavica AU - Matić, Ivana Z. AU - Stanojković, Tatjana P. PY - 2020 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3605 AB - Purpose: Sea macroalgae are an important source of biologically highly valuable compounds. The main aim of this study was to investigate the in vitro anticancer properties and chemical composition of the dichloromethane-methanol extract and three fractions of the Fucus spiralis from coastline of Morocco. Methods: Fractions were made from dichloromethane: methanol (1:1) extract of Fucus spiralis: petroleum-ether, ethyl-acetate and n-butanol. Extract and fractions were screened for in vitro cytotoxicity by MTT assay against human cervical adenocarcinoma (HeLa), colorectal adenocarcinoma (LS-174T), lung carcinoma (A549), and normal human lung fibroblasts (MRC-5). Cell cycle distribution of the HeLa cells was evaluated using flow cytometry. Acridine orange (AO)-ethidium bromide (EB) staining was used to assess morphological changes of HeLa cells under fluorescence microscope. Anti-migration and anti-angiogenic properties were investigated using scratch and tube formation assays against human endothelium-derived permanent EA.hy926 cell line. Antidiabetic activity was tested using anti-α-glucosidase assay. Antimicrobial effect was tested using micro- dilution method. Results: Petroleum-ether fraction оf Fucus spiralis rich in fatty acids exerted the highest cytotoxicity against HeLa cells. Ethyl-acetate and petroleum-ether fractions induced the highest accumulation of the HeLa cells in sub-G1 and G2/M phases. Extract and fractions showed proapoptotic effect on HeLa cells under fluorescent microscope. They exhibited antimigratory and antiangiogenic effects in vitro. IC50 value for α-glucosidase inhibitory activity was much stronger than standard acarbose. n-Butanol fraction exerted the highest antibacterial and antifungal activity. Conclusions: The investigation of various biological activities of the extract and fractions obtained from Fucus spiralis may suggest a promising anticancer and pharmacological potential of this edible macroalga. PB - Zerbinis Publications T2 - Journal of B.U.ON. T1 - Fucus spiralis extract and fractions: Anticancer and pharmacological potentials VL - 25 IS - 2 SP - 1219 EP - 1229 UR - https://hdl.handle.net/21.15107/rcub_farfar_3605 ER -
@article{ author = "Grozdanić, Nađa and Đuričić, Ivana and Kosanić, Marijana and Zdunić, Gordana and Šavikin, Katarina and Etahiri, Samira and Assobhei, Omar and Benba, Jamila and Petović, Slavica and Matić, Ivana Z. and Stanojković, Tatjana P.", year = "2020", abstract = "Purpose: Sea macroalgae are an important source of biologically highly valuable compounds. The main aim of this study was to investigate the in vitro anticancer properties and chemical composition of the dichloromethane-methanol extract and three fractions of the Fucus spiralis from coastline of Morocco. Methods: Fractions were made from dichloromethane: methanol (1:1) extract of Fucus spiralis: petroleum-ether, ethyl-acetate and n-butanol. Extract and fractions were screened for in vitro cytotoxicity by MTT assay against human cervical adenocarcinoma (HeLa), colorectal adenocarcinoma (LS-174T), lung carcinoma (A549), and normal human lung fibroblasts (MRC-5). Cell cycle distribution of the HeLa cells was evaluated using flow cytometry. Acridine orange (AO)-ethidium bromide (EB) staining was used to assess morphological changes of HeLa cells under fluorescence microscope. Anti-migration and anti-angiogenic properties were investigated using scratch and tube formation assays against human endothelium-derived permanent EA.hy926 cell line. Antidiabetic activity was tested using anti-α-glucosidase assay. Antimicrobial effect was tested using micro- dilution method. Results: Petroleum-ether fraction оf Fucus spiralis rich in fatty acids exerted the highest cytotoxicity against HeLa cells. Ethyl-acetate and petroleum-ether fractions induced the highest accumulation of the HeLa cells in sub-G1 and G2/M phases. Extract and fractions showed proapoptotic effect on HeLa cells under fluorescent microscope. They exhibited antimigratory and antiangiogenic effects in vitro. IC50 value for α-glucosidase inhibitory activity was much stronger than standard acarbose. n-Butanol fraction exerted the highest antibacterial and antifungal activity. Conclusions: The investigation of various biological activities of the extract and fractions obtained from Fucus spiralis may suggest a promising anticancer and pharmacological potential of this edible macroalga.", publisher = "Zerbinis Publications", journal = "Journal of B.U.ON.", title = "Fucus spiralis extract and fractions: Anticancer and pharmacological potentials", volume = "25", number = "2", pages = "1219-1229", url = "https://hdl.handle.net/21.15107/rcub_farfar_3605" }
Grozdanić, N., Đuričić, I., Kosanić, M., Zdunić, G., Šavikin, K., Etahiri, S., Assobhei, O., Benba, J., Petović, S., Matić, I. Z.,& Stanojković, T. P.. (2020). Fucus spiralis extract and fractions: Anticancer and pharmacological potentials. in Journal of B.U.ON. Zerbinis Publications., 25(2), 1219-1229. https://hdl.handle.net/21.15107/rcub_farfar_3605
Grozdanić N, Đuričić I, Kosanić M, Zdunić G, Šavikin K, Etahiri S, Assobhei O, Benba J, Petović S, Matić IZ, Stanojković TP. Fucus spiralis extract and fractions: Anticancer and pharmacological potentials. in Journal of B.U.ON.. 2020;25(2):1219-1229. https://hdl.handle.net/21.15107/rcub_farfar_3605 .
Grozdanić, Nađa, Đuričić, Ivana, Kosanić, Marijana, Zdunić, Gordana, Šavikin, Katarina, Etahiri, Samira, Assobhei, Omar, Benba, Jamila, Petović, Slavica, Matić, Ivana Z., Stanojković, Tatjana P., "Fucus spiralis extract and fractions: Anticancer and pharmacological potentials" in Journal of B.U.ON., 25, no. 2 (2020):1219-1229, https://hdl.handle.net/21.15107/rcub_farfar_3605 .