Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts
Аутори
Kozarević Cilović, EminaDautović, Esmeralda
Halilčević, Dalila
Softić, Adaleta
Srabović, Nahida
Šarić-Kundalić, Broza
Delić, Nina
Kolarević, Lamija
Mekić, Lejla
Ibišević, Merima
Horozić, Emir
Arsenijević, Jelena
Maksimović, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Aims: The aims of the study were to analyse the polyphenols of Pulicaria dysenterica (L.) Bernh. methanolic extracts from aerial and underground parts, assessment of antioxidant activity and to evaluate their cytotoxicity on HeLa cells of cervical cancer.
Methodology: The total phenolic content (TPC) of extracts was determined by the Folin-Ciocalteu spectrophotometric method. The qualitative and quantitative analysis of individual polyphenolic compounds were performed by the reverse phase HPLC method. The antioxidant capacity was evaluated by both, 2,2-diphenyl1-picrylhydrazyl radical and FRAP assay, while cytotoxicity of the extracts was assessed by MTT assay.
Results: TPC of the samples were 127.62±2.22 and 244.12±8.84 mg gallic acid equivalent/g extract. In the extracts chlorogenic acid in amount of 10.06±0.96 and 11.32±0.28 mg/g, flavonoid rutin in amount of 5.68±0.13 mg/g and three caffeic acid derivatives were recorded. Extract from underground parts achieved better antioxi...dant activity with IC50 value 55.36±0.75 µg/mL and FRAP value 2411.12±37.22 µmol Fe2+g-1 compared to the one from aerial parts. Extract from aerial parts achieved better cytotoxic activity with 50% inhibition of viability (IC50) at concentration of 0.389±0.07 mg/mL, against HeLa cells, compared to the extract from underground parts.
Conclusion: Analyzed Pulicaria dysenterica extracts contained phenolic acids and flavonoids. The extracts showed good antioxidant activity and cytotoxic properties against HeLa cells in vitro.
Кључне речи:
Pulicaria dysenterica / polyphenols / antioxidants / cytotoxicityИзвор:
International Research Journal of Pure and Applied Chemistry, 2022, 23, 5, 23-32Издавач:
- International Research Journal of Pure and Applied Chemistry
Финансирање / пројекти:
- This research is funded by the Federal Ministry of Education and Science (FB&H)
Институција/група
PharmacyTY - JOUR AU - Kozarević Cilović, Emina AU - Dautović, Esmeralda AU - Halilčević, Dalila AU - Softić, Adaleta AU - Srabović, Nahida AU - Šarić-Kundalić, Broza AU - Delić, Nina AU - Kolarević, Lamija AU - Mekić, Lejla AU - Ibišević, Merima AU - Horozić, Emir AU - Arsenijević, Jelena AU - Maksimović, Zoran PY - 2022 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4624 AB - Aims: The aims of the study were to analyse the polyphenols of Pulicaria dysenterica (L.) Bernh. methanolic extracts from aerial and underground parts, assessment of antioxidant activity and to evaluate their cytotoxicity on HeLa cells of cervical cancer. Methodology: The total phenolic content (TPC) of extracts was determined by the Folin-Ciocalteu spectrophotometric method. The qualitative and quantitative analysis of individual polyphenolic compounds were performed by the reverse phase HPLC method. The antioxidant capacity was evaluated by both, 2,2-diphenyl1-picrylhydrazyl radical and FRAP assay, while cytotoxicity of the extracts was assessed by MTT assay. Results: TPC of the samples were 127.62±2.22 and 244.12±8.84 mg gallic acid equivalent/g extract. In the extracts chlorogenic acid in amount of 10.06±0.96 and 11.32±0.28 mg/g, flavonoid rutin in amount of 5.68±0.13 mg/g and three caffeic acid derivatives were recorded. Extract from underground parts achieved better antioxidant activity with IC50 value 55.36±0.75 µg/mL and FRAP value 2411.12±37.22 µmol Fe2+g-1 compared to the one from aerial parts. Extract from aerial parts achieved better cytotoxic activity with 50% inhibition of viability (IC50) at concentration of 0.389±0.07 mg/mL, against HeLa cells, compared to the extract from underground parts. Conclusion: Analyzed Pulicaria dysenterica extracts contained phenolic acids and flavonoids. The extracts showed good antioxidant activity and cytotoxic properties against HeLa cells in vitro. PB - International Research Journal of Pure and Applied Chemistry T2 - International Research Journal of Pure and Applied Chemistry T1 - Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts VL - 23 IS - 5 SP - 23 EP - 32 DO - 10.9734/irjpac/2022/v23i5788 ER -
@article{ author = "Kozarević Cilović, Emina and Dautović, Esmeralda and Halilčević, Dalila and Softić, Adaleta and Srabović, Nahida and Šarić-Kundalić, Broza and Delić, Nina and Kolarević, Lamija and Mekić, Lejla and Ibišević, Merima and Horozić, Emir and Arsenijević, Jelena and Maksimović, Zoran", year = "2022", abstract = "Aims: The aims of the study were to analyse the polyphenols of Pulicaria dysenterica (L.) Bernh. methanolic extracts from aerial and underground parts, assessment of antioxidant activity and to evaluate their cytotoxicity on HeLa cells of cervical cancer. Methodology: The total phenolic content (TPC) of extracts was determined by the Folin-Ciocalteu spectrophotometric method. The qualitative and quantitative analysis of individual polyphenolic compounds were performed by the reverse phase HPLC method. The antioxidant capacity was evaluated by both, 2,2-diphenyl1-picrylhydrazyl radical and FRAP assay, while cytotoxicity of the extracts was assessed by MTT assay. Results: TPC of the samples were 127.62±2.22 and 244.12±8.84 mg gallic acid equivalent/g extract. In the extracts chlorogenic acid in amount of 10.06±0.96 and 11.32±0.28 mg/g, flavonoid rutin in amount of 5.68±0.13 mg/g and three caffeic acid derivatives were recorded. Extract from underground parts achieved better antioxidant activity with IC50 value 55.36±0.75 µg/mL and FRAP value 2411.12±37.22 µmol Fe2+g-1 compared to the one from aerial parts. Extract from aerial parts achieved better cytotoxic activity with 50% inhibition of viability (IC50) at concentration of 0.389±0.07 mg/mL, against HeLa cells, compared to the extract from underground parts. Conclusion: Analyzed Pulicaria dysenterica extracts contained phenolic acids and flavonoids. The extracts showed good antioxidant activity and cytotoxic properties against HeLa cells in vitro.", publisher = "International Research Journal of Pure and Applied Chemistry", journal = "International Research Journal of Pure and Applied Chemistry", title = "Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts", volume = "23", number = "5", pages = "23-32", doi = "10.9734/irjpac/2022/v23i5788" }
Kozarević Cilović, E., Dautović, E., Halilčević, D., Softić, A., Srabović, N., Šarić-Kundalić, B., Delić, N., Kolarević, L., Mekić, L., Ibišević, M., Horozić, E., Arsenijević, J.,& Maksimović, Z.. (2022). Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts. in International Research Journal of Pure and Applied Chemistry International Research Journal of Pure and Applied Chemistry., 23(5), 23-32. https://doi.org/10.9734/irjpac/2022/v23i5788
Kozarević Cilović E, Dautović E, Halilčević D, Softić A, Srabović N, Šarić-Kundalić B, Delić N, Kolarević L, Mekić L, Ibišević M, Horozić E, Arsenijević J, Maksimović Z. Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts. in International Research Journal of Pure and Applied Chemistry. 2022;23(5):23-32. doi:10.9734/irjpac/2022/v23i5788 .
Kozarević Cilović, Emina, Dautović, Esmeralda, Halilčević, Dalila, Softić, Adaleta, Srabović, Nahida, Šarić-Kundalić, Broza, Delić, Nina, Kolarević, Lamija, Mekić, Lejla, Ibišević, Merima, Horozić, Emir, Arsenijević, Jelena, Maksimović, Zoran, "Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts" in International Research Journal of Pure and Applied Chemistry, 23, no. 5 (2022):23-32, https://doi.org/10.9734/irjpac/2022/v23i5788 . .