DPPH radical scavenging potential of the root essential oils of five Heracleum L. taxa
Апстракт
In the Balkan Peninsula, Heracleum L. taxa (Apiaceae) were traditionally used for the treatment of various digestive and respiratory diseases, epilepsy, hypertension and sexual weakness. The purpose of this work was to investigate 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability of the root essential oils of some of the Balkan Heracleum taxa, i.e. H. sibiricum L., H. ternatum Velen., H. verticillatum Pančić, H. pyrenaicum subsp. pollinianum (Bertol.) F. Pedrotti & Pignatti and H. pyrenaicum subsp. orsinii (Guss.) F. Pedrotti & Pignatti. GC-FID and GC-MS analysis of these oils, obtained by hydrodistillation using Clevenger-type apparatus, revealed the domination of monoterpenes, mostly β-pinene (26.2-47.3%). Additionally, H. sibiricum root oil was rich in phenylpropanoids, mainly elemicin (25.6%) and methyl eugenol (22.3%). In colorimetric DPPH assay, the strongest activity was exhibited by H. sibiricum oil (SC50=5.19 μL/mL), followed by H. pyrenaicum subsp. orsinii, H. t...ernatum, H. pyrenaicum subsp. pollinianum and H. verticillatum oils (SC50=7.85-12.33 μL/mL). In TLC-DPPH (dot-blot) test, three the most active root oils, i.e. those of H. sibiricum, H. ternatum and H. pyrenaicum subsp. orsinii revealed yellow
anti-DPPH zones (Rf=0.30-0.42), which were then eluted and analyzed by GC-FID and GC-MS. It was shown that elemicin and methyl eugenol, dominant in H. sibiricum oil, were also the most abundant in its anti-DPPH zone (64.5 and 19.5%). β-Pinene and other monoterpene hydrocarbons were not detected in this, and also in the active zones of H. ternatum and H. pyrenaicum subsp. orsinii oils (both contained two closely located anti-DPPH zones, which were eluted together). Anti-DPPH zones of H. ternatum oil were dominated by trans-sabinol (21.8%), spathulenol (21.7%) and (E)-sesquilavandulol (13.1%), and those of H. pyrenaicum subsp. orsinii oil by (E)-sesquilavandulol (14.5%) and intermedeol (13.6%). These oxygenated terpenes were detected in H. ternatum and H. pyrenaicum subsp. orsinii oils only in small quantities. Another minor constituent of H. pyrenaicum subsp. orsinii root oil, (Z)-falcarinol, was among the dominant ones in its anti-DPPH zones (11.6%).
Кључне речи:
Heracleum taxa / root essential oils / GC-FID and GC-MS / DPPH radical / phenylpropanoids / oxygenated terpenesИзвор:
Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 2018, 42, 1 supplement, 131-131Издавач:
- Institute of Botany and Botanical Garden Jevremovac, Belgrade
Финансирање / пројекти:
- Испитивање лековитог потенцијала биљака: морфолошка, хемијска и фармаколошка карактеризација (RS-MESTD-Basic Research (BR or ON)-173021)
Напомена:
- 7th Balkan Botanical Congress, 10-14th September 2018, Novi Sad, Serbia
- Saopštenje sa međunarodnog skupa štampano u izvodu
Институција/група
PharmacyTY - CONF AU - Ušjak, Ljuboš AU - Drobac, Milica AU - Niketić, Marjan AU - Petrović, Silvana PY - 2018 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5243 AB - In the Balkan Peninsula, Heracleum L. taxa (Apiaceae) were traditionally used for the treatment of various digestive and respiratory diseases, epilepsy, hypertension and sexual weakness. The purpose of this work was to investigate 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability of the root essential oils of some of the Balkan Heracleum taxa, i.e. H. sibiricum L., H. ternatum Velen., H. verticillatum Pančić, H. pyrenaicum subsp. pollinianum (Bertol.) F. Pedrotti & Pignatti and H. pyrenaicum subsp. orsinii (Guss.) F. Pedrotti & Pignatti. GC-FID and GC-MS analysis of these oils, obtained by hydrodistillation using Clevenger-type apparatus, revealed the domination of monoterpenes, mostly β-pinene (26.2-47.3%). Additionally, H. sibiricum root oil was rich in phenylpropanoids, mainly elemicin (25.6%) and methyl eugenol (22.3%). In colorimetric DPPH assay, the strongest activity was exhibited by H. sibiricum oil (SC50=5.19 μL/mL), followed by H. pyrenaicum subsp. orsinii, H. ternatum, H. pyrenaicum subsp. pollinianum and H. verticillatum oils (SC50=7.85-12.33 μL/mL). In TLC-DPPH (dot-blot) test, three the most active root oils, i.e. those of H. sibiricum, H. ternatum and H. pyrenaicum subsp. orsinii revealed yellow anti-DPPH zones (Rf=0.30-0.42), which were then eluted and analyzed by GC-FID and GC-MS. It was shown that elemicin and methyl eugenol, dominant in H. sibiricum oil, were also the most abundant in its anti-DPPH zone (64.5 and 19.5%). β-Pinene and other monoterpene hydrocarbons were not detected in this, and also in the active zones of H. ternatum and H. pyrenaicum subsp. orsinii oils (both contained two closely located anti-DPPH zones, which were eluted together). Anti-DPPH zones of H. ternatum oil were dominated by trans-sabinol (21.8%), spathulenol (21.7%) and (E)-sesquilavandulol (13.1%), and those of H. pyrenaicum subsp. orsinii oil by (E)-sesquilavandulol (14.5%) and intermedeol (13.6%). These oxygenated terpenes were detected in H. ternatum and H. pyrenaicum subsp. orsinii oils only in small quantities. Another minor constituent of H. pyrenaicum subsp. orsinii root oil, (Z)-falcarinol, was among the dominant ones in its anti-DPPH zones (11.6%). PB - Institute of Botany and Botanical Garden Jevremovac, Belgrade C3 - Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts T1 - DPPH radical scavenging potential of the root essential oils of five Heracleum L. taxa VL - 42 IS - 1 supplement SP - 131 EP - 131 UR - https://hdl.handle.net/21.15107/rcub_farfar_5243 ER -
@conference{ author = "Ušjak, Ljuboš and Drobac, Milica and Niketić, Marjan and Petrović, Silvana", year = "2018", abstract = "In the Balkan Peninsula, Heracleum L. taxa (Apiaceae) were traditionally used for the treatment of various digestive and respiratory diseases, epilepsy, hypertension and sexual weakness. The purpose of this work was to investigate 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability of the root essential oils of some of the Balkan Heracleum taxa, i.e. H. sibiricum L., H. ternatum Velen., H. verticillatum Pančić, H. pyrenaicum subsp. pollinianum (Bertol.) F. Pedrotti & Pignatti and H. pyrenaicum subsp. orsinii (Guss.) F. Pedrotti & Pignatti. GC-FID and GC-MS analysis of these oils, obtained by hydrodistillation using Clevenger-type apparatus, revealed the domination of monoterpenes, mostly β-pinene (26.2-47.3%). Additionally, H. sibiricum root oil was rich in phenylpropanoids, mainly elemicin (25.6%) and methyl eugenol (22.3%). In colorimetric DPPH assay, the strongest activity was exhibited by H. sibiricum oil (SC50=5.19 μL/mL), followed by H. pyrenaicum subsp. orsinii, H. ternatum, H. pyrenaicum subsp. pollinianum and H. verticillatum oils (SC50=7.85-12.33 μL/mL). In TLC-DPPH (dot-blot) test, three the most active root oils, i.e. those of H. sibiricum, H. ternatum and H. pyrenaicum subsp. orsinii revealed yellow anti-DPPH zones (Rf=0.30-0.42), which were then eluted and analyzed by GC-FID and GC-MS. It was shown that elemicin and methyl eugenol, dominant in H. sibiricum oil, were also the most abundant in its anti-DPPH zone (64.5 and 19.5%). β-Pinene and other monoterpene hydrocarbons were not detected in this, and also in the active zones of H. ternatum and H. pyrenaicum subsp. orsinii oils (both contained two closely located anti-DPPH zones, which were eluted together). Anti-DPPH zones of H. ternatum oil were dominated by trans-sabinol (21.8%), spathulenol (21.7%) and (E)-sesquilavandulol (13.1%), and those of H. pyrenaicum subsp. orsinii oil by (E)-sesquilavandulol (14.5%) and intermedeol (13.6%). These oxygenated terpenes were detected in H. ternatum and H. pyrenaicum subsp. orsinii oils only in small quantities. Another minor constituent of H. pyrenaicum subsp. orsinii root oil, (Z)-falcarinol, was among the dominant ones in its anti-DPPH zones (11.6%).", publisher = "Institute of Botany and Botanical Garden Jevremovac, Belgrade", journal = "Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts", title = "DPPH radical scavenging potential of the root essential oils of five Heracleum L. taxa", volume = "42", number = "1 supplement", pages = "131-131", url = "https://hdl.handle.net/21.15107/rcub_farfar_5243" }
Ušjak, L., Drobac, M., Niketić, M.,& Petrović, S.. (2018). DPPH radical scavenging potential of the root essential oils of five Heracleum L. taxa. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts Institute of Botany and Botanical Garden Jevremovac, Belgrade., 42(1 supplement), 131-131. https://hdl.handle.net/21.15107/rcub_farfar_5243
Ušjak L, Drobac M, Niketić M, Petrović S. DPPH radical scavenging potential of the root essential oils of five Heracleum L. taxa. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts. 2018;42(1 supplement):131-131. https://hdl.handle.net/21.15107/rcub_farfar_5243 .
Ušjak, Ljuboš, Drobac, Milica, Niketić, Marjan, Petrović, Silvana, "DPPH radical scavenging potential of the root essential oils of five Heracleum L. taxa" in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 42, no. 1 supplement (2018):131-131, https://hdl.handle.net/21.15107/rcub_farfar_5243 .