Is erucin a promising bioactive against renal cell carcinoma?
Апстракт
A large number of epidemiological studies have linked cruciferous vegetable consumption to
a reduced risk of various types of cancer, including renal cell carcinoma. Organosulfur
compounds from cruciferous vegetables, glucosinolates and their metabolic breakdown
derivatives, isothiocyanates and indole-3-carbinol, rise scientific interest by exerting unique
anticancer properties. Erucin (ER) is an isothiocyanate that is generated by enzymatic
hydrolyzes of glucoerucin, a glucosinolate predominant found in rocket species, or by in vivo
reduction of sulforaphane, its structural oxidized analog. In the present study, the inhibitory
effects of ER on renal cancer cell viability, migration and invasion were investigated. The 786-
O human renal cancer cell line and the Vero normal-like cells were treated with different
concentrations of ER (10-100 μM). Cell viability was determined using the MTT and PI assays.
The intracellular level of reactive oxygen species was evaluated using
di...chlorodihydrofluorescein diacetate. The collective cell migration and
chemotaxis/chemoinvasion were studied by a wound healing and transwell assay, respectively.
ER induced a concentration-dependent decrease in cell viability, with more cytotoxicity for
786-O cells than against Vero cells. Non-cytotoxic concentration of ER significantly reduced
cell migration rate, chemotaxis and invasiveness potential of 786-O cells. The observed
favorable anticancer potential of ER against human renal carcinoma in vitro requires further
investigation.
Извор:
Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade, 2020, 30-30Издавач:
- University of Belgrade, Belgrade, Serbia
Финансирање / пројекти:
- STSM Grant from COST Action NutRedOx, CA16112
- Fundação para a Ciência e a Tecnologia (FCT, Portugal), through funding UID/DTP/04567/2016 to CBIOS
Напомена:
- Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade, March 2-3, 2020
Институција/група
PharmacyTY - CONF AU - Vidović, Bojana AU - Guerreiro, I. AU - Costa, J.G. AU - Oliveira, N.G. AU - Saraiva, N. AU - Fernandes, A.S. PY - 2020 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5396 AB - A large number of epidemiological studies have linked cruciferous vegetable consumption to a reduced risk of various types of cancer, including renal cell carcinoma. Organosulfur compounds from cruciferous vegetables, glucosinolates and their metabolic breakdown derivatives, isothiocyanates and indole-3-carbinol, rise scientific interest by exerting unique anticancer properties. Erucin (ER) is an isothiocyanate that is generated by enzymatic hydrolyzes of glucoerucin, a glucosinolate predominant found in rocket species, or by in vivo reduction of sulforaphane, its structural oxidized analog. In the present study, the inhibitory effects of ER on renal cancer cell viability, migration and invasion were investigated. The 786- O human renal cancer cell line and the Vero normal-like cells were treated with different concentrations of ER (10-100 μM). Cell viability was determined using the MTT and PI assays. The intracellular level of reactive oxygen species was evaluated using dichlorodihydrofluorescein diacetate. The collective cell migration and chemotaxis/chemoinvasion were studied by a wound healing and transwell assay, respectively. ER induced a concentration-dependent decrease in cell viability, with more cytotoxicity for 786-O cells than against Vero cells. Non-cytotoxic concentration of ER significantly reduced cell migration rate, chemotaxis and invasiveness potential of 786-O cells. The observed favorable anticancer potential of ER against human renal carcinoma in vitro requires further investigation. PB - University of Belgrade, Belgrade, Serbia C3 - Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade T1 - Is erucin a promising bioactive against renal cell carcinoma? SP - 30 EP - 30 UR - https://hdl.handle.net/21.15107/rcub_farfar_5396 ER -
@conference{ author = "Vidović, Bojana and Guerreiro, I. and Costa, J.G. and Oliveira, N.G. and Saraiva, N. and Fernandes, A.S.", year = "2020", abstract = "A large number of epidemiological studies have linked cruciferous vegetable consumption to a reduced risk of various types of cancer, including renal cell carcinoma. Organosulfur compounds from cruciferous vegetables, glucosinolates and their metabolic breakdown derivatives, isothiocyanates and indole-3-carbinol, rise scientific interest by exerting unique anticancer properties. Erucin (ER) is an isothiocyanate that is generated by enzymatic hydrolyzes of glucoerucin, a glucosinolate predominant found in rocket species, or by in vivo reduction of sulforaphane, its structural oxidized analog. In the present study, the inhibitory effects of ER on renal cancer cell viability, migration and invasion were investigated. The 786- O human renal cancer cell line and the Vero normal-like cells were treated with different concentrations of ER (10-100 μM). Cell viability was determined using the MTT and PI assays. The intracellular level of reactive oxygen species was evaluated using dichlorodihydrofluorescein diacetate. The collective cell migration and chemotaxis/chemoinvasion were studied by a wound healing and transwell assay, respectively. ER induced a concentration-dependent decrease in cell viability, with more cytotoxicity for 786-O cells than against Vero cells. Non-cytotoxic concentration of ER significantly reduced cell migration rate, chemotaxis and invasiveness potential of 786-O cells. The observed favorable anticancer potential of ER against human renal carcinoma in vitro requires further investigation.", publisher = "University of Belgrade, Belgrade, Serbia", journal = "Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade", title = "Is erucin a promising bioactive against renal cell carcinoma?", pages = "30-30", url = "https://hdl.handle.net/21.15107/rcub_farfar_5396" }
Vidović, B., Guerreiro, I., Costa, J.G., Oliveira, N.G., Saraiva, N.,& Fernandes, A.S.. (2020). Is erucin a promising bioactive against renal cell carcinoma?. in Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade University of Belgrade, Belgrade, Serbia., 30-30. https://hdl.handle.net/21.15107/rcub_farfar_5396
Vidović B, Guerreiro I, Costa J, Oliveira N, Saraiva N, Fernandes A. Is erucin a promising bioactive against renal cell carcinoma?. in Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade. 2020;:30-30. https://hdl.handle.net/21.15107/rcub_farfar_5396 .
Vidović, Bojana, Guerreiro, I., Costa, J.G., Oliveira, N.G., Saraiva, N., Fernandes, A.S., "Is erucin a promising bioactive against renal cell carcinoma?" in Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade (2020):30-30, https://hdl.handle.net/21.15107/rcub_farfar_5396 .