High-performance thin-layer chromatography determination of some antimycotic imidazole derivatives and preservatives in medicinal creams and a gel
Abstract
Simple and reliable thin-layer chromatography-densitometry methods for determination of antimycotics (bifonazole, clotrimazole, and miconazole) and preservatives (benzyl alcohol and benzoic acid) were developed. The pairs bifonazole/benzyl alcohol, clotrimazole/benzyl alcohol, and miconazole/benzoic acid were determined simultaneously. The following mobile phases were used: ethyl acetate-n-heptane-methanol-diethylamine (3 + 4.5 + 1 + 0.2, v/v/v/v) for bifonazole and benzyl alcohol; n-butyl acetate-n-heptane-methanol-dietylamine (3 + 4.5 + 1 + 0.2, v/v/v/v) for clotrimazole and benzyl alcohol; and n-butyl acetate-carbon tetrachloride-methanol-diethylamine (3 + 6 + 2.5 + 0.5, v/v/v/v) for miconazole and benzoic acid. The chromatographic zones on silica gel plates were scanned in the reflectance/absorbance mode at 230 nm (bifonazole, benzyl alcohol, miconazole, and benzoic acid) and 210 nm (clotrimazole and benzyl alcohol). The recovery for all substances ranged from 98.7 to 100.7%. The l...imits of detection and quantitation were 0.03 to 0.2 mu g and 0.1 to 0.5 mu g/spot, respectively. The proposed methods were applied for determination of antimycotics and preservatives in commercially available pharmaceuticals.
Source:
Journal of AOAC International, 2005, 88, 5, 1544-1548Publisher:
- AOAC Int, Gaithersburg
Funding / projects:
- Ministry for Science and Environmental Protection of Serbia, Grant #1458.
DOI: 10.1093/jaoac/88.5.1544
ISSN: 1060-3271
PubMed: 16386007
WoS: 000232578700036
Scopus: 2-s2.0-27644500100
Collections
Institution/Community
PharmacyTY - JOUR AU - Čakar, Mira AU - Popović, Gordana AU - Agbaba, Danica PY - 2005 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/612 AB - Simple and reliable thin-layer chromatography-densitometry methods for determination of antimycotics (bifonazole, clotrimazole, and miconazole) and preservatives (benzyl alcohol and benzoic acid) were developed. The pairs bifonazole/benzyl alcohol, clotrimazole/benzyl alcohol, and miconazole/benzoic acid were determined simultaneously. The following mobile phases were used: ethyl acetate-n-heptane-methanol-diethylamine (3 + 4.5 + 1 + 0.2, v/v/v/v) for bifonazole and benzyl alcohol; n-butyl acetate-n-heptane-methanol-dietylamine (3 + 4.5 + 1 + 0.2, v/v/v/v) for clotrimazole and benzyl alcohol; and n-butyl acetate-carbon tetrachloride-methanol-diethylamine (3 + 6 + 2.5 + 0.5, v/v/v/v) for miconazole and benzoic acid. The chromatographic zones on silica gel plates were scanned in the reflectance/absorbance mode at 230 nm (bifonazole, benzyl alcohol, miconazole, and benzoic acid) and 210 nm (clotrimazole and benzyl alcohol). The recovery for all substances ranged from 98.7 to 100.7%. The limits of detection and quantitation were 0.03 to 0.2 mu g and 0.1 to 0.5 mu g/spot, respectively. The proposed methods were applied for determination of antimycotics and preservatives in commercially available pharmaceuticals. PB - AOAC Int, Gaithersburg T2 - Journal of AOAC International T1 - High-performance thin-layer chromatography determination of some antimycotic imidazole derivatives and preservatives in medicinal creams and a gel VL - 88 IS - 5 SP - 1544 EP - 1548 DO - 10.1093/jaoac/88.5.1544 ER -
@article{ author = "Čakar, Mira and Popović, Gordana and Agbaba, Danica", year = "2005", abstract = "Simple and reliable thin-layer chromatography-densitometry methods for determination of antimycotics (bifonazole, clotrimazole, and miconazole) and preservatives (benzyl alcohol and benzoic acid) were developed. The pairs bifonazole/benzyl alcohol, clotrimazole/benzyl alcohol, and miconazole/benzoic acid were determined simultaneously. The following mobile phases were used: ethyl acetate-n-heptane-methanol-diethylamine (3 + 4.5 + 1 + 0.2, v/v/v/v) for bifonazole and benzyl alcohol; n-butyl acetate-n-heptane-methanol-dietylamine (3 + 4.5 + 1 + 0.2, v/v/v/v) for clotrimazole and benzyl alcohol; and n-butyl acetate-carbon tetrachloride-methanol-diethylamine (3 + 6 + 2.5 + 0.5, v/v/v/v) for miconazole and benzoic acid. The chromatographic zones on silica gel plates were scanned in the reflectance/absorbance mode at 230 nm (bifonazole, benzyl alcohol, miconazole, and benzoic acid) and 210 nm (clotrimazole and benzyl alcohol). The recovery for all substances ranged from 98.7 to 100.7%. The limits of detection and quantitation were 0.03 to 0.2 mu g and 0.1 to 0.5 mu g/spot, respectively. The proposed methods were applied for determination of antimycotics and preservatives in commercially available pharmaceuticals.", publisher = "AOAC Int, Gaithersburg", journal = "Journal of AOAC International", title = "High-performance thin-layer chromatography determination of some antimycotic imidazole derivatives and preservatives in medicinal creams and a gel", volume = "88", number = "5", pages = "1544-1548", doi = "10.1093/jaoac/88.5.1544" }
Čakar, M., Popović, G.,& Agbaba, D.. (2005). High-performance thin-layer chromatography determination of some antimycotic imidazole derivatives and preservatives in medicinal creams and a gel. in Journal of AOAC International AOAC Int, Gaithersburg., 88(5), 1544-1548. https://doi.org/10.1093/jaoac/88.5.1544
Čakar M, Popović G, Agbaba D. High-performance thin-layer chromatography determination of some antimycotic imidazole derivatives and preservatives in medicinal creams and a gel. in Journal of AOAC International. 2005;88(5):1544-1548. doi:10.1093/jaoac/88.5.1544 .
Čakar, Mira, Popović, Gordana, Agbaba, Danica, "High-performance thin-layer chromatography determination of some antimycotic imidazole derivatives and preservatives in medicinal creams and a gel" in Journal of AOAC International, 88, no. 5 (2005):1544-1548, https://doi.org/10.1093/jaoac/88.5.1544 . .