Influence of thermal treatment on polyphenolic compounds and antioxidant properties of Quercus robur kernels
Uticaj termičkog tretmana na polifenolne sastojke i antioksidantna svojstva semena lužnjaka, Quercus robur
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Polyphenolic compounds and antioxidant properties of dried MeOH extracts of
native (QN) and thermally treated (QT) kernels of English Oak, Quercus robur were
investigated. The content of total and simple polyphenols and tannins was determined
spectrophotometrically using FC-reagentr, while gallic acid content was estimatecl usipg
HPLC2. After thermal treatment tannin content was decreased (from 20,4 to 19,70 ),
with significant increase of simple polyphenols and gallic acid content (from 1,9 to
4,004, and from 0,5 to 5,lYo, respectively).
Antioxidant activity was estimated using FRAP test (total antioxidant activity,
TAA), scavenging of DPPH radical and TBA test (inhibition of lipid peroxidation, LP
in liposomes)r.
Sample-s QN and QT showed substantial antioxidant activify. TAA was 2.44 and.
3.76 pmol F.'*/mg, respectively. Comparing to quercetin and L-ascorbic acid these
values were ca 2-3 times lower. Both samples concentration-dependently scavenged
stable DPPH radical (...IC5s:8.04 and 5.77 pglml), and inhibited LP (IC50:32.13 and
I 6.39 1tg/m| respectively).
Higher antioxidant activity of extract of thermally treated kernels (QT) could be
explained as a consequence of thermal degradation of hydrolysable tannins and,
therefore, increased gallic acid content.
Ispitivani su polifenolni sastojci i antioksidantna svojstva suvih MeOI.l
ekstrakata nativnog (QN) i termidki tretiranog (QT) semena luZnjaka, euercus robur.
SadrZaj ukupnih i netaninskih polifenola i tanina odredivan je spektiofotometrijski,
kori5cenjem Folin-Ciocalteu reagensa', a sadraaj galne kiseline tecnonr
hromatografijom (HPLC)2. Konstatovano je da nakon termicktg tretmana sadrZaj tanina
opada (sa 20,4 na 19,7o ), a znalajno raste kolidina netaninskih polifenola 1sa f ,9 na
4,0oA) i galne kiseline (sa 0,5 na 5 ,loh).
Za procenu antioksidante aktivnosti odredivani su: ukupna antioksidanta
aktivnost, UAA (FRAP test), sposobnost neutralizacije DPPH radikala i inhibitorni
efekat na lipidnu peroksidaciju (LP) u lipozomima (TBA tesr).r
Uzorci QN i QT ispoljili su znadajnu antioksidantnu aktivnost, i to UAA je
iznosila 2,44 i 3,76 pmol F.'*/rg, respektivno. Dobijene vrednosti aktivnosti su oko 2-
3 puta niZe u poredenju sa kvercetinom i vitaminom C. lJzorci eN i eT su
kon...centraciono zavisno neutralisali DPPH radikal (IC5s:8,04 i 5,77 pglml) i inhibirali
LP (ICs0:32,13 i I 6,39 ltglml, respektivno).
Veia antioksidantna aktivnost ekstrakta termidki tretiranog semena (eT), moLe
se objasniti veiim sadrZajem galne kiseline, koja se oslobada razgradnjom
hidrolizujucih tanina tokom termidke obrade.
Source:
Arhiv za farmaciju, 2006, 56, 5, 932-933Publisher:
- Savez farmaceutskih udruženja Srbije, Beograd
Note:
- IV Kongres farmaceuta Srbije sa međunarodnim učešćem, 29. novembar – 2. decembar 2006 godine, Beograd
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Institution/Community
PharmacyTY - CONF AU - Rakić, S. AU - Petrović, Silvana AU - Kukić, Jelena AU - Jadranin, Milka AU - Tešević, Vele PY - 2006 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/789 AB - Polyphenolic compounds and antioxidant properties of dried MeOH extracts of native (QN) and thermally treated (QT) kernels of English Oak, Quercus robur were investigated. The content of total and simple polyphenols and tannins was determined spectrophotometrically using FC-reagentr, while gallic acid content was estimatecl usipg HPLC2. After thermal treatment tannin content was decreased (from 20,4 to 19,70 ), with significant increase of simple polyphenols and gallic acid content (from 1,9 to 4,004, and from 0,5 to 5,lYo, respectively). Antioxidant activity was estimated using FRAP test (total antioxidant activity, TAA), scavenging of DPPH radical and TBA test (inhibition of lipid peroxidation, LP in liposomes)r. Sample-s QN and QT showed substantial antioxidant activify. TAA was 2.44 and. 3.76 pmol F.'*/mg, respectively. Comparing to quercetin and L-ascorbic acid these values were ca 2-3 times lower. Both samples concentration-dependently scavenged stable DPPH radical (IC5s:8.04 and 5.77 pglml), and inhibited LP (IC50:32.13 and I 6.39 1tg/m| respectively). Higher antioxidant activity of extract of thermally treated kernels (QT) could be explained as a consequence of thermal degradation of hydrolysable tannins and, therefore, increased gallic acid content. AB - Ispitivani su polifenolni sastojci i antioksidantna svojstva suvih MeOI.l ekstrakata nativnog (QN) i termidki tretiranog (QT) semena luZnjaka, euercus robur. SadrZaj ukupnih i netaninskih polifenola i tanina odredivan je spektiofotometrijski, kori5cenjem Folin-Ciocalteu reagensa', a sadraaj galne kiseline tecnonr hromatografijom (HPLC)2. Konstatovano je da nakon termicktg tretmana sadrZaj tanina opada (sa 20,4 na 19,7o ), a znalajno raste kolidina netaninskih polifenola 1sa f ,9 na 4,0oA) i galne kiseline (sa 0,5 na 5 ,loh). Za procenu antioksidante aktivnosti odredivani su: ukupna antioksidanta aktivnost, UAA (FRAP test), sposobnost neutralizacije DPPH radikala i inhibitorni efekat na lipidnu peroksidaciju (LP) u lipozomima (TBA tesr).r Uzorci QN i QT ispoljili su znadajnu antioksidantnu aktivnost, i to UAA je iznosila 2,44 i 3,76 pmol F.'*/rg, respektivno. Dobijene vrednosti aktivnosti su oko 2- 3 puta niZe u poredenju sa kvercetinom i vitaminom C. lJzorci eN i eT su koncentraciono zavisno neutralisali DPPH radikal (IC5s:8,04 i 5,77 pglml) i inhibirali LP (ICs0:32,13 i I 6,39 ltglml, respektivno). Veia antioksidantna aktivnost ekstrakta termidki tretiranog semena (eT), moLe se objasniti veiim sadrZajem galne kiseline, koja se oslobada razgradnjom hidrolizujucih tanina tokom termidke obrade. PB - Savez farmaceutskih udruženja Srbije, Beograd C3 - Arhiv za farmaciju T1 - Influence of thermal treatment on polyphenolic compounds and antioxidant properties of Quercus robur kernels T1 - Uticaj termičkog tretmana na polifenolne sastojke i antioksidantna svojstva semena lužnjaka, Quercus robur VL - 56 IS - 5 SP - 932 EP - 933 UR - https://hdl.handle.net/21.15107/rcub_farfar_789 ER -
@conference{ author = "Rakić, S. and Petrović, Silvana and Kukić, Jelena and Jadranin, Milka and Tešević, Vele", year = "2006", abstract = "Polyphenolic compounds and antioxidant properties of dried MeOH extracts of native (QN) and thermally treated (QT) kernels of English Oak, Quercus robur were investigated. The content of total and simple polyphenols and tannins was determined spectrophotometrically using FC-reagentr, while gallic acid content was estimatecl usipg HPLC2. After thermal treatment tannin content was decreased (from 20,4 to 19,70 ), with significant increase of simple polyphenols and gallic acid content (from 1,9 to 4,004, and from 0,5 to 5,lYo, respectively). Antioxidant activity was estimated using FRAP test (total antioxidant activity, TAA), scavenging of DPPH radical and TBA test (inhibition of lipid peroxidation, LP in liposomes)r. Sample-s QN and QT showed substantial antioxidant activify. TAA was 2.44 and. 3.76 pmol F.'*/mg, respectively. Comparing to quercetin and L-ascorbic acid these values were ca 2-3 times lower. Both samples concentration-dependently scavenged stable DPPH radical (IC5s:8.04 and 5.77 pglml), and inhibited LP (IC50:32.13 and I 6.39 1tg/m| respectively). Higher antioxidant activity of extract of thermally treated kernels (QT) could be explained as a consequence of thermal degradation of hydrolysable tannins and, therefore, increased gallic acid content., Ispitivani su polifenolni sastojci i antioksidantna svojstva suvih MeOI.l ekstrakata nativnog (QN) i termidki tretiranog (QT) semena luZnjaka, euercus robur. SadrZaj ukupnih i netaninskih polifenola i tanina odredivan je spektiofotometrijski, kori5cenjem Folin-Ciocalteu reagensa', a sadraaj galne kiseline tecnonr hromatografijom (HPLC)2. Konstatovano je da nakon termicktg tretmana sadrZaj tanina opada (sa 20,4 na 19,7o ), a znalajno raste kolidina netaninskih polifenola 1sa f ,9 na 4,0oA) i galne kiseline (sa 0,5 na 5 ,loh). Za procenu antioksidante aktivnosti odredivani su: ukupna antioksidanta aktivnost, UAA (FRAP test), sposobnost neutralizacije DPPH radikala i inhibitorni efekat na lipidnu peroksidaciju (LP) u lipozomima (TBA tesr).r Uzorci QN i QT ispoljili su znadajnu antioksidantnu aktivnost, i to UAA je iznosila 2,44 i 3,76 pmol F.'*/rg, respektivno. Dobijene vrednosti aktivnosti su oko 2- 3 puta niZe u poredenju sa kvercetinom i vitaminom C. lJzorci eN i eT su koncentraciono zavisno neutralisali DPPH radikal (IC5s:8,04 i 5,77 pglml) i inhibirali LP (ICs0:32,13 i I 6,39 ltglml, respektivno). Veia antioksidantna aktivnost ekstrakta termidki tretiranog semena (eT), moLe se objasniti veiim sadrZajem galne kiseline, koja se oslobada razgradnjom hidrolizujucih tanina tokom termidke obrade.", publisher = "Savez farmaceutskih udruženja Srbije, Beograd", journal = "Arhiv za farmaciju", title = "Influence of thermal treatment on polyphenolic compounds and antioxidant properties of Quercus robur kernels, Uticaj termičkog tretmana na polifenolne sastojke i antioksidantna svojstva semena lužnjaka, Quercus robur", volume = "56", number = "5", pages = "932-933", url = "https://hdl.handle.net/21.15107/rcub_farfar_789" }
Rakić, S., Petrović, S., Kukić, J., Jadranin, M.,& Tešević, V.. (2006). Influence of thermal treatment on polyphenolic compounds and antioxidant properties of Quercus robur kernels. in Arhiv za farmaciju Savez farmaceutskih udruženja Srbije, Beograd., 56(5), 932-933. https://hdl.handle.net/21.15107/rcub_farfar_789
Rakić S, Petrović S, Kukić J, Jadranin M, Tešević V. Influence of thermal treatment on polyphenolic compounds and antioxidant properties of Quercus robur kernels. in Arhiv za farmaciju. 2006;56(5):932-933. https://hdl.handle.net/21.15107/rcub_farfar_789 .
Rakić, S., Petrović, Silvana, Kukić, Jelena, Jadranin, Milka, Tešević, Vele, "Influence of thermal treatment on polyphenolic compounds and antioxidant properties of Quercus robur kernels" in Arhiv za farmaciju, 56, no. 5 (2006):932-933, https://hdl.handle.net/21.15107/rcub_farfar_789 .