Comparison of liquid chromatography with fluorescence detection to liquid chromatography-mass spectrometry for the determination of fluoxetine and norfluoxetine in human plasma
Abstract
A comparison study on fluoxetine (FL) and norfluoxetine (NORFL) quantitation in human plasma was carried out between the recently developed liquid chromatographic method with fluorescence detection (LC-FLD) and an earlier established liquid chromatography-mass spectrometry (LC-MS) laboratory procedure. Comparative method evaluation was based on the analysis of plasma samples obtained from Parkinsonian patients receiving 20 mg of FL per day. The LC-FLD method involves a two-step liquid extraction procedure without any derivatization, followed by direct chromatography on a Zorbax C8 reversed-phase column. The analytical results are discussed in terms of the method validation and the corresponding experimental protocol (r >= 0.998; CV lt 9%; LOQ 20 mu g/l). There was good correlation between FL, as well as NORFL, plasma levels as determined by the LC-MS and LC-FLD techniques (r = 0.9597, N = 16 and r = 0.9852, N = 14 for FL and NORFL, respectively). The results confirm that direct FL/NO...RFL fluorimetric determination is acceptable for routine use in pharmacokinetic and clinical studies.
Keywords:
fluoxetine / norfluoxetine / liquid chromatography / fluorescence detection / mass spectrometry / human plasma / pharmacokinetic analysisSource:
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2006, 830, 2, 372-376Publisher:
- Elsevier Science BV, Amsterdam
DOI: 10.1016/j.jchromb.2005.11.034
ISSN: 1570-0232
PubMed: 16356789
WoS: 000234864300029
Scopus: 2-s2.0-30444455896
Collections
Institution/Community
PharmacyTY - JOUR AU - Kovacević, I AU - Miljković, Branislava AU - Jovanović, D AU - Prostran, Milica PY - 2006 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/845 AB - A comparison study on fluoxetine (FL) and norfluoxetine (NORFL) quantitation in human plasma was carried out between the recently developed liquid chromatographic method with fluorescence detection (LC-FLD) and an earlier established liquid chromatography-mass spectrometry (LC-MS) laboratory procedure. Comparative method evaluation was based on the analysis of plasma samples obtained from Parkinsonian patients receiving 20 mg of FL per day. The LC-FLD method involves a two-step liquid extraction procedure without any derivatization, followed by direct chromatography on a Zorbax C8 reversed-phase column. The analytical results are discussed in terms of the method validation and the corresponding experimental protocol (r >= 0.998; CV lt 9%; LOQ 20 mu g/l). There was good correlation between FL, as well as NORFL, plasma levels as determined by the LC-MS and LC-FLD techniques (r = 0.9597, N = 16 and r = 0.9852, N = 14 for FL and NORFL, respectively). The results confirm that direct FL/NORFL fluorimetric determination is acceptable for routine use in pharmacokinetic and clinical studies. PB - Elsevier Science BV, Amsterdam T2 - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences T1 - Comparison of liquid chromatography with fluorescence detection to liquid chromatography-mass spectrometry for the determination of fluoxetine and norfluoxetine in human plasma VL - 830 IS - 2 SP - 372 EP - 376 DO - 10.1016/j.jchromb.2005.11.034 ER -
@article{ author = "Kovacević, I and Miljković, Branislava and Jovanović, D and Prostran, Milica", year = "2006", abstract = "A comparison study on fluoxetine (FL) and norfluoxetine (NORFL) quantitation in human plasma was carried out between the recently developed liquid chromatographic method with fluorescence detection (LC-FLD) and an earlier established liquid chromatography-mass spectrometry (LC-MS) laboratory procedure. Comparative method evaluation was based on the analysis of plasma samples obtained from Parkinsonian patients receiving 20 mg of FL per day. The LC-FLD method involves a two-step liquid extraction procedure without any derivatization, followed by direct chromatography on a Zorbax C8 reversed-phase column. The analytical results are discussed in terms of the method validation and the corresponding experimental protocol (r >= 0.998; CV lt 9%; LOQ 20 mu g/l). There was good correlation between FL, as well as NORFL, plasma levels as determined by the LC-MS and LC-FLD techniques (r = 0.9597, N = 16 and r = 0.9852, N = 14 for FL and NORFL, respectively). The results confirm that direct FL/NORFL fluorimetric determination is acceptable for routine use in pharmacokinetic and clinical studies.", publisher = "Elsevier Science BV, Amsterdam", journal = "Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences", title = "Comparison of liquid chromatography with fluorescence detection to liquid chromatography-mass spectrometry for the determination of fluoxetine and norfluoxetine in human plasma", volume = "830", number = "2", pages = "372-376", doi = "10.1016/j.jchromb.2005.11.034" }
Kovacević, I., Miljković, B., Jovanović, D.,& Prostran, M.. (2006). Comparison of liquid chromatography with fluorescence detection to liquid chromatography-mass spectrometry for the determination of fluoxetine and norfluoxetine in human plasma. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences Elsevier Science BV, Amsterdam., 830(2), 372-376. https://doi.org/10.1016/j.jchromb.2005.11.034
Kovacević I, Miljković B, Jovanović D, Prostran M. Comparison of liquid chromatography with fluorescence detection to liquid chromatography-mass spectrometry for the determination of fluoxetine and norfluoxetine in human plasma. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. 2006;830(2):372-376. doi:10.1016/j.jchromb.2005.11.034 .
Kovacević, I, Miljković, Branislava, Jovanović, D, Prostran, Milica, "Comparison of liquid chromatography with fluorescence detection to liquid chromatography-mass spectrometry for the determination of fluoxetine and norfluoxetine in human plasma" in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 830, no. 2 (2006):372-376, https://doi.org/10.1016/j.jchromb.2005.11.034 . .