An Experimental Design Approach to Selecting the Optimum HPLC Conditions for the Determination of 2-Arylimidazoline Derivatives
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2009
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Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In order to improve the chromatographic resolution (Rs) with a good analysis time, experimental designs were applied for multivariate optimization of the experimental conditions of an isocratic reversed phase high performance liquid chromatographic (RP-HPLC) method used for the simultaneous determination of seven 2-arylimidazoline derivatives, acting as vasoconstrictors and antihypertensives. Optimal conditions for the separation of the seven 2-arylimidazolines were obtained using a mixture of acetonitrile-triethylamine phosphate buffer (pH 4.0; 25mM) (30:70, v/v) as mobile phase at 25C. Here the 23 full factorial experimental design was employed to optimize RP-HPLC separation of the 2-arylimidazolines. The suggested RP-HPLC method is applicable for routine analysis of the 2-arylimidazoline derivatives under the same chromatographic conditions, which can shorten the time and the costs of the analysis as well. Detailed description of the method development and optimization given in this... paper will enhance the reproducibility of the established experimental conditions and simplify the process of some future methods developments.
Ključne reči:
Alpha agonist / Experimental design / Optimization of HPLC methodIzvor:
Journal of Liquid Chromatography & Related Technologies, 2009, 32, 5, 656-667Izdavač:
- Taylor & Francis Inc, Philadelphia
Finansiranje / projekti:
- Sinteza, kvantitativni odnosi između strukture/osobina i aktivnosti, fizičko-hemijska karakterizacija i analiza farmakološki aktivnih supstanci (RS-MESTD-MPN2006-2010-142071)
DOI: 10.1080/10826070802711113
ISSN: 1082-6076
WoS: 000262908400004
Scopus: 2-s2.0-61749097206
Institucija/grupa
PharmacyTY - JOUR AU - Vučićević, Katarina AU - Popović, Gordana AU - Nikolić, Katarina AU - Vovk, Irena AU - Agbaba, Danica PY - 2009 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1241 AB - In order to improve the chromatographic resolution (Rs) with a good analysis time, experimental designs were applied for multivariate optimization of the experimental conditions of an isocratic reversed phase high performance liquid chromatographic (RP-HPLC) method used for the simultaneous determination of seven 2-arylimidazoline derivatives, acting as vasoconstrictors and antihypertensives. Optimal conditions for the separation of the seven 2-arylimidazolines were obtained using a mixture of acetonitrile-triethylamine phosphate buffer (pH 4.0; 25mM) (30:70, v/v) as mobile phase at 25C. Here the 23 full factorial experimental design was employed to optimize RP-HPLC separation of the 2-arylimidazolines. The suggested RP-HPLC method is applicable for routine analysis of the 2-arylimidazoline derivatives under the same chromatographic conditions, which can shorten the time and the costs of the analysis as well. Detailed description of the method development and optimization given in this paper will enhance the reproducibility of the established experimental conditions and simplify the process of some future methods developments. PB - Taylor & Francis Inc, Philadelphia T2 - Journal of Liquid Chromatography & Related Technologies T1 - An Experimental Design Approach to Selecting the Optimum HPLC Conditions for the Determination of 2-Arylimidazoline Derivatives VL - 32 IS - 5 SP - 656 EP - 667 DO - 10.1080/10826070802711113 ER -
@article{ author = "Vučićević, Katarina and Popović, Gordana and Nikolić, Katarina and Vovk, Irena and Agbaba, Danica", year = "2009", abstract = "In order to improve the chromatographic resolution (Rs) with a good analysis time, experimental designs were applied for multivariate optimization of the experimental conditions of an isocratic reversed phase high performance liquid chromatographic (RP-HPLC) method used for the simultaneous determination of seven 2-arylimidazoline derivatives, acting as vasoconstrictors and antihypertensives. Optimal conditions for the separation of the seven 2-arylimidazolines were obtained using a mixture of acetonitrile-triethylamine phosphate buffer (pH 4.0; 25mM) (30:70, v/v) as mobile phase at 25C. Here the 23 full factorial experimental design was employed to optimize RP-HPLC separation of the 2-arylimidazolines. The suggested RP-HPLC method is applicable for routine analysis of the 2-arylimidazoline derivatives under the same chromatographic conditions, which can shorten the time and the costs of the analysis as well. Detailed description of the method development and optimization given in this paper will enhance the reproducibility of the established experimental conditions and simplify the process of some future methods developments.", publisher = "Taylor & Francis Inc, Philadelphia", journal = "Journal of Liquid Chromatography & Related Technologies", title = "An Experimental Design Approach to Selecting the Optimum HPLC Conditions for the Determination of 2-Arylimidazoline Derivatives", volume = "32", number = "5", pages = "656-667", doi = "10.1080/10826070802711113" }
Vučićević, K., Popović, G., Nikolić, K., Vovk, I.,& Agbaba, D.. (2009). An Experimental Design Approach to Selecting the Optimum HPLC Conditions for the Determination of 2-Arylimidazoline Derivatives. in Journal of Liquid Chromatography & Related Technologies Taylor & Francis Inc, Philadelphia., 32(5), 656-667. https://doi.org/10.1080/10826070802711113
Vučićević K, Popović G, Nikolić K, Vovk I, Agbaba D. An Experimental Design Approach to Selecting the Optimum HPLC Conditions for the Determination of 2-Arylimidazoline Derivatives. in Journal of Liquid Chromatography & Related Technologies. 2009;32(5):656-667. doi:10.1080/10826070802711113 .
Vučićević, Katarina, Popović, Gordana, Nikolić, Katarina, Vovk, Irena, Agbaba, Danica, "An Experimental Design Approach to Selecting the Optimum HPLC Conditions for the Determination of 2-Arylimidazoline Derivatives" in Journal of Liquid Chromatography & Related Technologies, 32, no. 5 (2009):656-667, https://doi.org/10.1080/10826070802711113 . .