Investigation of tropicamide and benzalkonium chloride stability using liquid chromatography
Samo za registrovane korisnike
2012
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In this study, a development of stability indicating method (SIM) that could be used to detect a decrease in the amount of tropicamide and benzalkonium chloride due to their degradation is described. In the method development, stress samples should be used. Benzalkanium chloride proved to be very stable under all stress conditions. Acid stress with 0.1 M HCl C led to 10% degradation of tropicamide alter >= 30 min. The alkaline stress with 1 M NaOH at 70 degrees C induced faster degradation and more than 20% of tropicamide degraded after 30 mm. On the other hand, tropicamide proved to be exceptionally stable to oxidative stress and no degradation was observed with 30% H2O2 even alter heating at 70 degrees C for 90 min. The mobile phase consisted of acetonitrile and water phase 70:30v/v (water phase: 5 mM sodium heptan sulphonate and 1% triethylamine MA) with phi of the mobile phase adjusted to 3.5 with ortophosphoric acid. The flaw rate was 2 mL min(-1). In order to confirm the method a...dequacy for intended purpose, the proposed method was validated. Finally, the proper validation of developed SIM performed according to the official guidelines proved that the method accomplishes its intended purpose.
Ključne reči:
benzalkonium chloride / eye drops / forced degradation studies / liquid chromatography / tropicamide / validationIzvor:
Journal of Liquid Chromatography & Related Technologies, 2012, 35, 1-4, 231-239Izdavač:
- Taylor & Francis Inc, Philadelphia
Finansiranje / projekti:
- Modelovanje različitih hromatografskih sistema sa hemometrijskim pristupom u farmaceutskoj analizi (RS-MESTD-Basic Research (BR or ON)-172052)
DOI: 10.1080/10826076.2011.597072
ISSN: 1082-6076
WoS: 000300967800018
Scopus: 2-s2.0-84856915415
Institucija/grupa
PharmacyTY - JOUR AU - Jovović, M. AU - Kostić, Nada AU - Jančić-Stojanović, Biljana AU - Malenović, Anđelija PY - 2012 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1696 AB - In this study, a development of stability indicating method (SIM) that could be used to detect a decrease in the amount of tropicamide and benzalkonium chloride due to their degradation is described. In the method development, stress samples should be used. Benzalkanium chloride proved to be very stable under all stress conditions. Acid stress with 0.1 M HCl C led to 10% degradation of tropicamide alter >= 30 min. The alkaline stress with 1 M NaOH at 70 degrees C induced faster degradation and more than 20% of tropicamide degraded after 30 mm. On the other hand, tropicamide proved to be exceptionally stable to oxidative stress and no degradation was observed with 30% H2O2 even alter heating at 70 degrees C for 90 min. The mobile phase consisted of acetonitrile and water phase 70:30v/v (water phase: 5 mM sodium heptan sulphonate and 1% triethylamine MA) with phi of the mobile phase adjusted to 3.5 with ortophosphoric acid. The flaw rate was 2 mL min(-1). In order to confirm the method adequacy for intended purpose, the proposed method was validated. Finally, the proper validation of developed SIM performed according to the official guidelines proved that the method accomplishes its intended purpose. PB - Taylor & Francis Inc, Philadelphia T2 - Journal of Liquid Chromatography & Related Technologies T1 - Investigation of tropicamide and benzalkonium chloride stability using liquid chromatography VL - 35 IS - 1-4 SP - 231 EP - 239 DO - 10.1080/10826076.2011.597072 ER -
@article{ author = "Jovović, M. and Kostić, Nada and Jančić-Stojanović, Biljana and Malenović, Anđelija", year = "2012", abstract = "In this study, a development of stability indicating method (SIM) that could be used to detect a decrease in the amount of tropicamide and benzalkonium chloride due to their degradation is described. In the method development, stress samples should be used. Benzalkanium chloride proved to be very stable under all stress conditions. Acid stress with 0.1 M HCl C led to 10% degradation of tropicamide alter >= 30 min. The alkaline stress with 1 M NaOH at 70 degrees C induced faster degradation and more than 20% of tropicamide degraded after 30 mm. On the other hand, tropicamide proved to be exceptionally stable to oxidative stress and no degradation was observed with 30% H2O2 even alter heating at 70 degrees C for 90 min. The mobile phase consisted of acetonitrile and water phase 70:30v/v (water phase: 5 mM sodium heptan sulphonate and 1% triethylamine MA) with phi of the mobile phase adjusted to 3.5 with ortophosphoric acid. The flaw rate was 2 mL min(-1). In order to confirm the method adequacy for intended purpose, the proposed method was validated. Finally, the proper validation of developed SIM performed according to the official guidelines proved that the method accomplishes its intended purpose.", publisher = "Taylor & Francis Inc, Philadelphia", journal = "Journal of Liquid Chromatography & Related Technologies", title = "Investigation of tropicamide and benzalkonium chloride stability using liquid chromatography", volume = "35", number = "1-4", pages = "231-239", doi = "10.1080/10826076.2011.597072" }
Jovović, M., Kostić, N., Jančić-Stojanović, B.,& Malenović, A.. (2012). Investigation of tropicamide and benzalkonium chloride stability using liquid chromatography. in Journal of Liquid Chromatography & Related Technologies Taylor & Francis Inc, Philadelphia., 35(1-4), 231-239. https://doi.org/10.1080/10826076.2011.597072
Jovović M, Kostić N, Jančić-Stojanović B, Malenović A. Investigation of tropicamide and benzalkonium chloride stability using liquid chromatography. in Journal of Liquid Chromatography & Related Technologies. 2012;35(1-4):231-239. doi:10.1080/10826076.2011.597072 .
Jovović, M., Kostić, Nada, Jančić-Stojanović, Biljana, Malenović, Anđelija, "Investigation of tropicamide and benzalkonium chloride stability using liquid chromatography" in Journal of Liquid Chromatography & Related Technologies, 35, no. 1-4 (2012):231-239, https://doi.org/10.1080/10826076.2011.597072 . .