Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms
2014
Autori
Pavun, LeposavaĐurđević, Predrag
Jelikić-Stankov, Milena
Đikanović, Daniela
Ćirić, Andrija
Uskoković-Marković, Snežana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
A simple, accurate and precise method based on the fluorescence properties of the aluminum(III)-quercetin complex, for the determination of quercetin, has been developed and validated. The complex has strong emission at pH 3.30, lambda(em) = 480 nm, with lambda(ex) = 420 nm. The linearity range of quercetin determination was 1.5-60.5 ng ml(-1) with LOD 0.09 ng ml(-1) and LOQ 0.27 ng ml(-1). Recovery values in the range of 99.9-100.2% indicate a good accuracy of the method. The established method was applied for the determination of quercetin in capsules, with a recovery value of 98.3%, standard deviation of 0.22% and coefficient of variation of 0.09%. The reliability of the method was checked by the newly developed RP-HPLC/UV method for capsules with the direct determination of quercetin after separation. The good agreement between the two methods indicates the applicability of the proposed spectrofluorimetric method for quercetin determination in pharmaceutical dosage forms, with high... reproducibility, and enables the direct and simple determination without its prior extraction from samples. The proposed spectrofluorimetric method has much better sensitivity and LOD and LOQ values that are about 1000 times lower than data reported in the literature.
Ključne reči:
quercetin / flavonoids / spectrofluorimetric determination / RP-HPLC / capsulesIzvor:
Macedonian Journal of Chemistry and Chemical Engineering, 2014, 33, 2, 209-215Izdavač:
- Soc Chemists Technologists Madeconia, Skopje
Finansiranje / projekti:
- Sinteza, modelovanje, fizičko-hemijske i biološke osobine organskih jedinjenja i odgovarajućih kompleksa metala (RS-MESTD-Basic Research (BR or ON)-172016)
DOI: 10.20450/mjcce.2014.496
ISSN: 1857-5552
WoS: 000356592500002
Scopus: 2-s2.0-84929326612
Institucija/grupa
PharmacyTY - JOUR AU - Pavun, Leposava AU - Đurđević, Predrag AU - Jelikić-Stankov, Milena AU - Đikanović, Daniela AU - Ćirić, Andrija AU - Uskoković-Marković, Snežana PY - 2014 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2116 AB - A simple, accurate and precise method based on the fluorescence properties of the aluminum(III)-quercetin complex, for the determination of quercetin, has been developed and validated. The complex has strong emission at pH 3.30, lambda(em) = 480 nm, with lambda(ex) = 420 nm. The linearity range of quercetin determination was 1.5-60.5 ng ml(-1) with LOD 0.09 ng ml(-1) and LOQ 0.27 ng ml(-1). Recovery values in the range of 99.9-100.2% indicate a good accuracy of the method. The established method was applied for the determination of quercetin in capsules, with a recovery value of 98.3%, standard deviation of 0.22% and coefficient of variation of 0.09%. The reliability of the method was checked by the newly developed RP-HPLC/UV method for capsules with the direct determination of quercetin after separation. The good agreement between the two methods indicates the applicability of the proposed spectrofluorimetric method for quercetin determination in pharmaceutical dosage forms, with high reproducibility, and enables the direct and simple determination without its prior extraction from samples. The proposed spectrofluorimetric method has much better sensitivity and LOD and LOQ values that are about 1000 times lower than data reported in the literature. PB - Soc Chemists Technologists Madeconia, Skopje T2 - Macedonian Journal of Chemistry and Chemical Engineering T1 - Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms VL - 33 IS - 2 SP - 209 EP - 215 DO - 10.20450/mjcce.2014.496 ER -
@article{ author = "Pavun, Leposava and Đurđević, Predrag and Jelikić-Stankov, Milena and Đikanović, Daniela and Ćirić, Andrija and Uskoković-Marković, Snežana", year = "2014", abstract = "A simple, accurate and precise method based on the fluorescence properties of the aluminum(III)-quercetin complex, for the determination of quercetin, has been developed and validated. The complex has strong emission at pH 3.30, lambda(em) = 480 nm, with lambda(ex) = 420 nm. The linearity range of quercetin determination was 1.5-60.5 ng ml(-1) with LOD 0.09 ng ml(-1) and LOQ 0.27 ng ml(-1). Recovery values in the range of 99.9-100.2% indicate a good accuracy of the method. The established method was applied for the determination of quercetin in capsules, with a recovery value of 98.3%, standard deviation of 0.22% and coefficient of variation of 0.09%. The reliability of the method was checked by the newly developed RP-HPLC/UV method for capsules with the direct determination of quercetin after separation. The good agreement between the two methods indicates the applicability of the proposed spectrofluorimetric method for quercetin determination in pharmaceutical dosage forms, with high reproducibility, and enables the direct and simple determination without its prior extraction from samples. The proposed spectrofluorimetric method has much better sensitivity and LOD and LOQ values that are about 1000 times lower than data reported in the literature.", publisher = "Soc Chemists Technologists Madeconia, Skopje", journal = "Macedonian Journal of Chemistry and Chemical Engineering", title = "Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms", volume = "33", number = "2", pages = "209-215", doi = "10.20450/mjcce.2014.496" }
Pavun, L., Đurđević, P., Jelikić-Stankov, M., Đikanović, D., Ćirić, A.,& Uskoković-Marković, S.. (2014). Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms. in Macedonian Journal of Chemistry and Chemical Engineering Soc Chemists Technologists Madeconia, Skopje., 33(2), 209-215. https://doi.org/10.20450/mjcce.2014.496
Pavun L, Đurđević P, Jelikić-Stankov M, Đikanović D, Ćirić A, Uskoković-Marković S. Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms. in Macedonian Journal of Chemistry and Chemical Engineering. 2014;33(2):209-215. doi:10.20450/mjcce.2014.496 .
Pavun, Leposava, Đurđević, Predrag, Jelikić-Stankov, Milena, Đikanović, Daniela, Ćirić, Andrija, Uskoković-Marković, Snežana, "Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms" in Macedonian Journal of Chemistry and Chemical Engineering, 33, no. 2 (2014):209-215, https://doi.org/10.20450/mjcce.2014.496 . .