Interactions between bioactive components determine antioxidant, cytotoxic and nutrigenomic activity of cocoa powder extract
Autori
Baranowska, MonikaSuliborska, Klaudia
Todorović, Vanja
Kusznierewicz, Barbara
Chrzanowski, Wojciech
Šobajić, Slađana
Bartoszek, Agnieszka
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Numerous studies have shown, rather disappointingly, that isolated bioactive phytochemicals are not as biologically effective as natural plant products. Such a discrepancy may be explained by the concept of food synergy, which was verified in this research for cocoa extract versus its major components with regard to cancer chemoprevention. The evaluation embraced the relationship between redox properties evaluated in cell-free systems with the aid of free radicals scavenging method and differential pulse voltammetry, and redox associated anticarcinogenic activities (cellular antioxidant activity, cytotoxicity, nutrigenomic activity) in human colon adenocarcinoma cell line exposed to either cocoa powder extract or artificial mixtures of cocoa bioactives at matching concentrations. In contrast to expectations, our results showed that the stepwise enrichment with antioxidants caused no gradual increase in the antioxidant activity of the model mixtures; also, these model mixtures did not r...each the reducing potential of cocoa in the cell-free systems or cellular model employed. Further, the biological activities examined in colon adenocarcinoma cells did not alter in a stepwise manner that could reflect the gradual changes in composition of bioactive ingredients. In conclusion, the experiments presented here showed that the growing complexity of a mixture of phytochemicals seems to create a new redox bioactive substance rather than enrich the mixture with new activities, characteristic of the compound added. It follows that no simple, predictable relationship can be expected between the chemopreventive potential and the composition of real food items containing a complicated set of non-toxic redox active ingredients. Our observations suggest that the interactions between different bioactive compounds and food matrix components are cooperating factors determining the final bioactivity of foods.
Ključne reči:
Antioxidants / Catechins / Chemoprevention / Cocoa powder / Food synergy / Redox biologyIzvor:
Free Radical Biology and Medicine, 2020, 154, 48-61Izdavač:
- Elsevier
Finansiranje / projekti:
- National Science Centre (Poland) through the MAESTRO 6 grant programme (application number 2014/ 14/A/ST4/00640)
- COST Action CA16112 supported by COST (European Cooperation in Science and Technology)
DOI: 10.1016/j.freeradbiomed.2020.04.022
ISSN: 0891-5849
WoS: 000537132500005
Scopus: 2-s2.0-85084365695
Institucija/grupa
PharmacyTY - JOUR AU - Baranowska, Monika AU - Suliborska, Klaudia AU - Todorović, Vanja AU - Kusznierewicz, Barbara AU - Chrzanowski, Wojciech AU - Šobajić, Slađana AU - Bartoszek, Agnieszka PY - 2020 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3584 AB - Numerous studies have shown, rather disappointingly, that isolated bioactive phytochemicals are not as biologically effective as natural plant products. Such a discrepancy may be explained by the concept of food synergy, which was verified in this research for cocoa extract versus its major components with regard to cancer chemoprevention. The evaluation embraced the relationship between redox properties evaluated in cell-free systems with the aid of free radicals scavenging method and differential pulse voltammetry, and redox associated anticarcinogenic activities (cellular antioxidant activity, cytotoxicity, nutrigenomic activity) in human colon adenocarcinoma cell line exposed to either cocoa powder extract or artificial mixtures of cocoa bioactives at matching concentrations. In contrast to expectations, our results showed that the stepwise enrichment with antioxidants caused no gradual increase in the antioxidant activity of the model mixtures; also, these model mixtures did not reach the reducing potential of cocoa in the cell-free systems or cellular model employed. Further, the biological activities examined in colon adenocarcinoma cells did not alter in a stepwise manner that could reflect the gradual changes in composition of bioactive ingredients. In conclusion, the experiments presented here showed that the growing complexity of a mixture of phytochemicals seems to create a new redox bioactive substance rather than enrich the mixture with new activities, characteristic of the compound added. It follows that no simple, predictable relationship can be expected between the chemopreventive potential and the composition of real food items containing a complicated set of non-toxic redox active ingredients. Our observations suggest that the interactions between different bioactive compounds and food matrix components are cooperating factors determining the final bioactivity of foods. PB - Elsevier T2 - Free Radical Biology and Medicine T1 - Interactions between bioactive components determine antioxidant, cytotoxic and nutrigenomic activity of cocoa powder extract VL - 154 SP - 48 EP - 61 DO - 10.1016/j.freeradbiomed.2020.04.022 ER -
@article{ author = "Baranowska, Monika and Suliborska, Klaudia and Todorović, Vanja and Kusznierewicz, Barbara and Chrzanowski, Wojciech and Šobajić, Slađana and Bartoszek, Agnieszka", year = "2020", abstract = "Numerous studies have shown, rather disappointingly, that isolated bioactive phytochemicals are not as biologically effective as natural plant products. Such a discrepancy may be explained by the concept of food synergy, which was verified in this research for cocoa extract versus its major components with regard to cancer chemoprevention. The evaluation embraced the relationship between redox properties evaluated in cell-free systems with the aid of free radicals scavenging method and differential pulse voltammetry, and redox associated anticarcinogenic activities (cellular antioxidant activity, cytotoxicity, nutrigenomic activity) in human colon adenocarcinoma cell line exposed to either cocoa powder extract or artificial mixtures of cocoa bioactives at matching concentrations. In contrast to expectations, our results showed that the stepwise enrichment with antioxidants caused no gradual increase in the antioxidant activity of the model mixtures; also, these model mixtures did not reach the reducing potential of cocoa in the cell-free systems or cellular model employed. Further, the biological activities examined in colon adenocarcinoma cells did not alter in a stepwise manner that could reflect the gradual changes in composition of bioactive ingredients. In conclusion, the experiments presented here showed that the growing complexity of a mixture of phytochemicals seems to create a new redox bioactive substance rather than enrich the mixture with new activities, characteristic of the compound added. It follows that no simple, predictable relationship can be expected between the chemopreventive potential and the composition of real food items containing a complicated set of non-toxic redox active ingredients. Our observations suggest that the interactions between different bioactive compounds and food matrix components are cooperating factors determining the final bioactivity of foods.", publisher = "Elsevier", journal = "Free Radical Biology and Medicine", title = "Interactions between bioactive components determine antioxidant, cytotoxic and nutrigenomic activity of cocoa powder extract", volume = "154", pages = "48-61", doi = "10.1016/j.freeradbiomed.2020.04.022" }
Baranowska, M., Suliborska, K., Todorović, V., Kusznierewicz, B., Chrzanowski, W., Šobajić, S.,& Bartoszek, A.. (2020). Interactions between bioactive components determine antioxidant, cytotoxic and nutrigenomic activity of cocoa powder extract. in Free Radical Biology and Medicine Elsevier., 154, 48-61. https://doi.org/10.1016/j.freeradbiomed.2020.04.022
Baranowska M, Suliborska K, Todorović V, Kusznierewicz B, Chrzanowski W, Šobajić S, Bartoszek A. Interactions between bioactive components determine antioxidant, cytotoxic and nutrigenomic activity of cocoa powder extract. in Free Radical Biology and Medicine. 2020;154:48-61. doi:10.1016/j.freeradbiomed.2020.04.022 .
Baranowska, Monika, Suliborska, Klaudia, Todorović, Vanja, Kusznierewicz, Barbara, Chrzanowski, Wojciech, Šobajić, Slađana, Bartoszek, Agnieszka, "Interactions between bioactive components determine antioxidant, cytotoxic and nutrigenomic activity of cocoa powder extract" in Free Radical Biology and Medicine, 154 (2020):48-61, https://doi.org/10.1016/j.freeradbiomed.2020.04.022 . .