Comparison of efficiency of eutectic mixture and conventional solvents for the extraction of hydroxycinnamic acid derivatives from the herb of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae)
Poređenje efikasnosti eutektičke smeše i konvencionalnih rastvarača u ekstrakciji derivata hidroksicimetne kiseline iz herbe Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae)
Authors
Arsenijević, JelenaDrobac, Milica
Slavkovska, Violeta
Dabetić, Nevena
Marcic, Christophe
Kovačević, Nada
Ražić, Slavica
Conference object (Published version)
Metadata
Show full item recordAbstract
Hydroxycinnamic acids (HCAs), such are dimeric rosmarinic acid (RA) and higher HCA
oligomers, are important active constituents of Lamiaceae plants. It was found that herb of
Satureja kitaibelii (Rtanj tea) contains substantial amounts of RA and HCA hexamer
clinopodic acid O (CAO) (1).
In this work, we compared extracting efficiencies of conventional solvents and a deep
eutectic solvent, composed of choline chloride and citric acid (ChCl:CitrA), for extraction of
RA and CAO from Rtanj tea. Commercial Rtanj tea sample was extracted (1:10) by sonication
(room temperature, 60 min) with aqueous ChCl:CitrA (35% and 65%), ethanol 50%, ethanol
96%, and water, as well as by previously optimized procedure (ethanol 46%, digestion 60 °C,
120 min) (1). Contents of RA and CAO were determined by HPLC using RA as external
standard. Extraction with ChCl:CitrA and 50% ethanol resulted in extracts rich in RA and
CAO; RA concentrations in these extracts were uniform (848-873 μg/mL), while extr...action of
CAO was slightly more effective with ChCl:CitrA (1122-1131 μg/mL) than with 50% ethanol
(925 μg/mL). These extracts contained significantly more of RA and CAO than the extract
obtained under optimized conditions (666 μg/mL RA, 725 μg/mL CAO). Differently, 96%
ethanol and water extracts contained substantially less RA (11-217 μg/mL) and CAO (4-10
μg/mL). Obtained results indicate potential of applied eutectic solvent in extraction of Rtanj
tea, for purpose of both quality control and preparation of extracts with potential application
in pharmaceutical, food or cosmetic industry, and are compliant with “green chemistry” and
“green pharmacy” principles.
Derivati hidroksicimetne kiseline (HCK), kao što su dimerna rozmarinska kiselina
(RK) i viši oligomeri HCK, su aktivni sastojci biljaka familije Lamiaceae. Ranije je
ustanovljeno da herba Satureja kitaibelii (rtanjski čaj) sadrži značajne količine RK i
heksamera HCK klinopodinske kiseline O (KKO) (1). U radu je upoređena ekstrakciona
efikasnost konvencionalnih rastvarača i duboke eutektičke smeše na bazi holin hlorida i
limunske kiseline (ChCl:CitrA) za ekstrakciju RK i KKO iz rtanjskog čaja. Komercijalni uzorak
rtanjskog čaja ekstrahovan je (1:10) postupkom sonikacije na sobnoj temperaturi tokom 60
min vodenim rastvorima ChCl:CitrA (35% i 65%), etanolom 50%, etanolom 96% i vodom,
kao i prethodno optimizovanim postupkom (etanol 46%, digestija na 60 °C, 120 min) (1).
Sadržaj RK i KKO određen je HPLC metodom, korišćenjem RK kao eksternog standarda.
Ekstrakcija primenom ChCl:CitrA i 50% etanola rezultirala je ekstraktima bogatim RK i KKO,
pri čemu su koncentracije RK u ovim eks...traktima bile ujednačene (848-873 μg/mL), dok je
za ekstrakciju KKO, ChCl:CitrA bila nešto efikasnija (1122-1131 μg/mL) od 50% etanola (925
μg/mL). Pored toga, ovi ekstrakti su sadržali veće količine RK i KKO od ekstrakta dobijenog
pod ranije optimizovanim uslovima (666 μg/mL RK, 725 μg/mL KKO). Sa druge strane,
ekstrakti dobijeni 96% etanolom i vodom sadržali su znatno manje količine RK (11-217
μg/mL) i KKO (4-10 μg/mL). Dobijeni rezultati ukazuju na potencijal primenjene eutektičke
smeše u ekstrakciji rtanjskog čaja, kako u cilju kontrole kvaliteta, tako i za pripremu
ekstrakata sa primenom u farmaceutskoj, prehrambenoj i kozmetičkoj industriji, što je u
skladu sa principima “zelene hemije” i “zelene farmacije”.
Source:
Arhiv za farmaciju, 2022, 72, 4 suplement, S481-S482Publisher:
- Savez farmaceutskih udruženja Srbije (SFUS)
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200161 (University of Belgrade, Faculty of Pharmacy) (RS-MESTD-inst-2020-200161)
Note:
- VIII Kongres farmaceuta Srbije sa međunarodnim učešćem, 12-15.10.2022. Beograd
Collections
Institution/Community
PharmacyTY - CONF AU - Arsenijević, Jelena AU - Drobac, Milica AU - Slavkovska, Violeta AU - Dabetić, Nevena AU - Marcic, Christophe AU - Kovačević, Nada AU - Ražić, Slavica PY - 2022 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4587 AB - Hydroxycinnamic acids (HCAs), such are dimeric rosmarinic acid (RA) and higher HCA oligomers, are important active constituents of Lamiaceae plants. It was found that herb of Satureja kitaibelii (Rtanj tea) contains substantial amounts of RA and HCA hexamer clinopodic acid O (CAO) (1). In this work, we compared extracting efficiencies of conventional solvents and a deep eutectic solvent, composed of choline chloride and citric acid (ChCl:CitrA), for extraction of RA and CAO from Rtanj tea. Commercial Rtanj tea sample was extracted (1:10) by sonication (room temperature, 60 min) with aqueous ChCl:CitrA (35% and 65%), ethanol 50%, ethanol 96%, and water, as well as by previously optimized procedure (ethanol 46%, digestion 60 °C, 120 min) (1). Contents of RA and CAO were determined by HPLC using RA as external standard. Extraction with ChCl:CitrA and 50% ethanol resulted in extracts rich in RA and CAO; RA concentrations in these extracts were uniform (848-873 μg/mL), while extraction of CAO was slightly more effective with ChCl:CitrA (1122-1131 μg/mL) than with 50% ethanol (925 μg/mL). These extracts contained significantly more of RA and CAO than the extract obtained under optimized conditions (666 μg/mL RA, 725 μg/mL CAO). Differently, 96% ethanol and water extracts contained substantially less RA (11-217 μg/mL) and CAO (4-10 μg/mL). Obtained results indicate potential of applied eutectic solvent in extraction of Rtanj tea, for purpose of both quality control and preparation of extracts with potential application in pharmaceutical, food or cosmetic industry, and are compliant with “green chemistry” and “green pharmacy” principles. AB - Derivati hidroksicimetne kiseline (HCK), kao što su dimerna rozmarinska kiselina (RK) i viši oligomeri HCK, su aktivni sastojci biljaka familije Lamiaceae. Ranije je ustanovljeno da herba Satureja kitaibelii (rtanjski čaj) sadrži značajne količine RK i heksamera HCK klinopodinske kiseline O (KKO) (1). U radu je upoređena ekstrakciona efikasnost konvencionalnih rastvarača i duboke eutektičke smeše na bazi holin hlorida i limunske kiseline (ChCl:CitrA) za ekstrakciju RK i KKO iz rtanjskog čaja. Komercijalni uzorak rtanjskog čaja ekstrahovan je (1:10) postupkom sonikacije na sobnoj temperaturi tokom 60 min vodenim rastvorima ChCl:CitrA (35% i 65%), etanolom 50%, etanolom 96% i vodom, kao i prethodno optimizovanim postupkom (etanol 46%, digestija na 60 °C, 120 min) (1). Sadržaj RK i KKO određen je HPLC metodom, korišćenjem RK kao eksternog standarda. Ekstrakcija primenom ChCl:CitrA i 50% etanola rezultirala je ekstraktima bogatim RK i KKO, pri čemu su koncentracije RK u ovim ekstraktima bile ujednačene (848-873 μg/mL), dok je za ekstrakciju KKO, ChCl:CitrA bila nešto efikasnija (1122-1131 μg/mL) od 50% etanola (925 μg/mL). Pored toga, ovi ekstrakti su sadržali veće količine RK i KKO od ekstrakta dobijenog pod ranije optimizovanim uslovima (666 μg/mL RK, 725 μg/mL KKO). Sa druge strane, ekstrakti dobijeni 96% etanolom i vodom sadržali su znatno manje količine RK (11-217 μg/mL) i KKO (4-10 μg/mL). Dobijeni rezultati ukazuju na potencijal primenjene eutektičke smeše u ekstrakciji rtanjskog čaja, kako u cilju kontrole kvaliteta, tako i za pripremu ekstrakata sa primenom u farmaceutskoj, prehrambenoj i kozmetičkoj industriji, što je u skladu sa principima “zelene hemije” i “zelene farmacije”. PB - Savez farmaceutskih udruženja Srbije (SFUS) C3 - Arhiv za farmaciju T1 - Comparison of efficiency of eutectic mixture and conventional solvents for the extraction of hydroxycinnamic acid derivatives from the herb of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae) T1 - Poređenje efikasnosti eutektičke smeše i konvencionalnih rastvarača u ekstrakciji derivata hidroksicimetne kiseline iz herbe Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae) VL - 72 IS - 4 suplement SP - S481 EP - S482 UR - https://hdl.handle.net/21.15107/rcub_farfar_4587 ER -
@conference{ author = "Arsenijević, Jelena and Drobac, Milica and Slavkovska, Violeta and Dabetić, Nevena and Marcic, Christophe and Kovačević, Nada and Ražić, Slavica", year = "2022", abstract = "Hydroxycinnamic acids (HCAs), such are dimeric rosmarinic acid (RA) and higher HCA oligomers, are important active constituents of Lamiaceae plants. It was found that herb of Satureja kitaibelii (Rtanj tea) contains substantial amounts of RA and HCA hexamer clinopodic acid O (CAO) (1). In this work, we compared extracting efficiencies of conventional solvents and a deep eutectic solvent, composed of choline chloride and citric acid (ChCl:CitrA), for extraction of RA and CAO from Rtanj tea. Commercial Rtanj tea sample was extracted (1:10) by sonication (room temperature, 60 min) with aqueous ChCl:CitrA (35% and 65%), ethanol 50%, ethanol 96%, and water, as well as by previously optimized procedure (ethanol 46%, digestion 60 °C, 120 min) (1). Contents of RA and CAO were determined by HPLC using RA as external standard. Extraction with ChCl:CitrA and 50% ethanol resulted in extracts rich in RA and CAO; RA concentrations in these extracts were uniform (848-873 μg/mL), while extraction of CAO was slightly more effective with ChCl:CitrA (1122-1131 μg/mL) than with 50% ethanol (925 μg/mL). These extracts contained significantly more of RA and CAO than the extract obtained under optimized conditions (666 μg/mL RA, 725 μg/mL CAO). Differently, 96% ethanol and water extracts contained substantially less RA (11-217 μg/mL) and CAO (4-10 μg/mL). Obtained results indicate potential of applied eutectic solvent in extraction of Rtanj tea, for purpose of both quality control and preparation of extracts with potential application in pharmaceutical, food or cosmetic industry, and are compliant with “green chemistry” and “green pharmacy” principles., Derivati hidroksicimetne kiseline (HCK), kao što su dimerna rozmarinska kiselina (RK) i viši oligomeri HCK, su aktivni sastojci biljaka familije Lamiaceae. Ranije je ustanovljeno da herba Satureja kitaibelii (rtanjski čaj) sadrži značajne količine RK i heksamera HCK klinopodinske kiseline O (KKO) (1). U radu je upoređena ekstrakciona efikasnost konvencionalnih rastvarača i duboke eutektičke smeše na bazi holin hlorida i limunske kiseline (ChCl:CitrA) za ekstrakciju RK i KKO iz rtanjskog čaja. Komercijalni uzorak rtanjskog čaja ekstrahovan je (1:10) postupkom sonikacije na sobnoj temperaturi tokom 60 min vodenim rastvorima ChCl:CitrA (35% i 65%), etanolom 50%, etanolom 96% i vodom, kao i prethodno optimizovanim postupkom (etanol 46%, digestija na 60 °C, 120 min) (1). Sadržaj RK i KKO određen je HPLC metodom, korišćenjem RK kao eksternog standarda. Ekstrakcija primenom ChCl:CitrA i 50% etanola rezultirala je ekstraktima bogatim RK i KKO, pri čemu su koncentracije RK u ovim ekstraktima bile ujednačene (848-873 μg/mL), dok je za ekstrakciju KKO, ChCl:CitrA bila nešto efikasnija (1122-1131 μg/mL) od 50% etanola (925 μg/mL). Pored toga, ovi ekstrakti su sadržali veće količine RK i KKO od ekstrakta dobijenog pod ranije optimizovanim uslovima (666 μg/mL RK, 725 μg/mL KKO). Sa druge strane, ekstrakti dobijeni 96% etanolom i vodom sadržali su znatno manje količine RK (11-217 μg/mL) i KKO (4-10 μg/mL). Dobijeni rezultati ukazuju na potencijal primenjene eutektičke smeše u ekstrakciji rtanjskog čaja, kako u cilju kontrole kvaliteta, tako i za pripremu ekstrakata sa primenom u farmaceutskoj, prehrambenoj i kozmetičkoj industriji, što je u skladu sa principima “zelene hemije” i “zelene farmacije”.", publisher = "Savez farmaceutskih udruženja Srbije (SFUS)", journal = "Arhiv za farmaciju", title = "Comparison of efficiency of eutectic mixture and conventional solvents for the extraction of hydroxycinnamic acid derivatives from the herb of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae), Poređenje efikasnosti eutektičke smeše i konvencionalnih rastvarača u ekstrakciji derivata hidroksicimetne kiseline iz herbe Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae)", volume = "72", number = "4 suplement", pages = "S481-S482", url = "https://hdl.handle.net/21.15107/rcub_farfar_4587" }
Arsenijević, J., Drobac, M., Slavkovska, V., Dabetić, N., Marcic, C., Kovačević, N.,& Ražić, S.. (2022). Comparison of efficiency of eutectic mixture and conventional solvents for the extraction of hydroxycinnamic acid derivatives from the herb of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae). in Arhiv za farmaciju Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S481-S482. https://hdl.handle.net/21.15107/rcub_farfar_4587
Arsenijević J, Drobac M, Slavkovska V, Dabetić N, Marcic C, Kovačević N, Ražić S. Comparison of efficiency of eutectic mixture and conventional solvents for the extraction of hydroxycinnamic acid derivatives from the herb of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae). in Arhiv za farmaciju. 2022;72(4 suplement):S481-S482. https://hdl.handle.net/21.15107/rcub_farfar_4587 .
Arsenijević, Jelena, Drobac, Milica, Slavkovska, Violeta, Dabetić, Nevena, Marcic, Christophe, Kovačević, Nada, Ražić, Slavica, "Comparison of efficiency of eutectic mixture and conventional solvents for the extraction of hydroxycinnamic acid derivatives from the herb of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae)" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S481-S482, https://hdl.handle.net/21.15107/rcub_farfar_4587 .