Differentiation of human dendritic cells from monocytes in vitro using granulocyte-macrophage colony stimulating factor and low concentration of interleukin-4
Diferencijacija humanih dendritičnih ćelija od monocita in vitro korišćenjem faktora stimulacije granulocitno-makrofagnih kolonija i niske koncentracije interleukina-4
2003
Аутори
Čolić, MiodragJandrić, Dušan
Stojić-Vukanić, Zorica
Antić-Stanković, Jelena
Popović, Petar
Vasilijić, Saša
Milosavljević, Petar
Balint, Bela
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Several laboratories have developed culture systems that allow the generation of large numbers of human dendritic cells (DC) from monocytes using granulocyte-macrophage colony stimulating factor (GM-CSF), and interleukin-4 (IL-4). In this work we provided evidence that GM-CSF (100 ng/ml) in combination with a low concentration of IL-4 (5 ng/ml) was efficient in the generation of immature, non-adherent, monocyte-derived DC as the same concentration of GM-CSF, and ten times higher concentration of IL-4 (50 ng/ml). This conclusion was based on the similar phenotype profile of DC such as the expression of CD1a, CD80, CD86, and HLA-DR, down-regulation of CD14, and the absence of CD83, as well as on their similar allostimulatory activity for T cells. A higher number of cells remained adherent in cultures with lower concentrations of IL-4 than in cultures with higher concentrations of the cytokine. However, most of these adherent cells down-regulated CD14 and stimulated the proliferation of a...lloreactive T cells. In contrast adherent cells cultivated with GM-CSF alone were predominantly macrophages as judged by the expression of CD14 and the inefficiency to stimulate alloreactive T cells. DC generated in the presence of lower concentrations of IL-4 had higher proapoptotic potential for the Jurkat cell line than DC differentiated with higher concentrations of IL-4, suggesting their stronger cytotoxic, anti-tumor effect.
U više laboratorija su uspostavljeni sistemi za kultivaciju velikog broja humanih dendritičnih ćelija (DĆ) od monocita korišćenjem faktora stimulacije granulocitno--makrofagnih kolonija (GM-CSF) i interleukina-4 (IL-4). U ovom radu je pokazano da je kombinacija GM-CSF (100 ng/ml) i mala koncentracija IL-4 (5 ng/ml) podjednako efikasna za dobijanje nezrelih, neadherentnih, DĆ monocitnog porekla kao i kombinacija GM-CSF sa deset puta većom koncentracijom IL-4 (50 ng/ml). Ovaj zaključak izveden je na osnovu sličnog fenotipskog profila DĆ (ispoljavanje CD1a, CD80, CD86 i HLA-DR, smanjenje ekspresije CD14 i odsustva CD83), kao i slične alostimulatorne aktivnosti ovih ćelija za limfocite T. U kulturama sa nižim koncentracijama IL-4 prisutan je bio veći broj adherentnih ćelija nego u kulturama sa većim koncentracijama IL-4. Međutim, većina ovih ćelija je smanjivala ekspresiju CD14 i stimulisala proliferaciju aloreaktivnih limfocita T. Nasuprot njima adherentne ćelije, diferentovane samo u pri...sustvu GM-CSF, koje su ispoljavale CD14 i nisu imale sposobnost stimulacije aloreakativnih limfocita T pokazivale su karakteristike makrofaga. DĆ obrazovane u prisustvu manjih koncentracija IL-4 imale su veći potencijal za indukciju apoptoze Jurkat ćelijske linije, a time i snažniji citotoksični, antitumorski efekat nego DĆ diferentovane u prisustvu većih koncentracija IL-4.
Кључне речи:
dendritic cells / monocytes / phenotype / granulocyte-macrophage colony-stimulating factor / interleukin-4 / apoptosis / T-lymphocytes / ćelije / dendritične / monociti / fenotip / faktor stimulacije granulocitno-makrofagne kolonije / interleukin-4 / apoptoza / limfociti TИзвор:
Vojnosanitetski pregled, 2003, 60, 5, 531-538Издавач:
- Vojnomedicinska akademija - Institut za naučne informacije, Beograd
Институција/група
PharmacyTY - JOUR AU - Čolić, Miodrag AU - Jandrić, Dušan AU - Stojić-Vukanić, Zorica AU - Antić-Stanković, Jelena AU - Popović, Petar AU - Vasilijić, Saša AU - Milosavljević, Petar AU - Balint, Bela PY - 2003 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/465 AB - Several laboratories have developed culture systems that allow the generation of large numbers of human dendritic cells (DC) from monocytes using granulocyte-macrophage colony stimulating factor (GM-CSF), and interleukin-4 (IL-4). In this work we provided evidence that GM-CSF (100 ng/ml) in combination with a low concentration of IL-4 (5 ng/ml) was efficient in the generation of immature, non-adherent, monocyte-derived DC as the same concentration of GM-CSF, and ten times higher concentration of IL-4 (50 ng/ml). This conclusion was based on the similar phenotype profile of DC such as the expression of CD1a, CD80, CD86, and HLA-DR, down-regulation of CD14, and the absence of CD83, as well as on their similar allostimulatory activity for T cells. A higher number of cells remained adherent in cultures with lower concentrations of IL-4 than in cultures with higher concentrations of the cytokine. However, most of these adherent cells down-regulated CD14 and stimulated the proliferation of alloreactive T cells. In contrast adherent cells cultivated with GM-CSF alone were predominantly macrophages as judged by the expression of CD14 and the inefficiency to stimulate alloreactive T cells. DC generated in the presence of lower concentrations of IL-4 had higher proapoptotic potential for the Jurkat cell line than DC differentiated with higher concentrations of IL-4, suggesting their stronger cytotoxic, anti-tumor effect. AB - U više laboratorija su uspostavljeni sistemi za kultivaciju velikog broja humanih dendritičnih ćelija (DĆ) od monocita korišćenjem faktora stimulacije granulocitno--makrofagnih kolonija (GM-CSF) i interleukina-4 (IL-4). U ovom radu je pokazano da je kombinacija GM-CSF (100 ng/ml) i mala koncentracija IL-4 (5 ng/ml) podjednako efikasna za dobijanje nezrelih, neadherentnih, DĆ monocitnog porekla kao i kombinacija GM-CSF sa deset puta većom koncentracijom IL-4 (50 ng/ml). Ovaj zaključak izveden je na osnovu sličnog fenotipskog profila DĆ (ispoljavanje CD1a, CD80, CD86 i HLA-DR, smanjenje ekspresije CD14 i odsustva CD83), kao i slične alostimulatorne aktivnosti ovih ćelija za limfocite T. U kulturama sa nižim koncentracijama IL-4 prisutan je bio veći broj adherentnih ćelija nego u kulturama sa većim koncentracijama IL-4. Međutim, većina ovih ćelija je smanjivala ekspresiju CD14 i stimulisala proliferaciju aloreaktivnih limfocita T. Nasuprot njima adherentne ćelije, diferentovane samo u prisustvu GM-CSF, koje su ispoljavale CD14 i nisu imale sposobnost stimulacije aloreakativnih limfocita T pokazivale su karakteristike makrofaga. DĆ obrazovane u prisustvu manjih koncentracija IL-4 imale su veći potencijal za indukciju apoptoze Jurkat ćelijske linije, a time i snažniji citotoksični, antitumorski efekat nego DĆ diferentovane u prisustvu većih koncentracija IL-4. PB - Vojnomedicinska akademija - Institut za naučne informacije, Beograd T2 - Vojnosanitetski pregled T1 - Differentiation of human dendritic cells from monocytes in vitro using granulocyte-macrophage colony stimulating factor and low concentration of interleukin-4 T1 - Diferencijacija humanih dendritičnih ćelija od monocita in vitro korišćenjem faktora stimulacije granulocitno-makrofagnih kolonija i niske koncentracije interleukina-4 VL - 60 IS - 5 SP - 531 EP - 538 DO - 10.2298/VSP0305531C ER -
@article{ author = "Čolić, Miodrag and Jandrić, Dušan and Stojić-Vukanić, Zorica and Antić-Stanković, Jelena and Popović, Petar and Vasilijić, Saša and Milosavljević, Petar and Balint, Bela", year = "2003", abstract = "Several laboratories have developed culture systems that allow the generation of large numbers of human dendritic cells (DC) from monocytes using granulocyte-macrophage colony stimulating factor (GM-CSF), and interleukin-4 (IL-4). In this work we provided evidence that GM-CSF (100 ng/ml) in combination with a low concentration of IL-4 (5 ng/ml) was efficient in the generation of immature, non-adherent, monocyte-derived DC as the same concentration of GM-CSF, and ten times higher concentration of IL-4 (50 ng/ml). This conclusion was based on the similar phenotype profile of DC such as the expression of CD1a, CD80, CD86, and HLA-DR, down-regulation of CD14, and the absence of CD83, as well as on their similar allostimulatory activity for T cells. A higher number of cells remained adherent in cultures with lower concentrations of IL-4 than in cultures with higher concentrations of the cytokine. However, most of these adherent cells down-regulated CD14 and stimulated the proliferation of alloreactive T cells. In contrast adherent cells cultivated with GM-CSF alone were predominantly macrophages as judged by the expression of CD14 and the inefficiency to stimulate alloreactive T cells. DC generated in the presence of lower concentrations of IL-4 had higher proapoptotic potential for the Jurkat cell line than DC differentiated with higher concentrations of IL-4, suggesting their stronger cytotoxic, anti-tumor effect., U više laboratorija su uspostavljeni sistemi za kultivaciju velikog broja humanih dendritičnih ćelija (DĆ) od monocita korišćenjem faktora stimulacije granulocitno--makrofagnih kolonija (GM-CSF) i interleukina-4 (IL-4). U ovom radu je pokazano da je kombinacija GM-CSF (100 ng/ml) i mala koncentracija IL-4 (5 ng/ml) podjednako efikasna za dobijanje nezrelih, neadherentnih, DĆ monocitnog porekla kao i kombinacija GM-CSF sa deset puta većom koncentracijom IL-4 (50 ng/ml). Ovaj zaključak izveden je na osnovu sličnog fenotipskog profila DĆ (ispoljavanje CD1a, CD80, CD86 i HLA-DR, smanjenje ekspresije CD14 i odsustva CD83), kao i slične alostimulatorne aktivnosti ovih ćelija za limfocite T. U kulturama sa nižim koncentracijama IL-4 prisutan je bio veći broj adherentnih ćelija nego u kulturama sa većim koncentracijama IL-4. Međutim, većina ovih ćelija je smanjivala ekspresiju CD14 i stimulisala proliferaciju aloreaktivnih limfocita T. Nasuprot njima adherentne ćelije, diferentovane samo u prisustvu GM-CSF, koje su ispoljavale CD14 i nisu imale sposobnost stimulacije aloreakativnih limfocita T pokazivale su karakteristike makrofaga. DĆ obrazovane u prisustvu manjih koncentracija IL-4 imale su veći potencijal za indukciju apoptoze Jurkat ćelijske linije, a time i snažniji citotoksični, antitumorski efekat nego DĆ diferentovane u prisustvu većih koncentracija IL-4.", publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd", journal = "Vojnosanitetski pregled", title = "Differentiation of human dendritic cells from monocytes in vitro using granulocyte-macrophage colony stimulating factor and low concentration of interleukin-4, Diferencijacija humanih dendritičnih ćelija od monocita in vitro korišćenjem faktora stimulacije granulocitno-makrofagnih kolonija i niske koncentracije interleukina-4", volume = "60", number = "5", pages = "531-538", doi = "10.2298/VSP0305531C" }
Čolić, M., Jandrić, D., Stojić-Vukanić, Z., Antić-Stanković, J., Popović, P., Vasilijić, S., Milosavljević, P.,& Balint, B.. (2003). Differentiation of human dendritic cells from monocytes in vitro using granulocyte-macrophage colony stimulating factor and low concentration of interleukin-4. in Vojnosanitetski pregled Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 60(5), 531-538. https://doi.org/10.2298/VSP0305531C
Čolić M, Jandrić D, Stojić-Vukanić Z, Antić-Stanković J, Popović P, Vasilijić S, Milosavljević P, Balint B. Differentiation of human dendritic cells from monocytes in vitro using granulocyte-macrophage colony stimulating factor and low concentration of interleukin-4. in Vojnosanitetski pregled. 2003;60(5):531-538. doi:10.2298/VSP0305531C .
Čolić, Miodrag, Jandrić, Dušan, Stojić-Vukanić, Zorica, Antić-Stanković, Jelena, Popović, Petar, Vasilijić, Saša, Milosavljević, Petar, Balint, Bela, "Differentiation of human dendritic cells from monocytes in vitro using granulocyte-macrophage colony stimulating factor and low concentration of interleukin-4" in Vojnosanitetski pregled, 60, no. 5 (2003):531-538, https://doi.org/10.2298/VSP0305531C . .