Chemical composition and antimicrobial activity of volatile fraction of Anthriscus nemorosa (Bieb.) Sprengel (Umbelliferae)
Authors
Pavlović, MilicaPetrović, Silvana
Couladis, Maria
Tzakou, Olga
Milenković, Marina
Niketić, Marjan
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Anthriscus nemorosa (Bieb.) Sprengel (Umbelliferae) is a nitrophile pererurial with a long, stout root. This plant occurs in the temperate area of Eurasia [l], from ltaly on the west to Japan on the East. The species is a member of A. sylvestris (L.) Hoffm. complex [2]. In Serbia, it usually inhabits the shady herbaceous layer in thermophilous forests and underbrush vegetation at the lower altitudes. It prefers moderately wet, steep, stony and eroded slopes. From methanol extract of the roots of A. nemorosa two lignan lactones (savinin, and nemerosin) were isolated [3].
We investigated composition and antimicrobial activity of essential oil from the roots of this plant.
Plant material was collected in Sićevača gorge (E. Serbia), in July 2007. The essential oil was isolated by hydrodistillation and analyzed by GC and GC-MS. The roots yielded 0.2% w/w of light yellow oil with aromatic smell. The main constituents of the oil were: n-nonane (12.1%), n-hexadecanol (6.9%), δ-cadinene (6.4%...), β-pinene (6.0%) and germacrene D (5.4%). The essential oil from roots of A. nemorosa differed from the root oil of the closely related species A. sylvestris (L.) Hoffm. which was dominated by β-phellandrene (45.4%), Z-β-ocimene (16.9%), and α-pinene (4.6%) [4].
The microbial growth inhibitory properties of isolated essential oil were determined using the broth microdilution method [5] against Gram-positive bacteria Staphylococcus epidermidis (ATCC 12228), Bacillus subtilis (ATCC 6633), Gram-negative bacteria Escherichia coli (ATCC 25922) and a yeast
Candida albicans (ATCC 10259 and ATCC 24433). The best inhibitory effect was detected against B. subtilis (MIC 6.25 µg/ml) and C. albicans (ATCC 10259) (MIC 50 µg/ml).
References: [1] Nikolić, V. (1973): Anthriscus Pers. In: Josifović, M. (ed.): Flora SR Srbije 5: 334-338. Srpska akademija nauka i umetnosti, Beograd.; [2] Cannon, J.F.M. (1968): Anthriscus Pers. In: Tutin, T.G., Heywood, V.H., Burges, N.A., Moore, D.M., Valentine, D.H., Walters, S.M., Webb, D.A. (eds): Flora Europaea 2: 326. University Press, Cambridge.; [3] Turabelidze, D.G., Mikaya, G.A., Kemertelidze, Vul"fson, N.S. (1982) Bioorg. Khim. 8: 695-701.; [4] Bos, R., Koulman, A., Woerdenbag, H.J., Quax, W.J., Pras, N. (2002) J. Chromatogr. A 966: 233-238.; [5] Candan, F., Unlq M., Tepe, 8., Daferera, D., Polissiou, M., Sdkmen, A., Akpulat, A. (2003) J. Ethnopharmacol. 87: 215-220.
Keywords:
Anthriscus nemorosa / volatiles / GC and GC-MS / antimicrobial activitySource:
5th Balkan Botanical Congress, 07-11 September 2009, Belgrade, Serbia, Book of Abstracts, 2009, 147-Publisher:
- Faculty o f Biology, University of Belgrade
Funding / projects:
- Ispitivanje lekovitog potencijala biljaka: morfološka, hemijska i farmakološka karakterizacija (RS-MESTD-MPN2006-2010-143012)
Note:
- The 80th anniversary of the publication of Turrill’s “Plant life of the Balkan peninsula”
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Institution/Community
PharmacyTY - CONF AU - Pavlović, Milica AU - Petrović, Silvana AU - Couladis, Maria AU - Tzakou, Olga AU - Milenković, Marina AU - Niketić, Marjan PY - 2009 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5162 AB - Anthriscus nemorosa (Bieb.) Sprengel (Umbelliferae) is a nitrophile pererurial with a long, stout root. This plant occurs in the temperate area of Eurasia [l], from ltaly on the west to Japan on the East. The species is a member of A. sylvestris (L.) Hoffm. complex [2]. In Serbia, it usually inhabits the shady herbaceous layer in thermophilous forests and underbrush vegetation at the lower altitudes. It prefers moderately wet, steep, stony and eroded slopes. From methanol extract of the roots of A. nemorosa two lignan lactones (savinin, and nemerosin) were isolated [3]. We investigated composition and antimicrobial activity of essential oil from the roots of this plant. Plant material was collected in Sićevača gorge (E. Serbia), in July 2007. The essential oil was isolated by hydrodistillation and analyzed by GC and GC-MS. The roots yielded 0.2% w/w of light yellow oil with aromatic smell. The main constituents of the oil were: n-nonane (12.1%), n-hexadecanol (6.9%), δ-cadinene (6.4%), β-pinene (6.0%) and germacrene D (5.4%). The essential oil from roots of A. nemorosa differed from the root oil of the closely related species A. sylvestris (L.) Hoffm. which was dominated by β-phellandrene (45.4%), Z-β-ocimene (16.9%), and α-pinene (4.6%) [4]. The microbial growth inhibitory properties of isolated essential oil were determined using the broth microdilution method [5] against Gram-positive bacteria Staphylococcus epidermidis (ATCC 12228), Bacillus subtilis (ATCC 6633), Gram-negative bacteria Escherichia coli (ATCC 25922) and a yeast Candida albicans (ATCC 10259 and ATCC 24433). The best inhibitory effect was detected against B. subtilis (MIC 6.25 µg/ml) and C. albicans (ATCC 10259) (MIC 50 µg/ml). References: [1] Nikolić, V. (1973): Anthriscus Pers. In: Josifović, M. (ed.): Flora SR Srbije 5: 334-338. Srpska akademija nauka i umetnosti, Beograd.; [2] Cannon, J.F.M. (1968): Anthriscus Pers. In: Tutin, T.G., Heywood, V.H., Burges, N.A., Moore, D.M., Valentine, D.H., Walters, S.M., Webb, D.A. (eds): Flora Europaea 2: 326. University Press, Cambridge.; [3] Turabelidze, D.G., Mikaya, G.A., Kemertelidze, Vul"fson, N.S. (1982) Bioorg. Khim. 8: 695-701.; [4] Bos, R., Koulman, A., Woerdenbag, H.J., Quax, W.J., Pras, N. (2002) J. Chromatogr. A 966: 233-238.; [5] Candan, F., Unlq M., Tepe, 8., Daferera, D., Polissiou, M., Sdkmen, A., Akpulat, A. (2003) J. Ethnopharmacol. 87: 215-220. PB - Faculty o f Biology, University of Belgrade C3 - 5th Balkan Botanical Congress, 07-11 September 2009, Belgrade, Serbia, Book of Abstracts T1 - Chemical composition and antimicrobial activity of volatile fraction of Anthriscus nemorosa (Bieb.) Sprengel (Umbelliferae) SP - 147 UR - https://hdl.handle.net/21.15107/rcub_farfar_5162 ER -
@conference{ author = "Pavlović, Milica and Petrović, Silvana and Couladis, Maria and Tzakou, Olga and Milenković, Marina and Niketić, Marjan", year = "2009", abstract = "Anthriscus nemorosa (Bieb.) Sprengel (Umbelliferae) is a nitrophile pererurial with a long, stout root. This plant occurs in the temperate area of Eurasia [l], from ltaly on the west to Japan on the East. The species is a member of A. sylvestris (L.) Hoffm. complex [2]. In Serbia, it usually inhabits the shady herbaceous layer in thermophilous forests and underbrush vegetation at the lower altitudes. It prefers moderately wet, steep, stony and eroded slopes. From methanol extract of the roots of A. nemorosa two lignan lactones (savinin, and nemerosin) were isolated [3]. We investigated composition and antimicrobial activity of essential oil from the roots of this plant. Plant material was collected in Sićevača gorge (E. Serbia), in July 2007. The essential oil was isolated by hydrodistillation and analyzed by GC and GC-MS. The roots yielded 0.2% w/w of light yellow oil with aromatic smell. The main constituents of the oil were: n-nonane (12.1%), n-hexadecanol (6.9%), δ-cadinene (6.4%), β-pinene (6.0%) and germacrene D (5.4%). The essential oil from roots of A. nemorosa differed from the root oil of the closely related species A. sylvestris (L.) Hoffm. which was dominated by β-phellandrene (45.4%), Z-β-ocimene (16.9%), and α-pinene (4.6%) [4]. The microbial growth inhibitory properties of isolated essential oil were determined using the broth microdilution method [5] against Gram-positive bacteria Staphylococcus epidermidis (ATCC 12228), Bacillus subtilis (ATCC 6633), Gram-negative bacteria Escherichia coli (ATCC 25922) and a yeast Candida albicans (ATCC 10259 and ATCC 24433). The best inhibitory effect was detected against B. subtilis (MIC 6.25 µg/ml) and C. albicans (ATCC 10259) (MIC 50 µg/ml). References: [1] Nikolić, V. (1973): Anthriscus Pers. In: Josifović, M. (ed.): Flora SR Srbije 5: 334-338. Srpska akademija nauka i umetnosti, Beograd.; [2] Cannon, J.F.M. (1968): Anthriscus Pers. In: Tutin, T.G., Heywood, V.H., Burges, N.A., Moore, D.M., Valentine, D.H., Walters, S.M., Webb, D.A. (eds): Flora Europaea 2: 326. University Press, Cambridge.; [3] Turabelidze, D.G., Mikaya, G.A., Kemertelidze, Vul"fson, N.S. (1982) Bioorg. Khim. 8: 695-701.; [4] Bos, R., Koulman, A., Woerdenbag, H.J., Quax, W.J., Pras, N. (2002) J. Chromatogr. A 966: 233-238.; [5] Candan, F., Unlq M., Tepe, 8., Daferera, D., Polissiou, M., Sdkmen, A., Akpulat, A. (2003) J. Ethnopharmacol. 87: 215-220.", publisher = "Faculty o f Biology, University of Belgrade", journal = "5th Balkan Botanical Congress, 07-11 September 2009, Belgrade, Serbia, Book of Abstracts", title = "Chemical composition and antimicrobial activity of volatile fraction of Anthriscus nemorosa (Bieb.) Sprengel (Umbelliferae)", pages = "147", url = "https://hdl.handle.net/21.15107/rcub_farfar_5162" }
Pavlović, M., Petrović, S., Couladis, M., Tzakou, O., Milenković, M.,& Niketić, M.. (2009). Chemical composition and antimicrobial activity of volatile fraction of Anthriscus nemorosa (Bieb.) Sprengel (Umbelliferae). in 5th Balkan Botanical Congress, 07-11 September 2009, Belgrade, Serbia, Book of Abstracts Faculty o f Biology, University of Belgrade., 147. https://hdl.handle.net/21.15107/rcub_farfar_5162
Pavlović M, Petrović S, Couladis M, Tzakou O, Milenković M, Niketić M. Chemical composition and antimicrobial activity of volatile fraction of Anthriscus nemorosa (Bieb.) Sprengel (Umbelliferae). in 5th Balkan Botanical Congress, 07-11 September 2009, Belgrade, Serbia, Book of Abstracts. 2009;:147. https://hdl.handle.net/21.15107/rcub_farfar_5162 .
Pavlović, Milica, Petrović, Silvana, Couladis, Maria, Tzakou, Olga, Milenković, Marina, Niketić, Marjan, "Chemical composition and antimicrobial activity of volatile fraction of Anthriscus nemorosa (Bieb.) Sprengel (Umbelliferae)" in 5th Balkan Botanical Congress, 07-11 September 2009, Belgrade, Serbia, Book of Abstracts (2009):147, https://hdl.handle.net/21.15107/rcub_farfar_5162 .