TY  - JOUR
AU  - Okuka, Nina
AU  - Schuh, Verena
AU  - Krammer, Ulrike
AU  - Polovina, Snežana
AU  - Šumarac-Dumanović, Mirjana
AU  - Milinković, Neda
AU  - Veličković, Ksenija
AU  - Đorđević, Brižita
AU  - Haslberger, Alexander
AU  - Ivanović, Nevena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5054
AB  - Several studies report the important role of an altered gut microbiota in the development of obesity, highlighting the potential use of probiotics in the treatment of obesity. The aim of this study is to investigate the effect of a novel probiotic approach on the expression of specific miRNAs and mRNAs associated with obesity in combination with the hypocholesterolemic octacosanol. Twenty overweight/obese women participated in a randomized, placebo-controlled, double-blind study and were randomly divided into two groups: the intervention group (daily one capsule containing Lactobacillus plantarum 299v (DSM9843), Saccharomyces cerevisiae var. boulardii, and 40 mg octacosanol; N = 12) and the placebo group (N = 8). Changes in lipid parameters and expression of miRNAs and mRNAs were assessed before (T0) and after the 12-week intervention (T1). After the intervention, the expression of miR-155-5p (9.38 ± 0.85 vs. 8.38 ± 1.06, p = 0.05) and miR-24-3p (3.42 ± 0.38 vs. 2.71 ± 0.97, p = 0.031) showed significant decreases in the intervention group when compared to the control group. At T1, the expression of miR-155-5p (8.69 ± 1.31 vs. 9.3 ± 0.85, p = 0.04), miR-125b-5p (5.41 ± 1.18 vs. 5.99 ± 1.36, p = 0.049), and TNF-α (10.24 ± 1.66 vs. 11.36 ± 1.12, p = 0.009) were significantly decreased in the intervention group. No changes in lipids and anthropometric parameters were observed. The novel probiotic approach had a positive effect on regulating the expression of certain miRNAs and mRNAs important for regulating inflammation and adipogenesis, which are essential for obesity onset and control.
PB  - MDPI
T2  - Life
T1  - Epigenetic Aspects of a New Probiotic Concept—A Pilot Study
VL  - 13
IS  - 9
DO  - 10.3390/life13091912
ER  - 

@article{
author = "Okuka, Nina and Schuh, Verena and Krammer, Ulrike and Polovina, Snežana and Šumarac-Dumanović, Mirjana and Milinković, Neda and Veličković, Ksenija and Đorđević, Brižita and Haslberger, Alexander and Ivanović, Nevena",
year = "2023",
abstract = "Several studies report the important role of an altered gut microbiota in the development of obesity, highlighting the potential use of probiotics in the treatment of obesity. The aim of this study is to investigate the effect of a novel probiotic approach on the expression of specific miRNAs and mRNAs associated with obesity in combination with the hypocholesterolemic octacosanol. Twenty overweight/obese women participated in a randomized, placebo-controlled, double-blind study and were randomly divided into two groups: the intervention group (daily one capsule containing Lactobacillus plantarum 299v (DSM9843), Saccharomyces cerevisiae var. boulardii, and 40 mg octacosanol; N = 12) and the placebo group (N = 8). Changes in lipid parameters and expression of miRNAs and mRNAs were assessed before (T0) and after the 12-week intervention (T1). After the intervention, the expression of miR-155-5p (9.38 ± 0.85 vs. 8.38 ± 1.06, p = 0.05) and miR-24-3p (3.42 ± 0.38 vs. 2.71 ± 0.97, p = 0.031) showed significant decreases in the intervention group when compared to the control group. At T1, the expression of miR-155-5p (8.69 ± 1.31 vs. 9.3 ± 0.85, p = 0.04), miR-125b-5p (5.41 ± 1.18 vs. 5.99 ± 1.36, p = 0.049), and TNF-α (10.24 ± 1.66 vs. 11.36 ± 1.12, p = 0.009) were significantly decreased in the intervention group. No changes in lipids and anthropometric parameters were observed. The novel probiotic approach had a positive effect on regulating the expression of certain miRNAs and mRNAs important for regulating inflammation and adipogenesis, which are essential for obesity onset and control.",
publisher = "MDPI",
journal = "Life",
title = "Epigenetic Aspects of a New Probiotic Concept—A Pilot Study",
volume = "13",
number = "9",
doi = "10.3390/life13091912"
}

Okuka, N., Schuh, V., Krammer, U., Polovina, S., Šumarac-Dumanović, M., Milinković, N., Veličković, K., Đorđević, B., Haslberger, A.,& Ivanović, N.. (2023). Epigenetic Aspects of a New Probiotic Concept—A Pilot Study. in Life
MDPI., 13(9).
https://doi.org/10.3390/life13091912

Okuka N, Schuh V, Krammer U, Polovina S, Šumarac-Dumanović M, Milinković N, Veličković K, Đorđević B, Haslberger A, Ivanović N. Epigenetic Aspects of a New Probiotic Concept—A Pilot Study. in Life. 2023;13(9).
doi:10.3390/life13091912 .

Okuka, Nina, Schuh, Verena, Krammer, Ulrike, Polovina, Snežana, Šumarac-Dumanović, Mirjana, Milinković, Neda, Veličković, Ksenija, Đorđević, Brižita, Haslberger, Alexander, Ivanović, Nevena, "Epigenetic Aspects of a New Probiotic Concept—A Pilot Study" in Life, 13, no. 9 (2023),
https://doi.org/10.3390/life13091912 . .

TY  - CONF
AU  - Veličković, Ksenija
AU  - Okuka, Nina
AU  - Polovina, Snežana
AU  - Sumarac‐Dumanović, Mirjana
AU  - Ivanović, Nevena
AU  - Milinković, Neda
AU  - Đorđević, Brižita
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4590
AB  - Obesity could be associated with enhanced platelet (PL) function and thrombosis.
Also, changes in gut microbiota composition were observed in obese patients, and probiotics
supplementation has been shown to improve some of the obesity-associated metabolic
parameters. This study aimed to determine the possible effects of probiotic supplementation
on platelet morphology in overweight/obese premenopausal women.Twenty overweight
(BMI = 25.0-29.9 kg/m2) and obese (BMI ≥ 30.0 kg/m2) women participated in double-
blind, randomized, placebo-controlled study. They were randomly assigned to receive one
capsule daily of probiotics (7x1010 CFU Lactobacillus plantarum 299v (DSM9843), 5x109
CFU Saccharomyces cerevisiae var. boulardii and 40mg octacosanol; N=10) or placebo
(N=10) for 3 months. To determine changes in the parameters of platelet activation - the
platelet count (PC), platelet distribution width (PDW), Feret diameter, and hypogranular
appearance, blood samples were obtained for an automated hematology analyser and
peripheral smears staining. Although difference in PC was not found between groups,
supplementation with probiotics decreased PDW compared to the placebo group (p<0.05).
Regarding platelets diameter, there is an decrease in group supplemented with probiotics
compared to placebo, however, a statistical difference has not been reached. Further, the
proportion of hypogranular PL was increased in a probiotic-supplemented group compared
to the placebo group (p<0.01). In conclusion, probiotic supplementation achieved
improvements in platelets morphology and hematological indices in overweight/obese
premenopausal women.
AB  - Gojaznost je često povezana sa povećanom funkcijom krvnih pločica i trombozom.
Takođe, istraživanja ukazuju na promenjen sastav mikrobioma creva kod gojaznih osoba,
kao i da suplementacija određenim probioticima može imati pozitivan uticaj na telesnu
masu. Cilj rada je bio da se ispita uticaj suplementacije probiotskog preparata na morfološke
karakteristike krvnih pločica u randomizovanoj, dvostruko-slepoj, placebo-kontrolisanoj
studiji. U tu svrhu, regrutovano je 20 žena sa prekomernom težinom (BMI ≥ 25,0 kg/m2) koje
su podeljene u dve grupe. Prva grupa je pila dnevno jednu kapsulu probiotskog preparata
tokom tri meseca (kombinaciju soja Lactobacillus plantarum 299v (DSM9843),
Saccharomyces cerevisiae var. boulardii i 40 mg oktakozanola; N = 10), dok je druga grupa
bila placebo grupa. Određen je broj krvnih pločica, i njihova raspodela po volumenu (eng.
platelet distribution width, PDW), a zatim je posmatrana morfologija krvnih pločica i
izmeren njihov dijametar iz krvnih razmaza. Analiza je pokazala da su krvne pločice kod
gojaznih/žena sa prekomernom težinom uglavnom krupne, što je jedan od pokazatelja
njihove aktivnosti. U interventoj grupi nije zapažena promena u broju krvnih pločica, ali je
značajno smanjen PDW u poređenju sa placebo grupom (p < 0,05). Dalje, pokazano je da je
udeo hipogranularnih krvnih pločica bio povećan u poređenju sa placebo grupom (p < 0,01).
Ovi rezultati ukazuju na pozitivan efekat probiotika na morfološke karakteristike krvnih
pločica i mogućnost primene probiotika u regulaciji njihove aktivnosti u patološkim
stanjima.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Effects of probiotic supplementation on platelet morphology in overweight/obese woman
T1  - Efekti suplementacije probiotikom na morfologiju krvnih pločica gojaznih žena i žena sa prokomernom težinom
VL  - 72
IS  - 4 suplement
SP  - S509
EP  - S510
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4590
ER  - 

@conference{
author = "Veličković, Ksenija and Okuka, Nina and Polovina, Snežana and Sumarac‐Dumanović, Mirjana and Ivanović, Nevena and Milinković, Neda and Đorđević, Brižita",
year = "2022",
abstract = "Obesity could be associated with enhanced platelet (PL) function and thrombosis.
Also, changes in gut microbiota composition were observed in obese patients, and probiotics
supplementation has been shown to improve some of the obesity-associated metabolic
parameters. This study aimed to determine the possible effects of probiotic supplementation
on platelet morphology in overweight/obese premenopausal women.Twenty overweight
(BMI = 25.0-29.9 kg/m2) and obese (BMI ≥ 30.0 kg/m2) women participated in double-
blind, randomized, placebo-controlled study. They were randomly assigned to receive one
capsule daily of probiotics (7x1010 CFU Lactobacillus plantarum 299v (DSM9843), 5x109
CFU Saccharomyces cerevisiae var. boulardii and 40mg octacosanol; N=10) or placebo
(N=10) for 3 months. To determine changes in the parameters of platelet activation - the
platelet count (PC), platelet distribution width (PDW), Feret diameter, and hypogranular
appearance, blood samples were obtained for an automated hematology analyser and
peripheral smears staining. Although difference in PC was not found between groups,
supplementation with probiotics decreased PDW compared to the placebo group (p<0.05).
Regarding platelets diameter, there is an decrease in group supplemented with probiotics
compared to placebo, however, a statistical difference has not been reached. Further, the
proportion of hypogranular PL was increased in a probiotic-supplemented group compared
to the placebo group (p<0.01). In conclusion, probiotic supplementation achieved
improvements in platelets morphology and hematological indices in overweight/obese
premenopausal women., Gojaznost je često povezana sa povećanom funkcijom krvnih pločica i trombozom.
Takođe, istraživanja ukazuju na promenjen sastav mikrobioma creva kod gojaznih osoba,
kao i da suplementacija određenim probioticima može imati pozitivan uticaj na telesnu
masu. Cilj rada je bio da se ispita uticaj suplementacije probiotskog preparata na morfološke
karakteristike krvnih pločica u randomizovanoj, dvostruko-slepoj, placebo-kontrolisanoj
studiji. U tu svrhu, regrutovano je 20 žena sa prekomernom težinom (BMI ≥ 25,0 kg/m2) koje
su podeljene u dve grupe. Prva grupa je pila dnevno jednu kapsulu probiotskog preparata
tokom tri meseca (kombinaciju soja Lactobacillus plantarum 299v (DSM9843),
Saccharomyces cerevisiae var. boulardii i 40 mg oktakozanola; N = 10), dok je druga grupa
bila placebo grupa. Određen je broj krvnih pločica, i njihova raspodela po volumenu (eng.
platelet distribution width, PDW), a zatim je posmatrana morfologija krvnih pločica i
izmeren njihov dijametar iz krvnih razmaza. Analiza je pokazala da su krvne pločice kod
gojaznih/žena sa prekomernom težinom uglavnom krupne, što je jedan od pokazatelja
njihove aktivnosti. U interventoj grupi nije zapažena promena u broju krvnih pločica, ali je
značajno smanjen PDW u poređenju sa placebo grupom (p < 0,05). Dalje, pokazano je da je
udeo hipogranularnih krvnih pločica bio povećan u poređenju sa placebo grupom (p < 0,01).
Ovi rezultati ukazuju na pozitivan efekat probiotika na morfološke karakteristike krvnih
pločica i mogućnost primene probiotika u regulaciji njihove aktivnosti u patološkim
stanjima.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Effects of probiotic supplementation on platelet morphology in overweight/obese woman, Efekti suplementacije probiotikom na morfologiju krvnih pločica gojaznih žena i žena sa prokomernom težinom",
volume = "72",
number = "4 suplement",
pages = "S509-S510",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4590"
}

Veličković, K., Okuka, N., Polovina, S., Sumarac‐Dumanović, M., Ivanović, N., Milinković, N.,& Đorđević, B.. (2022). Effects of probiotic supplementation on platelet morphology in overweight/obese woman. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S509-S510.
https://hdl.handle.net/21.15107/rcub_farfar_4590

Veličković K, Okuka N, Polovina S, Sumarac‐Dumanović M, Ivanović N, Milinković N, Đorđević B. Effects of probiotic supplementation on platelet morphology in overweight/obese woman. in Arhiv za farmaciju. 2022;72(4 suplement):S509-S510.
https://hdl.handle.net/21.15107/rcub_farfar_4590 .

Veličković, Ksenija, Okuka, Nina, Polovina, Snežana, Sumarac‐Dumanović, Mirjana, Ivanović, Nevena, Milinković, Neda, Đorđević, Brižita, "Effects of probiotic supplementation on platelet morphology in overweight/obese woman" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S509-S510,
https://hdl.handle.net/21.15107/rcub_farfar_4590 .