TY  - JOUR
AU  - Dinić, Miroslav
AU  - Pecikoza, Uroš
AU  - Đokić, Jelena
AU  - Stepanović-Petrović, Radica
AU  - Milenković, Marina
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Begović, Jelena
AU  - Golić, Nataša
AU  - Lukić, Jovanka
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3152
AB  - The aim of this study was to test the potential of high molecular weight exopolysaccharide (EPS) produced by the putative probiotic strain Lactobacillus paraplantarum BGCG11 (EPS CG11) to alleviate inflammatory pain in Wistar rats. The EPS CG11 was isolated from bacterial surface and was subjected to Fourier-transform infrared spectroscopy (FTIR) and thermal analysis. FTIR spectra confirmed the polysaccharide structure of isolated sample, while the thermal methods revealed good thermal properties of the polymer. The antihyperalgesic and antiedematous effects of the EPS CG11 were examined in the rat model of inflammation induced by carrageenan injection in hind paw. The results showed that the intraperitoneal administration of EPS CG11 produced a significant decrease in pain sensations (mechanical hyperalgesia) and a paw swelling in a dose-dependent manner as it was measured using Von Frey anesthesiometer and plethysmometer, respectively. These effects were followed by a decreased expression of IL-1 beta and iNOS mRNAs in rat's paw tissue suggesting that the antihyperalgesic and antiedematous effects of the EPS CG11 are related to the suppression of inflammatory response. Additionally, we demonstrated that EPS CG11 exhibits immunosuppressive properties in the peritonitis model induced by carrageenan. Expression levels of pro-inflammatory mediators IL-1 beta, TNF-alpha and iNOS were decreased, together with the enhanced secretion of anti-inflammatory IL-10 and IL-6 cytokines, while neutrophil infiltration was not changed. To the best of our knowledge, this is the first study which reports an antihyperalgesic effect as the novel property of bacterial EPSs. Given the high demands of pharmaceutical industry for the replacement of commonly used analgesics due to numerous side effects, this study describes a promising natural compound for the future pharmacological testing in the area.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Pharmacology
T1  - Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats
VL  - 9
DO  - 10.3389/fphar.2018.00001
ER  - 

@article{
author = "Dinić, Miroslav and Pecikoza, Uroš and Đokić, Jelena and Stepanović-Petrović, Radica and Milenković, Marina and Stevanović, Magdalena and Filipović, Nenad and Begović, Jelena and Golić, Nataša and Lukić, Jovanka",
year = "2018",
abstract = "The aim of this study was to test the potential of high molecular weight exopolysaccharide (EPS) produced by the putative probiotic strain Lactobacillus paraplantarum BGCG11 (EPS CG11) to alleviate inflammatory pain in Wistar rats. The EPS CG11 was isolated from bacterial surface and was subjected to Fourier-transform infrared spectroscopy (FTIR) and thermal analysis. FTIR spectra confirmed the polysaccharide structure of isolated sample, while the thermal methods revealed good thermal properties of the polymer. The antihyperalgesic and antiedematous effects of the EPS CG11 were examined in the rat model of inflammation induced by carrageenan injection in hind paw. The results showed that the intraperitoneal administration of EPS CG11 produced a significant decrease in pain sensations (mechanical hyperalgesia) and a paw swelling in a dose-dependent manner as it was measured using Von Frey anesthesiometer and plethysmometer, respectively. These effects were followed by a decreased expression of IL-1 beta and iNOS mRNAs in rat's paw tissue suggesting that the antihyperalgesic and antiedematous effects of the EPS CG11 are related to the suppression of inflammatory response. Additionally, we demonstrated that EPS CG11 exhibits immunosuppressive properties in the peritonitis model induced by carrageenan. Expression levels of pro-inflammatory mediators IL-1 beta, TNF-alpha and iNOS were decreased, together with the enhanced secretion of anti-inflammatory IL-10 and IL-6 cytokines, while neutrophil infiltration was not changed. To the best of our knowledge, this is the first study which reports an antihyperalgesic effect as the novel property of bacterial EPSs. Given the high demands of pharmaceutical industry for the replacement of commonly used analgesics due to numerous side effects, this study describes a promising natural compound for the future pharmacological testing in the area.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Pharmacology",
title = "Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats",
volume = "9",
doi = "10.3389/fphar.2018.00001"
}

Dinić, M., Pecikoza, U., Đokić, J., Stepanović-Petrović, R., Milenković, M., Stevanović, M., Filipović, N., Begović, J., Golić, N.,& Lukić, J.. (2018). Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats. in Frontiers in Pharmacology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fphar.2018.00001

Dinić M, Pecikoza U, Đokić J, Stepanović-Petrović R, Milenković M, Stevanović M, Filipović N, Begović J, Golić N, Lukić J. Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats. in Frontiers in Pharmacology. 2018;9.
doi:10.3389/fphar.2018.00001 .

Dinić, Miroslav, Pecikoza, Uroš, Đokić, Jelena, Stepanović-Petrović, Radica, Milenković, Marina, Stevanović, Magdalena, Filipović, Nenad, Begović, Jelena, Golić, Nataša, Lukić, Jovanka, "Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats" in Frontiers in Pharmacology, 9 (2018),
https://doi.org/10.3389/fphar.2018.00001 . .

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Lukić, Jovanka
AU  - Đokić, Jelena
AU  - Milenković, Marina
AU  - Strahinić, Ivana
AU  - Golić, Nataša
AU  - Begović, Jelena
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2917
AB  - The aim of this study was to investigate the potential of postbiotics originated from Lactobacillus fermentum BGHV110 strain (HV110) to counteract acetaminophen (APAP)-induced hepatotoxicity in HepG2 cells. This strain was selected according to its autophagy inducing potential, based on previous studies reporting protective role of autophagy in APAP caused cellular damage. Cell viability was assessed using MTT and LDH assays, while autophagy was monitored by qPCR analysis of BECN1, Atg5, p62/SQSTM1, and PINK1 mRNA expression and by Western blot analysis of p62/SQSTM1 and lipidated LC3 accumulation. Our results showed that detrimental effect of APAP on cell viability was suppressed in the presence of HV110 which was linked with increased conversion of LC3 protein and p62/SQSTM1 protein degradation. Additionally, higher p62/SQSTM1 and PINK1 mRNA transcription were noticed in cells co-treated with APAP/HV110, simultaneously. In conclusion, this study suggests that HV110 enhances activation of PINK1-dependent autophagy in HepG2 cells and its eventual co-supplementation with APAP could be potentially used for alleviation of hepatotoxic side effects caused by APAP overdose.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity
VL  - 8
DO  - 10.3389/fmicb.2017.00594
ER  - 

@article{
author = "Dinić, Miroslav and Lukić, Jovanka and Đokić, Jelena and Milenković, Marina and Strahinić, Ivana and Golić, Nataša and Begović, Jelena",
year = "2017",
abstract = "The aim of this study was to investigate the potential of postbiotics originated from Lactobacillus fermentum BGHV110 strain (HV110) to counteract acetaminophen (APAP)-induced hepatotoxicity in HepG2 cells. This strain was selected according to its autophagy inducing potential, based on previous studies reporting protective role of autophagy in APAP caused cellular damage. Cell viability was assessed using MTT and LDH assays, while autophagy was monitored by qPCR analysis of BECN1, Atg5, p62/SQSTM1, and PINK1 mRNA expression and by Western blot analysis of p62/SQSTM1 and lipidated LC3 accumulation. Our results showed that detrimental effect of APAP on cell viability was suppressed in the presence of HV110 which was linked with increased conversion of LC3 protein and p62/SQSTM1 protein degradation. Additionally, higher p62/SQSTM1 and PINK1 mRNA transcription were noticed in cells co-treated with APAP/HV110, simultaneously. In conclusion, this study suggests that HV110 enhances activation of PINK1-dependent autophagy in HepG2 cells and its eventual co-supplementation with APAP could be potentially used for alleviation of hepatotoxic side effects caused by APAP overdose.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity",
volume = "8",
doi = "10.3389/fmicb.2017.00594"
}

Dinić, M., Lukić, J., Đokić, J., Milenković, M., Strahinić, I., Golić, N.,& Begović, J.. (2017). Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fmicb.2017.00594

Dinić M, Lukić J, Đokić J, Milenković M, Strahinić I, Golić N, Begović J. Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity. in Frontiers in Microbiology. 2017;8.
doi:10.3389/fmicb.2017.00594 .

Dinić, Miroslav, Lukić, Jovanka, Đokić, Jelena, Milenković, Marina, Strahinić, Ivana, Golić, Nataša, Begović, Jelena, "Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity" in Frontiers in Microbiology, 8 (2017),
https://doi.org/10.3389/fmicb.2017.00594 . .

TY  - JOUR
AU  - Kolundžić, Marina
AU  - Groznanić, Nađa
AU  - Stanojković, Tatjana
AU  - Milenković, Marina
AU  - Dinić, Miroslav
AU  - Golić, Nataša
AU  - Kojić, Milan
AU  - Kundaković, Tatjana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2755
AB  - Cyclohexane, dichloromethane, and methanol extracts of the medicinal mushroom Laetiporus sulphureus were tested for their antimicrobial, cytotoxic, and radical scavenging activities. Total polyphenol content was also determined. Regarding the amount of the phenolic compounds in the methanol extract, there were 14.1 +/- 0.6 mg gallic acid/g dry extract and a concentration of 0.66 +/- 0.15 mg/mL resulted in the removal of 50% of the DPPH radicals (half-maximal inhibitory concentration [IC50]) . The average minimum inhibitory concentrations (MICs) against a select panel of microorganisms were between 125 and 250 mu g/mL, and the methanolic extract significantly inhibited the growth of Staphylococcus aureus (MIC, 31.25 mu g/mL). Also, cyclohexane and dichloromethane extracts inhibited the growth of Helicobacter pylori (MIC, 62.5 mu g/mL). Cyclohexane and dichloromethane extracts showed higher antiproliferative action compared with the methanol extract. The lowest IC50 was induced on HeLa cells by dichloromethane extract (IC50,67.20 +/- 5.49 mu g/mL), followed by cyclohexane extract (IC50 75.91 +/- 1.57 mu g/mL). The tested cyclohexane and dichloromethane extracts possessed moderate antibacterial and significant cytotoxic activity against both malignant (HeLa and N87) and healthy (MRC5) cell lines. Methanol extracts exhibited promising anti-Helicobacter activity without cytotoxicity against N87 and MRC5 cell lines.
PB  - Begell House Inc, Danbury
T2  - International Journal of Medicinal Mushrooms
T1  - Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia
VL  - 18
IS  - 6
SP  - 469
EP  - 476
DO  - 10.1615/IntJMedMushrooms.v18.i6.10
ER  - 

@article{
author = "Kolundžić, Marina and Groznanić, Nađa and Stanojković, Tatjana and Milenković, Marina and Dinić, Miroslav and Golić, Nataša and Kojić, Milan and Kundaković, Tatjana",
year = "2016",
abstract = "Cyclohexane, dichloromethane, and methanol extracts of the medicinal mushroom Laetiporus sulphureus were tested for their antimicrobial, cytotoxic, and radical scavenging activities. Total polyphenol content was also determined. Regarding the amount of the phenolic compounds in the methanol extract, there were 14.1 +/- 0.6 mg gallic acid/g dry extract and a concentration of 0.66 +/- 0.15 mg/mL resulted in the removal of 50% of the DPPH radicals (half-maximal inhibitory concentration [IC50]) . The average minimum inhibitory concentrations (MICs) against a select panel of microorganisms were between 125 and 250 mu g/mL, and the methanolic extract significantly inhibited the growth of Staphylococcus aureus (MIC, 31.25 mu g/mL). Also, cyclohexane and dichloromethane extracts inhibited the growth of Helicobacter pylori (MIC, 62.5 mu g/mL). Cyclohexane and dichloromethane extracts showed higher antiproliferative action compared with the methanol extract. The lowest IC50 was induced on HeLa cells by dichloromethane extract (IC50,67.20 +/- 5.49 mu g/mL), followed by cyclohexane extract (IC50 75.91 +/- 1.57 mu g/mL). The tested cyclohexane and dichloromethane extracts possessed moderate antibacterial and significant cytotoxic activity against both malignant (HeLa and N87) and healthy (MRC5) cell lines. Methanol extracts exhibited promising anti-Helicobacter activity without cytotoxicity against N87 and MRC5 cell lines.",
publisher = "Begell House Inc, Danbury",
journal = "International Journal of Medicinal Mushrooms",
title = "Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia",
volume = "18",
number = "6",
pages = "469-476",
doi = "10.1615/IntJMedMushrooms.v18.i6.10"
}

Kolundžić, M., Groznanić, N., Stanojković, T., Milenković, M., Dinić, M., Golić, N., Kojić, M.,& Kundaković, T.. (2016). Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia. in International Journal of Medicinal Mushrooms
Begell House Inc, Danbury., 18(6), 469-476.
https://doi.org/10.1615/IntJMedMushrooms.v18.i6.10

Kolundžić M, Groznanić N, Stanojković T, Milenković M, Dinić M, Golić N, Kojić M, Kundaković T. Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia. in International Journal of Medicinal Mushrooms. 2016;18(6):469-476.
doi:10.1615/IntJMedMushrooms.v18.i6.10 .

Kolundžić, Marina, Groznanić, Nađa, Stanojković, Tatjana, Milenković, Marina, Dinić, Miroslav, Golić, Nataša, Kojić, Milan, Kundaković, Tatjana, "Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia" in International Journal of Medicinal Mushrooms, 18, no. 6 (2016):469-476,
https://doi.org/10.1615/IntJMedMushrooms.v18.i6.10 . .

TY  - JOUR
AU  - Krivokuća, Marija
AU  - Niketić, Marjan
AU  - Milenković, Marina
AU  - Golić, Nataša
AU  - Masia, Carla
AU  - Scaltrito, Maria Maddalena
AU  - Sisto, Franceska
AU  - Kundaković, Tatjana
PY  - 2015
UR  - [http://farfar.pharmacy.bg.ac.rs/handle/123456789/3537]
AB  - The present study evaluated the anti-Helicobacter pylori activity of Alchemilla glabra Neygenf. (A. sect. Alchemilla), A. monticola Opiz (A. sect. Plicatae S.E.
Fröhner), A. fissa Günther & Schummel (A. sect. Calycinae (Buser) Buser) and A. viridiflora Rothm. (A. sect. Calycinae), and identified ellagic acid and
quercetin-3-O-β-D-glucoside. Anti-H. pylori activity was tested against ten clinical isolates and one reference strain (ATCC 43504). The methanol extracts
were more active than the dichloromethane and cyclohexane extracts. The ranges of concentrations were between 4 μg/mL for methanol extracts of
A. viridiflora, A. glabra and A. monticola, and 256 μg/mL for cyclohexane extracts of A. viridiflora, A. glabra and A. fissa. The best overall activity was
obtained with A. monticola extracts. No significant difference was found in the ellagic acid contents of the methanol extracts of the tested Alchemilla species
(0.2-0.3 mg/mL), and anti-H. pylori activity was similar (4 -32 μg/mL). Ellagic acid exhibited strong activity at very low concentrations (0.125-0.5
μg/mL), while the second identified compound, quercetin-3-O-β-D-glucoside, was also very active in concentration of 2-16 μg/mL.
PB  - Natural Products Inc, Westerville
T2  - Natural Product Communications
T1  - Anti-Helicobacter pylori Activity of Four Alchemilla Species (Rosaceae)
VL  - 10
UR  - https://hdl.handle.net/21.15107/rcub_farfar_3537
ER  - 

@article{
author = "Krivokuća, Marija and Niketić, Marjan and Milenković, Marina and Golić, Nataša and Masia, Carla and Scaltrito, Maria Maddalena and Sisto, Franceska and Kundaković, Tatjana",
year = "2015",
abstract = "The present study evaluated the anti-Helicobacter pylori activity of Alchemilla glabra Neygenf. (A. sect. Alchemilla), A. monticola Opiz (A. sect. Plicatae S.E.
Fröhner), A. fissa Günther & Schummel (A. sect. Calycinae (Buser) Buser) and A. viridiflora Rothm. (A. sect. Calycinae), and identified ellagic acid and
quercetin-3-O-β-D-glucoside. Anti-H. pylori activity was tested against ten clinical isolates and one reference strain (ATCC 43504). The methanol extracts
were more active than the dichloromethane and cyclohexane extracts. The ranges of concentrations were between 4 μg/mL for methanol extracts of
A. viridiflora, A. glabra and A. monticola, and 256 μg/mL for cyclohexane extracts of A. viridiflora, A. glabra and A. fissa. The best overall activity was
obtained with A. monticola extracts. No significant difference was found in the ellagic acid contents of the methanol extracts of the tested Alchemilla species
(0.2-0.3 mg/mL), and anti-H. pylori activity was similar (4 -32 μg/mL). Ellagic acid exhibited strong activity at very low concentrations (0.125-0.5
μg/mL), while the second identified compound, quercetin-3-O-β-D-glucoside, was also very active in concentration of 2-16 μg/mL.",
publisher = "Natural Products Inc, Westerville",
journal = "Natural Product Communications",
title = "Anti-Helicobacter pylori Activity of Four Alchemilla Species (Rosaceae)",
volume = "10",
url = "https://hdl.handle.net/21.15107/rcub_farfar_3537"
}

Krivokuća, M., Niketić, M., Milenković, M., Golić, N., Masia, C., Scaltrito, M. M., Sisto, F.,& Kundaković, T.. (2015). Anti-Helicobacter pylori Activity of Four Alchemilla Species (Rosaceae). in Natural Product Communications
Natural Products Inc, Westerville., 10.
https://hdl.handle.net/21.15107/rcub_farfar_3537

Krivokuća M, Niketić M, Milenković M, Golić N, Masia C, Scaltrito MM, Sisto F, Kundaković T. Anti-Helicobacter pylori Activity of Four Alchemilla Species (Rosaceae). in Natural Product Communications. 2015;10.
https://hdl.handle.net/21.15107/rcub_farfar_3537 .

Krivokuća, Marija, Niketić, Marjan, Milenković, Marina, Golić, Nataša, Masia, Carla, Scaltrito, Maria Maddalena, Sisto, Franceska, Kundaković, Tatjana, "Anti-Helicobacter pylori Activity of Four Alchemilla Species (Rosaceae)" in Natural Product Communications, 10 (2015),
https://hdl.handle.net/21.15107/rcub_farfar_3537 .

TY  - JOUR
AU  - Krivokuća, Marija
AU  - Niketić, Marjan
AU  - Milenković, Marina
AU  - Golić, Nataša
AU  - Masia, Carla
AU  - Scaltrito, Maria Maddalena
AU  - Sisto, Francesca
AU  - Kundaković, Tatjana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2436
AB  - The present study evaluated the anti-Helicobacter pylori activity of Alchemilla glabra Neygenf. (A. sect. Alchemilla), A. monticola Opiz (A. sect. Plicatae S.E. Frohner), A. fissa Gunther & Schummel (A. sect. Calycinae (Buser) Buser) and A. viridiflora Rothm. (A. sect. Calycinae), and identified ellagic acid and quercetin-3-O-beta-D-glucoside. Anti-H. pylori activity was tested against ten clinical isolates and one reference strain (ATCC 43504). The methanol extracts were more active than the dichloromethane and cyclohexane extracts. The ranges of concentrations were between 4 mu g/mL for methanol extracts of A. viridiflora, A. glabra and A. monticola, and 256 mu g/mL for cyclohexane extracts of A. viridiflora, A. glabra and A. fissa. The best overall activity was obtained with A. monticola extracts. No significant difference was found in the ellagic acid contents of the methanol extracts of the tested Alchemilla species (0.2-0.3 mg/mL), and anti-H. pylori activity was similar (4-32 mu g/mL). Ellagic acid exhibited strong activity at very low concentrations (0.125-0.5 mu g/mL), while the second identified compound, quercetin-3-O-beta-D-glucoside, was also very active in concentration of 2-16 mu g/mL.
PB  - Natural Products Inc, Westerville
T2  - Natural Product Communications
T1  - Anti-Helicobacter pylori Activity of Four Alchemilla Species (Rosaceae)
VL  - 10
IS  - 8
SP  - 1369
EP  - 1371
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2436
ER  - 

@article{
author = "Krivokuća, Marija and Niketić, Marjan and Milenković, Marina and Golić, Nataša and Masia, Carla and Scaltrito, Maria Maddalena and Sisto, Francesca and Kundaković, Tatjana",
year = "2015",
abstract = "The present study evaluated the anti-Helicobacter pylori activity of Alchemilla glabra Neygenf. (A. sect. Alchemilla), A. monticola Opiz (A. sect. Plicatae S.E. Frohner), A. fissa Gunther & Schummel (A. sect. Calycinae (Buser) Buser) and A. viridiflora Rothm. (A. sect. Calycinae), and identified ellagic acid and quercetin-3-O-beta-D-glucoside. Anti-H. pylori activity was tested against ten clinical isolates and one reference strain (ATCC 43504). The methanol extracts were more active than the dichloromethane and cyclohexane extracts. The ranges of concentrations were between 4 mu g/mL for methanol extracts of A. viridiflora, A. glabra and A. monticola, and 256 mu g/mL for cyclohexane extracts of A. viridiflora, A. glabra and A. fissa. The best overall activity was obtained with A. monticola extracts. No significant difference was found in the ellagic acid contents of the methanol extracts of the tested Alchemilla species (0.2-0.3 mg/mL), and anti-H. pylori activity was similar (4-32 mu g/mL). Ellagic acid exhibited strong activity at very low concentrations (0.125-0.5 mu g/mL), while the second identified compound, quercetin-3-O-beta-D-glucoside, was also very active in concentration of 2-16 mu g/mL.",
publisher = "Natural Products Inc, Westerville",
journal = "Natural Product Communications",
title = "Anti-Helicobacter pylori Activity of Four Alchemilla Species (Rosaceae)",
volume = "10",
number = "8",
pages = "1369-1371",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2436"
}

Krivokuća, M., Niketić, M., Milenković, M., Golić, N., Masia, C., Scaltrito, M. M., Sisto, F.,& Kundaković, T.. (2015). Anti-Helicobacter pylori Activity of Four Alchemilla Species (Rosaceae). in Natural Product Communications
Natural Products Inc, Westerville., 10(8), 1369-1371.
https://hdl.handle.net/21.15107/rcub_farfar_2436

Krivokuća M, Niketić M, Milenković M, Golić N, Masia C, Scaltrito MM, Sisto F, Kundaković T. Anti-Helicobacter pylori Activity of Four Alchemilla Species (Rosaceae). in Natural Product Communications. 2015;10(8):1369-1371.
https://hdl.handle.net/21.15107/rcub_farfar_2436 .

Krivokuća, Marija, Niketić, Marjan, Milenković, Marina, Golić, Nataša, Masia, Carla, Scaltrito, Maria Maddalena, Sisto, Francesca, Kundaković, Tatjana, "Anti-Helicobacter pylori Activity of Four Alchemilla Species (Rosaceae)" in Natural Product Communications, 10, no. 8 (2015):1369-1371,
https://hdl.handle.net/21.15107/rcub_farfar_2436 .

TY  - JOUR
AU  - Brđanin, Stanislava
AU  - Bogdanović, Nemanja
AU  - Kolundžić, Marina
AU  - Milenković, Marina
AU  - Golić, Nataša
AU  - Kojić, Milan
AU  - Kundaković, Tatjana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2349
AB  - The commercial samples of aerial parts of Origanum vulgare L. and Ocimum basilicum L. (Lamiaceae) were tested for antimicrobial activity. The activity of the extracts with different polarity was tested against a panel of microorganisms, including laboratory strain Helicobacter pylori NCTC 12868. The tested extracts showed a moderate activity. The extracts of O. vulgare were more active against bacteria, especially against Gram positive bacteria with the minimal inhibitory concentration (MIC) between 62.5 and 125 μg/mL, than the extracts of O. basilicum which were active against Candida albicans (MIC 125 μg/mL). Cyclohexane extract of O. vulgare did not show any activity against tested H. pylori, while all other tested extracts were active with MICs between 250 μg/mL and 500 μg/mL. Identified and quantified rosmarinic acid and other polar compounds could be active antibacterial compounds in these spices.
AB  - Komercijalni uzorci nadzemnih delova Origanum vulgare L. i Ocimum basilicum L. (Lamiaceae) su korišćeni za određivanje antimikrobne aktivnosti. Aktivnost ekstrakata različite polarnosti je testirana protiv izabranih mikroorganizama, uključujući laboratorijski soj Helicobacter pilori NCTC 12868. Testirani ekstrakti su imali umerenu aktivnost. Ekstrakti O. vulgare su bili aktivniji protiv bakterija, posebno Gram pozitivnih bakterija sa minimalnim inhibitornim koncentracijama (MIK) između 62,5 i 125 μg/mL, od ekstrakata O. basilicum koji su pokazali aktivnost protiv Candida albicans (MIK 125 μg/mL). Cikloheksanski ekstrakt O. vulgare nije pokazao antimikrobnu aktivnost na testirani H. pylori, dok su ostali testirani ekstrakti bili aktivni sa MIK vrednostima između 250 μg/mL i 500 μg/mL. Identifikovana i kvantifikovana ruzmarinska kiselina i druga polarna jedinjenja mogu predstavljati aktivna jedinjenja sa antibakterijskom aktivnošću u ovim začinima.
PB  - Univerzitet u Nišu - Tehnološki fakultet, Leskovac
T2  - Advanced Technologies
T1  - Antimicrobial activity of oregano (Origanum vulgare L.): And basil (Ocimum basilicum L.): Extracts
T1  - Antimikrobna aktivnost ekstrakata origana (Origanum vulgare L.) i bosiljka (Ocimum basilicum L.)
VL  - 4
IS  - 2
SP  - 5
EP  - 10
DO  - 10.5937/savteh1502005B
ER  - 

@article{
author = "Brđanin, Stanislava and Bogdanović, Nemanja and Kolundžić, Marina and Milenković, Marina and Golić, Nataša and Kojić, Milan and Kundaković, Tatjana",
year = "2015",
abstract = "The commercial samples of aerial parts of Origanum vulgare L. and Ocimum basilicum L. (Lamiaceae) were tested for antimicrobial activity. The activity of the extracts with different polarity was tested against a panel of microorganisms, including laboratory strain Helicobacter pylori NCTC 12868. The tested extracts showed a moderate activity. The extracts of O. vulgare were more active against bacteria, especially against Gram positive bacteria with the minimal inhibitory concentration (MIC) between 62.5 and 125 μg/mL, than the extracts of O. basilicum which were active against Candida albicans (MIC 125 μg/mL). Cyclohexane extract of O. vulgare did not show any activity against tested H. pylori, while all other tested extracts were active with MICs between 250 μg/mL and 500 μg/mL. Identified and quantified rosmarinic acid and other polar compounds could be active antibacterial compounds in these spices., Komercijalni uzorci nadzemnih delova Origanum vulgare L. i Ocimum basilicum L. (Lamiaceae) su korišćeni za određivanje antimikrobne aktivnosti. Aktivnost ekstrakata različite polarnosti je testirana protiv izabranih mikroorganizama, uključujući laboratorijski soj Helicobacter pilori NCTC 12868. Testirani ekstrakti su imali umerenu aktivnost. Ekstrakti O. vulgare su bili aktivniji protiv bakterija, posebno Gram pozitivnih bakterija sa minimalnim inhibitornim koncentracijama (MIK) između 62,5 i 125 μg/mL, od ekstrakata O. basilicum koji su pokazali aktivnost protiv Candida albicans (MIK 125 μg/mL). Cikloheksanski ekstrakt O. vulgare nije pokazao antimikrobnu aktivnost na testirani H. pylori, dok su ostali testirani ekstrakti bili aktivni sa MIK vrednostima između 250 μg/mL i 500 μg/mL. Identifikovana i kvantifikovana ruzmarinska kiselina i druga polarna jedinjenja mogu predstavljati aktivna jedinjenja sa antibakterijskom aktivnošću u ovim začinima.",
publisher = "Univerzitet u Nišu - Tehnološki fakultet, Leskovac",
journal = "Advanced Technologies",
title = "Antimicrobial activity of oregano (Origanum vulgare L.): And basil (Ocimum basilicum L.): Extracts, Antimikrobna aktivnost ekstrakata origana (Origanum vulgare L.) i bosiljka (Ocimum basilicum L.)",
volume = "4",
number = "2",
pages = "5-10",
doi = "10.5937/savteh1502005B"
}

Brđanin, S., Bogdanović, N., Kolundžić, M., Milenković, M., Golić, N., Kojić, M.,& Kundaković, T.. (2015). Antimicrobial activity of oregano (Origanum vulgare L.): And basil (Ocimum basilicum L.): Extracts. in Advanced Technologies
Univerzitet u Nišu - Tehnološki fakultet, Leskovac., 4(2), 5-10.
https://doi.org/10.5937/savteh1502005B

Brđanin S, Bogdanović N, Kolundžić M, Milenković M, Golić N, Kojić M, Kundaković T. Antimicrobial activity of oregano (Origanum vulgare L.): And basil (Ocimum basilicum L.): Extracts. in Advanced Technologies. 2015;4(2):5-10.
doi:10.5937/savteh1502005B .

Brđanin, Stanislava, Bogdanović, Nemanja, Kolundžić, Marina, Milenković, Marina, Golić, Nataša, Kojić, Milan, Kundaković, Tatjana, "Antimicrobial activity of oregano (Origanum vulgare L.): And basil (Ocimum basilicum L.): Extracts" in Advanced Technologies, 4, no. 2 (2015):5-10,
https://doi.org/10.5937/savteh1502005B . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Veljović, Katarina
AU  - Begović, Jelena
AU  - Filipić, Brankica
AU  - Popović, Dušanka
AU  - Tolinacki, Maja
AU  - Miljković, Marija
AU  - Kojić, Milan
AU  - Golić, Nataša
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2320
AB  - Enterococci represent the most controversial group of dairy bacteria. They are found to be the main constituent of many traditional Mediterranean dairy products and contribute to their characteristic taste and flavor. On the other hand, during the last 50 years antibiotic resistant enterococci have emerged as leading causes of nosocomial infections worldwide. The aim of this study was to determine the diversity, technological properties, antibiotic susceptibility and virulence traits of 636 enterococci previously isolated from 55 artisan dairy products from 12 locations in the Western Balkan countries (WBC) of Serbia, Croatia and Bosnia and Herzegovina. All strains were identified both by microbiological and molecular methods. The predominant species was Enterococcus durans, followed by Enterococcus faecalis and Enterococcus faecium. Over 44% of the isolates were resistant to ciprofloxacin and erythromycin, while 26.2% of the isolates were multi resistant to three or more antibiotics belonging to different families. 185 isolates (29.1%) were susceptible to all 13 of the antibiotics tested. The antibiotic-susceptible isolates were further tested for possible virulence genes and the production of biogenic amines. Finally, five enterococci isolates were found to be antibiotic susceptible with good technological characteristics and without virulence traits or the ability to produce biogenic amines, making them possible candidates for biotechnological application as starter cultures in the dairy industry.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?
VL  - 6
DO  - 10.3389/fmicb.2015.00954
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Veljović, Katarina and Begović, Jelena and Filipić, Brankica and Popović, Dušanka and Tolinacki, Maja and Miljković, Marija and Kojić, Milan and Golić, Nataša",
year = "2015",
abstract = "Enterococci represent the most controversial group of dairy bacteria. They are found to be the main constituent of many traditional Mediterranean dairy products and contribute to their characteristic taste and flavor. On the other hand, during the last 50 years antibiotic resistant enterococci have emerged as leading causes of nosocomial infections worldwide. The aim of this study was to determine the diversity, technological properties, antibiotic susceptibility and virulence traits of 636 enterococci previously isolated from 55 artisan dairy products from 12 locations in the Western Balkan countries (WBC) of Serbia, Croatia and Bosnia and Herzegovina. All strains were identified both by microbiological and molecular methods. The predominant species was Enterococcus durans, followed by Enterococcus faecalis and Enterococcus faecium. Over 44% of the isolates were resistant to ciprofloxacin and erythromycin, while 26.2% of the isolates were multi resistant to three or more antibiotics belonging to different families. 185 isolates (29.1%) were susceptible to all 13 of the antibiotics tested. The antibiotic-susceptible isolates were further tested for possible virulence genes and the production of biogenic amines. Finally, five enterococci isolates were found to be antibiotic susceptible with good technological characteristics and without virulence traits or the ability to produce biogenic amines, making them possible candidates for biotechnological application as starter cultures in the dairy industry.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?",
volume = "6",
doi = "10.3389/fmicb.2015.00954"
}

Terzić-Vidojević, A., Veljović, K., Begović, J., Filipić, B., Popović, D., Tolinacki, M., Miljković, M., Kojić, M.,& Golić, N.. (2015). Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 6.
https://doi.org/10.3389/fmicb.2015.00954

Terzić-Vidojević A, Veljović K, Begović J, Filipić B, Popović D, Tolinacki M, Miljković M, Kojić M, Golić N. Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?. in Frontiers in Microbiology. 2015;6.
doi:10.3389/fmicb.2015.00954 .

Terzić-Vidojević, Amarela, Veljović, Katarina, Begović, Jelena, Filipić, Brankica, Popović, Dušanka, Tolinacki, Maja, Miljković, Marija, Kojić, Milan, Golić, Nataša, "Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?" in Frontiers in Microbiology, 6 (2015),
https://doi.org/10.3389/fmicb.2015.00954 . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Golić, Nataša
AU  - Jovčić, Branko
AU  - Ćupić-Miladinović, Dejana
AU  - Soković, Svetlana
AU  - Popović, Dušanka
AU  - Kojić, Milan
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2483
AB  - Lactic acid bacteria (LAB) are widely used in the food industry, especially in the pro­duction of fermented dairy products and meat. The most studied species among LAB is Lactococcus lactis. L. lactis strains are of great importance in the production of fermented dairy products such as yogurt, butter, fresh cheese and some kind of semi-hard cheese. Although L. lactis acquired the 'Generally Regarded As Safe' (GRAS) status, many investigations indicated that lactococci may act as reservoirs of antibiotic resistance genes, which could be transferred to other bacterial species in human gastrointestinal tract includ­ing pathogens. The genome analysis of L. lactis indicated the presence of at least 40 putative drug transporter genes, and only four multidrug resistance (MDR) transporters are functional­ly characterized: LmrA, LmrP, LmrCD i CmbT. LmrA is the first described MDR transport­er in prokaryotes. LmrCD is responsible for resistance to cholate, which is an integral part of human bile and LmrCD is important for intestinal survival of lactococci that are used as probiotics. Secondary multidrug transporter LmrP confers resistance to lincosamides, mac­rolides, streptogramins and tetracyclines. CmbT protein has an effect on the host cell re­sistance to lincomycin, sulfadiazine, streptomycin, rifampicin, puromycin and sulfametox­azole. Since the food chain is an important way of transmitting resistance genes in human and animal population, it is of great importance to study the mechanisms of resistance in lactococci and other LAB, intended for the food industry.
AB  - Bakterije mlečne kiseline (BMK) imaju veliku primenu u prehrambenoj industriji, posebno u proizvodnji fermentisanih mlečnih proizvoda i mesa. Najviše izučavana vrsta u okviru BMK je Lactococcus lactis. Sojevi koji pripadaju vrsti L. lactis su od izuzetnog značaja u proizvodnji fermentisanih mlečnih proizvoda poput kiselog mleka, maslaca, svežih sireva i nekih vrsta polutvrdih sireva. Iako se generalno laktokoke smatraju bezbednim mikroorganizmima, istraživanja ukazuju da mogu biti rezervoari gena za rezistenciju na antibiotike, koji se horizontalnim transferom mogu preneti na druge bakterijske vrste, uključujući i patogene. Pokazano je da se u membrani bakterija vrste L. lactis nalaze efluksne pumpe koje obezbeđuju rezistenciju na strukturno različite supstrate (eng. multidrug resistance transporters-MDR). Analizom genoma L. lactis detektovano je prisustvo 40 potencijalnih gena koji kodiraju MDR transportere, od čega su do danas svega četiri funkcionalno okarakterisana: LmrA, LmrP, LmrCD i CmbT. LmrA je prvi uopšte opisan primarni transporter kod prokariota. LmrCD obezbeđuje rezistenciju na holat koji ulazi u sastav žuči, što je značajno za preživljavanje laktokoka koje se primenjuju kao probiotici u digestivnom traktu ljudi i životinja. LmrP je sekundarni transporter i obezbeđuje rezistenciju na neke linkozamide, makrolide, streptogramine I tetracikline. CmbT je poslednji okarakterisan MDR transporter kod L. lactis i doprinosi rezistenciji na linkomicin, sulfonamidne antibiotike, sulfametoksazol i sulfadiazin, aminoglikozidni antibiotik streptomicin, aminonukleozidni antibiotik puromicin i semisintetski antibiotik rifampicin. Kako lanac hrane predstavlja važan put prenosa gena za rezistenciju u okviru ljudske i životinjske populacije, neophodno je intenzivno izučavanje mehanizama rezistencije kod laktokoka i drugih BMK namenjenih za upotrebu u prehrambenoj industriji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Resistance to antibiotics in lacid acid bacteria: Strain Lactococcus
T1  - Rezistencija na antibiotike kod bakterija mlečne kiseline - rod Lactococcus
VL  - 69
IS  - 3-4
SP  - 271
EP  - 281
DO  - 10.2298/VETGL1504271F
ER  - 

@article{
author = "Filipić, Brankica and Golić, Nataša and Jovčić, Branko and Ćupić-Miladinović, Dejana and Soković, Svetlana and Popović, Dušanka and Kojić, Milan",
year = "2015",
abstract = "Lactic acid bacteria (LAB) are widely used in the food industry, especially in the pro­duction of fermented dairy products and meat. The most studied species among LAB is Lactococcus lactis. L. lactis strains are of great importance in the production of fermented dairy products such as yogurt, butter, fresh cheese and some kind of semi-hard cheese. Although L. lactis acquired the 'Generally Regarded As Safe' (GRAS) status, many investigations indicated that lactococci may act as reservoirs of antibiotic resistance genes, which could be transferred to other bacterial species in human gastrointestinal tract includ­ing pathogens. The genome analysis of L. lactis indicated the presence of at least 40 putative drug transporter genes, and only four multidrug resistance (MDR) transporters are functional­ly characterized: LmrA, LmrP, LmrCD i CmbT. LmrA is the first described MDR transport­er in prokaryotes. LmrCD is responsible for resistance to cholate, which is an integral part of human bile and LmrCD is important for intestinal survival of lactococci that are used as probiotics. Secondary multidrug transporter LmrP confers resistance to lincosamides, mac­rolides, streptogramins and tetracyclines. CmbT protein has an effect on the host cell re­sistance to lincomycin, sulfadiazine, streptomycin, rifampicin, puromycin and sulfametox­azole. Since the food chain is an important way of transmitting resistance genes in human and animal population, it is of great importance to study the mechanisms of resistance in lactococci and other LAB, intended for the food industry., Bakterije mlečne kiseline (BMK) imaju veliku primenu u prehrambenoj industriji, posebno u proizvodnji fermentisanih mlečnih proizvoda i mesa. Najviše izučavana vrsta u okviru BMK je Lactococcus lactis. Sojevi koji pripadaju vrsti L. lactis su od izuzetnog značaja u proizvodnji fermentisanih mlečnih proizvoda poput kiselog mleka, maslaca, svežih sireva i nekih vrsta polutvrdih sireva. Iako se generalno laktokoke smatraju bezbednim mikroorganizmima, istraživanja ukazuju da mogu biti rezervoari gena za rezistenciju na antibiotike, koji se horizontalnim transferom mogu preneti na druge bakterijske vrste, uključujući i patogene. Pokazano je da se u membrani bakterija vrste L. lactis nalaze efluksne pumpe koje obezbeđuju rezistenciju na strukturno različite supstrate (eng. multidrug resistance transporters-MDR). Analizom genoma L. lactis detektovano je prisustvo 40 potencijalnih gena koji kodiraju MDR transportere, od čega su do danas svega četiri funkcionalno okarakterisana: LmrA, LmrP, LmrCD i CmbT. LmrA je prvi uopšte opisan primarni transporter kod prokariota. LmrCD obezbeđuje rezistenciju na holat koji ulazi u sastav žuči, što je značajno za preživljavanje laktokoka koje se primenjuju kao probiotici u digestivnom traktu ljudi i životinja. LmrP je sekundarni transporter i obezbeđuje rezistenciju na neke linkozamide, makrolide, streptogramine I tetracikline. CmbT je poslednji okarakterisan MDR transporter kod L. lactis i doprinosi rezistenciji na linkomicin, sulfonamidne antibiotike, sulfametoksazol i sulfadiazin, aminoglikozidni antibiotik streptomicin, aminonukleozidni antibiotik puromicin i semisintetski antibiotik rifampicin. Kako lanac hrane predstavlja važan put prenosa gena za rezistenciju u okviru ljudske i životinjske populacije, neophodno je intenzivno izučavanje mehanizama rezistencije kod laktokoka i drugih BMK namenjenih za upotrebu u prehrambenoj industriji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Resistance to antibiotics in lacid acid bacteria: Strain Lactococcus, Rezistencija na antibiotike kod bakterija mlečne kiseline - rod Lactococcus",
volume = "69",
number = "3-4",
pages = "271-281",
doi = "10.2298/VETGL1504271F"
}

Filipić, B., Golić, N., Jovčić, B., Ćupić-Miladinović, D., Soković, S., Popović, D.,& Kojić, M.. (2015). Resistance to antibiotics in lacid acid bacteria: Strain Lactococcus. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(3-4), 271-281.
https://doi.org/10.2298/VETGL1504271F

Filipić B, Golić N, Jovčić B, Ćupić-Miladinović D, Soković S, Popović D, Kojić M. Resistance to antibiotics in lacid acid bacteria: Strain Lactococcus. in Veterinarski glasnik. 2015;69(3-4):271-281.
doi:10.2298/VETGL1504271F .

Filipić, Brankica, Golić, Nataša, Jovčić, Branko, Ćupić-Miladinović, Dejana, Soković, Svetlana, Popović, Dušanka, Kojić, Milan, "Resistance to antibiotics in lacid acid bacteria: Strain Lactococcus" in Veterinarski glasnik, 69, no. 3-4 (2015):271-281,
https://doi.org/10.2298/VETGL1504271F . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Nikolić, Katarina
AU  - Filipić, Slavica
AU  - Jovčić, Branko
AU  - Agbaba, Danica
AU  - Antić-Stanković, Jelena
AU  - Kojić, Milan
AU  - Golić, Nataša
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2232
AB  - The CmbT substrate specificity and its role as a proton motive force-driven drug efflux pump at a molecular level were investigated in the study. In that order, 3D-quantitative structure-activity relationship (3D-QSAR) study was applied for selection of molecular determinants of multidrug recognition by CmbT. CmbT multidrug resistance protein of Lactococcus lactis contributes to extruding the structurally, chemically, and pharmacologically diverse range of substrates out of bacterial cells. This function of CmbT may result in the failure of antibiotic therapy. Homology model of CmbT protein was constructed and further opthnized. The 3D-QSAR model predictive potential was proved by use of leave-one-out cross validation Of the training set (Q(2): 0.69, R-observd(2) (vs).(Predicted) : 0.918, RMSEE: 0.193) and verification set (R-Observed vs predicted(2) : 0.704, RMSEP: 0.289). The results obtained in this study showed that high CmbT affinities to ethidium, sulbactam, and sulfathiazole could be related to the absence of significant unfavourable interactions. In contrast, the presence of specific unfavourable interaction between two hydrogen bond donor groups in bacitracin, apramycin, novobiocin, vancomycin, kanamycin, gentamycin, and tobramycin is found to be the main reason for their lower CmbT affinities. In addition, membrane position of the CmbT binding site and positive correlation between substrates lipophilicity (log D-PH so) and CmbT affinity strongly indicates that CmbT recognizes its substrates within the membrane.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of the Taiwan Institute of Chemical Engineers
T1  - Identifying the CmbT substrates specificity by using a quantitative structure-activity relationship (QSAR) study
VL  - 45
IS  - 3
SP  - 764
EP  - 771
DO  - 10.1016/j.jtice.2013.09.033
ER  - 

@article{
author = "Filipić, Brankica and Nikolić, Katarina and Filipić, Slavica and Jovčić, Branko and Agbaba, Danica and Antić-Stanković, Jelena and Kojić, Milan and Golić, Nataša",
year = "2014",
abstract = "The CmbT substrate specificity and its role as a proton motive force-driven drug efflux pump at a molecular level were investigated in the study. In that order, 3D-quantitative structure-activity relationship (3D-QSAR) study was applied for selection of molecular determinants of multidrug recognition by CmbT. CmbT multidrug resistance protein of Lactococcus lactis contributes to extruding the structurally, chemically, and pharmacologically diverse range of substrates out of bacterial cells. This function of CmbT may result in the failure of antibiotic therapy. Homology model of CmbT protein was constructed and further opthnized. The 3D-QSAR model predictive potential was proved by use of leave-one-out cross validation Of the training set (Q(2): 0.69, R-observd(2) (vs).(Predicted) : 0.918, RMSEE: 0.193) and verification set (R-Observed vs predicted(2) : 0.704, RMSEP: 0.289). The results obtained in this study showed that high CmbT affinities to ethidium, sulbactam, and sulfathiazole could be related to the absence of significant unfavourable interactions. In contrast, the presence of specific unfavourable interaction between two hydrogen bond donor groups in bacitracin, apramycin, novobiocin, vancomycin, kanamycin, gentamycin, and tobramycin is found to be the main reason for their lower CmbT affinities. In addition, membrane position of the CmbT binding site and positive correlation between substrates lipophilicity (log D-PH so) and CmbT affinity strongly indicates that CmbT recognizes its substrates within the membrane.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of the Taiwan Institute of Chemical Engineers",
title = "Identifying the CmbT substrates specificity by using a quantitative structure-activity relationship (QSAR) study",
volume = "45",
number = "3",
pages = "764-771",
doi = "10.1016/j.jtice.2013.09.033"
}

Filipić, B., Nikolić, K., Filipić, S., Jovčić, B., Agbaba, D., Antić-Stanković, J., Kojić, M.,& Golić, N.. (2014). Identifying the CmbT substrates specificity by using a quantitative structure-activity relationship (QSAR) study. in Journal of the Taiwan Institute of Chemical Engineers
Elsevier Science BV, Amsterdam., 45(3), 764-771.
https://doi.org/10.1016/j.jtice.2013.09.033

Filipić B, Nikolić K, Filipić S, Jovčić B, Agbaba D, Antić-Stanković J, Kojić M, Golić N. Identifying the CmbT substrates specificity by using a quantitative structure-activity relationship (QSAR) study. in Journal of the Taiwan Institute of Chemical Engineers. 2014;45(3):764-771.
doi:10.1016/j.jtice.2013.09.033 .

Filipić, Brankica, Nikolić, Katarina, Filipić, Slavica, Jovčić, Branko, Agbaba, Danica, Antić-Stanković, Jelena, Kojić, Milan, Golić, Nataša, "Identifying the CmbT substrates specificity by using a quantitative structure-activity relationship (QSAR) study" in Journal of the Taiwan Institute of Chemical Engineers, 45, no. 3 (2014):764-771,
https://doi.org/10.1016/j.jtice.2013.09.033 . .

TY  - JOUR
AU  - Uroić, Ksenija
AU  - Nikolić, Milica
AU  - Kos, Blazenka
AU  - Lebos-Pavunc, Andreja
AU  - Beganović, Jasna
AU  - Lukić, Jovanka
AU  - Jovčić, Branko
AU  - Filipić, Brankica
AU  - Miljković, Marija
AU  - Golić, Nataša
AU  - Topisirović, Ljubiša
AU  - Cadez, Neza
AU  - Raspor, Peter
AU  - Susković, Jagoda
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2197
AB  - The aim of this study is to gain insight into the probiotic potential of autochthonous lactic acid bacteria (LAB) isolated from artisanal fresh soft and white pickled cheeses. Eleven out of 86 LAB isolates from traditionally produced artisanal fresh soft and white pickled cheeses which survived the most rigorous simulated gastrointestinal tract conditions and did not show resistance to antibiotics were subjected to further evaluation for functional probiotic properties. The ability of the examined strains to assimilate cholesterol in the presence of bile salts was strain dependent, with the highest percentage of cholesterol assimilated by strain Lactobacillus brevis BGGO7-28 possessing S-layer proteins on its cell surface. The growth of strains with mannitol or lactulose as the only carbon source was better than with fructooligosaccharides (FOS) and inulin as prebiotic substrates, which should be considered in the production of synbiotics. Moreover, the results demonstrated that the strains were highly adhesive to human enterocyte-like Caco-2 cells and to a lesser extent to HT29-MTX cells, with the exception of strain Lb. brevis BGGO7-28, which showed similar percentage of adhesion to both cell lines. This strain was the only one with the acidic cell surface, while other examined strains have the cell surfaces with electron donor and basic properties. In addition, all selected strains decreased the proliferation of gut-associated lymphoid tissue (GALT) cells, suggesting possible immunomodulatory potential of the isolates. Finally, the number of viable cells in dry active preparations after lyophilisation depended on the lyoprotectant used (inulin, FOS or skimmed milk), as well as on the strain subjected to lyophilisation. In conclusion, the results obtained in this study demonstrate that particular dairy LAB isolates exhibit strain-specific probiotic properties. Thus, they could be further examined as part of mixed autochthonous starter cultures for traditional cheese production under controlled conditions.
PB  - Faculty Food Technology Biotechnology, Zagreb
T2  - Food Technology and Biotechnology
T1  - Probiotic Properties of Lactic Acid Bacteria Isolated from Croatian Fresh Soft Cheese and Serbian White Pickled Cheese
VL  - 52
IS  - 2
SP  - 232
EP  - 241
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2197
ER  - 

@article{
author = "Uroić, Ksenija and Nikolić, Milica and Kos, Blazenka and Lebos-Pavunc, Andreja and Beganović, Jasna and Lukić, Jovanka and Jovčić, Branko and Filipić, Brankica and Miljković, Marija and Golić, Nataša and Topisirović, Ljubiša and Cadez, Neza and Raspor, Peter and Susković, Jagoda",
year = "2014",
abstract = "The aim of this study is to gain insight into the probiotic potential of autochthonous lactic acid bacteria (LAB) isolated from artisanal fresh soft and white pickled cheeses. Eleven out of 86 LAB isolates from traditionally produced artisanal fresh soft and white pickled cheeses which survived the most rigorous simulated gastrointestinal tract conditions and did not show resistance to antibiotics were subjected to further evaluation for functional probiotic properties. The ability of the examined strains to assimilate cholesterol in the presence of bile salts was strain dependent, with the highest percentage of cholesterol assimilated by strain Lactobacillus brevis BGGO7-28 possessing S-layer proteins on its cell surface. The growth of strains with mannitol or lactulose as the only carbon source was better than with fructooligosaccharides (FOS) and inulin as prebiotic substrates, which should be considered in the production of synbiotics. Moreover, the results demonstrated that the strains were highly adhesive to human enterocyte-like Caco-2 cells and to a lesser extent to HT29-MTX cells, with the exception of strain Lb. brevis BGGO7-28, which showed similar percentage of adhesion to both cell lines. This strain was the only one with the acidic cell surface, while other examined strains have the cell surfaces with electron donor and basic properties. In addition, all selected strains decreased the proliferation of gut-associated lymphoid tissue (GALT) cells, suggesting possible immunomodulatory potential of the isolates. Finally, the number of viable cells in dry active preparations after lyophilisation depended on the lyoprotectant used (inulin, FOS or skimmed milk), as well as on the strain subjected to lyophilisation. In conclusion, the results obtained in this study demonstrate that particular dairy LAB isolates exhibit strain-specific probiotic properties. Thus, they could be further examined as part of mixed autochthonous starter cultures for traditional cheese production under controlled conditions.",
publisher = "Faculty Food Technology Biotechnology, Zagreb",
journal = "Food Technology and Biotechnology",
title = "Probiotic Properties of Lactic Acid Bacteria Isolated from Croatian Fresh Soft Cheese and Serbian White Pickled Cheese",
volume = "52",
number = "2",
pages = "232-241",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2197"
}

Uroić, K., Nikolić, M., Kos, B., Lebos-Pavunc, A., Beganović, J., Lukić, J., Jovčić, B., Filipić, B., Miljković, M., Golić, N., Topisirović, L., Cadez, N., Raspor, P.,& Susković, J.. (2014). Probiotic Properties of Lactic Acid Bacteria Isolated from Croatian Fresh Soft Cheese and Serbian White Pickled Cheese. in Food Technology and Biotechnology
Faculty Food Technology Biotechnology, Zagreb., 52(2), 232-241.
https://hdl.handle.net/21.15107/rcub_farfar_2197

Uroić K, Nikolić M, Kos B, Lebos-Pavunc A, Beganović J, Lukić J, Jovčić B, Filipić B, Miljković M, Golić N, Topisirović L, Cadez N, Raspor P, Susković J. Probiotic Properties of Lactic Acid Bacteria Isolated from Croatian Fresh Soft Cheese and Serbian White Pickled Cheese. in Food Technology and Biotechnology. 2014;52(2):232-241.
https://hdl.handle.net/21.15107/rcub_farfar_2197 .

Uroić, Ksenija, Nikolić, Milica, Kos, Blazenka, Lebos-Pavunc, Andreja, Beganović, Jasna, Lukić, Jovanka, Jovčić, Branko, Filipić, Brankica, Miljković, Marija, Golić, Nataša, Topisirović, Ljubiša, Cadez, Neza, Raspor, Peter, Susković, Jagoda, "Probiotic Properties of Lactic Acid Bacteria Isolated from Croatian Fresh Soft Cheese and Serbian White Pickled Cheese" in Food Technology and Biotechnology, 52, no. 2 (2014):232-241,
https://hdl.handle.net/21.15107/rcub_farfar_2197 .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Jovčić, Branko
AU  - Uzelac, Gordana
AU  - Miljković, Marija
AU  - Antić-Stanković, Jelena
AU  - Topisirović, Ljubiša
AU  - Golić, Nataša
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2045
AB  - The influence of the over-expression of CmbT multidrug resistance transporter on the growth rate of Lactococcus lactis NZ9000 was studied. L. lactis is a lactic acid bacteria (LAB) widely used as a starter culture in dairy industry. Recently characterized CmbT MDR transporter in L. lactis confers resistance to a wide variety of toxic compounds as well as to some clinically relevant antibiotics. In this study, the cmbT gene was over-expressed in the strain L. lactis NZ9000 in the presence of nisin inducer. Over-expression of the cmbT gene in L. lactis NZ9000 was followed by RT-PCR. The obtained results showed that the cmbT gene was successfully over-expressed by addition of sub-inhibitory amounts of nisin. Growth curves of L. lactis NZ9000/pCT50 over-expressing the cmbT gene and L. lactis NZ9000 control strain were followed in the rich medium as well as in the chemically defined medium in the presence solely of methionine (0.084 mM) or mix of methionine and cysteine (8.4 mM and 8.2 mM, respectively). Resulting doubling times revealed that L. lactis NZ9000/pCT50 had higher growth rate comparing to the control strain. This could be a consequence of the CmbT efflux activity, which improves the fitness of the host bacterium through the elimination of toxic compounds from the cell.
AB  - U ovom radu je izučavan uticaj povećane ekspresije cmbT gena, odgovornog za sintezu CmbT MDR transportera, na rast Lactococcus lactis. L. lactis pripada grupi bakterija mlečne kiseline (BMK) i ima veliku primenu u prehrambenoj industriji kao starter kultura. CmbT transporter je nedavno okarakterisan MDR protein soja L. lactis, koji doprinosi rezistenciji na različite toksične agense kao i na neke klinički značajne antibiotike. U ovom radu je cmbT gen višestruko eksprimiran u soju L. lactis NZ9000 dodavanjem nizina kao inducera. Povećana ekspresija cmbT gena je praćena metodom reverzne transkripcije (RT-PCR). Pokazano je da se nakon dodatka subinhibitornih koncentracija nizina u medijum za rast povećava količina sintetisane informacione RNK specifične za cmbT gen. Rast soja L. lactis NZ9000/pCT50, u kome je višestruko eksprimiran cmbT gen i L. lactis NZ9000 kontrolnog soja praćen je u bogatom i hemijski definisanom medijumu u prisustvu samo metionina (0.084 mM) ili kombinacije metionina i cisteina (8.4 mM i 8.2 mM). Praćene su krive rasta oba soja, a nakon izračunavanja odgovarajućih vremena generacije, rezultati su pokazali da L. lactis NZ9000/pCT50, brže raste u odnosu na kontrolni soj. Uočena razlika je najverovatnije posledica aktivnosti CmbT transportera koji doprinosi izbacivanju toksičnih agenasa iz ćelije i na taj način poboljšava adaptivne sposobnosti bakterije koja ga eksprimira i daje joj selektivnu prednost.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika, Belgrade
T1  - Over-expressed CmbT multidrug resistance transporter improves the fitness of Lactococcus lactis
T1  - Uticaj povećane ekspresije CmbT MDR transportera na rast Lactococcus lactis
VL  - 45
IS  - 1
SP  - 197
EP  - 206
DO  - 10.2298/GENSR1301197F
ER  - 

@article{
author = "Filipić, Brankica and Jovčić, Branko and Uzelac, Gordana and Miljković, Marija and Antić-Stanković, Jelena and Topisirović, Ljubiša and Golić, Nataša",
year = "2013",
abstract = "The influence of the over-expression of CmbT multidrug resistance transporter on the growth rate of Lactococcus lactis NZ9000 was studied. L. lactis is a lactic acid bacteria (LAB) widely used as a starter culture in dairy industry. Recently characterized CmbT MDR transporter in L. lactis confers resistance to a wide variety of toxic compounds as well as to some clinically relevant antibiotics. In this study, the cmbT gene was over-expressed in the strain L. lactis NZ9000 in the presence of nisin inducer. Over-expression of the cmbT gene in L. lactis NZ9000 was followed by RT-PCR. The obtained results showed that the cmbT gene was successfully over-expressed by addition of sub-inhibitory amounts of nisin. Growth curves of L. lactis NZ9000/pCT50 over-expressing the cmbT gene and L. lactis NZ9000 control strain were followed in the rich medium as well as in the chemically defined medium in the presence solely of methionine (0.084 mM) or mix of methionine and cysteine (8.4 mM and 8.2 mM, respectively). Resulting doubling times revealed that L. lactis NZ9000/pCT50 had higher growth rate comparing to the control strain. This could be a consequence of the CmbT efflux activity, which improves the fitness of the host bacterium through the elimination of toxic compounds from the cell., U ovom radu je izučavan uticaj povećane ekspresije cmbT gena, odgovornog za sintezu CmbT MDR transportera, na rast Lactococcus lactis. L. lactis pripada grupi bakterija mlečne kiseline (BMK) i ima veliku primenu u prehrambenoj industriji kao starter kultura. CmbT transporter je nedavno okarakterisan MDR protein soja L. lactis, koji doprinosi rezistenciji na različite toksične agense kao i na neke klinički značajne antibiotike. U ovom radu je cmbT gen višestruko eksprimiran u soju L. lactis NZ9000 dodavanjem nizina kao inducera. Povećana ekspresija cmbT gena je praćena metodom reverzne transkripcije (RT-PCR). Pokazano je da se nakon dodatka subinhibitornih koncentracija nizina u medijum za rast povećava količina sintetisane informacione RNK specifične za cmbT gen. Rast soja L. lactis NZ9000/pCT50, u kome je višestruko eksprimiran cmbT gen i L. lactis NZ9000 kontrolnog soja praćen je u bogatom i hemijski definisanom medijumu u prisustvu samo metionina (0.084 mM) ili kombinacije metionina i cisteina (8.4 mM i 8.2 mM). Praćene su krive rasta oba soja, a nakon izračunavanja odgovarajućih vremena generacije, rezultati su pokazali da L. lactis NZ9000/pCT50, brže raste u odnosu na kontrolni soj. Uočena razlika je najverovatnije posledica aktivnosti CmbT transportera koji doprinosi izbacivanju toksičnih agenasa iz ćelije i na taj način poboljšava adaptivne sposobnosti bakterije koja ga eksprimira i daje joj selektivnu prednost.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika, Belgrade",
title = "Over-expressed CmbT multidrug resistance transporter improves the fitness of Lactococcus lactis, Uticaj povećane ekspresije CmbT MDR transportera na rast Lactococcus lactis",
volume = "45",
number = "1",
pages = "197-206",
doi = "10.2298/GENSR1301197F"
}

Filipić, B., Jovčić, B., Uzelac, G., Miljković, M., Antić-Stanković, J., Topisirović, L.,& Golić, N.. (2013). Over-expressed CmbT multidrug resistance transporter improves the fitness of Lactococcus lactis. in Genetika, Belgrade
Društvo genetičara Srbije, Beograd., 45(1), 197-206.
https://doi.org/10.2298/GENSR1301197F

Filipić B, Jovčić B, Uzelac G, Miljković M, Antić-Stanković J, Topisirović L, Golić N. Over-expressed CmbT multidrug resistance transporter improves the fitness of Lactococcus lactis. in Genetika, Belgrade. 2013;45(1):197-206.
doi:10.2298/GENSR1301197F .

Filipić, Brankica, Jovčić, Branko, Uzelac, Gordana, Miljković, Marija, Antić-Stanković, Jelena, Topisirović, Ljubiša, Golić, Nataša, "Over-expressed CmbT multidrug resistance transporter improves the fitness of Lactococcus lactis" in Genetika, Belgrade, 45, no. 1 (2013):197-206,
https://doi.org/10.2298/GENSR1301197F . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Golić, Nataša
AU  - Jovčić, Branko
AU  - Tolinacki, Maja
AU  - Bay, Denice C.
AU  - Turner, Raymond J.
AU  - Antić-Stanković, Jelena
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1944
AB  - Functional characterization of the multidrug resistance CmbT transporter was performed in Lactococcus lactis. The cmbT gene is predicted to encode an efflux protein homologous to the multidrug resistance major facilitator superfamily. The cmbT gene (1377 bp) was cloned and overexpressed in L. lactis NZ9000. Results from cell growth studies revealed that the CmbT protein has an effect on host cell resistance to lincomycin, cholate, sulbactam, ethidium bromide, Hoechst 33342, sulfadiazine, streptomycin, rifampicin, puromycin and sulfametoxazole. Moreover, in vivo transport assays showed that overexpressed CmbT-mediated extrusion of ethidium bromide and Hoechst 33342 was higher than in the control L. lactis NZ9000 strain. CmbT-mediated extrusion of Hoechst 33342 was inhibited by the ionophores nigericin and valinomycin known to dissipate proton motive force. This indicates that CmbT-mediated extrusion is based on a drug-proton antiport mechanism. Taking together results obtained in this study, it can be concluded that CmbT is a novel major facilitator multidrug resistance transporter candidate in L. lactis, with a possible signaling role in sulfur metabolism.
PB  - Elsevier Science BV, Amsterdam
T2  - Research in Social & Administrative Pharmacy
T1  - The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis
VL  - 164
IS  - 1
SP  - 46
EP  - 54
DO  - 10.1016/j.resmic.2012.09.003
ER  - 

@article{
author = "Filipić, Brankica and Golić, Nataša and Jovčić, Branko and Tolinacki, Maja and Bay, Denice C. and Turner, Raymond J. and Antić-Stanković, Jelena and Kojić, Milan and Topisirović, Ljubiša",
year = "2013",
abstract = "Functional characterization of the multidrug resistance CmbT transporter was performed in Lactococcus lactis. The cmbT gene is predicted to encode an efflux protein homologous to the multidrug resistance major facilitator superfamily. The cmbT gene (1377 bp) was cloned and overexpressed in L. lactis NZ9000. Results from cell growth studies revealed that the CmbT protein has an effect on host cell resistance to lincomycin, cholate, sulbactam, ethidium bromide, Hoechst 33342, sulfadiazine, streptomycin, rifampicin, puromycin and sulfametoxazole. Moreover, in vivo transport assays showed that overexpressed CmbT-mediated extrusion of ethidium bromide and Hoechst 33342 was higher than in the control L. lactis NZ9000 strain. CmbT-mediated extrusion of Hoechst 33342 was inhibited by the ionophores nigericin and valinomycin known to dissipate proton motive force. This indicates that CmbT-mediated extrusion is based on a drug-proton antiport mechanism. Taking together results obtained in this study, it can be concluded that CmbT is a novel major facilitator multidrug resistance transporter candidate in L. lactis, with a possible signaling role in sulfur metabolism.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Research in Social & Administrative Pharmacy",
title = "The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis",
volume = "164",
number = "1",
pages = "46-54",
doi = "10.1016/j.resmic.2012.09.003"
}

Filipić, B., Golić, N., Jovčić, B., Tolinacki, M., Bay, D. C., Turner, R. J., Antić-Stanković, J., Kojić, M.,& Topisirović, L.. (2013). The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis. in Research in Social & Administrative Pharmacy
Elsevier Science BV, Amsterdam., 164(1), 46-54.
https://doi.org/10.1016/j.resmic.2012.09.003

Filipić B, Golić N, Jovčić B, Tolinacki M, Bay DC, Turner RJ, Antić-Stanković J, Kojić M, Topisirović L. The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis. in Research in Social & Administrative Pharmacy. 2013;164(1):46-54.
doi:10.1016/j.resmic.2012.09.003 .

Filipić, Brankica, Golić, Nataša, Jovčić, Branko, Tolinacki, Maja, Bay, Denice C., Turner, Raymond J., Antić-Stanković, Jelena, Kojić, Milan, Topisirović, Ljubiša, "The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis" in Research in Social & Administrative Pharmacy, 164, no. 1 (2013):46-54,
https://doi.org/10.1016/j.resmic.2012.09.003 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Strahinić, Ivana
AU  - Milenković, Marina
AU  - Golić, Nataša
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
AU  - Begović, Jelena
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1953
AB  - The present study was carried out to test the colonic mucosal response of rats to oral supplementation with Lactobacillus fermentum BGHI14 and to correlate the tissue reaction to trinitrobenzenesulfonate (TNBS)-induced colitis with mucosal barrier alterations caused by bacterial ingestion. An immune cell-mediated reaction of healthy colonic tissue was noticed after bacterial feeding. After prolonged bacterial treatment, the observed reaction had retreated to normality, but the mRNA levels of proinflammatory cytokines interleukin-1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF-alpha) remained elevated. These data point to the chronic low-grade inflammation that could be caused by long-term probiotic consumption. Although no detrimental effects of bacterial pretreatment were noticed in colitic rats, at least in the acute state of disease, the results obtained in our study point to the necessity of reassessment of existing data on the safety of probiotic preparations. Additionally, probiotic effects in experimental colitis models might depend on time coordination of disease induction with treatment duration.
PB  - Amer Soc Microbiology, Washington
T2  - Applied and Environmental Microbiology
T1  - Interaction of Lactobacillus fermentum BGHI14 with Rat Colonic Mucosa: Implications for Colitis Induction
VL  - 79
IS  - 18
SP  - 5735
EP  - 5744
DO  - 10.1128/AEM.01807-13
ER  - 

@article{
author = "Lukić, Jovanka and Strahinić, Ivana and Milenković, Marina and Golić, Nataša and Kojić, Milan and Topisirović, Ljubiša and Begović, Jelena",
year = "2013",
abstract = "The present study was carried out to test the colonic mucosal response of rats to oral supplementation with Lactobacillus fermentum BGHI14 and to correlate the tissue reaction to trinitrobenzenesulfonate (TNBS)-induced colitis with mucosal barrier alterations caused by bacterial ingestion. An immune cell-mediated reaction of healthy colonic tissue was noticed after bacterial feeding. After prolonged bacterial treatment, the observed reaction had retreated to normality, but the mRNA levels of proinflammatory cytokines interleukin-1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF-alpha) remained elevated. These data point to the chronic low-grade inflammation that could be caused by long-term probiotic consumption. Although no detrimental effects of bacterial pretreatment were noticed in colitic rats, at least in the acute state of disease, the results obtained in our study point to the necessity of reassessment of existing data on the safety of probiotic preparations. Additionally, probiotic effects in experimental colitis models might depend on time coordination of disease induction with treatment duration.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Applied and Environmental Microbiology",
title = "Interaction of Lactobacillus fermentum BGHI14 with Rat Colonic Mucosa: Implications for Colitis Induction",
volume = "79",
number = "18",
pages = "5735-5744",
doi = "10.1128/AEM.01807-13"
}

Lukić, J., Strahinić, I., Milenković, M., Golić, N., Kojić, M., Topisirović, L.,& Begović, J.. (2013). Interaction of Lactobacillus fermentum BGHI14 with Rat Colonic Mucosa: Implications for Colitis Induction. in Applied and Environmental Microbiology
Amer Soc Microbiology, Washington., 79(18), 5735-5744.
https://doi.org/10.1128/AEM.01807-13

Lukić J, Strahinić I, Milenković M, Golić N, Kojić M, Topisirović L, Begović J. Interaction of Lactobacillus fermentum BGHI14 with Rat Colonic Mucosa: Implications for Colitis Induction. in Applied and Environmental Microbiology. 2013;79(18):5735-5744.
doi:10.1128/AEM.01807-13 .

Lukić, Jovanka, Strahinić, Ivana, Milenković, Marina, Golić, Nataša, Kojić, Milan, Topisirović, Ljubiša, Begović, Jelena, "Interaction of Lactobacillus fermentum BGHI14 with Rat Colonic Mucosa: Implications for Colitis Induction" in Applied and Environmental Microbiology, 79, no. 18 (2013):5735-5744,
https://doi.org/10.1128/AEM.01807-13 . .