TY  - JOUR
AU  - Boškov, Ivana A.
AU  - Savić, Ivan M.
AU  - Grozdanić Stanisavljević, Nađa Ð.
AU  - Kundaković-Vasović, Tatjana
AU  - Radović-Selgrad, Jelena
AU  - Savić Gajić, Ivana M.
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5573
AB  - Black locust flower extract contains various polyphenols and their glucosides contribute to the potential health benefits. After intake of these bioactive compounds and passage through the gastrointestinal tract, their degradation can occur and lead to a loss of biological activity. To overcome this problem, the bioactive compounds should be protected from environmental conditions. This study aimed to encapsulate the black flower extract in the microparticles based on biodegradable polysaccharides, alginate, and chitosan. In the extract, the total antioxidant content was found to be 3.18 ± 0.01 g gallic acid equivalent per 100 g of dry weight. Also, the presence of lipids (16), phenolics (27), organic acids (4), L-aspartic acid derivative, questinol, gibberellic acid, sterol, and saponins (2) was confirmed using the UHPLC–ESI–MS analysis. In vitro assays showed that the extract has weak anti-α-glucosidase activity and moderate antioxidant and cytotoxic activity against the HeLa cell line. The extrusion method with secondary air flow enabled the preparation of microparticles (about 270 μm) encapsulated with extract. An encapsulation efficiency of over 92% was achieved in the alginate and alginate–chitosan microparticles. The swelling study confirmed a lower permeability of alginate–chitosan microparticles compared with alginate microparticles. For both types of microparticles, the release profile of antioxidants in the simulated gastrointestinal fluids at 37 °C followed the Korsmeyer–Peppas model. A lower diffusion coefficient than 0.5 indicated the simple Fick diffusion of antioxidants. The alginate–chitosan microparticles enabled a more sustained release of antioxidants from extract compared to the alginate microparticles. The obtained results indicated an improvement in the antioxidant activity of bioactive compounds from the extract and their protection from degradation in the simulated gastric conditions via encapsulation in the polymer matrixes. Alginate–chitosan showed slightly slower cumulative antioxidant release from microparticles and better antioxidant activity of the extract compared to the alginate system. According to these results, alginate–chitosan microparticles are more suitable for further application in the encapsulation of black locust flower extract. Also, the proposed polymer matrix as a drug delivery system is safe for human use due to its biodegradability and non-toxicity.
PB  - MDPI
T2  - Polymers
T1  - Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles
VL  - 16
IS  - 5
DO  - 10.3390/polym16050688
ER  - 

@article{
author = "Boškov, Ivana A. and Savić, Ivan M. and Grozdanić Stanisavljević, Nađa Ð. and Kundaković-Vasović, Tatjana and Radović-Selgrad, Jelena and Savić Gajić, Ivana M.",
year = "2024",
abstract = "Black locust flower extract contains various polyphenols and their glucosides contribute to the potential health benefits. After intake of these bioactive compounds and passage through the gastrointestinal tract, their degradation can occur and lead to a loss of biological activity. To overcome this problem, the bioactive compounds should be protected from environmental conditions. This study aimed to encapsulate the black flower extract in the microparticles based on biodegradable polysaccharides, alginate, and chitosan. In the extract, the total antioxidant content was found to be 3.18 ± 0.01 g gallic acid equivalent per 100 g of dry weight. Also, the presence of lipids (16), phenolics (27), organic acids (4), L-aspartic acid derivative, questinol, gibberellic acid, sterol, and saponins (2) was confirmed using the UHPLC–ESI–MS analysis. In vitro assays showed that the extract has weak anti-α-glucosidase activity and moderate antioxidant and cytotoxic activity against the HeLa cell line. The extrusion method with secondary air flow enabled the preparation of microparticles (about 270 μm) encapsulated with extract. An encapsulation efficiency of over 92% was achieved in the alginate and alginate–chitosan microparticles. The swelling study confirmed a lower permeability of alginate–chitosan microparticles compared with alginate microparticles. For both types of microparticles, the release profile of antioxidants in the simulated gastrointestinal fluids at 37 °C followed the Korsmeyer–Peppas model. A lower diffusion coefficient than 0.5 indicated the simple Fick diffusion of antioxidants. The alginate–chitosan microparticles enabled a more sustained release of antioxidants from extract compared to the alginate microparticles. The obtained results indicated an improvement in the antioxidant activity of bioactive compounds from the extract and their protection from degradation in the simulated gastric conditions via encapsulation in the polymer matrixes. Alginate–chitosan showed slightly slower cumulative antioxidant release from microparticles and better antioxidant activity of the extract compared to the alginate system. According to these results, alginate–chitosan microparticles are more suitable for further application in the encapsulation of black locust flower extract. Also, the proposed polymer matrix as a drug delivery system is safe for human use due to its biodegradability and non-toxicity.",
publisher = "MDPI",
journal = "Polymers",
title = "Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles",
volume = "16",
number = "5",
doi = "10.3390/polym16050688"
}

Boškov, I. A., Savić, I. M., Grozdanić Stanisavljević, N. Ð., Kundaković-Vasović, T., Radović-Selgrad, J.,& Savić Gajić, I. M.. (2024). Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles. in Polymers
MDPI., 16(5).
https://doi.org/10.3390/polym16050688

Boškov IA, Savić IM, Grozdanić Stanisavljević NÐ, Kundaković-Vasović T, Radović-Selgrad J, Savić Gajić IM. Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles. in Polymers. 2024;16(5).
doi:10.3390/polym16050688 .

Boškov, Ivana A., Savić, Ivan M., Grozdanić Stanisavljević, Nađa Ð., Kundaković-Vasović, Tatjana, Radović-Selgrad, Jelena, Savić Gajić, Ivana M., "Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles" in Polymers, 16, no. 5 (2024),
https://doi.org/10.3390/polym16050688 . .

TY  - CONF
AU  - Radović-Selgrad, Jelena
AU  - Milutinović, Violeta
AU  - Dragićević-Sudimac, Katarina
AU  - Vićentijević, Lazar
AU  - Kopanja, Đurđica
AU  - Kundaković-Vasović, Tatjana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5149
AB  - Sorbus torminalis (L.) Crantz (Rosaceae), брекиња, је листопадно дрво средње величине које потиче из централне и јужне Европе, северозападне Африке, Балканског полуострва и Мале Азије. Разни делови биљке, укључујући плод, лист, цваст и кору брекиње, традиционално се користе као диуретици, антид ијароици, антиинфламатори, антидијабетици и антиатерогена средства, вазопротективи и вазорелаксанси, али и као добри антиоксиданси. ...
AB  - Sorbus torminalis (L.) Crantz (Rosaceae) is a medium-sized deciduous tree native to central and southern Europe, northwestern Africa, the Balkan Peninsula, and western Asia. Various parts of the plant, including the fruit, leaf, inflorescence, and bark are traditionally used as diuretics, antidiarrheals, anti-inflammatory, antidiabetic and antiatherogenic agents, vasoprotective and vasorelaxants, but also as potent antioxidants. ...
PB  - Ботаничко друштво „Андреас Волни”, Нови Сад
PB  - Матица српска, Одељење за природне науке, Нови Сад
C3  - 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
T1  - Ензимска инхибиторна својства метанолног екстракта коре стабла брекиње, Sorbus torminalis (L.) Crantz
T1  - Enzyme inhibitory properties of the methanol extract of the tree bark of Sorbus torminalis (L.) Crantz
SP  - 39
EP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5149
ER  - 

@conference{
author = "Radović-Selgrad, Jelena and Milutinović, Violeta and Dragićević-Sudimac, Katarina and Vićentijević, Lazar and Kopanja, Đurđica and Kundaković-Vasović, Tatjana",
year = "2023",
abstract = "Sorbus torminalis (L.) Crantz (Rosaceae), брекиња, је листопадно дрво средње величине које потиче из централне и јужне Европе, северозападне Африке, Балканског полуострва и Мале Азије. Разни делови биљке, укључујући плод, лист, цваст и кору брекиње, традиционално се користе као диуретици, антид ијароици, антиинфламатори, антидијабетици и антиатерогена средства, вазопротективи и вазорелаксанси, али и као добри антиоксиданси. ..., Sorbus torminalis (L.) Crantz (Rosaceae) is a medium-sized deciduous tree native to central and southern Europe, northwestern Africa, the Balkan Peninsula, and western Asia. Various parts of the plant, including the fruit, leaf, inflorescence, and bark are traditionally used as diuretics, antidiarrheals, anti-inflammatory, antidiabetic and antiatherogenic agents, vasoprotective and vasorelaxants, but also as potent antioxidants. ...",
publisher = "Ботаничко друштво „Андреас Волни”, Нови Сад, Матица српска, Одељење за природне науке, Нови Сад",
journal = "2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака",
title = "Ензимска инхибиторна својства метанолног екстракта коре стабла брекиње, Sorbus torminalis (L.) Crantz, Enzyme inhibitory properties of the methanol extract of the tree bark of Sorbus torminalis (L.) Crantz",
pages = "39-41",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5149"
}

Radović-Selgrad, J., Milutinović, V., Dragićević-Sudimac, K., Vićentijević, L., Kopanja, Đ.,& Kundaković-Vasović, T.. (2023). Ензимска инхибиторна својства метанолног екстракта коре стабла брекиње, Sorbus torminalis (L.) Crantz. in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
Ботаничко друштво „Андреас Волни”, Нови Сад., 39-41.
https://hdl.handle.net/21.15107/rcub_farfar_5149

Radović-Selgrad J, Milutinović V, Dragićević-Sudimac K, Vićentijević L, Kopanja Đ, Kundaković-Vasović T. Ензимска инхибиторна својства метанолног екстракта коре стабла брекиње, Sorbus torminalis (L.) Crantz. in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака. 2023;:39-41.
https://hdl.handle.net/21.15107/rcub_farfar_5149 .

Radović-Selgrad, Jelena, Milutinović, Violeta, Dragićević-Sudimac, Katarina, Vićentijević, Lazar, Kopanja, Đurđica, Kundaković-Vasović, Tatjana, "Ензимска инхибиторна својства метанолног екстракта коре стабла брекиње, Sorbus torminalis (L.) Crantz" in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака (2023):39-41,
https://hdl.handle.net/21.15107/rcub_farfar_5149 .

TY  - CONF
AU  - Leković, Aleksandra
AU  - Samardžić, Stevan
AU  - Radović-Selgrad, Jelena
AU  - Kundaković-Vasović, Tatjana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4584
AB  - Sorbus torminalis (L.) Crantz (Rosaceae), also known as wild service tree, is a
deciduous tree widespread in central and southern Europe, north-western Africa, western
Asia, the Balkan Peninsula and the Caucasus (1, 2). Species of the genus Sorbus represent a
rich source of polyphenols that are responsible for their numerous proven effects, including
anti-diarrhoeal, anti-inflammatory, antidiabetic, antioxidant and anti-atherogenic effect.
Accordingly, the bark of S. torminalis could have interesting composition and effects,
however, data about this herbal drug is insufficient (1). Bark dry methanol extract (DER
9.74:1), obtained after successive extraction with cyclohexane and dichloromethane, was
analyzed for antioxidant potential using DPPH and FRAP assay. Contents of total
polyphenols, flavonoids and tannins were determined using spectrophotometric methods
based on reaction with Folin-Ciocalteu reagent (FC), reaction with aluminum-chloride and
adsorption by hide powder in combination with reaction with FC, respectively. LC-DAD-ESI-
MS analysis was used for identification of main extract constituents. In DPPH test IC50 value
8.66±0.07 μg/mL was shown, while result of FRAP test was 3.01±0.11 μmol Fe 2+ /mg of dry
extract. Contents of total polyphenols, flavonoids and tannins were 0.39±0.06 mg GA/mg of
dry extract, 0.035±0.019% and 1.3±0.08%, respectively. By LC-DAD-ESI-MS analysis 25
compounds were detected among which procyanidins (dimers, trimers and tetramers) are
dominant. The results of this study showed that dry methanol extract of S. torminalis bark is
rich in procyanidins which potentially contribute to its antioxidant activity. Additional
significant effects of this herbal drug and its isolated compounds should be investigated in
future studies.
AB  - Sorbus torminalis (L.) Crantz (Rosaceae) je listopadno drvo, u narodu poznato pod
nazivom brekinja. Rasprostranjeno je širom centralne i južne Evrope, severozapadne Afrike,
zapadne Azije, Balkanskog poluostrva i Kavkaza (1, 2). Vrste roda Sorbus predstavljaju bogat
izvor polifenola koji su odgovorni za njihove brojne dokazane efekte, uključuju antidijaroični,
antiinflamatorni, antidijabetski, antioksidantni i antiaterogeni efekat. U skladu sa tim, kora S.
torminalis mogla bi imati interesantan sastav i efekte, međutim, o ovoj biljnoj drogi nema
dovoljno podataka (1). Antioksidantna aktivnost suvog metanolnog ekstrakta kore stabla
(DER 9,74:1), dobijenog nakon sukcesivne ekstrakcije cikloheksanom i dihlormetanom,
analizirana je DPPH i FRAP testom. Sadržaji ukupnih polifenola, flavonoida i tanina određeni
su spektrofotometrijskim metodama koje se zasnivaju na reakciji sa Folin‐Ciocalteu
reagensom (FC), reakciji sa aluminijum-hloridom i na adsorpciji na kožni prah u kombinaciji
sa reakcijom sa FC reagensom, redom. LC‐DAD‐ESI‐MS analiza korišćena je za identifikaciju
glavnih sastojaka ekstrakta. U DPPH testu dobijena je IC50 vrednost 8,66±0,07 μg/mL, a
rezultat FRAP testa bio je 3,01±0,11 μmol Fe 2+ /mg suvog ekstrakta. Sadžaj ukupnih
polifenola bio je 0,39±0,06 mg GA/mg suvog ekstrakta, flavonoida 0,035±0,019%, a tanina
1,3±0,08%. LC‐DAD‐ESI‐MS analizom detektovano je 25 jedinjenja od kojih su dominantni
procijanidini (dimeri, trimeri i tetrameri). Rezultati ovog istraživanja pokazali su da je suvi
metanolni ekstrakt kore stabla S. torminalis bogat procijanidinima koji potencijalno
doprinose njegovoj antioksidantnoj aktivnosti. Dodatne značajne efekte ove biljne droge i
njenih izolovanih jedinjenja trebalo bi ispitati u budućim istraživanjima.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Chemical composition and antioxidant activity of Sorbus torminalis bark methanol extract
T1  - Hemijski sastav i antioksidantna aktivnost metanolnog ekstrakta kore stabla Sorbus torminalis
VL  - 72
IS  - 4 suplement
SP  - S467
EP  - S468
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4584
ER  - 

@conference{
author = "Leković, Aleksandra and Samardžić, Stevan and Radović-Selgrad, Jelena and Kundaković-Vasović, Tatjana",
year = "2022",
abstract = "Sorbus torminalis (L.) Crantz (Rosaceae), also known as wild service tree, is a
deciduous tree widespread in central and southern Europe, north-western Africa, western
Asia, the Balkan Peninsula and the Caucasus (1, 2). Species of the genus Sorbus represent a
rich source of polyphenols that are responsible for their numerous proven effects, including
anti-diarrhoeal, anti-inflammatory, antidiabetic, antioxidant and anti-atherogenic effect.
Accordingly, the bark of S. torminalis could have interesting composition and effects,
however, data about this herbal drug is insufficient (1). Bark dry methanol extract (DER
9.74:1), obtained after successive extraction with cyclohexane and dichloromethane, was
analyzed for antioxidant potential using DPPH and FRAP assay. Contents of total
polyphenols, flavonoids and tannins were determined using spectrophotometric methods
based on reaction with Folin-Ciocalteu reagent (FC), reaction with aluminum-chloride and
adsorption by hide powder in combination with reaction with FC, respectively. LC-DAD-ESI-
MS analysis was used for identification of main extract constituents. In DPPH test IC50 value
8.66±0.07 μg/mL was shown, while result of FRAP test was 3.01±0.11 μmol Fe 2+ /mg of dry
extract. Contents of total polyphenols, flavonoids and tannins were 0.39±0.06 mg GA/mg of
dry extract, 0.035±0.019% and 1.3±0.08%, respectively. By LC-DAD-ESI-MS analysis 25
compounds were detected among which procyanidins (dimers, trimers and tetramers) are
dominant. The results of this study showed that dry methanol extract of S. torminalis bark is
rich in procyanidins which potentially contribute to its antioxidant activity. Additional
significant effects of this herbal drug and its isolated compounds should be investigated in
future studies., Sorbus torminalis (L.) Crantz (Rosaceae) je listopadno drvo, u narodu poznato pod
nazivom brekinja. Rasprostranjeno je širom centralne i južne Evrope, severozapadne Afrike,
zapadne Azije, Balkanskog poluostrva i Kavkaza (1, 2). Vrste roda Sorbus predstavljaju bogat
izvor polifenola koji su odgovorni za njihove brojne dokazane efekte, uključuju antidijaroični,
antiinflamatorni, antidijabetski, antioksidantni i antiaterogeni efekat. U skladu sa tim, kora S.
torminalis mogla bi imati interesantan sastav i efekte, međutim, o ovoj biljnoj drogi nema
dovoljno podataka (1). Antioksidantna aktivnost suvog metanolnog ekstrakta kore stabla
(DER 9,74:1), dobijenog nakon sukcesivne ekstrakcije cikloheksanom i dihlormetanom,
analizirana je DPPH i FRAP testom. Sadržaji ukupnih polifenola, flavonoida i tanina određeni
su spektrofotometrijskim metodama koje se zasnivaju na reakciji sa Folin‐Ciocalteu
reagensom (FC), reakciji sa aluminijum-hloridom i na adsorpciji na kožni prah u kombinaciji
sa reakcijom sa FC reagensom, redom. LC‐DAD‐ESI‐MS analiza korišćena je za identifikaciju
glavnih sastojaka ekstrakta. U DPPH testu dobijena je IC50 vrednost 8,66±0,07 μg/mL, a
rezultat FRAP testa bio je 3,01±0,11 μmol Fe 2+ /mg suvog ekstrakta. Sadžaj ukupnih
polifenola bio je 0,39±0,06 mg GA/mg suvog ekstrakta, flavonoida 0,035±0,019%, a tanina
1,3±0,08%. LC‐DAD‐ESI‐MS analizom detektovano je 25 jedinjenja od kojih su dominantni
procijanidini (dimeri, trimeri i tetrameri). Rezultati ovog istraživanja pokazali su da je suvi
metanolni ekstrakt kore stabla S. torminalis bogat procijanidinima koji potencijalno
doprinose njegovoj antioksidantnoj aktivnosti. Dodatne značajne efekte ove biljne droge i
njenih izolovanih jedinjenja trebalo bi ispitati u budućim istraživanjima.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Chemical composition and antioxidant activity of Sorbus torminalis bark methanol extract, Hemijski sastav i antioksidantna aktivnost metanolnog ekstrakta kore stabla Sorbus torminalis",
volume = "72",
number = "4 suplement",
pages = "S467-S468",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4584"
}

Leković, A., Samardžić, S., Radović-Selgrad, J.,& Kundaković-Vasović, T.. (2022). Chemical composition and antioxidant activity of Sorbus torminalis bark methanol extract. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S467-S468.
https://hdl.handle.net/21.15107/rcub_farfar_4584

Leković A, Samardžić S, Radović-Selgrad J, Kundaković-Vasović T. Chemical composition and antioxidant activity of Sorbus torminalis bark methanol extract. in Arhiv za farmaciju. 2022;72(4 suplement):S467-S468.
https://hdl.handle.net/21.15107/rcub_farfar_4584 .

Leković, Aleksandra, Samardžić, Stevan, Radović-Selgrad, Jelena, Kundaković-Vasović, Tatjana, "Chemical composition and antioxidant activity of Sorbus torminalis bark methanol extract" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S467-S468,
https://hdl.handle.net/21.15107/rcub_farfar_4584 .

TY  - CONF
AU  - Radović-Selgrad, Jelena
AU  - Ušjak, Dušan
AU  - Milenković, Marina
AU  - Kundaković-Vasović, Tatjana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4580
AB  - Dry methanol extract (DER 3.95:1) of aerial flowering parts of Alchemilla viridiflora
Rothm., Rosaceae, obtained after successive extraction with cyclohexane and
dichloromethane, predominantly contains polyphenols, tannins (3.74%), mostly ellagic, and
flavonoids (0.30%), hexosides of quercetin and kaempferol. It shows ACE inhibitory and
anti-Helicobacter pylori activity in vitro (1). Based on the traditional use of the common A.
vulgaris in the treatment of diabetes (2), the effect of this A. viridiflora extract on α-amylase
activity in vitro and lowering blood glucose in rats were investigated. α-Amylase inhibitory
activity was assessed using the 3,5-dinitrosalicylic acid method. Extract in doses of 50, 100
and 200 mg/kg, p.o., was administered to streptozotocin-induced diabetic rats for 20 days,
and blood glucose level and weight of rats were monitored during treatment. Extract
inhibited α-amylase activity at IC 50 5.47 ± 0.30 mg/mL. A dose of 200 mg/kg significantly
decreased blood glucose level after 10 (32.2%) and 20 days of treatment (38.3%). The effect
of this dose was similar to the effect of the reference drug, glibenclamide. A dose of 100
mg/kg significantly increased glucose level by day 10 (50.5%) and a reduction in blood sugar
level of 51.2% was observed only after 10 days. Body weight was correlated with changes in
blood sugar levels. A dose of 50 mg/kg had no statistically significant effect. Results indicate
a significant potential of A. viridiflora aerial parts in the treatment of diabetes, which must be
confirmed by additional studies, and active ingredients need to be identified.
AB  - Suvi metanolni ekstrakt (DER 3,95:1) nadzemnih delova u cvetu Alchemilla viridiflora
Rothm., Rosaceae, dobijen nakon sukcesivne ekstrakcije cikloheksanom i dihlormetanom,
sadrži visok nivo polifenola, tanina (3,74%), uglavnom elagnih, i flavonoida (0,30%),
heksozida kvercetina i kemferola. Pokazuje ACE inhibitornu i anti-Helicobacter pylori
aktivnost in vitro (1). Na osnovu podataka o tradicionalnoj upotrebi herbe A. vulgaris,
najčešće korišćene Alchemilla vrste, u lečenju dijabetesa (2), ispitivan je uticaj navedenog
ekstrakta A. viridiflora na aktivnost α-amilaze in vitro i na snižavanje nivoa glukoze u krvi
kod pacova. Uticaj na aktivnost α-amilaze procenjen je primenom metode sa 3,5-
dinitrosalicilnom kiselinom. Ekstrakt A. viridiflora u dozama od 50, 100 i 200 mg/kg, p.o.,
aplikovan je pacovima sa dijabetesom izazvanim streptozotocinom tokom 20 dana, a nivoi
glukoze u krvi i težina pacova su praćeni u određenim vremenskim intervalima tokom
trajanja tretmana. Ekstrakt je inhibirao aktivnost α-amilaze, a vrednost IC 50 iznosila je
5,47±0,30 mg/mL. Doza od 200 mg/kg značajno je smanjila nivo glukoze u krvi nakon 10
(32,2%) i 20 dana tretmana (38,3%). Efekat ove doze bio je sličan efektu referentnog leka,
glibenklamida. S druge strane, doza od 100 mg/kg značajno je povećala nivo glukoze do 10.
dana (50,5%) a do značajnog poboljšanja, tj. sniženja nivoa šećera od 51,2% dovela je tek
nakon 10 dana. Telesna težina bila je u korelaciji sa promenama nivoa šećera u krvi. Doza od
50 mg/kg nije imala statistički značajan efekat. Rezultati ukazuju na značajan potencijal
herbe A. viridiflora u lečenju dijabetesa, što mora biti potvrđeno dodatnim studijama, a
aktivni sastojci identifikovani.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Inhibition of A-amylase in vitro and in vivo hypoglycemic effect of methanol extract of Alchemilla viridiflora Rothm
T1  - Inhibicija Α‐amilaze in vitro i in vivo hipoglikemijski efekat metanolnog ekstrakta herbe Alchemilla viridiflora Rothm
VL  - 72
IS  - 4 suplement
SP  - S461
EP  - S462
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4580
ER  - 

@conference{
author = "Radović-Selgrad, Jelena and Ušjak, Dušan and Milenković, Marina and Kundaković-Vasović, Tatjana",
year = "2022",
abstract = "Dry methanol extract (DER 3.95:1) of aerial flowering parts of Alchemilla viridiflora
Rothm., Rosaceae, obtained after successive extraction with cyclohexane and
dichloromethane, predominantly contains polyphenols, tannins (3.74%), mostly ellagic, and
flavonoids (0.30%), hexosides of quercetin and kaempferol. It shows ACE inhibitory and
anti-Helicobacter pylori activity in vitro (1). Based on the traditional use of the common A.
vulgaris in the treatment of diabetes (2), the effect of this A. viridiflora extract on α-amylase
activity in vitro and lowering blood glucose in rats were investigated. α-Amylase inhibitory
activity was assessed using the 3,5-dinitrosalicylic acid method. Extract in doses of 50, 100
and 200 mg/kg, p.o., was administered to streptozotocin-induced diabetic rats for 20 days,
and blood glucose level and weight of rats were monitored during treatment. Extract
inhibited α-amylase activity at IC 50 5.47 ± 0.30 mg/mL. A dose of 200 mg/kg significantly
decreased blood glucose level after 10 (32.2%) and 20 days of treatment (38.3%). The effect
of this dose was similar to the effect of the reference drug, glibenclamide. A dose of 100
mg/kg significantly increased glucose level by day 10 (50.5%) and a reduction in blood sugar
level of 51.2% was observed only after 10 days. Body weight was correlated with changes in
blood sugar levels. A dose of 50 mg/kg had no statistically significant effect. Results indicate
a significant potential of A. viridiflora aerial parts in the treatment of diabetes, which must be
confirmed by additional studies, and active ingredients need to be identified., Suvi metanolni ekstrakt (DER 3,95:1) nadzemnih delova u cvetu Alchemilla viridiflora
Rothm., Rosaceae, dobijen nakon sukcesivne ekstrakcije cikloheksanom i dihlormetanom,
sadrži visok nivo polifenola, tanina (3,74%), uglavnom elagnih, i flavonoida (0,30%),
heksozida kvercetina i kemferola. Pokazuje ACE inhibitornu i anti-Helicobacter pylori
aktivnost in vitro (1). Na osnovu podataka o tradicionalnoj upotrebi herbe A. vulgaris,
najčešće korišćene Alchemilla vrste, u lečenju dijabetesa (2), ispitivan je uticaj navedenog
ekstrakta A. viridiflora na aktivnost α-amilaze in vitro i na snižavanje nivoa glukoze u krvi
kod pacova. Uticaj na aktivnost α-amilaze procenjen je primenom metode sa 3,5-
dinitrosalicilnom kiselinom. Ekstrakt A. viridiflora u dozama od 50, 100 i 200 mg/kg, p.o.,
aplikovan je pacovima sa dijabetesom izazvanim streptozotocinom tokom 20 dana, a nivoi
glukoze u krvi i težina pacova su praćeni u određenim vremenskim intervalima tokom
trajanja tretmana. Ekstrakt je inhibirao aktivnost α-amilaze, a vrednost IC 50 iznosila je
5,47±0,30 mg/mL. Doza od 200 mg/kg značajno je smanjila nivo glukoze u krvi nakon 10
(32,2%) i 20 dana tretmana (38,3%). Efekat ove doze bio je sličan efektu referentnog leka,
glibenklamida. S druge strane, doza od 100 mg/kg značajno je povećala nivo glukoze do 10.
dana (50,5%) a do značajnog poboljšanja, tj. sniženja nivoa šećera od 51,2% dovela je tek
nakon 10 dana. Telesna težina bila je u korelaciji sa promenama nivoa šećera u krvi. Doza od
50 mg/kg nije imala statistički značajan efekat. Rezultati ukazuju na značajan potencijal
herbe A. viridiflora u lečenju dijabetesa, što mora biti potvrđeno dodatnim studijama, a
aktivni sastojci identifikovani.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Inhibition of A-amylase in vitro and in vivo hypoglycemic effect of methanol extract of Alchemilla viridiflora Rothm, Inhibicija Α‐amilaze in vitro i in vivo hipoglikemijski efekat metanolnog ekstrakta herbe Alchemilla viridiflora Rothm",
volume = "72",
number = "4 suplement",
pages = "S461-S462",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4580"
}

Radović-Selgrad, J., Ušjak, D., Milenković, M.,& Kundaković-Vasović, T.. (2022). Inhibition of A-amylase in vitro and in vivo hypoglycemic effect of methanol extract of Alchemilla viridiflora Rothm. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S461-S462.
https://hdl.handle.net/21.15107/rcub_farfar_4580

Radović-Selgrad J, Ušjak D, Milenković M, Kundaković-Vasović T. Inhibition of A-amylase in vitro and in vivo hypoglycemic effect of methanol extract of Alchemilla viridiflora Rothm. in Arhiv za farmaciju. 2022;72(4 suplement):S461-S462.
https://hdl.handle.net/21.15107/rcub_farfar_4580 .

Radović-Selgrad, Jelena, Ušjak, Dušan, Milenković, Marina, Kundaković-Vasović, Tatjana, "Inhibition of A-amylase in vitro and in vivo hypoglycemic effect of methanol extract of Alchemilla viridiflora Rothm" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S461-S462,
https://hdl.handle.net/21.15107/rcub_farfar_4580 .

TY  - JOUR
AU  - Suručić, Relja
AU  - Radović-Selgrad, Jelena
AU  - Kundaković-Vasović, Tatjana
AU  - Lazović, Vesna
AU  - Travar, Maja
AU  - Suručić, Ljiljana
AU  - Škrbić, Ranko
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4261
AB  - Since the outbreak of the COVID-19 pandemic, it has been obvious that virus infection poses
a serious threat to human health on a global scale. Certain plants, particularly those rich in polyphe-
nols, have been found to be effective antiviral agents. The effectiveness of Alchemilla viridiflora Rothm.
(Rosaceae) methanol extract to prevent contact between virus spike (S)-glycoprotein and angiotensin-
converting enzyme 2 (ACE2) and neuropilin-1 (NRP1) receptors was investigated. In vitro results
revealed that the tested samples inhibited 50% of virus-receptor binding interactions in doses of
0.18 and 0.22 mg/mL for NRP1 and ACE2, respectively. Molecular docking studies revealed that the
compounds from A. viridiflora ellagitannins class had a higher affinity for binding with S-glycoprotein
whilst flavonoid compounds more significantly interacted with the NRP1 receptor. Quercetin
3-(6”-ferulylglucoside) and pentagalloylglucose were two compounds with the highest exhibited
interfering potential for selected target receptors, with binding energies of −8.035 (S-glycoprotein)
and −7.685 kcal/mol (NRP1), respectively. Furthermore, computational studies on other SARS-CoV-2
strains resulting from mutations in the original wild strain (V483A, N501Y-K417N-E484K, N501Y,
N439K, L452R-T478K, K417N, G476S, F456L, E484K) revealed that virus internalization activity was
maintained, but with different single compound contributions.
PB  - MDPI
T2  - Molecules
T1  - In Silico and In Vitro Studies of Alchemilla viridiflora Rothm-Polyphenols' Potential for Inhibition of SARS-CoV-2 Internalization
VL  - 27
IS  - 16
DO  - 10.3390/molecules27165174
ER  - 

@article{
author = "Suručić, Relja and Radović-Selgrad, Jelena and Kundaković-Vasović, Tatjana and Lazović, Vesna and Travar, Maja and Suručić, Ljiljana and Škrbić, Ranko",
year = "2022",
abstract = "Since the outbreak of the COVID-19 pandemic, it has been obvious that virus infection poses
a serious threat to human health on a global scale. Certain plants, particularly those rich in polyphe-
nols, have been found to be effective antiviral agents. The effectiveness of Alchemilla viridiflora Rothm.
(Rosaceae) methanol extract to prevent contact between virus spike (S)-glycoprotein and angiotensin-
converting enzyme 2 (ACE2) and neuropilin-1 (NRP1) receptors was investigated. In vitro results
revealed that the tested samples inhibited 50% of virus-receptor binding interactions in doses of
0.18 and 0.22 mg/mL for NRP1 and ACE2, respectively. Molecular docking studies revealed that the
compounds from A. viridiflora ellagitannins class had a higher affinity for binding with S-glycoprotein
whilst flavonoid compounds more significantly interacted with the NRP1 receptor. Quercetin
3-(6”-ferulylglucoside) and pentagalloylglucose were two compounds with the highest exhibited
interfering potential for selected target receptors, with binding energies of −8.035 (S-glycoprotein)
and −7.685 kcal/mol (NRP1), respectively. Furthermore, computational studies on other SARS-CoV-2
strains resulting from mutations in the original wild strain (V483A, N501Y-K417N-E484K, N501Y,
N439K, L452R-T478K, K417N, G476S, F456L, E484K) revealed that virus internalization activity was
maintained, but with different single compound contributions.",
publisher = "MDPI",
journal = "Molecules",
title = "In Silico and In Vitro Studies of Alchemilla viridiflora Rothm-Polyphenols' Potential for Inhibition of SARS-CoV-2 Internalization",
volume = "27",
number = "16",
doi = "10.3390/molecules27165174"
}

Suručić, R., Radović-Selgrad, J., Kundaković-Vasović, T., Lazović, V., Travar, M., Suručić, L.,& Škrbić, R.. (2022). In Silico and In Vitro Studies of Alchemilla viridiflora Rothm-Polyphenols' Potential for Inhibition of SARS-CoV-2 Internalization. in Molecules
MDPI., 27(16).
https://doi.org/10.3390/molecules27165174

Suručić R, Radović-Selgrad J, Kundaković-Vasović T, Lazović V, Travar M, Suručić L, Škrbić R. In Silico and In Vitro Studies of Alchemilla viridiflora Rothm-Polyphenols' Potential for Inhibition of SARS-CoV-2 Internalization. in Molecules. 2022;27(16).
doi:10.3390/molecules27165174 .

Suručić, Relja, Radović-Selgrad, Jelena, Kundaković-Vasović, Tatjana, Lazović, Vesna, Travar, Maja, Suručić, Ljiljana, Škrbić, Ranko, "In Silico and In Vitro Studies of Alchemilla viridiflora Rothm-Polyphenols' Potential for Inhibition of SARS-CoV-2 Internalization" in Molecules, 27, no. 16 (2022),
https://doi.org/10.3390/molecules27165174 . .

TY  - JOUR
AU  - Radović, Jelena
AU  - Leković, Aleksandra
AU  - Damjanović, Ana B.
AU  - Kopanja, Đurđica
AU  - Dodevska, Margarita
AU  - Stanojković, Tatjana
AU  - Marinković, Tijana
AU  - Jelić, Čedomir
AU  - Kundaković-Vasović, Tatjana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5057
AB  - The composition of biologically active compounds of Calocybe gambosa (Lyophyllaceae) was analysed, and
the antioxidant and cytotoxic activities were tested in vitro. C. gambosa was low in energy, fat, and car-
bohydrates, but rich in proteins and fibres. The total polyphenol content in the extracts was low (23.08 ±
0.67 in aqueous and 24.99 ± 4.25 mg GA mg 1 of extract in methanolic extract). The methanolic extract
showed anti-DPPH radical activity with an IC 50 of 626.10 ± 25.20 mg mL 1 . The sample of C. gambosa is
rich in nucleotides and amino acids responsible for its pleasant taste. The nucleoside and 50-mono-
phosphates contents were 0.97 mg g 1 and 2.32 mg g 1 of dry mushroom, respectively. The contents of
essential and non-essential amino acids were 18.41 ± 0.06 and 41.75 ± 0.30 mg g 1 d.w., respectively. The
percentages of saturated and unsaturated fatty acids were 42.6% and 57.4%, respectively. The most
abundant water-soluble and fat-soluble vitamins were B1 and E, respectively. Cytotoxic effect of the extracts was examined against different cancer cell lines, and the best cytotoxicity was showed by the
peptide extract against colon cancer cell line LS174.
T2  - Acta Alimentaria
T1  - St. George’s mushroom, Calocybe gambosa (Fr.) Donk: A promising source of nutrients and biologically active compounds
VL  - 51
IS  - 1
SP  - 134
EP  - 144
DO  - 10.1556/066.2021.00227
ER  - 

@article{
author = "Radović, Jelena and Leković, Aleksandra and Damjanović, Ana B. and Kopanja, Đurđica and Dodevska, Margarita and Stanojković, Tatjana and Marinković, Tijana and Jelić, Čedomir and Kundaković-Vasović, Tatjana",
year = "2022",
abstract = "The composition of biologically active compounds of Calocybe gambosa (Lyophyllaceae) was analysed, and
the antioxidant and cytotoxic activities were tested in vitro. C. gambosa was low in energy, fat, and car-
bohydrates, but rich in proteins and fibres. The total polyphenol content in the extracts was low (23.08 ±
0.67 in aqueous and 24.99 ± 4.25 mg GA mg 1 of extract in methanolic extract). The methanolic extract
showed anti-DPPH radical activity with an IC 50 of 626.10 ± 25.20 mg mL 1 . The sample of C. gambosa is
rich in nucleotides and amino acids responsible for its pleasant taste. The nucleoside and 50-mono-
phosphates contents were 0.97 mg g 1 and 2.32 mg g 1 of dry mushroom, respectively. The contents of
essential and non-essential amino acids were 18.41 ± 0.06 and 41.75 ± 0.30 mg g 1 d.w., respectively. The
percentages of saturated and unsaturated fatty acids were 42.6% and 57.4%, respectively. The most
abundant water-soluble and fat-soluble vitamins were B1 and E, respectively. Cytotoxic effect of the extracts was examined against different cancer cell lines, and the best cytotoxicity was showed by the
peptide extract against colon cancer cell line LS174.",
journal = "Acta Alimentaria",
title = "St. George’s mushroom, Calocybe gambosa (Fr.) Donk: A promising source of nutrients and biologically active compounds",
volume = "51",
number = "1",
pages = "134-144",
doi = "10.1556/066.2021.00227"
}

Radović, J., Leković, A., Damjanović, A. B., Kopanja, Đ., Dodevska, M., Stanojković, T., Marinković, T., Jelić, Č.,& Kundaković-Vasović, T.. (2022). St. George’s mushroom, Calocybe gambosa (Fr.) Donk: A promising source of nutrients and biologically active compounds. in Acta Alimentaria, 51(1), 134-144.
https://doi.org/10.1556/066.2021.00227

Radović J, Leković A, Damjanović AB, Kopanja Đ, Dodevska M, Stanojković T, Marinković T, Jelić Č, Kundaković-Vasović T. St. George’s mushroom, Calocybe gambosa (Fr.) Donk: A promising source of nutrients and biologically active compounds. in Acta Alimentaria. 2022;51(1):134-144.
doi:10.1556/066.2021.00227 .

Radović, Jelena, Leković, Aleksandra, Damjanović, Ana B., Kopanja, Đurđica, Dodevska, Margarita, Stanojković, Tatjana, Marinković, Tijana, Jelić, Čedomir, Kundaković-Vasović, Tatjana, "St. George’s mushroom, Calocybe gambosa (Fr.) Donk: A promising source of nutrients and biologically active compounds" in Acta Alimentaria, 51, no. 1 (2022):134-144,
https://doi.org/10.1556/066.2021.00227 . .

TY  - CONF
AU  - Radović, Jelena
AU  - Kundaković-Vasović, Tatjana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4619
AB  - The parasol mushroom Macrolepiota procera (Scop.:Fr.) Sing, Lepiotaceae, is a highly valued edible type of fungi. Its top is an egg-shaped, gray to brown hat, covered in peal-like marks. This mushroom resides on a layer of decomposing leaves in all types of the forests, appearing solitary or in small groups from late summer to autumn. Besides good taste, it has good nutritional and medicinal value. So far, scientific studies on M. procera have shown its antihypertensive, antioxidative, antiproliferative and cytotoxic abilities. Inhibitory effect against alpha-amylase, total polyphenolic content and antiradical scavenging activity of methanol extracts prepared from dry mushroom from local market in Belgrade was the main goal of this study. Alpha-amylase inhibitory assay was assessed spectrophotometrically with 3,5-dinitrosalicylic acid reagent. Total polyphenolic content and antiradical activity in methanolic extract was determined using common Folin-Ciocalteu method and DPPH assay. Concentration of methanolic extract that inhibited 50% alpha-amylase (IC50) was 0.66 mg/mL. The content of polyphenols in methanolic extract was 13.07±0.24 mg of gallic acid/g dry extract. The concentration that neutralizes 50% DPPH radicals was 466.45±5.52 μg/mL, which was weaker antiradical activity than standard antioxidants vitamin C (IC50 3.8 μg/ mL) and quercetin (IC50 2.75 μg/mL).
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia, Belgrade
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
T1  - Anti-alpha amylase activity of Macrolepiota procera (Scop.: Fr.) Sing.
SP  - 146
EP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4619
ER  - 

@conference{
author = "Radović, Jelena and Kundaković-Vasović, Tatjana",
year = "2022",
abstract = "The parasol mushroom Macrolepiota procera (Scop.:Fr.) Sing, Lepiotaceae, is a highly valued edible type of fungi. Its top is an egg-shaped, gray to brown hat, covered in peal-like marks. This mushroom resides on a layer of decomposing leaves in all types of the forests, appearing solitary or in small groups from late summer to autumn. Besides good taste, it has good nutritional and medicinal value. So far, scientific studies on M. procera have shown its antihypertensive, antioxidative, antiproliferative and cytotoxic abilities. Inhibitory effect against alpha-amylase, total polyphenolic content and antiradical scavenging activity of methanol extracts prepared from dry mushroom from local market in Belgrade was the main goal of this study. Alpha-amylase inhibitory assay was assessed spectrophotometrically with 3,5-dinitrosalicylic acid reagent. Total polyphenolic content and antiradical activity in methanolic extract was determined using common Folin-Ciocalteu method and DPPH assay. Concentration of methanolic extract that inhibited 50% alpha-amylase (IC50) was 0.66 mg/mL. The content of polyphenols in methanolic extract was 13.07±0.24 mg of gallic acid/g dry extract. The concentration that neutralizes 50% DPPH radicals was 466.45±5.52 μg/mL, which was weaker antiradical activity than standard antioxidants vitamin C (IC50 3.8 μg/ mL) and quercetin (IC50 2.75 μg/mL).",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia, Belgrade",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts",
title = "Anti-alpha amylase activity of Macrolepiota procera (Scop.: Fr.) Sing.",
pages = "146-147",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4619"
}

Radović, J.,& Kundaković-Vasović, T.. (2022). Anti-alpha amylase activity of Macrolepiota procera (Scop.: Fr.) Sing.. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 146-147.
https://hdl.handle.net/21.15107/rcub_farfar_4619

Radović J, Kundaković-Vasović T. Anti-alpha amylase activity of Macrolepiota procera (Scop.: Fr.) Sing.. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts. 2022;:146-147.
https://hdl.handle.net/21.15107/rcub_farfar_4619 .

Radović, Jelena, Kundaković-Vasović, Tatjana, "Anti-alpha amylase activity of Macrolepiota procera (Scop.: Fr.) Sing." in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts (2022):146-147,
https://hdl.handle.net/21.15107/rcub_farfar_4619 .

TY  - CONF
AU  - Radović, Jelena
AU  - Kundaković-Vasović, Tatjana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4620
AB  - Tinder fungus, Fomes fomentarius (L.) Fr. (Polyporaceae), is a large, woody, perennial fungus. It is a plant pathogen which grows on the beech and other deciduous species as a parasite or saprophyte causing a heart rot of the wood. Some studies have shown that F. fomentarius has hypoglycemic, anti-nociceptive, anti-inflammatory, anti-infective and anti-tumor effect. Several classes of metabolites were identified: primary metabolites i.e., proteins, polysaccharides (β-glucans), polysaccharide-protein complexes, and secondary metabolites such as triterpenes and sterols, organic acids, benzofurans, coumarins, volatile components. GC/MS analysis of cyclohexane extract of fungus collected on mountain Avala in 2017 have shown the presence of sterols: ergosta-5,7,22-trien-3-ol (46.8%); ergosta-7,22-dien-3-ol (25.4%); ergosta-7,22-dien-3-on (15.9%); ergost-7-en-3-ol (fungisterol, kampesterol) (4.3%); 5,6-dihidro-3-ergosterol (5.5%). Fatty acids were also determined, including palmitic acid (C16:0) 34.0%, stearic acid (C18:0) 18.0%, linoleic acid (C18:2n6c) 10.8%, heneicosanoic acid (C21:0) (5.6%), behenic acid (C22:0) (10.9%), tricosanoic acid (C23:0) (18.4%) and lignoceric acid (C24:0) (2.3%). Cyclohexane extract has shown antibacterial activity against several bacterial strains including Helicobacter pylori and significant cytotoxic activity against HeLa cell line with an IC50 25.10±7.16 (μg/mL). These activities could be due to the presence of cytotoxic fatty acids.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia, Belgrade
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
T1  - Lipids in tinder fungus, Fomes fometarius (L.) Fr., Polyporaceae
SP  - 140
EP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4620
ER  - 

@conference{
author = "Radović, Jelena and Kundaković-Vasović, Tatjana",
year = "2022",
abstract = "Tinder fungus, Fomes fomentarius (L.) Fr. (Polyporaceae), is a large, woody, perennial fungus. It is a plant pathogen which grows on the beech and other deciduous species as a parasite or saprophyte causing a heart rot of the wood. Some studies have shown that F. fomentarius has hypoglycemic, anti-nociceptive, anti-inflammatory, anti-infective and anti-tumor effect. Several classes of metabolites were identified: primary metabolites i.e., proteins, polysaccharides (β-glucans), polysaccharide-protein complexes, and secondary metabolites such as triterpenes and sterols, organic acids, benzofurans, coumarins, volatile components. GC/MS analysis of cyclohexane extract of fungus collected on mountain Avala in 2017 have shown the presence of sterols: ergosta-5,7,22-trien-3-ol (46.8%); ergosta-7,22-dien-3-ol (25.4%); ergosta-7,22-dien-3-on (15.9%); ergost-7-en-3-ol (fungisterol, kampesterol) (4.3%); 5,6-dihidro-3-ergosterol (5.5%). Fatty acids were also determined, including palmitic acid (C16:0) 34.0%, stearic acid (C18:0) 18.0%, linoleic acid (C18:2n6c) 10.8%, heneicosanoic acid (C21:0) (5.6%), behenic acid (C22:0) (10.9%), tricosanoic acid (C23:0) (18.4%) and lignoceric acid (C24:0) (2.3%). Cyclohexane extract has shown antibacterial activity against several bacterial strains including Helicobacter pylori and significant cytotoxic activity against HeLa cell line with an IC50 25.10±7.16 (μg/mL). These activities could be due to the presence of cytotoxic fatty acids.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia, Belgrade",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts",
title = "Lipids in tinder fungus, Fomes fometarius (L.) Fr., Polyporaceae",
pages = "140-140",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4620"
}

Radović, J.,& Kundaković-Vasović, T.. (2022). Lipids in tinder fungus, Fomes fometarius (L.) Fr., Polyporaceae. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 140-140.
https://hdl.handle.net/21.15107/rcub_farfar_4620

Radović J, Kundaković-Vasović T. Lipids in tinder fungus, Fomes fometarius (L.) Fr., Polyporaceae. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts. 2022;:140-140.
https://hdl.handle.net/21.15107/rcub_farfar_4620 .

Radović, Jelena, Kundaković-Vasović, Tatjana, "Lipids in tinder fungus, Fomes fometarius (L.) Fr., Polyporaceae" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts (2022):140-140,
https://hdl.handle.net/21.15107/rcub_farfar_4620 .

TY  - JOUR
AU  - Radović, Jelena
AU  - Leković, Aleksandra
AU  - Tačić, Ana
AU  - Dodevska, Margarita
AU  - Stanojković, Tatjana
AU  - Marinković, Tijana
AU  - Jelić, Čedomir
AU  - Kundaković-Vasović, Tatjana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4324
AB  - Nutritional value and chemical composition, including the content of vitamins, fatty acids, 5'-nucleotides and nucleosides and amino acids, as well as biological activities, including antioxidant, angiotensin converting enzyme (ACE) inhibitory and cytotoxic activity of black trumpet (Craterellus cornucopioides (L.) Pers.) were tested in vitro. C. cornucopioides was low in energy, fat and carbohydrate contents, but rich in dietary fibre, especially β-glucan as well as niacin and α-tocopherol. The content of essential and non-essential free amino acids was 1.49 and 5.48 mg/g dry weight (dw). The nucleosides and 5'-nucleotides were determined at 1.84 and 3.99 mg/g dw, respectively. The share of unsaturated fatty acids (UFAs) was 75.92% with oleic acid as the major UFA. Cyclohexane and dichloromethane extracts expressed significant cytotoxic activity against selected cell lines, human epithelial cervical cancer cells (HeLa), adenocarcinomic human alveolar basal epithelial cells (A549), colorectal cancer cells (LS174) and normal MRC-5 human embryonic lung fibroblast cells (IC50 of 78.3-155.6 μg/mL). ACE inhibitory activity of the aqueous extract was strong with an IC50 of 0.74 μg/mL. It can be concluded that black trumpet is a good source of nutrients, such as vitamins, dietary fibres, amino acids, nucleotides and fatty acids, which contribute to the overall nutritional value of this fungus with potential for ACE inhibitory activity and use in anti-hypertensive diet.
PB  - Polish Academy Sciences. Institute of Animal Reproduction and Food Research
T2  - Polish Journal of Food and Nutrition Sciences
T1  - Black Trumpet, Craterellus cornucopioides (L.) Pers.: Culinary Mushroom with Angiotensin Converting Enzyme Inhibitory and Cytotoxic Activity
VL  - 72
IS  - 2
SP  - 171
EP  - 181
DO  - 10.31883/pjfns/149914
ER  - 

@article{
author = "Radović, Jelena and Leković, Aleksandra and Tačić, Ana and Dodevska, Margarita and Stanojković, Tatjana and Marinković, Tijana and Jelić, Čedomir and Kundaković-Vasović, Tatjana",
year = "2022",
abstract = "Nutritional value and chemical composition, including the content of vitamins, fatty acids, 5'-nucleotides and nucleosides and amino acids, as well as biological activities, including antioxidant, angiotensin converting enzyme (ACE) inhibitory and cytotoxic activity of black trumpet (Craterellus cornucopioides (L.) Pers.) were tested in vitro. C. cornucopioides was low in energy, fat and carbohydrate contents, but rich in dietary fibre, especially β-glucan as well as niacin and α-tocopherol. The content of essential and non-essential free amino acids was 1.49 and 5.48 mg/g dry weight (dw). The nucleosides and 5'-nucleotides were determined at 1.84 and 3.99 mg/g dw, respectively. The share of unsaturated fatty acids (UFAs) was 75.92% with oleic acid as the major UFA. Cyclohexane and dichloromethane extracts expressed significant cytotoxic activity against selected cell lines, human epithelial cervical cancer cells (HeLa), adenocarcinomic human alveolar basal epithelial cells (A549), colorectal cancer cells (LS174) and normal MRC-5 human embryonic lung fibroblast cells (IC50 of 78.3-155.6 μg/mL). ACE inhibitory activity of the aqueous extract was strong with an IC50 of 0.74 μg/mL. It can be concluded that black trumpet is a good source of nutrients, such as vitamins, dietary fibres, amino acids, nucleotides and fatty acids, which contribute to the overall nutritional value of this fungus with potential for ACE inhibitory activity and use in anti-hypertensive diet.",
publisher = "Polish Academy Sciences. Institute of Animal Reproduction and Food Research",
journal = "Polish Journal of Food and Nutrition Sciences",
title = "Black Trumpet, Craterellus cornucopioides (L.) Pers.: Culinary Mushroom with Angiotensin Converting Enzyme Inhibitory and Cytotoxic Activity",
volume = "72",
number = "2",
pages = "171-181",
doi = "10.31883/pjfns/149914"
}

Radović, J., Leković, A., Tačić, A., Dodevska, M., Stanojković, T., Marinković, T., Jelić, Č.,& Kundaković-Vasović, T.. (2022). Black Trumpet, Craterellus cornucopioides (L.) Pers.: Culinary Mushroom with Angiotensin Converting Enzyme Inhibitory and Cytotoxic Activity. in Polish Journal of Food and Nutrition Sciences
Polish Academy Sciences. Institute of Animal Reproduction and Food Research., 72(2), 171-181.
https://doi.org/10.31883/pjfns/149914

Radović J, Leković A, Tačić A, Dodevska M, Stanojković T, Marinković T, Jelić Č, Kundaković-Vasović T. Black Trumpet, Craterellus cornucopioides (L.) Pers.: Culinary Mushroom with Angiotensin Converting Enzyme Inhibitory and Cytotoxic Activity. in Polish Journal of Food and Nutrition Sciences. 2022;72(2):171-181.
doi:10.31883/pjfns/149914 .

Radović, Jelena, Leković, Aleksandra, Tačić, Ana, Dodevska, Margarita, Stanojković, Tatjana, Marinković, Tijana, Jelić, Čedomir, Kundaković-Vasović, Tatjana, "Black Trumpet, Craterellus cornucopioides (L.) Pers.: Culinary Mushroom with Angiotensin Converting Enzyme Inhibitory and Cytotoxic Activity" in Polish Journal of Food and Nutrition Sciences, 72, no. 2 (2022):171-181,
https://doi.org/10.31883/pjfns/149914 . .

TY  - JOUR
AU  - Radović, Jelena
AU  - Suručić, Relja
AU  - Niketić, Marjan
AU  - Kundaković-Vasović, Tatjana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4114
AB  - Alchemilla viridiflora Rothm., Rosaceae is a herbaceous plant widespread in central Greece, Bulgaria, North Macedonia and Serbia with Kosovo. Liquid chromatography-mass spectrometry analysis leads to the identification of 20 compounds in methanol extract, mainly ellagitannins and flavonoid glycosides. Given that various plant extracts have traditionally been used to treat hypertension and that some of the analyzed methanol extract constituents have beneficial cardiovascular effects, we hypothesized that some of these effects are achieved by inhibiting angiotensin I-converting enzyme (ACE). The dose- dependent ACE inhibitory activities of A. viridiflora and miquelianin were observed with an IC 50 of 2.51 ± 0.00 μg/mL of A. viridiflora extract compared to the IC 50 of 5.4139 ± 0.00 μM for miquelianin. The contribution of the single compounds to the tested activity was further analyzed through the in silico experimental approach. Computational docking results showed that tiliroside, ellagic acid pentose and galloyl-hexahydroxydiphenoyl-glucose exhibited even better binding affinity for the ACE active site than miquelianin, for which ACE activity was confirmed by an in vitro assay.
PB  - Springer
T2  - Molecular and Cellular Biochemistry
T1  - Alchemilla viridiflora Rothm.: the potent natural inhibitor of angiotensin I-converting enzyme
VL  - 477
SP  - 1893
EP  - 1903
DO  - 10.1007/s11010-022-04410-7
ER  - 

@article{
author = "Radović, Jelena and Suručić, Relja and Niketić, Marjan and Kundaković-Vasović, Tatjana",
year = "2022",
abstract = "Alchemilla viridiflora Rothm., Rosaceae is a herbaceous plant widespread in central Greece, Bulgaria, North Macedonia and Serbia with Kosovo. Liquid chromatography-mass spectrometry analysis leads to the identification of 20 compounds in methanol extract, mainly ellagitannins and flavonoid glycosides. Given that various plant extracts have traditionally been used to treat hypertension and that some of the analyzed methanol extract constituents have beneficial cardiovascular effects, we hypothesized that some of these effects are achieved by inhibiting angiotensin I-converting enzyme (ACE). The dose- dependent ACE inhibitory activities of A. viridiflora and miquelianin were observed with an IC 50 of 2.51 ± 0.00 μg/mL of A. viridiflora extract compared to the IC 50 of 5.4139 ± 0.00 μM for miquelianin. The contribution of the single compounds to the tested activity was further analyzed through the in silico experimental approach. Computational docking results showed that tiliroside, ellagic acid pentose and galloyl-hexahydroxydiphenoyl-glucose exhibited even better binding affinity for the ACE active site than miquelianin, for which ACE activity was confirmed by an in vitro assay.",
publisher = "Springer",
journal = "Molecular and Cellular Biochemistry",
title = "Alchemilla viridiflora Rothm.: the potent natural inhibitor of angiotensin I-converting enzyme",
volume = "477",
pages = "1893-1903",
doi = "10.1007/s11010-022-04410-7"
}

Radović, J., Suručić, R., Niketić, M.,& Kundaković-Vasović, T.. (2022). Alchemilla viridiflora Rothm.: the potent natural inhibitor of angiotensin I-converting enzyme. in Molecular and Cellular Biochemistry
Springer., 477, 1893-1903.
https://doi.org/10.1007/s11010-022-04410-7

Radović J, Suručić R, Niketić M, Kundaković-Vasović T. Alchemilla viridiflora Rothm.: the potent natural inhibitor of angiotensin I-converting enzyme. in Molecular and Cellular Biochemistry. 2022;477:1893-1903.
doi:10.1007/s11010-022-04410-7 .

Radović, Jelena, Suručić, Relja, Niketić, Marjan, Kundaković-Vasović, Tatjana, "Alchemilla viridiflora Rothm.: the potent natural inhibitor of angiotensin I-converting enzyme" in Molecular and Cellular Biochemistry, 477 (2022):1893-1903,
https://doi.org/10.1007/s11010-022-04410-7 . .

TY  - JOUR
AU  - Punoševac, Milka
AU  - Radović, Jelena
AU  - Leković, Aleksandra
AU  - Kundaković-Vasović, Tatjana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3919
AB  - Parsley is a biennial aromatic plant from the Apiaceae family, which is characterized by an unbranched root, pinnately divided leaves, umbels and schizocarp. It contains essential oil in all parts, with phenylpropane and terpene compounds as main components. It is rich in flavonoids and other polyphenolic compounds, containing furanocoumarins, carotenoids, polyacetylenes, and its leaves are a source of vitamins and minerals. The chemical composition of parsley depends on a number of factors, so it differs not only in different parts and varieties of the plant, but also in different samples of the same parts of one variety. The most important parsley compounds are myristicin,  apiol,  1-allyl-2,3,4,5-tetramethoxybenzene,  β-phellandrene,  1,3,8-p-menthatriene,                 β-pinene, terpinolene, apiin, oxypeucedanin and falcarinol. Parsley has a long tradition of use in the treatment of urinary tract disorders, and modern in vitro and in vivo studies reveal numerous effects  of  various  parsley  preparations  such  as  diuretic,  antiurolithiasis,  hypouricemic, hypolipidemic,  hypoglycemic,  hypotensive,  antioxidant,  anti-inflammatory  and  antiplatelet effect. Today, apart from its medical application, parsley is one of the most commonly used culinary herbs.
AB  - Peršun je dvogodišnja aromatična biljka iz familije Apiaceae koju karakterišu nerazgranat koren, perasto deljeni listovi, štitaste cvasti i plod šizokarpijum. U svim delovima sadrži etarsko ulje, čije su glavne komponente fenilpropanska i terpenska jedinjenja. Bogat je flavonoidima i drugim polifenolnim jedinjenjima, sadrži furanokumarine, karotenoide, poliacetilene, a listovi su izvor vitamina i minerala. Hemijski sastav peršuna zavisi od brojnih faktora, pa tako postoje razlike u sastavu između ne samo različitih delova i varijeteta biljke, već i različitih uzoraka istih delova jednog varijeteta. Najzastupljenija jedinjenja peršuna su miristicin, apiol, 1-alil-2,3,4,5tetrametoksibenzen, b-felandren, 1,3,8-p-mentatrien, b-pinen, terpinolen, apiin, oksipeucedanin i falkarinol. Peršun ima dugu tradiciju upotrebe u tretmanu poremećaja funkcije urinarnog trakta, a savremena in vitro i in vivo ispitivanja otkrivaju i brojne efekte koje poseduju različiti preparati peršuna kao što su diuretički, antiurolitijazni, hipourikemijski, hipolipidemijski, hipoglikemijski, hipotenzivni, antioksidativni, antiinflamatorni, antimikrobni i antiagregacioni. Danas, pored medicinske primene, peršun je jedna od najkorišćenijih začinskih biljaka.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - A review of botanical characteristics, chemical composition, pharmacological activity and use of parsley
T1  - Pregled botaničkih karakteristika, hemijskog sastava, farmakološke aktivnosti i primene peršuna
VL  - 71
IS  - 3
SP  - 177
EP  - 196
DO  - 10.5937/arhfarm71‐30800
ER  - 

@article{
author = "Punoševac, Milka and Radović, Jelena and Leković, Aleksandra and Kundaković-Vasović, Tatjana",
year = "2021",
abstract = "Parsley is a biennial aromatic plant from the Apiaceae family, which is characterized by an unbranched root, pinnately divided leaves, umbels and schizocarp. It contains essential oil in all parts, with phenylpropane and terpene compounds as main components. It is rich in flavonoids and other polyphenolic compounds, containing furanocoumarins, carotenoids, polyacetylenes, and its leaves are a source of vitamins and minerals. The chemical composition of parsley depends on a number of factors, so it differs not only in different parts and varieties of the plant, but also in different samples of the same parts of one variety. The most important parsley compounds are myristicin,  apiol,  1-allyl-2,3,4,5-tetramethoxybenzene,  β-phellandrene,  1,3,8-p-menthatriene,                 β-pinene, terpinolene, apiin, oxypeucedanin and falcarinol. Parsley has a long tradition of use in the treatment of urinary tract disorders, and modern in vitro and in vivo studies reveal numerous effects  of  various  parsley  preparations  such  as  diuretic,  antiurolithiasis,  hypouricemic, hypolipidemic,  hypoglycemic,  hypotensive,  antioxidant,  anti-inflammatory  and  antiplatelet effect. Today, apart from its medical application, parsley is one of the most commonly used culinary herbs., Peršun je dvogodišnja aromatična biljka iz familije Apiaceae koju karakterišu nerazgranat koren, perasto deljeni listovi, štitaste cvasti i plod šizokarpijum. U svim delovima sadrži etarsko ulje, čije su glavne komponente fenilpropanska i terpenska jedinjenja. Bogat je flavonoidima i drugim polifenolnim jedinjenjima, sadrži furanokumarine, karotenoide, poliacetilene, a listovi su izvor vitamina i minerala. Hemijski sastav peršuna zavisi od brojnih faktora, pa tako postoje razlike u sastavu između ne samo različitih delova i varijeteta biljke, već i različitih uzoraka istih delova jednog varijeteta. Najzastupljenija jedinjenja peršuna su miristicin, apiol, 1-alil-2,3,4,5tetrametoksibenzen, b-felandren, 1,3,8-p-mentatrien, b-pinen, terpinolen, apiin, oksipeucedanin i falkarinol. Peršun ima dugu tradiciju upotrebe u tretmanu poremećaja funkcije urinarnog trakta, a savremena in vitro i in vivo ispitivanja otkrivaju i brojne efekte koje poseduju različiti preparati peršuna kao što su diuretički, antiurolitijazni, hipourikemijski, hipolipidemijski, hipoglikemijski, hipotenzivni, antioksidativni, antiinflamatorni, antimikrobni i antiagregacioni. Danas, pored medicinske primene, peršun je jedna od najkorišćenijih začinskih biljaka.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "A review of botanical characteristics, chemical composition, pharmacological activity and use of parsley, Pregled botaničkih karakteristika, hemijskog sastava, farmakološke aktivnosti i primene peršuna",
volume = "71",
number = "3",
pages = "177-196",
doi = "10.5937/arhfarm71‐30800"
}

Punoševac, M., Radović, J., Leković, A.,& Kundaković-Vasović, T.. (2021). A review of botanical characteristics, chemical composition, pharmacological activity and use of parsley. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 71(3), 177-196.
https://doi.org/10.5937/arhfarm71‐30800

Punoševac M, Radović J, Leković A, Kundaković-Vasović T. A review of botanical characteristics, chemical composition, pharmacological activity and use of parsley. in Arhiv za farmaciju. 2021;71(3):177-196.
doi:10.5937/arhfarm71‐30800 .

Punoševac, Milka, Radović, Jelena, Leković, Aleksandra, Kundaković-Vasović, Tatjana, "A review of botanical characteristics, chemical composition, pharmacological activity and use of parsley" in Arhiv za farmaciju, 71, no. 3 (2021):177-196,
https://doi.org/10.5937/arhfarm71‐30800 . .

TY  - CONF
AU  - Kundaković-Vasović, Tatjana
AU  - Radović, Jelena
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5058
AB  - UVOD I CILJ Jestive gljive su važan izvor hranljivih i bioaktivnih jedinjenja sa različitim farmakološkim aktivnostima. Ekstrakti gljiva Fomes fomentarius-FF, Laetiporus sulphureus-LS, Cantarellus cibarius-CC, Cratherellus cornucopioides-CrC i Calocibe gambosa-CG bile su predmet hemijske analize i in vitro ispitivanja nekoliko aktivnosti: antioksidantne, citotoksične, antibakterijske i ACE inhibitorne aktivnosti. ...
AB  - INTRODUCTION AND OBJECTIVE The edible mushrooms are important source of nutritive and bioactive compounds with different pharmacological activities. Extracts of fungi Fomes fomentarius-FF, Laetiporus sulphureus-LS, Cantarellus cibarius-CC, Cratherellus cornucopioides-CrC and Calocybe gambosa-CG, were the subject of chemical analysis and in vitro testing of several activities: antioxidant, cytotoxic, antibacterial activity, ACE inhibitory activity. ...
PB  - Komora magistara farmacije Federacije Bosne i Hercegovine
PB  - Farmaceutsko društvo Republike Srpske
C3  - 4. kongres farmaceuta u Bosni i Hercegovini sa međunarodnim učešćem, "Novi trendovi u farmaciji", 10-13.10.2019., Sarajevo, Knjiga sažetaka
T1  - Nutritivni i bioaktivni sastojci odabranih gljiva iz familija Polyporaceae, Cantharellaceae i Lyophyllaceae
SP  - 196
EP  - 197
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5058
ER  - 

@conference{
author = "Kundaković-Vasović, Tatjana and Radović, Jelena",
year = "2019",
abstract = "UVOD I CILJ Jestive gljive su važan izvor hranljivih i bioaktivnih jedinjenja sa različitim farmakološkim aktivnostima. Ekstrakti gljiva Fomes fomentarius-FF, Laetiporus sulphureus-LS, Cantarellus cibarius-CC, Cratherellus cornucopioides-CrC i Calocibe gambosa-CG bile su predmet hemijske analize i in vitro ispitivanja nekoliko aktivnosti: antioksidantne, citotoksične, antibakterijske i ACE inhibitorne aktivnosti. ..., INTRODUCTION AND OBJECTIVE The edible mushrooms are important source of nutritive and bioactive compounds with different pharmacological activities. Extracts of fungi Fomes fomentarius-FF, Laetiporus sulphureus-LS, Cantarellus cibarius-CC, Cratherellus cornucopioides-CrC and Calocybe gambosa-CG, were the subject of chemical analysis and in vitro testing of several activities: antioxidant, cytotoxic, antibacterial activity, ACE inhibitory activity. ...",
publisher = "Komora magistara farmacije Federacije Bosne i Hercegovine, Farmaceutsko društvo Republike Srpske",
journal = "4. kongres farmaceuta u Bosni i Hercegovini sa međunarodnim učešćem, "Novi trendovi u farmaciji", 10-13.10.2019., Sarajevo, Knjiga sažetaka",
title = "Nutritivni i bioaktivni sastojci odabranih gljiva iz familija Polyporaceae, Cantharellaceae i Lyophyllaceae",
pages = "196-197",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5058"
}

Kundaković-Vasović, T.,& Radović, J.. (2019). Nutritivni i bioaktivni sastojci odabranih gljiva iz familija Polyporaceae, Cantharellaceae i Lyophyllaceae. in 4. kongres farmaceuta u Bosni i Hercegovini sa međunarodnim učešćem, "Novi trendovi u farmaciji", 10-13.10.2019., Sarajevo, Knjiga sažetaka
Komora magistara farmacije Federacije Bosne i Hercegovine., 196-197.
https://hdl.handle.net/21.15107/rcub_farfar_5058

Kundaković-Vasović T, Radović J. Nutritivni i bioaktivni sastojci odabranih gljiva iz familija Polyporaceae, Cantharellaceae i Lyophyllaceae. in 4. kongres farmaceuta u Bosni i Hercegovini sa međunarodnim učešćem, "Novi trendovi u farmaciji", 10-13.10.2019., Sarajevo, Knjiga sažetaka. 2019;:196-197.
https://hdl.handle.net/21.15107/rcub_farfar_5058 .

Kundaković-Vasović, Tatjana, Radović, Jelena, "Nutritivni i bioaktivni sastojci odabranih gljiva iz familija Polyporaceae, Cantharellaceae i Lyophyllaceae" in 4. kongres farmaceuta u Bosni i Hercegovini sa međunarodnim učešćem, "Novi trendovi u farmaciji", 10-13.10.2019., Sarajevo, Knjiga sažetaka (2019):196-197,
https://hdl.handle.net/21.15107/rcub_farfar_5058 .

TY  - CONF
AU  - Radović, Jelena
AU  - Dodevska, Margarita
AU  - Kopanja, Đurđica
AU  - Kundaković-Vasović, Tatjana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5309
AB  - St. George's mushroom, Calocybe gambosa, is an edible mushroom from the family Lyophyllaceae. It is widespread in Europe. The colour of the mushroom varies from white, yellow to greyish-brown. ...
PB  - Phytochemical Society of Europe
PB  - Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
C3  - Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts
T1  - Chemical and nutritive composition of St. George’mushroom, Calocybe gambosa (Fr.) Donk
SP  - 212
EP  - 212
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5309
ER  - 

@conference{
author = "Radović, Jelena and Dodevska, Margarita and Kopanja, Đurđica and Kundaković-Vasović, Tatjana",
year = "2019",
abstract = "St. George's mushroom, Calocybe gambosa, is an edible mushroom from the family Lyophyllaceae. It is widespread in Europe. The colour of the mushroom varies from white, yellow to greyish-brown. ...",
publisher = "Phytochemical Society of Europe, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal",
journal = "Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts",
title = "Chemical and nutritive composition of St. George’mushroom, Calocybe gambosa (Fr.) Donk",
pages = "212-212",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5309"
}

Radović, J., Dodevska, M., Kopanja, Đ.,& Kundaković-Vasović, T.. (2019). Chemical and nutritive composition of St. George’mushroom, Calocybe gambosa (Fr.) Donk. in Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts
Phytochemical Society of Europe., 212-212.
https://hdl.handle.net/21.15107/rcub_farfar_5309

Radović J, Dodevska M, Kopanja Đ, Kundaković-Vasović T. Chemical and nutritive composition of St. George’mushroom, Calocybe gambosa (Fr.) Donk. in Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts. 2019;:212-212.
https://hdl.handle.net/21.15107/rcub_farfar_5309 .

Radović, Jelena, Dodevska, Margarita, Kopanja, Đurđica, Kundaković-Vasović, Tatjana, "Chemical and nutritive composition of St. George’mushroom, Calocybe gambosa (Fr.) Donk" in Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts (2019):212-212,
https://hdl.handle.net/21.15107/rcub_farfar_5309 .

TY  - CONF
AU  - Arsenijević, Jelena
AU  - Radović, Jelena
AU  - Stanojković, Tatjana
AU  - Ranđelović, Vladimir
AU  - Kundaković-Vasović, Tatjana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5308
AB  - Rumex balcanicus Rech.f. (Polygonaceae) is an endemic species that inhabits central parts of Serbia and Kosovo. Recent studies have demonstrated that the isolates of some Rumex species exhibited anti-inflammatory, antioxidant, anticancer, antidiabetic and antimicrobial activities, and aerial parts of some species are used as food. ...
PB  - Phytochemical Society of Europe
PB  - Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
C3  - Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts
T1  - Chemical analysis and cytotoxicity of Rumex balcanicus Rech. f. (Polygonaceae)
SP  - 169
EP  - 169
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5308
ER  - 

@conference{
author = "Arsenijević, Jelena and Radović, Jelena and Stanojković, Tatjana and Ranđelović, Vladimir and Kundaković-Vasović, Tatjana",
year = "2019",
abstract = "Rumex balcanicus Rech.f. (Polygonaceae) is an endemic species that inhabits central parts of Serbia and Kosovo. Recent studies have demonstrated that the isolates of some Rumex species exhibited anti-inflammatory, antioxidant, anticancer, antidiabetic and antimicrobial activities, and aerial parts of some species are used as food. ...",
publisher = "Phytochemical Society of Europe, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal",
journal = "Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts",
title = "Chemical analysis and cytotoxicity of Rumex balcanicus Rech. f. (Polygonaceae)",
pages = "169-169",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5308"
}

Arsenijević, J., Radović, J., Stanojković, T., Ranđelović, V.,& Kundaković-Vasović, T.. (2019). Chemical analysis and cytotoxicity of Rumex balcanicus Rech. f. (Polygonaceae). in Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts
Phytochemical Society of Europe., 169-169.
https://hdl.handle.net/21.15107/rcub_farfar_5308

Arsenijević J, Radović J, Stanojković T, Ranđelović V, Kundaković-Vasović T. Chemical analysis and cytotoxicity of Rumex balcanicus Rech. f. (Polygonaceae). in Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts. 2019;:169-169.
https://hdl.handle.net/21.15107/rcub_farfar_5308 .

Arsenijević, Jelena, Radović, Jelena, Stanojković, Tatjana, Ranđelović, Vladimir, Kundaković-Vasović, Tatjana, "Chemical analysis and cytotoxicity of Rumex balcanicus Rech. f. (Polygonaceae)" in Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts (2019):169-169,
https://hdl.handle.net/21.15107/rcub_farfar_5308 .

TY  - CONF
AU  - Radović, Jelena
AU  - Milenković, Marina
AU  - Ranđelović, Vladimir
AU  - Kundaković-Vasović, Tatjana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5295
AB  - Recent studies demonstrated that Rumex species exhibit various
pharmacological activities, such as antiinflamatory, antioxidant, anticancer,
antimicrobial, but there are almost no data on pharmacological activities of Balcan
endemic species Rumex balcanicus. Thus, the aim was to examine antioxidant
activity, using DPPH and FRAP method, antimicrobial activity via broth
microdilution method and α-amylase inhibitory activity using DNS reagent of
aqueous and methanol extracts of roots and aerial parts of R. balcanicus, collected on
Vlasina Lake in 2014. All tested extracts exhibited high antioxidant activity which
has been correlated with the high content of polyphenols. Aqueous extracts exhibited
lower activity compared to methanol extracts. Methanol extract ofR. balcanicus roots
showed the highest activity, both in DPPH (IC50= 9.57 μg/mL) and FRAP test
(IC50=3.98 μmol Fe/mg of extract), while aqueous extract of aerial parts showed the
lowest activity (IC50= 24.73 μg/mL; IC50=2.11 μmol Fe/mg). Only methanol extract
of aerial parts showed α-amylase inhibitory activity with IC50=252 μg/mL. All
extracts had low antimicrobial activity against almost all tested bacterial strains except
against Bacillus subtilis. Positive results obtained in this study show that R. balcanisus
contains potential as a new raw material and for the development of drugs efficient in
treating diabetes.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Pharmacological activity of Rumex balcanicus Rech. F. (Polygonaceae)
SP  - 159
EP  - 159
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5295
ER  - 

@conference{
author = "Radović, Jelena and Milenković, Marina and Ranđelović, Vladimir and Kundaković-Vasović, Tatjana",
year = "2019",
abstract = "Recent studies demonstrated that Rumex species exhibit various
pharmacological activities, such as antiinflamatory, antioxidant, anticancer,
antimicrobial, but there are almost no data on pharmacological activities of Balcan
endemic species Rumex balcanicus. Thus, the aim was to examine antioxidant
activity, using DPPH and FRAP method, antimicrobial activity via broth
microdilution method and α-amylase inhibitory activity using DNS reagent of
aqueous and methanol extracts of roots and aerial parts of R. balcanicus, collected on
Vlasina Lake in 2014. All tested extracts exhibited high antioxidant activity which
has been correlated with the high content of polyphenols. Aqueous extracts exhibited
lower activity compared to methanol extracts. Methanol extract ofR. balcanicus roots
showed the highest activity, both in DPPH (IC50= 9.57 μg/mL) and FRAP test
(IC50=3.98 μmol Fe/mg of extract), while aqueous extract of aerial parts showed the
lowest activity (IC50= 24.73 μg/mL; IC50=2.11 μmol Fe/mg). Only methanol extract
of aerial parts showed α-amylase inhibitory activity with IC50=252 μg/mL. All
extracts had low antimicrobial activity against almost all tested bacterial strains except
against Bacillus subtilis. Positive results obtained in this study show that R. balcanisus
contains potential as a new raw material and for the development of drugs efficient in
treating diabetes.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Pharmacological activity of Rumex balcanicus Rech. F. (Polygonaceae)",
pages = "159-159",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5295"
}

Radović, J., Milenković, M., Ranđelović, V.,& Kundaković-Vasović, T.. (2019). Pharmacological activity of Rumex balcanicus Rech. F. (Polygonaceae). in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 159-159.
https://hdl.handle.net/21.15107/rcub_farfar_5295

Radović J, Milenković M, Ranđelović V, Kundaković-Vasović T. Pharmacological activity of Rumex balcanicus Rech. F. (Polygonaceae). in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:159-159.
https://hdl.handle.net/21.15107/rcub_farfar_5295 .

Radović, Jelena, Milenković, Marina, Ranđelović, Vladimir, Kundaković-Vasović, Tatjana, "Pharmacological activity of Rumex balcanicus Rech. F. (Polygonaceae)" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):159-159,
https://hdl.handle.net/21.15107/rcub_farfar_5295 .

TY  - CONF
AU  - Opačić-Galić, Vanja
AU  - Pavlica, Dušan
AU  - Kundaković-Vasović, Tatjana
AU  - Radović, Jelena
AU  - Popović Bajić, Marijana
AU  - Mitić-Ćulafić, Dragana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5222
PB  - European Society of Endodontology (ESE)
C3  - 19th Biennial ESE Congress, “Vienna calling – experience the sound of Endodontology”, September 12 - 14, 2019, Vienna, Austria
T1  - Antimicrobial potential of herbal tinctures on Enterococcus faecalis
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5222
ER  - 

@conference{
author = "Opačić-Galić, Vanja and Pavlica, Dušan and Kundaković-Vasović, Tatjana and Radović, Jelena and Popović Bajić, Marijana and Mitić-Ćulafić, Dragana",
year = "2019",
publisher = "European Society of Endodontology (ESE)",
journal = "19th Biennial ESE Congress, “Vienna calling – experience the sound of Endodontology”, September 12 - 14, 2019, Vienna, Austria",
title = "Antimicrobial potential of herbal tinctures on Enterococcus faecalis",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5222"
}

Opačić-Galić, V., Pavlica, D., Kundaković-Vasović, T., Radović, J., Popović Bajić, M.,& Mitić-Ćulafić, D.. (2019). Antimicrobial potential of herbal tinctures on Enterococcus faecalis. in 19th Biennial ESE Congress, “Vienna calling – experience the sound of Endodontology”, September 12 - 14, 2019, Vienna, Austria
European Society of Endodontology (ESE)..
https://hdl.handle.net/21.15107/rcub_farfar_5222

Opačić-Galić V, Pavlica D, Kundaković-Vasović T, Radović J, Popović Bajić M, Mitić-Ćulafić D. Antimicrobial potential of herbal tinctures on Enterococcus faecalis. in 19th Biennial ESE Congress, “Vienna calling – experience the sound of Endodontology”, September 12 - 14, 2019, Vienna, Austria. 2019;.
https://hdl.handle.net/21.15107/rcub_farfar_5222 .

Opačić-Galić, Vanja, Pavlica, Dušan, Kundaković-Vasović, Tatjana, Radović, Jelena, Popović Bajić, Marijana, Mitić-Ćulafić, Dragana, "Antimicrobial potential of herbal tinctures on Enterococcus faecalis" in 19th Biennial ESE Congress, “Vienna calling – experience the sound of Endodontology”, September 12 - 14, 2019, Vienna, Austria (2019),
https://hdl.handle.net/21.15107/rcub_farfar_5222 .

TY  - JOUR
AU  - Kundaković-Vasović, Tatjana
AU  - Radović, Jelena
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3487
AB  - Herbal medicinal products are very important for cough therapy and commonly used in both self-medication and doctor's recommendation. Herbal drugs used in cough therapy can be divided into expectorant and demulcent herbal drugs (drugs containing heteropolysaccharides, most commonly mucilages). Clinical studies have confirmed the efficacy of ivy leaf extracts and fixed combinations of thyme herb and primrose roots extracts as expectorants in productive cough, while for the most other herbal drugs and their preparations used in cough therapy only traditional use is documented. Each herbal drug used in cough therapy has its specific application restrictions (contraindications, special warnings, precautions, interactions, and adverse effects).
AB  - Biljni lekoviti proizvodi su veoma značajni za terapiju kašlja i često se koriste, kako u samomedikaciji, tako i po preporuci lekara. Biljne droge koje se koriste u terapiji kašlja se mogu podeliti na droge ekspektorantnog delovanja i demulcentna sredstva, odnosno droge koje sadrže heteropolisaharide, najčešće sluzi. Kliničke studije su potvrdile efikasnost primene ekstrakata herbe bršljana i fiksnih kombinacija herbe timijana i korena jagorčevine kao ekspektoranasa kod produktivnog kašlja, dok je za veći broj drugih biljnih droga i njihovih preparata koji se koriste u terapiji kašlja, dokumentovana samo tradicionalna primena. Svaka biljna droga koja se koristi u terapiji kašlja ima svoja specifična ograničenja za primenu (kontraindikacije, specijalna upozorenja, mere opreza, interakcije i neželjena dejstva).
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Biljni lekoviti proizvodi u terapiji kašlja povezanog sa prehladom
T1  - Herbal medicinal products in the treatment of cough associated with colds
VL  - 69
IS  - 4
SP  - 270
EP  - 285
DO  - 10.5937/arhfarm1904270K
ER  - 

@article{
author = "Kundaković-Vasović, Tatjana and Radović, Jelena",
year = "2019",
abstract = "Herbal medicinal products are very important for cough therapy and commonly used in both self-medication and doctor's recommendation. Herbal drugs used in cough therapy can be divided into expectorant and demulcent herbal drugs (drugs containing heteropolysaccharides, most commonly mucilages). Clinical studies have confirmed the efficacy of ivy leaf extracts and fixed combinations of thyme herb and primrose roots extracts as expectorants in productive cough, while for the most other herbal drugs and their preparations used in cough therapy only traditional use is documented. Each herbal drug used in cough therapy has its specific application restrictions (contraindications, special warnings, precautions, interactions, and adverse effects)., Biljni lekoviti proizvodi su veoma značajni za terapiju kašlja i često se koriste, kako u samomedikaciji, tako i po preporuci lekara. Biljne droge koje se koriste u terapiji kašlja se mogu podeliti na droge ekspektorantnog delovanja i demulcentna sredstva, odnosno droge koje sadrže heteropolisaharide, najčešće sluzi. Kliničke studije su potvrdile efikasnost primene ekstrakata herbe bršljana i fiksnih kombinacija herbe timijana i korena jagorčevine kao ekspektoranasa kod produktivnog kašlja, dok je za veći broj drugih biljnih droga i njihovih preparata koji se koriste u terapiji kašlja, dokumentovana samo tradicionalna primena. Svaka biljna droga koja se koristi u terapiji kašlja ima svoja specifična ograničenja za primenu (kontraindikacije, specijalna upozorenja, mere opreza, interakcije i neželjena dejstva).",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Biljni lekoviti proizvodi u terapiji kašlja povezanog sa prehladom, Herbal medicinal products in the treatment of cough associated with colds",
volume = "69",
number = "4",
pages = "270-285",
doi = "10.5937/arhfarm1904270K"
}

Kundaković-Vasović, T.,& Radović, J.. (2019). Biljni lekoviti proizvodi u terapiji kašlja povezanog sa prehladom. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 69(4), 270-285.
https://doi.org/10.5937/arhfarm1904270K

Kundaković-Vasović T, Radović J. Biljni lekoviti proizvodi u terapiji kašlja povezanog sa prehladom. in Arhiv za farmaciju. 2019;69(4):270-285.
doi:10.5937/arhfarm1904270K .

Kundaković-Vasović, Tatjana, Radović, Jelena, "Biljni lekoviti proizvodi u terapiji kašlja povezanog sa prehladom" in Arhiv za farmaciju, 69, no. 4 (2019):270-285,
https://doi.org/10.5937/arhfarm1904270K . .

TY  - JOUR
AU  - Milović, Sanja
AU  - Stanković, Ivan
AU  - Nikolić, Dejan
AU  - Radović, Jelena
AU  - Kolundžić, Marina
AU  - Nikolić, Vesna
AU  - Stanojković, Tatjana
AU  - Petović, Slavica
AU  - Kundaković-Vasović, Tatjana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3468
AB  - Three seaweeds (Halimeda tuna, Codium bursa and Cystoseira barbata) and one seagrass (Cymodocea nodosa) were collected from the Coast of Montenegro, Gulf of Boka Kotorska and their chemical analysis was performed. In seagrass C. nodosa, three phenolic compounds were identified (diosmetin 7-sulfate, caftaric and coutaric acid). The content of β-glucan, fatty acids, sterols and micro- and macro-elements were investigated among all samples. The highest content of β-glucan was detected in C. nodosa seagrass (13.04±0.42 g/100 g). The highest polyunsaturated fatty acids (PUFAs) level was reported in C. barbata, the brown alga (7.157 mg/g), which also had the significant sterol content (fucosterol, 21.76±0.1 μg/g). Green algae, C. bursa and H. tuna, showed the highest level of sterols (β-sitosterol, 95.21±0.16 μg/g and 73.90±0.08 μg/g, respectively). H. tuna had the highest content of calcium (Ca) in amount of 55125 μg/g. In C. bursa, C. barbata and C. nodosa, the Na/K ratio was low (0.43, 0.46 and 0.69, respectively).
PB  - Wiley
T2  - Chemistry and Biodiversity
T1  - Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro
VL  - 16
IS  - 10
SP  - 1
EP  - 11
DO  - 10.1002/cbdv.201900327
ER  - 

@article{
author = "Milović, Sanja and Stanković, Ivan and Nikolić, Dejan and Radović, Jelena and Kolundžić, Marina and Nikolić, Vesna and Stanojković, Tatjana and Petović, Slavica and Kundaković-Vasović, Tatjana",
year = "2019",
abstract = "Three seaweeds (Halimeda tuna, Codium bursa and Cystoseira barbata) and one seagrass (Cymodocea nodosa) were collected from the Coast of Montenegro, Gulf of Boka Kotorska and their chemical analysis was performed. In seagrass C. nodosa, three phenolic compounds were identified (diosmetin 7-sulfate, caftaric and coutaric acid). The content of β-glucan, fatty acids, sterols and micro- and macro-elements were investigated among all samples. The highest content of β-glucan was detected in C. nodosa seagrass (13.04±0.42 g/100 g). The highest polyunsaturated fatty acids (PUFAs) level was reported in C. barbata, the brown alga (7.157 mg/g), which also had the significant sterol content (fucosterol, 21.76±0.1 μg/g). Green algae, C. bursa and H. tuna, showed the highest level of sterols (β-sitosterol, 95.21±0.16 μg/g and 73.90±0.08 μg/g, respectively). H. tuna had the highest content of calcium (Ca) in amount of 55125 μg/g. In C. bursa, C. barbata and C. nodosa, the Na/K ratio was low (0.43, 0.46 and 0.69, respectively).",
publisher = "Wiley",
journal = "Chemistry and Biodiversity",
title = "Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro",
volume = "16",
number = "10",
pages = "1-11",
doi = "10.1002/cbdv.201900327"
}

Milović, S., Stanković, I., Nikolić, D., Radović, J., Kolundžić, M., Nikolić, V., Stanojković, T., Petović, S.,& Kundaković-Vasović, T.. (2019). Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro. in Chemistry and Biodiversity
Wiley., 16(10), 1-11.
https://doi.org/10.1002/cbdv.201900327

Milović S, Stanković I, Nikolić D, Radović J, Kolundžić M, Nikolić V, Stanojković T, Petović S, Kundaković-Vasović T. Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro. in Chemistry and Biodiversity. 2019;16(10):1-11.
doi:10.1002/cbdv.201900327 .

Milović, Sanja, Stanković, Ivan, Nikolić, Dejan, Radović, Jelena, Kolundžić, Marina, Nikolić, Vesna, Stanojković, Tatjana, Petović, Slavica, Kundaković-Vasović, Tatjana, "Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro" in Chemistry and Biodiversity, 16, no. 10 (2019):1-11,
https://doi.org/10.1002/cbdv.201900327 . .

TY  - JOUR
AU  - Milović, Sanja
AU  - Stanković, Ivan
AU  - Nikolić, Dejan
AU  - Radović, Jelena
AU  - Kolundžić, Marina
AU  - Nikolić, Vesna
AU  - Stanojković, Tatjana
AU  - Petović, Slavica
AU  - Kundaković-Vasović, Tatjana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3463
AB  - Three seaweeds (Halimeda tuna, Codium bursa and Cystoseira barbata) and one seagrass (Cymodocea nodosa) were collected from the Coast of Montenegro, Gulf of Boka Kotorska and their chemical analysis was performed. In seagrass C. nodosa, three phenolic compounds were identified (diosmetin 7-sulfate, caftaric and coutaric acid). The content of β-glucan, fatty acids, sterols and micro- and macro-elements were investigated among all samples. The highest content of β-glucan was detected in C. nodosa seagrass (13.04±0.42 g/100 g). The highest polyunsaturated fatty acids (PUFAs) level was reported in C. barbata, the brown alga (7.157 mg/g), which also had the significant sterol content (fucosterol, 21.76±0.1 μg/g). Green algae, C. bursa and H. tuna, showed the highest level of sterols (β-sitosterol, 95.21±0.16 μg/g and 73.90±0.08 μg/g, respectively). H. tuna had the highest content of calcium (Ca) in amount of 55125 μg/g. In C. bursa, C. barbata and C. nodosa, the Na/K ratio was low (0.43, 0.46 and 0.69, respectively).
PB  - Wiley
T2  - Chemistry and Biodiversity
T1  - Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro
VL  - 16
IS  - 10
SP  - 1
EP  - 11
DO  - 10.1002/cbdv.201900327
ER  - 

@article{
author = "Milović, Sanja and Stanković, Ivan and Nikolić, Dejan and Radović, Jelena and Kolundžić, Marina and Nikolić, Vesna and Stanojković, Tatjana and Petović, Slavica and Kundaković-Vasović, Tatjana",
year = "2019",
abstract = "Three seaweeds (Halimeda tuna, Codium bursa and Cystoseira barbata) and one seagrass (Cymodocea nodosa) were collected from the Coast of Montenegro, Gulf of Boka Kotorska and their chemical analysis was performed. In seagrass C. nodosa, three phenolic compounds were identified (diosmetin 7-sulfate, caftaric and coutaric acid). The content of β-glucan, fatty acids, sterols and micro- and macro-elements were investigated among all samples. The highest content of β-glucan was detected in C. nodosa seagrass (13.04±0.42 g/100 g). The highest polyunsaturated fatty acids (PUFAs) level was reported in C. barbata, the brown alga (7.157 mg/g), which also had the significant sterol content (fucosterol, 21.76±0.1 μg/g). Green algae, C. bursa and H. tuna, showed the highest level of sterols (β-sitosterol, 95.21±0.16 μg/g and 73.90±0.08 μg/g, respectively). H. tuna had the highest content of calcium (Ca) in amount of 55125 μg/g. In C. bursa, C. barbata and C. nodosa, the Na/K ratio was low (0.43, 0.46 and 0.69, respectively).",
publisher = "Wiley",
journal = "Chemistry and Biodiversity",
title = "Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro",
volume = "16",
number = "10",
pages = "1-11",
doi = "10.1002/cbdv.201900327"
}

Milović, S., Stanković, I., Nikolić, D., Radović, J., Kolundžić, M., Nikolić, V., Stanojković, T., Petović, S.,& Kundaković-Vasović, T.. (2019). Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro. in Chemistry and Biodiversity
Wiley., 16(10), 1-11.
https://doi.org/10.1002/cbdv.201900327

Milović S, Stanković I, Nikolić D, Radović J, Kolundžić M, Nikolić V, Stanojković T, Petović S, Kundaković-Vasović T. Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro. in Chemistry and Biodiversity. 2019;16(10):1-11.
doi:10.1002/cbdv.201900327 .

Milović, Sanja, Stanković, Ivan, Nikolić, Dejan, Radović, Jelena, Kolundžić, Marina, Nikolić, Vesna, Stanojković, Tatjana, Petović, Slavica, Kundaković-Vasović, Tatjana, "Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro" in Chemistry and Biodiversity, 16, no. 10 (2019):1-11,
https://doi.org/10.1002/cbdv.201900327 . .

TY  - CONF
AU  - Radović, Jelena
AU  - Milenković, Marina
AU  - Kundaković-Vasović, Tatjana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5280
AB  - Rod Alchemilla L. (Rosaceae) čine višegodišnje zeljaste biljke koje rastu na
vlažnim livadama širom Evrope, Azije i Severne Amerike. Do sada, najdetaljnije
istražena vrsta je virak, A. vulgaris L., čija se herba na našim prostorima tradicionalno
koristi za lečenje digestivnih i ginekoloških problema. Ova droga sadrži značajnu
količinu tanina, i stoga poseduje adstrigentno, antioksidativno, antimutageno dejstvo i
ostvaruje pozitivan efekat na zarastanje rana dokazan in vitro. Međutim, ostale vrste
ovog roda su znatno manje ispitane. Stoga, cilj ovog rada je ispitivanje antimikrobne i
antioksidativne aktivnost metanolnog ekstrakta vrste A. viridiflora Rothm. koja dosada
nije istražena.
Nadzemni delovi A. viridiflora prikupljeni su u julu 2013. godine na Suvoj Planini
(Srbija), nakon čega su osušeni prirodnim putem (320 g), ekstrahovani 70%
metanolom, pri čemu je dobijen metanolni ektrakt (80 g). Sadržaj ukupnih polifenola u
metanolnom ekstraktu određen je spektrofotometrijski Folin‐Ciocalteu metodom.
Sposobnost uklanjanja slobodnih radikala metanolnog ekstrakta je ispitivana
standardnim DPPH testom, i izražena kao koncentracija koja dovodi do uklanjanja 50%
DPPH radikala (IC50) i poređena sa standardnim supstancama. Antimikrobna aktivnost
metanolnog ektrakta određena je bujon dilucionom metodom na devet bakterijskih
sojeva.
Utvrđeni sadržaj ukupnih polifenola u metanolnom ekstraktu iznosi 205,8 μg
galne kiseline/g suvog ektrakta, dok je dobijena IC50 vrednost u DPPH testu bila 7,8
μg/ml. Vrednosti IC50 za vitamin C iznosi 3,8 μg/ml i za rutin 5,75 μg/ml. Metanolni
ekstrakt inhibira rast svih testiranih bakterijskih sojeva pri minimalnoj inhibitornoj
koncentraciji (MIC) od 125 μg/ml, osim bakterijskog soja Salmonella abony (MIC 62,2
μg/ml).
Metanolni ekstrakt vrste A. viridiflora ispoljava snažan antioksidativni potencijal,
približan standardnim antioksidansima, koji je u korelaciji sa visokim sadržajem
ukupnih polifenola u ekstraktu. Međutim, ispitivani ekstrakt pokazuje umerenu
antimikrobnu aktivnost na testirane bakterijske sojeve.
AB  - Plants from genus Alchemilla L. (Rosaceae) are a perennial herbaceous that grow
throughout Europe, Asia and North America. The most thoroughly studied species is the
A. vulgaris L., whose herb is used traditionally for digestive and gynecological problems.
This drug contains tannins, and therefore possesses an astringent, antioxidant,
antimutagenic effect and produces a positive effect on wound healing. However, other
species of this genus are less investigated. Therefore, the aim of this study is to
investigate the antimicrobial and antioxidant potential of the methanol extract of A.
viridiflora Rothm. which has not been studied so far.
Areal parts of A. viridiflora were collected in July 2013 at Mt. Suva Planina, dried
naturally, extracted with 70% methanol, where methanol extract was obtained. The
content of total polyphenols was determined by spectrophotometric Folin‐Ciocalteu
method. The ability of the extract to remove free radicals was tested by standard DPPH
test, and expressed as a concentration that removes 50% of DPPH radical (IC50) and
compared to standard substances. The antimicrobial activity was determined by the
broth microdilution method on nine bacterial strains.
The determined content of the total polyphenolic compounds in the methanol
extract was 205.8 μg of gallic acid/g of dry extract, while obtained IC50 in DPPH test was
7.8 μg/mL. IC50 values for vitamin C were 3.8 μg/ml and for rutin 5.75 μg/mL. The
extract inhibits the growth of all tested bacterial strains with minimum inhibitory
concentration (MIC) of 125 μg/mL, apart from the bacterial strain of Salmonella abony
(MIC of 62.2 μg/mL).
Methanol extract of A. viridiflora exhibits a strong antioxidant potential,
comparable with standard antioxidants, which was correlated with the high content of
polyphenols in the extract. However, extract shows a mild antimicrobial activity against
tested bacterial strains.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Antimikrobna i antioksidativna aktivnost metanolnog ekstrakta Alchemilla viridiflora Rothm. (Rosaceae)
T1  - Antimicrobial and antioxidant activity of methanol extract of Alchemilla viridiflora Rothm. (Rosaceae)
VL  - 68
IS  - 3
SP  - 710
EP  - 711
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5280
ER  - 

@conference{
author = "Radović, Jelena and Milenković, Marina and Kundaković-Vasović, Tatjana",
year = "2018",
abstract = "Rod Alchemilla L. (Rosaceae) čine višegodišnje zeljaste biljke koje rastu na
vlažnim livadama širom Evrope, Azije i Severne Amerike. Do sada, najdetaljnije
istražena vrsta je virak, A. vulgaris L., čija se herba na našim prostorima tradicionalno
koristi za lečenje digestivnih i ginekoloških problema. Ova droga sadrži značajnu
količinu tanina, i stoga poseduje adstrigentno, antioksidativno, antimutageno dejstvo i
ostvaruje pozitivan efekat na zarastanje rana dokazan in vitro. Međutim, ostale vrste
ovog roda su znatno manje ispitane. Stoga, cilj ovog rada je ispitivanje antimikrobne i
antioksidativne aktivnost metanolnog ekstrakta vrste A. viridiflora Rothm. koja dosada
nije istražena.
Nadzemni delovi A. viridiflora prikupljeni su u julu 2013. godine na Suvoj Planini
(Srbija), nakon čega su osušeni prirodnim putem (320 g), ekstrahovani 70%
metanolom, pri čemu je dobijen metanolni ektrakt (80 g). Sadržaj ukupnih polifenola u
metanolnom ekstraktu određen je spektrofotometrijski Folin‐Ciocalteu metodom.
Sposobnost uklanjanja slobodnih radikala metanolnog ekstrakta je ispitivana
standardnim DPPH testom, i izražena kao koncentracija koja dovodi do uklanjanja 50%
DPPH radikala (IC50) i poređena sa standardnim supstancama. Antimikrobna aktivnost
metanolnog ektrakta određena je bujon dilucionom metodom na devet bakterijskih
sojeva.
Utvrđeni sadržaj ukupnih polifenola u metanolnom ekstraktu iznosi 205,8 μg
galne kiseline/g suvog ektrakta, dok je dobijena IC50 vrednost u DPPH testu bila 7,8
μg/ml. Vrednosti IC50 za vitamin C iznosi 3,8 μg/ml i za rutin 5,75 μg/ml. Metanolni
ekstrakt inhibira rast svih testiranih bakterijskih sojeva pri minimalnoj inhibitornoj
koncentraciji (MIC) od 125 μg/ml, osim bakterijskog soja Salmonella abony (MIC 62,2
μg/ml).
Metanolni ekstrakt vrste A. viridiflora ispoljava snažan antioksidativni potencijal,
približan standardnim antioksidansima, koji je u korelaciji sa visokim sadržajem
ukupnih polifenola u ekstraktu. Međutim, ispitivani ekstrakt pokazuje umerenu
antimikrobnu aktivnost na testirane bakterijske sojeve., Plants from genus Alchemilla L. (Rosaceae) are a perennial herbaceous that grow
throughout Europe, Asia and North America. The most thoroughly studied species is the
A. vulgaris L., whose herb is used traditionally for digestive and gynecological problems.
This drug contains tannins, and therefore possesses an astringent, antioxidant,
antimutagenic effect and produces a positive effect on wound healing. However, other
species of this genus are less investigated. Therefore, the aim of this study is to
investigate the antimicrobial and antioxidant potential of the methanol extract of A.
viridiflora Rothm. which has not been studied so far.
Areal parts of A. viridiflora were collected in July 2013 at Mt. Suva Planina, dried
naturally, extracted with 70% methanol, where methanol extract was obtained. The
content of total polyphenols was determined by spectrophotometric Folin‐Ciocalteu
method. The ability of the extract to remove free radicals was tested by standard DPPH
test, and expressed as a concentration that removes 50% of DPPH radical (IC50) and
compared to standard substances. The antimicrobial activity was determined by the
broth microdilution method on nine bacterial strains.
The determined content of the total polyphenolic compounds in the methanol
extract was 205.8 μg of gallic acid/g of dry extract, while obtained IC50 in DPPH test was
7.8 μg/mL. IC50 values for vitamin C were 3.8 μg/ml and for rutin 5.75 μg/mL. The
extract inhibits the growth of all tested bacterial strains with minimum inhibitory
concentration (MIC) of 125 μg/mL, apart from the bacterial strain of Salmonella abony
(MIC of 62.2 μg/mL).
Methanol extract of A. viridiflora exhibits a strong antioxidant potential,
comparable with standard antioxidants, which was correlated with the high content of
polyphenols in the extract. However, extract shows a mild antimicrobial activity against
tested bacterial strains.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Antimikrobna i antioksidativna aktivnost metanolnog ekstrakta Alchemilla viridiflora Rothm. (Rosaceae), Antimicrobial and antioxidant activity of methanol extract of Alchemilla viridiflora Rothm. (Rosaceae)",
volume = "68",
number = "3",
pages = "710-711",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5280"
}

Radović, J., Milenković, M.,& Kundaković-Vasović, T.. (2018). Antimikrobna i antioksidativna aktivnost metanolnog ekstrakta Alchemilla viridiflora Rothm. (Rosaceae). in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 68(3), 710-711.
https://hdl.handle.net/21.15107/rcub_farfar_5280

Radović J, Milenković M, Kundaković-Vasović T. Antimikrobna i antioksidativna aktivnost metanolnog ekstrakta Alchemilla viridiflora Rothm. (Rosaceae). in Arhiv za farmaciju. 2018;68(3):710-711.
https://hdl.handle.net/21.15107/rcub_farfar_5280 .

Radović, Jelena, Milenković, Marina, Kundaković-Vasović, Tatjana, "Antimikrobna i antioksidativna aktivnost metanolnog ekstrakta Alchemilla viridiflora Rothm. (Rosaceae)" in Arhiv za farmaciju, 68, no. 3 (2018):710-711,
https://hdl.handle.net/21.15107/rcub_farfar_5280 .

TY  - CONF
AU  - Radović, Jelena
AU  - Grozdanić, Nađa
AU  - Stanojković, Tatjana
AU  - Suručić, Relja
AU  - Kundaković-Vasović, Tatjana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5277
AB  - Tanini, polifenolni biljni metaboliti, značajno smanjuju postprandijalnu
hiperglikemiju inhibicijom α‐glukozidaze, i stoga mogu biti efikasna strategija u
kontroli dijabetesa tipa 2. Takođe, dokazano je i da nespecifično inhibiraju aktivnost
angiotenzin‐konvertujućeg enzima (ACE). Kako su tanini identifikovani samo u vrsti
Alchemilla vulgaris L., cilj ovog istraživanja je da se odredi sadržaj tanina u do sada
neistraženoj vrsti A. viridiflora Rothm. (Rosaceae), kao i inhibitorni uticaj na aktivnost
ACE i α‐glukozidaze.
Ukupni sadržaj tanina u metanolnom ekstraktu A. viridiflora određen je prema
propisu Ph. Eur. 9.0. Suvi metanolni ekstrakt, enzimski rastvor (400 mU/ml α‐
glukozidaze u 0,1 M fosfatnom puferu) i supstrat, p‐nitrofenil α‐D‐glukopiranozid
korišćeni su za kolorimetrijski test inhibitorne aktivnosti α‐glukozidaze. Kao pozitivna
kontrola korišćena je akarboza. ACE inhibitorna aktivnost metanolnog ekstrakta
ispitana je korišćenjem komercijalnog testa ACE Kit‐ WST (Dojindo Inc., Japan) prema
uputstvu proizvođača. Procenat inhibicije enzima je izračunata IC50 vrednost, tj.
procenjena koncentracija ekstrakta koja je izazvala 50% inhibicije aktivnosti enzima,
koristeći linearnu regresionu analizu.
IC50 vrednost metanolnog ekstrakta A. viridiflora, očitana sa dozno‐zavisne krive
iznosi 2,6±0,5 μg/ml, i ekstrakt pokazuje bolju anti‐α‐glukozidaznu aktivnost od
standarda akarboze (IC50=74,2±3,3 μg/ml). Takođe, ispitivani ekstrakt pokazuje doznozavisnu
inhibiciju ACE pri IC50 2 μg/ml. Dobijeni rezultati su u korelaciji sa visokim
sadržajem tanina u metanolnom ekstraktu A. viridiflora (3,74 %).
Pokazane inhibicije angiotenzin‐konvertujućeg enzima i α‐glukozidaze čine
metanolni ekstrakt vrste A. viridiflora pogodnim za dalje istraživanje u cilju
pronalaženja novih prirodnih proizvoda značajnih za terapiju kardiovaskularnih bolesti
i dijabetesa.
AB  - Tannins, polyphenolic plant metabolites, significantly reduce postprandial
hyperglycemia by inhibiting α‐glucosidase, and therefore can be an effective strategy
for controlling type 2 diabetes. It has also been proven that they are non‐specific
inhibitors of the activity of angiotensin‐converting enzyme (ACE). As tannins were
identified only in the species Alchemilla vulgaris L., the aim of this study is to determine
the content of tannins in the unexplored A. viridiflora Rothm. (Rosaceae), as well as the
inhibitory effect on the activity of angiotensin‐converting enzyme and α‐glucosidase.
The content of tannins in methanol extract of A. viridiflora was determined
according to the Ph. Eur. 9.0. Dry methanol extract, enzyme solution (400 mU/ml of α‐
glucosidase in 0.1 M phosphate buffer) and substrate, p‐nitrophenyl α‐Dglucopyranoside
were used for colorimetric α‐glucosidase inhibitory activity test.
Acarbose was used as a positive control. The ACE inhibitory activity of the methanol
extract was tested using the commercial ACE Kit‐WST (Dojindo Inc., Japan) according to
the manufacturer's instructions. The percentage of enzyme inhibition is the calculated
IC50 value, i.e. estimated concentration of the extract that caused 50% inhibition of
enzyme activity using linear regression analysis.
The IC50 of A. viridiflora methanol extract, read from the dose‐dependent curve,
was 2.6±0.5 μg/mL, and this extract demonstrated better anti‐α‐glucosidase activity
than standard acarbose (IC50=74.2±3.3 μg/mL). In addition, the examined extract
shows a dose‐dependent inhibition of ACE with IC50 2 μg/mL. Obtained results were in
correlation with high level of tannins in methanol extract of A. viridiflora (3.74%).
The proven inhibitions of ACE and α-glucosidase make the methanol extract
of A. viridiflora suitable for further scientific research in order to find a new natural
product for the treatment of cardiovascular diseases and diabetes.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - ACE i α‐glukozidazna inhibitorna aktivnost metanolnog ekstrakta Alchemilla viridiflora Rothm. (Rosaceae)
T1  - ACE and α‐glucosidase inhibitory activity of methanol extract of Alchemilla viridiflora Rothm. (Rosaceae)
VL  - 68
IS  - 2
SP  - 342
EP  - 343
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5277
ER  - 

@conference{
author = "Radović, Jelena and Grozdanić, Nađa and Stanojković, Tatjana and Suručić, Relja and Kundaković-Vasović, Tatjana",
year = "2018",
abstract = "Tanini, polifenolni biljni metaboliti, značajno smanjuju postprandijalnu
hiperglikemiju inhibicijom α‐glukozidaze, i stoga mogu biti efikasna strategija u
kontroli dijabetesa tipa 2. Takođe, dokazano je i da nespecifično inhibiraju aktivnost
angiotenzin‐konvertujućeg enzima (ACE). Kako su tanini identifikovani samo u vrsti
Alchemilla vulgaris L., cilj ovog istraživanja je da se odredi sadržaj tanina u do sada
neistraženoj vrsti A. viridiflora Rothm. (Rosaceae), kao i inhibitorni uticaj na aktivnost
ACE i α‐glukozidaze.
Ukupni sadržaj tanina u metanolnom ekstraktu A. viridiflora određen je prema
propisu Ph. Eur. 9.0. Suvi metanolni ekstrakt, enzimski rastvor (400 mU/ml α‐
glukozidaze u 0,1 M fosfatnom puferu) i supstrat, p‐nitrofenil α‐D‐glukopiranozid
korišćeni su za kolorimetrijski test inhibitorne aktivnosti α‐glukozidaze. Kao pozitivna
kontrola korišćena je akarboza. ACE inhibitorna aktivnost metanolnog ekstrakta
ispitana je korišćenjem komercijalnog testa ACE Kit‐ WST (Dojindo Inc., Japan) prema
uputstvu proizvođača. Procenat inhibicije enzima je izračunata IC50 vrednost, tj.
procenjena koncentracija ekstrakta koja je izazvala 50% inhibicije aktivnosti enzima,
koristeći linearnu regresionu analizu.
IC50 vrednost metanolnog ekstrakta A. viridiflora, očitana sa dozno‐zavisne krive
iznosi 2,6±0,5 μg/ml, i ekstrakt pokazuje bolju anti‐α‐glukozidaznu aktivnost od
standarda akarboze (IC50=74,2±3,3 μg/ml). Takođe, ispitivani ekstrakt pokazuje doznozavisnu
inhibiciju ACE pri IC50 2 μg/ml. Dobijeni rezultati su u korelaciji sa visokim
sadržajem tanina u metanolnom ekstraktu A. viridiflora (3,74 %).
Pokazane inhibicije angiotenzin‐konvertujućeg enzima i α‐glukozidaze čine
metanolni ekstrakt vrste A. viridiflora pogodnim za dalje istraživanje u cilju
pronalaženja novih prirodnih proizvoda značajnih za terapiju kardiovaskularnih bolesti
i dijabetesa., Tannins, polyphenolic plant metabolites, significantly reduce postprandial
hyperglycemia by inhibiting α‐glucosidase, and therefore can be an effective strategy
for controlling type 2 diabetes. It has also been proven that they are non‐specific
inhibitors of the activity of angiotensin‐converting enzyme (ACE). As tannins were
identified only in the species Alchemilla vulgaris L., the aim of this study is to determine
the content of tannins in the unexplored A. viridiflora Rothm. (Rosaceae), as well as the
inhibitory effect on the activity of angiotensin‐converting enzyme and α‐glucosidase.
The content of tannins in methanol extract of A. viridiflora was determined
according to the Ph. Eur. 9.0. Dry methanol extract, enzyme solution (400 mU/ml of α‐
glucosidase in 0.1 M phosphate buffer) and substrate, p‐nitrophenyl α‐Dglucopyranoside
were used for colorimetric α‐glucosidase inhibitory activity test.
Acarbose was used as a positive control. The ACE inhibitory activity of the methanol
extract was tested using the commercial ACE Kit‐WST (Dojindo Inc., Japan) according to
the manufacturer's instructions. The percentage of enzyme inhibition is the calculated
IC50 value, i.e. estimated concentration of the extract that caused 50% inhibition of
enzyme activity using linear regression analysis.
The IC50 of A. viridiflora methanol extract, read from the dose‐dependent curve,
was 2.6±0.5 μg/mL, and this extract demonstrated better anti‐α‐glucosidase activity
than standard acarbose (IC50=74.2±3.3 μg/mL). In addition, the examined extract
shows a dose‐dependent inhibition of ACE with IC50 2 μg/mL. Obtained results were in
correlation with high level of tannins in methanol extract of A. viridiflora (3.74%).
The proven inhibitions of ACE and α-glucosidase make the methanol extract
of A. viridiflora suitable for further scientific research in order to find a new natural
product for the treatment of cardiovascular diseases and diabetes.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "ACE i α‐glukozidazna inhibitorna aktivnost metanolnog ekstrakta Alchemilla viridiflora Rothm. (Rosaceae), ACE and α‐glucosidase inhibitory activity of methanol extract of Alchemilla viridiflora Rothm. (Rosaceae)",
volume = "68",
number = "2",
pages = "342-343",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5277"
}

Radović, J., Grozdanić, N., Stanojković, T., Suručić, R.,& Kundaković-Vasović, T.. (2018). ACE i α‐glukozidazna inhibitorna aktivnost metanolnog ekstrakta Alchemilla viridiflora Rothm. (Rosaceae). in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 68(2), 342-343.
https://hdl.handle.net/21.15107/rcub_farfar_5277

Radović J, Grozdanić N, Stanojković T, Suručić R, Kundaković-Vasović T. ACE i α‐glukozidazna inhibitorna aktivnost metanolnog ekstrakta Alchemilla viridiflora Rothm. (Rosaceae). in Arhiv za farmaciju. 2018;68(2):342-343.
https://hdl.handle.net/21.15107/rcub_farfar_5277 .

Radović, Jelena, Grozdanić, Nađa, Stanojković, Tatjana, Suručić, Relja, Kundaković-Vasović, Tatjana, "ACE i α‐glukozidazna inhibitorna aktivnost metanolnog ekstrakta Alchemilla viridiflora Rothm. (Rosaceae)" in Arhiv za farmaciju, 68, no. 2 (2018):342-343,
https://hdl.handle.net/21.15107/rcub_farfar_5277 .

TY  - CONF
AU  - Kała, Katarzyna
AU  - Krakowska, Agata
AU  - Radović, Jelena
AU  - Kundaković, Tatjana
AU  - Muszyńska, Bożena
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5274
AB  - Tricholoma equestre (L.) P. Kumm. (Man on horseback) is a popular species
of edible mushroom. This species was selected for the experiment because it is a
commonly consumed mushroom that is allowed to be sold on bazaar in many
countries include Poland. It was proven that this species is a good source of zinc and
other biologically active substances. Based on scientific data is good known that
biologically active constituents of Tricholoma equestre − fatty acids, exhibit role in
prophylaxis of hypertension and coronary heart disease.
Thereby, the aim of the research was to study content of fatty acids and their
profile in lyophilized fruiting bodies of Tricholoma equestre. In order to study the
chemical composition of fatty acids, their cyclohexane extract was studied using GCMS.
Fatty acids were esterified using 98% H2SO4/MeOH anh. to obtain FAMEs (fatty
acid methyl esters).
Eleven fatty acids were detected and quantified: myristic acid, pentadecanoic
acid, palmitic acid, heptadecanoic acid, stearic acid, oleic acid, vaccenic acid, linoleic
acid, arachidonic acid, behenic acid, and lignoceric acid. Palmitic, oleic and linoleic
acids were determined in highest amounts (respectively 28.84%, 28.59%, and 28.29%
of total content). The analyzed total fatty acids profile contain saturated fatty acids
(40.25%), monounsaturated fatty acids (31.12%) and polyunsaturated fatty acids
(28.63%).
To ensure the proper functioning of the human body, it is necessary to have a
balance between the saturated and unsaturated fatty acids in daily diet. Based on
obtained results Tricholoma equestre species is a good natural source of unsaturated
fatty acids and can be a valuable component of everyday diet.
PB  - Faculty of Chemical and Process Engineering, Warsaw University of Technology
C3  - EYEC Monograph, 7th European Young Engineers Conference
T1  - Analysis of fatty acids composition in Tricholoma equestre fruiting bodies
SP  - 503
EP  - 503
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5274
ER  - 

@conference{
author = "Kała, Katarzyna and Krakowska, Agata and Radović, Jelena and Kundaković, Tatjana and Muszyńska, Bożena",
year = "2018",
abstract = "Tricholoma equestre (L.) P. Kumm. (Man on horseback) is a popular species
of edible mushroom. This species was selected for the experiment because it is a
commonly consumed mushroom that is allowed to be sold on bazaar in many
countries include Poland. It was proven that this species is a good source of zinc and
other biologically active substances. Based on scientific data is good known that
biologically active constituents of Tricholoma equestre − fatty acids, exhibit role in
prophylaxis of hypertension and coronary heart disease.
Thereby, the aim of the research was to study content of fatty acids and their
profile in lyophilized fruiting bodies of Tricholoma equestre. In order to study the
chemical composition of fatty acids, their cyclohexane extract was studied using GCMS.
Fatty acids were esterified using 98% H2SO4/MeOH anh. to obtain FAMEs (fatty
acid methyl esters).
Eleven fatty acids were detected and quantified: myristic acid, pentadecanoic
acid, palmitic acid, heptadecanoic acid, stearic acid, oleic acid, vaccenic acid, linoleic
acid, arachidonic acid, behenic acid, and lignoceric acid. Palmitic, oleic and linoleic
acids were determined in highest amounts (respectively 28.84%, 28.59%, and 28.29%
of total content). The analyzed total fatty acids profile contain saturated fatty acids
(40.25%), monounsaturated fatty acids (31.12%) and polyunsaturated fatty acids
(28.63%).
To ensure the proper functioning of the human body, it is necessary to have a
balance between the saturated and unsaturated fatty acids in daily diet. Based on
obtained results Tricholoma equestre species is a good natural source of unsaturated
fatty acids and can be a valuable component of everyday diet.",
publisher = "Faculty of Chemical and Process Engineering, Warsaw University of Technology",
journal = "EYEC Monograph, 7th European Young Engineers Conference",
title = "Analysis of fatty acids composition in Tricholoma equestre fruiting bodies",
pages = "503-503",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5274"
}

Kała, K., Krakowska, A., Radović, J., Kundaković, T.,& Muszyńska, B.. (2018). Analysis of fatty acids composition in Tricholoma equestre fruiting bodies. in EYEC Monograph, 7th European Young Engineers Conference
Faculty of Chemical and Process Engineering, Warsaw University of Technology., 503-503.
https://hdl.handle.net/21.15107/rcub_farfar_5274

Kała K, Krakowska A, Radović J, Kundaković T, Muszyńska B. Analysis of fatty acids composition in Tricholoma equestre fruiting bodies. in EYEC Monograph, 7th European Young Engineers Conference. 2018;:503-503.
https://hdl.handle.net/21.15107/rcub_farfar_5274 .

Kała, Katarzyna, Krakowska, Agata, Radović, Jelena, Kundaković, Tatjana, Muszyńska, Bożena, "Analysis of fatty acids composition in Tricholoma equestre fruiting bodies" in EYEC Monograph, 7th European Young Engineers Conference (2018):503-503,
https://hdl.handle.net/21.15107/rcub_farfar_5274 .

TY  - CONF
AU  - Radović, Jelena
AU  - Perišić, Ivana
AU  - Kundaković, Tatjana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5273
AB  - Tea tree oil, the essential oil derived mainly from the Australian native plant Melaleuca alternifolia L. (Myrtaceae) is widely used as the active ingredient in many topical formulations for its antimicrobial activity, acting against viruses, bacteria, and fungi. Clinical studies have shown that this essential oil is very effective in various skin conditions, including acne, but also as an antiseptic in dentistry [1,2]. Since tea tree oil is commonly used, the aim of this study was to investigate the composition and quality of the three bestselling commercial tea tree oils in the Republic of Serbia, using GC and GC-MS analyses. All three oils had an almost identical composition. Among 42 quantified volatile substances, the most abundant in all samples were the oxygenated monoterpenes (46%), monoterpene hydrocarbons (45%), sesquiterpene hydrocarbons (6%), and oxygenated sesquiterpenes (1.5%). The major constituents are terpinen-4-ol (39.2%), γ-terpinene (17.6%), α-terpinene (7.4%), p-cymene (6.4%), limonene (4.3%), 1,8-cineol (3.7%), terpinolene (3.5%), α-pinene (3.2%), α-terpineol (2.8%), aromadendrene (0.9%), and sabinene (0.2%). The contents of all major components were within the range required by Ph. Eur. 9.0. In addition, all samples were tested for their quality based on measuring specific physical-chemical parameters (relative density, refractive index, and optical rotation) using suitable methods according to Ph. Eur. 9.0. Thus, it can be concluded that the tested samples of tea tree oil available on our market are of adequate pharmacopoeial quality.
PB  - University of Niš, Serbia
C3  - Facta Universitatis, Series: Physics, Chemistry and Technology: 49th International Symposium on Essential Oils (ISEO2018), Book of Abstracts
T1  - The analyses of commercial tea tree oils
VL  - 16
IS  - 1 Special issue
SP  - 104
EP  - 104
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5273
ER  - 

@conference{
author = "Radović, Jelena and Perišić, Ivana and Kundaković, Tatjana",
year = "2018",
abstract = "Tea tree oil, the essential oil derived mainly from the Australian native plant Melaleuca alternifolia L. (Myrtaceae) is widely used as the active ingredient in many topical formulations for its antimicrobial activity, acting against viruses, bacteria, and fungi. Clinical studies have shown that this essential oil is very effective in various skin conditions, including acne, but also as an antiseptic in dentistry [1,2]. Since tea tree oil is commonly used, the aim of this study was to investigate the composition and quality of the three bestselling commercial tea tree oils in the Republic of Serbia, using GC and GC-MS analyses. All three oils had an almost identical composition. Among 42 quantified volatile substances, the most abundant in all samples were the oxygenated monoterpenes (46%), monoterpene hydrocarbons (45%), sesquiterpene hydrocarbons (6%), and oxygenated sesquiterpenes (1.5%). The major constituents are terpinen-4-ol (39.2%), γ-terpinene (17.6%), α-terpinene (7.4%), p-cymene (6.4%), limonene (4.3%), 1,8-cineol (3.7%), terpinolene (3.5%), α-pinene (3.2%), α-terpineol (2.8%), aromadendrene (0.9%), and sabinene (0.2%). The contents of all major components were within the range required by Ph. Eur. 9.0. In addition, all samples were tested for their quality based on measuring specific physical-chemical parameters (relative density, refractive index, and optical rotation) using suitable methods according to Ph. Eur. 9.0. Thus, it can be concluded that the tested samples of tea tree oil available on our market are of adequate pharmacopoeial quality.",
publisher = "University of Niš, Serbia",
journal = "Facta Universitatis, Series: Physics, Chemistry and Technology: 49th International Symposium on Essential Oils (ISEO2018), Book of Abstracts",
title = "The analyses of commercial tea tree oils",
volume = "16",
number = "1 Special issue",
pages = "104-104",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5273"
}

Radović, J., Perišić, I.,& Kundaković, T.. (2018). The analyses of commercial tea tree oils. in Facta Universitatis, Series: Physics, Chemistry and Technology: 49th International Symposium on Essential Oils (ISEO2018), Book of Abstracts
University of Niš, Serbia., 16(1 Special issue), 104-104.
https://hdl.handle.net/21.15107/rcub_farfar_5273

Radović J, Perišić I, Kundaković T. The analyses of commercial tea tree oils. in Facta Universitatis, Series: Physics, Chemistry and Technology: 49th International Symposium on Essential Oils (ISEO2018), Book of Abstracts. 2018;16(1 Special issue):104-104.
https://hdl.handle.net/21.15107/rcub_farfar_5273 .

Radović, Jelena, Perišić, Ivana, Kundaković, Tatjana, "The analyses of commercial tea tree oils" in Facta Universitatis, Series: Physics, Chemistry and Technology: 49th International Symposium on Essential Oils (ISEO2018), Book of Abstracts, 16, no. 1 Special issue (2018):104-104,
https://hdl.handle.net/21.15107/rcub_farfar_5273 .

TY  - CONF
AU  - Marčetić, Mirjana
AU  - Škobić, Slađana
AU  - Radović, Jelena
AU  - Bošković, Nikola
AU  - Damjanović, Anđela
AU  - Kundaković, Tatjana
AU  - Crnobarac, Jovan
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5272
AB  - Sweet flag rhizome (Acorus calamus L., Acoraceae) possesses various biological activities such as sedative, anticonvulsant, immunosuppressant, antidiabetic, antiinflammatory etc. [1]. The aim was to study the content and composition of essential oils from 24 rhizome samples of the cultivated sweet flag, their comparison with the samples from 5 natural habitats, and the influence of nitrogen fertilization on the composition of essential oils. Essential oils were analyzed using GC and GC/MS. Statistical analysis included the analysis of variance and cluster analysis. The content of essential oil was not significantly different between the samples from natural habitats (0.8-1.1%) and cultures (0.3-2.2%). All samples contained dominant oxygenated sesquiterpenes (24.5-32.6%), phenylpropanoids (5.7-22.5%), and oxygenated monoterpenes (4.4-19.2%). The main components were β-asarone (10.4-21.4%), camphor (3.5-15.2%), acorenone (9.3-14.1%) and cyperotudone (7.3-11.0%). The contents of β-asarone and aristolone were significantly higher (p<0.05) in cultivated plants. The nitrogen fertilization during the cultivation did not have a significant influence (p<0.05) on the content and composition of the essential oils.
PB  - University of Niš, Serbia
C3  - Facta Universitatis, Series: Physics, Chemistry and Technology: 49th International Symposium on Essential Oils (ISEO2018), Book of Abstracts
T1  - The composition of the essential oils of Acorus calamus L. rhizomes from different habitats
VL  - 16
IS  - 1 Special issue
SP  - 105
EP  - 105
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5272
ER  - 

@conference{
author = "Marčetić, Mirjana and Škobić, Slađana and Radović, Jelena and Bošković, Nikola and Damjanović, Anđela and Kundaković, Tatjana and Crnobarac, Jovan",
year = "2018",
abstract = "Sweet flag rhizome (Acorus calamus L., Acoraceae) possesses various biological activities such as sedative, anticonvulsant, immunosuppressant, antidiabetic, antiinflammatory etc. [1]. The aim was to study the content and composition of essential oils from 24 rhizome samples of the cultivated sweet flag, their comparison with the samples from 5 natural habitats, and the influence of nitrogen fertilization on the composition of essential oils. Essential oils were analyzed using GC and GC/MS. Statistical analysis included the analysis of variance and cluster analysis. The content of essential oil was not significantly different between the samples from natural habitats (0.8-1.1%) and cultures (0.3-2.2%). All samples contained dominant oxygenated sesquiterpenes (24.5-32.6%), phenylpropanoids (5.7-22.5%), and oxygenated monoterpenes (4.4-19.2%). The main components were β-asarone (10.4-21.4%), camphor (3.5-15.2%), acorenone (9.3-14.1%) and cyperotudone (7.3-11.0%). The contents of β-asarone and aristolone were significantly higher (p<0.05) in cultivated plants. The nitrogen fertilization during the cultivation did not have a significant influence (p<0.05) on the content and composition of the essential oils.",
publisher = "University of Niš, Serbia",
journal = "Facta Universitatis, Series: Physics, Chemistry and Technology: 49th International Symposium on Essential Oils (ISEO2018), Book of Abstracts",
title = "The composition of the essential oils of Acorus calamus L. rhizomes from different habitats",
volume = "16",
number = "1 Special issue",
pages = "105-105",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5272"
}

Marčetić, M., Škobić, S., Radović, J., Bošković, N., Damjanović, A., Kundaković, T.,& Crnobarac, J.. (2018). The composition of the essential oils of Acorus calamus L. rhizomes from different habitats. in Facta Universitatis, Series: Physics, Chemistry and Technology: 49th International Symposium on Essential Oils (ISEO2018), Book of Abstracts
University of Niš, Serbia., 16(1 Special issue), 105-105.
https://hdl.handle.net/21.15107/rcub_farfar_5272

Marčetić M, Škobić S, Radović J, Bošković N, Damjanović A, Kundaković T, Crnobarac J. The composition of the essential oils of Acorus calamus L. rhizomes from different habitats. in Facta Universitatis, Series: Physics, Chemistry and Technology: 49th International Symposium on Essential Oils (ISEO2018), Book of Abstracts. 2018;16(1 Special issue):105-105.
https://hdl.handle.net/21.15107/rcub_farfar_5272 .

Marčetić, Mirjana, Škobić, Slađana, Radović, Jelena, Bošković, Nikola, Damjanović, Anđela, Kundaković, Tatjana, Crnobarac, Jovan, "The composition of the essential oils of Acorus calamus L. rhizomes from different habitats" in Facta Universitatis, Series: Physics, Chemistry and Technology: 49th International Symposium on Essential Oils (ISEO2018), Book of Abstracts, 16, no. 1 Special issue (2018):105-105,
https://hdl.handle.net/21.15107/rcub_farfar_5272 .

TY  - CONF
AU  - Muszyńska, Bożena
AU  - Radović, Jelena
AU  - Kała, Katarzyna
AU  - Gdula-Argasińska, Joanna
AU  - Lazur, Jan
AU  - Sułkowska-Ziaja, Katarzyna
AU  - Kundaković, Tatjana
AU  - Starek, Małgorzata
AU  - Opoka, Włodzimierz
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5221
AB  - In the presented study extract from fruiting bodies of Tricholoma equestre (Man on horseback) were used. Fruiting bodies of this species were collected from the natural environment in mixed forests of South Poland.
PB  - International Organitation for Science and Technology education (IOSTE)
C3  - IOSTE 2018 Dijon, Maison des Sciences de l’Homme, April 25-26
T1  - Pro-inflammatory activity of mushrooms species Tricholoma equestre
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5221
ER  - 

@conference{
author = "Muszyńska, Bożena and Radović, Jelena and Kała, Katarzyna and Gdula-Argasińska, Joanna and Lazur, Jan and Sułkowska-Ziaja, Katarzyna and Kundaković, Tatjana and Starek, Małgorzata and Opoka, Włodzimierz",
year = "2018",
abstract = "In the presented study extract from fruiting bodies of Tricholoma equestre (Man on horseback) were used. Fruiting bodies of this species were collected from the natural environment in mixed forests of South Poland.",
publisher = "International Organitation for Science and Technology education (IOSTE)",
journal = "IOSTE 2018 Dijon, Maison des Sciences de l’Homme, April 25-26",
title = "Pro-inflammatory activity of mushrooms species Tricholoma equestre",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5221"
}

Muszyńska, B., Radović, J., Kała, K., Gdula-Argasińska, J., Lazur, J., Sułkowska-Ziaja, K., Kundaković, T., Starek, M.,& Opoka, W.. (2018). Pro-inflammatory activity of mushrooms species Tricholoma equestre. in IOSTE 2018 Dijon, Maison des Sciences de l’Homme, April 25-26
International Organitation for Science and Technology education (IOSTE)..
https://hdl.handle.net/21.15107/rcub_farfar_5221

Muszyńska B, Radović J, Kała K, Gdula-Argasińska J, Lazur J, Sułkowska-Ziaja K, Kundaković T, Starek M, Opoka W. Pro-inflammatory activity of mushrooms species Tricholoma equestre. in IOSTE 2018 Dijon, Maison des Sciences de l’Homme, April 25-26. 2018;.
https://hdl.handle.net/21.15107/rcub_farfar_5221 .

Muszyńska, Bożena, Radović, Jelena, Kała, Katarzyna, Gdula-Argasińska, Joanna, Lazur, Jan, Sułkowska-Ziaja, Katarzyna, Kundaković, Tatjana, Starek, Małgorzata, Opoka, Włodzimierz, "Pro-inflammatory activity of mushrooms species Tricholoma equestre" in IOSTE 2018 Dijon, Maison des Sciences de l’Homme, April 25-26 (2018),
https://hdl.handle.net/21.15107/rcub_farfar_5221 .