TY  - JOUR
AU  - Zečević, Mira
AU  - Stanković, Z
AU  - Živanović, L
AU  - Jocić, B
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/815
AB  - The novel, rapid high performance liquid chromatographic method for the determination of tramadol hydrochloride and its three impurities was developed and validated. The method can simultaneously assay potassium sorbate, used as preservative. and saccharin sodium, used as sweetener in tramadol pharmaceutical formulation. The separation was carried out on a C-18 XTerra (TM) (150 mm x 4.6 mm, 5 mm) column using acetonitrile-0.015 M Na2HPO4 buffer (2:8, v/v) as mobile phase (pH value 3.0 was adjusted with orthophosphoric acid) at a flow rate 1.0 ml min(-1), temperature of the column 20 degrees C and UV detection at 218 nm. The method was found to be linear (r > 0.999) in the range of 0.05-0.8 mg ml(-1) for tramadol hydrochloride, 0.1-1.2 mg ml(-1) for impurities B and C and for impurity A (r > 0.995) in the range 0.15-2.4 mg ml(-1). The low RSD values indicate good precision and high recovery values indicate excellent accuracy of the HPLC method. Developed method was successfully applied to the determination of tramadol hydrochloride. its investigated impurities and potassium sorbate in commercial formulation. The recovery of tramadol hydrochloride was 98.25% and RSD was 1.80%. The method is rapid and sensitive enough to be used to analyse Trodon((R)) oral drops.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Chromatography A
T1  - Validation of a high-performance liquid chromatographic method for the simultaneous determination of tramadol and its impurities in oral drops as a pharmaceutical formulation
VL  - 1119
IS  - 1-2
SP  - 251
EP  - 256
DO  - 10.1016/j.chroma.2005.11.105
ER  - 

@article{
author = "Zečević, Mira and Stanković, Z and Živanović, L and Jocić, B",
year = "2006",
abstract = "The novel, rapid high performance liquid chromatographic method for the determination of tramadol hydrochloride and its three impurities was developed and validated. The method can simultaneously assay potassium sorbate, used as preservative. and saccharin sodium, used as sweetener in tramadol pharmaceutical formulation. The separation was carried out on a C-18 XTerra (TM) (150 mm x 4.6 mm, 5 mm) column using acetonitrile-0.015 M Na2HPO4 buffer (2:8, v/v) as mobile phase (pH value 3.0 was adjusted with orthophosphoric acid) at a flow rate 1.0 ml min(-1), temperature of the column 20 degrees C and UV detection at 218 nm. The method was found to be linear (r > 0.999) in the range of 0.05-0.8 mg ml(-1) for tramadol hydrochloride, 0.1-1.2 mg ml(-1) for impurities B and C and for impurity A (r > 0.995) in the range 0.15-2.4 mg ml(-1). The low RSD values indicate good precision and high recovery values indicate excellent accuracy of the HPLC method. Developed method was successfully applied to the determination of tramadol hydrochloride. its investigated impurities and potassium sorbate in commercial formulation. The recovery of tramadol hydrochloride was 98.25% and RSD was 1.80%. The method is rapid and sensitive enough to be used to analyse Trodon((R)) oral drops.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Chromatography A",
title = "Validation of a high-performance liquid chromatographic method for the simultaneous determination of tramadol and its impurities in oral drops as a pharmaceutical formulation",
volume = "1119",
number = "1-2",
pages = "251-256",
doi = "10.1016/j.chroma.2005.11.105"
}

Zečević, M., Stanković, Z., Živanović, L.,& Jocić, B.. (2006). Validation of a high-performance liquid chromatographic method for the simultaneous determination of tramadol and its impurities in oral drops as a pharmaceutical formulation. in Journal of Chromatography A
Elsevier Science BV, Amsterdam., 1119(1-2), 251-256.
https://doi.org/10.1016/j.chroma.2005.11.105

Zečević M, Stanković Z, Živanović L, Jocić B. Validation of a high-performance liquid chromatographic method for the simultaneous determination of tramadol and its impurities in oral drops as a pharmaceutical formulation. in Journal of Chromatography A. 2006;1119(1-2):251-256.
doi:10.1016/j.chroma.2005.11.105 .

Zečević, Mira, Stanković, Z, Živanović, L, Jocić, B, "Validation of a high-performance liquid chromatographic method for the simultaneous determination of tramadol and its impurities in oral drops as a pharmaceutical formulation" in Journal of Chromatography A, 1119, no. 1-2 (2006):251-256,
https://doi.org/10.1016/j.chroma.2005.11.105 . .

TY  - JOUR
AU  - Zečević, Mira
AU  - Savić, G
AU  - Živanović, L
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/775
AB  - A reversed-phase liquid chromatography method has been developed for the separation of valdecoxib and impurity SC-77852. The best results were achieved using a mobile phase-methanol: 1% water solution TEA (52 : 48 v/v), pH 7.35 (adjusted with 85% orthophosphoric acid), column temperature 24 degrees C. Separation was carried out on XTerra (TM) RP18 (150mm x 4,6 mm), particle size 5 mu m, flow rate 1ml/min, using detection on 220 nm. The method was statistically validated for its selectivity, linearity, precision (repeatability), and robustness. Quantitation and detection limits were determined for both valdecoxib and SC-77852. Method robustness was further evaluated by performing full factorial design experiment. Validated method was used for assay of valdecoxib and SC-77852 in Bextra (R) film-coated tablets.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Analytical Letters
T1  - Development and validation of liquid chromatography method for the separation of valdecoxib and its SC-77852 impurity
VL  - 39
IS  - 9
SP  - 1875
EP  - 1890
DO  - 10.1080/00032710600721605
ER  - 

@article{
author = "Zečević, Mira and Savić, G and Živanović, L",
year = "2006",
abstract = "A reversed-phase liquid chromatography method has been developed for the separation of valdecoxib and impurity SC-77852. The best results were achieved using a mobile phase-methanol: 1% water solution TEA (52 : 48 v/v), pH 7.35 (adjusted with 85% orthophosphoric acid), column temperature 24 degrees C. Separation was carried out on XTerra (TM) RP18 (150mm x 4,6 mm), particle size 5 mu m, flow rate 1ml/min, using detection on 220 nm. The method was statistically validated for its selectivity, linearity, precision (repeatability), and robustness. Quantitation and detection limits were determined for both valdecoxib and SC-77852. Method robustness was further evaluated by performing full factorial design experiment. Validated method was used for assay of valdecoxib and SC-77852 in Bextra (R) film-coated tablets.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Analytical Letters",
title = "Development and validation of liquid chromatography method for the separation of valdecoxib and its SC-77852 impurity",
volume = "39",
number = "9",
pages = "1875-1890",
doi = "10.1080/00032710600721605"
}

Zečević, M., Savić, G.,& Živanović, L.. (2006). Development and validation of liquid chromatography method for the separation of valdecoxib and its SC-77852 impurity. in Analytical Letters
Taylor & Francis Inc, Philadelphia., 39(9), 1875-1890.
https://doi.org/10.1080/00032710600721605

Zečević M, Savić G, Živanović L. Development and validation of liquid chromatography method for the separation of valdecoxib and its SC-77852 impurity. in Analytical Letters. 2006;39(9):1875-1890.
doi:10.1080/00032710600721605 .

Zečević, Mira, Savić, G, Živanović, L, "Development and validation of liquid chromatography method for the separation of valdecoxib and its SC-77852 impurity" in Analytical Letters, 39, no. 9 (2006):1875-1890,
https://doi.org/10.1080/00032710600721605 . .

TY  - JOUR
AU  - Živanović, L
AU  - Zečević, Mira
AU  - Marković, S
AU  - Petrović, S
AU  - Ivanović, I
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/573
AB  - In this paper, there was developed a sensitive, precise and accurate reversed-phase liquid chromatographic (RP-HPLC) method and validated for simultaneous determination of lidocaine hydrochloride, dexamethasone acetate (DA) and calcium dobesilate (CD) in suppositories and ointment. Also there was achieved a parallel analysis of buthylhydroxyanisol, as a preservative, and hydroquinone, as a degradation product of calcium dobesilate, present in these dosage forms. The relative standard deviation (RSD) values for all five compounds indicated a good precision and accuracy of the RP-HPLC method. Method is selective, sensitive and reproducible with good recovery values and can be applied in simultaneous determination of all mentioned compounds.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Chromatography A
T1  - Validation of liquid chromatographic method for analysis of lidocaine hydrochloride, dexamethasone acetate, calcium dobesilate, buthylhydroxyanisol and degradation product hydroquinone in suppositories and ointment
VL  - 1088
IS  - 1-2
SP  - 182
EP  - 186
DO  - 10.1016/j.chroma.2005.04.049
ER  - 

@article{
author = "Živanović, L and Zečević, Mira and Marković, S and Petrović, S and Ivanović, I",
year = "2005",
abstract = "In this paper, there was developed a sensitive, precise and accurate reversed-phase liquid chromatographic (RP-HPLC) method and validated for simultaneous determination of lidocaine hydrochloride, dexamethasone acetate (DA) and calcium dobesilate (CD) in suppositories and ointment. Also there was achieved a parallel analysis of buthylhydroxyanisol, as a preservative, and hydroquinone, as a degradation product of calcium dobesilate, present in these dosage forms. The relative standard deviation (RSD) values for all five compounds indicated a good precision and accuracy of the RP-HPLC method. Method is selective, sensitive and reproducible with good recovery values and can be applied in simultaneous determination of all mentioned compounds.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Chromatography A",
title = "Validation of liquid chromatographic method for analysis of lidocaine hydrochloride, dexamethasone acetate, calcium dobesilate, buthylhydroxyanisol and degradation product hydroquinone in suppositories and ointment",
volume = "1088",
number = "1-2",
pages = "182-186",
doi = "10.1016/j.chroma.2005.04.049"
}

Živanović, L., Zečević, M., Marković, S., Petrović, S.,& Ivanović, I.. (2005). Validation of liquid chromatographic method for analysis of lidocaine hydrochloride, dexamethasone acetate, calcium dobesilate, buthylhydroxyanisol and degradation product hydroquinone in suppositories and ointment. in Journal of Chromatography A
Elsevier Science BV, Amsterdam., 1088(1-2), 182-186.
https://doi.org/10.1016/j.chroma.2005.04.049

Živanović L, Zečević M, Marković S, Petrović S, Ivanović I. Validation of liquid chromatographic method for analysis of lidocaine hydrochloride, dexamethasone acetate, calcium dobesilate, buthylhydroxyanisol and degradation product hydroquinone in suppositories and ointment. in Journal of Chromatography A. 2005;1088(1-2):182-186.
doi:10.1016/j.chroma.2005.04.049 .

Živanović, L, Zečević, Mira, Marković, S, Petrović, S, Ivanović, I, "Validation of liquid chromatographic method for analysis of lidocaine hydrochloride, dexamethasone acetate, calcium dobesilate, buthylhydroxyanisol and degradation product hydroquinone in suppositories and ointment" in Journal of Chromatography A, 1088, no. 1-2 (2005):182-186,
https://doi.org/10.1016/j.chroma.2005.04.049 . .

TY  - JOUR
AU  - Živanović, L
AU  - Ivanović, I
AU  - Solomun, Ljiljana
AU  - Zečević, Mira
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/510
AB  - Cefuroxime is a broad-spectrum second-generation bactericidal cephalosporin antibiotic active against beta-lactamose-producing strains. Anti-cefuroxime, the geometric isomer of cefuroxime, might be present in cefuroxime dosage forms as a process-related impurity and possible degradation product. In the work discussed in this paper a precise and sensitive micellar liquid chromatographic (MLC) method for stability testing of cefuroxime axetil and anti-cefuroxime axetil in tablets, using benzoic acid as internal standard, was developed and validated. MLC was performed on an XTerra C-18 reversed-phase column at 50 degreesC with 8:92 (v/v) acetonitrile-20 mm sodium dodecyl sulphate, pH 2.5, as mobile phase at a flow rate of 1.5 mL min(-1). Detection was at 280 nm. Under these conditions the retention time and retention factor were of 6.65 min and 4.57, respectively, for cefuroxime axetil and 11.45 min and 8.59, respectively, for anti-cefuroxime axetil, indicating that the compounds were well separated. RSD values for quantification of cefuroxime axetil and anti-cefuroxime axetil were 0.39 and 1.7%, respectively, indicating the precision of the MLC method was good. The method is sensitive-LOD = 0.5 mug mL(-1) and LOQ = 1.5 mug mL(-1) for anti-cefuroxime axetil-and reproducible, with good recovery values.
PB  - Springer Heidelberg, Heidelberg
T2  - Chromatographia
T1  - Stability testing of cefuroxime in tablets by micellar liquid chromatography
VL  - 60
IS  - SUPPL.
DO  - 10.1365/s10337-004-0237-5
ER  - 

@article{
author = "Živanović, L and Ivanović, I and Solomun, Ljiljana and Zečević, Mira",
year = "2004",
abstract = "Cefuroxime is a broad-spectrum second-generation bactericidal cephalosporin antibiotic active against beta-lactamose-producing strains. Anti-cefuroxime, the geometric isomer of cefuroxime, might be present in cefuroxime dosage forms as a process-related impurity and possible degradation product. In the work discussed in this paper a precise and sensitive micellar liquid chromatographic (MLC) method for stability testing of cefuroxime axetil and anti-cefuroxime axetil in tablets, using benzoic acid as internal standard, was developed and validated. MLC was performed on an XTerra C-18 reversed-phase column at 50 degreesC with 8:92 (v/v) acetonitrile-20 mm sodium dodecyl sulphate, pH 2.5, as mobile phase at a flow rate of 1.5 mL min(-1). Detection was at 280 nm. Under these conditions the retention time and retention factor were of 6.65 min and 4.57, respectively, for cefuroxime axetil and 11.45 min and 8.59, respectively, for anti-cefuroxime axetil, indicating that the compounds were well separated. RSD values for quantification of cefuroxime axetil and anti-cefuroxime axetil were 0.39 and 1.7%, respectively, indicating the precision of the MLC method was good. The method is sensitive-LOD = 0.5 mug mL(-1) and LOQ = 1.5 mug mL(-1) for anti-cefuroxime axetil-and reproducible, with good recovery values.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Chromatographia",
title = "Stability testing of cefuroxime in tablets by micellar liquid chromatography",
volume = "60",
number = "SUPPL.",
doi = "10.1365/s10337-004-0237-5"
}

Živanović, L., Ivanović, I., Solomun, L.,& Zečević, M.. (2004). Stability testing of cefuroxime in tablets by micellar liquid chromatography. in Chromatographia
Springer Heidelberg, Heidelberg., 60(SUPPL.).
https://doi.org/10.1365/s10337-004-0237-5

Živanović L, Ivanović I, Solomun L, Zečević M. Stability testing of cefuroxime in tablets by micellar liquid chromatography. in Chromatographia. 2004;60(SUPPL.).
doi:10.1365/s10337-004-0237-5 .

Živanović, L, Ivanović, I, Solomun, Ljiljana, Zečević, Mira, "Stability testing of cefuroxime in tablets by micellar liquid chromatography" in Chromatographia, 60, no. SUPPL. (2004),
https://doi.org/10.1365/s10337-004-0237-5 . .

TY  - JOUR
AU  - Živanović, L
AU  - Ivanović, I
AU  - Vladimirov, S
AU  - Zečević, Mira
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/535
AB  - The optimal chromatographic conditions for the separation of the syn- and anti-geometric isomers of cefuroxime axetil applying RP-HPLC and micellar liquid chromatography (MLC) methods were investigated. The possibility to separate diastereoisomers of syn- and anti-cefuroxime axetil was observed. Investigations were performed using three columns, two classical silicas and one with hybrid particle technology. Three aqueous-organic and one micellar mobile phases were used. The best results were achieved using micellar mobile phase. Optimization study was performed using different micellar mobile phases. MLC method is sensitive and applicable in purity and stability testing.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
T1  - Investigation of chromatographic conditions for the separation of cefuroxime axetil and its geometric isomer
VL  - 800
IS  - 1-2
SP  - 175
EP  - 179
DO  - 10.1016/j.jchromb.2003.08.046
ER  - 

@article{
author = "Živanović, L and Ivanović, I and Vladimirov, S and Zečević, Mira",
year = "2004",
abstract = "The optimal chromatographic conditions for the separation of the syn- and anti-geometric isomers of cefuroxime axetil applying RP-HPLC and micellar liquid chromatography (MLC) methods were investigated. The possibility to separate diastereoisomers of syn- and anti-cefuroxime axetil was observed. Investigations were performed using three columns, two classical silicas and one with hybrid particle technology. Three aqueous-organic and one micellar mobile phases were used. The best results were achieved using micellar mobile phase. Optimization study was performed using different micellar mobile phases. MLC method is sensitive and applicable in purity and stability testing.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences",
title = "Investigation of chromatographic conditions for the separation of cefuroxime axetil and its geometric isomer",
volume = "800",
number = "1-2",
pages = "175-179",
doi = "10.1016/j.jchromb.2003.08.046"
}

Živanović, L., Ivanović, I., Vladimirov, S.,& Zečević, M.. (2004). Investigation of chromatographic conditions for the separation of cefuroxime axetil and its geometric isomer. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Elsevier Science BV, Amsterdam., 800(1-2), 175-179.
https://doi.org/10.1016/j.jchromb.2003.08.046

Živanović L, Ivanović I, Vladimirov S, Zečević M. Investigation of chromatographic conditions for the separation of cefuroxime axetil and its geometric isomer. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. 2004;800(1-2):175-179.
doi:10.1016/j.jchromb.2003.08.046 .

Živanović, L, Ivanović, I, Vladimirov, S, Zečević, Mira, "Investigation of chromatographic conditions for the separation of cefuroxime axetil and its geometric isomer" in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 800, no. 1-2 (2004):175-179,
https://doi.org/10.1016/j.jchromb.2003.08.046 . .

TY  - JOUR
AU  - Zečević, Mira
AU  - Živanović, L
AU  - Stojković, A
PY  - 2002
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/327
AB  - A new high-performance liquid chromatography (HPLC) method was developed for the quality control of pancuronium bromide and its degradation products. The HPLC method used a 5-mum Supelcogel ODP-50 (150X4 cm) column with acetonitrile-CH3OH-water-F3CCOOH (20.5:74.9:0.1, v/v) as the mobile phase (pH value 2.0 adjusted with trifluoroacetic acid) at a flow-rate 0.8 ml/min and UV detection at 210 nm. The Beer's law plots were found to be linear over the concentration range 0.4-1.2 mg/ml of pancuronium bromide and 0.04-0.08 mg/ml of desacetyl degradation products (R-2=0.9995). The RSD of the peak areas was 1.09% and the recovery was 102.43%. The RSD value shows good precision, acceptable accuracy and reproducibility of the new method for the determination of pancuronium bromide in presence of its desacetyl degradation products. The method is rapid and sensitive enough to be used for Pavulon injection analysis. 2002 Elsevier Science B.V. All rights reserved.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Chromatography A
T1  - Validation of a high-performance liquid chromatography method for the determination of pancuronium in Pavulon injections
VL  - 949
IS  - 1-2
SP  - 61
EP  - 64
DO  - 10.1016/S0021-9673(01)01552-7
ER  - 

@article{
author = "Zečević, Mira and Živanović, L and Stojković, A",
year = "2002",
abstract = "A new high-performance liquid chromatography (HPLC) method was developed for the quality control of pancuronium bromide and its degradation products. The HPLC method used a 5-mum Supelcogel ODP-50 (150X4 cm) column with acetonitrile-CH3OH-water-F3CCOOH (20.5:74.9:0.1, v/v) as the mobile phase (pH value 2.0 adjusted with trifluoroacetic acid) at a flow-rate 0.8 ml/min and UV detection at 210 nm. The Beer's law plots were found to be linear over the concentration range 0.4-1.2 mg/ml of pancuronium bromide and 0.04-0.08 mg/ml of desacetyl degradation products (R-2=0.9995). The RSD of the peak areas was 1.09% and the recovery was 102.43%. The RSD value shows good precision, acceptable accuracy and reproducibility of the new method for the determination of pancuronium bromide in presence of its desacetyl degradation products. The method is rapid and sensitive enough to be used for Pavulon injection analysis. 2002 Elsevier Science B.V. All rights reserved.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Chromatography A",
title = "Validation of a high-performance liquid chromatography method for the determination of pancuronium in Pavulon injections",
volume = "949",
number = "1-2",
pages = "61-64",
doi = "10.1016/S0021-9673(01)01552-7"
}

Zečević, M., Živanović, L.,& Stojković, A.. (2002). Validation of a high-performance liquid chromatography method for the determination of pancuronium in Pavulon injections. in Journal of Chromatography A
Elsevier Science BV, Amsterdam., 949(1-2), 61-64.
https://doi.org/10.1016/S0021-9673(01)01552-7

Zečević M, Živanović L, Stojković A. Validation of a high-performance liquid chromatography method for the determination of pancuronium in Pavulon injections. in Journal of Chromatography A. 2002;949(1-2):61-64.
doi:10.1016/S0021-9673(01)01552-7 .

Zečević, Mira, Živanović, L, Stojković, A, "Validation of a high-performance liquid chromatography method for the determination of pancuronium in Pavulon injections" in Journal of Chromatography A, 949, no. 1-2 (2002):61-64,
https://doi.org/10.1016/S0021-9673(01)01552-7 . .

TY  - JOUR
AU  - Zečević, Mira
AU  - Živanović, L
AU  - Agatonović-Kuštrin, Snežana
AU  - Minić, D
PY  - 2001
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/293
AB  - A multifactor optimisation technique is successfully applied to develop a new HPLC method in which methyldopa, hydrochlorothiazide and amiloride were analysed and determined on a C18 column with detection at 286 nm. The optimal conditions of HPLC separation were determined with the aid of the response surface diagram - 'window diagram'. The effect of simultaneously varying the pH, proportion aqueous acetic acidum and methanol in the mobile phase were studied to optimise the separation. The mobile phase composition that provides an acceptable resolution methyldopa, hydrochlorothiazide and amiloride in a short elution time is water-methanol (75:25) and pH 3.60. The k' values for methyldopa, hydrochlorothiazide and amiloride after optimisation were 1.40, 2.50 and 5.33, respectively. Relative retention (a) for ratio hydrochlorothiazide/methyldopa and amiloride/hydrochlorothiazide were 1.767 and 2.159, respectively. Correlation coefficients of the calibration curves for all analytes were greater than 0.995 and the R.S.D. values for the slope and the intercept with respect to the linearity were less than 2%. A method is applied for the quantitative analysis of Aatan(R) tablets (Lek-Ljubljana). The powdered tablets are extracted with methanol, containing caffeine as the internal standard and assayed by comparison of peak areas after liquid chromatography. The high recovery (for all analytes about 100%) and the low R.S.D. ( lt 2%) confirm good precision and reproducibility of the chromatographic method.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets
VL  - 24
IS  - 5-6
SP  - 1019
EP  - 1025
DO  - 10.1016/S0731-7085(00)00536-7
ER  - 

@article{
author = "Zečević, Mira and Živanović, L and Agatonović-Kuštrin, Snežana and Minić, D",
year = "2001",
abstract = "A multifactor optimisation technique is successfully applied to develop a new HPLC method in which methyldopa, hydrochlorothiazide and amiloride were analysed and determined on a C18 column with detection at 286 nm. The optimal conditions of HPLC separation were determined with the aid of the response surface diagram - 'window diagram'. The effect of simultaneously varying the pH, proportion aqueous acetic acidum and methanol in the mobile phase were studied to optimise the separation. The mobile phase composition that provides an acceptable resolution methyldopa, hydrochlorothiazide and amiloride in a short elution time is water-methanol (75:25) and pH 3.60. The k' values for methyldopa, hydrochlorothiazide and amiloride after optimisation were 1.40, 2.50 and 5.33, respectively. Relative retention (a) for ratio hydrochlorothiazide/methyldopa and amiloride/hydrochlorothiazide were 1.767 and 2.159, respectively. Correlation coefficients of the calibration curves for all analytes were greater than 0.995 and the R.S.D. values for the slope and the intercept with respect to the linearity were less than 2%. A method is applied for the quantitative analysis of Aatan(R) tablets (Lek-Ljubljana). The powdered tablets are extracted with methanol, containing caffeine as the internal standard and assayed by comparison of peak areas after liquid chromatography. The high recovery (for all analytes about 100%) and the low R.S.D. ( lt 2%) confirm good precision and reproducibility of the chromatographic method.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets",
volume = "24",
number = "5-6",
pages = "1019-1025",
doi = "10.1016/S0731-7085(00)00536-7"
}

Zečević, M., Živanović, L., Agatonović-Kuštrin, S.,& Minić, D.. (2001). The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets. in Journal of Pharmaceutical and Biomedical Analysis
Pergamon-Elsevier Science Ltd, Oxford., 24(5-6), 1019-1025.
https://doi.org/10.1016/S0731-7085(00)00536-7

Zečević M, Živanović L, Agatonović-Kuštrin S, Minić D. The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets. in Journal of Pharmaceutical and Biomedical Analysis. 2001;24(5-6):1019-1025.
doi:10.1016/S0731-7085(00)00536-7 .

Zečević, Mira, Živanović, L, Agatonović-Kuštrin, Snežana, Minić, D, "The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets" in Journal of Pharmaceutical and Biomedical Analysis, 24, no. 5-6 (2001):1019-1025,
https://doi.org/10.1016/S0731-7085(00)00536-7 . .

TY  - JOUR
AU  - Agatonović-Kuštrin, Snežana
AU  - Zečević, Mira
AU  - Živanović, L
PY  - 1999
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/228
AB  - Structure-retention relationship study, conducted by RP-HPLC, was used to investigate physical chemical parameters related to the RP retention times of amiloride, hydrochlorothiazide and methyldopa in order to predict the separation of amiloride and methylclothiazide from Lometazid(R) tablets. Retention data were obtained with an ODS column using a mobile phase methanol-water (pH adjusted with phosphoric acid). Physical chemical properties were calculated directly from the molecular structure. Artificial neural networks (ANNs) were used to correlate chromatograms retention times with mobile phase composition and pH, and with physical chemical properties of amiloride, hydrochlorothiazide and methyldopa and to predict separation of amiloride and methylclothiazide from Lometazid(R) tablets. Sensitivity analysis was performed to interpret the meaning of the descriptors included in the models. Results confirmed the dominant role of the polar modifier in such chromatographic systems. Within a series of solutes chromatographed under identical conditions, the retention parameters could be approximated by a non-linear combination of log P, log D, pK(a), surface tension, parachor, molar volume and to minor extend by polarisability, rexractivity index and density. This study has demonstrated that the use ANNs techniques can result in much more efficient use of experimental information. As HPLC is the most popular analytical technique, improvements in HPLC methods development can yield significant gains in the overall analytical effort. The ANNs extension presented could be the method of choice in some advanced research settings and serves as an indication of the broad potential of neural networks in chromatography analysis.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Use of ANN modelling in structure-retention relationships of diuretics in RP-HPLC
VL  - 21
IS  - 1
SP  - 95
EP  - 103
DO  - 10.1016/S0731-7085(99)00133-8
ER  - 

@article{
author = "Agatonović-Kuštrin, Snežana and Zečević, Mira and Živanović, L",
year = "1999",
abstract = "Structure-retention relationship study, conducted by RP-HPLC, was used to investigate physical chemical parameters related to the RP retention times of amiloride, hydrochlorothiazide and methyldopa in order to predict the separation of amiloride and methylclothiazide from Lometazid(R) tablets. Retention data were obtained with an ODS column using a mobile phase methanol-water (pH adjusted with phosphoric acid). Physical chemical properties were calculated directly from the molecular structure. Artificial neural networks (ANNs) were used to correlate chromatograms retention times with mobile phase composition and pH, and with physical chemical properties of amiloride, hydrochlorothiazide and methyldopa and to predict separation of amiloride and methylclothiazide from Lometazid(R) tablets. Sensitivity analysis was performed to interpret the meaning of the descriptors included in the models. Results confirmed the dominant role of the polar modifier in such chromatographic systems. Within a series of solutes chromatographed under identical conditions, the retention parameters could be approximated by a non-linear combination of log P, log D, pK(a), surface tension, parachor, molar volume and to minor extend by polarisability, rexractivity index and density. This study has demonstrated that the use ANNs techniques can result in much more efficient use of experimental information. As HPLC is the most popular analytical technique, improvements in HPLC methods development can yield significant gains in the overall analytical effort. The ANNs extension presented could be the method of choice in some advanced research settings and serves as an indication of the broad potential of neural networks in chromatography analysis.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Use of ANN modelling in structure-retention relationships of diuretics in RP-HPLC",
volume = "21",
number = "1",
pages = "95-103",
doi = "10.1016/S0731-7085(99)00133-8"
}

Agatonović-Kuštrin, S., Zečević, M.,& Živanović, L.. (1999). Use of ANN modelling in structure-retention relationships of diuretics in RP-HPLC. in Journal of Pharmaceutical and Biomedical Analysis
Pergamon-Elsevier Science Ltd, Oxford., 21(1), 95-103.
https://doi.org/10.1016/S0731-7085(99)00133-8

Agatonović-Kuštrin S, Zečević M, Živanović L. Use of ANN modelling in structure-retention relationships of diuretics in RP-HPLC. in Journal of Pharmaceutical and Biomedical Analysis. 1999;21(1):95-103.
doi:10.1016/S0731-7085(99)00133-8 .

Agatonović-Kuštrin, Snežana, Zečević, Mira, Živanović, L, "Use of ANN modelling in structure-retention relationships of diuretics in RP-HPLC" in Journal of Pharmaceutical and Biomedical Analysis, 21, no. 1 (1999):95-103,
https://doi.org/10.1016/S0731-7085(99)00133-8 . .

TY  - JOUR
AU  - Agatonović-Kuštrin, Snežana
AU  - Zečević, Mira
AU  - Živanović, L
AU  - Tucker, I.G
PY  - 1998
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/187
AB  - The use of artificial neural networks (ANNs) for response surface modelling in HPLC method development for amiloride and methychlothiazide separation is reported. The independent input variables were pH and methanol percentage in mobile phase. The outputs were capacity factors. The results were compared with a statistical method (multiple nonlinear regression analysis). Networks were able to predict the experimental responses more accurately than the regression analysis.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Application of artificial neural networks in HPLC method development
VL  - 17
IS  - 1
SP  - 69
EP  - 76
DO  - 10.1016/S0731-7085(97)00170-2
ER  - 

@article{
author = "Agatonović-Kuštrin, Snežana and Zečević, Mira and Živanović, L and Tucker, I.G",
year = "1998",
abstract = "The use of artificial neural networks (ANNs) for response surface modelling in HPLC method development for amiloride and methychlothiazide separation is reported. The independent input variables were pH and methanol percentage in mobile phase. The outputs were capacity factors. The results were compared with a statistical method (multiple nonlinear regression analysis). Networks were able to predict the experimental responses more accurately than the regression analysis.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Application of artificial neural networks in HPLC method development",
volume = "17",
number = "1",
pages = "69-76",
doi = "10.1016/S0731-7085(97)00170-2"
}

Agatonović-Kuštrin, S., Zečević, M., Živanović, L.,& Tucker, I.G. (1998). Application of artificial neural networks in HPLC method development. in Journal of Pharmaceutical and Biomedical Analysis
Pergamon-Elsevier Science Ltd, Oxford., 17(1), 69-76.
https://doi.org/10.1016/S0731-7085(97)00170-2

Agatonović-Kuštrin S, Zečević M, Živanović L, Tucker I. Application of artificial neural networks in HPLC method development. in Journal of Pharmaceutical and Biomedical Analysis. 1998;17(1):69-76.
doi:10.1016/S0731-7085(97)00170-2 .

Agatonović-Kuštrin, Snežana, Zečević, Mira, Živanović, L, Tucker, I.G, "Application of artificial neural networks in HPLC method development" in Journal of Pharmaceutical and Biomedical Analysis, 17, no. 1 (1998):69-76,
https://doi.org/10.1016/S0731-7085(97)00170-2 . .

TY  - JOUR
AU  - Agatonović-Kuštrin, Snežana
AU  - Zečević, Mira
AU  - Živanović, L
AU  - Tucker, I.G
PY  - 1998
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/183
AB  - The usefulness of artificial neural networks for response surface modeling in HPLC optimization is compared with multiple regression methods. The results show that neural networks offer promising possibilities in HPLC method development. The predicted capacity factors of analytes were better to those obtained with multiple regression method.
PB  - Elsevier Science BV, Amsterdam
T2  - Analytica Chimica Acta
T1  - Application of neural networks for response surface modeling in HPLC optimization
VL  - 364
IS  - 1-3
SP  - 265
EP  - 273
DO  - 10.1016/S0003-2670(98)00121-4
ER  - 

@article{
author = "Agatonović-Kuštrin, Snežana and Zečević, Mira and Živanović, L and Tucker, I.G",
year = "1998",
abstract = "The usefulness of artificial neural networks for response surface modeling in HPLC optimization is compared with multiple regression methods. The results show that neural networks offer promising possibilities in HPLC method development. The predicted capacity factors of analytes were better to those obtained with multiple regression method.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Analytica Chimica Acta",
title = "Application of neural networks for response surface modeling in HPLC optimization",
volume = "364",
number = "1-3",
pages = "265-273",
doi = "10.1016/S0003-2670(98)00121-4"
}

Agatonović-Kuštrin, S., Zečević, M., Živanović, L.,& Tucker, I.G. (1998). Application of neural networks for response surface modeling in HPLC optimization. in Analytica Chimica Acta
Elsevier Science BV, Amsterdam., 364(1-3), 265-273.
https://doi.org/10.1016/S0003-2670(98)00121-4

Agatonović-Kuštrin S, Zečević M, Živanović L, Tucker I. Application of neural networks for response surface modeling in HPLC optimization. in Analytica Chimica Acta. 1998;364(1-3):265-273.
doi:10.1016/S0003-2670(98)00121-4 .

Agatonović-Kuštrin, Snežana, Zečević, Mira, Živanović, L, Tucker, I.G, "Application of neural networks for response surface modeling in HPLC optimization" in Analytica Chimica Acta, 364, no. 1-3 (1998):265-273,
https://doi.org/10.1016/S0003-2670(98)00121-4 . .

TY  - JOUR
AU  - Agatonović-Kuštrin, Snežana
AU  - Živanović, L
AU  - Zečević, Mira
AU  - Radulović, D
PY  - 1997
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/154
AB  - A multifactor optimisation technique is successfully applied to develop a new spectrophotometric method in which diclofenac sodium is analysed and determined as it's Fe(III) complex. The effect of simultaneously varying the pH, ionic strength and concentration of colour reagents in the reaction mixture were studied. A four-variable two-level factorial design was used to investigate the significance of each variable and interactions between them. A response surface design was used to optimise complex formation and extraction. It was established that diclofenac reacts with Fe(III) chloride, in the presence of ammonium thiocyanate, in the pH range 4.2-6.5, forming a red chloroform extractable (2:1) complex with maximum absorbance at 481 nm. By applying the methods of Sommer and Job involving non-equimolar solutions the conditional stability constant of the complex, at the optimum pH of 6.0 and an ionic strength mu = 0.19M, was found to be 10(6.4). Good agreement with Beer's law was found for diclofenac concentrations up to mmol l(-1). The nominal percent recovery of diclofenac was 98.8% (n = 10). The lower limit of sensitivity of the method was found to be 14.7 mu g ml(-1).
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Spectrophotometric study of diclofenac-Fe(III) complex
VL  - 16
IS  - 1
SP  - 147
EP  - 153
DO  - 10.1016/S0731-7085(97)00016-2
ER  - 

@article{
author = "Agatonović-Kuštrin, Snežana and Živanović, L and Zečević, Mira and Radulović, D",
year = "1997",
abstract = "A multifactor optimisation technique is successfully applied to develop a new spectrophotometric method in which diclofenac sodium is analysed and determined as it's Fe(III) complex. The effect of simultaneously varying the pH, ionic strength and concentration of colour reagents in the reaction mixture were studied. A four-variable two-level factorial design was used to investigate the significance of each variable and interactions between them. A response surface design was used to optimise complex formation and extraction. It was established that diclofenac reacts with Fe(III) chloride, in the presence of ammonium thiocyanate, in the pH range 4.2-6.5, forming a red chloroform extractable (2:1) complex with maximum absorbance at 481 nm. By applying the methods of Sommer and Job involving non-equimolar solutions the conditional stability constant of the complex, at the optimum pH of 6.0 and an ionic strength mu = 0.19M, was found to be 10(6.4). Good agreement with Beer's law was found for diclofenac concentrations up to mmol l(-1). The nominal percent recovery of diclofenac was 98.8% (n = 10). The lower limit of sensitivity of the method was found to be 14.7 mu g ml(-1).",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Spectrophotometric study of diclofenac-Fe(III) complex",
volume = "16",
number = "1",
pages = "147-153",
doi = "10.1016/S0731-7085(97)00016-2"
}

Agatonović-Kuštrin, S., Živanović, L., Zečević, M.,& Radulović, D.. (1997). Spectrophotometric study of diclofenac-Fe(III) complex. in Journal of Pharmaceutical and Biomedical Analysis
Pergamon-Elsevier Science Ltd, Oxford., 16(1), 147-153.
https://doi.org/10.1016/S0731-7085(97)00016-2

Agatonović-Kuštrin S, Živanović L, Zečević M, Radulović D. Spectrophotometric study of diclofenac-Fe(III) complex. in Journal of Pharmaceutical and Biomedical Analysis. 1997;16(1):147-153.
doi:10.1016/S0731-7085(97)00016-2 .

Agatonović-Kuštrin, Snežana, Živanović, L, Zečević, Mira, Radulović, D, "Spectrophotometric study of diclofenac-Fe(III) complex" in Journal of Pharmaceutical and Biomedical Analysis, 16, no. 1 (1997):147-153,
https://doi.org/10.1016/S0731-7085(97)00016-2 . .