TY  - CONF
AU  - Gledović, Ana
AU  - Ilić, Tanja
AU  - Savić, Snežana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5189
AB  - Nanoemulsions (NEs), especially those prepared by low-
energy processes, have recently been proposed as
promising carriers for natural products such as plant oils and
extracts. ...
PB  - APGI – “Association de Pharmacie Galénique Industrielle”
C3  - 6th Symposium Skin and Formulation, 2-3 October, 2023, Nantes - France
T1  - Eye irritation potential of red raspberry seed oil after nanoemulsification: assessment with the HET-CAM test
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5189
ER  - 

@conference{
author = "Gledović, Ana and Ilić, Tanja and Savić, Snežana",
year = "2023",
abstract = "Nanoemulsions (NEs), especially those prepared by low-
energy processes, have recently been proposed as
promising carriers for natural products such as plant oils and
extracts. ...",
publisher = "APGI – “Association de Pharmacie Galénique Industrielle”",
journal = "6th Symposium Skin and Formulation, 2-3 October, 2023, Nantes - France",
title = "Eye irritation potential of red raspberry seed oil after nanoemulsification: assessment with the HET-CAM test",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5189"
}

Gledović, A., Ilić, T.,& Savić, S.. (2023). Eye irritation potential of red raspberry seed oil after nanoemulsification: assessment with the HET-CAM test. in 6th Symposium Skin and Formulation, 2-3 October, 2023, Nantes - France
APGI – “Association de Pharmacie Galénique Industrielle”..
https://hdl.handle.net/21.15107/rcub_farfar_5189

Gledović A, Ilić T, Savić S. Eye irritation potential of red raspberry seed oil after nanoemulsification: assessment with the HET-CAM test. in 6th Symposium Skin and Formulation, 2-3 October, 2023, Nantes - France. 2023;.
https://hdl.handle.net/21.15107/rcub_farfar_5189 .

Gledović, Ana, Ilić, Tanja, Savić, Snežana, "Eye irritation potential of red raspberry seed oil after nanoemulsification: assessment with the HET-CAM test" in 6th Symposium Skin and Formulation, 2-3 October, 2023, Nantes - France (2023),
https://hdl.handle.net/21.15107/rcub_farfar_5189 .

TY  - CONF
AU  - Ilić, Tanja
AU  - Gledović, Ana
AU  - Dobričić, Vladimir
AU  - Pantelić, Ivana
AU  - Savić, Snežana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5182
AB  - Repeated sun-exposure is one of the main sources of oxidative stress in the skin, which is responsible for the majority of age-associated skin conditions. ...
PB  - APGI – “Association de Pharmacie Galénique Industrielle”
C3  - 6th Symposium Skin and Formulation, 2-3 October, 2023, Nantes - France
T1  - Optimization of ORAC assay combined with in vivo tape stripping for the assessment of antioxidant efficacy of cosmetic formulations
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5182
ER  - 

@conference{
author = "Ilić, Tanja and Gledović, Ana and Dobričić, Vladimir and Pantelić, Ivana and Savić, Snežana",
year = "2023",
abstract = "Repeated sun-exposure is one of the main sources of oxidative stress in the skin, which is responsible for the majority of age-associated skin conditions. ...",
publisher = "APGI – “Association de Pharmacie Galénique Industrielle”",
journal = "6th Symposium Skin and Formulation, 2-3 October, 2023, Nantes - France",
title = "Optimization of ORAC assay combined with in vivo tape stripping for the assessment of antioxidant efficacy of cosmetic formulations",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5182"
}

Ilić, T., Gledović, A., Dobričić, V., Pantelić, I.,& Savić, S.. (2023). Optimization of ORAC assay combined with in vivo tape stripping for the assessment of antioxidant efficacy of cosmetic formulations. in 6th Symposium Skin and Formulation, 2-3 October, 2023, Nantes - France
APGI – “Association de Pharmacie Galénique Industrielle”..
https://hdl.handle.net/21.15107/rcub_farfar_5182

Ilić T, Gledović A, Dobričić V, Pantelić I, Savić S. Optimization of ORAC assay combined with in vivo tape stripping for the assessment of antioxidant efficacy of cosmetic formulations. in 6th Symposium Skin and Formulation, 2-3 October, 2023, Nantes - France. 2023;.
https://hdl.handle.net/21.15107/rcub_farfar_5182 .

Ilić, Tanja, Gledović, Ana, Dobričić, Vladimir, Pantelić, Ivana, Savić, Snežana, "Optimization of ORAC assay combined with in vivo tape stripping for the assessment of antioxidant efficacy of cosmetic formulations" in 6th Symposium Skin and Formulation, 2-3 October, 2023, Nantes - France (2023),
https://hdl.handle.net/21.15107/rcub_farfar_5182 .

TY  - CONF
AU  - Gledović, Ana
AU  - Janošević-Ležaić, Aleksandra
AU  - Savić, Snežana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4574
AB  - In the past twenty years plant oils derived from food industry byproducts have been
in focus due to the ecological and economic reasons. For example, raspberry seed oil – RSO
(Rubus idaeus, Rosaceae), abundant with bioactive compounds such as polyunsaturated fatty
acids, carotenoids, tocopherols and phenolic compounds, is recommended for antioxidant and
UV-protective skincare (1). RSO application is limited by poor oxidative stability, thus this
research aimed at its stabilization with nanoemulsion – NE carrier. Firstly, total carotenoid
and phenolic content of RSO was determined, followed by determination of its oxidative
status by tracking relevant parameters (peroxide value – PV, p-anisidine value – PA, and
thiobarbituric reactive substances – TBARS) (2) during one month of storage. NEs were
prepared with the polyglycerol ester-based natural surfactants by employing phase
inversion composition method, with or without RSO and antioxidants: synthetic (butylated
hydroxytoluene – BHT or ethylenediaminetetraacetic acid, disodium salt – Na 2EDTA), or
sessile oak acorn extract (Quercus petraea, Fagaceae). NEs' physiochemical characterization
included droplet size distribution analysis, electrical conductivity and pH value
measurements, followed by measurement of primary and secondary oxidation products
(lipid hydroperoxides – LH and TBARS, respectively). It was found that RSOs' oxidative
stability was significantly improved when stored at refrigerator after opening or by adding
BHT (PV˂10, PA˂6, % INH TBARS >80%). NEs were semitransparent (with very small
droplets ˂70 nm) and stable at 25 and 40ºC, while drastic formation of oxidative products
was avoided by employing BHT or Na2EDTA, or by using oak extract as natural stabilizer.
AB  - U poslednjih dvadeset godina, usled ekoloških i ekonomskih razloga u fokusu
istraživanja su biljna ulja koja se dobijaju od nusproizvoda prehrambene industrjje. Na
primer, ulje semena maline (Rubus idaeus, Rosaceae), koje obiluje bioaktivnim sastojcima
kao što su polinezasićene masne kiseline (linolna i linolenska), karotenoidi, tokoferoli i
fenolna jedinjenja, preporučuje se za negu,antioksidativnu i UV-zaštitu kože (1). Primenu ulja
maline ograničava loša oksidativna stabilnost, te je cilj ovog istraživanja bila njegova
stabilizacija primenom nanoemulzije (NE), i/ili antioksidanata. Prvo je određivan sadržaj
ukupnih karotenoida i fenola, a zatim oksidativni status ulja maline praćenjem relevantnih
parametara (peroksidni broj – PB, p-anisidinski broj – PA i sadržaj reaktivnih supstanci
tiobarbiturne kiseline – TBARS) (2), tokom mesec dana čuvanja. NEs su pripremljene na bazi
novijih prirodnih poliglicerilskih estara pomoću metode inverzije faza, bezili sa dodatkom ulja
semena maline i antioksidanata: sintetskih (butilhidroksitoluen – BHT ili dinatrijumova so
etilendiamintetrasirćetne kiseline – Na 2EDTA), ili prirodnog ekstrakta žira hrasta kitnjaka
(Quercus petraea, Fagaceae). Fizičkohemijska karakterizacija NEs izvršena je analizom
distribucije veličina kapi, merenjem električne provodljivosti i pH vrednosti, te određivanjem
primarnih i sekundarnih produkata oksidacije (lipidnih hidroperoksida – LH i TBARS
supstanci, redom). Nađeno je da se oksidativna stabilnost ulja maline značajno poboljšala
čuvanjem u frižideru nakon otvaranja ili dodatkom BHT-a (PB˂10, PA˂6, % INH TBARS >80%).
Dobijene NEs su bile polutransparentne (usled malih veličina kapi ˂70 nm) i stabilne na 25 i
40ºC, a drastičan porast oksidacionih produkata je uspešno inhibiran upotrebom BHT-a ili
Na 2 EDTA, kao i pomoćuekstrakta hrasta kitnjaka kao potencijalnog prirodnog stabilizatora.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Influence of nanoemulsfication, antioxidants and temperature on oxidative stability of red rapsberry seed oil for skin application
T1  - Procena uticaja nanoemulzifikacije, antioksidanata i temperature na oksidativnu stabilnost ulja semena maline za primenu na koži
VL  - 72
IS  - 4 suplement
SP  - S420
EP  - S421
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4574
ER  - 

@conference{
author = "Gledović, Ana and Janošević-Ležaić, Aleksandra and Savić, Snežana",
year = "2022",
abstract = "In the past twenty years plant oils derived from food industry byproducts have been
in focus due to the ecological and economic reasons. For example, raspberry seed oil – RSO
(Rubus idaeus, Rosaceae), abundant with bioactive compounds such as polyunsaturated fatty
acids, carotenoids, tocopherols and phenolic compounds, is recommended for antioxidant and
UV-protective skincare (1). RSO application is limited by poor oxidative stability, thus this
research aimed at its stabilization with nanoemulsion – NE carrier. Firstly, total carotenoid
and phenolic content of RSO was determined, followed by determination of its oxidative
status by tracking relevant parameters (peroxide value – PV, p-anisidine value – PA, and
thiobarbituric reactive substances – TBARS) (2) during one month of storage. NEs were
prepared with the polyglycerol ester-based natural surfactants by employing phase
inversion composition method, with or without RSO and antioxidants: synthetic (butylated
hydroxytoluene – BHT or ethylenediaminetetraacetic acid, disodium salt – Na 2EDTA), or
sessile oak acorn extract (Quercus petraea, Fagaceae). NEs' physiochemical characterization
included droplet size distribution analysis, electrical conductivity and pH value
measurements, followed by measurement of primary and secondary oxidation products
(lipid hydroperoxides – LH and TBARS, respectively). It was found that RSOs' oxidative
stability was significantly improved when stored at refrigerator after opening or by adding
BHT (PV˂10, PA˂6, % INH TBARS >80%). NEs were semitransparent (with very small
droplets ˂70 nm) and stable at 25 and 40ºC, while drastic formation of oxidative products
was avoided by employing BHT or Na2EDTA, or by using oak extract as natural stabilizer., U poslednjih dvadeset godina, usled ekoloških i ekonomskih razloga u fokusu
istraživanja su biljna ulja koja se dobijaju od nusproizvoda prehrambene industrjje. Na
primer, ulje semena maline (Rubus idaeus, Rosaceae), koje obiluje bioaktivnim sastojcima
kao što su polinezasićene masne kiseline (linolna i linolenska), karotenoidi, tokoferoli i
fenolna jedinjenja, preporučuje se za negu,antioksidativnu i UV-zaštitu kože (1). Primenu ulja
maline ograničava loša oksidativna stabilnost, te je cilj ovog istraživanja bila njegova
stabilizacija primenom nanoemulzije (NE), i/ili antioksidanata. Prvo je određivan sadržaj
ukupnih karotenoida i fenola, a zatim oksidativni status ulja maline praćenjem relevantnih
parametara (peroksidni broj – PB, p-anisidinski broj – PA i sadržaj reaktivnih supstanci
tiobarbiturne kiseline – TBARS) (2), tokom mesec dana čuvanja. NEs su pripremljene na bazi
novijih prirodnih poliglicerilskih estara pomoću metode inverzije faza, bezili sa dodatkom ulja
semena maline i antioksidanata: sintetskih (butilhidroksitoluen – BHT ili dinatrijumova so
etilendiamintetrasirćetne kiseline – Na 2EDTA), ili prirodnog ekstrakta žira hrasta kitnjaka
(Quercus petraea, Fagaceae). Fizičkohemijska karakterizacija NEs izvršena je analizom
distribucije veličina kapi, merenjem električne provodljivosti i pH vrednosti, te određivanjem
primarnih i sekundarnih produkata oksidacije (lipidnih hidroperoksida – LH i TBARS
supstanci, redom). Nađeno je da se oksidativna stabilnost ulja maline značajno poboljšala
čuvanjem u frižideru nakon otvaranja ili dodatkom BHT-a (PB˂10, PA˂6, % INH TBARS >80%).
Dobijene NEs su bile polutransparentne (usled malih veličina kapi ˂70 nm) i stabilne na 25 i
40ºC, a drastičan porast oksidacionih produkata je uspešno inhibiran upotrebom BHT-a ili
Na 2 EDTA, kao i pomoćuekstrakta hrasta kitnjaka kao potencijalnog prirodnog stabilizatora.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Influence of nanoemulsfication, antioxidants and temperature on oxidative stability of red rapsberry seed oil for skin application, Procena uticaja nanoemulzifikacije, antioksidanata i temperature na oksidativnu stabilnost ulja semena maline za primenu na koži",
volume = "72",
number = "4 suplement",
pages = "S420-S421",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4574"
}

Gledović, A., Janošević-Ležaić, A.,& Savić, S.. (2022). Influence of nanoemulsfication, antioxidants and temperature on oxidative stability of red rapsberry seed oil for skin application. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S420-S421.
https://hdl.handle.net/21.15107/rcub_farfar_4574

Gledović A, Janošević-Ležaić A, Savić S. Influence of nanoemulsfication, antioxidants and temperature on oxidative stability of red rapsberry seed oil for skin application. in Arhiv za farmaciju. 2022;72(4 suplement):S420-S421.
https://hdl.handle.net/21.15107/rcub_farfar_4574 .

Gledović, Ana, Janošević-Ležaić, Aleksandra, Savić, Snežana, "Influence of nanoemulsfication, antioxidants and temperature on oxidative stability of red rapsberry seed oil for skin application" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S420-S421,
https://hdl.handle.net/21.15107/rcub_farfar_4574 .

TY  - JOUR
AU  - Gledović, Ana
AU  - Janošević-Ležaić, Aleksandra
AU  - Tamburić, Slobodanka
AU  - Savić, Snežana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4300
AB  - The aim of this study was to assess and improve the oxidative stability of red raspberry seed oil–RO, a potential topical ingredient derived from food industry by-products, on its own and when incorporated in low energy nanoemulsion (NE). The RO’s oxidative stability was assessed at 5, 25, and 40 °C during one month of storage and expressed in: peroxide value, p-anisidine, and thiobarbituric reactive substances—TBARS value, while for NEs, lipid hydroperoxides and TBARS values were monitored. Both synthetic (butylated hydroxytoluene—BHT and ethylenediaminetetraacetic acid—EDTA), and natural (oregano essential oil—ORE and oak fruit extract—OAK) antioxidants were used. Pure RO and RO with BHT or ORE were stable at 5 °C and 25 °C, but at 40 °C BHT showed only moderate protection, while ORE was prooxidant. NEs prepared with new biodegradable polyglycerol esters-based surfactants, with droplet sizes of < 50 nm and narrow size distribution, showed improved physicochemical stability at room temperature, and especially at 40 °C, compared to NEs with polysorbate 80, which required the addition of antioxidants to preserve their stability. Natural antioxidants ORE and OAK were compatible with all NEs; therefore, their use is proposed as an effective alternative to synthetic antioxidants.
PB  - MDPI
T2  - Antioxidants
T1  - Red Raspberry Seed Oil Low Energy Nanoemulsions: Influence of Surfactants, Antioxidants, and Temperature on Oxidative Stability
VL  - 11
IS  - 10
DO  - 10.3390/antiox11101898
ER  - 

@article{
author = "Gledović, Ana and Janošević-Ležaić, Aleksandra and Tamburić, Slobodanka and Savić, Snežana",
year = "2022",
abstract = "The aim of this study was to assess and improve the oxidative stability of red raspberry seed oil–RO, a potential topical ingredient derived from food industry by-products, on its own and when incorporated in low energy nanoemulsion (NE). The RO’s oxidative stability was assessed at 5, 25, and 40 °C during one month of storage and expressed in: peroxide value, p-anisidine, and thiobarbituric reactive substances—TBARS value, while for NEs, lipid hydroperoxides and TBARS values were monitored. Both synthetic (butylated hydroxytoluene—BHT and ethylenediaminetetraacetic acid—EDTA), and natural (oregano essential oil—ORE and oak fruit extract—OAK) antioxidants were used. Pure RO and RO with BHT or ORE were stable at 5 °C and 25 °C, but at 40 °C BHT showed only moderate protection, while ORE was prooxidant. NEs prepared with new biodegradable polyglycerol esters-based surfactants, with droplet sizes of < 50 nm and narrow size distribution, showed improved physicochemical stability at room temperature, and especially at 40 °C, compared to NEs with polysorbate 80, which required the addition of antioxidants to preserve their stability. Natural antioxidants ORE and OAK were compatible with all NEs; therefore, their use is proposed as an effective alternative to synthetic antioxidants.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Red Raspberry Seed Oil Low Energy Nanoemulsions: Influence of Surfactants, Antioxidants, and Temperature on Oxidative Stability",
volume = "11",
number = "10",
doi = "10.3390/antiox11101898"
}

Gledović, A., Janošević-Ležaić, A., Tamburić, S.,& Savić, S.. (2022). Red Raspberry Seed Oil Low Energy Nanoemulsions: Influence of Surfactants, Antioxidants, and Temperature on Oxidative Stability. in Antioxidants
MDPI., 11(10).
https://doi.org/10.3390/antiox11101898

Gledović A, Janošević-Ležaić A, Tamburić S, Savić S. Red Raspberry Seed Oil Low Energy Nanoemulsions: Influence of Surfactants, Antioxidants, and Temperature on Oxidative Stability. in Antioxidants. 2022;11(10).
doi:10.3390/antiox11101898 .

Gledović, Ana, Janošević-Ležaić, Aleksandra, Tamburić, Slobodanka, Savić, Snežana, "Red Raspberry Seed Oil Low Energy Nanoemulsions: Influence of Surfactants, Antioxidants, and Temperature on Oxidative Stability" in Antioxidants, 11, no. 10 (2022),
https://doi.org/10.3390/antiox11101898 . .

TY  - JOUR
AU  - Gledović, Ana
AU  - Bajuk-Bogdanović, Danica
AU  - Uskoković-Marković, Snežana
AU  - Pavun, Leposava
AU  - Savić, Snežana
AU  - Janošević-Ležaić, Aleksandra
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4170
AB  - In this study several essential oils (EOs): basil - BA, lemon balm - LB and oregano - OR were incorporated into nanoemulsions (NEs) as prospective carriers for natural and sensitive bioactives. NEs were prepared via the phase inversion composition (PIC) method, which is an energy-efficient cold process. Physicochemical stability of NEs was confirmed by particle size distribution analysis, electrical conductivity and pH value measurements, as well as by optical microscopy observations. The type of EO and the surfactant and oil mix concentration were found to be crucial factors governing the NE properties and stability. Raman spectra of the EOs confirmed main active ingredients and provided detection of interactions with the nanocarrier, which is a novel application of this technique. The antioxidant activity towards DPPH radical in methanol was concentration-dependent with a similar trend for individual oils and oil-loaded NEs (OR> LB> BA). However, the ABTS test in an aqueous medium revealed notable change in the order of activity after EO nanonisation at higher EO concentrations. Overall, it was found that OR-NE was the most effective and stable system, since OR acted as a co-stabiliser in the NE formulation, and its remarkably high antioxidant activity was successfully preserved during 6 months of storage.
AB  - U ovoj studiji nekoliko različitih etarskih ulja (EU): bosiljak – BO, matičnjak – MA i origano – OR inkor-
porirano je u nanoemulzije (NE) kao potencijalne nosače za prirodne i osetljive bioaktivne sastojke. NE
su pripremljene pomoću metode inverzije faza (engl. phase inversion composition – PIC method) koja
predstavlja niskoenergetski i ekonomičan postupak izrade. Fizičkohemijska stabilnost nanoemulzija
potvrđena je analizom raspodele veličina kapi, merenjem električne provodljivosti i pH vrednosti, kao i
optičkom mikroskopijom. Nađeno je da su vrsta EU i koncentracija smeše surfaktanata i uljane faze dva
ključna faktora koja su uticala na karakteristike i stabilnost dobijenih emulzija. Tehnikom Ramanske
spektroskopije potvrđeni su glavni aktivni sastojci etarskih ulja i detektovane su moguće interakcije sa
nanonosačem, što predstavlja noviju primenu pomenute tehnike. Pokazano je da antioksidantna
aktivnost prema DPPH radikalu u metanolu zavisi od koncentracije, sa sličnim trendom za čista etarska
ulja i za nanoemulzije sa uljima (OR> MA> BO). Međutim, ABTS test u vodenoj sredini pokazao je izrazite
promene u redosledu aktivnosti sa povećanjem koncentracije EU i nakon nanonizacije EU. Generalno,
dokazano je da je OR-NE najefikasniji i najstabiliniji sistem, s obzirom da je ulje origana ispoljilo ulogu
kostabilizatora u formulaciji, uz istovremeno očuvanje njegove veoma visoke antioksidantne aktivnosti
u obliku nanoemulzije, tokom 6 meseci čuvanja.
PB  - Association of the Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Low energy nanoemulsions as carriers for essential oils in topical formulations for antioxidant skin protection
T1  - Niskoenergetske nanoemulzije kao nosači za etarska ulja u topikalnim formulacijama za antioksidantnu zaštitu kože
VL  - 76
IS  - 1
SP  - 29
EP  - 42
DO  - 10.2298/HEMIND210509004G
ER  - 

@article{
author = "Gledović, Ana and Bajuk-Bogdanović, Danica and Uskoković-Marković, Snežana and Pavun, Leposava and Savić, Snežana and Janošević-Ležaić, Aleksandra",
year = "2022",
abstract = "In this study several essential oils (EOs): basil - BA, lemon balm - LB and oregano - OR were incorporated into nanoemulsions (NEs) as prospective carriers for natural and sensitive bioactives. NEs were prepared via the phase inversion composition (PIC) method, which is an energy-efficient cold process. Physicochemical stability of NEs was confirmed by particle size distribution analysis, electrical conductivity and pH value measurements, as well as by optical microscopy observations. The type of EO and the surfactant and oil mix concentration were found to be crucial factors governing the NE properties and stability. Raman spectra of the EOs confirmed main active ingredients and provided detection of interactions with the nanocarrier, which is a novel application of this technique. The antioxidant activity towards DPPH radical in methanol was concentration-dependent with a similar trend for individual oils and oil-loaded NEs (OR> LB> BA). However, the ABTS test in an aqueous medium revealed notable change in the order of activity after EO nanonisation at higher EO concentrations. Overall, it was found that OR-NE was the most effective and stable system, since OR acted as a co-stabiliser in the NE formulation, and its remarkably high antioxidant activity was successfully preserved during 6 months of storage., U ovoj studiji nekoliko različitih etarskih ulja (EU): bosiljak – BO, matičnjak – MA i origano – OR inkor-
porirano je u nanoemulzije (NE) kao potencijalne nosače za prirodne i osetljive bioaktivne sastojke. NE
su pripremljene pomoću metode inverzije faza (engl. phase inversion composition – PIC method) koja
predstavlja niskoenergetski i ekonomičan postupak izrade. Fizičkohemijska stabilnost nanoemulzija
potvrđena je analizom raspodele veličina kapi, merenjem električne provodljivosti i pH vrednosti, kao i
optičkom mikroskopijom. Nađeno je da su vrsta EU i koncentracija smeše surfaktanata i uljane faze dva
ključna faktora koja su uticala na karakteristike i stabilnost dobijenih emulzija. Tehnikom Ramanske
spektroskopije potvrđeni su glavni aktivni sastojci etarskih ulja i detektovane su moguće interakcije sa
nanonosačem, što predstavlja noviju primenu pomenute tehnike. Pokazano je da antioksidantna
aktivnost prema DPPH radikalu u metanolu zavisi od koncentracije, sa sličnim trendom za čista etarska
ulja i za nanoemulzije sa uljima (OR> MA> BO). Međutim, ABTS test u vodenoj sredini pokazao je izrazite
promene u redosledu aktivnosti sa povećanjem koncentracije EU i nakon nanonizacije EU. Generalno,
dokazano je da je OR-NE najefikasniji i najstabiliniji sistem, s obzirom da je ulje origana ispoljilo ulogu
kostabilizatora u formulaciji, uz istovremeno očuvanje njegove veoma visoke antioksidantne aktivnosti
u obliku nanoemulzije, tokom 6 meseci čuvanja.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Low energy nanoemulsions as carriers for essential oils in topical formulations for antioxidant skin protection, Niskoenergetske nanoemulzije kao nosači za etarska ulja u topikalnim formulacijama za antioksidantnu zaštitu kože",
volume = "76",
number = "1",
pages = "29-42",
doi = "10.2298/HEMIND210509004G"
}

Gledović, A., Bajuk-Bogdanović, D., Uskoković-Marković, S., Pavun, L., Savić, S.,& Janošević-Ležaić, A.. (2022). Low energy nanoemulsions as carriers for essential oils in topical formulations for antioxidant skin protection. in Hemijska industrija
Association of the Chemical Engineers of Serbia., 76(1), 29-42.
https://doi.org/10.2298/HEMIND210509004G

Gledović A, Bajuk-Bogdanović D, Uskoković-Marković S, Pavun L, Savić S, Janošević-Ležaić A. Low energy nanoemulsions as carriers for essential oils in topical formulations for antioxidant skin protection. in Hemijska industrija. 2022;76(1):29-42.
doi:10.2298/HEMIND210509004G .

Gledović, Ana, Bajuk-Bogdanović, Danica, Uskoković-Marković, Snežana, Pavun, Leposava, Savić, Snežana, Janošević-Ležaić, Aleksandra, "Low energy nanoemulsions as carriers for essential oils in topical formulations for antioxidant skin protection" in Hemijska industrija, 76, no. 1 (2022):29-42,
https://doi.org/10.2298/HEMIND210509004G . .

TY  - JOUR
AU  - Janošević-Ležaić, Aleksandra
AU  - Pašti, Igor
AU  - Gledović, Ana
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Uskoković-Marković, Snežana
AU  - Ćirić-Marjanović, Gordana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4067
AB  - Novel electroactive PANI-GA micro/nanostructured functional copolymers/co-oligomers were synthesized by the oxidative copolymerization of aniline and gallic acid (GA) in an aqueous solution, using ammonium peroxydisulfate (APS) as an oxidant and different initial mole ratios of GA to aniline ([GA]/[aniline]=0.1, 0.25, 0.5 and 1.0). It was found that the yield, molecular structure, morphology, electrical conductivity, electrochemical behavior, antioxidant and antimicrobial properties of PANI-GA strongly depend on [GA]/[aniline] ratio. The highest conductivity (3.8 × 10–3 S cm􀀀 1) showed PANI-GA with dominant polyaniline (PANI) emeraldine salt segments and nanorod morphology, synthesized at [GA]/[aniline] = 0.1. FTIR spectroscopy confirmed the presence of covalently bonded GA and PANI-type structural segments in PANI-GA. The antioxidant activity of PANI-GA was explored by the spectrophotometric 2,2′ -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay and electrochemical test for superoxide anion radical, •O2􀀀 . All protonated PANI-GA showed good scavenging activity toward ABTS•+ and •O2􀀀 radicals, whereby the highest activity exhibited PANI-GA produced at [GA]/[aniline] = 1. The radical scavenging activity of protonated PANI-GA samples toward ABTS•+ was much higher than that of PANI. The antimicrobial properties of the PANI-GA against bacteria S. aureus and E. coli, as well as against fungus C. albicans were evaluated. All synthesized protonated PANI-GA were more effective as S. aureus growth inhibitors than pristine GA and PANI. Highest effectiveness against E. coli and C. albicans, higher than that of pristine GA and PANI, showed conductive PANI-GA synthesized at [GA]/[aniline]= 0.1. Based on the obtained experimental results the mechanism of GA and aniline oxidative copolymerization is proposed.
PB  - Elsevier Ltd
T2  - Synthetic Metals
T1  - Copolymerization of aniline and gallic acid: Novel electroactive materials with antioxidant and antimicrobial activities
VL  - 286
DO  - 10.1016/j.synthmet.2022.117048
ER  - 

@article{
author = "Janošević-Ležaić, Aleksandra and Pašti, Igor and Gledović, Ana and Antić-Stanković, Jelena and Božić, Dragana and Uskoković-Marković, Snežana and Ćirić-Marjanović, Gordana",
year = "2022",
abstract = "Novel electroactive PANI-GA micro/nanostructured functional copolymers/co-oligomers were synthesized by the oxidative copolymerization of aniline and gallic acid (GA) in an aqueous solution, using ammonium peroxydisulfate (APS) as an oxidant and different initial mole ratios of GA to aniline ([GA]/[aniline]=0.1, 0.25, 0.5 and 1.0). It was found that the yield, molecular structure, morphology, electrical conductivity, electrochemical behavior, antioxidant and antimicrobial properties of PANI-GA strongly depend on [GA]/[aniline] ratio. The highest conductivity (3.8 × 10–3 S cm􀀀 1) showed PANI-GA with dominant polyaniline (PANI) emeraldine salt segments and nanorod morphology, synthesized at [GA]/[aniline] = 0.1. FTIR spectroscopy confirmed the presence of covalently bonded GA and PANI-type structural segments in PANI-GA. The antioxidant activity of PANI-GA was explored by the spectrophotometric 2,2′ -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay and electrochemical test for superoxide anion radical, •O2􀀀 . All protonated PANI-GA showed good scavenging activity toward ABTS•+ and •O2􀀀 radicals, whereby the highest activity exhibited PANI-GA produced at [GA]/[aniline] = 1. The radical scavenging activity of protonated PANI-GA samples toward ABTS•+ was much higher than that of PANI. The antimicrobial properties of the PANI-GA against bacteria S. aureus and E. coli, as well as against fungus C. albicans were evaluated. All synthesized protonated PANI-GA were more effective as S. aureus growth inhibitors than pristine GA and PANI. Highest effectiveness against E. coli and C. albicans, higher than that of pristine GA and PANI, showed conductive PANI-GA synthesized at [GA]/[aniline]= 0.1. Based on the obtained experimental results the mechanism of GA and aniline oxidative copolymerization is proposed.",
publisher = "Elsevier Ltd",
journal = "Synthetic Metals",
title = "Copolymerization of aniline and gallic acid: Novel electroactive materials with antioxidant and antimicrobial activities",
volume = "286",
doi = "10.1016/j.synthmet.2022.117048"
}

Janošević-Ležaić, A., Pašti, I., Gledović, A., Antić-Stanković, J., Božić, D., Uskoković-Marković, S.,& Ćirić-Marjanović, G.. (2022). Copolymerization of aniline and gallic acid: Novel electroactive materials with antioxidant and antimicrobial activities. in Synthetic Metals
Elsevier Ltd., 286.
https://doi.org/10.1016/j.synthmet.2022.117048

Janošević-Ležaić A, Pašti I, Gledović A, Antić-Stanković J, Božić D, Uskoković-Marković S, Ćirić-Marjanović G. Copolymerization of aniline and gallic acid: Novel electroactive materials with antioxidant and antimicrobial activities. in Synthetic Metals. 2022;286.
doi:10.1016/j.synthmet.2022.117048 .

Janošević-Ležaić, Aleksandra, Pašti, Igor, Gledović, Ana, Antić-Stanković, Jelena, Božić, Dragana, Uskoković-Marković, Snežana, Ćirić-Marjanović, Gordana, "Copolymerization of aniline and gallic acid: Novel electroactive materials with antioxidant and antimicrobial activities" in Synthetic Metals, 286 (2022),
https://doi.org/10.1016/j.synthmet.2022.117048 . .

TY  - CONF
AU  - Gledović, Ana
AU  - Bajuk-Bogdanović, Danica
AU  - Uskoković-Marković, Snežana
AU  - Pavun, Leposava
AU  - Savić, Snežana
AU  - Janošević-Ležaić, Aleksandra
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4673
AB  - Aromatic plants have been used since ancient times as a source of essential oils (EOs)
which are known as natural remedies in traditional medicine systems – phytotherapy and
aromatherapy, but they are also widely used for everyday purposes (for example, as fragrant
components in skincare preparations and perfumery; as flavours and natural preservatives
in food industry). It is known that many EOs contain molecules with considerable
antioxidant activity, such as phenolic compounds, flavonoids and terpenoids, acting alone or
synergistically. Besides antimicrobial action, EOs prepared from plants form the Lamiaceae
family, such as basil −BA (Ocimum basilicum), oregano – OR (Origanum vulgare) and lemon
balm − LB (Melissa officinalis) leaves are reported to scavenge free radicals (1). Therefore,
these particular EOs and their bioactives could be good candidates for skin-protective
formulations. Nanoemulsions (NEs), especially those produced by low energy methods, are
recently proposed as prospective carriers for sensitive and natural ingredients such as EOs,
in order to ensure their stabilization. Additionally, some EOs can act as cosurfactants and
promote low energy nanoemulsification. Having in mind that EOs cannot be used undiluted,
oil-in-water NEs could be an appropriate delivery system, representing fluid formulations
with ultra-fine droplets, pleasant visual appearance, and improved stability compared to
classic macroemulsions (2). Firstly, NEs were prepared different EOs (BA, OR or LB) with
polyethylene glycol free (PEG-free) natural surfactants/emollients mixture (containing
polyglycerol-4 laurate as the main surfactant) suitable for cold processing via the Phase
inversion composition (PIC) method. NEs physicochemical stability was confirmed by
particle size distribution analysis, electrical conductivity and pH value measurements, as
well as by optical microscopy observations. The type of EO and the concentration of
surfactants/emollient mixture were proven to be crucial factors governing NE properties
and stability. Raman spectra of EOs verified their main active ingredients and detected
possible interactions with the nanocarrier (3). The antioxidant activity towards the DPPH
radical in methanol was concentration-dependent with a similar trend for neat oils and oil-
loaded NEs (OR> LB> BA). However, the ABTS test in an aqueous medium revealed notable
change in the order of activity with increased EO concentration and after EO nanonisation.
Overall, the highest antioxidant activity was observed for OR and OR-loaded NE (> 95%
INH ABTS and > 85% INH DPPH), implying at some optimal package of OR within the nanocarrier
which preserved its high antioxidant performance and ensured its stability during 6 months
of storage.
AB  - Aromatične biljke koriste se od davnina za dobijanje etarskih ulja (EOs) koja su
poznata kao prirodni lekovi u tradicionalnim sistemima lečenja – fitoterapiji i aromaterapiji,
a takođe je široko rasprostranjena njihova upotreba u svakodnevnom životu (na primer, kao
mirisne komponente u kozmetičkim proizvodima i parfemima; kao korigensi ukusa i
prirodni konzervansi u prehrambenoj industriji). Brojna EOs sadrže molekule kao što su
fenolna jedinjenja, flavonoidi i terpenoidi koji ispoljavaju značajnu antioksidativnu aktivnost,
delujući samostalno ili sinergistički. Pored antimikrobnog dejstva, EOs dobijena iz listova
biljaka familije Lamiaceae, kao što je bosiljak - BA (Ocimum basilicum), origano – OR
(Origanum vulgare) i matičnjak − LB (Melissa officinalis) deluju kao hvatači slobodnih
radikala (1). Prema tome, pomenuta EOs i njihovi bioaktivni sastojci mogu biti dobri
kandidati za razvoj formulacija za zaštitu kože. Nanoemulzije (NEs), posebno one
proizvedene niskoenergetskim metodama, odnedavno se predlažu kao prospektivni nosači
za prirodne i osetljive sastojke poput EOs, sa ciljem da se obezbedi njihova stabilizacija.
Dodatno, neka EOs imaju ulogu kosurfaktanta i pospešuju niskoenergetsku
nanoemulzifikaciju. Imajući u vidu da se EOs ne mogu koristiti nerazblažena, NEs ulje u vodi
tipa mogu biti adekvatni nosači, s obzirom da su u pitanju fluidne formulacije sa ultra-finim
veličinama kapi, prijatnim vizuelnim izgledom, kao i poboljšanom stabilnošću u poređenju sa
klasičnim makroemulzijama (2). Prvo su pripremljene NEs sa različitim EOs (BA, OR ili LB),
sa smešom prirodnim surfaktanata i emolijensa (bez polietilenglikola - PEG, gde je
poligliceril-4 laurat glavni surfaktant) koja je pogodna za izradu na hladno primenom
metode inverzije faza (engl. Phase inversion composition ‐ PIC method). Fizičkohemijska
stabilnost NEs potvrđena je analizom distribucije veličina kapi, merenjem električne
provodljivosti i pH vrednosti, kao i optičkom mikroskopijom. Nađeno je da su vrsta EO i
koncentracija smeše surfaktanata i emolijensa ključni faktori koji utiču na karakteristike i
stabilnost dobijenih NE. Tehnikom Ramanske spektroskopije potvrđeni su glavni aktivni
sastojci etarskih ulja i detektovane su moguće interakcije sa nanonosačem. Pokazano je da je
antioksidativna aktivnost prema DPPH radikalu u metanolu koncentraciono zavisna, sa
sličnim trendom za čista etarska ulja i za NEs sa uljima (OR> LB> BA). Međutim, ABTS test u
vodenoj sredini pokazao je izrazite promene u redosledu aktivnosti sa povećanjem
koncentracije EO i nakon nanonizacije EO. Generalno, najjača aktioksidativna aktivnost
primećena je u slučaju OR i OR-NE (> 95% INH ABTS i > 85% INH DPPH), što ukazuje na
optimalno pakovanje OR unutar nanonosača čime su očuvane njegove antioksidativne
performanse i obezbeđena stabilnost tokom 6 meseci čuvanja.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Low energy nanoemulsions as potential carriers for essential oils in formulations for antioxidant skin protection
T1  - Niskoenergetske nanoemulzije kao potencijalni nosači za etarska ulja u formulacijama za antioksidativnu zaštitu kože
VL  - 71
IS  - 5 suplement
SP  - S96
EP  - S99
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4673
ER  - 

@conference{
author = "Gledović, Ana and Bajuk-Bogdanović, Danica and Uskoković-Marković, Snežana and Pavun, Leposava and Savić, Snežana and Janošević-Ležaić, Aleksandra",
year = "2021",
abstract = "Aromatic plants have been used since ancient times as a source of essential oils (EOs)
which are known as natural remedies in traditional medicine systems – phytotherapy and
aromatherapy, but they are also widely used for everyday purposes (for example, as fragrant
components in skincare preparations and perfumery; as flavours and natural preservatives
in food industry). It is known that many EOs contain molecules with considerable
antioxidant activity, such as phenolic compounds, flavonoids and terpenoids, acting alone or
synergistically. Besides antimicrobial action, EOs prepared from plants form the Lamiaceae
family, such as basil −BA (Ocimum basilicum), oregano – OR (Origanum vulgare) and lemon
balm − LB (Melissa officinalis) leaves are reported to scavenge free radicals (1). Therefore,
these particular EOs and their bioactives could be good candidates for skin-protective
formulations. Nanoemulsions (NEs), especially those produced by low energy methods, are
recently proposed as prospective carriers for sensitive and natural ingredients such as EOs,
in order to ensure their stabilization. Additionally, some EOs can act as cosurfactants and
promote low energy nanoemulsification. Having in mind that EOs cannot be used undiluted,
oil-in-water NEs could be an appropriate delivery system, representing fluid formulations
with ultra-fine droplets, pleasant visual appearance, and improved stability compared to
classic macroemulsions (2). Firstly, NEs were prepared different EOs (BA, OR or LB) with
polyethylene glycol free (PEG-free) natural surfactants/emollients mixture (containing
polyglycerol-4 laurate as the main surfactant) suitable for cold processing via the Phase
inversion composition (PIC) method. NEs physicochemical stability was confirmed by
particle size distribution analysis, electrical conductivity and pH value measurements, as
well as by optical microscopy observations. The type of EO and the concentration of
surfactants/emollient mixture were proven to be crucial factors governing NE properties
and stability. Raman spectra of EOs verified their main active ingredients and detected
possible interactions with the nanocarrier (3). The antioxidant activity towards the DPPH
radical in methanol was concentration-dependent with a similar trend for neat oils and oil-
loaded NEs (OR> LB> BA). However, the ABTS test in an aqueous medium revealed notable
change in the order of activity with increased EO concentration and after EO nanonisation.
Overall, the highest antioxidant activity was observed for OR and OR-loaded NE (> 95%
INH ABTS and > 85% INH DPPH), implying at some optimal package of OR within the nanocarrier
which preserved its high antioxidant performance and ensured its stability during 6 months
of storage., Aromatične biljke koriste se od davnina za dobijanje etarskih ulja (EOs) koja su
poznata kao prirodni lekovi u tradicionalnim sistemima lečenja – fitoterapiji i aromaterapiji,
a takođe je široko rasprostranjena njihova upotreba u svakodnevnom životu (na primer, kao
mirisne komponente u kozmetičkim proizvodima i parfemima; kao korigensi ukusa i
prirodni konzervansi u prehrambenoj industriji). Brojna EOs sadrže molekule kao što su
fenolna jedinjenja, flavonoidi i terpenoidi koji ispoljavaju značajnu antioksidativnu aktivnost,
delujući samostalno ili sinergistički. Pored antimikrobnog dejstva, EOs dobijena iz listova
biljaka familije Lamiaceae, kao što je bosiljak - BA (Ocimum basilicum), origano – OR
(Origanum vulgare) i matičnjak − LB (Melissa officinalis) deluju kao hvatači slobodnih
radikala (1). Prema tome, pomenuta EOs i njihovi bioaktivni sastojci mogu biti dobri
kandidati za razvoj formulacija za zaštitu kože. Nanoemulzije (NEs), posebno one
proizvedene niskoenergetskim metodama, odnedavno se predlažu kao prospektivni nosači
za prirodne i osetljive sastojke poput EOs, sa ciljem da se obezbedi njihova stabilizacija.
Dodatno, neka EOs imaju ulogu kosurfaktanta i pospešuju niskoenergetsku
nanoemulzifikaciju. Imajući u vidu da se EOs ne mogu koristiti nerazblažena, NEs ulje u vodi
tipa mogu biti adekvatni nosači, s obzirom da su u pitanju fluidne formulacije sa ultra-finim
veličinama kapi, prijatnim vizuelnim izgledom, kao i poboljšanom stabilnošću u poređenju sa
klasičnim makroemulzijama (2). Prvo su pripremljene NEs sa različitim EOs (BA, OR ili LB),
sa smešom prirodnim surfaktanata i emolijensa (bez polietilenglikola - PEG, gde je
poligliceril-4 laurat glavni surfaktant) koja je pogodna za izradu na hladno primenom
metode inverzije faza (engl. Phase inversion composition ‐ PIC method). Fizičkohemijska
stabilnost NEs potvrđena je analizom distribucije veličina kapi, merenjem električne
provodljivosti i pH vrednosti, kao i optičkom mikroskopijom. Nađeno je da su vrsta EO i
koncentracija smeše surfaktanata i emolijensa ključni faktori koji utiču na karakteristike i
stabilnost dobijenih NE. Tehnikom Ramanske spektroskopije potvrđeni su glavni aktivni
sastojci etarskih ulja i detektovane su moguće interakcije sa nanonosačem. Pokazano je da je
antioksidativna aktivnost prema DPPH radikalu u metanolu koncentraciono zavisna, sa
sličnim trendom za čista etarska ulja i za NEs sa uljima (OR> LB> BA). Međutim, ABTS test u
vodenoj sredini pokazao je izrazite promene u redosledu aktivnosti sa povećanjem
koncentracije EO i nakon nanonizacije EO. Generalno, najjača aktioksidativna aktivnost
primećena je u slučaju OR i OR-NE (> 95% INH ABTS i > 85% INH DPPH), što ukazuje na
optimalno pakovanje OR unutar nanonosača čime su očuvane njegove antioksidativne
performanse i obezbeđena stabilnost tokom 6 meseci čuvanja.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Low energy nanoemulsions as potential carriers for essential oils in formulations for antioxidant skin protection, Niskoenergetske nanoemulzije kao potencijalni nosači za etarska ulja u formulacijama za antioksidativnu zaštitu kože",
volume = "71",
number = "5 suplement",
pages = "S96-S99",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4673"
}

Gledović, A., Bajuk-Bogdanović, D., Uskoković-Marković, S., Pavun, L., Savić, S.,& Janošević-Ležaić, A.. (2021). Low energy nanoemulsions as potential carriers for essential oils in formulations for antioxidant skin protection. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 71(5 suplement), S96-S99.
https://hdl.handle.net/21.15107/rcub_farfar_4673

Gledović A, Bajuk-Bogdanović D, Uskoković-Marković S, Pavun L, Savić S, Janošević-Ležaić A. Low energy nanoemulsions as potential carriers for essential oils in formulations for antioxidant skin protection. in Arhiv za farmaciju. 2021;71(5 suplement):S96-S99.
https://hdl.handle.net/21.15107/rcub_farfar_4673 .

Gledović, Ana, Bajuk-Bogdanović, Danica, Uskoković-Marković, Snežana, Pavun, Leposava, Savić, Snežana, Janošević-Ležaić, Aleksandra, "Low energy nanoemulsions as potential carriers for essential oils in formulations for antioxidant skin protection" in Arhiv za farmaciju, 71, no. 5 suplement (2021):S96-S99,
https://hdl.handle.net/21.15107/rcub_farfar_4673 .

TY  - JOUR
AU  - Gledović, Ana
AU  - Janošević-Ležaić, Aleksandra
AU  - Nikolić, Ines
AU  - Tasić-Kostov, Marija
AU  - Antić-Stanković, Jelena
AU  - Krstonošić, Veljko
AU  - Ranđelović, Danijela
AU  - Božić, Dragana
AU  - Ilić, Dušan
AU  - Tamburić, Slobodanka
AU  - Savić, Snežana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3777
AB  - This study focuses on the development of biocompatible oil-in-water (O/W) nanoemulsions based on polyglycerol esters, as promising carriers for natural actives: red raspberry seed oil—RO and hydro-glycolic fruit extracts from red raspberry—RE and French oak—FE. Nanoemulsions were obtained via phase inversion composition (PIC) method at room temperature by dilution of microemulsion phase, confirmed by visual appearance, percentage of transmittance, microscopic, rheological and Differential Scanning Calorimetry (DSC) investigations. The results have shown that the basic RO-loaded formulation could be further enriched with hydro-glycolic fruit extracts from red raspberry or French oak, while keeping a semi-transparent appearance due to the fine droplet size (Z-ave: 50 to 70 nm, PDI value ≤ 0.1). The highest antioxidant activity (~92% inhibition of the DPPH radical) was achieved in the formulation containing both lipophilic (RO) and hydrophilic antioxidants (FE), due to their synergistic effect. The nanoemulsion carrier significantly increased the selective cytotoxic effect of RO towards malignant melanoma (Fem-X) cells, compared to normal human keratinocytes (HaCaT). In vivo study on human volunteers showed satisfactory safety profiles and significant improvement in skin hydration during 2 h after application for all nanoemulsions. Therefore, polyglycerol ester-based nanoemulsions can be promoted as effective carriers for red raspberry seed oil and/or hydro-glycolic fruit extracts in topical formulations intended for skin protection and hydration.
PB  - MDPI AG
T2  - Nanomaterials
T1  - Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance
VL  - 11
IS  - 1
SP  - 1
EP  - 21
DO  - 10.3390/nano11010217
ER  - 

@article{
author = "Gledović, Ana and Janošević-Ležaić, Aleksandra and Nikolić, Ines and Tasić-Kostov, Marija and Antić-Stanković, Jelena and Krstonošić, Veljko and Ranđelović, Danijela and Božić, Dragana and Ilić, Dušan and Tamburić, Slobodanka and Savić, Snežana",
year = "2021",
abstract = "This study focuses on the development of biocompatible oil-in-water (O/W) nanoemulsions based on polyglycerol esters, as promising carriers for natural actives: red raspberry seed oil—RO and hydro-glycolic fruit extracts from red raspberry—RE and French oak—FE. Nanoemulsions were obtained via phase inversion composition (PIC) method at room temperature by dilution of microemulsion phase, confirmed by visual appearance, percentage of transmittance, microscopic, rheological and Differential Scanning Calorimetry (DSC) investigations. The results have shown that the basic RO-loaded formulation could be further enriched with hydro-glycolic fruit extracts from red raspberry or French oak, while keeping a semi-transparent appearance due to the fine droplet size (Z-ave: 50 to 70 nm, PDI value ≤ 0.1). The highest antioxidant activity (~92% inhibition of the DPPH radical) was achieved in the formulation containing both lipophilic (RO) and hydrophilic antioxidants (FE), due to their synergistic effect. The nanoemulsion carrier significantly increased the selective cytotoxic effect of RO towards malignant melanoma (Fem-X) cells, compared to normal human keratinocytes (HaCaT). In vivo study on human volunteers showed satisfactory safety profiles and significant improvement in skin hydration during 2 h after application for all nanoemulsions. Therefore, polyglycerol ester-based nanoemulsions can be promoted as effective carriers for red raspberry seed oil and/or hydro-glycolic fruit extracts in topical formulations intended for skin protection and hydration.",
publisher = "MDPI AG",
journal = "Nanomaterials",
title = "Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance",
volume = "11",
number = "1",
pages = "1-21",
doi = "10.3390/nano11010217"
}

Gledović, A., Janošević-Ležaić, A., Nikolić, I., Tasić-Kostov, M., Antić-Stanković, J., Krstonošić, V., Ranđelović, D., Božić, D., Ilić, D., Tamburić, S.,& Savić, S.. (2021). Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance. in Nanomaterials
MDPI AG., 11(1), 1-21.
https://doi.org/10.3390/nano11010217

Gledović A, Janošević-Ležaić A, Nikolić I, Tasić-Kostov M, Antić-Stanković J, Krstonošić V, Ranđelović D, Božić D, Ilić D, Tamburić S, Savić S. Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance. in Nanomaterials. 2021;11(1):1-21.
doi:10.3390/nano11010217 .

Gledović, Ana, Janošević-Ležaić, Aleksandra, Nikolić, Ines, Tasić-Kostov, Marija, Antić-Stanković, Jelena, Krstonošić, Veljko, Ranđelović, Danijela, Božić, Dragana, Ilić, Dušan, Tamburić, Slobodanka, Savić, Snežana, "Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance" in Nanomaterials, 11, no. 1 (2021):1-21,
https://doi.org/10.3390/nano11010217 . .

TY  - JOUR
AU  - Gledović, Ana
AU  - Janošević-Ležaić, Aleksandra
AU  - Krstonošić, Veljko
AU  - Đoković, Jelena
AU  - Nikolić, Ines
AU  - Bajuk-Bogdanović, Danica
AU  - Antić-Stanković, Jelena
AU  - Ranđelović, Danijela
AU  - Savić, Sanela M.
AU  - Filipović, Mila
AU  - Tamburić, Slobodanka
AU  - Savić, Snežana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3581
AB  - Considering a growing demand for medicinal/cosmetic products with natural actives, this study focuses on the low-energy nanoemulsions (LE-NEs) prepared via the Phase inversion composition (PIC) method at room temperature as potential carriers for natural oil. Four different red raspberry seed oils (ROs) were tested, as follows: cold-pressed vs. CO2- extracted, organic vs. non-organic, refined vs. unrefined. The oil phase was optimized with Tocopheryl acetate and Isostearyl isostearate, while water phase was adjusted with either glycerol or an antioxidant hydro-glycolic extract. This study has used a combined approach to formulation development, employing both conventional methods (pseudo-ternary phase diagram - PTPD, electrical conductivity, particle size measurements, microscopical analysis, and rheological measurements) and the methods novel to this area, such as textural analysis and Raman spectroscopy. Raman spectroscopy has detected fine differences in chemical composition among ROs, and it detected the interactions within nanoemulsions. It was shown that the cold-pressed, unrefined, organic grade oil (RO2) with 6.62% saturated fatty acids and 92.25% unsaturated fatty acids, was optimal for the LE-NEs. Textural analysis confirmed the existence of cubic gel-like phase as a crucial step in the formation of stable RO2-loaded LE-NEs, with droplets in the narrow nano-range (125 to 135 nm; PDI ≤ 0.1). The DPPH test in methanol and ABTS in aqueous medium have revealed a synergistic free radical scavenging effect between lipophilic and hydrophilic antioxidants in LE-NEs. The nanoemulsion carrier has improved the biological effect of raw materials on HeLa cervical adenocarcinoma cells, while exhibiting good safety profile, as confirmed on MRC-5 normal human lung fibroblasts. Overall, this study has shown that low-energy nanoemulsions present very promising carriers for topical delivery of natural bioactives. Raman spectroscopy and textural analysis have proven to be a useful addition to the arsenal of methods used in the formulation and characterization of nanoemulsion systems.
PB  - Public Library of Science
T2  - PLoS ONE
T1  - Low-energy nanoemulsions as carriers for red raspberry seed oil: Formulation approach based on Raman spectroscopy and textural analysis, physicochemical properties, stability and in vitro antioxidant/ biological activity
VL  - 15
IS  - 4
DO  - 10.1371/journal.pone.0230993
ER  - 

@article{
author = "Gledović, Ana and Janošević-Ležaić, Aleksandra and Krstonošić, Veljko and Đoković, Jelena and Nikolić, Ines and Bajuk-Bogdanović, Danica and Antić-Stanković, Jelena and Ranđelović, Danijela and Savić, Sanela M. and Filipović, Mila and Tamburić, Slobodanka and Savić, Snežana",
year = "2020",
abstract = "Considering a growing demand for medicinal/cosmetic products with natural actives, this study focuses on the low-energy nanoemulsions (LE-NEs) prepared via the Phase inversion composition (PIC) method at room temperature as potential carriers for natural oil. Four different red raspberry seed oils (ROs) were tested, as follows: cold-pressed vs. CO2- extracted, organic vs. non-organic, refined vs. unrefined. The oil phase was optimized with Tocopheryl acetate and Isostearyl isostearate, while water phase was adjusted with either glycerol or an antioxidant hydro-glycolic extract. This study has used a combined approach to formulation development, employing both conventional methods (pseudo-ternary phase diagram - PTPD, electrical conductivity, particle size measurements, microscopical analysis, and rheological measurements) and the methods novel to this area, such as textural analysis and Raman spectroscopy. Raman spectroscopy has detected fine differences in chemical composition among ROs, and it detected the interactions within nanoemulsions. It was shown that the cold-pressed, unrefined, organic grade oil (RO2) with 6.62% saturated fatty acids and 92.25% unsaturated fatty acids, was optimal for the LE-NEs. Textural analysis confirmed the existence of cubic gel-like phase as a crucial step in the formation of stable RO2-loaded LE-NEs, with droplets in the narrow nano-range (125 to 135 nm; PDI ≤ 0.1). The DPPH test in methanol and ABTS in aqueous medium have revealed a synergistic free radical scavenging effect between lipophilic and hydrophilic antioxidants in LE-NEs. The nanoemulsion carrier has improved the biological effect of raw materials on HeLa cervical adenocarcinoma cells, while exhibiting good safety profile, as confirmed on MRC-5 normal human lung fibroblasts. Overall, this study has shown that low-energy nanoemulsions present very promising carriers for topical delivery of natural bioactives. Raman spectroscopy and textural analysis have proven to be a useful addition to the arsenal of methods used in the formulation and characterization of nanoemulsion systems.",
publisher = "Public Library of Science",
journal = "PLoS ONE",
title = "Low-energy nanoemulsions as carriers for red raspberry seed oil: Formulation approach based on Raman spectroscopy and textural analysis, physicochemical properties, stability and in vitro antioxidant/ biological activity",
volume = "15",
number = "4",
doi = "10.1371/journal.pone.0230993"
}

Gledović, A., Janošević-Ležaić, A., Krstonošić, V., Đoković, J., Nikolić, I., Bajuk-Bogdanović, D., Antić-Stanković, J., Ranđelović, D., Savić, S. M., Filipović, M., Tamburić, S.,& Savić, S.. (2020). Low-energy nanoemulsions as carriers for red raspberry seed oil: Formulation approach based on Raman spectroscopy and textural analysis, physicochemical properties, stability and in vitro antioxidant/ biological activity. in PLoS ONE
Public Library of Science., 15(4).
https://doi.org/10.1371/journal.pone.0230993

Gledović A, Janošević-Ležaić A, Krstonošić V, Đoković J, Nikolić I, Bajuk-Bogdanović D, Antić-Stanković J, Ranđelović D, Savić SM, Filipović M, Tamburić S, Savić S. Low-energy nanoemulsions as carriers for red raspberry seed oil: Formulation approach based on Raman spectroscopy and textural analysis, physicochemical properties, stability and in vitro antioxidant/ biological activity. in PLoS ONE. 2020;15(4).
doi:10.1371/journal.pone.0230993 .

Gledović, Ana, Janošević-Ležaić, Aleksandra, Krstonošić, Veljko, Đoković, Jelena, Nikolić, Ines, Bajuk-Bogdanović, Danica, Antić-Stanković, Jelena, Ranđelović, Danijela, Savić, Sanela M., Filipović, Mila, Tamburić, Slobodanka, Savić, Snežana, "Low-energy nanoemulsions as carriers for red raspberry seed oil: Formulation approach based on Raman spectroscopy and textural analysis, physicochemical properties, stability and in vitro antioxidant/ biological activity" in PLoS ONE, 15, no. 4 (2020),
https://doi.org/10.1371/journal.pone.0230993 . .

TY  - JOUR
AU  - Filipović, Mila
AU  - Gledović, Ana
AU  - Lukić, Milica
AU  - Tasić-Kostov, Marija
AU  - Isailović, Tanja
AU  - Pantelić, Ivana
AU  - Vuleta, Gordana
AU  - Savić, Snežana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2628
AB  - Background/Aim. Since skin moisturization may be achieved by both actives and chosen carrier, plant stem cells, squalene and natural alkyl polyglucoside emulsifier may be potential components of contemporary cosmetic products. The aim of the study was in vivo evaluation of the skin irritation potential and the efficacy of Alpine Rose stem cells incorporated into liposomes and olive oil squalene as ingredients of moisturizing creams, with respect to the novel emulsifier used for creams' stabilization. Methods. With the employment of noninvasive skin biophysical measurements, skin hydration (EC), transepidermal water loss (TEWL), erythema index (EI) and viscoelasticity were measured on 76 healthy volunteers. In the first phase, skin irritation after a 24-hour occlusion and the long-term efficacy of creams (a 21-day study) on healthy skin were evaluated. Phase II of the study focused on the cream efficacy assessment after a 6-day treatment of sodium lauryl sulfate-irritated skin. Results. After a 24-hour occlusion, there were no significant changes in the EI for any tested sample. In the second phase of the study, the EI was not significantly altered for the cream containing squalene, while the application of all active samples resulted in a significant reduction of TEWL. In both phases of the study an EC increase was recorded, especially for the squalene-containing cream. Conclusion. Due to the lack of skin irritation and skin barrier impairment along with the marked hydration effect, it could be said that the investigated actives incorporated into alkyl polyglucoside emulsifier-stabilized creams may be safely applied as ingredients for "tailor-made" cosmetic moisturizers intended for normal and dry skin care, whereas olive oil squalene could be used for the treatment of irritated or sensitive skin as well.
AB  - Uvod/Cilj. S obzirom na to da vlaženje kože može biti postignuto izborom kako aktivnih supstanci, tako i odgovarajućeg nosača, biljne matične ćelije, skvalen i prirodni alkil poliglukozidni emulgator mogu biti potencijalni sastojci savremenih kozmetičkih proizvoda. Cilj ovog rada bio je in vivo procena iritiranog potencijala i efikasnosti matičnih ćelija alpske ruže dodatih u liposome i skvalena maslinovog ulja, kao sastojaka vlažećih krema, imajući u vidu nov emulgator koji je korišćen za njihovu stabilizaciju. Metode. Upotrebom neinvazivnih metoda zasnovanih na biofizičkim merenjima na koži, kod 76 zdravih dobrovoljaca mereni su: hidratacija kože (EC), transepidermalni gubitak vlage (TEWL), eritema indeks (EI) i viskoelastičnost kože. U prvoj fazi studije vršena je procena iritacije kože nakon 24-h okluzije, kao i procena efikasnosti krema nakon primene na zdravoj koži dobrovoljaca u trajanju od 21 dana. Druga faza studije bila je bazirana na proceni efikasnosti krema nakon 6-dnevnog tretmana kože prethodno iritirane natrijum-lauril-sulfatom. Rezultati. Nakon 24-h okluzije nije bilo značajne promene vrednosti EI ni kod jednog ispitivanog krema. U drugoj fazi studije, krem sa skvalenom nije značajno uticao na promenu vrednosti EI, dok je primena svih aktivnih krema dovela do značajnog sniženja vrednosti TEWL. U obe faze studije zabeležen je porast EC, naročito nakon primene krema koji sadrži skvalen. Zaključak. Uzevši u obzir odsustvo nadraženosti kože i narušavanja kožne barijere, kao i porast hidratacije površinskog sloja kože, može se reći da se ispitivane aktivne supstance ubačene u kreme stabilizovane alkil-poliglukozidnim emulgatorom mogu bezbedno koristiti kao komponente tzv. "skrojenih" kozmetičkih ovlaživača namenjenih za negu zdrave i suve kože, dok se skvalen maslinovog ulja može koristiti i za negu iritirane i osetljive kože.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Alp Rose stem cells, olive oil squalene and a natural alkyl polyglucoside emulsifier: Are they appropriate ingredients of skin moisturizers - in vivo efficiency on normal and sodium lauryl sulfate-irritated skin?
T1  - Matične ćelije alpske ruže, skvalen maslinovog ulja i prirodni alkil-poliglukozidni emulgator - da li su odgovarajući sastojci kremova za vlaženje - in vivo efikasnost na zdravoj i koži iritiranoj natrijum lauril sulfatom?
VL  - 73
IS  - 11
SP  - 991
EP  - 1002
DO  - 10.2298/VSP150116122F
ER  - 

@article{
author = "Filipović, Mila and Gledović, Ana and Lukić, Milica and Tasić-Kostov, Marija and Isailović, Tanja and Pantelić, Ivana and Vuleta, Gordana and Savić, Snežana",
year = "2016",
abstract = "Background/Aim. Since skin moisturization may be achieved by both actives and chosen carrier, plant stem cells, squalene and natural alkyl polyglucoside emulsifier may be potential components of contemporary cosmetic products. The aim of the study was in vivo evaluation of the skin irritation potential and the efficacy of Alpine Rose stem cells incorporated into liposomes and olive oil squalene as ingredients of moisturizing creams, with respect to the novel emulsifier used for creams' stabilization. Methods. With the employment of noninvasive skin biophysical measurements, skin hydration (EC), transepidermal water loss (TEWL), erythema index (EI) and viscoelasticity were measured on 76 healthy volunteers. In the first phase, skin irritation after a 24-hour occlusion and the long-term efficacy of creams (a 21-day study) on healthy skin were evaluated. Phase II of the study focused on the cream efficacy assessment after a 6-day treatment of sodium lauryl sulfate-irritated skin. Results. After a 24-hour occlusion, there were no significant changes in the EI for any tested sample. In the second phase of the study, the EI was not significantly altered for the cream containing squalene, while the application of all active samples resulted in a significant reduction of TEWL. In both phases of the study an EC increase was recorded, especially for the squalene-containing cream. Conclusion. Due to the lack of skin irritation and skin barrier impairment along with the marked hydration effect, it could be said that the investigated actives incorporated into alkyl polyglucoside emulsifier-stabilized creams may be safely applied as ingredients for "tailor-made" cosmetic moisturizers intended for normal and dry skin care, whereas olive oil squalene could be used for the treatment of irritated or sensitive skin as well., Uvod/Cilj. S obzirom na to da vlaženje kože može biti postignuto izborom kako aktivnih supstanci, tako i odgovarajućeg nosača, biljne matične ćelije, skvalen i prirodni alkil poliglukozidni emulgator mogu biti potencijalni sastojci savremenih kozmetičkih proizvoda. Cilj ovog rada bio je in vivo procena iritiranog potencijala i efikasnosti matičnih ćelija alpske ruže dodatih u liposome i skvalena maslinovog ulja, kao sastojaka vlažećih krema, imajući u vidu nov emulgator koji je korišćen za njihovu stabilizaciju. Metode. Upotrebom neinvazivnih metoda zasnovanih na biofizičkim merenjima na koži, kod 76 zdravih dobrovoljaca mereni su: hidratacija kože (EC), transepidermalni gubitak vlage (TEWL), eritema indeks (EI) i viskoelastičnost kože. U prvoj fazi studije vršena je procena iritacije kože nakon 24-h okluzije, kao i procena efikasnosti krema nakon primene na zdravoj koži dobrovoljaca u trajanju od 21 dana. Druga faza studije bila je bazirana na proceni efikasnosti krema nakon 6-dnevnog tretmana kože prethodno iritirane natrijum-lauril-sulfatom. Rezultati. Nakon 24-h okluzije nije bilo značajne promene vrednosti EI ni kod jednog ispitivanog krema. U drugoj fazi studije, krem sa skvalenom nije značajno uticao na promenu vrednosti EI, dok je primena svih aktivnih krema dovela do značajnog sniženja vrednosti TEWL. U obe faze studije zabeležen je porast EC, naročito nakon primene krema koji sadrži skvalen. Zaključak. Uzevši u obzir odsustvo nadraženosti kože i narušavanja kožne barijere, kao i porast hidratacije površinskog sloja kože, može se reći da se ispitivane aktivne supstance ubačene u kreme stabilizovane alkil-poliglukozidnim emulgatorom mogu bezbedno koristiti kao komponente tzv. "skrojenih" kozmetičkih ovlaživača namenjenih za negu zdrave i suve kože, dok se skvalen maslinovog ulja može koristiti i za negu iritirane i osetljive kože.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Alp Rose stem cells, olive oil squalene and a natural alkyl polyglucoside emulsifier: Are they appropriate ingredients of skin moisturizers - in vivo efficiency on normal and sodium lauryl sulfate-irritated skin?, Matične ćelije alpske ruže, skvalen maslinovog ulja i prirodni alkil-poliglukozidni emulgator - da li su odgovarajući sastojci kremova za vlaženje - in vivo efikasnost na zdravoj i koži iritiranoj natrijum lauril sulfatom?",
volume = "73",
number = "11",
pages = "991-1002",
doi = "10.2298/VSP150116122F"
}

Filipović, M., Gledović, A., Lukić, M., Tasić-Kostov, M., Isailović, T., Pantelić, I., Vuleta, G.,& Savić, S.. (2016). Alp Rose stem cells, olive oil squalene and a natural alkyl polyglucoside emulsifier: Are they appropriate ingredients of skin moisturizers - in vivo efficiency on normal and sodium lauryl sulfate-irritated skin?. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 73(11), 991-1002.
https://doi.org/10.2298/VSP150116122F

Filipović M, Gledović A, Lukić M, Tasić-Kostov M, Isailović T, Pantelić I, Vuleta G, Savić S. Alp Rose stem cells, olive oil squalene and a natural alkyl polyglucoside emulsifier: Are they appropriate ingredients of skin moisturizers - in vivo efficiency on normal and sodium lauryl sulfate-irritated skin?. in Vojnosanitetski pregled. 2016;73(11):991-1002.
doi:10.2298/VSP150116122F .

Filipović, Mila, Gledović, Ana, Lukić, Milica, Tasić-Kostov, Marija, Isailović, Tanja, Pantelić, Ivana, Vuleta, Gordana, Savić, Snežana, "Alp Rose stem cells, olive oil squalene and a natural alkyl polyglucoside emulsifier: Are they appropriate ingredients of skin moisturizers - in vivo efficiency on normal and sodium lauryl sulfate-irritated skin?" in Vojnosanitetski pregled, 73, no. 11 (2016):991-1002,
https://doi.org/10.2298/VSP150116122F . .

TY  - JOUR
AU  - Filipović, Mila
AU  - Lukić, Milica
AU  - Krstonosić, Veljko
AU  - Đorđević, Sanela
AU  - Pantelić, Ivana
AU  - Gledović, Ana
AU  - Vuleta, Gordana
AU  - Savić, Snežana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2527
AB  - During the formulation of liposome-containing products different problems can occur and the selection of a suitable carrier remains the greatest challenge. To estimate feasibility of a novel alkyl polyglucoside surfactant (hydroxystearyl alcohol and hydroxystearyl glucoside) as an emulsifier for cosmetics with liposome-encapsulated plant stem cells, we performed a two-phase study. In the first, the pre-formulation phase, the emulsifier's critical micelle concentration (CMC) and liposome-encapsulated active-emulsifier interactions were determined. The second phase was carried out to develop and characterize a cosmetic emulsion suitable to serve as a carrier for liposomes. The investigated emulsifier, with the obtained CMC value of 0.0085 wt.%, could be classified as liposome-friendly and can be used to develop stable and aesthetically acceptable cosmetics or even prospective pharmaceutical liposome-containing emulsions.
PB  - Carl Hanser Verlag, Munich
T2  - Tenside Surfactants Detergents
T1  - Feasibility of a Natural Surfactant as a Stabilizer for Cosmetics with Liposome-Encapsulated Plant Stem Cells: Pre-Formulation and Formulation Through Stability Studies
VL  - 53
IS  - 3
SP  - 214
EP  - 226
DO  - 10.3139/113.110426
ER  - 

@article{
author = "Filipović, Mila and Lukić, Milica and Krstonosić, Veljko and Đorđević, Sanela and Pantelić, Ivana and Gledović, Ana and Vuleta, Gordana and Savić, Snežana",
year = "2016",
abstract = "During the formulation of liposome-containing products different problems can occur and the selection of a suitable carrier remains the greatest challenge. To estimate feasibility of a novel alkyl polyglucoside surfactant (hydroxystearyl alcohol and hydroxystearyl glucoside) as an emulsifier for cosmetics with liposome-encapsulated plant stem cells, we performed a two-phase study. In the first, the pre-formulation phase, the emulsifier's critical micelle concentration (CMC) and liposome-encapsulated active-emulsifier interactions were determined. The second phase was carried out to develop and characterize a cosmetic emulsion suitable to serve as a carrier for liposomes. The investigated emulsifier, with the obtained CMC value of 0.0085 wt.%, could be classified as liposome-friendly and can be used to develop stable and aesthetically acceptable cosmetics or even prospective pharmaceutical liposome-containing emulsions.",
publisher = "Carl Hanser Verlag, Munich",
journal = "Tenside Surfactants Detergents",
title = "Feasibility of a Natural Surfactant as a Stabilizer for Cosmetics with Liposome-Encapsulated Plant Stem Cells: Pre-Formulation and Formulation Through Stability Studies",
volume = "53",
number = "3",
pages = "214-226",
doi = "10.3139/113.110426"
}

Filipović, M., Lukić, M., Krstonosić, V., Đorđević, S., Pantelić, I., Gledović, A., Vuleta, G.,& Savić, S.. (2016). Feasibility of a Natural Surfactant as a Stabilizer for Cosmetics with Liposome-Encapsulated Plant Stem Cells: Pre-Formulation and Formulation Through Stability Studies. in Tenside Surfactants Detergents
Carl Hanser Verlag, Munich., 53(3), 214-226.
https://doi.org/10.3139/113.110426

Filipović M, Lukić M, Krstonosić V, Đorđević S, Pantelić I, Gledović A, Vuleta G, Savić S. Feasibility of a Natural Surfactant as a Stabilizer for Cosmetics with Liposome-Encapsulated Plant Stem Cells: Pre-Formulation and Formulation Through Stability Studies. in Tenside Surfactants Detergents. 2016;53(3):214-226.
doi:10.3139/113.110426 .

Filipović, Mila, Lukić, Milica, Krstonosić, Veljko, Đorđević, Sanela, Pantelić, Ivana, Gledović, Ana, Vuleta, Gordana, Savić, Snežana, "Feasibility of a Natural Surfactant as a Stabilizer for Cosmetics with Liposome-Encapsulated Plant Stem Cells: Pre-Formulation and Formulation Through Stability Studies" in Tenside Surfactants Detergents, 53, no. 3 (2016):214-226,
https://doi.org/10.3139/113.110426 . .