@conference{
author = "Kuzmanović Nedeljković, Snežana and Ćujić Nikolić, Nada and Stević, Tatjana and Bigović, Dubravka and Šavikin, Katarina and Filipić, Brankica",
year = "2024",
abstract = "Antimicrobial resistance (AMR) threatens to in- crease its mortality rate to 10 million deaths per year by 2050, and Acinetobacter bauman- nii stands out with its insensitivity to almost all available therapeutic options. The risk factors for the acquisition of A. baumannii infections have become more frequent during the coronavirus disease 2019 (COVID-19) pandemic, consequent- ly leading to increased AMR. Potential solutions may be explored among naturally derived prod- ucts, where essential oils (EOs) stand out with a broad spectrum of antimicrobial activity, com- plex chemical composition, and non-specific mechanisms of action postponing AMR devel- opment. However, their volatile nature may af- fect stability during shelf-life. Thus, we aimed to develop stable and effective microencapsulat- ed systems of essential oils (EOs) derived from two species from Lamiaceae family, Origanum heracleoticum L. (oregano) and Thymus vulgar- is L. (thyme). Microencapsulated complexes of oregano and thyme EOs (OEOC and TEOC) were prepared with hydroxypropyl-β-cyclodextrin as a carrier, and freeze-dried. Fourier-transform infrared spectroscopy verified the formation of inclusion complexes, while thermal stability was confirmed by differential scanning calorimetry. The microdilution broth assay revealed higher antimicrobial activity of the OEOC sample com- pared to TEOC against 64 A. baumannii isolates recovered from COVID-19 patients admitted to intensive care units (MIC values 0.4–1.6 mg/mL for OEOC, and ≥ 1.76 mg/mL for TEOC). Sub-in- hibitory concentrations of microencapsulated EOs significantly decreased the biofilm forma- tion of four A. baumannii isolates, representatives of a group of isolates based on the genetic pat- tern (Isolates 1, 2, 39, and 54). Concerning Isolate 2 (representing 60 of the total 64 isolates), the re- duction was achieved for more than 50% by both samples. Contrary to antimicrobial potential, TEOC displayed slightly better antioxidant activ- ity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. These results highlight the potential of mi- croencapsulated oregano and thyme EOs in the treatment of infections caused by A. baumannii.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia with international participation, Mikromed regio 5: From biotechnology to human and planetary health, UMS series 24: 4th – 6th april 2024, Belgrade, Serbia, Book of Abstracts",
title = "Microencapsulated essential oils from two Lamiaceae species for combat against multi-resistant Acinetobacter baumannii",
pages = "179-179",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5689"
}