@conference{
author = "Otašević, Biljana and Svrkota, Bojana and Krmar, Jovana and Protić, Ana and Zečević, Mira",
year = "2022",
abstract = "Liquid chromatography which implies that an analyte interacts through several
separation mechanisms (modes) with a stationary phase packed in a single chromatographic
column is called multimodal or mixed-mode chromatography (MMC). Based on the combined
modes, MMC is seen as bimodal (RP/HILIC, RP/IEX, HILIC/IEX) or trimodal (different
RP/HILIC/IEXcombinations) system. Consequently, compounds that encompass wide
spectra of properties (nonpolar, polar, organic, inorganic, ionized and/or non-ionized) can
be chromatographed in a single chromatographic run. The main practical achievement of this
is the reduction of the number of required analyses needed per one complex sample
compared to unimodal chromatographic systems. Therefore, the popularity of MMC grows
rapidly in recent years together with the number of its applications (1). Beside common
quality control issues that include active pharmaceutical ingredients and related substances
analysis and impurity profiling, the range of different analytes which MMC successfully
handles extends to the analyses of drugs in environmental and biological samples, peptides
and proteins. Since nearly half of recently FDA approved pharmaceutical substances are in
the form of a salt, the focus of MMC turned to pharmaceutical counterions analyses as well
(2). However, separations are governed by numerous intermolecular interactions resulting
from specific analyteʼs properties (size, charge, polarity) and mobile phase composition
(aqueous phase ionic strength and pH value, organic solvent content) while the quality of
separation can also be affected by column temperature and mobile phase flow rate.
Eventually, analytical method development is challenging and demands the assistance of
multifactorial optimization strategies such as the design of experiments., Tečna hromatografija koja podrazumeva da analit interaguje putem nekoliko
mehanizama razdvajanja (modova) sa stacionarnom fazom upakovanom u jednu istu
hromatografsku kolonu naziva se multimodalna hromatografija (MMC). Na osnovu
kombinovanih modova, MMC se posmatra kao bimodalni (RP/HILIC, RP/IEX, HILIC/IEX) ili
trimodalni (različite RP/HILIC/IEX kombinacije) sistem. Ovo za posledicu ima da jedinjenja
širokog spektra svojstava (nepolarna, polarna, organska, neorganska, jonizovana i/ili
nejonizovana) mogu se hromatografisati u jednom ciklusu hromatografije. Glavni praktični
doprinos ovoga je smanjenje broja potrebnih analiza po jednom složenom uzorku u
poređenju sa unimodalnim hromatografskim sistemima. Zbog toga, popularnost MMC naglo
raste poslednjih godina zajedno sa brojem njenih aplikacija (1). Pored uobičajenih pitanja
kontrole kvaliteta koja uključuju analizu aktivnih farmaceutskih sastojaka i srodnih
supstanci i profilisanje nečistoća, opseg različitih analita sa kojima MMC uspešno pokriva
proširen je analitikom lekova iz prirodnog okruženja i bioloških uzoraka, peptidima i
proteinima. Pošto je skoro polovina farmaceutskih supstanci koje je nedavno FDA odobrila u
obliku soli, fokus MMC je orjentisan i ka analizi farmaceutskih kontrajona (2). Međutim,
hromatografsko razdvajanje je vođeno brojnim intermolekularnim interakcijima koje su
rezultat specifičnih svojstava analita (veličina, naelektrisanje, polaritet) i mobilne faze
(jonska jačina i pH vrednost vodene faze, sadržaj organskog rastvarača), dok na kvalitet
razdvajanja može uticati i temperatura kolone i brzina protoka mobilne faze. Na kraju, razvoj
analitičkih metoda predstavlja izazov i zahteva podršku u strategijama multifaktorske
optimizacije kao što je dizajn eksperimenata.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "The use of multimodal chromatography in the control of pharmaceutical products: New possibilities and new challenges, Primena multimodalne hromatografije u kontroli farmaceutskih proizvoda: Nove mogućnosti i novi izazovi",
volume = "72",
number = "suppl. 4",
pages = "57-58",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4688"
}