TY  - JOUR
AU  - Mirković, Duško
AU  - Beletić, Anđelo
AU  - Savić, Miroslav
AU  - Milinković, Neda
AU  - Sarić-Matutinović, Marija
AU  - Jančić, Ivan
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4910
AB  - Occupational and environmental toxicology specialists find catecholamine fluctuations in brain tissue relevant for research of neurotoxicity, such as that induced by manganese or zinc, pesticides, industrial solvents, plastic, air pollution, or irradiation. Considering that catecholamine tissue concentrations are generally very low, their extraction requires a reliable and optimal method that will achieve maximum recovery and minimise other interferences. This study aimed to evaluate whether the aluminium (III) oxide (Al2O3, alumina) based cartridges designed for catecholamine isolation from plasma could be used for solid-phase extraction (SPE) of catecholamine from the brain tissue. To do that, we homogenised Wistar rat brain tissue with perchloric acid and compared three extraction techniques: SPE, the routine filtration through a 0.22 µm membrane filter, and their combination. In the extracts, we compared relative chromatographic catecholamine mobility measured with high performance liquid chromatography with electrochemical detection. Chromatographic patterns for norepinephrine and epinephrine were similar regardless of the extraction technique, which indicates that the alumina cartridge is good enough to isolate them from brain tissue. However, the dopamine pattern was unsatisfactory, and further experiments are needed to identify the issue and optimise the protocol. © 2023 Duško Mirković et al., published by Sciendo.  Promjene razine katekolamina (KAT) u moždanom tkivu značajne su za brojna područja profesionalne toksikologije odnosno ekotoksikologije u kojima se istražuje neurotoksičnost izazvana različitim agensima poput mangana ili cinka, pesticida, industrijskih otapala, plastike, aerozagađenja ili zračenja. Niske koncentracije KAT-a u tkivu zahtijevaju pouzdanu i učinkovitu tehniku ekstrakcije kojom se postiže maksimalni „prinos“ katekolamina i minimalni sadržaj interferirajućih spojeva. Cilj istraživanja bio je procijeniti mogu li se kolone na bazi aluminijeva (III) oksida (Al2O3), dizajnirane za izolaciju KAT-a iz plazme, koristiti za ekstrakciju čvrstom fazom (eng. solid-phase extraction – SPE) KAT-a iz moždanoga tkiva. Nakon homogenizacije tkiva Sprague Dawley štakora upotrebom perklorne kiseline, primijenjene su tri tehnike ekstrakcije: SPE, filtracija kroz 0,22 µm membranski filtar, koji je zapravo rutinska tehnika za izolaciju KAT-a iz mozga, i kombinacija tih dviju tehnika. U ekstraktima je relativna kromatografska pokretljivost KAT-a analizirana HPLC metodom s elektrokemijskom detekcijom. Ponašanje norepinefrina i epinefrina tijekom kromatografije bilo je slično, bez obzira na tehniku ekstrakcije, što upućuje na to da aluminijev oksid ima zadovoljavajuća svojstva izolirati ta dva KAT-a iz moždanoga tkiva. Međutim, uočeni su problemi s ekstrakcijom dopamina, koji zahtijevaju dodatne eksperimente kako bi se otkrio njihov uzrok i osmislio protokol optimizacije. Author keywords aluminijev oksid; aluminium oxide; brain; catecholamines; ekstrakcija čvrste faze; katekolamini; mozak; solid phase extraction; tissue; tkivo   © This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine      1of1   Top of page Cited by 0 documents Inform me when this document is cited in Scopus: Related documents Find more related documents in Scopus based on: Authors Keywords
AB  - Promjene razine katekolamina (KAT) u moždanom tkivu značajne su za brojna područja profesionalne toksikologije odnosno ekotoksikologije u kojima se istražuje neurotoksičnost izazvana različitim agensima poput mangana ili cinka, pesticida, industrijskih otapala, plastike, aerozagađenja ili zračenja. Niske koncentracije KAT-a u tkivu zahtijevaju pouzdanu i učinkovitu tehniku ekstrakcije kojom se postiže maksimalni „prinos“ katekolamina i minimalni sadržaj interferirajućih spojeva. Cilj istraživanja bio je procijeniti mogu li se kolone na bazi aluminijeva (III) oksida (Al2 O 3), dizajnirane za izolaciju KAT-a iz plazme, koristiti za ekstrakciju čvrstom fazom (eng. solid-phase extraction – SPE) KAT-a iz moždanoga tkiva. Nakon homogenizacije tkiva Sprague Dawley štakora upotrebom perklorne kiseline, primijenjene su tri tehnike ekstrakcije: SPE, filtracija kroz 0,22 μm membranski filtar, koji je zapravo rutinska tehnika za izolaciju KAT-a iz mozga, i kombinacija tih dviju tehnika. U ekstraktima je relativna kromatografska pokretljivost KAT-a analizirana HPLC metodom s elektrokemijskom detekcijom. Ponašanje norepinefrina i epinefrina tijekom kromatografije bilo je slično, bez obzira na tehniku ekstrakcije, što upućuje na to da aluminijev oksid ima zadovoljavajuća svojstva izolirati ta dva KAT-a iz moždanoga tkiva. Međutim, uočeni su problemi s ekstrakcijom dopamina, koji zahtijevaju dodatne eksperimente kako bi se otkrio njihov uzrok i osmislio protokol optimizacije.
PB  - De Gruyter Poland
T2  - Arhiv za higijenu rada i toksikologiju
T1  - Is alumina suitable for solid phase extraction of catecholamines from brain tissue?
T1  - Je li aluminijev oksid pogodan za postupak ekstrakcije čvrste faze katekolamina iz moždanoga tkiva?
VL  - 74
IS  - 2
SP  - 120
EP  - 126
DO  - 10.2478/aiht-2023-74-3706
ER  - 

@article{
author = "Mirković, Duško and Beletić, Anđelo and Savić, Miroslav and Milinković, Neda and Sarić-Matutinović, Marija and Jančić, Ivan",
year = "2023",
abstract = "Occupational and environmental toxicology specialists find catecholamine fluctuations in brain tissue relevant for research of neurotoxicity, such as that induced by manganese or zinc, pesticides, industrial solvents, plastic, air pollution, or irradiation. Considering that catecholamine tissue concentrations are generally very low, their extraction requires a reliable and optimal method that will achieve maximum recovery and minimise other interferences. This study aimed to evaluate whether the aluminium (III) oxide (Al2O3, alumina) based cartridges designed for catecholamine isolation from plasma could be used for solid-phase extraction (SPE) of catecholamine from the brain tissue. To do that, we homogenised Wistar rat brain tissue with perchloric acid and compared three extraction techniques: SPE, the routine filtration through a 0.22 µm membrane filter, and their combination. In the extracts, we compared relative chromatographic catecholamine mobility measured with high performance liquid chromatography with electrochemical detection. Chromatographic patterns for norepinephrine and epinephrine were similar regardless of the extraction technique, which indicates that the alumina cartridge is good enough to isolate them from brain tissue. However, the dopamine pattern was unsatisfactory, and further experiments are needed to identify the issue and optimise the protocol. © 2023 Duško Mirković et al., published by Sciendo.  Promjene razine katekolamina (KAT) u moždanom tkivu značajne su za brojna područja profesionalne toksikologije odnosno ekotoksikologije u kojima se istražuje neurotoksičnost izazvana različitim agensima poput mangana ili cinka, pesticida, industrijskih otapala, plastike, aerozagađenja ili zračenja. Niske koncentracije KAT-a u tkivu zahtijevaju pouzdanu i učinkovitu tehniku ekstrakcije kojom se postiže maksimalni „prinos“ katekolamina i minimalni sadržaj interferirajućih spojeva. Cilj istraživanja bio je procijeniti mogu li se kolone na bazi aluminijeva (III) oksida (Al2O3), dizajnirane za izolaciju KAT-a iz plazme, koristiti za ekstrakciju čvrstom fazom (eng. solid-phase extraction – SPE) KAT-a iz moždanoga tkiva. Nakon homogenizacije tkiva Sprague Dawley štakora upotrebom perklorne kiseline, primijenjene su tri tehnike ekstrakcije: SPE, filtracija kroz 0,22 µm membranski filtar, koji je zapravo rutinska tehnika za izolaciju KAT-a iz mozga, i kombinacija tih dviju tehnika. U ekstraktima je relativna kromatografska pokretljivost KAT-a analizirana HPLC metodom s elektrokemijskom detekcijom. Ponašanje norepinefrina i epinefrina tijekom kromatografije bilo je slično, bez obzira na tehniku ekstrakcije, što upućuje na to da aluminijev oksid ima zadovoljavajuća svojstva izolirati ta dva KAT-a iz moždanoga tkiva. Međutim, uočeni su problemi s ekstrakcijom dopamina, koji zahtijevaju dodatne eksperimente kako bi se otkrio njihov uzrok i osmislio protokol optimizacije. Author keywords aluminijev oksid; aluminium oxide; brain; catecholamines; ekstrakcija čvrste faze; katekolamini; mozak; solid phase extraction; tissue; tkivo   © This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine      1of1   Top of page Cited by 0 documents Inform me when this document is cited in Scopus: Related documents Find more related documents in Scopus based on: Authors Keywords, Promjene razine katekolamina (KAT) u moždanom tkivu značajne su za brojna područja profesionalne toksikologije odnosno ekotoksikologije u kojima se istražuje neurotoksičnost izazvana različitim agensima poput mangana ili cinka, pesticida, industrijskih otapala, plastike, aerozagađenja ili zračenja. Niske koncentracije KAT-a u tkivu zahtijevaju pouzdanu i učinkovitu tehniku ekstrakcije kojom se postiže maksimalni „prinos“ katekolamina i minimalni sadržaj interferirajućih spojeva. Cilj istraživanja bio je procijeniti mogu li se kolone na bazi aluminijeva (III) oksida (Al2 O 3), dizajnirane za izolaciju KAT-a iz plazme, koristiti za ekstrakciju čvrstom fazom (eng. solid-phase extraction – SPE) KAT-a iz moždanoga tkiva. Nakon homogenizacije tkiva Sprague Dawley štakora upotrebom perklorne kiseline, primijenjene su tri tehnike ekstrakcije: SPE, filtracija kroz 0,22 μm membranski filtar, koji je zapravo rutinska tehnika za izolaciju KAT-a iz mozga, i kombinacija tih dviju tehnika. U ekstraktima je relativna kromatografska pokretljivost KAT-a analizirana HPLC metodom s elektrokemijskom detekcijom. Ponašanje norepinefrina i epinefrina tijekom kromatografije bilo je slično, bez obzira na tehniku ekstrakcije, što upućuje na to da aluminijev oksid ima zadovoljavajuća svojstva izolirati ta dva KAT-a iz moždanoga tkiva. Međutim, uočeni su problemi s ekstrakcijom dopamina, koji zahtijevaju dodatne eksperimente kako bi se otkrio njihov uzrok i osmislio protokol optimizacije.",
publisher = "De Gruyter Poland",
journal = "Arhiv za higijenu rada i toksikologiju",
title = "Is alumina suitable for solid phase extraction of catecholamines from brain tissue?, Je li aluminijev oksid pogodan za postupak ekstrakcije čvrste faze katekolamina iz moždanoga tkiva?",
volume = "74",
number = "2",
pages = "120-126",
doi = "10.2478/aiht-2023-74-3706"
}

Mirković, D., Beletić, A., Savić, M., Milinković, N., Sarić-Matutinović, M.,& Jančić, I.. (2023). Is alumina suitable for solid phase extraction of catecholamines from brain tissue?. in Arhiv za higijenu rada i toksikologiju
De Gruyter Poland., 74(2), 120-126.
https://doi.org/10.2478/aiht-2023-74-3706

Mirković D, Beletić A, Savić M, Milinković N, Sarić-Matutinović M, Jančić I. Is alumina suitable for solid phase extraction of catecholamines from brain tissue?. in Arhiv za higijenu rada i toksikologiju. 2023;74(2):120-126.
doi:10.2478/aiht-2023-74-3706 .

Mirković, Duško, Beletić, Anđelo, Savić, Miroslav, Milinković, Neda, Sarić-Matutinović, Marija, Jančić, Ivan, "Is alumina suitable for solid phase extraction of catecholamines from brain tissue?" in Arhiv za higijenu rada i toksikologiju, 74, no. 2 (2023):120-126,
https://doi.org/10.2478/aiht-2023-74-3706 . .

TY  - JOUR
AU  - Bećarević, Mirjana
AU  - Jovičić, Snežana
AU  - Ignjatović, Svetlana
AU  - Mirković, Duško
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4320
AB  - Background: The investigation of the prevalence of the IgG and the IgM isotypes of anticardiolipin (aCL) and antib2glycoprotein I (ab2gpI) Abs in healthy Serbian middle-aged subjects was the main goal of our study. In addition, we analyzed the potential associations of above-mentioned Abs with serum proteins and lipids/lipoproteins. Methods: Forty healthy subjects were included in our study. Obesity (BMI 30 kg/m2) was present in 8/40 (20%) subjects. Titers of analyzed Abs were measured by ELISA. Results: The prevalence of IgG and IgM ab2gpI Abs was 5% and 12.5%, respectively, while the prevalence of IgM aCL was 10%. The IgG ab2gpI Abs were significantly different between subjects with normal triglycerides levels and those with hypertriglyceridemia (Mann-Whitney, P = 0.014). The significant difference in hsCRP concentrations was observed between subjects with the increased levels of the IgM isotype of aCL Abs and those with normal IgM aCL values (Mann-Whitney, P = 0.028). Conclusions: Dyslipidemia and BMI ≥30 were associated with aPL Abs and therefore, the correction of BMI and lipid status might be beneficial in reduction or elimination of predisposing factors that might trigger thrombotic events in otherwise healthy middle-aged subjects. Larger national study is necessary to confirm our findings.
AB  - Uvod: Analiza prevalentnosti IgG i IgM izotipa antikardiolip- inskih (aCL) i anti- b2glikoprotein I (ab2gpI) At kod zdravih sredove~nih stanovnika Srbije je bila glavni cilj na{e studije. Dodatno, analizirali smo potencijalnu povezanost gore- navedenih At sa serumskim proteinima i lipidima/lipopro- teinima. Metode: 40 zdravih ispitanika je bilo uklju~eno u na{u studiju. Gojaznost (BMI ≥ 30 kg/m2) je uo~ena kod 8/40 (20%) osoba. Titri analiziranih antitela su utvr|ivani ELISA testom. Rezultati: Prevalentnost IgG i IgM ab2gpIAt je bila 5% i 12.5%, redom, dok je prevalentnost IgM aCL bila 10%. Nivoi IgG ab2gpI At su se zna~ajno razlikovali izme|u ispi- tanika sa i bez hipertrigliceridemije (Mann-Whitney, P = 0.014). Zna~ajne razlike u hsCRP koncentracijama uo~ene su izme|u osoba sa povi{enim nivoima IgM aCL At i onih sa referentim vrednostima (Mann-Whitney, P = 0,028). Zaklju~ak: Dislipidemija i BMI ≥30 su bili povezani sa aPL At uprkos njihovoj niskoj prevalentnosti, i zato korekcija BMI i lipidnog statusa bi bila korisna u redukciji ili elimi- naciji predispoziraju}ih faktora koji mogu da izazovu trom- boti~ki doga|aj kod ina~e zdravih sredove~nih ispitanika. Obimnije nacionalne studije su neophodne da bi potvrdile na{e nalaze.
PB  - Society of Medical Biochemists of Serbia
T2  - Journal of Medical Biochemistry
T1  - Antiphospholipid antibodies in healthy serbian middle-aged subjects: preliminary data
T1  - Antifosfolipidna antitela u zdravih srpskih osoba srednjih godina - preliminarni podaci
VL  - 41
IS  - 4
SP  - 506
EP  - 512
DO  - 10.5937/JOMB0-35642
ER  - 

@article{
author = "Bećarević, Mirjana and Jovičić, Snežana and Ignjatović, Svetlana and Mirković, Duško",
year = "2022",
abstract = "Background: The investigation of the prevalence of the IgG and the IgM isotypes of anticardiolipin (aCL) and antib2glycoprotein I (ab2gpI) Abs in healthy Serbian middle-aged subjects was the main goal of our study. In addition, we analyzed the potential associations of above-mentioned Abs with serum proteins and lipids/lipoproteins. Methods: Forty healthy subjects were included in our study. Obesity (BMI 30 kg/m2) was present in 8/40 (20%) subjects. Titers of analyzed Abs were measured by ELISA. Results: The prevalence of IgG and IgM ab2gpI Abs was 5% and 12.5%, respectively, while the prevalence of IgM aCL was 10%. The IgG ab2gpI Abs were significantly different between subjects with normal triglycerides levels and those with hypertriglyceridemia (Mann-Whitney, P = 0.014). The significant difference in hsCRP concentrations was observed between subjects with the increased levels of the IgM isotype of aCL Abs and those with normal IgM aCL values (Mann-Whitney, P = 0.028). Conclusions: Dyslipidemia and BMI ≥30 were associated with aPL Abs and therefore, the correction of BMI and lipid status might be beneficial in reduction or elimination of predisposing factors that might trigger thrombotic events in otherwise healthy middle-aged subjects. Larger national study is necessary to confirm our findings., Uvod: Analiza prevalentnosti IgG i IgM izotipa antikardiolip- inskih (aCL) i anti- b2glikoprotein I (ab2gpI) At kod zdravih sredove~nih stanovnika Srbije je bila glavni cilj na{e studije. Dodatno, analizirali smo potencijalnu povezanost gore- navedenih At sa serumskim proteinima i lipidima/lipopro- teinima. Metode: 40 zdravih ispitanika je bilo uklju~eno u na{u studiju. Gojaznost (BMI ≥ 30 kg/m2) je uo~ena kod 8/40 (20%) osoba. Titri analiziranih antitela su utvr|ivani ELISA testom. Rezultati: Prevalentnost IgG i IgM ab2gpIAt je bila 5% i 12.5%, redom, dok je prevalentnost IgM aCL bila 10%. Nivoi IgG ab2gpI At su se zna~ajno razlikovali izme|u ispi- tanika sa i bez hipertrigliceridemije (Mann-Whitney, P = 0.014). Zna~ajne razlike u hsCRP koncentracijama uo~ene su izme|u osoba sa povi{enim nivoima IgM aCL At i onih sa referentim vrednostima (Mann-Whitney, P = 0,028). Zaklju~ak: Dislipidemija i BMI ≥30 su bili povezani sa aPL At uprkos njihovoj niskoj prevalentnosti, i zato korekcija BMI i lipidnog statusa bi bila korisna u redukciji ili elimi- naciji predispoziraju}ih faktora koji mogu da izazovu trom- boti~ki doga|aj kod ina~e zdravih sredove~nih ispitanika. Obimnije nacionalne studije su neophodne da bi potvrdile na{e nalaze.",
publisher = "Society of Medical Biochemists of Serbia",
journal = "Journal of Medical Biochemistry",
title = "Antiphospholipid antibodies in healthy serbian middle-aged subjects: preliminary data, Antifosfolipidna antitela u zdravih srpskih osoba srednjih godina - preliminarni podaci",
volume = "41",
number = "4",
pages = "506-512",
doi = "10.5937/JOMB0-35642"
}

Bećarević, M., Jovičić, S., Ignjatović, S.,& Mirković, D.. (2022). Antiphospholipid antibodies in healthy serbian middle-aged subjects: preliminary data. in Journal of Medical Biochemistry
Society of Medical Biochemists of Serbia., 41(4), 506-512.
https://doi.org/10.5937/JOMB0-35642

Bećarević M, Jovičić S, Ignjatović S, Mirković D. Antiphospholipid antibodies in healthy serbian middle-aged subjects: preliminary data. in Journal of Medical Biochemistry. 2022;41(4):506-512.
doi:10.5937/JOMB0-35642 .

Bećarević, Mirjana, Jovičić, Snežana, Ignjatović, Svetlana, Mirković, Duško, "Antiphospholipid antibodies in healthy serbian middle-aged subjects: preliminary data" in Journal of Medical Biochemistry, 41, no. 4 (2022):506-512,
https://doi.org/10.5937/JOMB0-35642 . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Sarić, Marija
AU  - Jovičić, Snežana
AU  - Mirković, Duško
AU  - Ležaić, Višnja
AU  - Ignjatović, Svetlana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3624
AB  - Background:Some observational studies indicate an association  of  25-hydroxy  vitamin  D  (25(OH)D)  insufficiency and  atherogenic  cholesterol  concentrations.  The  aim  of this study was to investigate relationship between 25(OH)D concentrations  and  lipid  parameters  in  end  stage  renal disease  (ESRD)  patients,  separately  for  predialysis,  hemodialysis and peritoneal dialysis patients.Methods:We  have  adjusted  25(OH)D  concentrations  for seasonal variability with cosinor analysis, and performed all further analysis using these corrected 25(OH)D concentrations. Concentrations of 25(OH)D and the lipid parameters were  determined  in  214  ESRD  patients  and  50  control group participants. The analysis included the measurement of 25(OH)D by HPLC, apolipoprotein (Apo) AI, ApoB andLp(a) by nephelometry, total cholesterol (TC), high-densitylipoprotein  cholesterol  (HDL-C),  low-density  lipoprotein cholesterol  (LDL-C)  and  triglyceride  (TG)  by  spectrophotometry   and   manually   calculated   ApoB/ApoAI   and LDL-C/HDL-C ratio.Results:ESRD patients with adjusted 25(OH)D concentrations  of  ≤ 50  nmol/L  had  significantly  higher  TC  (P  =0.005) and ApoAI (P = 0.049). Significantly higher HDL-C  (P  =  0.011)  and  ApoAI  (P  =  0.020)  were  found  in hemodialysis patients with the 25(OH)D concentrations of ≤ 50 nmol/L. The other analyzed lipid parameters differed significantly  between  predialysis,  hemodialysis  and  peritoneal dialysis patients with 25(OH)D concentrations of <50 nmol/L. Conclusions:Our study indicate the significant relationship between 25(OH)D repletion and optimal concentrations of lipid parameters in ESRD patients. Further research is necessary  to  explain  whether  joint  evaluation  of  vitamin  D status and lipid abnormalities could improve cardiovascular outcome in ESRD patients.
AB  - Uvod: Opservacione studije ukazuju na povezanost nedostatka 25-hidroksi vitamin D (25(OH)D) i koncentracija aterogenog holesterola. Cilj ove studije bio je da se ispita međusobna povezanost 25(OH)D i lipidnih parametara kod bolesnika sa krajnjim stadijumom bolesti bubrega (ESRD) posebno kod predijaliznih, bolesnika na hemodijalizi i peritonalnoj dijalizi. Metode: Podesili smo koncentracije 25(OH)D za sezonsku varijaciju koristeći kosinor analizu, i u daljem ispitivanju smo koristili korigovane koncentracije 25(OH)D. Koncentracije 25(OH)D i lipidnih parametara su određene za 214 ESRD bolesnika i 50 učesnika kontrolne grupe. Analizirali smo koncentracije 25(OH)D metodom HPLC, apolipoproteina (Apo) AI, ApoB i Lp(a) nefelometrijski, ukupnog holesterola (TC), lipoproteina velike gustine (HDL-C), lipoproteina male gustine (LDL-C) i triglicerida (TG) spektro fotometrijski i ručno preračunavali indekse ApoB/ApoAI i LDL-C/HDLC. Rezultati: ESRD bolesnici sa korigovanim 25(OH)D 50 nmol/L su imali značajno viši TC (P = 0,005) i ApoAI (P = 0,049). Značajno više koncentracije HDL-C (P = 0,011) i ApoAI (P = 0,020) smo dobili kod hemodijaliznih bolesnika sa 25(OH)D koncentracijama 50 nmol/L. Ostali analizirani lipidni parametri su bili značajno različiti između analiziranih grupa bolesnika sa 25(OH)D koncentracijama < 50 nmol/L. Zaključak: Naša studija ukazuje na značajnu povezanost dovoljnih nivoa 25(OH)D i optimalnih koncentracija lipidnih parametara kod ESRD bolesnika. Potrebna su dalja istraživanja da bi se objasnilo da li zajednička procena statusa vitamina D i lipidnih abnormalnosti mogu da poboljšaju kardiovaskularni ishod kod ESRD bolesnika.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients
T1  - Povezanost lipidnog statusa sa 25-hidroksi vitaminom D: studija preseka kod bolesnika sa krajnjim stadijumom bolesti bubrega
VL  - 39
IS  - 3
SP  - 309
EP  - 317
DO  - 10.2478/jomb-2019-0032
ER  - 

@article{
author = "Milinković, Neda and Sarić, Marija and Jovičić, Snežana and Mirković, Duško and Ležaić, Višnja and Ignjatović, Svetlana",
year = "2020",
abstract = "Background:Some observational studies indicate an association  of  25-hydroxy  vitamin  D  (25(OH)D)  insufficiency and  atherogenic  cholesterol  concentrations.  The  aim  of this study was to investigate relationship between 25(OH)D concentrations  and  lipid  parameters  in  end  stage  renal disease  (ESRD)  patients,  separately  for  predialysis,  hemodialysis and peritoneal dialysis patients.Methods:We  have  adjusted  25(OH)D  concentrations  for seasonal variability with cosinor analysis, and performed all further analysis using these corrected 25(OH)D concentrations. Concentrations of 25(OH)D and the lipid parameters were  determined  in  214  ESRD  patients  and  50  control group participants. The analysis included the measurement of 25(OH)D by HPLC, apolipoprotein (Apo) AI, ApoB andLp(a) by nephelometry, total cholesterol (TC), high-densitylipoprotein  cholesterol  (HDL-C),  low-density  lipoprotein cholesterol  (LDL-C)  and  triglyceride  (TG)  by  spectrophotometry   and   manually   calculated   ApoB/ApoAI   and LDL-C/HDL-C ratio.Results:ESRD patients with adjusted 25(OH)D concentrations  of  ≤ 50  nmol/L  had  significantly  higher  TC  (P  =0.005) and ApoAI (P = 0.049). Significantly higher HDL-C  (P  =  0.011)  and  ApoAI  (P  =  0.020)  were  found  in hemodialysis patients with the 25(OH)D concentrations of ≤ 50 nmol/L. The other analyzed lipid parameters differed significantly  between  predialysis,  hemodialysis  and  peritoneal dialysis patients with 25(OH)D concentrations of <50 nmol/L. Conclusions:Our study indicate the significant relationship between 25(OH)D repletion and optimal concentrations of lipid parameters in ESRD patients. Further research is necessary  to  explain  whether  joint  evaluation  of  vitamin  D status and lipid abnormalities could improve cardiovascular outcome in ESRD patients., Uvod: Opservacione studije ukazuju na povezanost nedostatka 25-hidroksi vitamin D (25(OH)D) i koncentracija aterogenog holesterola. Cilj ove studije bio je da se ispita međusobna povezanost 25(OH)D i lipidnih parametara kod bolesnika sa krajnjim stadijumom bolesti bubrega (ESRD) posebno kod predijaliznih, bolesnika na hemodijalizi i peritonalnoj dijalizi. Metode: Podesili smo koncentracije 25(OH)D za sezonsku varijaciju koristeći kosinor analizu, i u daljem ispitivanju smo koristili korigovane koncentracije 25(OH)D. Koncentracije 25(OH)D i lipidnih parametara su određene za 214 ESRD bolesnika i 50 učesnika kontrolne grupe. Analizirali smo koncentracije 25(OH)D metodom HPLC, apolipoproteina (Apo) AI, ApoB i Lp(a) nefelometrijski, ukupnog holesterola (TC), lipoproteina velike gustine (HDL-C), lipoproteina male gustine (LDL-C) i triglicerida (TG) spektro fotometrijski i ručno preračunavali indekse ApoB/ApoAI i LDL-C/HDLC. Rezultati: ESRD bolesnici sa korigovanim 25(OH)D 50 nmol/L su imali značajno viši TC (P = 0,005) i ApoAI (P = 0,049). Značajno više koncentracije HDL-C (P = 0,011) i ApoAI (P = 0,020) smo dobili kod hemodijaliznih bolesnika sa 25(OH)D koncentracijama 50 nmol/L. Ostali analizirani lipidni parametri su bili značajno različiti između analiziranih grupa bolesnika sa 25(OH)D koncentracijama < 50 nmol/L. Zaključak: Naša studija ukazuje na značajnu povezanost dovoljnih nivoa 25(OH)D i optimalnih koncentracija lipidnih parametara kod ESRD bolesnika. Potrebna su dalja istraživanja da bi se objasnilo da li zajednička procena statusa vitamina D i lipidnih abnormalnosti mogu da poboljšaju kardiovaskularni ishod kod ESRD bolesnika.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients, Povezanost lipidnog statusa sa 25-hidroksi vitaminom D: studija preseka kod bolesnika sa krajnjim stadijumom bolesti bubrega",
volume = "39",
number = "3",
pages = "309-317",
doi = "10.2478/jomb-2019-0032"
}

Milinković, N., Sarić, M., Jovičić, S., Mirković, D., Ležaić, V.,& Ignjatović, S.. (2020). Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 39(3), 309-317.
https://doi.org/10.2478/jomb-2019-0032

Milinković N, Sarić M, Jovičić S, Mirković D, Ležaić V, Ignjatović S. Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients. in Journal of Medical Biochemistry. 2020;39(3):309-317.
doi:10.2478/jomb-2019-0032 .

Milinković, Neda, Sarić, Marija, Jovičić, Snežana, Mirković, Duško, Ležaić, Višnja, Ignjatović, Svetlana, "Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients" in Journal of Medical Biochemistry, 39, no. 3 (2020):309-317,
https://doi.org/10.2478/jomb-2019-0032 . .

TY  - JOUR
AU  - Đukanović, Ljubica
AU  - Lezaić, Višnja
AU  - Bukvić, Danica
AU  - Mirković, Duško
AU  - Marić, Ivko
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3247
AB  - Background: A previous study indicated that Balkan endemic nephropathy (BEN) patients in the early stage of the disease had significantly higher creatinine clearance (Ccr) than healthy persons. The aim of the study was to assess whether tubular creatinine secretion affects Ccr in early stages of BEN and to check the applicability of serum creatinine-based glomerular filtration rate (GFR) equations in these patients. Methods: The study involved 21 BEN patients with estimated GFR (eGFR) above 60 mL/min/1.73 m(2), excluding any conditions that could affect GFR or tubular creatinine secretion, and 15 healthy controls. In all participants Ccr with and without cimetidine and iohexol clearance (mGFR) were measured and eGFR calculated using Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) and Modification of Diet in Renal Disease Study (MDRD) equations. Glomerular hyperfiltration cutoff (GFR-HF) was calculated. Results: There was no significant difference between the groups in Ccr before and after cimetidine or for eGFR, but mGFR was significantly higher in BEN patients than in controls (122.02 +/- 28.03 mL/min/1.73 m(2) vs. 101.15 +/- 27.32 mL/min/1.73 m(2); p = 0.032). Cimetidine administration reduced Ccr by 10% in both groups. The ratio of Ccr to mGFR was significantly above one in seven BEN patients and five controls and their mGFR values were similar. Seven other patients and eight controls had this ratio equal to one, while values below one were recorded for seven more patients and two controls. mGFR of all these 14 patients was significantly higher than that of healthy controls (129.88 +/- 27.52 mL/min/1.73 m(2) vs. 107.43 +/- 19.51 mL/min/1.73 m(2); p = 0.009). Mean GFR-HF was significantly higher than mGFR in controls, but these two values were similar in BEN patients. eGFR underestimated mGFR in both BEN patients and controls. Conclusion: The ratio of Ccr to mGFR and mGFR to GFR-HF indicated that elevated mGFR in early stages of BEN could be explained by increased glomerular filtration, but tubular creatinine secretion augmented Ccr in a smaller proportion of patients, who did not differ from healthy subjects.
PB  - MDPI, Basel
T2  - Medicina-Lithuania
T1  - Increased Glomerular Filtration Rate in Early Stage of Balkan Endemic Nephropathy
VL  - 55
IS  - 5
DO  - 10.3390/medicina55050155
ER  - 

@article{
author = "Đukanović, Ljubica and Lezaić, Višnja and Bukvić, Danica and Mirković, Duško and Marić, Ivko",
year = "2019",
abstract = "Background: A previous study indicated that Balkan endemic nephropathy (BEN) patients in the early stage of the disease had significantly higher creatinine clearance (Ccr) than healthy persons. The aim of the study was to assess whether tubular creatinine secretion affects Ccr in early stages of BEN and to check the applicability of serum creatinine-based glomerular filtration rate (GFR) equations in these patients. Methods: The study involved 21 BEN patients with estimated GFR (eGFR) above 60 mL/min/1.73 m(2), excluding any conditions that could affect GFR or tubular creatinine secretion, and 15 healthy controls. In all participants Ccr with and without cimetidine and iohexol clearance (mGFR) were measured and eGFR calculated using Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) and Modification of Diet in Renal Disease Study (MDRD) equations. Glomerular hyperfiltration cutoff (GFR-HF) was calculated. Results: There was no significant difference between the groups in Ccr before and after cimetidine or for eGFR, but mGFR was significantly higher in BEN patients than in controls (122.02 +/- 28.03 mL/min/1.73 m(2) vs. 101.15 +/- 27.32 mL/min/1.73 m(2); p = 0.032). Cimetidine administration reduced Ccr by 10% in both groups. The ratio of Ccr to mGFR was significantly above one in seven BEN patients and five controls and their mGFR values were similar. Seven other patients and eight controls had this ratio equal to one, while values below one were recorded for seven more patients and two controls. mGFR of all these 14 patients was significantly higher than that of healthy controls (129.88 +/- 27.52 mL/min/1.73 m(2) vs. 107.43 +/- 19.51 mL/min/1.73 m(2); p = 0.009). Mean GFR-HF was significantly higher than mGFR in controls, but these two values were similar in BEN patients. eGFR underestimated mGFR in both BEN patients and controls. Conclusion: The ratio of Ccr to mGFR and mGFR to GFR-HF indicated that elevated mGFR in early stages of BEN could be explained by increased glomerular filtration, but tubular creatinine secretion augmented Ccr in a smaller proportion of patients, who did not differ from healthy subjects.",
publisher = "MDPI, Basel",
journal = "Medicina-Lithuania",
title = "Increased Glomerular Filtration Rate in Early Stage of Balkan Endemic Nephropathy",
volume = "55",
number = "5",
doi = "10.3390/medicina55050155"
}

Đukanović, L., Lezaić, V., Bukvić, D., Mirković, D.,& Marić, I.. (2019). Increased Glomerular Filtration Rate in Early Stage of Balkan Endemic Nephropathy. in Medicina-Lithuania
MDPI, Basel., 55(5).
https://doi.org/10.3390/medicina55050155

Đukanović L, Lezaić V, Bukvić D, Mirković D, Marić I. Increased Glomerular Filtration Rate in Early Stage of Balkan Endemic Nephropathy. in Medicina-Lithuania. 2019;55(5).
doi:10.3390/medicina55050155 .

Đukanović, Ljubica, Lezaić, Višnja, Bukvić, Danica, Mirković, Duško, Marić, Ivko, "Increased Glomerular Filtration Rate in Early Stage of Balkan Endemic Nephropathy" in Medicina-Lithuania, 55, no. 5 (2019),
https://doi.org/10.3390/medicina55050155 . .

TY  - JOUR
AU  - Petrović, Jelena
AU  - Stanić, Dušanka
AU  - Bulat, Zorica
AU  - Puskas, Nela
AU  - Labudović-Borović, Milica
AU  - Batinić, Bojan
AU  - Mirković, Duško
AU  - Ignjatović, Svetlana
AU  - Pešić, Vesna
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3042
AB  - Magnesium (Mg), is not only a modulator of the glutamatergic NMDA receptors' affinity, it also prevents HPA axis hyperactivity, thus possibly being implicated in neurobiological features of mood disorders. Further uncovering of molecular mechanisms underlying magnesium's proposed effects is needed due to the recent shift in research of treatment resistant depression (TRD) towards glutamatergic pathways. Here, we applied Mg via drinking water for 28 days (50 mg/kg/day), in ACTH-treated rats, an established animal model of depression resistant to tricyclic antidepressants. Using this model in male rats we measured (1) changes in hippocampal neurogenesis and behavioral alterations, (2) adrenal hormones response to acute stress challenge and (3) levels of biometals involved in regulation of monoamines turnover in rat prefrontal cortex. Our results support beneficial behavioral impact of Mg in TRD model together with increased hippocampal neurogenesis and BDNF expression. Furthermore, Mg prevented ACTH-induced disruption in HPA axis function, by normalizing the levels of plasma ACTH, corticosterone and interleukin-6, and by increasing the peripheral release of adrenaline, noradrenaline and serotonin after the acute stress challenge. Finally, the influence on copper/zinc ratio suggested probable magnesium's involvement in monoamine turnover in PFC. Our findings provide further insights into the possible pathways implicated in the behavioral modulation effects of Mg, as well as its central and peripheral effects in ACTH-induced TRD model. Thus, further investigation of molecular signaling related to the glutamatergic transmission and role of Mg, could reveal prospects to novel treatment strategies that could be of particular importance for patients suffering from TRD.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Hormones and Behavior
T1  - Acth-induced model of depression resistant to tricyclic antidepressants: Neuroendocrine and behavioral changes and influence of long-term magnesium administration
VL  - 105
SP  - 1
EP  - 10
DO  - 10.1016/j.yhbeh.2018.07.003
ER  - 

@article{
author = "Petrović, Jelena and Stanić, Dušanka and Bulat, Zorica and Puskas, Nela and Labudović-Borović, Milica and Batinić, Bojan and Mirković, Duško and Ignjatović, Svetlana and Pešić, Vesna",
year = "2018",
abstract = "Magnesium (Mg), is not only a modulator of the glutamatergic NMDA receptors' affinity, it also prevents HPA axis hyperactivity, thus possibly being implicated in neurobiological features of mood disorders. Further uncovering of molecular mechanisms underlying magnesium's proposed effects is needed due to the recent shift in research of treatment resistant depression (TRD) towards glutamatergic pathways. Here, we applied Mg via drinking water for 28 days (50 mg/kg/day), in ACTH-treated rats, an established animal model of depression resistant to tricyclic antidepressants. Using this model in male rats we measured (1) changes in hippocampal neurogenesis and behavioral alterations, (2) adrenal hormones response to acute stress challenge and (3) levels of biometals involved in regulation of monoamines turnover in rat prefrontal cortex. Our results support beneficial behavioral impact of Mg in TRD model together with increased hippocampal neurogenesis and BDNF expression. Furthermore, Mg prevented ACTH-induced disruption in HPA axis function, by normalizing the levels of plasma ACTH, corticosterone and interleukin-6, and by increasing the peripheral release of adrenaline, noradrenaline and serotonin after the acute stress challenge. Finally, the influence on copper/zinc ratio suggested probable magnesium's involvement in monoamine turnover in PFC. Our findings provide further insights into the possible pathways implicated in the behavioral modulation effects of Mg, as well as its central and peripheral effects in ACTH-induced TRD model. Thus, further investigation of molecular signaling related to the glutamatergic transmission and role of Mg, could reveal prospects to novel treatment strategies that could be of particular importance for patients suffering from TRD.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Hormones and Behavior",
title = "Acth-induced model of depression resistant to tricyclic antidepressants: Neuroendocrine and behavioral changes and influence of long-term magnesium administration",
volume = "105",
pages = "1-10",
doi = "10.1016/j.yhbeh.2018.07.003"
}

Petrović, J., Stanić, D., Bulat, Z., Puskas, N., Labudović-Borović, M., Batinić, B., Mirković, D., Ignjatović, S.,& Pešić, V.. (2018). Acth-induced model of depression resistant to tricyclic antidepressants: Neuroendocrine and behavioral changes and influence of long-term magnesium administration. in Hormones and Behavior
Academic Press Inc Elsevier Science, San Diego., 105, 1-10.
https://doi.org/10.1016/j.yhbeh.2018.07.003

Petrović J, Stanić D, Bulat Z, Puskas N, Labudović-Borović M, Batinić B, Mirković D, Ignjatović S, Pešić V. Acth-induced model of depression resistant to tricyclic antidepressants: Neuroendocrine and behavioral changes and influence of long-term magnesium administration. in Hormones and Behavior. 2018;105:1-10.
doi:10.1016/j.yhbeh.2018.07.003 .

Petrović, Jelena, Stanić, Dušanka, Bulat, Zorica, Puskas, Nela, Labudović-Borović, Milica, Batinić, Bojan, Mirković, Duško, Ignjatović, Svetlana, Pešić, Vesna, "Acth-induced model of depression resistant to tricyclic antidepressants: Neuroendocrine and behavioral changes and influence of long-term magnesium administration" in Hormones and Behavior, 105 (2018):1-10,
https://doi.org/10.1016/j.yhbeh.2018.07.003 . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Sarić, Marija
AU  - Stojanović, Ljudmila
AU  - Mirković, Duško
AU  - Dopsaj, Violeta
AU  - Ignjatović, Svetlana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3063
AB  - Vascular antiphospholipid syndrome (VAPS) and obstetric (OAPS) are different entities because some patients only develop thrombosis (without recurrent pregnancy losses) and vice versa. Only two articles have reported that low 25-hydroxy-cholecalciferol (vitamin D3, VD3) levels were not correlated with the presence of conventional antiphospholipid antibodies (aPL Abs: anticardiolipin (aCL), anti-beta2glycoprotein I (a2gpI), and lupus anticoagulant (LA)), but no article analyzed the association of VD3 and anti-annexin A5 (aanxA5) Abs. The aim of our study was to investigate the association between VD3, multiple positivity of conventional aPL and aanxA5 Abs levels only in female OAPS vs. VAPS. Our study included 62 consecutive female PAPS patients. Concentrations of Abs were measured by ELISA, while VD3 levels were determined by immunochemiluminescence. Only 10/62 (16.13%) had sufficient (30ng/ml) VD3 levels, while 48/62 (77.42%) and 4/62 (6.45%) had insufficiency and VD3 deficiency, respectively. Statistically significant VD3 deficiency was noticed in VAPS (vs. OAPS, P=0.013). A negative correlation between VD3 levels and the age of patients was noticed (r=-0.493, P=0.032) only in VAPS subgroup. Multiple positivity of aPL and aanxA5 Abs was not associated with VD3 deficiency. Newly emerging aPL Abs, such as aanxA5 Abs, or their combinations with classical aPL Abs are not associated with VD3 deficiency in neither OAPS nor VAPS patients. Due to its immunomodulatory roles in B-Ly homeostasis, supplementation with VD3 should be considered in APS, at least in subgroup with severe form of the disease, i.e., VAPS.
PB  - Springer London Ltd, London
T2  - Clinical Rheumatology
T1  - Anti-annexin A5 antibodies and 25-hydroxy-cholecalciferol in female patients with primary antiphospholipid syndrome
VL  - 37
IS  - 12
SP  - 3359
EP  - 3364
DO  - 10.1007/s10067-018-4170-7
ER  - 

@article{
author = "Becarević, Mirjana and Sarić, Marija and Stojanović, Ljudmila and Mirković, Duško and Dopsaj, Violeta and Ignjatović, Svetlana",
year = "2018",
abstract = "Vascular antiphospholipid syndrome (VAPS) and obstetric (OAPS) are different entities because some patients only develop thrombosis (without recurrent pregnancy losses) and vice versa. Only two articles have reported that low 25-hydroxy-cholecalciferol (vitamin D3, VD3) levels were not correlated with the presence of conventional antiphospholipid antibodies (aPL Abs: anticardiolipin (aCL), anti-beta2glycoprotein I (a2gpI), and lupus anticoagulant (LA)), but no article analyzed the association of VD3 and anti-annexin A5 (aanxA5) Abs. The aim of our study was to investigate the association between VD3, multiple positivity of conventional aPL and aanxA5 Abs levels only in female OAPS vs. VAPS. Our study included 62 consecutive female PAPS patients. Concentrations of Abs were measured by ELISA, while VD3 levels were determined by immunochemiluminescence. Only 10/62 (16.13%) had sufficient (30ng/ml) VD3 levels, while 48/62 (77.42%) and 4/62 (6.45%) had insufficiency and VD3 deficiency, respectively. Statistically significant VD3 deficiency was noticed in VAPS (vs. OAPS, P=0.013). A negative correlation between VD3 levels and the age of patients was noticed (r=-0.493, P=0.032) only in VAPS subgroup. Multiple positivity of aPL and aanxA5 Abs was not associated with VD3 deficiency. Newly emerging aPL Abs, such as aanxA5 Abs, or their combinations with classical aPL Abs are not associated with VD3 deficiency in neither OAPS nor VAPS patients. Due to its immunomodulatory roles in B-Ly homeostasis, supplementation with VD3 should be considered in APS, at least in subgroup with severe form of the disease, i.e., VAPS.",
publisher = "Springer London Ltd, London",
journal = "Clinical Rheumatology",
title = "Anti-annexin A5 antibodies and 25-hydroxy-cholecalciferol in female patients with primary antiphospholipid syndrome",
volume = "37",
number = "12",
pages = "3359-3364",
doi = "10.1007/s10067-018-4170-7"
}

Becarević, M., Sarić, M., Stojanović, L., Mirković, D., Dopsaj, V.,& Ignjatović, S.. (2018). Anti-annexin A5 antibodies and 25-hydroxy-cholecalciferol in female patients with primary antiphospholipid syndrome. in Clinical Rheumatology
Springer London Ltd, London., 37(12), 3359-3364.
https://doi.org/10.1007/s10067-018-4170-7

Becarević M, Sarić M, Stojanović L, Mirković D, Dopsaj V, Ignjatović S. Anti-annexin A5 antibodies and 25-hydroxy-cholecalciferol in female patients with primary antiphospholipid syndrome. in Clinical Rheumatology. 2018;37(12):3359-3364.
doi:10.1007/s10067-018-4170-7 .

Becarević, Mirjana, Sarić, Marija, Stojanović, Ljudmila, Mirković, Duško, Dopsaj, Violeta, Ignjatović, Svetlana, "Anti-annexin A5 antibodies and 25-hydroxy-cholecalciferol in female patients with primary antiphospholipid syndrome" in Clinical Rheumatology, 37, no. 12 (2018):3359-3364,
https://doi.org/10.1007/s10067-018-4170-7 . .

TY  - JOUR
AU  - Dejanović, Bratislav
AU  - Vuković-Dejanović, Vesna
AU  - Ninković, Milica
AU  - Lavrnja, Irena
AU  - Stojanović, Ivana
AU  - Pavlović, Miloš
AU  - Begović, Vesna
AU  - Mirković, Duško
AU  - Stevanović, Ivana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3222
AB  - This study was aimed to study the potentially beneficial effects of agmatine on oxidative/nitrosative stress development in the brain of Wistar rats during subacute chlorpromazine treatment. The animals were divided into control (0.9% saline), chlorpromazine (38.7 mg/kg b.w.), chlorpromazine+agmatine (agmatine 75 mg/kg b.w. immediately after chlorpromazine, 38.7 mg/ kg b.w. i.p.) and agmatine (75 mg/kg b.w.) groups. All the tested substances were administered intraperitoneally for 15 consecutive days and the rats were sacrificed by decapitation on day 15. Subacute administration of chlorpromazine resulted in increased lipid peroxidation, nitric oxide concentration and superoxide anion production, while completely damaging the antioxidant defence system in the cerebral cortex, striatum, and hippocampus. However, the combined treatment with chlorpromazine and agmatine significantly attenuated the oxidative/nitrosative stress indices and restored the antioxidant capacity to the control values in all of the examined brain regions. Western blot analysis supported biochemical findings in all groups, but the most notable changes were found in the hippocampus. Our results suggest potentially beneficial effects of agmatine, which may be useful in the modified antioxidant approach in chlorpromazine-therapy.
PB  - Veterinarni A Farmaceuticka Univerzita Brno, Brno
T2  - Acta Veterinaria Brno
T1  - Effects of agmatine on chlorpromazine-induced neuronal injury in rat
VL  - 87
IS  - 2
SP  - 145
EP  - 153
DO  - 10.2754/avb201887020145
ER  - 

@article{
author = "Dejanović, Bratislav and Vuković-Dejanović, Vesna and Ninković, Milica and Lavrnja, Irena and Stojanović, Ivana and Pavlović, Miloš and Begović, Vesna and Mirković, Duško and Stevanović, Ivana",
year = "2018",
abstract = "This study was aimed to study the potentially beneficial effects of agmatine on oxidative/nitrosative stress development in the brain of Wistar rats during subacute chlorpromazine treatment. The animals were divided into control (0.9% saline), chlorpromazine (38.7 mg/kg b.w.), chlorpromazine+agmatine (agmatine 75 mg/kg b.w. immediately after chlorpromazine, 38.7 mg/ kg b.w. i.p.) and agmatine (75 mg/kg b.w.) groups. All the tested substances were administered intraperitoneally for 15 consecutive days and the rats were sacrificed by decapitation on day 15. Subacute administration of chlorpromazine resulted in increased lipid peroxidation, nitric oxide concentration and superoxide anion production, while completely damaging the antioxidant defence system in the cerebral cortex, striatum, and hippocampus. However, the combined treatment with chlorpromazine and agmatine significantly attenuated the oxidative/nitrosative stress indices and restored the antioxidant capacity to the control values in all of the examined brain regions. Western blot analysis supported biochemical findings in all groups, but the most notable changes were found in the hippocampus. Our results suggest potentially beneficial effects of agmatine, which may be useful in the modified antioxidant approach in chlorpromazine-therapy.",
publisher = "Veterinarni A Farmaceuticka Univerzita Brno, Brno",
journal = "Acta Veterinaria Brno",
title = "Effects of agmatine on chlorpromazine-induced neuronal injury in rat",
volume = "87",
number = "2",
pages = "145-153",
doi = "10.2754/avb201887020145"
}

Dejanović, B., Vuković-Dejanović, V., Ninković, M., Lavrnja, I., Stojanović, I., Pavlović, M., Begović, V., Mirković, D.,& Stevanović, I.. (2018). Effects of agmatine on chlorpromazine-induced neuronal injury in rat. in Acta Veterinaria Brno
Veterinarni A Farmaceuticka Univerzita Brno, Brno., 87(2), 145-153.
https://doi.org/10.2754/avb201887020145

Dejanović B, Vuković-Dejanović V, Ninković M, Lavrnja I, Stojanović I, Pavlović M, Begović V, Mirković D, Stevanović I. Effects of agmatine on chlorpromazine-induced neuronal injury in rat. in Acta Veterinaria Brno. 2018;87(2):145-153.
doi:10.2754/avb201887020145 .

Dejanović, Bratislav, Vuković-Dejanović, Vesna, Ninković, Milica, Lavrnja, Irena, Stojanović, Ivana, Pavlović, Miloš, Begović, Vesna, Mirković, Duško, Stevanović, Ivana, "Effects of agmatine on chlorpromazine-induced neuronal injury in rat" in Acta Veterinaria Brno, 87, no. 2 (2018):145-153,
https://doi.org/10.2754/avb201887020145 . .

TY  - CONF
AU  - Petrović, Jelena
AU  - Stanić, Dušanka
AU  - Puškaš, Nela
AU  - Oved, K.
AU  - Gurwitz, D.
AU  - Mirković, Duško
AU  - Plećaš, Bosiljka
AU  - Pešić, Vesna
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2870
PB  - Elsevier Science BV, Amsterdam
C3  - European Neuropsychopharmacology
T1  - Oxytocin promotes neurotrophic growth, increases integrin subunit beta 3 (ITGB3) and ameliorates depressive- and anxiety-like behaviour in rats
VL  - 27
IS  - Supplement 1
SP  - S34
EP  - S35
DO  - 10.1016/S0924-977X(17)30104-9
ER  - 

@conference{
author = "Petrović, Jelena and Stanić, Dušanka and Puškaš, Nela and Oved, K. and Gurwitz, D. and Mirković, Duško and Plećaš, Bosiljka and Pešić, Vesna",
year = "2017",
publisher = "Elsevier Science BV, Amsterdam",
journal = "European Neuropsychopharmacology",
title = "Oxytocin promotes neurotrophic growth, increases integrin subunit beta 3 (ITGB3) and ameliorates depressive- and anxiety-like behaviour in rats",
volume = "27",
number = "Supplement 1",
pages = "S34-S35",
doi = "10.1016/S0924-977X(17)30104-9"
}

Petrović, J., Stanić, D., Puškaš, N., Oved, K., Gurwitz, D., Mirković, D., Plećaš, B.,& Pešić, V.. (2017). Oxytocin promotes neurotrophic growth, increases integrin subunit beta 3 (ITGB3) and ameliorates depressive- and anxiety-like behaviour in rats. in European Neuropsychopharmacology
Elsevier Science BV, Amsterdam., 27(Supplement 1), S34-S35.
https://doi.org/10.1016/S0924-977X(17)30104-9

Petrović J, Stanić D, Puškaš N, Oved K, Gurwitz D, Mirković D, Plećaš B, Pešić V. Oxytocin promotes neurotrophic growth, increases integrin subunit beta 3 (ITGB3) and ameliorates depressive- and anxiety-like behaviour in rats. in European Neuropsychopharmacology. 2017;27(Supplement 1):S34-S35.
doi:10.1016/S0924-977X(17)30104-9 .

Petrović, Jelena, Stanić, Dušanka, Puškaš, Nela, Oved, K., Gurwitz, D., Mirković, Duško, Plećaš, Bosiljka, Pešić, Vesna, "Oxytocin promotes neurotrophic growth, increases integrin subunit beta 3 (ITGB3) and ameliorates depressive- and anxiety-like behaviour in rats" in European Neuropsychopharmacology, 27, no. Supplement 1 (2017):S34-S35,
https://doi.org/10.1016/S0924-977X(17)30104-9 . .

TY  - JOUR
AU  - Stanić, Dušanka
AU  - Plećaš-Solarović, Bosiljka
AU  - Mirković, Duško
AU  - Jovanović, Predrag
AU  - Dronjak, Slađana
AU  - Marković, Bojan
AU  - Dondević, Tea
AU  - Ignjatović, Svetlana
AU  - Pešić, Vesna
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3012
AB  - Chronic stress conditions can lead to considerable and extensible changes in physiological and psychological performances, and in emergence of risk for various somatic diseases. On the other hand, the neuropeptide oxytocin is reported to increase the resistance of the organism to stress and modulate activity of autonomic nervous system. Chronic corticosterone administration is used as a rat model for a state observed in terms of chronic stress exposure, when negative feedback mechanism of hypothalamus-pituitary-adrenal axis activity is disrupted. In our study, we aimed to investigate whether chronic administration of oxytocin (10114400 pI/day for 14 days, s.c.) influenced adrenal gland morphology and activity in adult male Wistar rats during long-term corticosterone administration via drinking water (100 mg/L for 21 days). We examined the influence of treatments on the levels of adrenal gland hormones, corticosterone, adrenaline and noradrenaline, as well as their response to an acute stress challenge evoked by 15-min forced swimming. In addition, the expression of two main monoamine transporters, the noradrenaline transporter (NAT) and vesicular monoamine transporter 2 (VMAT2) in adrenal medulla was measured in the rats exposed to acute stress. Our results showed that oxytocin treatment prevented corticosterone-induced decrease in body weight gain, attenuated adrenal gland atrophy by increasing glandular weight, and the area of the zona fasciculate and reticularis. Chronic corticosterone intake blunted the response of all measured hormones to acute stress, whereas concomitant oxytocin treatment reversed adrenaline and noradrenaline response to acute stress. Furthermore, in adrenal medulla, oxytocin produced significant vasodilatation and stimulated expression of both catecholamine transporters detected both on mRNA and protein level. Our data suggest that oxytocin, by reducing atrophy of adrenal gland, and by increasing catecholamine storage capacity, may be beneficial in conditions accompanied with high glucocorticoid levels, such as chronic stress exposure.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Psychopharmacology
T1  - Oxytocin in corticosterone-induced chronic stress model: Focus on adrenal gland function
VL  - 80
SP  - 137
EP  - 146
DO  - 10.1016/j.psyneuen.2017.03.011
ER  - 

@article{
author = "Stanić, Dušanka and Plećaš-Solarović, Bosiljka and Mirković, Duško and Jovanović, Predrag and Dronjak, Slađana and Marković, Bojan and Dondević, Tea and Ignjatović, Svetlana and Pešić, Vesna",
year = "2017",
abstract = "Chronic stress conditions can lead to considerable and extensible changes in physiological and psychological performances, and in emergence of risk for various somatic diseases. On the other hand, the neuropeptide oxytocin is reported to increase the resistance of the organism to stress and modulate activity of autonomic nervous system. Chronic corticosterone administration is used as a rat model for a state observed in terms of chronic stress exposure, when negative feedback mechanism of hypothalamus-pituitary-adrenal axis activity is disrupted. In our study, we aimed to investigate whether chronic administration of oxytocin (10114400 pI/day for 14 days, s.c.) influenced adrenal gland morphology and activity in adult male Wistar rats during long-term corticosterone administration via drinking water (100 mg/L for 21 days). We examined the influence of treatments on the levels of adrenal gland hormones, corticosterone, adrenaline and noradrenaline, as well as their response to an acute stress challenge evoked by 15-min forced swimming. In addition, the expression of two main monoamine transporters, the noradrenaline transporter (NAT) and vesicular monoamine transporter 2 (VMAT2) in adrenal medulla was measured in the rats exposed to acute stress. Our results showed that oxytocin treatment prevented corticosterone-induced decrease in body weight gain, attenuated adrenal gland atrophy by increasing glandular weight, and the area of the zona fasciculate and reticularis. Chronic corticosterone intake blunted the response of all measured hormones to acute stress, whereas concomitant oxytocin treatment reversed adrenaline and noradrenaline response to acute stress. Furthermore, in adrenal medulla, oxytocin produced significant vasodilatation and stimulated expression of both catecholamine transporters detected both on mRNA and protein level. Our data suggest that oxytocin, by reducing atrophy of adrenal gland, and by increasing catecholamine storage capacity, may be beneficial in conditions accompanied with high glucocorticoid levels, such as chronic stress exposure.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Psychopharmacology",
title = "Oxytocin in corticosterone-induced chronic stress model: Focus on adrenal gland function",
volume = "80",
pages = "137-146",
doi = "10.1016/j.psyneuen.2017.03.011"
}

Stanić, D., Plećaš-Solarović, B., Mirković, D., Jovanović, P., Dronjak, S., Marković, B., Dondević, T., Ignjatović, S.,& Pešić, V.. (2017). Oxytocin in corticosterone-induced chronic stress model: Focus on adrenal gland function. in Psychopharmacology
Pergamon-Elsevier Science Ltd, Oxford., 80, 137-146.
https://doi.org/10.1016/j.psyneuen.2017.03.011

Stanić D, Plećaš-Solarović B, Mirković D, Jovanović P, Dronjak S, Marković B, Dondević T, Ignjatović S, Pešić V. Oxytocin in corticosterone-induced chronic stress model: Focus on adrenal gland function. in Psychopharmacology. 2017;80:137-146.
doi:10.1016/j.psyneuen.2017.03.011 .

Stanić, Dušanka, Plećaš-Solarović, Bosiljka, Mirković, Duško, Jovanović, Predrag, Dronjak, Slađana, Marković, Bojan, Dondević, Tea, Ignjatović, Svetlana, Pešić, Vesna, "Oxytocin in corticosterone-induced chronic stress model: Focus on adrenal gland function" in Psychopharmacology, 80 (2017):137-146,
https://doi.org/10.1016/j.psyneuen.2017.03.011 . .

TY  - CONF
AU  - Petrović, Jelena
AU  - Stanić, Dušanka
AU  - Mirković, Duško
AU  - Batinić, Bojan
AU  - Plećaš, Bosiljka
AU  - Ignjatović, Svetlana
AU  - Pešić, Vesna
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2687
PB  - Elsevier Science BV, Amsterdam
C3  - European Neuropsychopharmacology
T1  - Effects of long-term magnesium administration on levels of stress hormones and interleukin-6 after acute stress in rats chronically treated with adrenocorticotropic hormone
VL  - 26
IS  - Supplement 2
SP  - S207
EP  - S207
DO  - 10.1016/S0924-977X(16)31053-7
ER  - 

@conference{
author = "Petrović, Jelena and Stanić, Dušanka and Mirković, Duško and Batinić, Bojan and Plećaš, Bosiljka and Ignjatović, Svetlana and Pešić, Vesna",
year = "2016",
publisher = "Elsevier Science BV, Amsterdam",
journal = "European Neuropsychopharmacology",
title = "Effects of long-term magnesium administration on levels of stress hormones and interleukin-6 after acute stress in rats chronically treated with adrenocorticotropic hormone",
volume = "26",
number = "Supplement 2",
pages = "S207-S207",
doi = "10.1016/S0924-977X(16)31053-7"
}

Petrović, J., Stanić, D., Mirković, D., Batinić, B., Plećaš, B., Ignjatović, S.,& Pešić, V.. (2016). Effects of long-term magnesium administration on levels of stress hormones and interleukin-6 after acute stress in rats chronically treated with adrenocorticotropic hormone. in European Neuropsychopharmacology
Elsevier Science BV, Amsterdam., 26(Supplement 2), S207-S207.
https://doi.org/10.1016/S0924-977X(16)31053-7

Petrović J, Stanić D, Mirković D, Batinić B, Plećaš B, Ignjatović S, Pešić V. Effects of long-term magnesium administration on levels of stress hormones and interleukin-6 after acute stress in rats chronically treated with adrenocorticotropic hormone. in European Neuropsychopharmacology. 2016;26(Supplement 2):S207-S207.
doi:10.1016/S0924-977X(16)31053-7 .

Petrović, Jelena, Stanić, Dušanka, Mirković, Duško, Batinić, Bojan, Plećaš, Bosiljka, Ignjatović, Svetlana, Pešić, Vesna, "Effects of long-term magnesium administration on levels of stress hormones and interleukin-6 after acute stress in rats chronically treated with adrenocorticotropic hormone" in European Neuropsychopharmacology, 26, no. Supplement 2 (2016):S207-S207,
https://doi.org/10.1016/S0924-977X(16)31053-7 . .

TY  - CONF
AU  - Stanić, Dušanka
AU  - Petrović, Jelena
AU  - Mirković, Duško
AU  - Đorđević, Tea
AU  - Durić, V.
AU  - Jovanović, P.
AU  - Dronjak, Slađana
AU  - Pešić, Vesna
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2686
PB  - Elsevier Science BV, Amsterdam
C3  - European Neuropsychopharmacology
T1  - Oxytocin modulates HPA axis activity and hormone response to stress in rats chronically treated with corticosterone
VL  - 26
IS  - Supplement 2
SP  - S204
EP  - S204
DO  - 10.1016/S0924-977X(16)31048-3
ER  - 

@conference{
author = "Stanić, Dušanka and Petrović, Jelena and Mirković, Duško and Đorđević, Tea and Durić, V. and Jovanović, P. and Dronjak, Slađana and Pešić, Vesna",
year = "2016",
publisher = "Elsevier Science BV, Amsterdam",
journal = "European Neuropsychopharmacology",
title = "Oxytocin modulates HPA axis activity and hormone response to stress in rats chronically treated with corticosterone",
volume = "26",
number = "Supplement 2",
pages = "S204-S204",
doi = "10.1016/S0924-977X(16)31048-3"
}

Stanić, D., Petrović, J., Mirković, D., Đorđević, T., Durić, V., Jovanović, P., Dronjak, S.,& Pešić, V.. (2016). Oxytocin modulates HPA axis activity and hormone response to stress in rats chronically treated with corticosterone. in European Neuropsychopharmacology
Elsevier Science BV, Amsterdam., 26(Supplement 2), S204-S204.
https://doi.org/10.1016/S0924-977X(16)31048-3

Stanić D, Petrović J, Mirković D, Đorđević T, Durić V, Jovanović P, Dronjak S, Pešić V. Oxytocin modulates HPA axis activity and hormone response to stress in rats chronically treated with corticosterone. in European Neuropsychopharmacology. 2016;26(Supplement 2):S204-S204.
doi:10.1016/S0924-977X(16)31048-3 .

Stanić, Dušanka, Petrović, Jelena, Mirković, Duško, Đorđević, Tea, Durić, V., Jovanović, P., Dronjak, Slađana, Pešić, Vesna, "Oxytocin modulates HPA axis activity and hormone response to stress in rats chronically treated with corticosterone" in European Neuropsychopharmacology, 26, no. Supplement 2 (2016):S204-S204,
https://doi.org/10.1016/S0924-977X(16)31048-3 . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Mirković, Duško
AU  - Ignjatović, Svetlana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2629
AB  - Although numerous studies investigated the association between homocysteine (Hcy), tumor necrosis factor alpha (TNF-alpha), C-reactive protein (CRP) and apolipoproteins (apo(s)) with thrombosis and/or recurrent pregnancy losses, studies that analyzed the abovementioned parameters and multiple positivity of antiphospholipid antibodies (aPL Abs) in patients with primary antiphospholipid syndrome (PAPS) are lacking. Therefore, the aim of this study was to analyze the presence of various combinations of the abovementioned parameters and their associations with clinical and/or serological features of PAPS. High-pressure liquid chromatography (HPLC) was used for determination of Hcy, while apoAI, apoB, and lipoprotein (Lp) (a) concentrations were estimated by immunonephelometry. High-sensitivity C-reactive protein (hsCRP) was measured by immunoturbidimetry. Apo (a), TNF-alpha, and aPL Abs were measured by ELISA. Various combinations of analyzed parameters (Hcy/CRP/TNF/apoAI/apoB/apo (a)/Lp (a)) were not associated with a single presence of either aPL Abs. Double positivity for both isotypes of anticardiolipin (aCL) Abs (IgG + IgM) was associated with the increased apoB levels. The presence of the IgG isotype of both aCL + anti-beta2 glycoprotein I (a beta 2gpI) Abs was associated with the highest number of analyzed parameters (i.e., increased levels of hsCRP, Lp (a), and apo (a)). The presence of the IgG isotype of both aCL + a beta 2gpI Abs was associated with the highest number of vascular impairment parameters in patients with PAPS, and this combination confers the highest risk for the recurrence of thrombotic episodes. This is the first report that analyzed the association between various combinations of vascular impairment parameters with multiple aPL Ab positivity. Our results provide a rationale for further investigations of therapeutic approaches for PAPS patients.
PB  - Springer London Ltd, London
T2  - Clinical Rheumatology
T1  - Double positivity of the IgG isotype of both anticardiolipin and anti-beta 2gpI antibodies is associated with the highest number of vascular impairment parameters in patients with primary antiphospholipid syndrome: preliminary data
VL  - 35
IS  - 12
SP  - 2947
EP  - 2954
DO  - 10.1007/s10067-016-3438-z
ER  - 

@article{
author = "Becarević, Mirjana and Mirković, Duško and Ignjatović, Svetlana",
year = "2016",
abstract = "Although numerous studies investigated the association between homocysteine (Hcy), tumor necrosis factor alpha (TNF-alpha), C-reactive protein (CRP) and apolipoproteins (apo(s)) with thrombosis and/or recurrent pregnancy losses, studies that analyzed the abovementioned parameters and multiple positivity of antiphospholipid antibodies (aPL Abs) in patients with primary antiphospholipid syndrome (PAPS) are lacking. Therefore, the aim of this study was to analyze the presence of various combinations of the abovementioned parameters and their associations with clinical and/or serological features of PAPS. High-pressure liquid chromatography (HPLC) was used for determination of Hcy, while apoAI, apoB, and lipoprotein (Lp) (a) concentrations were estimated by immunonephelometry. High-sensitivity C-reactive protein (hsCRP) was measured by immunoturbidimetry. Apo (a), TNF-alpha, and aPL Abs were measured by ELISA. Various combinations of analyzed parameters (Hcy/CRP/TNF/apoAI/apoB/apo (a)/Lp (a)) were not associated with a single presence of either aPL Abs. Double positivity for both isotypes of anticardiolipin (aCL) Abs (IgG + IgM) was associated with the increased apoB levels. The presence of the IgG isotype of both aCL + anti-beta2 glycoprotein I (a beta 2gpI) Abs was associated with the highest number of analyzed parameters (i.e., increased levels of hsCRP, Lp (a), and apo (a)). The presence of the IgG isotype of both aCL + a beta 2gpI Abs was associated with the highest number of vascular impairment parameters in patients with PAPS, and this combination confers the highest risk for the recurrence of thrombotic episodes. This is the first report that analyzed the association between various combinations of vascular impairment parameters with multiple aPL Ab positivity. Our results provide a rationale for further investigations of therapeutic approaches for PAPS patients.",
publisher = "Springer London Ltd, London",
journal = "Clinical Rheumatology",
title = "Double positivity of the IgG isotype of both anticardiolipin and anti-beta 2gpI antibodies is associated with the highest number of vascular impairment parameters in patients with primary antiphospholipid syndrome: preliminary data",
volume = "35",
number = "12",
pages = "2947-2954",
doi = "10.1007/s10067-016-3438-z"
}

Becarević, M., Mirković, D.,& Ignjatović, S.. (2016). Double positivity of the IgG isotype of both anticardiolipin and anti-beta 2gpI antibodies is associated with the highest number of vascular impairment parameters in patients with primary antiphospholipid syndrome: preliminary data. in Clinical Rheumatology
Springer London Ltd, London., 35(12), 2947-2954.
https://doi.org/10.1007/s10067-016-3438-z

Becarević M, Mirković D, Ignjatović S. Double positivity of the IgG isotype of both anticardiolipin and anti-beta 2gpI antibodies is associated with the highest number of vascular impairment parameters in patients with primary antiphospholipid syndrome: preliminary data. in Clinical Rheumatology. 2016;35(12):2947-2954.
doi:10.1007/s10067-016-3438-z .

Becarević, Mirjana, Mirković, Duško, Ignjatović, Svetlana, "Double positivity of the IgG isotype of both anticardiolipin and anti-beta 2gpI antibodies is associated with the highest number of vascular impairment parameters in patients with primary antiphospholipid syndrome: preliminary data" in Clinical Rheumatology, 35, no. 12 (2016):2947-2954,
https://doi.org/10.1007/s10067-016-3438-z . .

TY  - JOUR
AU  - Beletić, Anđelo
AU  - Mirković, Duško
AU  - Dudvarski-Ilić, Aleksandra
AU  - Milenković, Branislava
AU  - Nagorni-Obradović, Ljudmila
AU  - Đorđević, Valentina
AU  - Ignjatović, Svetlana
AU  - Majkić-Singh, Nada
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2299
AB  - Background: An increased homocysteine (Hcy) concentration may represent a metabolic marker of folate and vitamin B-12 deficiency, both significant public health problems. For different reasons, patients with chronic obstructive pulmonary disease (COPD) are prone to these deficiencies. The study evaluates the reliability of Hcy concentration in predicting folate or vitamin B-12 deficiency in these patients. Methods: A group of 50 COPD patients (28 males/22 females, age ((X) over bar +/- SD=49.0 +/- 14.5) years was enrolled. A chemiluminescent microparticle immunoassay was applied for homocysteine, folate and vitamin B-12 concentration. Kolmogorov-Smirnov, Mann-Whitney U and chi(2) tests, Spearman's correlation and ROC analysis were included in the statistical analysis, with the level of significance set at 0.05. Results: Average (SD) concentrations of folate and vitamin B-12 were 4.13 (2.16) mu g/L and 463.6 (271.0) ng/L, whereas only vitamin B-12 correlated with the Hcy level (P=-0.310 (R=0.029)). Gender related differences were not significant and only a borderline significant correlation between age and folate was confirmed (R=0.279 (P=0.047)). The incidence of folate and vitamin B-12 deficiency differed significantly (P=0.000 and P lt 0.000 for folate and vitamin B12 respectively), depending on the cutoff used for classification (4.4, 6.6 and 8.0 mu g/L folate; 203 and 473 ng/L - vitamin B-12). ROC analyses failed to show any significance of hyperhomocysteinemia as a predictor of folate or vitamin B-12 deficiency. Conclusion: Reliability of the Hcy concentration as a biomarker of folate or vitamin B-12 depletion in COPD patients is not satisfactory, so their deficiency cannot be predicted by the occurrence of HHcy.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Questionable Reliability of Homocysteine As the Metabolic Marker for Folate and Vitamin B12 Deficiency in Patients with Chronic Obstructive Pulmonary Disease
VL  - 34
IS  - 4
SP  - 467
EP  - 472
DO  - 10.2478/jomb-2014-0046
ER  - 

@article{
author = "Beletić, Anđelo and Mirković, Duško and Dudvarski-Ilić, Aleksandra and Milenković, Branislava and Nagorni-Obradović, Ljudmila and Đorđević, Valentina and Ignjatović, Svetlana and Majkić-Singh, Nada",
year = "2015",
abstract = "Background: An increased homocysteine (Hcy) concentration may represent a metabolic marker of folate and vitamin B-12 deficiency, both significant public health problems. For different reasons, patients with chronic obstructive pulmonary disease (COPD) are prone to these deficiencies. The study evaluates the reliability of Hcy concentration in predicting folate or vitamin B-12 deficiency in these patients. Methods: A group of 50 COPD patients (28 males/22 females, age ((X) over bar +/- SD=49.0 +/- 14.5) years was enrolled. A chemiluminescent microparticle immunoassay was applied for homocysteine, folate and vitamin B-12 concentration. Kolmogorov-Smirnov, Mann-Whitney U and chi(2) tests, Spearman's correlation and ROC analysis were included in the statistical analysis, with the level of significance set at 0.05. Results: Average (SD) concentrations of folate and vitamin B-12 were 4.13 (2.16) mu g/L and 463.6 (271.0) ng/L, whereas only vitamin B-12 correlated with the Hcy level (P=-0.310 (R=0.029)). Gender related differences were not significant and only a borderline significant correlation between age and folate was confirmed (R=0.279 (P=0.047)). The incidence of folate and vitamin B-12 deficiency differed significantly (P=0.000 and P lt 0.000 for folate and vitamin B12 respectively), depending on the cutoff used for classification (4.4, 6.6 and 8.0 mu g/L folate; 203 and 473 ng/L - vitamin B-12). ROC analyses failed to show any significance of hyperhomocysteinemia as a predictor of folate or vitamin B-12 deficiency. Conclusion: Reliability of the Hcy concentration as a biomarker of folate or vitamin B-12 depletion in COPD patients is not satisfactory, so their deficiency cannot be predicted by the occurrence of HHcy.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Questionable Reliability of Homocysteine As the Metabolic Marker for Folate and Vitamin B12 Deficiency in Patients with Chronic Obstructive Pulmonary Disease",
volume = "34",
number = "4",
pages = "467-472",
doi = "10.2478/jomb-2014-0046"
}

Beletić, A., Mirković, D., Dudvarski-Ilić, A., Milenković, B., Nagorni-Obradović, L., Đorđević, V., Ignjatović, S.,& Majkić-Singh, N.. (2015). Questionable Reliability of Homocysteine As the Metabolic Marker for Folate and Vitamin B12 Deficiency in Patients with Chronic Obstructive Pulmonary Disease. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 34(4), 467-472.
https://doi.org/10.2478/jomb-2014-0046

Beletić A, Mirković D, Dudvarski-Ilić A, Milenković B, Nagorni-Obradović L, Đorđević V, Ignjatović S, Majkić-Singh N. Questionable Reliability of Homocysteine As the Metabolic Marker for Folate and Vitamin B12 Deficiency in Patients with Chronic Obstructive Pulmonary Disease. in Journal of Medical Biochemistry. 2015;34(4):467-472.
doi:10.2478/jomb-2014-0046 .

Beletić, Anđelo, Mirković, Duško, Dudvarski-Ilić, Aleksandra, Milenković, Branislava, Nagorni-Obradović, Ljudmila, Đorđević, Valentina, Ignjatović, Svetlana, Majkić-Singh, Nada, "Questionable Reliability of Homocysteine As the Metabolic Marker for Folate and Vitamin B12 Deficiency in Patients with Chronic Obstructive Pulmonary Disease" in Journal of Medical Biochemistry, 34, no. 4 (2015):467-472,
https://doi.org/10.2478/jomb-2014-0046 . .

TY  - JOUR
AU  - Beletić, Anđelo
AU  - Dudvarski-Ilić, Aleksandra
AU  - Milenković, Branislava
AU  - Nagorni-Obradović, Ljudmila
AU  - Ljujić, Mila
AU  - Đorđević, Valentina
AU  - Mirković, Duško
AU  - Radojković, Dragica
AU  - Majkić-Singh, Nada
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2204
AB  - Introduction: Alpha-1-antitrypsin deficiency (AATD), genetic risk factor for premature chronic obstructive pulmonary disease (COPD), often remains undetected. The aim of our study was to analyse the effectiveness of an integrative laboratory algorithm for AATD detection in patients diagnosed with COPD by the age of 45 years, in comparison with the screening approach based on AAT concentration measurement alone. Subjects and methods: 50 unrelated patients (28 males / 22 females, age 52 (24-75 years) diagnosed with COPD before the age of 45 years were enrolled. Immunonephelometric assay for alpha-1-antitrypsin (AAT) and PCR-reverse hybridization for Z and S allele were first-line, and isoelectric focusing and DNA sequencing (ABI Prism BigDye) were reflex tests. Results: AATD associated genotypes were detected in 7 patients (5 ZZ, 1 ZM(malton), 1 ZQ0(amersfoort)), 10 were heterozygous carriers (8 MZ and 2 MS genotypes) and 33 were without AATD (MM genotype). Carriers and patients without AATD had comparable AAT concentrations (P = 0.125). In majority of participants (48) first line tests were sufficient to analyze AATD presence. In two remaining cases reflex tests identified rare alleles, M-malton and Q0(amersfoort), the later one being reported for the first time in Serbian population. Detection rate did not differ between algorithm and screening both for AATD (P = 0.500) and carriers (P = 0.063). Conclusion: There is a high prevalence of AATD affected subjects and carriers in a group of patients with premature COPD. The use of integrative laboratory algorithm does not improve the effectiveness of AATD detection in comparison with the screening based on AAT concentration alone.
PB  - Croatian Soc Medical Biochemists, Zagreb
T2  - Biochemia Medica
T1  - Is an integrative laboratory algorithm more effective in detecting alpha-1-antitrypsin deficiency in patients with premature chronic obstructive pulmonary disease than AAT concentration based screening approach?
VL  - 24
IS  - 2
SP  - 293
EP  - 298
DO  - 10.11613/BM.2014.032
ER  - 

@article{
author = "Beletić, Anđelo and Dudvarski-Ilić, Aleksandra and Milenković, Branislava and Nagorni-Obradović, Ljudmila and Ljujić, Mila and Đorđević, Valentina and Mirković, Duško and Radojković, Dragica and Majkić-Singh, Nada",
year = "2014",
abstract = "Introduction: Alpha-1-antitrypsin deficiency (AATD), genetic risk factor for premature chronic obstructive pulmonary disease (COPD), often remains undetected. The aim of our study was to analyse the effectiveness of an integrative laboratory algorithm for AATD detection in patients diagnosed with COPD by the age of 45 years, in comparison with the screening approach based on AAT concentration measurement alone. Subjects and methods: 50 unrelated patients (28 males / 22 females, age 52 (24-75 years) diagnosed with COPD before the age of 45 years were enrolled. Immunonephelometric assay for alpha-1-antitrypsin (AAT) and PCR-reverse hybridization for Z and S allele were first-line, and isoelectric focusing and DNA sequencing (ABI Prism BigDye) were reflex tests. Results: AATD associated genotypes were detected in 7 patients (5 ZZ, 1 ZM(malton), 1 ZQ0(amersfoort)), 10 were heterozygous carriers (8 MZ and 2 MS genotypes) and 33 were without AATD (MM genotype). Carriers and patients without AATD had comparable AAT concentrations (P = 0.125). In majority of participants (48) first line tests were sufficient to analyze AATD presence. In two remaining cases reflex tests identified rare alleles, M-malton and Q0(amersfoort), the later one being reported for the first time in Serbian population. Detection rate did not differ between algorithm and screening both for AATD (P = 0.500) and carriers (P = 0.063). Conclusion: There is a high prevalence of AATD affected subjects and carriers in a group of patients with premature COPD. The use of integrative laboratory algorithm does not improve the effectiveness of AATD detection in comparison with the screening based on AAT concentration alone.",
publisher = "Croatian Soc Medical Biochemists, Zagreb",
journal = "Biochemia Medica",
title = "Is an integrative laboratory algorithm more effective in detecting alpha-1-antitrypsin deficiency in patients with premature chronic obstructive pulmonary disease than AAT concentration based screening approach?",
volume = "24",
number = "2",
pages = "293-298",
doi = "10.11613/BM.2014.032"
}

Beletić, A., Dudvarski-Ilić, A., Milenković, B., Nagorni-Obradović, L., Ljujić, M., Đorđević, V., Mirković, D., Radojković, D.,& Majkić-Singh, N.. (2014). Is an integrative laboratory algorithm more effective in detecting alpha-1-antitrypsin deficiency in patients with premature chronic obstructive pulmonary disease than AAT concentration based screening approach?. in Biochemia Medica
Croatian Soc Medical Biochemists, Zagreb., 24(2), 293-298.
https://doi.org/10.11613/BM.2014.032

Beletić A, Dudvarski-Ilić A, Milenković B, Nagorni-Obradović L, Ljujić M, Đorđević V, Mirković D, Radojković D, Majkić-Singh N. Is an integrative laboratory algorithm more effective in detecting alpha-1-antitrypsin deficiency in patients with premature chronic obstructive pulmonary disease than AAT concentration based screening approach?. in Biochemia Medica. 2014;24(2):293-298.
doi:10.11613/BM.2014.032 .

Beletić, Anđelo, Dudvarski-Ilić, Aleksandra, Milenković, Branislava, Nagorni-Obradović, Ljudmila, Ljujić, Mila, Đorđević, Valentina, Mirković, Duško, Radojković, Dragica, Majkić-Singh, Nada, "Is an integrative laboratory algorithm more effective in detecting alpha-1-antitrypsin deficiency in patients with premature chronic obstructive pulmonary disease than AAT concentration based screening approach?" in Biochemia Medica, 24, no. 2 (2014):293-298,
https://doi.org/10.11613/BM.2014.032 . .

TY  - JOUR
AU  - Novaković, Ivana
AU  - Maksimović, Nela
AU  - Pavlović, Aleksandra
AU  - Žarković, Milena
AU  - Rovcanin, Branislav
AU  - Mirković, Duško
AU  - Pekmezović, Tatjana
AU  - Cvetković, Dragana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2148
AB  - Molecular genetic testing is part of modern medical practice. DNA tests are an essential part of diagnostics and genetic counseling in single gene diseases, while their application in polygenic disorders is still limited. Pharmacogenetics studies DNA variants associated with variations in drug efficacy and toxicity, and tests in this field are being developed rapidly. The main method for molecular genetic testing is the polymerase chain reaction, with a number of modifications. New methods, such as next generation sequencing and DNA microarray, should allow simultaneous analysis of a number of genes, even whole genome sequencing. Ethical concerns in molecular genetic testing are very important, along with legislation. After molecular genetic testing, interpretation of results and genetic counseling should be done by professionals. With the example of thrombophilia, we discuss questions about genetic testing, its possibilities and promises.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Introduction to Molecular genetic diagnostics
VL  - 33
IS  - 1
SP  - 3
EP  - 7
DO  - 10.2478/jomb-2013-0039
ER  - 

@article{
author = "Novaković, Ivana and Maksimović, Nela and Pavlović, Aleksandra and Žarković, Milena and Rovcanin, Branislav and Mirković, Duško and Pekmezović, Tatjana and Cvetković, Dragana",
year = "2014",
abstract = "Molecular genetic testing is part of modern medical practice. DNA tests are an essential part of diagnostics and genetic counseling in single gene diseases, while their application in polygenic disorders is still limited. Pharmacogenetics studies DNA variants associated with variations in drug efficacy and toxicity, and tests in this field are being developed rapidly. The main method for molecular genetic testing is the polymerase chain reaction, with a number of modifications. New methods, such as next generation sequencing and DNA microarray, should allow simultaneous analysis of a number of genes, even whole genome sequencing. Ethical concerns in molecular genetic testing are very important, along with legislation. After molecular genetic testing, interpretation of results and genetic counseling should be done by professionals. With the example of thrombophilia, we discuss questions about genetic testing, its possibilities and promises.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Introduction to Molecular genetic diagnostics",
volume = "33",
number = "1",
pages = "3-7",
doi = "10.2478/jomb-2013-0039"
}

Novaković, I., Maksimović, N., Pavlović, A., Žarković, M., Rovcanin, B., Mirković, D., Pekmezović, T.,& Cvetković, D.. (2014). Introduction to Molecular genetic diagnostics. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 33(1), 3-7.
https://doi.org/10.2478/jomb-2013-0039

Novaković I, Maksimović N, Pavlović A, Žarković M, Rovcanin B, Mirković D, Pekmezović T, Cvetković D. Introduction to Molecular genetic diagnostics. in Journal of Medical Biochemistry. 2014;33(1):3-7.
doi:10.2478/jomb-2013-0039 .

Novaković, Ivana, Maksimović, Nela, Pavlović, Aleksandra, Žarković, Milena, Rovcanin, Branislav, Mirković, Duško, Pekmezović, Tatjana, Cvetković, Dragana, "Introduction to Molecular genetic diagnostics" in Journal of Medical Biochemistry, 33, no. 1 (2014):3-7,
https://doi.org/10.2478/jomb-2013-0039 . .

TY  - JOUR
AU  - Culafić-Vojinović, Violeta
AU  - Ćulafić, Đorđe
AU  - Ignjatović, Svetlana
AU  - Petakov, Milan
AU  - Đurović-Nikolić, Marina
AU  - Vasić, Jelena
AU  - Mirković, Duško
AU  - Mijac, Dragana
AU  - Stulić, Miloš
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2146
AB  - Background: Metabolic bone disease in patients with chronic liver disease is called hepatic osteodystrophy and is primarily a sequel to osteopenia/osteoporosis, and rarely secondary to osteomalacia: The aim of this work was to define the influence of vitamin D-3 and parathyroid hormone (PTH) in the pathogenesis of hepatic osteodystrophy, as well as the predictive significance of biochemical bone markers. Methods: This prospective study included 58 male patients with alcoholic (49) and viral (9) cirrhosis. The concentrations of serum vitamin D-3, PTH, osteocalcin and beta-carboxy-terminal cross-linked telopeptide of type I collagen (beta-CTX) were determined. Bone density was measured by dual energy X-ray absorptiometry in the L1 -L4 spinal segment and the femoral neck. Results: Lower bone mineral density (BMD) was measured in 41 patients (70.7%). There was no significant correlation between PTH and vitamin D3 values and T score in the femoral neck (p=0.51; p=0.063) and lumbar spine (p=0.49; 0.064). Also, no significant correlation was found between the osteocalcin values in lumbar spine BMD (p=0.944) and femoral neck (p=0.161), or with beta-CTX values and BMD in the lumbar spine (p=0.347) and femoral neck (p=0.73). Statistically significant difference was confirmed between the stage A osteocalcin (p=0.000) and beta-CTX (p=0.008) values in relation to advanced stages B and C. Conclusions: PTH and vitamin D3 do not influence the development of hepatic osteodystrophy. In patients with cirrhosis, osteocalcin and beta-CTX are not valid indicators of decreased BMD, but their values correlate with the degree of liver insufficiency.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - The clinical importance of biochemical bone markers in patients with alcoholic and viral liver cirrhosis
VL  - 33
IS  - 2
SP  - 149
EP  - 155
DO  - 10.2478/jomb-2013-0025
ER  - 

@article{
author = "Culafić-Vojinović, Violeta and Ćulafić, Đorđe and Ignjatović, Svetlana and Petakov, Milan and Đurović-Nikolić, Marina and Vasić, Jelena and Mirković, Duško and Mijac, Dragana and Stulić, Miloš",
year = "2014",
abstract = "Background: Metabolic bone disease in patients with chronic liver disease is called hepatic osteodystrophy and is primarily a sequel to osteopenia/osteoporosis, and rarely secondary to osteomalacia: The aim of this work was to define the influence of vitamin D-3 and parathyroid hormone (PTH) in the pathogenesis of hepatic osteodystrophy, as well as the predictive significance of biochemical bone markers. Methods: This prospective study included 58 male patients with alcoholic (49) and viral (9) cirrhosis. The concentrations of serum vitamin D-3, PTH, osteocalcin and beta-carboxy-terminal cross-linked telopeptide of type I collagen (beta-CTX) were determined. Bone density was measured by dual energy X-ray absorptiometry in the L1 -L4 spinal segment and the femoral neck. Results: Lower bone mineral density (BMD) was measured in 41 patients (70.7%). There was no significant correlation between PTH and vitamin D3 values and T score in the femoral neck (p=0.51; p=0.063) and lumbar spine (p=0.49; 0.064). Also, no significant correlation was found between the osteocalcin values in lumbar spine BMD (p=0.944) and femoral neck (p=0.161), or with beta-CTX values and BMD in the lumbar spine (p=0.347) and femoral neck (p=0.73). Statistically significant difference was confirmed between the stage A osteocalcin (p=0.000) and beta-CTX (p=0.008) values in relation to advanced stages B and C. Conclusions: PTH and vitamin D3 do not influence the development of hepatic osteodystrophy. In patients with cirrhosis, osteocalcin and beta-CTX are not valid indicators of decreased BMD, but their values correlate with the degree of liver insufficiency.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "The clinical importance of biochemical bone markers in patients with alcoholic and viral liver cirrhosis",
volume = "33",
number = "2",
pages = "149-155",
doi = "10.2478/jomb-2013-0025"
}

Culafić-Vojinović, V., Ćulafić, Đ., Ignjatović, S., Petakov, M., Đurović-Nikolić, M., Vasić, J., Mirković, D., Mijac, D.,& Stulić, M.. (2014). The clinical importance of biochemical bone markers in patients with alcoholic and viral liver cirrhosis. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 33(2), 149-155.
https://doi.org/10.2478/jomb-2013-0025

Culafić-Vojinović V, Ćulafić Đ, Ignjatović S, Petakov M, Đurović-Nikolić M, Vasić J, Mirković D, Mijac D, Stulić M. The clinical importance of biochemical bone markers in patients with alcoholic and viral liver cirrhosis. in Journal of Medical Biochemistry. 2014;33(2):149-155.
doi:10.2478/jomb-2013-0025 .

Culafić-Vojinović, Violeta, Ćulafić, Đorđe, Ignjatović, Svetlana, Petakov, Milan, Đurović-Nikolić, Marina, Vasić, Jelena, Mirković, Duško, Mijac, Dragana, Stulić, Miloš, "The clinical importance of biochemical bone markers in patients with alcoholic and viral liver cirrhosis" in Journal of Medical Biochemistry, 33, no. 2 (2014):149-155,
https://doi.org/10.2478/jomb-2013-0025 . .

TY  - JOUR
AU  - Lezaić, V.
AU  - Mirković, Duško
AU  - Ristić, S.
AU  - Radivojević, Dragana
AU  - Dajak, Marijana
AU  - Naumović, Radomir
AU  - Marinković, Jelena
AU  - Đukanović, Ljubica
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1963
AB  - Purpose. Because no consensus exists regarding the most accurate calculation to estimate glomerular filtration rate (GFR) based on serum creatinine concentrations (sCr) after kidney transplantation, this study sought to assess the potential role of tubular dysfunction on GFR estimates using various equations as well as the effect of pharmacologic blockades on tubular secretion of creatinine on creatinine clearance (ClCr). Methods. Iohexol GFR (mGFR) was performed in 17 stable kidney transplant recipients(R) at >24 months post-transplantation. Their mean age was 48.3 +/- 11.3 years. All R were treated with a calcineurin inhibitor (CNI). At the time of study we measured sCr, 24 hour-ClCr, cystatin C, 24-hour proteinuria, microalbuminuria, FE Na, alfa1-microglobulinuria (alfa1-MG), and CNI concentrations. To block tubular secretion of Cr, recipients were prescribed cimetidine (2400 mg) 2 days before the sCr measurement. Additionally, to exclude dietary influences on sCr, R did not eat meat for 2 days before testing. GFR was estimated using the Modification of Diet in Renal Disease (MDRD), Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI), Cockroft-Gault (C&G), and Cystatin C (Cyst C) GFR equations. Mean kidney graft function over the previous 6 months was used as the contra. Pearson correlation was determined between the differences between mGFR and estimatedGFR: Iohexol minus MDRD, EPI, Cyst C or C&G GFR for paired estimates. The diagnostic accuracy of the eGFRs to detect an mGFR of 60 mL/min/1.73 m(2) was examined by receiver operating characteristic curves. Results. Mean mGFR was 75.2 +/- 35.8 mL/min/1.73 m2. The sCr increased but the 24-hour ClCr, MDRD, EPI, and C&G decreased after vs before cimetidine. The difference was significant for sCr (F = 12.933; P = .002) and MDRD GFR (F = 15.750; P = .001). mGFR was not significantly higher than all pair values of eGFRs, and not significantly lower than 24-hour ClCr before and after cimetidine. However, in comparison to all eGFRs, ClCr after cimetidine most approached mGFR. A significant positive correlation was observed between alfa1-MG and the difference between mGFR and MDRD (before, r = .543 [P = .045]; after cimeticline, 0.568 [P = .034]), EPI (before, r = 0.516 [P = .050]; after cimetidine, r = 0.562 [P = .0361), and ClCr (r = 0.633; P = .016), C&G (P = .581; P = .029) before cimetidine. Accuracy of eGFRs to detect mGFR of 60 mL/min/1.73 m(2) showed EPI GFR before cimetidine to show diagnostic accuracy for patients with GFR >60 mL/min/1.73 m(2) with a sensitivity of 81.8% and a specificity of 71.4%.
PB  - Elsevier Science Inc, New York
T2  - Turkish Journal of Medical Sciences
T1  - Potential Influence of Tubular Dysfunction on the Difference Between Estimated and Measured Glomerular Filtration Rate After Kidney Transplantation
VL  - 45
IS  - 4
SP  - 1651
EP  - 1654
DO  - 10.1016/j.transproceed.2013.02.105
ER  - 

@article{
author = "Lezaić, V. and Mirković, Duško and Ristić, S. and Radivojević, Dragana and Dajak, Marijana and Naumović, Radomir and Marinković, Jelena and Đukanović, Ljubica",
year = "2013",
abstract = "Purpose. Because no consensus exists regarding the most accurate calculation to estimate glomerular filtration rate (GFR) based on serum creatinine concentrations (sCr) after kidney transplantation, this study sought to assess the potential role of tubular dysfunction on GFR estimates using various equations as well as the effect of pharmacologic blockades on tubular secretion of creatinine on creatinine clearance (ClCr). Methods. Iohexol GFR (mGFR) was performed in 17 stable kidney transplant recipients(R) at >24 months post-transplantation. Their mean age was 48.3 +/- 11.3 years. All R were treated with a calcineurin inhibitor (CNI). At the time of study we measured sCr, 24 hour-ClCr, cystatin C, 24-hour proteinuria, microalbuminuria, FE Na, alfa1-microglobulinuria (alfa1-MG), and CNI concentrations. To block tubular secretion of Cr, recipients were prescribed cimetidine (2400 mg) 2 days before the sCr measurement. Additionally, to exclude dietary influences on sCr, R did not eat meat for 2 days before testing. GFR was estimated using the Modification of Diet in Renal Disease (MDRD), Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI), Cockroft-Gault (C&G), and Cystatin C (Cyst C) GFR equations. Mean kidney graft function over the previous 6 months was used as the contra. Pearson correlation was determined between the differences between mGFR and estimatedGFR: Iohexol minus MDRD, EPI, Cyst C or C&G GFR for paired estimates. The diagnostic accuracy of the eGFRs to detect an mGFR of 60 mL/min/1.73 m(2) was examined by receiver operating characteristic curves. Results. Mean mGFR was 75.2 +/- 35.8 mL/min/1.73 m2. The sCr increased but the 24-hour ClCr, MDRD, EPI, and C&G decreased after vs before cimetidine. The difference was significant for sCr (F = 12.933; P = .002) and MDRD GFR (F = 15.750; P = .001). mGFR was not significantly higher than all pair values of eGFRs, and not significantly lower than 24-hour ClCr before and after cimetidine. However, in comparison to all eGFRs, ClCr after cimetidine most approached mGFR. A significant positive correlation was observed between alfa1-MG and the difference between mGFR and MDRD (before, r = .543 [P = .045]; after cimeticline, 0.568 [P = .034]), EPI (before, r = 0.516 [P = .050]; after cimetidine, r = 0.562 [P = .0361), and ClCr (r = 0.633; P = .016), C&G (P = .581; P = .029) before cimetidine. Accuracy of eGFRs to detect mGFR of 60 mL/min/1.73 m(2) showed EPI GFR before cimetidine to show diagnostic accuracy for patients with GFR >60 mL/min/1.73 m(2) with a sensitivity of 81.8% and a specificity of 71.4%.",
publisher = "Elsevier Science Inc, New York",
journal = "Turkish Journal of Medical Sciences",
title = "Potential Influence of Tubular Dysfunction on the Difference Between Estimated and Measured Glomerular Filtration Rate After Kidney Transplantation",
volume = "45",
number = "4",
pages = "1651-1654",
doi = "10.1016/j.transproceed.2013.02.105"
}

Lezaić, V., Mirković, D., Ristić, S., Radivojević, D., Dajak, M., Naumović, R., Marinković, J.,& Đukanović, L.. (2013). Potential Influence of Tubular Dysfunction on the Difference Between Estimated and Measured Glomerular Filtration Rate After Kidney Transplantation. in Turkish Journal of Medical Sciences
Elsevier Science Inc, New York., 45(4), 1651-1654.
https://doi.org/10.1016/j.transproceed.2013.02.105

Lezaić V, Mirković D, Ristić S, Radivojević D, Dajak M, Naumović R, Marinković J, Đukanović L. Potential Influence of Tubular Dysfunction on the Difference Between Estimated and Measured Glomerular Filtration Rate After Kidney Transplantation. in Turkish Journal of Medical Sciences. 2013;45(4):1651-1654.
doi:10.1016/j.transproceed.2013.02.105 .

Lezaić, V., Mirković, Duško, Ristić, S., Radivojević, Dragana, Dajak, Marijana, Naumović, Radomir, Marinković, Jelena, Đukanović, Ljubica, "Potential Influence of Tubular Dysfunction on the Difference Between Estimated and Measured Glomerular Filtration Rate After Kidney Transplantation" in Turkish Journal of Medical Sciences, 45, no. 4 (2013):1651-1654,
https://doi.org/10.1016/j.transproceed.2013.02.105 . .

TY  - CONF
AU  - Lezaić, V
AU  - Mirković, Duško
AU  - Ristić, S.
AU  - Radivojević, Dragana
AU  - Dajak, Marijana
AU  - Naumović, Radomir
AU  - Đukanović, Ljubica
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1658
PB  - Lippincott Williams & Wilkins, Philadelphia
C3  - Transplantation Proceedings
T1  - Potential Influence of Tubular Dysfunction in Agreement of Estimated and Measured Glomerular Fultration Rate after Kidney Transplantation
VL  - 94
IS  - 10
SP  - 873
EP  - 873
DO  - 10.1097/00007890-201211271-01718
ER  - 

@conference{
author = "Lezaić, V and Mirković, Duško and Ristić, S. and Radivojević, Dragana and Dajak, Marijana and Naumović, Radomir and Đukanović, Ljubica",
year = "2012",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Transplantation Proceedings",
title = "Potential Influence of Tubular Dysfunction in Agreement of Estimated and Measured Glomerular Fultration Rate after Kidney Transplantation",
volume = "94",
number = "10",
pages = "873-873",
doi = "10.1097/00007890-201211271-01718"
}

Lezaić, V., Mirković, D., Ristić, S., Radivojević, D., Dajak, M., Naumović, R.,& Đukanović, L.. (2012). Potential Influence of Tubular Dysfunction in Agreement of Estimated and Measured Glomerular Fultration Rate after Kidney Transplantation. in Transplantation Proceedings
Lippincott Williams & Wilkins, Philadelphia., 94(10), 873-873.
https://doi.org/10.1097/00007890-201211271-01718

Lezaić V, Mirković D, Ristić S, Radivojević D, Dajak M, Naumović R, Đukanović L. Potential Influence of Tubular Dysfunction in Agreement of Estimated and Measured Glomerular Fultration Rate after Kidney Transplantation. in Transplantation Proceedings. 2012;94(10):873-873.
doi:10.1097/00007890-201211271-01718 .

Lezaić, V, Mirković, Duško, Ristić, S., Radivojević, Dragana, Dajak, Marijana, Naumović, Radomir, Đukanović, Ljubica, "Potential Influence of Tubular Dysfunction in Agreement of Estimated and Measured Glomerular Fultration Rate after Kidney Transplantation" in Transplantation Proceedings, 94, no. 10 (2012):873-873,
https://doi.org/10.1097/00007890-201211271-01718 . .

TY  - JOUR
AU  - Jovičić, Snežana
AU  - Ignjatović, Svetlana
AU  - Kangrga, Ranka
AU  - Beletić, Anđelo
AU  - Mirković, Duško
AU  - Majkić-Singh, Nada
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1656
AB  - Determination of 25-hydroxyvitamin D [25(OH)D] represents a unique challenge, considering its lipophilic nature. Considering the widespread prevalence of vitamin D deficiency, which leads to increasing number of requests for 25(OH)D determination, immunoassay measurements adjusted to automated analyzers are being developed. Because of the variability among assays, it is often difficult to monitor vitamin D status and supplementation. The aim of this study was to compare the results of two immunoassays with high performance liquid chromatography with ultraviolet detection (HPLC-UV). Also, the aim was to estimate vitamin D status, since up to date the prevalence of vitamin D deficiency in Serbia was not examined. We have evaluated analytical characteristics of two automated immunoassays for 25(OH)D determination, from Roche (Cobas® e601) and Abbott (Architect). For comparison studies we used HPLC analysis of 25-(OH)-Vitamin D3/D2 from Chromsystems (Waters isocratic system). In order to estimate vitamin D status in general population, we have searched the database of the laboratory information system and analyzed the data from 533 patients whose 25(OH)D was determined together with intact parathyroid hormone (iPTH). For imprecision assessment, four serum pools were prepared with following 25(OH)D concentrations: 35 nmol/L, ?50 nmol/L, ?75 nmol/L and ?125 nmol/L. Obtai ned CVs for Roche method were 1.5-2.8% for within-run and 4.0-6.7% for between-run imprecision. For Abbott method, CVs were 0.7-4.4% for withinrun and 3.8-7.2% for between-run imprecision. Inaccuracy was analyzed with commercial control sera. Obtained deviations from target value were 2.1% for Roche assay and 1.3-1.5% for Abbott method, and were not statistically significant (P>0.05). Comparison of Roche and HPLC-UV methods using Passing-Bablok regression analysis gave the following equation for the regression line y=0.937x+9.518 (r=0.739; n=97) and the regression line equation from the comparison of Abbott and HPLC-UV methods was y=0.745x+10.343 (r=0.793; n=97). Mean difference and SD for Bland-Altman plot were -4.5 nmol/L and 21.75 nmo/L, respectively for Roche method and 6.4 nmol/L and 18.8 nmol/L, respectively for Abbott. Statistical analysis (Chi-square test) of frequency distribution among different vitamin D status categories ( lt 25 nmol/L severe deficiency, 25-50 nmol/L deficiency, 50-75 nmol/L insufficiency and >75 nmol/L sufficiency) showed that the frequency distribution obtained with Abbott method was significantly different from the distribution of the HPLC results, in contrast to Roche results frequency distribution which did not differ significantly. Also, statistical analysis of the agreement between the three methods for each vitamin D status category showed that results of both Roche and Abbott methods were significantly higher than HPLC in the two deficiency categories (P=0.005 for Roche, P=0.0407 for Abbott), and in the sufficiency category Abbott method significantly underestimated concentration of 25(OH)D compared to HPLC results (P lt 0.0001). Median population values of 25(OH)D and iPTH were 41.8 nmol/L and 76.6 ng/L, respectively. ANOVA analyses showed significant (P lt 0.05) decrease in iPTH and Ca2+ concentrations across the 25(OH)D concentration categories. Stepwise multiple linear regression analysis indicated independent correlation of iPTH with 25(OH)D concentration (b=-0.290, P=0.0008). Also, one-way ANOVA with Student-Newman-Keuls test demonstrated that 25(OH)D concentrations measured in summer and autumn were significantly (P lt 0.001) higher compared to those determined in winter and spring. Despite acceptable imprecision and inaccuracy of both examined methods, results obtained with them did not correlate well with HPLC-UV (r lt 0.9), which was used as a reference. However, methods showed satisfactory ability to classify patients into vitamin D status categories, which is important for diagnosis of vitamin D deficiency and therapy follow-up. About two thirds (68.5%) of the examined population had vitamin D deficiency (25(OH)D lt 50 nmol/L) and only 8% had sufficient 25(OH)D concentration (>75 nmol/L).
AB  - Određivanje 25-hidroksivitamina D [25(OH)D] predstavlja jedinstven izazov, s obzirom da je visoko lipofilno jedinjenje. Visoka prevalencija deficijencije vitamina D uzrok je povećanja broja zahteva za određivanjem 25(OH)D, zbog čega se razvijaju imunohemijske metode prilagođene automatizovanim sistemima. Često je teško pratiti status vitamina D i suplementaciju zbog varijabilnosti između testova. Cilj ove studije bio je da se uporede rezultati dve imunohemijske metode sa tečnom hromatografijom visoke efikasnosti sa detekcijom u ultraljubičastom delu spektra (HPLC-UV). Takođe, cilj je bio i procena statusa vitamina D, pošto do sada nije ispitivana prevalencija deficijencije vitamina D u Srbiji. Ispitivane su karakteristike dve imunohemijske metode za određivanje 25(OH)D, proizvođača Roche (analizator Cobas® e601) i Abbott (na analizatoru Architect). Metode su poređene sa rezultatima HPLC analize korišćenjem 25-(OH)-Vitamin D3/D2 reagenasa firme Chromsystems (Waters izokratski sistem). Da bi se procenio status vitamina D u opštoj populaciji, pretražena je baza podataka laboratorijskog informacionog sistema i analizirani su rezultati 533 pacijenata kojima je određen 25(OH)D zajedno sa intaktnim paratiroidnim hormonom (iPTH). Pripremljena su četiri serumska pool-a sa koncentracijama 25(OH)D ? 35 nmol/L, ?50 nmol/L, ?75 nmol/L i ?125 nmol/L za procenu nepreciznosti imunohemijskih određivanja. Dobijeni koeficijenti varijacije za Roche metodu su se kretali u opsegu 1,5-2,8% u seriji i 4,0-6,7% između serija. Za Abbott metodu su koficijenti varijacije iznosili 0,7-4,4% u seriji i 3,8-7,2% između serija. Netačnost je ispitivana pomoću komercijalnih kontrolnih uzoraka. Dobijena odstupanja od deklarisane vrednosti su iznosila 2,1% za Roche i 1,3-1,5% za Abbott, i nisu bila statistički značajna (P>0,05). Poređenjem Roche i HPLC-UV metoda pomoću Passing-Bablok regresione analize dobijena je sledeća regresiona jednačina y=0,937x+9,518 (r=0,739; n=97), dok regresiona jednačina dobijena poređenjem Abbott i HPLC-UV metoda glasi y=0,745x+10,343 (r=0,793; n=97). Srednja vrednost razlika na Bland-Altman dijagramu razlika i standardna devijacija su iznosile -4,5 nmol/L i 21,75 nmo/L, redom, za Roche metodu i 6,4 nmol/L i 18,8 nmol/L, re dom, za Abbott metodu. Statistička analiza (Chi-kvadrat test) distribucije frekvencija među različitim kategorijama statusa vitamina D ( lt 25 nmol/L teška deficijencija, 25-50 nmol/L deficijencija, 50-75 nmol/L insuficijencija i >75 nmol/L preporučena koncentracija) je pokazala da je distribucija frekvencija dobijena Abbott metodom značajno različita od distribucije HPLC rezultata, za razliku od ras po dele frekvencija dobijene Roche metodom koja se nije značajno razlikovala. Takođe, statistička analiza slaganja između ispitivane tri metode u svakoj od kategorija statusa vitamina D je pokazala da su rezultati i Roche i Abbott metoda značajno veći od HPLC-UV u kategorijama deficijencije vitamina D (P=0,005 za Roche; P=0,0407 za Abbott), i u kategoriji sa preporučenom koncentracijom vitamina D Abbott metoda je značajno potcenjivala koncentraciju 25(OH)D u poređenju sa HPLC rezultatima (P lt 0,0001). Medijana za 25(OH)D u ispitivanoj populaciji bila je 41,8 nmol/L, i 76,6 za iPTH. ANOVA analiza je pokazala značajan pad (P lt 0,05) koncentracija iPTH i jonizovanog kalcijuma između kategorija koncentracija 25(OH)D. Multiplomlinearnom regresionom analizom utvrđena je ne zavisna korelacija između koncentracija iPTH i 25(OH)D (b =-0,290; P=0,0008). Takođe, ANOVA za jedan kriterijum klasifikacije sa Student-Newman-Keuls testom je pokazala da su koncentracije 25(OH)D određene u leto i jesen značajno više (P lt 0,001) u poređenju sa onima određenim u zimu ili proleće. Uprkos prihvatljivoj nepreciznosti i netačnosti obe ispitivane imunohemijske metode, dobijeni rezultati nisu u zadovoljavajućoj korelaciji sa HPLC-UV metodom (r lt 0,9), koja je korišćena kao referentna u ovom slučaju. Uprkos ovoj činjenici, metode su pokazale zadovoljavajuću sposobnost klasifikacije pacijenata u kategorije statusa vitamina D, što je važno za dijagnozu deficijencije vitamina D i praćenje terapije. Oko dve trećine (68,5%) ispitivane populacije je imalo deficijenciju vitamina D (25(OH)D lt 50 nmol/L) i samo 8% je imalo preporučenu koncentraciju 25(OH)D (>75 nmol/L).
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience
T1  - Poređenje tri različite metode za određivanje 25(OH)-vitamina D i statusa vitamina D u opštoj populaciji - srpsko iskustvo
VL  - 31
IS  - 4
SP  - 347
EP  - 357
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1656
ER  - 

@article{
author = "Jovičić, Snežana and Ignjatović, Svetlana and Kangrga, Ranka and Beletić, Anđelo and Mirković, Duško and Majkić-Singh, Nada",
year = "2012",
abstract = "Determination of 25-hydroxyvitamin D [25(OH)D] represents a unique challenge, considering its lipophilic nature. Considering the widespread prevalence of vitamin D deficiency, which leads to increasing number of requests for 25(OH)D determination, immunoassay measurements adjusted to automated analyzers are being developed. Because of the variability among assays, it is often difficult to monitor vitamin D status and supplementation. The aim of this study was to compare the results of two immunoassays with high performance liquid chromatography with ultraviolet detection (HPLC-UV). Also, the aim was to estimate vitamin D status, since up to date the prevalence of vitamin D deficiency in Serbia was not examined. We have evaluated analytical characteristics of two automated immunoassays for 25(OH)D determination, from Roche (Cobas® e601) and Abbott (Architect). For comparison studies we used HPLC analysis of 25-(OH)-Vitamin D3/D2 from Chromsystems (Waters isocratic system). In order to estimate vitamin D status in general population, we have searched the database of the laboratory information system and analyzed the data from 533 patients whose 25(OH)D was determined together with intact parathyroid hormone (iPTH). For imprecision assessment, four serum pools were prepared with following 25(OH)D concentrations: 35 nmol/L, ?50 nmol/L, ?75 nmol/L and ?125 nmol/L. Obtai ned CVs for Roche method were 1.5-2.8% for within-run and 4.0-6.7% for between-run imprecision. For Abbott method, CVs were 0.7-4.4% for withinrun and 3.8-7.2% for between-run imprecision. Inaccuracy was analyzed with commercial control sera. Obtained deviations from target value were 2.1% for Roche assay and 1.3-1.5% for Abbott method, and were not statistically significant (P>0.05). Comparison of Roche and HPLC-UV methods using Passing-Bablok regression analysis gave the following equation for the regression line y=0.937x+9.518 (r=0.739; n=97) and the regression line equation from the comparison of Abbott and HPLC-UV methods was y=0.745x+10.343 (r=0.793; n=97). Mean difference and SD for Bland-Altman plot were -4.5 nmol/L and 21.75 nmo/L, respectively for Roche method and 6.4 nmol/L and 18.8 nmol/L, respectively for Abbott. Statistical analysis (Chi-square test) of frequency distribution among different vitamin D status categories ( lt 25 nmol/L severe deficiency, 25-50 nmol/L deficiency, 50-75 nmol/L insufficiency and >75 nmol/L sufficiency) showed that the frequency distribution obtained with Abbott method was significantly different from the distribution of the HPLC results, in contrast to Roche results frequency distribution which did not differ significantly. Also, statistical analysis of the agreement between the three methods for each vitamin D status category showed that results of both Roche and Abbott methods were significantly higher than HPLC in the two deficiency categories (P=0.005 for Roche, P=0.0407 for Abbott), and in the sufficiency category Abbott method significantly underestimated concentration of 25(OH)D compared to HPLC results (P lt 0.0001). Median population values of 25(OH)D and iPTH were 41.8 nmol/L and 76.6 ng/L, respectively. ANOVA analyses showed significant (P lt 0.05) decrease in iPTH and Ca2+ concentrations across the 25(OH)D concentration categories. Stepwise multiple linear regression analysis indicated independent correlation of iPTH with 25(OH)D concentration (b=-0.290, P=0.0008). Also, one-way ANOVA with Student-Newman-Keuls test demonstrated that 25(OH)D concentrations measured in summer and autumn were significantly (P lt 0.001) higher compared to those determined in winter and spring. Despite acceptable imprecision and inaccuracy of both examined methods, results obtained with them did not correlate well with HPLC-UV (r lt 0.9), which was used as a reference. However, methods showed satisfactory ability to classify patients into vitamin D status categories, which is important for diagnosis of vitamin D deficiency and therapy follow-up. About two thirds (68.5%) of the examined population had vitamin D deficiency (25(OH)D lt 50 nmol/L) and only 8% had sufficient 25(OH)D concentration (>75 nmol/L)., Određivanje 25-hidroksivitamina D [25(OH)D] predstavlja jedinstven izazov, s obzirom da je visoko lipofilno jedinjenje. Visoka prevalencija deficijencije vitamina D uzrok je povećanja broja zahteva za određivanjem 25(OH)D, zbog čega se razvijaju imunohemijske metode prilagođene automatizovanim sistemima. Često je teško pratiti status vitamina D i suplementaciju zbog varijabilnosti između testova. Cilj ove studije bio je da se uporede rezultati dve imunohemijske metode sa tečnom hromatografijom visoke efikasnosti sa detekcijom u ultraljubičastom delu spektra (HPLC-UV). Takođe, cilj je bio i procena statusa vitamina D, pošto do sada nije ispitivana prevalencija deficijencije vitamina D u Srbiji. Ispitivane su karakteristike dve imunohemijske metode za određivanje 25(OH)D, proizvođača Roche (analizator Cobas® e601) i Abbott (na analizatoru Architect). Metode su poređene sa rezultatima HPLC analize korišćenjem 25-(OH)-Vitamin D3/D2 reagenasa firme Chromsystems (Waters izokratski sistem). Da bi se procenio status vitamina D u opštoj populaciji, pretražena je baza podataka laboratorijskog informacionog sistema i analizirani su rezultati 533 pacijenata kojima je određen 25(OH)D zajedno sa intaktnim paratiroidnim hormonom (iPTH). Pripremljena su četiri serumska pool-a sa koncentracijama 25(OH)D ? 35 nmol/L, ?50 nmol/L, ?75 nmol/L i ?125 nmol/L za procenu nepreciznosti imunohemijskih određivanja. Dobijeni koeficijenti varijacije za Roche metodu su se kretali u opsegu 1,5-2,8% u seriji i 4,0-6,7% između serija. Za Abbott metodu su koficijenti varijacije iznosili 0,7-4,4% u seriji i 3,8-7,2% između serija. Netačnost je ispitivana pomoću komercijalnih kontrolnih uzoraka. Dobijena odstupanja od deklarisane vrednosti su iznosila 2,1% za Roche i 1,3-1,5% za Abbott, i nisu bila statistički značajna (P>0,05). Poređenjem Roche i HPLC-UV metoda pomoću Passing-Bablok regresione analize dobijena je sledeća regresiona jednačina y=0,937x+9,518 (r=0,739; n=97), dok regresiona jednačina dobijena poređenjem Abbott i HPLC-UV metoda glasi y=0,745x+10,343 (r=0,793; n=97). Srednja vrednost razlika na Bland-Altman dijagramu razlika i standardna devijacija su iznosile -4,5 nmol/L i 21,75 nmo/L, redom, za Roche metodu i 6,4 nmol/L i 18,8 nmol/L, re dom, za Abbott metodu. Statistička analiza (Chi-kvadrat test) distribucije frekvencija među različitim kategorijama statusa vitamina D ( lt 25 nmol/L teška deficijencija, 25-50 nmol/L deficijencija, 50-75 nmol/L insuficijencija i >75 nmol/L preporučena koncentracija) je pokazala da je distribucija frekvencija dobijena Abbott metodom značajno različita od distribucije HPLC rezultata, za razliku od ras po dele frekvencija dobijene Roche metodom koja se nije značajno razlikovala. Takođe, statistička analiza slaganja između ispitivane tri metode u svakoj od kategorija statusa vitamina D je pokazala da su rezultati i Roche i Abbott metoda značajno veći od HPLC-UV u kategorijama deficijencije vitamina D (P=0,005 za Roche; P=0,0407 za Abbott), i u kategoriji sa preporučenom koncentracijom vitamina D Abbott metoda je značajno potcenjivala koncentraciju 25(OH)D u poređenju sa HPLC rezultatima (P lt 0,0001). Medijana za 25(OH)D u ispitivanoj populaciji bila je 41,8 nmol/L, i 76,6 za iPTH. ANOVA analiza je pokazala značajan pad (P lt 0,05) koncentracija iPTH i jonizovanog kalcijuma između kategorija koncentracija 25(OH)D. Multiplomlinearnom regresionom analizom utvrđena je ne zavisna korelacija između koncentracija iPTH i 25(OH)D (b =-0,290; P=0,0008). Takođe, ANOVA za jedan kriterijum klasifikacije sa Student-Newman-Keuls testom je pokazala da su koncentracije 25(OH)D određene u leto i jesen značajno više (P lt 0,001) u poređenju sa onima određenim u zimu ili proleće. Uprkos prihvatljivoj nepreciznosti i netačnosti obe ispitivane imunohemijske metode, dobijeni rezultati nisu u zadovoljavajućoj korelaciji sa HPLC-UV metodom (r lt 0,9), koja je korišćena kao referentna u ovom slučaju. Uprkos ovoj činjenici, metode su pokazale zadovoljavajuću sposobnost klasifikacije pacijenata u kategorije statusa vitamina D, što je važno za dijagnozu deficijencije vitamina D i praćenje terapije. Oko dve trećine (68,5%) ispitivane populacije je imalo deficijenciju vitamina D (25(OH)D lt 50 nmol/L) i samo 8% je imalo preporučenu koncentraciju 25(OH)D (>75 nmol/L).",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience, Poređenje tri različite metode za određivanje 25(OH)-vitamina D i statusa vitamina D u opštoj populaciji - srpsko iskustvo",
volume = "31",
number = "4",
pages = "347-357",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1656"
}

Jovičić, S., Ignjatović, S., Kangrga, R., Beletić, A., Mirković, D.,& Majkić-Singh, N.. (2012). Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 31(4), 347-357.
https://hdl.handle.net/21.15107/rcub_farfar_1656

Jovičić S, Ignjatović S, Kangrga R, Beletić A, Mirković D, Majkić-Singh N. Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience. in Journal of Medical Biochemistry. 2012;31(4):347-357.
https://hdl.handle.net/21.15107/rcub_farfar_1656 .

Jovičić, Snežana, Ignjatović, Svetlana, Kangrga, Ranka, Beletić, Anđelo, Mirković, Duško, Majkić-Singh, Nada, "Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience" in Journal of Medical Biochemistry, 31, no. 4 (2012):347-357,
https://hdl.handle.net/21.15107/rcub_farfar_1656 .

TY  - CONF
AU  - Beletić, Anđelo
AU  - Đorđević, Valentina
AU  - Canić, I.
AU  - Kocica, T.
AU  - Kuzmanović, I.
AU  - Golubović, Milka
AU  - Mirković, Duško
AU  - Radojković, Dragica
AU  - Majkić-Singh, Nada
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1531
PB  - Walter de Gruyter & Co, Berlin
C3  - Clinical Chemistry and Laboratory Medicine
T1  - Experiences in simultaneous detection of factor v leiden, factor ii g20210a, mthfr c677t and mthfr a1298c mutations in patients with thrombophilia
VL  - 49
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1531
ER  - 

@conference{
author = "Beletić, Anđelo and Đorđević, Valentina and Canić, I. and Kocica, T. and Kuzmanović, I. and Golubović, Milka and Mirković, Duško and Radojković, Dragica and Majkić-Singh, Nada",
year = "2011",
publisher = "Walter de Gruyter & Co, Berlin",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Experiences in simultaneous detection of factor v leiden, factor ii g20210a, mthfr c677t and mthfr a1298c mutations in patients with thrombophilia",
volume = "49",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1531"
}

Beletić, A., Đorđević, V., Canić, I., Kocica, T., Kuzmanović, I., Golubović, M., Mirković, D., Radojković, D.,& Majkić-Singh, N.. (2011). Experiences in simultaneous detection of factor v leiden, factor ii g20210a, mthfr c677t and mthfr a1298c mutations in patients with thrombophilia. in Clinical Chemistry and Laboratory Medicine
Walter de Gruyter & Co, Berlin., 49.
https://hdl.handle.net/21.15107/rcub_farfar_1531

Beletić A, Đorđević V, Canić I, Kocica T, Kuzmanović I, Golubović M, Mirković D, Radojković D, Majkić-Singh N. Experiences in simultaneous detection of factor v leiden, factor ii g20210a, mthfr c677t and mthfr a1298c mutations in patients with thrombophilia. in Clinical Chemistry and Laboratory Medicine. 2011;49.
https://hdl.handle.net/21.15107/rcub_farfar_1531 .

Beletić, Anđelo, Đorđević, Valentina, Canić, I., Kocica, T., Kuzmanović, I., Golubović, Milka, Mirković, Duško, Radojković, Dragica, Majkić-Singh, Nada, "Experiences in simultaneous detection of factor v leiden, factor ii g20210a, mthfr c677t and mthfr a1298c mutations in patients with thrombophilia" in Clinical Chemistry and Laboratory Medicine, 49 (2011),
https://hdl.handle.net/21.15107/rcub_farfar_1531 .

TY  - CONF
AU  - Mirković, Duško
AU  - Ćulafić, Đorđe
AU  - Rudić, J. S.
AU  - Beletić, Anđelo
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1530
PB  - Walter de Gruyter & Co, Berlin
C3  - Clinical Chemistry and Laboratory Medicine
T1  - Importance of serotonin determination in patients with esophageal and gastric fundus varices
VL  - 49
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1530
ER  - 

@conference{
author = "Mirković, Duško and Ćulafić, Đorđe and Rudić, J. S. and Beletić, Anđelo",
year = "2011",
publisher = "Walter de Gruyter & Co, Berlin",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Importance of serotonin determination in patients with esophageal and gastric fundus varices",
volume = "49",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1530"
}

Mirković, D., Ćulafić, Đ., Rudić, J. S.,& Beletić, A.. (2011). Importance of serotonin determination in patients with esophageal and gastric fundus varices. in Clinical Chemistry and Laboratory Medicine
Walter de Gruyter & Co, Berlin., 49.
https://hdl.handle.net/21.15107/rcub_farfar_1530

Mirković D, Ćulafić Đ, Rudić JS, Beletić A. Importance of serotonin determination in patients with esophageal and gastric fundus varices. in Clinical Chemistry and Laboratory Medicine. 2011;49.
https://hdl.handle.net/21.15107/rcub_farfar_1530 .

Mirković, Duško, Ćulafić, Đorđe, Rudić, J. S., Beletić, Anđelo, "Importance of serotonin determination in patients with esophageal and gastric fundus varices" in Clinical Chemistry and Laboratory Medicine, 49 (2011),
https://hdl.handle.net/21.15107/rcub_farfar_1530 .

TY  - JOUR
AU  - Rudić, Jelena S.
AU  - Ćulafić, Đorđe
AU  - Mirković, Duško
AU  - Jesić, Rada S.
AU  - Krstić, Miodrag N.
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1365
AB  - AIM: To determine the effect of free serotonin concentrations in plasma on development of esophageal and gastric fundal varices. METHODS: This prospective study included 33 patients with liver cirrhosis and 24 healthy controls. Ultrasonography and measurement of serotonin concentration in plasma were carried out in both groups of subjects. The upper fiber panendoscopy was performed only in patients with liver cirrhosis. RESULTS: The mean plasma free serotonin levels were much higher in liver cirrhosis patients than in healthy controls (219.0 +/- 24.2 nmol/L vs 65.4 +/- 18.7 nmol/L, P  lt  0.0001). There was no significant correlation be-tween serotonin concentration in plasma and the size of the esophageal varices according to Spearman coefficient of correlation (r(s) = -0.217, P > 0.05). However, the correlation of plasma serotonin concentration and gastric fundal varices was highly significant (r(s) = -0.601, P  lt  0.01). CONCLUSION: Free serotonin is significant in pathogenesis of portal hypertension especially in development of fundal varices, indicating the clinical value of serotonergic receptor blockers in these patients.
PB  - Baishideng Publ Grp Co Ltd, Beijing
T2  - World Journal of Microbiology & Biotechnology
T1  - Role of serotonin in development of esophageal and gastric fundal varices
VL  - 16
IS  - 48
SP  - 6135
EP  - 6138
DO  - 10.3748/wjg.v16.i48.6135
ER  - 

@article{
author = "Rudić, Jelena S. and Ćulafić, Đorđe and Mirković, Duško and Jesić, Rada S. and Krstić, Miodrag N.",
year = "2010",
abstract = "AIM: To determine the effect of free serotonin concentrations in plasma on development of esophageal and gastric fundal varices. METHODS: This prospective study included 33 patients with liver cirrhosis and 24 healthy controls. Ultrasonography and measurement of serotonin concentration in plasma were carried out in both groups of subjects. The upper fiber panendoscopy was performed only in patients with liver cirrhosis. RESULTS: The mean plasma free serotonin levels were much higher in liver cirrhosis patients than in healthy controls (219.0 +/- 24.2 nmol/L vs 65.4 +/- 18.7 nmol/L, P  lt  0.0001). There was no significant correlation be-tween serotonin concentration in plasma and the size of the esophageal varices according to Spearman coefficient of correlation (r(s) = -0.217, P > 0.05). However, the correlation of plasma serotonin concentration and gastric fundal varices was highly significant (r(s) = -0.601, P  lt  0.01). CONCLUSION: Free serotonin is significant in pathogenesis of portal hypertension especially in development of fundal varices, indicating the clinical value of serotonergic receptor blockers in these patients.",
publisher = "Baishideng Publ Grp Co Ltd, Beijing",
journal = "World Journal of Microbiology & Biotechnology",
title = "Role of serotonin in development of esophageal and gastric fundal varices",
volume = "16",
number = "48",
pages = "6135-6138",
doi = "10.3748/wjg.v16.i48.6135"
}

Rudić, J. S., Ćulafić, Đ., Mirković, D., Jesić, R. S.,& Krstić, M. N.. (2010). Role of serotonin in development of esophageal and gastric fundal varices. in World Journal of Microbiology & Biotechnology
Baishideng Publ Grp Co Ltd, Beijing., 16(48), 6135-6138.
https://doi.org/10.3748/wjg.v16.i48.6135

Rudić JS, Ćulafić Đ, Mirković D, Jesić RS, Krstić MN. Role of serotonin in development of esophageal and gastric fundal varices. in World Journal of Microbiology & Biotechnology. 2010;16(48):6135-6138.
doi:10.3748/wjg.v16.i48.6135 .

Rudić, Jelena S., Ćulafić, Đorđe, Mirković, Duško, Jesić, Rada S., Krstić, Miodrag N., "Role of serotonin in development of esophageal and gastric fundal varices" in World Journal of Microbiology & Biotechnology, 16, no. 48 (2010):6135-6138,
https://doi.org/10.3748/wjg.v16.i48.6135 . .

TY  - JOUR
AU  - Radovanović, N.
AU  - Antonijević, N.
AU  - Beletić, Anđelo
AU  - Perunicić, J.
AU  - Kocica, Mladen J.
AU  - Mirković, Duško
AU  - Lacković, Vesna
AU  - Lacković, Milena
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1326
AB  - Investigation of hyperhomocysteinemia (HHcy) as an important risk factor for pulmonary thromboembolism (PTE), which represents a most dangerous consequence of a unique phenomenon of venous thromboembolism which still suffers from sometimes conflicting or inadequately clarified results. The role of homocysteine in the clinical manifestation of this life-threatening disease and its treatment (in which any further information may be decisive) requires detailed examination. The purpose of this study is to determine the differences in HHcy incidence and homocysteinemia levels between patients with PTE and healthy persons. The study enrolled 70 patients with PTE and 50 healthy persons. Homocysteine was measured using the HPLC method with fluorescent detection and HHcy was defined as homocysteinemia above 12 mu mol/L. Statistical analyses included chi-square and Mann Whitney U tests. The median homocysteinemia value was significantly higher (p=0.017) in the patients (12.10 mu mol/L) than in the controls (10.35 mu mol/L). The comparison of HHcy incidence between the patients (51.5%) and controls (30%) revealed a significant difference (p=0.021). In patients, homocysteinemia was significantly higher (p=0.002) in men (14.05 mu mol/L) than in women (10.01 mu mol/L) HHcy was present in 67.6% of men with PTE, which was significantly higher (p=0.006) than the incidence in women with PTE (33.3%). Healthy males had significantly higher (p=0.001) homocysteinemia (12.54 mu mol/L) than healthy females (9.4 mu mol/L). A significant difference (p=0.031) was observed between the incidences of HHcy in healthy males (44.0%) and healthy females (16.0%). We conclude that the incidence of hyperhomocysteinemia and homocysteinemia are significantly higher in all the patients compared with de healthy persons, as well as in both healthy males and males with PTE compared with healthy females and female patients. This indicates that HHcy findings in PE are likely to have a clinical importance.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Hyperhomocysteinemia in patients with pulmonary embolism
VL  - 62
IS  - 4
SP  - 907
EP  - 914
DO  - 10.2298/ABS1004907R
ER  - 

@article{
author = "Radovanović, N. and Antonijević, N. and Beletić, Anđelo and Perunicić, J. and Kocica, Mladen J. and Mirković, Duško and Lacković, Vesna and Lacković, Milena",
year = "2010",
abstract = "Investigation of hyperhomocysteinemia (HHcy) as an important risk factor for pulmonary thromboembolism (PTE), which represents a most dangerous consequence of a unique phenomenon of venous thromboembolism which still suffers from sometimes conflicting or inadequately clarified results. The role of homocysteine in the clinical manifestation of this life-threatening disease and its treatment (in which any further information may be decisive) requires detailed examination. The purpose of this study is to determine the differences in HHcy incidence and homocysteinemia levels between patients with PTE and healthy persons. The study enrolled 70 patients with PTE and 50 healthy persons. Homocysteine was measured using the HPLC method with fluorescent detection and HHcy was defined as homocysteinemia above 12 mu mol/L. Statistical analyses included chi-square and Mann Whitney U tests. The median homocysteinemia value was significantly higher (p=0.017) in the patients (12.10 mu mol/L) than in the controls (10.35 mu mol/L). The comparison of HHcy incidence between the patients (51.5%) and controls (30%) revealed a significant difference (p=0.021). In patients, homocysteinemia was significantly higher (p=0.002) in men (14.05 mu mol/L) than in women (10.01 mu mol/L) HHcy was present in 67.6% of men with PTE, which was significantly higher (p=0.006) than the incidence in women with PTE (33.3%). Healthy males had significantly higher (p=0.001) homocysteinemia (12.54 mu mol/L) than healthy females (9.4 mu mol/L). A significant difference (p=0.031) was observed between the incidences of HHcy in healthy males (44.0%) and healthy females (16.0%). We conclude that the incidence of hyperhomocysteinemia and homocysteinemia are significantly higher in all the patients compared with de healthy persons, as well as in both healthy males and males with PTE compared with healthy females and female patients. This indicates that HHcy findings in PE are likely to have a clinical importance.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Hyperhomocysteinemia in patients with pulmonary embolism",
volume = "62",
number = "4",
pages = "907-914",
doi = "10.2298/ABS1004907R"
}

Radovanović, N., Antonijević, N., Beletić, A., Perunicić, J., Kocica, M. J., Mirković, D., Lacković, V.,& Lacković, M.. (2010). Hyperhomocysteinemia in patients with pulmonary embolism. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(4), 907-914.
https://doi.org/10.2298/ABS1004907R

Radovanović N, Antonijević N, Beletić A, Perunicić J, Kocica MJ, Mirković D, Lacković V, Lacković M. Hyperhomocysteinemia in patients with pulmonary embolism. in Archives of Biological Sciences. 2010;62(4):907-914.
doi:10.2298/ABS1004907R .

Radovanović, N., Antonijević, N., Beletić, Anđelo, Perunicić, J., Kocica, Mladen J., Mirković, Duško, Lacković, Vesna, Lacković, Milena, "Hyperhomocysteinemia in patients with pulmonary embolism" in Archives of Biological Sciences, 62, no. 4 (2010):907-914,
https://doi.org/10.2298/ABS1004907R . .

TY  - JOUR
AU  - Beletić, Anđelo
AU  - Đorđević, Valentina
AU  - Dudvarski-Ilić, Aleksandra
AU  - Obradović, Ivana
AU  - Mirković, Duško
AU  - Ilić, Mirka
AU  - Radojković, Dragica
AU  - Majkić-Singh, Nada
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1163
AB  - Alpha-1-antitrypsin deficiency is a potentially lethal genetic disorder, which has pulmonary and liver manifestations. The standardized biochemical and molecular diagnostic protocol for detection of clinically relevant alleles is needed. The paper summarizes current concepts about AATD, describes the potentials of isoelectric focusing and PCR amplification-reverse allele specific oligonucleotide hybridization assay in the detection of affected individuals and shortly presents our experiences in the evaluation of AATD. We conclude that the systematic clinical laboratory approach to AATD might be based on the combination of mentioned methods, coordinated by alpha-1-antritrypsin quantification. Additionally, its complete medical implementation is achieved through teamwork between clinical chemists, molecular biologists and clinicians.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Isoelectrofocusing and PCR Amplification-Reverse Hybridization Assay in Evaluation of Alpha-1-Antitrypsin Deficiency
VL  - 28
IS  - 4
SP  - 241
EP  - 247
DO  - 10.2478/v10011-009-0023-x
ER  - 

@article{
author = "Beletić, Anđelo and Đorđević, Valentina and Dudvarski-Ilić, Aleksandra and Obradović, Ivana and Mirković, Duško and Ilić, Mirka and Radojković, Dragica and Majkić-Singh, Nada",
year = "2009",
abstract = "Alpha-1-antitrypsin deficiency is a potentially lethal genetic disorder, which has pulmonary and liver manifestations. The standardized biochemical and molecular diagnostic protocol for detection of clinically relevant alleles is needed. The paper summarizes current concepts about AATD, describes the potentials of isoelectric focusing and PCR amplification-reverse allele specific oligonucleotide hybridization assay in the detection of affected individuals and shortly presents our experiences in the evaluation of AATD. We conclude that the systematic clinical laboratory approach to AATD might be based on the combination of mentioned methods, coordinated by alpha-1-antritrypsin quantification. Additionally, its complete medical implementation is achieved through teamwork between clinical chemists, molecular biologists and clinicians.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Isoelectrofocusing and PCR Amplification-Reverse Hybridization Assay in Evaluation of Alpha-1-Antitrypsin Deficiency",
volume = "28",
number = "4",
pages = "241-247",
doi = "10.2478/v10011-009-0023-x"
}

Beletić, A., Đorđević, V., Dudvarski-Ilić, A., Obradović, I., Mirković, D., Ilić, M., Radojković, D.,& Majkić-Singh, N.. (2009). Isoelectrofocusing and PCR Amplification-Reverse Hybridization Assay in Evaluation of Alpha-1-Antitrypsin Deficiency. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 28(4), 241-247.
https://doi.org/10.2478/v10011-009-0023-x

Beletić A, Đorđević V, Dudvarski-Ilić A, Obradović I, Mirković D, Ilić M, Radojković D, Majkić-Singh N. Isoelectrofocusing and PCR Amplification-Reverse Hybridization Assay in Evaluation of Alpha-1-Antitrypsin Deficiency. in Journal of Medical Biochemistry. 2009;28(4):241-247.
doi:10.2478/v10011-009-0023-x .

Beletić, Anđelo, Đorđević, Valentina, Dudvarski-Ilić, Aleksandra, Obradović, Ivana, Mirković, Duško, Ilić, Mirka, Radojković, Dragica, Majkić-Singh, Nada, "Isoelectrofocusing and PCR Amplification-Reverse Hybridization Assay in Evaluation of Alpha-1-Antitrypsin Deficiency" in Journal of Medical Biochemistry, 28, no. 4 (2009):241-247,
https://doi.org/10.2478/v10011-009-0023-x . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Majkić-Singh, Nada
AU  - Mirković, Duško
AU  - Beletić, Anđelo
AU  - Pejanović, Svetlana D.
AU  - Vujanić, Svetlana T.
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1189
AB  - Deficient serum 25-hydroxyvitamin D [25(OH)D] may contribute to the impaired bone turnover of end stage renal disease patients. In 112 hemodialysed patients we analysed the relation between 25(OH)D and bone alkaline phosphatase (BALP), beta-CrossLaps (beta-CTx) and iPTH. We analysed parameters according to the manufacturers' instructions. We found potentially significant vitamin D deficiency: 71% of patients had 25(OH)D levels below 50 nmol/L. In patients with iPTH below 150 pg/mL (n = 57), we observed significantly low 25(OH) (p lt 0.01). In addition, patients with iPTH above 300 pg/mL had higher BALP levels (p lt 0.05). There were negative correlations between serum 25(OH)D and both BALP and iPTH (r = -0.225, p lt 0.05 and r = -0.331, p lt 0.05). Beta-CTx levels were significantly higher in patients who did not receive vitamin D supplementation (p lt 0.01). In addition, reduced BALP and iPTH levels indicate decreased bone turnover. Recorded data could signify that vitamin D deficiency may contribute to the impaired bone metabolism of hemodialysis patients. (Clin. Lab. 2009;55:333-339)
PB  - Clin Lab Publ, Heidelberg
T2  - Clinical Laboratory
T1  - Relation Between 25(OH)-Vitamin D Deficiency and Markers of Bone Formation and Resorption in Haemodialysis Patients
VL  - 55
IS  - 9-10
SP  - 333
EP  - 339
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1189
ER  - 

@article{
author = "Milinković, Neda and Majkić-Singh, Nada and Mirković, Duško and Beletić, Anđelo and Pejanović, Svetlana D. and Vujanić, Svetlana T.",
year = "2009",
abstract = "Deficient serum 25-hydroxyvitamin D [25(OH)D] may contribute to the impaired bone turnover of end stage renal disease patients. In 112 hemodialysed patients we analysed the relation between 25(OH)D and bone alkaline phosphatase (BALP), beta-CrossLaps (beta-CTx) and iPTH. We analysed parameters according to the manufacturers' instructions. We found potentially significant vitamin D deficiency: 71% of patients had 25(OH)D levels below 50 nmol/L. In patients with iPTH below 150 pg/mL (n = 57), we observed significantly low 25(OH) (p lt 0.01). In addition, patients with iPTH above 300 pg/mL had higher BALP levels (p lt 0.05). There were negative correlations between serum 25(OH)D and both BALP and iPTH (r = -0.225, p lt 0.05 and r = -0.331, p lt 0.05). Beta-CTx levels were significantly higher in patients who did not receive vitamin D supplementation (p lt 0.01). In addition, reduced BALP and iPTH levels indicate decreased bone turnover. Recorded data could signify that vitamin D deficiency may contribute to the impaired bone metabolism of hemodialysis patients. (Clin. Lab. 2009;55:333-339)",
publisher = "Clin Lab Publ, Heidelberg",
journal = "Clinical Laboratory",
title = "Relation Between 25(OH)-Vitamin D Deficiency and Markers of Bone Formation and Resorption in Haemodialysis Patients",
volume = "55",
number = "9-10",
pages = "333-339",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1189"
}

Milinković, N., Majkić-Singh, N., Mirković, D., Beletić, A., Pejanović, S. D.,& Vujanić, S. T.. (2009). Relation Between 25(OH)-Vitamin D Deficiency and Markers of Bone Formation and Resorption in Haemodialysis Patients. in Clinical Laboratory
Clin Lab Publ, Heidelberg., 55(9-10), 333-339.
https://hdl.handle.net/21.15107/rcub_farfar_1189

Milinković N, Majkić-Singh N, Mirković D, Beletić A, Pejanović SD, Vujanić ST. Relation Between 25(OH)-Vitamin D Deficiency and Markers of Bone Formation and Resorption in Haemodialysis Patients. in Clinical Laboratory. 2009;55(9-10):333-339.
https://hdl.handle.net/21.15107/rcub_farfar_1189 .

Milinković, Neda, Majkić-Singh, Nada, Mirković, Duško, Beletić, Anđelo, Pejanović, Svetlana D., Vujanić, Svetlana T., "Relation Between 25(OH)-Vitamin D Deficiency and Markers of Bone Formation and Resorption in Haemodialysis Patients" in Clinical Laboratory, 55, no. 9-10 (2009):333-339,
https://hdl.handle.net/21.15107/rcub_farfar_1189 .