TY  - JOUR
AU  - Stajić, Ana
AU  - Anđelković, Marija
AU  - Dikić, Nenad
AU  - Rasić, J.
AU  - Vukašinović-Vesić, Milica
AU  - Ivanović, D.
AU  - Jančić-Stojanović, Biljana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2869
AB  - From 1st January 2017 higenamine was added on the WADA (World Anti-doping Agency) Prohibited list under S3 group beta-2 agonists as at all times banned substance for the athletes. The main origine of higenamine (or norcoclaurine) are different plants including Nandina domestica, Aconitum carmichaelii, Asarum heterotropioides, Galium divaricatum, Annona squamosa, Nelumbo nucifera etc. Higenamine main use is related to weight loss and it could be found (un)labeled in different dietary supplements. The objective of this study was development of sensitive and reliable UHPLC/MS/MS method for determination of higenamine in various dietary supplement samples. In order to obtain high method sensitivity, hydrophilic interaction liquid chromatography (HILIC) mode was applied. Separation was carried out on UHPLC Acquity BEH HILIC analytical column (2.1 mm x 100 mm, 1.7 mu m particle size). Mobile phase consisted of 0.1% formic acid in water and acetonitrile, respectively, was mixed in ratio of 30:70, v/v. Flow rate was set at 0.2 mL min(-1). Quercetin was used as an internal standard. ESI (+) source ionization mode using multi reaction monitoring (MRM) mode was utilized and three ion transitions of higenamine were followed 272.08 -> 107.01, 272.08 -> 161.07 and 272.08 -> 77.08. Developed method was fully validated and applied for identification and quantification of higenamine in different dietary supplements. According to the results, the most of investigated supplements were free of higenamine, and on the other hand, presence of higenamine was confirmed in some samples while it was not declared on the label.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Determination of higenamine in dietary supplements by UHPLC/MS/MS method
VL  - 146
SP  - 48
EP  - 52
DO  - 10.1016/j.jpba.2017.08.017
ER  - 

@article{
author = "Stajić, Ana and Anđelković, Marija and Dikić, Nenad and Rasić, J. and Vukašinović-Vesić, Milica and Ivanović, D. and Jančić-Stojanović, Biljana",
year = "2017",
abstract = "From 1st January 2017 higenamine was added on the WADA (World Anti-doping Agency) Prohibited list under S3 group beta-2 agonists as at all times banned substance for the athletes. The main origine of higenamine (or norcoclaurine) are different plants including Nandina domestica, Aconitum carmichaelii, Asarum heterotropioides, Galium divaricatum, Annona squamosa, Nelumbo nucifera etc. Higenamine main use is related to weight loss and it could be found (un)labeled in different dietary supplements. The objective of this study was development of sensitive and reliable UHPLC/MS/MS method for determination of higenamine in various dietary supplement samples. In order to obtain high method sensitivity, hydrophilic interaction liquid chromatography (HILIC) mode was applied. Separation was carried out on UHPLC Acquity BEH HILIC analytical column (2.1 mm x 100 mm, 1.7 mu m particle size). Mobile phase consisted of 0.1% formic acid in water and acetonitrile, respectively, was mixed in ratio of 30:70, v/v. Flow rate was set at 0.2 mL min(-1). Quercetin was used as an internal standard. ESI (+) source ionization mode using multi reaction monitoring (MRM) mode was utilized and three ion transitions of higenamine were followed 272.08 -> 107.01, 272.08 -> 161.07 and 272.08 -> 77.08. Developed method was fully validated and applied for identification and quantification of higenamine in different dietary supplements. According to the results, the most of investigated supplements were free of higenamine, and on the other hand, presence of higenamine was confirmed in some samples while it was not declared on the label.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Determination of higenamine in dietary supplements by UHPLC/MS/MS method",
volume = "146",
pages = "48-52",
doi = "10.1016/j.jpba.2017.08.017"
}

Stajić, A., Anđelković, M., Dikić, N., Rasić, J., Vukašinović-Vesić, M., Ivanović, D.,& Jančić-Stojanović, B.. (2017). Determination of higenamine in dietary supplements by UHPLC/MS/MS method. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science BV, Amsterdam., 146, 48-52.
https://doi.org/10.1016/j.jpba.2017.08.017

Stajić A, Anđelković M, Dikić N, Rasić J, Vukašinović-Vesić M, Ivanović D, Jančić-Stojanović B. Determination of higenamine in dietary supplements by UHPLC/MS/MS method. in Journal of Pharmaceutical and Biomedical Analysis. 2017;146:48-52.
doi:10.1016/j.jpba.2017.08.017 .

Stajić, Ana, Anđelković, Marija, Dikić, Nenad, Rasić, J., Vukašinović-Vesić, Milica, Ivanović, D., Jančić-Stojanović, Biljana, "Determination of higenamine in dietary supplements by UHPLC/MS/MS method" in Journal of Pharmaceutical and Biomedical Analysis, 146 (2017):48-52,
https://doi.org/10.1016/j.jpba.2017.08.017 . .

TY  - JOUR
AU  - Malenović, Anđelija
AU  - Vemić, Ana
AU  - Kostić, Nada
AU  - Ivanović, D.
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1916
AB  - In this paper, a chemometrically assisted validation of RP-HPLC method, intended for the quantitative analysis of cefuroxime axetil (A and B), cefuroxime acid, cefuroxime lactone, cefuroxime axetil sulfoxide (A and B), a dagger(3)-cefuroxime axetil and anti cefuroxime axetil (A and B) in tablets, is presented. Since the successful separation could be achieved with the mobile phase containing only methanol and water, Luna C18 column was selected for the analysis. Under these circumstances, the optimization was quite straightforward and included only a fine tuning of the chromatographic conditions to reduce total run time and maintain the achieved separation. The established method was then subjected to the method validation and the required validation parameters were tested. For the robustness evaluation, a fractional factorial 2(4-1) design was utilized and factors that might significantly affect the system performance were defined. For the significant factors, the non-significant intervals were determined and the acceptable system suitability limit for resolution factor between cefuroxime axetil A and cefuroxime axetil a dagger(3) isomer (R (2)) was calculated. As the other validation parameters were also found to be suitable, the possibility to apply the proposed method for the determination of cefuroxime axetil, cefuroxime acid, cefuroxime lactone, cefuroxime axetil sulfoxide, a dagger(3)-cefuroxime axetil and anti cefuroxime axetil in any laboratory under different circumstances is proven.
PB  - Springer Heidelberg, Heidelberg
T2  - Chromatographia
T1  - Evaluation of RP-HPLC Method Intended for the Analysis of Cefuroxime Axetil and ITS Impurities Supported by Experimental Design
VL  - 76
IS  - 5-6
SP  - 293
EP  - 298
DO  - 10.1007/s10337-013-2391-0
ER  - 

@article{
author = "Malenović, Anđelija and Vemić, Ana and Kostić, Nada and Ivanović, D.",
year = "2013",
abstract = "In this paper, a chemometrically assisted validation of RP-HPLC method, intended for the quantitative analysis of cefuroxime axetil (A and B), cefuroxime acid, cefuroxime lactone, cefuroxime axetil sulfoxide (A and B), a dagger(3)-cefuroxime axetil and anti cefuroxime axetil (A and B) in tablets, is presented. Since the successful separation could be achieved with the mobile phase containing only methanol and water, Luna C18 column was selected for the analysis. Under these circumstances, the optimization was quite straightforward and included only a fine tuning of the chromatographic conditions to reduce total run time and maintain the achieved separation. The established method was then subjected to the method validation and the required validation parameters were tested. For the robustness evaluation, a fractional factorial 2(4-1) design was utilized and factors that might significantly affect the system performance were defined. For the significant factors, the non-significant intervals were determined and the acceptable system suitability limit for resolution factor between cefuroxime axetil A and cefuroxime axetil a dagger(3) isomer (R (2)) was calculated. As the other validation parameters were also found to be suitable, the possibility to apply the proposed method for the determination of cefuroxime axetil, cefuroxime acid, cefuroxime lactone, cefuroxime axetil sulfoxide, a dagger(3)-cefuroxime axetil and anti cefuroxime axetil in any laboratory under different circumstances is proven.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Chromatographia",
title = "Evaluation of RP-HPLC Method Intended for the Analysis of Cefuroxime Axetil and ITS Impurities Supported by Experimental Design",
volume = "76",
number = "5-6",
pages = "293-298",
doi = "10.1007/s10337-013-2391-0"
}

Malenović, A., Vemić, A., Kostić, N.,& Ivanović, D.. (2013). Evaluation of RP-HPLC Method Intended for the Analysis of Cefuroxime Axetil and ITS Impurities Supported by Experimental Design. in Chromatographia
Springer Heidelberg, Heidelberg., 76(5-6), 293-298.
https://doi.org/10.1007/s10337-013-2391-0

Malenović A, Vemić A, Kostić N, Ivanović D. Evaluation of RP-HPLC Method Intended for the Analysis of Cefuroxime Axetil and ITS Impurities Supported by Experimental Design. in Chromatographia. 2013;76(5-6):293-298.
doi:10.1007/s10337-013-2391-0 .

Malenović, Anđelija, Vemić, Ana, Kostić, Nada, Ivanović, D., "Evaluation of RP-HPLC Method Intended for the Analysis of Cefuroxime Axetil and ITS Impurities Supported by Experimental Design" in Chromatographia, 76, no. 5-6 (2013):293-298,
https://doi.org/10.1007/s10337-013-2391-0 . .

TY  - CHAP
AU  - Jančić-Stojanović, Biljana
AU  - Malenović, Anđelija
AU  - Ivanović, D
AU  - Medenica, Mirjana
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1614
AB  - Artificial neural networks (ANNs) present a powerful tool for the modeling of chromatographic retention and resolution as they provide the ability to predict the effects of changes in chosen chromatographic parameters under various conditions. The tracking of certain chromatographic parameters presents the most important part in the optimization of any chromatographic method. One common situation is that in which opposing objectives (e.g., minimization of run time and maximization of chromatographic resolution) must be achieved. For this reason, different kinds of eluents are used in liquid chromatography (LC) methods, offering different mechanisms of retention and separation. The choice of eluent(s) mainly depends on the chemical structures and physical and chemical properties of the analytes. In addition, depending on the researchers' experience and knowledge, different optimization costs are acceptable. In this chapter, the feasibility of ANNs for modeling a chromatographic system was evaluated using the example of a microemulsion LC (MELC) separation of perindopril tert-butylamine and its structurally similar impurities (perindoprilat, Y31, Y32 and Y33). The selected complex mixture and finally defined microemulsion eluent composition demonstrated the application of ANNs in LC method optimization in a most descriptive and comprehensive way. In accordance with the situation and previous experience, a multiple-layer perceptron (MLP) was selected; MLP is the ANN form most commonly used for retention modeling. For the evaluation of the ANNs, a set of thirty experiments defined by central composite design (CCD) was performed. The influence of five factors on the chromatographic system was examined. ANNs were used in anticipation of the retention behavior of the eluting substances as well as of the chromatographic resolution (nine outputs in total). To obtain the networks with best characteristics, two separate networks (the first for retention factors and the second for resolution factors) were constructed from the experimental results. In this way, the number of nodes in the input and output layers were defined so that the network topologies for retention and resolution factors were 5-x-5 and 5-x-4, respectively. By employing D-optimal design in the first part of network optimization, the number of nodes in the hidden layer and the number of experimental data points used for training were simultaneously investigated. Furthermore, a series of training algorithms was applied to the current MLP network. The Backpropagation (BP), Conjugate Gradient-descent (CG), Quick Propagation (QP), Quasi-Newton (QN), and Delta-bar-Delta (DBD) algorithms were used to obtain the optimal network. The predictive ability of the optimized neural network was evaluated using several statistical tests. Consequently, the successful application of ANNs in the optimization of an LC method in a pharmaceutical analysis was demonstrated via the body of analyzed and discussed data.
PB  - Nova Science Publishers, Inc.
T2  - Artificial Neural Networks
T1  - Artificial neural networks in the optimization of microemulsion liquid chromatography retention and resolution
SP  - 387
EP  - 410
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1614
ER  - 

@inbook{
author = "Jančić-Stojanović, Biljana and Malenović, Anđelija and Ivanović, D and Medenica, Mirjana",
year = "2011",
abstract = "Artificial neural networks (ANNs) present a powerful tool for the modeling of chromatographic retention and resolution as they provide the ability to predict the effects of changes in chosen chromatographic parameters under various conditions. The tracking of certain chromatographic parameters presents the most important part in the optimization of any chromatographic method. One common situation is that in which opposing objectives (e.g., minimization of run time and maximization of chromatographic resolution) must be achieved. For this reason, different kinds of eluents are used in liquid chromatography (LC) methods, offering different mechanisms of retention and separation. The choice of eluent(s) mainly depends on the chemical structures and physical and chemical properties of the analytes. In addition, depending on the researchers' experience and knowledge, different optimization costs are acceptable. In this chapter, the feasibility of ANNs for modeling a chromatographic system was evaluated using the example of a microemulsion LC (MELC) separation of perindopril tert-butylamine and its structurally similar impurities (perindoprilat, Y31, Y32 and Y33). The selected complex mixture and finally defined microemulsion eluent composition demonstrated the application of ANNs in LC method optimization in a most descriptive and comprehensive way. In accordance with the situation and previous experience, a multiple-layer perceptron (MLP) was selected; MLP is the ANN form most commonly used for retention modeling. For the evaluation of the ANNs, a set of thirty experiments defined by central composite design (CCD) was performed. The influence of five factors on the chromatographic system was examined. ANNs were used in anticipation of the retention behavior of the eluting substances as well as of the chromatographic resolution (nine outputs in total). To obtain the networks with best characteristics, two separate networks (the first for retention factors and the second for resolution factors) were constructed from the experimental results. In this way, the number of nodes in the input and output layers were defined so that the network topologies for retention and resolution factors were 5-x-5 and 5-x-4, respectively. By employing D-optimal design in the first part of network optimization, the number of nodes in the hidden layer and the number of experimental data points used for training were simultaneously investigated. Furthermore, a series of training algorithms was applied to the current MLP network. The Backpropagation (BP), Conjugate Gradient-descent (CG), Quick Propagation (QP), Quasi-Newton (QN), and Delta-bar-Delta (DBD) algorithms were used to obtain the optimal network. The predictive ability of the optimized neural network was evaluated using several statistical tests. Consequently, the successful application of ANNs in the optimization of an LC method in a pharmaceutical analysis was demonstrated via the body of analyzed and discussed data.",
publisher = "Nova Science Publishers, Inc.",
journal = "Artificial Neural Networks",
booktitle = "Artificial neural networks in the optimization of microemulsion liquid chromatography retention and resolution",
pages = "387-410",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1614"
}

Jančić-Stojanović, B., Malenović, A., Ivanović, D.,& Medenica, M.. (2011). Artificial neural networks in the optimization of microemulsion liquid chromatography retention and resolution. in Artificial Neural Networks
Nova Science Publishers, Inc.., 387-410.
https://hdl.handle.net/21.15107/rcub_farfar_1614

Jančić-Stojanović B, Malenović A, Ivanović D, Medenica M. Artificial neural networks in the optimization of microemulsion liquid chromatography retention and resolution. in Artificial Neural Networks. 2011;:387-410.
https://hdl.handle.net/21.15107/rcub_farfar_1614 .

Jančić-Stojanović, Biljana, Malenović, Anđelija, Ivanović, D, Medenica, Mirjana, "Artificial neural networks in the optimization of microemulsion liquid chromatography retention and resolution" in Artificial Neural Networks (2011):387-410,
https://hdl.handle.net/21.15107/rcub_farfar_1614 .

TY  - JOUR
AU  - Jančić-Stojanović, Biljana
AU  - Rakić, Tijana
AU  - Kostić, Nada
AU  - Vemić, Ana
AU  - Malenović, Anđelija
AU  - Ivanović, D.
AU  - Medenica, Mirjana
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1477
AB  - In this paper a new chromatographic response function (CRF) is designed and proposed for utilization in the optimization strategies. The function capability to represent the overall quality of a experimentally obtained chromatograms was compared to the other two objective functions and proved to give more accurate and reliable results. The new CRF has improved concept of separation and time term estimation. It reflects all important defects of the chromatogram such as the appearance of asymmetrical or overlapping peaks and prolonged elution time and allows the appropriate weighting of each of them. The LC separation of raloxifene and its four impurities was evaluated through the central composite design experimental plan choosing the new CRF to be the only output of the system. The function demonstrated the ability to judge the impact of the complex interactions of the selected chromatographic parameters (acetonitrile content in the mobile phase, sodium dodecyl sulfate concentration in the water phase, pH of the mobile phase and column temperature) on the mixture behavior and led to the determination of the optimal separation conditions. The newly developed CRF proved to have the advanced performances and it presents the important step forward in the optimization of the chromatographic separation.
PB  - Elsevier Science BV, Amsterdam
T2  - Talanta
T1  - Advancement in optimization tactic achieved by newly developed chromatographic response function: Application to LC separation of raloxifene and its impurities
VL  - 85
IS  - 3
SP  - 1453
EP  - 1460
DO  - 10.1016/j.talanta.2011.06.029
ER  - 

@article{
author = "Jančić-Stojanović, Biljana and Rakić, Tijana and Kostić, Nada and Vemić, Ana and Malenović, Anđelija and Ivanović, D. and Medenica, Mirjana",
year = "2011",
abstract = "In this paper a new chromatographic response function (CRF) is designed and proposed for utilization in the optimization strategies. The function capability to represent the overall quality of a experimentally obtained chromatograms was compared to the other two objective functions and proved to give more accurate and reliable results. The new CRF has improved concept of separation and time term estimation. It reflects all important defects of the chromatogram such as the appearance of asymmetrical or overlapping peaks and prolonged elution time and allows the appropriate weighting of each of them. The LC separation of raloxifene and its four impurities was evaluated through the central composite design experimental plan choosing the new CRF to be the only output of the system. The function demonstrated the ability to judge the impact of the complex interactions of the selected chromatographic parameters (acetonitrile content in the mobile phase, sodium dodecyl sulfate concentration in the water phase, pH of the mobile phase and column temperature) on the mixture behavior and led to the determination of the optimal separation conditions. The newly developed CRF proved to have the advanced performances and it presents the important step forward in the optimization of the chromatographic separation.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Talanta",
title = "Advancement in optimization tactic achieved by newly developed chromatographic response function: Application to LC separation of raloxifene and its impurities",
volume = "85",
number = "3",
pages = "1453-1460",
doi = "10.1016/j.talanta.2011.06.029"
}

Jančić-Stojanović, B., Rakić, T., Kostić, N., Vemić, A., Malenović, A., Ivanović, D.,& Medenica, M.. (2011). Advancement in optimization tactic achieved by newly developed chromatographic response function: Application to LC separation of raloxifene and its impurities. in Talanta
Elsevier Science BV, Amsterdam., 85(3), 1453-1460.
https://doi.org/10.1016/j.talanta.2011.06.029

Jančić-Stojanović B, Rakić T, Kostić N, Vemić A, Malenović A, Ivanović D, Medenica M. Advancement in optimization tactic achieved by newly developed chromatographic response function: Application to LC separation of raloxifene and its impurities. in Talanta. 2011;85(3):1453-1460.
doi:10.1016/j.talanta.2011.06.029 .

Jančić-Stojanović, Biljana, Rakić, Tijana, Kostić, Nada, Vemić, Ana, Malenović, Anđelija, Ivanović, D., Medenica, Mirjana, "Advancement in optimization tactic achieved by newly developed chromatographic response function: Application to LC separation of raloxifene and its impurities" in Talanta, 85, no. 3 (2011):1453-1460,
https://doi.org/10.1016/j.talanta.2011.06.029 . .

TY  - JOUR
AU  - Masković, M.
AU  - Jančić-Stojanović, Biljana
AU  - Malenović, Anđelija
AU  - Ivanović, D.
AU  - Medenica, Mirjana
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1399
AB  - Robustness testing is an important part of method validation. Many ideas on robustness testing can be found in the literature, but the most useful approach is application of experimental design. In the work discussed in this paper, Plackett-Burman design was used for robustness testing of an LC method. Robustness was assessed for a previously validated method developed for chromatographic analysis of perindopril tert-butylamine and its impurities. Eleven factors (seven real and four dummies) in twelve experiments were examined. Robustness was assessed by graphical (half-normal probability plots and Pareto charts) and statistical (t-test) methods. For estimation of the standard error of effect (necessary for t-test estimation) calculations based on negligible effects and Dong's algorithm were used at the significance levels alpha = 0.05 and alpha = 0.01. In this way the effect of the factors was completely defined and, furthermore, nonsignificant intervals for significant variables were calculated. Finally, on the basis of the worst-case situation, system-suitability tests were performed and acceptance limits for certain values were calculated.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Chromatographica
T1  - Assessment of Liquid Chromatographic Method Robustness by Use of Plackett-Burman Design
VL  - 22
IS  - 2
SP  - 281
EP  - 296
DO  - 10.1556/AChrom.22.2010.2.10
ER  - 

@article{
author = "Masković, M. and Jančić-Stojanović, Biljana and Malenović, Anđelija and Ivanović, D. and Medenica, Mirjana",
year = "2010",
abstract = "Robustness testing is an important part of method validation. Many ideas on robustness testing can be found in the literature, but the most useful approach is application of experimental design. In the work discussed in this paper, Plackett-Burman design was used for robustness testing of an LC method. Robustness was assessed for a previously validated method developed for chromatographic analysis of perindopril tert-butylamine and its impurities. Eleven factors (seven real and four dummies) in twelve experiments were examined. Robustness was assessed by graphical (half-normal probability plots and Pareto charts) and statistical (t-test) methods. For estimation of the standard error of effect (necessary for t-test estimation) calculations based on negligible effects and Dong's algorithm were used at the significance levels alpha = 0.05 and alpha = 0.01. In this way the effect of the factors was completely defined and, furthermore, nonsignificant intervals for significant variables were calculated. Finally, on the basis of the worst-case situation, system-suitability tests were performed and acceptance limits for certain values were calculated.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Chromatographica",
title = "Assessment of Liquid Chromatographic Method Robustness by Use of Plackett-Burman Design",
volume = "22",
number = "2",
pages = "281-296",
doi = "10.1556/AChrom.22.2010.2.10"
}

Masković, M., Jančić-Stojanović, B., Malenović, A., Ivanović, D.,& Medenica, M.. (2010). Assessment of Liquid Chromatographic Method Robustness by Use of Plackett-Burman Design. in Acta Chromatographica
Akademiai Kiado Zrt, Budapest., 22(2), 281-296.
https://doi.org/10.1556/AChrom.22.2010.2.10

Masković M, Jančić-Stojanović B, Malenović A, Ivanović D, Medenica M. Assessment of Liquid Chromatographic Method Robustness by Use of Plackett-Burman Design. in Acta Chromatographica. 2010;22(2):281-296.
doi:10.1556/AChrom.22.2010.2.10 .

Masković, M., Jančić-Stojanović, Biljana, Malenović, Anđelija, Ivanović, D., Medenica, Mirjana, "Assessment of Liquid Chromatographic Method Robustness by Use of Plackett-Burman Design" in Acta Chromatographica, 22, no. 2 (2010):281-296,
https://doi.org/10.1556/AChrom.22.2010.2.10 . .

TY  - JOUR
AU  - Jančić-Stojanović, Biljana
AU  - Malenović, Anđelija
AU  - Ivanović, D.
AU  - Rakić, Tijana
AU  - Medenica, Mirjana
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1250
AB  - The aim of this study was the chemometrical evaluation of ropinirole and its impurity's (4-[2(dipropylamino)ethyl]-1H-indol-2,3-dione) chromatographic behavior in systematic and the most efficient way. For that purpose, as very descriptive, response surface designs are most preferable. Face-centered central composite design (CCD) with 2(3) full factorial design, +/- 1 star design and four replication in central point was applied for a response surface study, in order to examine in depth the effects of the most important factors. Factors-independent variables (acetonitrile content, pH of the mobile phase and concentration of sodium heptane sulfonate in water phase) were extracted from the preliminary study and as dependent variables five responses (retention factor of ropinirole, retentin factor of its impurity, resolution, symmetry of ropinirole peak and symmetry of impurity peak) were selected. For the improvement of method development and optimization step, Derringer's desirability function was applied to simultaneously optimize the five chosen responses. The procedure allowed deduction of optimal conditions and the predicted optimum was acetonitrile-5 mM of sodium heptane sulfonate (21.6:78.4, v/v), pH of the mobile phase adjusted at 2.0 with ortho phosphoric acid. By calculating global desirability's determination coefficients (R-D(2)), as well as by the visual inspection of 3D graphs for global desirability, robustness of the proposed method was also estimated.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Chromatography A
T1  - Chemometrical evaluation of ropinirole and its impurity's chromatographic behavior
VL  - 1216
IS  - 8
SP  - 1263
EP  - 1269
DO  - 10.1016/j.chroma.2008.10.059
ER  - 

@article{
author = "Jančić-Stojanović, Biljana and Malenović, Anđelija and Ivanović, D. and Rakić, Tijana and Medenica, Mirjana",
year = "2009",
abstract = "The aim of this study was the chemometrical evaluation of ropinirole and its impurity's (4-[2(dipropylamino)ethyl]-1H-indol-2,3-dione) chromatographic behavior in systematic and the most efficient way. For that purpose, as very descriptive, response surface designs are most preferable. Face-centered central composite design (CCD) with 2(3) full factorial design, +/- 1 star design and four replication in central point was applied for a response surface study, in order to examine in depth the effects of the most important factors. Factors-independent variables (acetonitrile content, pH of the mobile phase and concentration of sodium heptane sulfonate in water phase) were extracted from the preliminary study and as dependent variables five responses (retention factor of ropinirole, retentin factor of its impurity, resolution, symmetry of ropinirole peak and symmetry of impurity peak) were selected. For the improvement of method development and optimization step, Derringer's desirability function was applied to simultaneously optimize the five chosen responses. The procedure allowed deduction of optimal conditions and the predicted optimum was acetonitrile-5 mM of sodium heptane sulfonate (21.6:78.4, v/v), pH of the mobile phase adjusted at 2.0 with ortho phosphoric acid. By calculating global desirability's determination coefficients (R-D(2)), as well as by the visual inspection of 3D graphs for global desirability, robustness of the proposed method was also estimated.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Chromatography A",
title = "Chemometrical evaluation of ropinirole and its impurity's chromatographic behavior",
volume = "1216",
number = "8",
pages = "1263-1269",
doi = "10.1016/j.chroma.2008.10.059"
}

Jančić-Stojanović, B., Malenović, A., Ivanović, D., Rakić, T.,& Medenica, M.. (2009). Chemometrical evaluation of ropinirole and its impurity's chromatographic behavior. in Journal of Chromatography A
Elsevier Science BV, Amsterdam., 1216(8), 1263-1269.
https://doi.org/10.1016/j.chroma.2008.10.059

Jančić-Stojanović B, Malenović A, Ivanović D, Rakić T, Medenica M. Chemometrical evaluation of ropinirole and its impurity's chromatographic behavior. in Journal of Chromatography A. 2009;1216(8):1263-1269.
doi:10.1016/j.chroma.2008.10.059 .

Jančić-Stojanović, Biljana, Malenović, Anđelija, Ivanović, D., Rakić, Tijana, Medenica, Mirjana, "Chemometrical evaluation of ropinirole and its impurity's chromatographic behavior" in Journal of Chromatography A, 1216, no. 8 (2009):1263-1269,
https://doi.org/10.1016/j.chroma.2008.10.059 . .

TY  - JOUR
AU  - Jančić-Stojanović, Biljana
AU  - Ivanović, D.
AU  - Malenović, Anđelija
AU  - Medenica, Mirjana
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1247
AB  - Artificial neural networks (ANN) are biologically inspired computer programs designed to simulate the way in which the human brain processes the information. In the past few years, coupling of experimental design (ED) and ANN became useful tool in the method optimization. This paper presents the application of ED-ANN in analysis of chromatographic behavior of indinavir and its degradation products. According to preliminary study, full factorial design 2(4) was chosen to set input variables for network training. Experimental data (inputs) and results for retention factors from experiments (outputs) were used to train the ANN with aim to define correlation among variables. For networks training multi-layer perceptron (MLP) with back propagation (BP) algorithm was used. Network with the lowest root mean square (RMS) had 4-8-3 topology. Predicted data were in good agreement with experimental data (correlation was higher than 0.9713 for training set). Regression statistics confirmed good ability of trained network to predict compounds retention.
PB  - Elsevier Science BV, Amsterdam
T2  - Talanta
T1  - Artificial neural networks in analysis of indinavir and its degradation products retention
VL  - 78
IS  - 1
SP  - 107
EP  - 112
DO  - 10.1016/j.talanta.2008.10.066
ER  - 

@article{
author = "Jančić-Stojanović, Biljana and Ivanović, D. and Malenović, Anđelija and Medenica, Mirjana",
year = "2009",
abstract = "Artificial neural networks (ANN) are biologically inspired computer programs designed to simulate the way in which the human brain processes the information. In the past few years, coupling of experimental design (ED) and ANN became useful tool in the method optimization. This paper presents the application of ED-ANN in analysis of chromatographic behavior of indinavir and its degradation products. According to preliminary study, full factorial design 2(4) was chosen to set input variables for network training. Experimental data (inputs) and results for retention factors from experiments (outputs) were used to train the ANN with aim to define correlation among variables. For networks training multi-layer perceptron (MLP) with back propagation (BP) algorithm was used. Network with the lowest root mean square (RMS) had 4-8-3 topology. Predicted data were in good agreement with experimental data (correlation was higher than 0.9713 for training set). Regression statistics confirmed good ability of trained network to predict compounds retention.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Talanta",
title = "Artificial neural networks in analysis of indinavir and its degradation products retention",
volume = "78",
number = "1",
pages = "107-112",
doi = "10.1016/j.talanta.2008.10.066"
}

Jančić-Stojanović, B., Ivanović, D., Malenović, A.,& Medenica, M.. (2009). Artificial neural networks in analysis of indinavir and its degradation products retention. in Talanta
Elsevier Science BV, Amsterdam., 78(1), 107-112.
https://doi.org/10.1016/j.talanta.2008.10.066

Jančić-Stojanović B, Ivanović D, Malenović A, Medenica M. Artificial neural networks in analysis of indinavir and its degradation products retention. in Talanta. 2009;78(1):107-112.
doi:10.1016/j.talanta.2008.10.066 .

Jančić-Stojanović, Biljana, Ivanović, D., Malenović, Anđelija, Medenica, Mirjana, "Artificial neural networks in analysis of indinavir and its degradation products retention" in Talanta, 78, no. 1 (2009):107-112,
https://doi.org/10.1016/j.talanta.2008.10.066 . .

TY  - JOUR
AU  - Jančić, Biljana
AU  - Medenica, Mirjana
AU  - Ivanović, D.
AU  - Janković, Saša
AU  - Malenović, Anđelija
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1068
AB  - In this paper, the mass spectrometry (MS) detection has been applied for screening of fosinopril sodium impurities which arise during forced stress study. Before MS analysis, liquid chromatographic method with suitable mobile phase composition was developed. The separation was done on SunFire 100 turn x 4.6 mm 3.5 mu m particle size column. The mobile phases which consisted of methanol-ammonium acetate buffer-acetic acid, in different ratios, were used in a preliminary study. Flow rate was 0.3 mL min(-1). Under these conditions, percent of methanol, concentration of ammonium acetate buffer and acetic acid content were tested simultaneously applying central composite design (CCD) and artificial neural network (ANN). The combinations of experimental design (ED) and ANN present powerful technique in method optimization. Input and output variables from CCD were used for network training, verification and testing. Multiple layer perceptron (MLP) with back propagation (BP) algorithm was chosen for network training. When the optimal neural topology was selected, network was trained by adjusting strength of connections between neurons in order to adapt the outputs of whole network to be closer to the desired outputs, or to minimize the sum of the squared errors. From the method optimization the following mobile phase composition was selected as appropriate: methanol-10 mM ammonium acetate buffer-acidic acid (80:19.5:0.5 v/v/v). This mobile phase was used as inlet for MS. According to molecular structure and literature data, electrospray positive ion mode was applied for analysis of fosinopril sodium and its impurities. The proposed method could be used for screening of fosinopril sodium impurities in bulk and pharmaceuticals, as well as for tracking the degradation under stress conditions.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Chromatography A
T1  - Monitoring of fosinopril sodium impurities by liquid chromatography-mass spectrometry including the neural networks in method evaluation
VL  - 1189
IS  - 1-2
SP  - 366
EP  - 373
DO  - 10.1016/j.chroma.2007.11.076
ER  - 

@article{
author = "Jančić, Biljana and Medenica, Mirjana and Ivanović, D. and Janković, Saša and Malenović, Anđelija",
year = "2008",
abstract = "In this paper, the mass spectrometry (MS) detection has been applied for screening of fosinopril sodium impurities which arise during forced stress study. Before MS analysis, liquid chromatographic method with suitable mobile phase composition was developed. The separation was done on SunFire 100 turn x 4.6 mm 3.5 mu m particle size column. The mobile phases which consisted of methanol-ammonium acetate buffer-acetic acid, in different ratios, were used in a preliminary study. Flow rate was 0.3 mL min(-1). Under these conditions, percent of methanol, concentration of ammonium acetate buffer and acetic acid content were tested simultaneously applying central composite design (CCD) and artificial neural network (ANN). The combinations of experimental design (ED) and ANN present powerful technique in method optimization. Input and output variables from CCD were used for network training, verification and testing. Multiple layer perceptron (MLP) with back propagation (BP) algorithm was chosen for network training. When the optimal neural topology was selected, network was trained by adjusting strength of connections between neurons in order to adapt the outputs of whole network to be closer to the desired outputs, or to minimize the sum of the squared errors. From the method optimization the following mobile phase composition was selected as appropriate: methanol-10 mM ammonium acetate buffer-acidic acid (80:19.5:0.5 v/v/v). This mobile phase was used as inlet for MS. According to molecular structure and literature data, electrospray positive ion mode was applied for analysis of fosinopril sodium and its impurities. The proposed method could be used for screening of fosinopril sodium impurities in bulk and pharmaceuticals, as well as for tracking the degradation under stress conditions.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Chromatography A",
title = "Monitoring of fosinopril sodium impurities by liquid chromatography-mass spectrometry including the neural networks in method evaluation",
volume = "1189",
number = "1-2",
pages = "366-373",
doi = "10.1016/j.chroma.2007.11.076"
}

Jančić, B., Medenica, M., Ivanović, D., Janković, S.,& Malenović, A.. (2008). Monitoring of fosinopril sodium impurities by liquid chromatography-mass spectrometry including the neural networks in method evaluation. in Journal of Chromatography A
Elsevier Science BV, Amsterdam., 1189(1-2), 366-373.
https://doi.org/10.1016/j.chroma.2007.11.076

Jančić B, Medenica M, Ivanović D, Janković S, Malenović A. Monitoring of fosinopril sodium impurities by liquid chromatography-mass spectrometry including the neural networks in method evaluation. in Journal of Chromatography A. 2008;1189(1-2):366-373.
doi:10.1016/j.chroma.2007.11.076 .

Jančić, Biljana, Medenica, Mirjana, Ivanović, D., Janković, Saša, Malenović, Anđelija, "Monitoring of fosinopril sodium impurities by liquid chromatography-mass spectrometry including the neural networks in method evaluation" in Journal of Chromatography A, 1189, no. 1-2 (2008):366-373,
https://doi.org/10.1016/j.chroma.2007.11.076 . .

TY  - JOUR
AU  - Malenović, Anđelija
AU  - Jančić-Stojanović, Biljana
AU  - Medenica, Mirjana
AU  - Ivanović, D.
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1058
AB  - This paper reports development and validation of a new microemulsion liquid chromatographic (MELC) method for rapid screening of simvastatin and simvastatin acid in human plasma. Plasma samples were injected directly into the HPLC system after appropriate sample dilution with mobile phase. Separations were performed on a 4.6 mm x 150 mm, 5-mu m particle, C-18 column, with UV detection at 238 nm. The mobile phase was 0.5% (w/v) diisopropyl ether, 1.0% (w/v) sodium dodecylsulphate (SDS), 4.0% (w/v) n-butanol, and 94.5% (w/w) aqueous 25 mM disodium hydrogen phosphate, pH 7.0, at a flow rate of 1 mL min(-1). The method was evaluated according to criteria stated in FDA bioanalytical method validation guidance. The unique approach applied in this paper enables direct analysis of simvastatin and simvastatin acid, so the method can be used to obtain reliable results in a rapid and simple way.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Chromatographica
T1  - Microemulsion Liquid Chromatographic Screening of Simvastatin and its Active Metabolite in Human Plasma
VL  - 20
IS  - 4
SP  - 595
EP  - 607
DO  - 10.1556/AChrom.20.2008.4.6
ER  - 

@article{
author = "Malenović, Anđelija and Jančić-Stojanović, Biljana and Medenica, Mirjana and Ivanović, D.",
year = "2008",
abstract = "This paper reports development and validation of a new microemulsion liquid chromatographic (MELC) method for rapid screening of simvastatin and simvastatin acid in human plasma. Plasma samples were injected directly into the HPLC system after appropriate sample dilution with mobile phase. Separations were performed on a 4.6 mm x 150 mm, 5-mu m particle, C-18 column, with UV detection at 238 nm. The mobile phase was 0.5% (w/v) diisopropyl ether, 1.0% (w/v) sodium dodecylsulphate (SDS), 4.0% (w/v) n-butanol, and 94.5% (w/w) aqueous 25 mM disodium hydrogen phosphate, pH 7.0, at a flow rate of 1 mL min(-1). The method was evaluated according to criteria stated in FDA bioanalytical method validation guidance. The unique approach applied in this paper enables direct analysis of simvastatin and simvastatin acid, so the method can be used to obtain reliable results in a rapid and simple way.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Chromatographica",
title = "Microemulsion Liquid Chromatographic Screening of Simvastatin and its Active Metabolite in Human Plasma",
volume = "20",
number = "4",
pages = "595-607",
doi = "10.1556/AChrom.20.2008.4.6"
}

Malenović, A., Jančić-Stojanović, B., Medenica, M.,& Ivanović, D.. (2008). Microemulsion Liquid Chromatographic Screening of Simvastatin and its Active Metabolite in Human Plasma. in Acta Chromatographica
Akademiai Kiado Zrt, Budapest., 20(4), 595-607.
https://doi.org/10.1556/AChrom.20.2008.4.6

Malenović A, Jančić-Stojanović B, Medenica M, Ivanović D. Microemulsion Liquid Chromatographic Screening of Simvastatin and its Active Metabolite in Human Plasma. in Acta Chromatographica. 2008;20(4):595-607.
doi:10.1556/AChrom.20.2008.4.6 .

Malenović, Anđelija, Jančić-Stojanović, Biljana, Medenica, Mirjana, Ivanović, D., "Microemulsion Liquid Chromatographic Screening of Simvastatin and its Active Metabolite in Human Plasma" in Acta Chromatographica, 20, no. 4 (2008):595-607,
https://doi.org/10.1556/AChrom.20.2008.4.6 . .

TY  - JOUR
AU  - Medenica, Mirjana
AU  - Ivanović, D.
AU  - Magković, M.
AU  - Jančić, Biljana
AU  - Malenović, Anđelija
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/968
AB  - Perindopril tert-butylamine is a new member of angiotensin-converting enzyme inhibitors group used in the treatment of hypertension and heart failure. In this paper, the evaluation of reversed-phase high-performance liquid chromatographic method (RP-HPLC) for the determination of impurities level of perindopril tert-butylamine in tablets was done. The chromatograms were recorded using a Hewlett Packard 1100 chromatographic system with DAD detector. Separations were performed on a YMC-Pack C8 column (250 mm x 4.6 mm; 5 mu m particle size) at 50 degrees C column temperature. Mobile phase was a mixture of acetonitrile-potassium phosphate buffer (0.05 M) (37:63, v/v) (pH 2.5). pH of the mobile phase was adjusted with ortophosphoric acid. Mixture of acetonitrile-water (40:60, v/v) was used as a solvent. Injection volume was 50 mu l, flow rate 1.7 ml min(-1) and UV-detection was performed at 215 nm. The developed method subjected to method validation and parameters in terms of selectivity, linearity, precision, accuracy, limit of detection, limit of quantitation and robustness were defined. The validated method is suitable for the simultaneous determination of perindopril tert-butylamine as well as its impurities in pharmaceuticals.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Evaluation of impurities level of perindopril tert-butylamine in tablets
VL  - 44
IS  - 5
SP  - 1087
EP  - 1094
DO  - 10.1016/j.jpba.2007.05.008
ER  - 

@article{
author = "Medenica, Mirjana and Ivanović, D. and Magković, M. and Jančić, Biljana and Malenović, Anđelija",
year = "2007",
abstract = "Perindopril tert-butylamine is a new member of angiotensin-converting enzyme inhibitors group used in the treatment of hypertension and heart failure. In this paper, the evaluation of reversed-phase high-performance liquid chromatographic method (RP-HPLC) for the determination of impurities level of perindopril tert-butylamine in tablets was done. The chromatograms were recorded using a Hewlett Packard 1100 chromatographic system with DAD detector. Separations were performed on a YMC-Pack C8 column (250 mm x 4.6 mm; 5 mu m particle size) at 50 degrees C column temperature. Mobile phase was a mixture of acetonitrile-potassium phosphate buffer (0.05 M) (37:63, v/v) (pH 2.5). pH of the mobile phase was adjusted with ortophosphoric acid. Mixture of acetonitrile-water (40:60, v/v) was used as a solvent. Injection volume was 50 mu l, flow rate 1.7 ml min(-1) and UV-detection was performed at 215 nm. The developed method subjected to method validation and parameters in terms of selectivity, linearity, precision, accuracy, limit of detection, limit of quantitation and robustness were defined. The validated method is suitable for the simultaneous determination of perindopril tert-butylamine as well as its impurities in pharmaceuticals.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Evaluation of impurities level of perindopril tert-butylamine in tablets",
volume = "44",
number = "5",
pages = "1087-1094",
doi = "10.1016/j.jpba.2007.05.008"
}

Medenica, M., Ivanović, D., Magković, M., Jančić, B.,& Malenović, A.. (2007). Evaluation of impurities level of perindopril tert-butylamine in tablets. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science BV, Amsterdam., 44(5), 1087-1094.
https://doi.org/10.1016/j.jpba.2007.05.008

Medenica M, Ivanović D, Magković M, Jančić B, Malenović A. Evaluation of impurities level of perindopril tert-butylamine in tablets. in Journal of Pharmaceutical and Biomedical Analysis. 2007;44(5):1087-1094.
doi:10.1016/j.jpba.2007.05.008 .

Medenica, Mirjana, Ivanović, D., Magković, M., Jančić, Biljana, Malenović, Anđelija, "Evaluation of impurities level of perindopril tert-butylamine in tablets" in Journal of Pharmaceutical and Biomedical Analysis, 44, no. 5 (2007):1087-1094,
https://doi.org/10.1016/j.jpba.2007.05.008 . .

TY  - JOUR
AU  - Ivanović, D.
AU  - Medenica, Mirjana
AU  - Jančić, Biljana
AU  - Knežević, N.
AU  - Malenović, Anđelija
AU  - Milić, J.
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/964
AB  - The main objective of the work discussed in this paper was evaluation of a chromatographic method for simultaneous determination of hydrochlorothiazide (HCTZ), lisinopril (L), and their impurities in pharmaceuticals. Chlorothiazide (CTZ) and disulfonamide (DSA), as potential impurities in hydrochlorothiazide, and diketopiperazine (DKP), as an impurity of lisinopril, were analyzed. The chromatographic behaviour of these substances on different columns was studied using mobile phases of different polarity. The optimum separations were achieved by gradient elution on a 4.6 mm x 20 mm, 3.5 mu m particle size, C-18 column. The mobile phase was a gradient prepared by mixing 7:93 (v/v) acetonitrile-25 mm potassium dihydrogen phosphate, pH 5, and 50:50 (v/v) acetonitrile-25 mm potassium dihydrogen phosphate pH 5 in different ratios. The flow rate was 1.0 mL min(-1). UV detection was performed at 215 nm. Methylparaben was used as internal standard. The method was validated for selectivity, linearity, precision,. and accuracy. The limits of detection, LOD, and quantification, LOQ, were determined experimentally. Because of its speed and accuracy the method can be used for quality-control analysis.
PB  - Univ Silesia, Inst Chemistry, Katowice
T2  - Acta Chromatographica
T1  - Validation of an analytical procedure for simultaneous determination of hydrochlorothiazide, lisinopril, and their impurities
VL  - 18
SP  - 143
EP  - 156
UR  - https://hdl.handle.net/21.15107/rcub_farfar_964
ER  - 

@article{
author = "Ivanović, D. and Medenica, Mirjana and Jančić, Biljana and Knežević, N. and Malenović, Anđelija and Milić, J.",
year = "2007",
abstract = "The main objective of the work discussed in this paper was evaluation of a chromatographic method for simultaneous determination of hydrochlorothiazide (HCTZ), lisinopril (L), and their impurities in pharmaceuticals. Chlorothiazide (CTZ) and disulfonamide (DSA), as potential impurities in hydrochlorothiazide, and diketopiperazine (DKP), as an impurity of lisinopril, were analyzed. The chromatographic behaviour of these substances on different columns was studied using mobile phases of different polarity. The optimum separations were achieved by gradient elution on a 4.6 mm x 20 mm, 3.5 mu m particle size, C-18 column. The mobile phase was a gradient prepared by mixing 7:93 (v/v) acetonitrile-25 mm potassium dihydrogen phosphate, pH 5, and 50:50 (v/v) acetonitrile-25 mm potassium dihydrogen phosphate pH 5 in different ratios. The flow rate was 1.0 mL min(-1). UV detection was performed at 215 nm. Methylparaben was used as internal standard. The method was validated for selectivity, linearity, precision,. and accuracy. The limits of detection, LOD, and quantification, LOQ, were determined experimentally. Because of its speed and accuracy the method can be used for quality-control analysis.",
publisher = "Univ Silesia, Inst Chemistry, Katowice",
journal = "Acta Chromatographica",
title = "Validation of an analytical procedure for simultaneous determination of hydrochlorothiazide, lisinopril, and their impurities",
volume = "18",
pages = "143-156",
url = "https://hdl.handle.net/21.15107/rcub_farfar_964"
}

Ivanović, D., Medenica, M., Jančić, B., Knežević, N., Malenović, A.,& Milić, J.. (2007). Validation of an analytical procedure for simultaneous determination of hydrochlorothiazide, lisinopril, and their impurities. in Acta Chromatographica
Univ Silesia, Inst Chemistry, Katowice., 18, 143-156.
https://hdl.handle.net/21.15107/rcub_farfar_964

Ivanović D, Medenica M, Jančić B, Knežević N, Malenović A, Milić J. Validation of an analytical procedure for simultaneous determination of hydrochlorothiazide, lisinopril, and their impurities. in Acta Chromatographica. 2007;18:143-156.
https://hdl.handle.net/21.15107/rcub_farfar_964 .

Ivanović, D., Medenica, Mirjana, Jančić, Biljana, Knežević, N., Malenović, Anđelija, Milić, J., "Validation of an analytical procedure for simultaneous determination of hydrochlorothiazide, lisinopril, and their impurities" in Acta Chromatographica, 18 (2007):143-156,
https://hdl.handle.net/21.15107/rcub_farfar_964 .

TY  - JOUR
AU  - Jančić, Biljana
AU  - Medenica, Mirjana
AU  - Ivanović, D.
AU  - Malenović, Anđelija
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/920
AB  - The main goal in this investigation was monocratic HPLC determination of dissociation constant values (pK(a)) of pramipexole and its impurities, BI-II 546 CL, BI-II 751 xx and 2-aminobenzothiazole. The chromatographic method is advantageous as a small amount of substance is needed and the degree of substance purity is less important. Analysis was carried out using stationary phases stable in a wide pH range. Triethylamonium phosphoric buffer was selected as appropriate pH controlling solution because it can cover a wide pH range. Detection was done on two different wavelengths, 262 nm for pramipexole, BI-II 751 xx and 2-aminobenzothiazole and at 326 nm for BI-II 546 CL. The constants were calculated from the typical sigmoidal curves of analyte obtained as retention factor versus the pH of the mobile phase.
PB  - Vieweg, Wiesbaden
T2  - Chromatographia
T1  - Chromatographic determination of dissociation constants of pramipexole and its impurities
VL  - 65
IS  - 9-10
SP  - 633
EP  - 635
DO  - 10.1365/s10337-007-0199-5
ER  - 

@article{
author = "Jančić, Biljana and Medenica, Mirjana and Ivanović, D. and Malenović, Anđelija",
year = "2007",
abstract = "The main goal in this investigation was monocratic HPLC determination of dissociation constant values (pK(a)) of pramipexole and its impurities, BI-II 546 CL, BI-II 751 xx and 2-aminobenzothiazole. The chromatographic method is advantageous as a small amount of substance is needed and the degree of substance purity is less important. Analysis was carried out using stationary phases stable in a wide pH range. Triethylamonium phosphoric buffer was selected as appropriate pH controlling solution because it can cover a wide pH range. Detection was done on two different wavelengths, 262 nm for pramipexole, BI-II 751 xx and 2-aminobenzothiazole and at 326 nm for BI-II 546 CL. The constants were calculated from the typical sigmoidal curves of analyte obtained as retention factor versus the pH of the mobile phase.",
publisher = "Vieweg, Wiesbaden",
journal = "Chromatographia",
title = "Chromatographic determination of dissociation constants of pramipexole and its impurities",
volume = "65",
number = "9-10",
pages = "633-635",
doi = "10.1365/s10337-007-0199-5"
}

Jančić, B., Medenica, M., Ivanović, D.,& Malenović, A.. (2007). Chromatographic determination of dissociation constants of pramipexole and its impurities. in Chromatographia
Vieweg, Wiesbaden., 65(9-10), 633-635.
https://doi.org/10.1365/s10337-007-0199-5

Jančić B, Medenica M, Ivanović D, Malenović A. Chromatographic determination of dissociation constants of pramipexole and its impurities. in Chromatographia. 2007;65(9-10):633-635.
doi:10.1365/s10337-007-0199-5 .

Jančić, Biljana, Medenica, Mirjana, Ivanović, D., Malenović, Anđelija, "Chromatographic determination of dissociation constants of pramipexole and its impurities" in Chromatographia, 65, no. 9-10 (2007):633-635,
https://doi.org/10.1365/s10337-007-0199-5 . .

TY  - CONF
AU  - Popović, Igor
AU  - Ivanović, D.
AU  - Medenica, Mirjana
AU  - Malenović, Anđelija
AU  - Jančić, Biljana
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/664
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - DryLab 2000 Plus® program in HPLC method optimization
T1  - Optimizacija HPLC metode primenom DryLab 2000 Plus® programa
VL  - 56
IS  - 5
SP  - 744
EP  - 745
UR  - https://hdl.handle.net/21.15107/rcub_farfar_664
ER  - 

@conference{
author = "Popović, Igor and Ivanović, D. and Medenica, Mirjana and Malenović, Anđelija and Jančić, Biljana",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "DryLab 2000 Plus® program in HPLC method optimization, Optimizacija HPLC metode primenom DryLab 2000 Plus® programa",
volume = "56",
number = "5",
pages = "744-745",
url = "https://hdl.handle.net/21.15107/rcub_farfar_664"
}

Popović, I., Ivanović, D., Medenica, M., Malenović, A.,& Jančić, B.. (2006). DryLab 2000 Plus® program in HPLC method optimization. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(5), 744-745.
https://hdl.handle.net/21.15107/rcub_farfar_664

Popović I, Ivanović D, Medenica M, Malenović A, Jančić B. DryLab 2000 Plus® program in HPLC method optimization. in Arhiv za farmaciju. 2006;56(5):744-745.
https://hdl.handle.net/21.15107/rcub_farfar_664 .

Popović, Igor, Ivanović, D., Medenica, Mirjana, Malenović, Anđelija, Jančić, Biljana, "DryLab 2000 Plus® program in HPLC method optimization" in Arhiv za farmaciju, 56, no. 5 (2006):744-745,
https://hdl.handle.net/21.15107/rcub_farfar_664 .

TY  - CONF
AU  - Jančić, Biljana
AU  - Medenica, Mirjana
AU  - Ivanović, D.
AU  - Malenović, Anđelija
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/700
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Monitoring of carbamazepine impurities using liquid chromatography
T1  - Određivanje nečistoća karbamazepina primenom tečne hromatografije
VL  - 56
IS  - 5
SP  - 742
EP  - 743
UR  - https://hdl.handle.net/21.15107/rcub_farfar_700
ER  - 

@conference{
author = "Jančić, Biljana and Medenica, Mirjana and Ivanović, D. and Malenović, Anđelija",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Monitoring of carbamazepine impurities using liquid chromatography, Određivanje nečistoća karbamazepina primenom tečne hromatografije",
volume = "56",
number = "5",
pages = "742-743",
url = "https://hdl.handle.net/21.15107/rcub_farfar_700"
}

Jančić, B., Medenica, M., Ivanović, D.,& Malenović, A.. (2006). Monitoring of carbamazepine impurities using liquid chromatography. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(5), 742-743.
https://hdl.handle.net/21.15107/rcub_farfar_700

Jančić B, Medenica M, Ivanović D, Malenović A. Monitoring of carbamazepine impurities using liquid chromatography. in Arhiv za farmaciju. 2006;56(5):742-743.
https://hdl.handle.net/21.15107/rcub_farfar_700 .

Jančić, Biljana, Medenica, Mirjana, Ivanović, D., Malenović, Anđelija, "Monitoring of carbamazepine impurities using liquid chromatography" in Arhiv za farmaciju, 56, no. 5 (2006):742-743,
https://hdl.handle.net/21.15107/rcub_farfar_700 .

TY  - CONF
AU  - Malenović, Anđelija
AU  - Ivanović, D.
AU  - Medenica, Mirjana
AU  - Jančić, Biljana
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/690
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Microemulsion liquid chomatography in analysis of some drugs and their metabolites in human plasma
T1  - Analiza nekih lekova i njihovih metabolita u humanoj plazmi primenom mikroemulzione tečne hromatografije
VL  - 56
IS  - 5
SP  - 740
EP  - 741
UR  - https://hdl.handle.net/21.15107/rcub_farfar_690
ER  - 

@conference{
author = "Malenović, Anđelija and Ivanović, D. and Medenica, Mirjana and Jančić, Biljana",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Microemulsion liquid chomatography in analysis of some drugs and their metabolites in human plasma, Analiza nekih lekova i njihovih metabolita u humanoj plazmi primenom mikroemulzione tečne hromatografije",
volume = "56",
number = "5",
pages = "740-741",
url = "https://hdl.handle.net/21.15107/rcub_farfar_690"
}

Malenović, A., Ivanović, D., Medenica, M.,& Jančić, B.. (2006). Microemulsion liquid chomatography in analysis of some drugs and their metabolites in human plasma. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(5), 740-741.
https://hdl.handle.net/21.15107/rcub_farfar_690

Malenović A, Ivanović D, Medenica M, Jančić B. Microemulsion liquid chomatography in analysis of some drugs and their metabolites in human plasma. in Arhiv za farmaciju. 2006;56(5):740-741.
https://hdl.handle.net/21.15107/rcub_farfar_690 .

Malenović, Anđelija, Ivanović, D., Medenica, Mirjana, Jančić, Biljana, "Microemulsion liquid chomatography in analysis of some drugs and their metabolites in human plasma" in Arhiv za farmaciju, 56, no. 5 (2006):740-741,
https://hdl.handle.net/21.15107/rcub_farfar_690 .

TY  - JOUR
AU  - Malenović, Anđelija
AU  - Medenica, Mirjana
AU  - Ivanović, D.
AU  - Jančić, Biljana
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/818
AB  - The use of microemulsions as eluents in HPLC has shown excellent potential. We have developed a novel approach for the analysis of Simvastatin and its six impurities, applying microemulsions as mobile phase. The method was validated and applied to the analysis of commercially available tablets in order to confirm that the proposed approach is useful for the application of this technique to drug analysis. A microemulsion eluent containing 0.9% w/w of diisopropylether, 1.7% w/w of sodium dodecyl-sulphate (SDS), 7.0% w/w of co-surfactant such as n-butanol and 90.4% w/w of aqueous 25 mM di-sodium phosphate pH 7.0 was used for the analysis. Separations were performed on a 3.5 mu m X Terra(TM) 50 x 4.6 mm column at 30 degrees C. Detection was performed at 238 nm with an eluent flow rate of 0.3 mL min(-1). The optimized and validated method can be used for the identification and simultaneous determination of Simvastatin and its six impurities in bulk drug and in pharmaceutical dosage forms.
PB  - Springer Heidelberg, Heidelberg
T2  - Chromatographia
T1  - Monitoring of Simvastatin impurities by HPLC with microemulsion eluents
VL  - 63
DO  - 10.1365/s10337-006-0747-4
ER  - 

@article{
author = "Malenović, Anđelija and Medenica, Mirjana and Ivanović, D. and Jančić, Biljana",
year = "2006",
abstract = "The use of microemulsions as eluents in HPLC has shown excellent potential. We have developed a novel approach for the analysis of Simvastatin and its six impurities, applying microemulsions as mobile phase. The method was validated and applied to the analysis of commercially available tablets in order to confirm that the proposed approach is useful for the application of this technique to drug analysis. A microemulsion eluent containing 0.9% w/w of diisopropylether, 1.7% w/w of sodium dodecyl-sulphate (SDS), 7.0% w/w of co-surfactant such as n-butanol and 90.4% w/w of aqueous 25 mM di-sodium phosphate pH 7.0 was used for the analysis. Separations were performed on a 3.5 mu m X Terra(TM) 50 x 4.6 mm column at 30 degrees C. Detection was performed at 238 nm with an eluent flow rate of 0.3 mL min(-1). The optimized and validated method can be used for the identification and simultaneous determination of Simvastatin and its six impurities in bulk drug and in pharmaceutical dosage forms.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Chromatographia",
title = "Monitoring of Simvastatin impurities by HPLC with microemulsion eluents",
volume = "63",
doi = "10.1365/s10337-006-0747-4"
}

Malenović, A., Medenica, M., Ivanović, D.,& Jančić, B.. (2006). Monitoring of Simvastatin impurities by HPLC with microemulsion eluents. in Chromatographia
Springer Heidelberg, Heidelberg., 63.
https://doi.org/10.1365/s10337-006-0747-4

Malenović A, Medenica M, Ivanović D, Jančić B. Monitoring of Simvastatin impurities by HPLC with microemulsion eluents. in Chromatographia. 2006;63.
doi:10.1365/s10337-006-0747-4 .

Malenović, Anđelija, Medenica, Mirjana, Ivanović, D., Jančić, Biljana, "Monitoring of Simvastatin impurities by HPLC with microemulsion eluents" in Chromatographia, 63 (2006),
https://doi.org/10.1365/s10337-006-0747-4 . .

TY  - JOUR
AU  - Malenović, Anđelija
AU  - Medenica, Mirjana
AU  - Ivanović, D
AU  - Jančić, Biljana
AU  - Marković, S
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/649
AB  - The simple and rapid RP-HPLC method, for the simultaneous determination of lidocaine and cetrimonium bromide in the presence of pellet color corrigent, was developed. Separations were performed on a Beckman Ultrasphere ODS 4.6 mm x 15 cm, 5 μm particle column at 40°C. The mobile phase consisted of water phase and acetonitrile (72:28:V/V), pH value of the mobile phase was adjusted to 2.0 with 85% ortophosphoric acid. Bisacodil was used as an internal standard. The flow rate was 1 ml/min and UV detection was performed at 208 nm. The proposed RP-HPLC method was validated and all the parameters for the validation of the method are given. According to the obtained results, the developed method was found to be suitable and accurate for the determination of these drugs in commercial formulations.
PB  - Elsevier Masson SAS
T2  - Farmaco
T1  - Development and validation of RP-HPLC method for cetrimonium bromide and lidocaine determination
VL  - 60
IS  - 2
SP  - 157
EP  - 161
DO  - 10.1016/j.farmac.2004.11.004
ER  - 

@article{
author = "Malenović, Anđelija and Medenica, Mirjana and Ivanović, D and Jančić, Biljana and Marković, S",
year = "2005",
abstract = "The simple and rapid RP-HPLC method, for the simultaneous determination of lidocaine and cetrimonium bromide in the presence of pellet color corrigent, was developed. Separations were performed on a Beckman Ultrasphere ODS 4.6 mm x 15 cm, 5 μm particle column at 40°C. The mobile phase consisted of water phase and acetonitrile (72:28:V/V), pH value of the mobile phase was adjusted to 2.0 with 85% ortophosphoric acid. Bisacodil was used as an internal standard. The flow rate was 1 ml/min and UV detection was performed at 208 nm. The proposed RP-HPLC method was validated and all the parameters for the validation of the method are given. According to the obtained results, the developed method was found to be suitable and accurate for the determination of these drugs in commercial formulations.",
publisher = "Elsevier Masson SAS",
journal = "Farmaco",
title = "Development and validation of RP-HPLC method for cetrimonium bromide and lidocaine determination",
volume = "60",
number = "2",
pages = "157-161",
doi = "10.1016/j.farmac.2004.11.004"
}

Malenović, A., Medenica, M., Ivanović, D., Jančić, B.,& Marković, S.. (2005). Development and validation of RP-HPLC method for cetrimonium bromide and lidocaine determination. in Farmaco
Elsevier Masson SAS., 60(2), 157-161.
https://doi.org/10.1016/j.farmac.2004.11.004

Malenović A, Medenica M, Ivanović D, Jančić B, Marković S. Development and validation of RP-HPLC method for cetrimonium bromide and lidocaine determination. in Farmaco. 2005;60(2):157-161.
doi:10.1016/j.farmac.2004.11.004 .

Malenović, Anđelija, Medenica, Mirjana, Ivanović, D, Jančić, Biljana, Marković, S, "Development and validation of RP-HPLC method for cetrimonium bromide and lidocaine determination" in Farmaco, 60, no. 2 (2005):157-161,
https://doi.org/10.1016/j.farmac.2004.11.004 . .

TY  - JOUR
AU  - Jančić, Biljana
AU  - Medenica, Mirjana
AU  - Ivanović, D
AU  - Malenović, Anđelija
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/617
AB  - Indinavir, an antiviral drug, is a member of the novel hydroxyaminopentane amides class of HIV-1 protease inhibitors. It is the active substance in capsules which contain 400 mg indinavir as the sulfate salt and degradation products, a lactone and cis-aminoindanol, arising as a result of hydrolysis of its amide bond (at most 1.5% of the lactone and 0.5% cis-aminoindanol). RP HPLC has been evaluated as a method for qualitative and a quantitative analysis of indinavir and its degradation products in capsules. During method development the effects of several stationary and mobile phases on the separation were investigated. Separations were performed on an X Terra RP18, 150 mm x 4.6 mm, 5 mu m particle size, column at 20 degrees C. The mobile phase was 35:65 (v/v) mixture of acetonitrile and an aqueous solution of sodium lauryl sulfate and triethylamine. UV detection was performed at 214 nm. Important statistical data were determined for validation of the selectivity/specificity, linearity, precision, robustness, limit of detection, and limit of quantitation of the method. The validated method was then used for determination of amounts of indinavir (recovery between 100.6 and, 103.1%) and compounds resulting from hydrolysis of its amide bond (the lactone, 0.265%, and cis-aminoindanol, 0.07%). The method is suitable for simultaneous determination of indinavir and its degradation products in pharmaceuticals and the bulk drug.
PB  - Springer Heidelberg, Heidelberg
T2  - Chromatographia
T1  - Evaluation of a liquid chromatographic method for analysis of Indinavir and degradation products arising from hydrolysis of its amide bond
VL  - 62
IS  - 5-6
SP  - 233
EP  - 238
DO  - 10.1365/s10337-005-0617-5
ER  - 

@article{
author = "Jančić, Biljana and Medenica, Mirjana and Ivanović, D and Malenović, Anđelija",
year = "2005",
abstract = "Indinavir, an antiviral drug, is a member of the novel hydroxyaminopentane amides class of HIV-1 protease inhibitors. It is the active substance in capsules which contain 400 mg indinavir as the sulfate salt and degradation products, a lactone and cis-aminoindanol, arising as a result of hydrolysis of its amide bond (at most 1.5% of the lactone and 0.5% cis-aminoindanol). RP HPLC has been evaluated as a method for qualitative and a quantitative analysis of indinavir and its degradation products in capsules. During method development the effects of several stationary and mobile phases on the separation were investigated. Separations were performed on an X Terra RP18, 150 mm x 4.6 mm, 5 mu m particle size, column at 20 degrees C. The mobile phase was 35:65 (v/v) mixture of acetonitrile and an aqueous solution of sodium lauryl sulfate and triethylamine. UV detection was performed at 214 nm. Important statistical data were determined for validation of the selectivity/specificity, linearity, precision, robustness, limit of detection, and limit of quantitation of the method. The validated method was then used for determination of amounts of indinavir (recovery between 100.6 and, 103.1%) and compounds resulting from hydrolysis of its amide bond (the lactone, 0.265%, and cis-aminoindanol, 0.07%). The method is suitable for simultaneous determination of indinavir and its degradation products in pharmaceuticals and the bulk drug.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Chromatographia",
title = "Evaluation of a liquid chromatographic method for analysis of Indinavir and degradation products arising from hydrolysis of its amide bond",
volume = "62",
number = "5-6",
pages = "233-238",
doi = "10.1365/s10337-005-0617-5"
}

Jančić, B., Medenica, M., Ivanović, D.,& Malenović, A.. (2005). Evaluation of a liquid chromatographic method for analysis of Indinavir and degradation products arising from hydrolysis of its amide bond. in Chromatographia
Springer Heidelberg, Heidelberg., 62(5-6), 233-238.
https://doi.org/10.1365/s10337-005-0617-5

Jančić B, Medenica M, Ivanović D, Malenović A. Evaluation of a liquid chromatographic method for analysis of Indinavir and degradation products arising from hydrolysis of its amide bond. in Chromatographia. 2005;62(5-6):233-238.
doi:10.1365/s10337-005-0617-5 .

Jančić, Biljana, Medenica, Mirjana, Ivanović, D, Malenović, Anđelija, "Evaluation of a liquid chromatographic method for analysis of Indinavir and degradation products arising from hydrolysis of its amide bond" in Chromatographia, 62, no. 5-6 (2005):233-238,
https://doi.org/10.1365/s10337-005-0617-5 . .

TY  - JOUR
AU  - Jančić, Biljana
AU  - Medenica, Mirjana
AU  - Ivanović, D
AU  - Malenović, Anđelija
AU  - Marković, S
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/609
AB  - The properties of the eluent are the essential factors governing the efficiency in the high-performance liquid chromatography (HPLC) method. A novel approach in retention modelling in the liquid chromatographic separation of fosinopril sodium and its degradation product, fosinoprilat, applying a microemulsion as the mobile phase, was used. The modifications of the mobile phase included the changes to the type of the lipophilic phase, the type and concentration of co-surfactant and surfactant, as well as the pH of the mobile phase. In this study, a full factorial 2(3) design, as the optimal method for screening of the experiment, was applied for selecting factors which had an influence on separation. Optimisation was done by a central composite design. An appropriate resolution with reasonable retention times was obtained with a microemulsion containing 0.9% w/w of cyclohexane, 2.2% w/w of sodium dodecyl sulphate (SDS), 8.0% w/w of n-butanol and 88.9% of aqueous 25 mM disodium phosphate, the pH of which was adjusted to 2.8 with 85% orthophosphoric acid. Separations were performed on an X-Terra 50-mmx4.6-mm, 3.5- mu m particle size column at 30 degrees C. UV detection was performed at 220 nm and with a flow rate of 0.3 mL min(-1). The established method was validated and applied for analysis of appropriate tablets. The proposed chromatographic procedure for the separation of fosinopril sodium and its degradation product is less expensive compared with the conventional reversed-phase HPLC method, as well as being simple and rapid. The optimised and validated method can be used for separation, identification and simultaneous determination of fosinopfil sodium and fosinoprilat in bulk drug and in pharmaceutical dose forms.
PB  - Springer Heidelberg, Heidelberg
T2  - Analytical and Bioanalytical Chemistry
T1  - Microemulsion liquid chromatographic method for characterisation of fosinopril sodium and fosinoprilat separation with chemometrical support
VL  - 383
IS  - 4
SP  - 687
EP  - 694
DO  - 10.1007/s00216-005-0074-x
ER  - 

@article{
author = "Jančić, Biljana and Medenica, Mirjana and Ivanović, D and Malenović, Anđelija and Marković, S",
year = "2005",
abstract = "The properties of the eluent are the essential factors governing the efficiency in the high-performance liquid chromatography (HPLC) method. A novel approach in retention modelling in the liquid chromatographic separation of fosinopril sodium and its degradation product, fosinoprilat, applying a microemulsion as the mobile phase, was used. The modifications of the mobile phase included the changes to the type of the lipophilic phase, the type and concentration of co-surfactant and surfactant, as well as the pH of the mobile phase. In this study, a full factorial 2(3) design, as the optimal method for screening of the experiment, was applied for selecting factors which had an influence on separation. Optimisation was done by a central composite design. An appropriate resolution with reasonable retention times was obtained with a microemulsion containing 0.9% w/w of cyclohexane, 2.2% w/w of sodium dodecyl sulphate (SDS), 8.0% w/w of n-butanol and 88.9% of aqueous 25 mM disodium phosphate, the pH of which was adjusted to 2.8 with 85% orthophosphoric acid. Separations were performed on an X-Terra 50-mmx4.6-mm, 3.5- mu m particle size column at 30 degrees C. UV detection was performed at 220 nm and with a flow rate of 0.3 mL min(-1). The established method was validated and applied for analysis of appropriate tablets. The proposed chromatographic procedure for the separation of fosinopril sodium and its degradation product is less expensive compared with the conventional reversed-phase HPLC method, as well as being simple and rapid. The optimised and validated method can be used for separation, identification and simultaneous determination of fosinopfil sodium and fosinoprilat in bulk drug and in pharmaceutical dose forms.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Analytical and Bioanalytical Chemistry",
title = "Microemulsion liquid chromatographic method for characterisation of fosinopril sodium and fosinoprilat separation with chemometrical support",
volume = "383",
number = "4",
pages = "687-694",
doi = "10.1007/s00216-005-0074-x"
}

Jančić, B., Medenica, M., Ivanović, D., Malenović, A.,& Marković, S.. (2005). Microemulsion liquid chromatographic method for characterisation of fosinopril sodium and fosinoprilat separation with chemometrical support. in Analytical and Bioanalytical Chemistry
Springer Heidelberg, Heidelberg., 383(4), 687-694.
https://doi.org/10.1007/s00216-005-0074-x

Jančić B, Medenica M, Ivanović D, Malenović A, Marković S. Microemulsion liquid chromatographic method for characterisation of fosinopril sodium and fosinoprilat separation with chemometrical support. in Analytical and Bioanalytical Chemistry. 2005;383(4):687-694.
doi:10.1007/s00216-005-0074-x .

Jančić, Biljana, Medenica, Mirjana, Ivanović, D, Malenović, Anđelija, Marković, S, "Microemulsion liquid chromatographic method for characterisation of fosinopril sodium and fosinoprilat separation with chemometrical support" in Analytical and Bioanalytical Chemistry, 383, no. 4 (2005):687-694,
https://doi.org/10.1007/s00216-005-0074-x . .

TY  - JOUR
AU  - Jančić, Biljana
AU  - Ivanović, D
AU  - Medenica, Mirjana
AU  - Malenović, Anđelija
AU  - Dimković, Nada
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/572
AB  - Fosinopril sodium presents a prodrug for the active angiotensin converting enzyme (ACE) inhibitor, fosinoprilat. The dual elimination of fosinoprilat by the liver and the kidney distinguishes fosinopril from other angiotensin converting enzyme inhibitors. Such ways of elimination are important for antihypertensive therapy of patients on haemodialysis. The paper presents development and evaluation of a new and sensitive liquid chromatographic (LC) method for the analysis of fosinoprilat in plasma obtained from patients on haemodialysis. A microemulsion system mixture as mobile phase has been used for the separation and analysis of fosinoprilat in plasma samples. The plasma samples were injected directly onto the HPLC system (Waters Breeze) after appropriate sample dilution with mobile phase. Separations were performed on the Bakerbond ENV 4.6 mm x 150 mm, 5 mu m particle size column with UV detection at 220 nm. The flow rate was 1.00 mL min(-1). The mobile phase consisted of 1.0% (w/v) of diisopropyl ether, 2.0% (w/v) of sodium dodecyl sulphate (SDS), 6.0% (w/v) of n-propanol and 91 % (w/v) of aqueous 25 mM di-sodium hydrogen phosphate, pH adjusted to 2.8 with 85% orthophosphoric acid. The developed method was then subjected to method validation according to the criteria stated in the FDA bioanalytical method validation guidance. The results for specificity, linearity, low limit of quantification (LLOQ), precision, accuracy and stability were within the accepted criteria. The unique approach applied in this paper makes possible the determination of fosinoprilat even in the presence of metabolites of other drugs, so the method can be used for obtaining the reliable results in a fast and simple way.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Chromatography A
T1  - Development of liquid chromatographic method for fosinoprilat determination in human plasma using microemulsion as eluent
VL  - 1088
IS  - 1-2
SP  - 187
EP  - 192
DO  - 10.1016/j.chroma.2005.05.038
ER  - 

@article{
author = "Jančić, Biljana and Ivanović, D and Medenica, Mirjana and Malenović, Anđelija and Dimković, Nada",
year = "2005",
abstract = "Fosinopril sodium presents a prodrug for the active angiotensin converting enzyme (ACE) inhibitor, fosinoprilat. The dual elimination of fosinoprilat by the liver and the kidney distinguishes fosinopril from other angiotensin converting enzyme inhibitors. Such ways of elimination are important for antihypertensive therapy of patients on haemodialysis. The paper presents development and evaluation of a new and sensitive liquid chromatographic (LC) method for the analysis of fosinoprilat in plasma obtained from patients on haemodialysis. A microemulsion system mixture as mobile phase has been used for the separation and analysis of fosinoprilat in plasma samples. The plasma samples were injected directly onto the HPLC system (Waters Breeze) after appropriate sample dilution with mobile phase. Separations were performed on the Bakerbond ENV 4.6 mm x 150 mm, 5 mu m particle size column with UV detection at 220 nm. The flow rate was 1.00 mL min(-1). The mobile phase consisted of 1.0% (w/v) of diisopropyl ether, 2.0% (w/v) of sodium dodecyl sulphate (SDS), 6.0% (w/v) of n-propanol and 91 % (w/v) of aqueous 25 mM di-sodium hydrogen phosphate, pH adjusted to 2.8 with 85% orthophosphoric acid. The developed method was then subjected to method validation according to the criteria stated in the FDA bioanalytical method validation guidance. The results for specificity, linearity, low limit of quantification (LLOQ), precision, accuracy and stability were within the accepted criteria. The unique approach applied in this paper makes possible the determination of fosinoprilat even in the presence of metabolites of other drugs, so the method can be used for obtaining the reliable results in a fast and simple way.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Chromatography A",
title = "Development of liquid chromatographic method for fosinoprilat determination in human plasma using microemulsion as eluent",
volume = "1088",
number = "1-2",
pages = "187-192",
doi = "10.1016/j.chroma.2005.05.038"
}

Jančić, B., Ivanović, D., Medenica, M., Malenović, A.,& Dimković, N.. (2005). Development of liquid chromatographic method for fosinoprilat determination in human plasma using microemulsion as eluent. in Journal of Chromatography A
Elsevier Science BV, Amsterdam., 1088(1-2), 187-192.
https://doi.org/10.1016/j.chroma.2005.05.038

Jančić B, Ivanović D, Medenica M, Malenović A, Dimković N. Development of liquid chromatographic method for fosinoprilat determination in human plasma using microemulsion as eluent. in Journal of Chromatography A. 2005;1088(1-2):187-192.
doi:10.1016/j.chroma.2005.05.038 .

Jančić, Biljana, Ivanović, D, Medenica, Mirjana, Malenović, Anđelija, Dimković, Nada, "Development of liquid chromatographic method for fosinoprilat determination in human plasma using microemulsion as eluent" in Journal of Chromatography A, 1088, no. 1-2 (2005):187-192,
https://doi.org/10.1016/j.chroma.2005.05.038 . .

TY  - JOUR
AU  - Malenović, Anđelija
AU  - Ivanović, Darko
AU  - Medenica, Mirjana
AU  - Jančić, Biljana
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/522
AB  - The successful analysis of drugs in complex pharmaceutical formulations by reversed-phase high performance liquid chromatography (RP-HPLC) relies upon the optimization of the chromatographic conditions, the sample preparation and post-column detection. HPLC separation of mixtures depends on a large number of factors. The optimization of RP-HPLC method defines the simultaneous influence of some important conditions, such as column type, mobile phase composition, pH of the mobile phase, column temperature and flow rate, to the separation and determination. In this paper the RP-HPLC method was applied for the optimal separation of lidocaine, cetrimonium bromide and color in the pharmaceutical pellets (Septalen(R) pellets). The selectivity factor values defined the optimal conditions, which were confirmed by analyzing the appropriate mathematical models. With the aid of the response surface methodology (RSM) it was possible to anticipate to a certain degree and precisely select optimal experimental conditions. Separations were performed on a Beckman Ultrasphere ODS 4.6 x 150 mm, 5 mum particle size column. Samples were introduced through a Rheodyne injector valve with a 20 muL sample loop. UV detection was performed at 208 nm. The optimization was performed within the pH range from 2.0 to 5.5; temperature range from 20 degrees C to 55 degrees C and composition of the mobile phase acetonitrile water from (15:85 V/V) to (35:65 V/V). The three-D graphs, constructed with sixty-four experimental points, confirmed the optimal conditions for the separation of lidocaine, cetrimonium bromide and color in analyzed pharmaceutical preparations.
PB  - Slovensko Kemijsko Drustvo, Ljubljana
T2  - Acta Chimica Slovenica
T1  - Application of the response surface methodology for RP-HPLC analysis of lidocaine and cetrimonium bromide
VL  - 51
IS  - 3
SP  - 559
EP  - 566
UR  - https://hdl.handle.net/21.15107/rcub_farfar_522
ER  - 

@article{
author = "Malenović, Anđelija and Ivanović, Darko and Medenica, Mirjana and Jančić, Biljana",
year = "2004",
abstract = "The successful analysis of drugs in complex pharmaceutical formulations by reversed-phase high performance liquid chromatography (RP-HPLC) relies upon the optimization of the chromatographic conditions, the sample preparation and post-column detection. HPLC separation of mixtures depends on a large number of factors. The optimization of RP-HPLC method defines the simultaneous influence of some important conditions, such as column type, mobile phase composition, pH of the mobile phase, column temperature and flow rate, to the separation and determination. In this paper the RP-HPLC method was applied for the optimal separation of lidocaine, cetrimonium bromide and color in the pharmaceutical pellets (Septalen(R) pellets). The selectivity factor values defined the optimal conditions, which were confirmed by analyzing the appropriate mathematical models. With the aid of the response surface methodology (RSM) it was possible to anticipate to a certain degree and precisely select optimal experimental conditions. Separations were performed on a Beckman Ultrasphere ODS 4.6 x 150 mm, 5 mum particle size column. Samples were introduced through a Rheodyne injector valve with a 20 muL sample loop. UV detection was performed at 208 nm. The optimization was performed within the pH range from 2.0 to 5.5; temperature range from 20 degrees C to 55 degrees C and composition of the mobile phase acetonitrile water from (15:85 V/V) to (35:65 V/V). The three-D graphs, constructed with sixty-four experimental points, confirmed the optimal conditions for the separation of lidocaine, cetrimonium bromide and color in analyzed pharmaceutical preparations.",
publisher = "Slovensko Kemijsko Drustvo, Ljubljana",
journal = "Acta Chimica Slovenica",
title = "Application of the response surface methodology for RP-HPLC analysis of lidocaine and cetrimonium bromide",
volume = "51",
number = "3",
pages = "559-566",
url = "https://hdl.handle.net/21.15107/rcub_farfar_522"
}

Malenović, A., Ivanović, D., Medenica, M.,& Jančić, B.. (2004). Application of the response surface methodology for RP-HPLC analysis of lidocaine and cetrimonium bromide. in Acta Chimica Slovenica
Slovensko Kemijsko Drustvo, Ljubljana., 51(3), 559-566.
https://hdl.handle.net/21.15107/rcub_farfar_522

Malenović A, Ivanović D, Medenica M, Jančić B. Application of the response surface methodology for RP-HPLC analysis of lidocaine and cetrimonium bromide. in Acta Chimica Slovenica. 2004;51(3):559-566.
https://hdl.handle.net/21.15107/rcub_farfar_522 .

Malenović, Anđelija, Ivanović, Darko, Medenica, Mirjana, Jančić, Biljana, "Application of the response surface methodology for RP-HPLC analysis of lidocaine and cetrimonium bromide" in Acta Chimica Slovenica, 51, no. 3 (2004):559-566,
https://hdl.handle.net/21.15107/rcub_farfar_522 .

TY  - JOUR
AU  - Medenica, Mirjana
AU  - Ivanović, D
AU  - Marković, S
AU  - Malenović, Anđelija
AU  - Jančić, Biljana
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/487
AB  - In this paper the second-derivative spectrophotometric method for the simultaneous determination of lidocaine (CAS 137-58-6) and hydrocortisone acetate (CAS 50-03-3) in suppositories is described. One suppository contains 60 mg of lidocaine and 5 mg of hydrocortisone acetate. Optimal conditions for the quantitative analysis were settled. The second-derivative spectra were recorded in the range from 210 to 380 nm against methanol. Determination of lidocaine was performed at 256.0 nm and hydrocortisone acetate at 260.5 nm, using the "zero crossing" method. The results obtained show that there was no interference of ingredients matrix under the mentioned experimental conditions. The method is simple, rapid and precise and can be used in a routine analysis of such pharmaceutical dosage forms.
PB  - ECV-Editio Cantor Verlag Medizin Naturwissenschaften, Aulendorf
T2  - Pharmazeutische Industrie
T1  - Second-derivative spectrophotometric analysis of lidocaine and hydrocortisone acetate in suppositories
VL  - 66
IS  - 3
SP  - 330
EP  - 333
UR  - https://hdl.handle.net/21.15107/rcub_farfar_487
ER  - 

@article{
author = "Medenica, Mirjana and Ivanović, D and Marković, S and Malenović, Anđelija and Jančić, Biljana",
year = "2004",
abstract = "In this paper the second-derivative spectrophotometric method for the simultaneous determination of lidocaine (CAS 137-58-6) and hydrocortisone acetate (CAS 50-03-3) in suppositories is described. One suppository contains 60 mg of lidocaine and 5 mg of hydrocortisone acetate. Optimal conditions for the quantitative analysis were settled. The second-derivative spectra were recorded in the range from 210 to 380 nm against methanol. Determination of lidocaine was performed at 256.0 nm and hydrocortisone acetate at 260.5 nm, using the "zero crossing" method. The results obtained show that there was no interference of ingredients matrix under the mentioned experimental conditions. The method is simple, rapid and precise and can be used in a routine analysis of such pharmaceutical dosage forms.",
publisher = "ECV-Editio Cantor Verlag Medizin Naturwissenschaften, Aulendorf",
journal = "Pharmazeutische Industrie",
title = "Second-derivative spectrophotometric analysis of lidocaine and hydrocortisone acetate in suppositories",
volume = "66",
number = "3",
pages = "330-333",
url = "https://hdl.handle.net/21.15107/rcub_farfar_487"
}

Medenica, M., Ivanović, D., Marković, S., Malenović, A.,& Jančić, B.. (2004). Second-derivative spectrophotometric analysis of lidocaine and hydrocortisone acetate in suppositories. in Pharmazeutische Industrie
ECV-Editio Cantor Verlag Medizin Naturwissenschaften, Aulendorf., 66(3), 330-333.
https://hdl.handle.net/21.15107/rcub_farfar_487

Medenica M, Ivanović D, Marković S, Malenović A, Jančić B. Second-derivative spectrophotometric analysis of lidocaine and hydrocortisone acetate in suppositories. in Pharmazeutische Industrie. 2004;66(3):330-333.
https://hdl.handle.net/21.15107/rcub_farfar_487 .

Medenica, Mirjana, Ivanović, D, Marković, S, Malenović, Anđelija, Jančić, Biljana, "Second-derivative spectrophotometric analysis of lidocaine and hydrocortisone acetate in suppositories" in Pharmazeutische Industrie, 66, no. 3 (2004):330-333,
https://hdl.handle.net/21.15107/rcub_farfar_487 .

TY  - JOUR
AU  - Ivanović, D
AU  - Medenica, Mirjana
AU  - Jančić, Biljana
AU  - Malenović, Anđelija
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/544
AB  - The reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for the simultaneous determination of imatinib mesylate and of the impurity product in Glivec(R) capsules (Novartis, Switzerland). Separations were performed on a X Terra(TM) 150 mm 4.6 mm, 5 mum particle size column at 25 degreesC. The mobile phase was a mixture of methanol-water-triethylamine (25:74: 1, v/v/v) with flow rate of 1.0 ml min(-1). pH value of water-triethylamine (TEA) was adjusted to 2.4 with orthophosphoric acid before adding of methanol. UV detection was performed at 267 nm. Acetaminophen was used as an internal standard. The method was validated statistically for its selectivity, linearity, precision, accuracy and robustness. Due to its speed and accuracy, the method may be used for quality control analyses.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
T1  - Reversed-phase liquid chromatography analysis of imatinib mesylate and impurity product in Glivec (R) capsules
VL  - 800
IS  - 1-2
SP  - 253
EP  - 258
DO  - 10.1016/j.jchromb.2003.10.018
ER  - 

@article{
author = "Ivanović, D and Medenica, Mirjana and Jančić, Biljana and Malenović, Anđelija",
year = "2004",
abstract = "The reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for the simultaneous determination of imatinib mesylate and of the impurity product in Glivec(R) capsules (Novartis, Switzerland). Separations were performed on a X Terra(TM) 150 mm 4.6 mm, 5 mum particle size column at 25 degreesC. The mobile phase was a mixture of methanol-water-triethylamine (25:74: 1, v/v/v) with flow rate of 1.0 ml min(-1). pH value of water-triethylamine (TEA) was adjusted to 2.4 with orthophosphoric acid before adding of methanol. UV detection was performed at 267 nm. Acetaminophen was used as an internal standard. The method was validated statistically for its selectivity, linearity, precision, accuracy and robustness. Due to its speed and accuracy, the method may be used for quality control analyses.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences",
title = "Reversed-phase liquid chromatography analysis of imatinib mesylate and impurity product in Glivec (R) capsules",
volume = "800",
number = "1-2",
pages = "253-258",
doi = "10.1016/j.jchromb.2003.10.018"
}

Ivanović, D., Medenica, M., Jančić, B.,& Malenović, A.. (2004). Reversed-phase liquid chromatography analysis of imatinib mesylate and impurity product in Glivec (R) capsules. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Elsevier Science BV, Amsterdam., 800(1-2), 253-258.
https://doi.org/10.1016/j.jchromb.2003.10.018

Ivanović D, Medenica M, Jančić B, Malenović A. Reversed-phase liquid chromatography analysis of imatinib mesylate and impurity product in Glivec (R) capsules. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. 2004;800(1-2):253-258.
doi:10.1016/j.jchromb.2003.10.018 .

Ivanović, D, Medenica, Mirjana, Jančić, Biljana, Malenović, Anđelija, "Reversed-phase liquid chromatography analysis of imatinib mesylate and impurity product in Glivec (R) capsules" in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 800, no. 1-2 (2004):253-258,
https://doi.org/10.1016/j.jchromb.2003.10.018 . .

TY  - JOUR
AU  - Ivanović, D
AU  - Medenica, Mirjana
AU  - Malenović, Anđelija
AU  - Jančić, Biljana
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/533
AB  - A rapid and sensitive reverse-phase high performance liquid chromatography (RP-HPLC) method with ultra-violet (UV) detection for a routine control of hydrochlorothiazide and captopril in tablets was developed. The chromatographic system Hewlet Packard 1100 consisted of a HP 1100 pump, HP 1100 UV-VIS detector and HP ChemStation integrator. The samples were introduced through a Rheodyne injector valve with a 20-muL sample loop. The isocratic system consisted of a Beckman Ultrasphere ODS 4.6 mm x 15 cm, 5-mum-particle column and a mobile phase containing methanol/water (45:55 v/v). The pH of the mobile phase was adjusted to 3.8 with 85% ortophosphoric acid. Quantitation was accomplished using the internal standard method. At the selected conditions, the other excipients of the tablets did not interfere in the assay of active substances. The developed RP-HPLC method was validated, so linearity, precision, accuracy, robustness, limit of quantitation and limit of detection were investigated. For the robustness test, three factors were considered: the composition of the mobile phase, the pH of the mobile phase, and temperature. With the aid of response surface metodology (RSM), it was possible to precisely define the robustness of the method.
PB  - Springer, New York
T2  - Accreditation and Quality Assurance
T1  - Validation of the RP-HPLC method for analysis of hydrochlorothiazide and captopril in tablets
VL  - 9
IS  - 1-2
SP  - 76
EP  - 81
DO  - 10.1007/s00769-003-0722-9
ER  - 

@article{
author = "Ivanović, D and Medenica, Mirjana and Malenović, Anđelija and Jančić, Biljana",
year = "2004",
abstract = "A rapid and sensitive reverse-phase high performance liquid chromatography (RP-HPLC) method with ultra-violet (UV) detection for a routine control of hydrochlorothiazide and captopril in tablets was developed. The chromatographic system Hewlet Packard 1100 consisted of a HP 1100 pump, HP 1100 UV-VIS detector and HP ChemStation integrator. The samples were introduced through a Rheodyne injector valve with a 20-muL sample loop. The isocratic system consisted of a Beckman Ultrasphere ODS 4.6 mm x 15 cm, 5-mum-particle column and a mobile phase containing methanol/water (45:55 v/v). The pH of the mobile phase was adjusted to 3.8 with 85% ortophosphoric acid. Quantitation was accomplished using the internal standard method. At the selected conditions, the other excipients of the tablets did not interfere in the assay of active substances. The developed RP-HPLC method was validated, so linearity, precision, accuracy, robustness, limit of quantitation and limit of detection were investigated. For the robustness test, three factors were considered: the composition of the mobile phase, the pH of the mobile phase, and temperature. With the aid of response surface metodology (RSM), it was possible to precisely define the robustness of the method.",
publisher = "Springer, New York",
journal = "Accreditation and Quality Assurance",
title = "Validation of the RP-HPLC method for analysis of hydrochlorothiazide and captopril in tablets",
volume = "9",
number = "1-2",
pages = "76-81",
doi = "10.1007/s00769-003-0722-9"
}

Ivanović, D., Medenica, M., Malenović, A.,& Jančić, B.. (2004). Validation of the RP-HPLC method for analysis of hydrochlorothiazide and captopril in tablets. in Accreditation and Quality Assurance
Springer, New York., 9(1-2), 76-81.
https://doi.org/10.1007/s00769-003-0722-9

Ivanović D, Medenica M, Malenović A, Jančić B. Validation of the RP-HPLC method for analysis of hydrochlorothiazide and captopril in tablets. in Accreditation and Quality Assurance. 2004;9(1-2):76-81.
doi:10.1007/s00769-003-0722-9 .

Ivanović, D, Medenica, Mirjana, Malenović, Anđelija, Jančić, Biljana, "Validation of the RP-HPLC method for analysis of hydrochlorothiazide and captopril in tablets" in Accreditation and Quality Assurance, 9, no. 1-2 (2004):76-81,
https://doi.org/10.1007/s00769-003-0722-9 . .

TY  - JOUR
AU  - Ivanović, D
AU  - Medenica, Mirjana
AU  - Jančić, Biljana
AU  - Malenović, Anđelija
AU  - Marković, S
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/509
AB  - In this paper the experimental design has been applied to define the optimum chromatographic conditions for the separation of Fosinopril sodium and its degradation product, fosinoprilat. Fosinopril is a prodrug and after being hydrolysed, it becomes an active drug fosinoprilat. For experimental screening full factorial design 2 3 was applied. Methanol content, PH of the mobile phase and column temperature were independent variables or factors to be investigated in two levels - much greater thanlowmuch less than and much greater thanhighmuch less than. Capacity and selectivity factors were chosen as dependent variables and matrix was applied to estimate coefficients of the linear model. After experimental screening, RSM (response surface methodology) was applied for optimization. Optimum conditions were: X Terra(TM) 150 mm x 4.6 mm, 5 mum particle column at 45 degreesC; methanol-water (75 : 25 v/v) at pH 3.1 as a mobile Phase, with a flow rate of 1 mL min(-1). UV detection was performed at 220 nm. Propylparaben was used as an internal standard. The proposed method is rapid, accurate, selective and because of its sensitivity and reproducibility, it may be used for the quantitative analysis of fosinopril sodium and its degradation product in Monopril(R) tablets. Recovery values for fosinopril sodium were between 101.6% and 102.9% and content of degradation product fosinoprilat was lower than 5%. Applying the chemometrical approach enables a relatively limited number of experiments to define factors which affect the chromatographic behavior of investigated substances and obtain optimum conditions for their analysis.
PB  - Springer Heidelberg, Heidelberg
T2  - Chromatographia
T1  - Chemometrical approach in fosinopril-sodium and its degradation product fosinoprilat analysis
VL  - 60
IS  - SUPPL.
DO  - 10.1365/s10337-004-0324-7
ER  - 

@article{
author = "Ivanović, D and Medenica, Mirjana and Jančić, Biljana and Malenović, Anđelija and Marković, S",
year = "2004",
abstract = "In this paper the experimental design has been applied to define the optimum chromatographic conditions for the separation of Fosinopril sodium and its degradation product, fosinoprilat. Fosinopril is a prodrug and after being hydrolysed, it becomes an active drug fosinoprilat. For experimental screening full factorial design 2 3 was applied. Methanol content, PH of the mobile phase and column temperature were independent variables or factors to be investigated in two levels - much greater thanlowmuch less than and much greater thanhighmuch less than. Capacity and selectivity factors were chosen as dependent variables and matrix was applied to estimate coefficients of the linear model. After experimental screening, RSM (response surface methodology) was applied for optimization. Optimum conditions were: X Terra(TM) 150 mm x 4.6 mm, 5 mum particle column at 45 degreesC; methanol-water (75 : 25 v/v) at pH 3.1 as a mobile Phase, with a flow rate of 1 mL min(-1). UV detection was performed at 220 nm. Propylparaben was used as an internal standard. The proposed method is rapid, accurate, selective and because of its sensitivity and reproducibility, it may be used for the quantitative analysis of fosinopril sodium and its degradation product in Monopril(R) tablets. Recovery values for fosinopril sodium were between 101.6% and 102.9% and content of degradation product fosinoprilat was lower than 5%. Applying the chemometrical approach enables a relatively limited number of experiments to define factors which affect the chromatographic behavior of investigated substances and obtain optimum conditions for their analysis.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Chromatographia",
title = "Chemometrical approach in fosinopril-sodium and its degradation product fosinoprilat analysis",
volume = "60",
number = "SUPPL.",
doi = "10.1365/s10337-004-0324-7"
}

Ivanović, D., Medenica, M., Jančić, B., Malenović, A.,& Marković, S.. (2004). Chemometrical approach in fosinopril-sodium and its degradation product fosinoprilat analysis. in Chromatographia
Springer Heidelberg, Heidelberg., 60(SUPPL.).
https://doi.org/10.1365/s10337-004-0324-7

Ivanović D, Medenica M, Jančić B, Malenović A, Marković S. Chemometrical approach in fosinopril-sodium and its degradation product fosinoprilat analysis. in Chromatographia. 2004;60(SUPPL.).
doi:10.1365/s10337-004-0324-7 .

Ivanović, D, Medenica, Mirjana, Jančić, Biljana, Malenović, Anđelija, Marković, S, "Chemometrical approach in fosinopril-sodium and its degradation product fosinoprilat analysis" in Chromatographia, 60, no. SUPPL. (2004),
https://doi.org/10.1365/s10337-004-0324-7 . .