TY  - JOUR
AU  - Tubić, Biljana
AU  - Marković, Bojan
AU  - Vladimirov, S.
AU  - Savić, Snežana
AU  - Poljarević, Jelena
AU  - Sabo, Tibor
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2796
AB  - Fourteen compounds representing ester derivatives of (S,S)-1,2-ethanediamine-N,N'-di-2-(3-cyclohexyl) propanoic and (S,S)-1,3-propanediamine-N,N'-di-2-(3-cyclohexyl)propanoic acids, expressing antiproliferative activity in vitro were examined. The objective of this study was to determinate their lipophilicity data, and also to ensure a mathematical model for prediction lipophilicity data of potential in vivo metabolites and new derivatives of (S,S)-1,2-ethanediannine-N,N'-di-2-(3-cyclohexyl)propanoic acid, based on chromatographic parameters. Experimentally, lipophilicity data were obtained by a traditional shake flask procedure and an ultra-high performance liquid chromatographic tandem mass spectrometry (UHPLC-MS) method. A correlation between the partition coefficient n-octanol/water (logD(7,4)) and chromatographic data (CHI, phi(0)), and also, between logD(7,4) and retention time was investigated. A very good correlation (r(2)=0.8969) was found between lipophilicity parameters phi(0) and logD(7,4) obtained using UHPLC-MS and shake flask methods: logD(7,4) = (0.11 +/- 0.01)x phi(0) + (1.25 +/- 0.20)xN(c) - (9.19 +/- 1.18); statistical parameter F=47.84; significance of F = 3.74x10(-6), N-c=number of C atoms between two amino groups (N-c=2 for 1,2-ethanediamine derivatives and N-c=3 for 1,3-propanediamine derivatives).The model predictivity power was determined by cross validation leave one out (LOO) technique, and expressed by the term Q(2), was 0.89. The developed model has good predictivity power for prediction lipophilicity data of potential in vivo metabolites of the investigated compounds, such as novel 1,2-ethanediamine and 1,3-propanediamine N,N'-di-2-(3-cyclohexyl)propanoic acid derivatives. Also, the lipophilicity data obtained in the present study correlated with the antiproliferative activity of the investigated substances shown previously in in vitro studies.
PB  - Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn
T2  - Pharmazie
T1  - A new model to determine lipophilicity of 1,2-ethanediamine-N,N'-di-2-(3-cyclohexyl)propanoic acid and 1,3-propanediamine-N,N'-di-2-(3-cyclohexyl)propanoic acid derivatives with antiproliferative activity by combining shake flask procedure and UHPLC-MS me
VL  - 72
IS  - 6
SP  - 317
EP  - 323
DO  - 10.1619/ph.2017.6208
ER  - 

@article{
author = "Tubić, Biljana and Marković, Bojan and Vladimirov, S. and Savić, Snežana and Poljarević, Jelena and Sabo, Tibor",
year = "2017",
abstract = "Fourteen compounds representing ester derivatives of (S,S)-1,2-ethanediamine-N,N'-di-2-(3-cyclohexyl) propanoic and (S,S)-1,3-propanediamine-N,N'-di-2-(3-cyclohexyl)propanoic acids, expressing antiproliferative activity in vitro were examined. The objective of this study was to determinate their lipophilicity data, and also to ensure a mathematical model for prediction lipophilicity data of potential in vivo metabolites and new derivatives of (S,S)-1,2-ethanediannine-N,N'-di-2-(3-cyclohexyl)propanoic acid, based on chromatographic parameters. Experimentally, lipophilicity data were obtained by a traditional shake flask procedure and an ultra-high performance liquid chromatographic tandem mass spectrometry (UHPLC-MS) method. A correlation between the partition coefficient n-octanol/water (logD(7,4)) and chromatographic data (CHI, phi(0)), and also, between logD(7,4) and retention time was investigated. A very good correlation (r(2)=0.8969) was found between lipophilicity parameters phi(0) and logD(7,4) obtained using UHPLC-MS and shake flask methods: logD(7,4) = (0.11 +/- 0.01)x phi(0) + (1.25 +/- 0.20)xN(c) - (9.19 +/- 1.18); statistical parameter F=47.84; significance of F = 3.74x10(-6), N-c=number of C atoms between two amino groups (N-c=2 for 1,2-ethanediamine derivatives and N-c=3 for 1,3-propanediamine derivatives).The model predictivity power was determined by cross validation leave one out (LOO) technique, and expressed by the term Q(2), was 0.89. The developed model has good predictivity power for prediction lipophilicity data of potential in vivo metabolites of the investigated compounds, such as novel 1,2-ethanediamine and 1,3-propanediamine N,N'-di-2-(3-cyclohexyl)propanoic acid derivatives. Also, the lipophilicity data obtained in the present study correlated with the antiproliferative activity of the investigated substances shown previously in in vitro studies.",
publisher = "Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn",
journal = "Pharmazie",
title = "A new model to determine lipophilicity of 1,2-ethanediamine-N,N'-di-2-(3-cyclohexyl)propanoic acid and 1,3-propanediamine-N,N'-di-2-(3-cyclohexyl)propanoic acid derivatives with antiproliferative activity by combining shake flask procedure and UHPLC-MS me",
volume = "72",
number = "6",
pages = "317-323",
doi = "10.1619/ph.2017.6208"
}

Tubić, B., Marković, B., Vladimirov, S., Savić, S., Poljarević, J.,& Sabo, T.. (2017). A new model to determine lipophilicity of 1,2-ethanediamine-N,N'-di-2-(3-cyclohexyl)propanoic acid and 1,3-propanediamine-N,N'-di-2-(3-cyclohexyl)propanoic acid derivatives with antiproliferative activity by combining shake flask procedure and UHPLC-MS me. in Pharmazie
Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn., 72(6), 317-323.
https://doi.org/10.1619/ph.2017.6208

Tubić B, Marković B, Vladimirov S, Savić S, Poljarević J, Sabo T. A new model to determine lipophilicity of 1,2-ethanediamine-N,N'-di-2-(3-cyclohexyl)propanoic acid and 1,3-propanediamine-N,N'-di-2-(3-cyclohexyl)propanoic acid derivatives with antiproliferative activity by combining shake flask procedure and UHPLC-MS me. in Pharmazie. 2017;72(6):317-323.
doi:10.1619/ph.2017.6208 .

Tubić, Biljana, Marković, Bojan, Vladimirov, S., Savić, Snežana, Poljarević, Jelena, Sabo, Tibor, "A new model to determine lipophilicity of 1,2-ethanediamine-N,N'-di-2-(3-cyclohexyl)propanoic acid and 1,3-propanediamine-N,N'-di-2-(3-cyclohexyl)propanoic acid derivatives with antiproliferative activity by combining shake flask procedure and UHPLC-MS me" in Pharmazie, 72, no. 6 (2017):317-323,
https://doi.org/10.1619/ph.2017.6208 . .

TY  - JOUR
AU  - Basić, J.
AU  - Kalinić, Marko
AU  - Ivković, Branka
AU  - Erić, Slavica
AU  - Milenković, Marina
AU  - Vladimirov, S.
AU  - Vujić, Zorica
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2114
AB  - Twelve 2'-hydroxy chalcones were synthesized and their in vitro antibacterial activity was tested using eight standard strains of bacteria: S. aureus (ATCC 25923), S. epidermidis (ATCC 12228), B. subtilis (ATCC 6633), M. luteus (ATCC 10240), M. flavus (ATCC 9341), E. faecalis (ATCC 29212), K. pneumoniae (NCIMB 9111) and P. aeruginosa (ATCC 27853). All 2'-hydroxy chalcones have shown moderate to good antimicrobial activity, determined by microdilution method. QSAR analysis was performed for all the cases, R-2 = 0.918 - 0.997. The results of our QSAR analysis indicate that an alternative and complementary mechanism of action is a major determinant of 2'-hydroxy chalcone antibacterial efficiency. These chalcone derivatives posses the ability to act as bidendate chelating agents whereby the ketone moiety forms a coordinate bond and the 2'-hydroxy group forms a covalent bond with a corresponding metal ion. Chelate formation can disrupt the function of bacterial metalloproteins wich may affect the further growth of bacterial cells.
PB  - Inst Materials Physics, Bucharest
T2  - Digest Journal of Nanomaterials and Biostructures
T1  - Synthesis, QSAR analysis and mechanism of antybacterial activity of simple 2 '-hydroxy chalcones
VL  - 9
IS  - 4
SP  - 1537
EP  - 1546
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2114
ER  - 

@article{
author = "Basić, J. and Kalinić, Marko and Ivković, Branka and Erić, Slavica and Milenković, Marina and Vladimirov, S. and Vujić, Zorica",
year = "2014",
abstract = "Twelve 2'-hydroxy chalcones were synthesized and their in vitro antibacterial activity was tested using eight standard strains of bacteria: S. aureus (ATCC 25923), S. epidermidis (ATCC 12228), B. subtilis (ATCC 6633), M. luteus (ATCC 10240), M. flavus (ATCC 9341), E. faecalis (ATCC 29212), K. pneumoniae (NCIMB 9111) and P. aeruginosa (ATCC 27853). All 2'-hydroxy chalcones have shown moderate to good antimicrobial activity, determined by microdilution method. QSAR analysis was performed for all the cases, R-2 = 0.918 - 0.997. The results of our QSAR analysis indicate that an alternative and complementary mechanism of action is a major determinant of 2'-hydroxy chalcone antibacterial efficiency. These chalcone derivatives posses the ability to act as bidendate chelating agents whereby the ketone moiety forms a coordinate bond and the 2'-hydroxy group forms a covalent bond with a corresponding metal ion. Chelate formation can disrupt the function of bacterial metalloproteins wich may affect the further growth of bacterial cells.",
publisher = "Inst Materials Physics, Bucharest",
journal = "Digest Journal of Nanomaterials and Biostructures",
title = "Synthesis, QSAR analysis and mechanism of antybacterial activity of simple 2 '-hydroxy chalcones",
volume = "9",
number = "4",
pages = "1537-1546",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2114"
}

Basić, J., Kalinić, M., Ivković, B., Erić, S., Milenković, M., Vladimirov, S.,& Vujić, Z.. (2014). Synthesis, QSAR analysis and mechanism of antybacterial activity of simple 2 '-hydroxy chalcones. in Digest Journal of Nanomaterials and Biostructures
Inst Materials Physics, Bucharest., 9(4), 1537-1546.
https://hdl.handle.net/21.15107/rcub_farfar_2114

Basić J, Kalinić M, Ivković B, Erić S, Milenković M, Vladimirov S, Vujić Z. Synthesis, QSAR analysis and mechanism of antybacterial activity of simple 2 '-hydroxy chalcones. in Digest Journal of Nanomaterials and Biostructures. 2014;9(4):1537-1546.
https://hdl.handle.net/21.15107/rcub_farfar_2114 .

Basić, J., Kalinić, Marko, Ivković, Branka, Erić, Slavica, Milenković, Marina, Vladimirov, S., Vujić, Zorica, "Synthesis, QSAR analysis and mechanism of antybacterial activity of simple 2 '-hydroxy chalcones" in Digest Journal of Nanomaterials and Biostructures, 9, no. 4 (2014):1537-1546,
https://hdl.handle.net/21.15107/rcub_farfar_2114 .

TY  - JOUR
AU  - Ivković, Branka
AU  - Gojković-Bukarica, Ljiljana
AU  - Vladimirov, S.
AU  - Novaković, Radmila
AU  - Ćupić, Vitomir
AU  - Lešić, A.
AU  - Bumbaširević, M.
AU  - Šćepanović, Radisav
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2063
AB  - The information on the inhibitory effect of propafenone in vascular smooth muscle is sparse. Propafenone acts through blockage of voltage-dependent cardiac Na+ channels, L-type Ca2+ channels, voltage-sensitive K+ (Kv) channels, as well as β-adrenergic receptors in the heart. The introduction of different chemical groups in the benzyl moiety of propafenone influences pharmacological properties of newly developed derivate of propafenone. Here we investigated the effect of new ortho-chloro derivate of propafenone (5OCl) on the vascular tone of precontracted rat aorta. 5OCl produced endothelium-independent relaxation of rat aorta. In order to test the involvement of different ion channels in 5OCl mechanism of action, antagonist of Na+, lidocaine, KV channels, 4-aminopyiridine (4-AP) and L-type Ca2+ channels, nifedipine were used. All tested antagonists of ion channels did not influence the relaxation of rat aorta induced by high a concentration of 5OCl (≥10 μM), but antagonized the relaxation induced by low concentrations of this propafenone derivate. Thus, 5OCl derivate has comparable potency and efficacy as propafenone. According to its interaction with lidocaine, 4-AP and nifedipine it seems that 5OCl partly shares the mechanism of action with propafenone. The mechanism of vasodilatation induced by high micromolar concentration of 5OCl is not defined and further investigations are necessary.
AB  - Informacije o efektima propafenona na vaskularne glatke mišiće su oskudne. Propafenon blokira voltažno-zavisne Na+ kanale, Ca2+ kanale L-tipa, voltažno-senzitivne K+ (Kv) kanale i β-adrenergičke receptore u srcu. Uvođenje različitih hemijskih grupa u benzilni deo molekula propafenona utiče na promenu njegovih farmakoloških osobina. U ovoj studiji je ispitivan uticaj novog orto-hloro derivata (5OCl) propafenona na vaskularni tonus prekontrahovane aorte pacova. Orto hlorni derivat (5OCl) je izazvao endotel-nezavisnu relaksaciju aortnih prstenova. Da bi se ispitala uloga različitih jonskih kanala u ovoj relaksaciji, korišćeni su lidokain, (antagonist Na+ kanala), 4-aminopiridin (antagonist Kv kanala) i nifedipin (antagonist Ca2+ kanala L-tipa). Testirani antagonisti jonskih kanala nisu uticali na relaksaciju aorte pacova izazvanu visokom koncentracijom 5OCl (≥10 μM), ali su zato antagonizovali relaksaciju aorte koncentracijama 5OCl koje su bile manje od 10 μM. Prema tome, 5OCl derivat ima sličnu jačinu i efikasnost kao propafenon. Prema njegovoj interakciji sa lidokainom, 4-AP i nifedipinom može se reći da je mehanizam dejstva 5OCl sličan propafenonu. Mehanizam vazodilatacije 5OCl derivata u koncentracijama većim od 10 μM nije definisan i za to su potrebna dalja istraživanja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - The novel ortho-chloro derivate propafenone induced relaxation in isolated rat aorta
T1  - Relaksacija aorte pacova indukovana novosintetisanim orto-hlornim derivatom propafenona
VL  - 63
IS  - 4
SP  - 363
EP  - 371
DO  - 10.2298/AVB1304363I
ER  - 

@article{
author = "Ivković, Branka and Gojković-Bukarica, Ljiljana and Vladimirov, S. and Novaković, Radmila and Ćupić, Vitomir and Lešić, A. and Bumbaširević, M. and Šćepanović, Radisav",
year = "2013",
abstract = "The information on the inhibitory effect of propafenone in vascular smooth muscle is sparse. Propafenone acts through blockage of voltage-dependent cardiac Na+ channels, L-type Ca2+ channels, voltage-sensitive K+ (Kv) channels, as well as β-adrenergic receptors in the heart. The introduction of different chemical groups in the benzyl moiety of propafenone influences pharmacological properties of newly developed derivate of propafenone. Here we investigated the effect of new ortho-chloro derivate of propafenone (5OCl) on the vascular tone of precontracted rat aorta. 5OCl produced endothelium-independent relaxation of rat aorta. In order to test the involvement of different ion channels in 5OCl mechanism of action, antagonist of Na+, lidocaine, KV channels, 4-aminopyiridine (4-AP) and L-type Ca2+ channels, nifedipine were used. All tested antagonists of ion channels did not influence the relaxation of rat aorta induced by high a concentration of 5OCl (≥10 μM), but antagonized the relaxation induced by low concentrations of this propafenone derivate. Thus, 5OCl derivate has comparable potency and efficacy as propafenone. According to its interaction with lidocaine, 4-AP and nifedipine it seems that 5OCl partly shares the mechanism of action with propafenone. The mechanism of vasodilatation induced by high micromolar concentration of 5OCl is not defined and further investigations are necessary., Informacije o efektima propafenona na vaskularne glatke mišiće su oskudne. Propafenon blokira voltažno-zavisne Na+ kanale, Ca2+ kanale L-tipa, voltažno-senzitivne K+ (Kv) kanale i β-adrenergičke receptore u srcu. Uvođenje različitih hemijskih grupa u benzilni deo molekula propafenona utiče na promenu njegovih farmakoloških osobina. U ovoj studiji je ispitivan uticaj novog orto-hloro derivata (5OCl) propafenona na vaskularni tonus prekontrahovane aorte pacova. Orto hlorni derivat (5OCl) je izazvao endotel-nezavisnu relaksaciju aortnih prstenova. Da bi se ispitala uloga različitih jonskih kanala u ovoj relaksaciji, korišćeni su lidokain, (antagonist Na+ kanala), 4-aminopiridin (antagonist Kv kanala) i nifedipin (antagonist Ca2+ kanala L-tipa). Testirani antagonisti jonskih kanala nisu uticali na relaksaciju aorte pacova izazvanu visokom koncentracijom 5OCl (≥10 μM), ali su zato antagonizovali relaksaciju aorte koncentracijama 5OCl koje su bile manje od 10 μM. Prema tome, 5OCl derivat ima sličnu jačinu i efikasnost kao propafenon. Prema njegovoj interakciji sa lidokainom, 4-AP i nifedipinom može se reći da je mehanizam dejstva 5OCl sličan propafenonu. Mehanizam vazodilatacije 5OCl derivata u koncentracijama većim od 10 μM nije definisan i za to su potrebna dalja istraživanja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "The novel ortho-chloro derivate propafenone induced relaxation in isolated rat aorta, Relaksacija aorte pacova indukovana novosintetisanim orto-hlornim derivatom propafenona",
volume = "63",
number = "4",
pages = "363-371",
doi = "10.2298/AVB1304363I"
}

Ivković, B., Gojković-Bukarica, L., Vladimirov, S., Novaković, R., Ćupić, V., Lešić, A., Bumbaširević, M.,& Šćepanović, R.. (2013). The novel ortho-chloro derivate propafenone induced relaxation in isolated rat aorta. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(4), 363-371.
https://doi.org/10.2298/AVB1304363I

Ivković B, Gojković-Bukarica L, Vladimirov S, Novaković R, Ćupić V, Lešić A, Bumbaširević M, Šćepanović R. The novel ortho-chloro derivate propafenone induced relaxation in isolated rat aorta. in Acta veterinaria. 2013;63(4):363-371.
doi:10.2298/AVB1304363I .

Ivković, Branka, Gojković-Bukarica, Ljiljana, Vladimirov, S., Novaković, Radmila, Ćupić, Vitomir, Lešić, A., Bumbaširević, M., Šćepanović, Radisav, "The novel ortho-chloro derivate propafenone induced relaxation in isolated rat aorta" in Acta veterinaria, 63, no. 4 (2013):363-371,
https://doi.org/10.2298/AVB1304363I . .

TY  - CONF
AU  - Marković, Bojan
AU  - Vladimirov, S.
AU  - Pitić, D.
AU  - Savić, Vladimir
AU  - Jaćević, Vesna
AU  - Dobrić, Silva
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1196
AB  - In this study it was described synthesis of new C-21 esters of fluocinolone acetonide with alpha-alkoxyalcanoic and alpha-aryloxyalkanoic acids. Esterification with these branched oxyacids has to improve benefit-risk ratio. All synthesized compound were shown some topical anti-inflammatory activity.
PB  - Medimond S R L, 40128 Bologna
C3  - Joint Meeting on Medical Chemistry
T1  - Synthesis and Anti-inflammatory Activity of New alpha-Oxyalcanoyl Esters of Fluocinolone Acetonide
SP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1196
ER  - 

@conference{
author = "Marković, Bojan and Vladimirov, S. and Pitić, D. and Savić, Vladimir and Jaćević, Vesna and Dobrić, Silva",
year = "2009",
abstract = "In this study it was described synthesis of new C-21 esters of fluocinolone acetonide with alpha-alkoxyalcanoic and alpha-aryloxyalkanoic acids. Esterification with these branched oxyacids has to improve benefit-risk ratio. All synthesized compound were shown some topical anti-inflammatory activity.",
publisher = "Medimond S R L, 40128 Bologna",
journal = "Joint Meeting on Medical Chemistry",
title = "Synthesis and Anti-inflammatory Activity of New alpha-Oxyalcanoyl Esters of Fluocinolone Acetonide",
pages = "41",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1196"
}

Marković, B., Vladimirov, S., Pitić, D., Savić, V., Jaćević, V.,& Dobrić, S.. (2009). Synthesis and Anti-inflammatory Activity of New alpha-Oxyalcanoyl Esters of Fluocinolone Acetonide. in Joint Meeting on Medical Chemistry
Medimond S R L, 40128 Bologna., 41.
https://hdl.handle.net/21.15107/rcub_farfar_1196

Marković B, Vladimirov S, Pitić D, Savić V, Jaćević V, Dobrić S. Synthesis and Anti-inflammatory Activity of New alpha-Oxyalcanoyl Esters of Fluocinolone Acetonide. in Joint Meeting on Medical Chemistry. 2009;:41.
https://hdl.handle.net/21.15107/rcub_farfar_1196 .

Marković, Bojan, Vladimirov, S., Pitić, D., Savić, Vladimir, Jaćević, Vesna, Dobrić, Silva, "Synthesis and Anti-inflammatory Activity of New alpha-Oxyalcanoyl Esters of Fluocinolone Acetonide" in Joint Meeting on Medical Chemistry (2009):41,
https://hdl.handle.net/21.15107/rcub_farfar_1196 .

TY  - JOUR
AU  - Tubić, Biljana
AU  - Ivković, Branka
AU  - Zečević, Mira
AU  - Vladimirov, S.
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/992
AB  - A simple, rapid and reproducible reversed-phase high-performance liquid chromatography method for the analysis of nimesulide and its impurities both in the bulk drug and pharmaceutical formulations is reported. The method is suitable for monitoring the stability of nimesulide. The presence of nimesulide impurities C (2- phenoxyaniline) and D (2- phenoxy4- nitroaniline) was observed. The best separation was achieved using an Agilent Zorbax Extend C-18 column ( 150 x 4.6 mm, particle size 5 mu m) at 40 C and flow rate of 1.0 mLmin(-1). The analytes were monitored at 230 nm. The mobile phase consisted of acetonitrile - triethylamine (TEA) - water (45:0.5:54.5 v/v/v), adjusted to pH 5.2 with formic acid. Under these conditions the retention times were of 7.11, 7.98 and 8.66 min for nimesulide, D and C, respectively. The resolution of nimesulide and impurity D was 3.20 and that of impurity D and impurity C 2.40, indicating that the compounds were well separated. Evaluation of linearity, accuracy, precision, selectivity, sensitivity and robustness of the method produced satisfactory results. The developed method was successfully applied to assay nimesulide in different solid pharmaceutical formulations.
PB  - Slovensko Kemijsko Drustvo, Ljubljana
T2  - Acta Chimica Slovenica
T1  - Simulataneous determination of nimesulide and its impurities in pharmaceutical formulations by reversed-phase high-performance liquid chromatography
VL  - 54
IS  - 3
SP  - 583
EP  - 590
UR  - https://hdl.handle.net/21.15107/rcub_farfar_992
ER  - 

@article{
author = "Tubić, Biljana and Ivković, Branka and Zečević, Mira and Vladimirov, S.",
year = "2007",
abstract = "A simple, rapid and reproducible reversed-phase high-performance liquid chromatography method for the analysis of nimesulide and its impurities both in the bulk drug and pharmaceutical formulations is reported. The method is suitable for monitoring the stability of nimesulide. The presence of nimesulide impurities C (2- phenoxyaniline) and D (2- phenoxy4- nitroaniline) was observed. The best separation was achieved using an Agilent Zorbax Extend C-18 column ( 150 x 4.6 mm, particle size 5 mu m) at 40 C and flow rate of 1.0 mLmin(-1). The analytes were monitored at 230 nm. The mobile phase consisted of acetonitrile - triethylamine (TEA) - water (45:0.5:54.5 v/v/v), adjusted to pH 5.2 with formic acid. Under these conditions the retention times were of 7.11, 7.98 and 8.66 min for nimesulide, D and C, respectively. The resolution of nimesulide and impurity D was 3.20 and that of impurity D and impurity C 2.40, indicating that the compounds were well separated. Evaluation of linearity, accuracy, precision, selectivity, sensitivity and robustness of the method produced satisfactory results. The developed method was successfully applied to assay nimesulide in different solid pharmaceutical formulations.",
publisher = "Slovensko Kemijsko Drustvo, Ljubljana",
journal = "Acta Chimica Slovenica",
title = "Simulataneous determination of nimesulide and its impurities in pharmaceutical formulations by reversed-phase high-performance liquid chromatography",
volume = "54",
number = "3",
pages = "583-590",
url = "https://hdl.handle.net/21.15107/rcub_farfar_992"
}

Tubić, B., Ivković, B., Zečević, M.,& Vladimirov, S.. (2007). Simulataneous determination of nimesulide and its impurities in pharmaceutical formulations by reversed-phase high-performance liquid chromatography. in Acta Chimica Slovenica
Slovensko Kemijsko Drustvo, Ljubljana., 54(3), 583-590.
https://hdl.handle.net/21.15107/rcub_farfar_992

Tubić B, Ivković B, Zečević M, Vladimirov S. Simulataneous determination of nimesulide and its impurities in pharmaceutical formulations by reversed-phase high-performance liquid chromatography. in Acta Chimica Slovenica. 2007;54(3):583-590.
https://hdl.handle.net/21.15107/rcub_farfar_992 .

Tubić, Biljana, Ivković, Branka, Zečević, Mira, Vladimirov, S., "Simulataneous determination of nimesulide and its impurities in pharmaceutical formulations by reversed-phase high-performance liquid chromatography" in Acta Chimica Slovenica, 54, no. 3 (2007):583-590,
https://hdl.handle.net/21.15107/rcub_farfar_992 .

TY  - CONF
AU  - Brborić, Jasmina
AU  - Jovanović, M.
AU  - Ivković, Branka
AU  - Vladimirov, S.
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/749
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - RP-HPLC metoda za određivanje hemijske čistoće analoga iminodisirćetne kiseline-liganada u 99mTC-radiofarmačeutičima za hepatobilijarnu scintigrafiju
T1  - RP-HPLC method for the determination of purity of iminodiacetic acid analogs-ligands in 99mTC-radiopharmaceuticals for hepatobiliary scintigraphy
VL  - 56
IS  - 5
SP  - 730
EP  - 731
UR  - https://hdl.handle.net/21.15107/rcub_farfar_749
ER  - 

@conference{
author = "Brborić, Jasmina and Jovanović, M. and Ivković, Branka and Vladimirov, S.",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "RP-HPLC metoda za određivanje hemijske čistoće analoga iminodisirćetne kiseline-liganada u 99mTC-radiofarmačeutičima za hepatobilijarnu scintigrafiju, RP-HPLC method for the determination of purity of iminodiacetic acid analogs-ligands in 99mTC-radiopharmaceuticals for hepatobiliary scintigraphy",
volume = "56",
number = "5",
pages = "730-731",
url = "https://hdl.handle.net/21.15107/rcub_farfar_749"
}

Brborić, J., Jovanović, M., Ivković, B.,& Vladimirov, S.. (2006). RP-HPLC metoda za određivanje hemijske čistoće analoga iminodisirćetne kiseline-liganada u 99mTC-radiofarmačeutičima za hepatobilijarnu scintigrafiju. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(5), 730-731.
https://hdl.handle.net/21.15107/rcub_farfar_749

Brborić J, Jovanović M, Ivković B, Vladimirov S. RP-HPLC metoda za određivanje hemijske čistoće analoga iminodisirćetne kiseline-liganada u 99mTC-radiofarmačeutičima za hepatobilijarnu scintigrafiju. in Arhiv za farmaciju. 2006;56(5):730-731.
https://hdl.handle.net/21.15107/rcub_farfar_749 .

Brborić, Jasmina, Jovanović, M., Ivković, Branka, Vladimirov, S., "RP-HPLC metoda za određivanje hemijske čistoće analoga iminodisirćetne kiseline-liganada u 99mTC-radiofarmačeutičima za hepatobilijarnu scintigrafiju" in Arhiv za farmaciju, 56, no. 5 (2006):730-731,
https://hdl.handle.net/21.15107/rcub_farfar_749 .

TY  - CONF
AU  - Čudina, Olivera
AU  - Janković, I.
AU  - Karljiković-Rajić, Katarina
AU  - Vladimirov, S.
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/720
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - An interaction study of valsartan/cationic surfactant using absorption UV spectrophotometry
T1  - Ispitivanje interakcija valsartan/katjonski surfaktant primenom apsorpcione UV spektrofotometrije
VL  - 56
IS  - 5
SP  - 690
EP  - 691
UR  - https://hdl.handle.net/21.15107/rcub_farfar_720
ER  - 

@conference{
author = "Čudina, Olivera and Janković, I. and Karljiković-Rajić, Katarina and Vladimirov, S.",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "An interaction study of valsartan/cationic surfactant using absorption UV spectrophotometry, Ispitivanje interakcija valsartan/katjonski surfaktant primenom apsorpcione UV spektrofotometrije",
volume = "56",
number = "5",
pages = "690-691",
url = "https://hdl.handle.net/21.15107/rcub_farfar_720"
}

Čudina, O., Janković, I., Karljiković-Rajić, K.,& Vladimirov, S.. (2006). An interaction study of valsartan/cationic surfactant using absorption UV spectrophotometry. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(5), 690-691.
https://hdl.handle.net/21.15107/rcub_farfar_720

Čudina O, Janković I, Karljiković-Rajić K, Vladimirov S. An interaction study of valsartan/cationic surfactant using absorption UV spectrophotometry. in Arhiv za farmaciju. 2006;56(5):690-691.
https://hdl.handle.net/21.15107/rcub_farfar_720 .

Čudina, Olivera, Janković, I., Karljiković-Rajić, Katarina, Vladimirov, S., "An interaction study of valsartan/cationic surfactant using absorption UV spectrophotometry" in Arhiv za farmaciju, 56, no. 5 (2006):690-691,
https://hdl.handle.net/21.15107/rcub_farfar_720 .

TY  - CONF
AU  - Pešut, L.
AU  - Marković, Bojan
AU  - Vladimirov, S.
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/751
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - High performance liquid chromatography assay of itraconazole in dosage forms
T1  - HPLC određivanje sadržaja itrakonazola iz doziranih oblika
VL  - 56
IS  - 5
SP  - 750
EP  - 751
UR  - https://hdl.handle.net/21.15107/rcub_farfar_751
ER  - 

@conference{
author = "Pešut, L. and Marković, Bojan and Vladimirov, S.",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "High performance liquid chromatography assay of itraconazole in dosage forms, HPLC određivanje sadržaja itrakonazola iz doziranih oblika",
volume = "56",
number = "5",
pages = "750-751",
url = "https://hdl.handle.net/21.15107/rcub_farfar_751"
}

Pešut, L., Marković, B.,& Vladimirov, S.. (2006). High performance liquid chromatography assay of itraconazole in dosage forms. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(5), 750-751.
https://hdl.handle.net/21.15107/rcub_farfar_751

Pešut L, Marković B, Vladimirov S. High performance liquid chromatography assay of itraconazole in dosage forms. in Arhiv za farmaciju. 2006;56(5):750-751.
https://hdl.handle.net/21.15107/rcub_farfar_751 .

Pešut, L., Marković, Bojan, Vladimirov, S., "High performance liquid chromatography assay of itraconazole in dosage forms" in Arhiv za farmaciju, 56, no. 5 (2006):750-751,
https://hdl.handle.net/21.15107/rcub_farfar_751 .

TY  - CONF
AU  - Kljajić, N.
AU  - Ivković, Branka
AU  - Vladimirov, S.
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/667
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Simultaneous determination of active substances in Influrex® dosage form using rapid reverse-phase high performance liquid chromatography
T1  - Određivanje sadržaja aktivnih supstanci u doziranom obliku Influrex® primenom visokoefikasne tečne hromatografije
VL  - 56
IS  - 5
SP  - 746
EP  - 747
UR  - https://hdl.handle.net/21.15107/rcub_farfar_667
ER  - 

@conference{
author = "Kljajić, N. and Ivković, Branka and Vladimirov, S.",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Simultaneous determination of active substances in Influrex® dosage form using rapid reverse-phase high performance liquid chromatography, Određivanje sadržaja aktivnih supstanci u doziranom obliku Influrex® primenom visokoefikasne tečne hromatografije",
volume = "56",
number = "5",
pages = "746-747",
url = "https://hdl.handle.net/21.15107/rcub_farfar_667"
}

Kljajić, N., Ivković, B.,& Vladimirov, S.. (2006). Simultaneous determination of active substances in Influrex® dosage form using rapid reverse-phase high performance liquid chromatography. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(5), 746-747.
https://hdl.handle.net/21.15107/rcub_farfar_667

Kljajić N, Ivković B, Vladimirov S. Simultaneous determination of active substances in Influrex® dosage form using rapid reverse-phase high performance liquid chromatography. in Arhiv za farmaciju. 2006;56(5):746-747.
https://hdl.handle.net/21.15107/rcub_farfar_667 .

Kljajić, N., Ivković, Branka, Vladimirov, S., "Simultaneous determination of active substances in Influrex® dosage form using rapid reverse-phase high performance liquid chromatography" in Arhiv za farmaciju, 56, no. 5 (2006):746-747,
https://hdl.handle.net/21.15107/rcub_farfar_667 .

TY  - CONF
AU  - Crevar, Milkica
AU  - Ivković, Branka
AU  - Vladimirov, S.
AU  - Vujić, Zorica
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/678
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Statistical optimization of reverse phase high performance liquid chromatography for the analysis of caffeine, paracetamole and its degradation product P-aminophenole
T1  - Statistička optimizačija reverzno fazne visokoefikasne tečne hromatografije za analizu kofeina, paracetamola i njegovog degradacionog proizvoda P-aminofenola
VL  - 56
IS  - 5
SP  - 734
EP  - 735
UR  - https://hdl.handle.net/21.15107/rcub_farfar_678
ER  - 

@conference{
author = "Crevar, Milkica and Ivković, Branka and Vladimirov, S. and Vujić, Zorica",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Statistical optimization of reverse phase high performance liquid chromatography for the analysis of caffeine, paracetamole and its degradation product P-aminophenole, Statistička optimizačija reverzno fazne visokoefikasne tečne hromatografije za analizu kofeina, paracetamola i njegovog degradacionog proizvoda P-aminofenola",
volume = "56",
number = "5",
pages = "734-735",
url = "https://hdl.handle.net/21.15107/rcub_farfar_678"
}

Crevar, M., Ivković, B., Vladimirov, S.,& Vujić, Z.. (2006). Statistical optimization of reverse phase high performance liquid chromatography for the analysis of caffeine, paracetamole and its degradation product P-aminophenole. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(5), 734-735.
https://hdl.handle.net/21.15107/rcub_farfar_678

Crevar M, Ivković B, Vladimirov S, Vujić Z. Statistical optimization of reverse phase high performance liquid chromatography for the analysis of caffeine, paracetamole and its degradation product P-aminophenole. in Arhiv za farmaciju. 2006;56(5):734-735.
https://hdl.handle.net/21.15107/rcub_farfar_678 .

Crevar, Milkica, Ivković, Branka, Vladimirov, S., Vujić, Zorica, "Statistical optimization of reverse phase high performance liquid chromatography for the analysis of caffeine, paracetamole and its degradation product P-aminophenole" in Arhiv za farmaciju, 56, no. 5 (2006):734-735,
https://hdl.handle.net/21.15107/rcub_farfar_678 .