TY  - JOUR
AU  - Đorović, Đorđe
AU  - Lazarević, Vesna
AU  - Aranđelović, Jovana
AU  - Stevanović, Vladimir
AU  - Paslawski, Wojciech
AU  - Zhang, Xiaoqun
AU  - Velimirović, Milica
AU  - Petronijević, Nataša
AU  - Puškaš, Laslo
AU  - Savić, Miroslav
AU  - Svenningsson, Per
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5503
AB  - Background: Early life stress is a major risk factor for later development of psychiatric disorders, including post-traumatic stress disorder (PTSD). An intricate relationship exists between various neurotransmitters (such as glutamate, norepinephrine or serotonin), calcium/calmodulin-dependent protein kinase II (CaMKII), as an important regulator of glutamatergic synaptic function, and PTSD. Here, we developed a double-hit model to investigate the interaction of maternal deprivation (MD) as an early life stress model and single prolonged stress (SPS) as a PTSD model at the behavioral and molecular levels. Methods: Male Wistar rats exposed to these stress paradigms were subjected to a comprehensive behavioral analysis. In hippocampal synaptosomes we investigated neurotransmitter release and glutamate concentration. The expression of CaMKII and the content of monoamines were determined in selected brain regions. Brain-derived neurotrophic factor (BDNF) mRNA was quantified by radioactive in situ hybridization. Results: We report a distinct behavioral phenotype in the double-hit group. Double-hit and SPS groups had decreased hippocampal presynaptic glutamatergic function. In hippocampus, double-hit stress caused a decrease in autophosphorylation of CaMKII. In prefrontal cortex, both SPS and double-hit stress had a similar effect on CaMKII autophosphorylation. Double-hit stress, rather than SPS, affected the norepinephrine and serotonin levels in prefrontal cortex, and suppressed BDNF gene expression in prefrontal cortex and hippocampus. Limitations: The study was conducted in male rats only. The affected brain regions cannot be restricted to hippocampus, prefrontal cortex and amygdala. Conclusion: Double-hit stress caused more pronounced and distinct behavioral, molecular and functional changes, compared to MD or SPS alone.
PB  - Elsevier B.V.
T2  - Journal of Affective Disorders
T1  - Maternal deprivation causes CaMKII downregulation and modulates glutamate, norepinephrine and serotonin in limbic brain areas in a rat model of single prolonged stress
VL  - 349
SP  - 286
EP  - 296
DO  - 10.1016/j.jad.2024.01.087
ER  - 

@article{
author = "Đorović, Đorđe and Lazarević, Vesna and Aranđelović, Jovana and Stevanović, Vladimir and Paslawski, Wojciech and Zhang, Xiaoqun and Velimirović, Milica and Petronijević, Nataša and Puškaš, Laslo and Savić, Miroslav and Svenningsson, Per",
year = "2024",
abstract = "Background: Early life stress is a major risk factor for later development of psychiatric disorders, including post-traumatic stress disorder (PTSD). An intricate relationship exists between various neurotransmitters (such as glutamate, norepinephrine or serotonin), calcium/calmodulin-dependent protein kinase II (CaMKII), as an important regulator of glutamatergic synaptic function, and PTSD. Here, we developed a double-hit model to investigate the interaction of maternal deprivation (MD) as an early life stress model and single prolonged stress (SPS) as a PTSD model at the behavioral and molecular levels. Methods: Male Wistar rats exposed to these stress paradigms were subjected to a comprehensive behavioral analysis. In hippocampal synaptosomes we investigated neurotransmitter release and glutamate concentration. The expression of CaMKII and the content of monoamines were determined in selected brain regions. Brain-derived neurotrophic factor (BDNF) mRNA was quantified by radioactive in situ hybridization. Results: We report a distinct behavioral phenotype in the double-hit group. Double-hit and SPS groups had decreased hippocampal presynaptic glutamatergic function. In hippocampus, double-hit stress caused a decrease in autophosphorylation of CaMKII. In prefrontal cortex, both SPS and double-hit stress had a similar effect on CaMKII autophosphorylation. Double-hit stress, rather than SPS, affected the norepinephrine and serotonin levels in prefrontal cortex, and suppressed BDNF gene expression in prefrontal cortex and hippocampus. Limitations: The study was conducted in male rats only. The affected brain regions cannot be restricted to hippocampus, prefrontal cortex and amygdala. Conclusion: Double-hit stress caused more pronounced and distinct behavioral, molecular and functional changes, compared to MD or SPS alone.",
publisher = "Elsevier B.V.",
journal = "Journal of Affective Disorders",
title = "Maternal deprivation causes CaMKII downregulation and modulates glutamate, norepinephrine and serotonin in limbic brain areas in a rat model of single prolonged stress",
volume = "349",
pages = "286-296",
doi = "10.1016/j.jad.2024.01.087"
}

Đorović, Đ., Lazarević, V., Aranđelović, J., Stevanović, V., Paslawski, W., Zhang, X., Velimirović, M., Petronijević, N., Puškaš, L., Savić, M.,& Svenningsson, P.. (2024). Maternal deprivation causes CaMKII downregulation and modulates glutamate, norepinephrine and serotonin in limbic brain areas in a rat model of single prolonged stress. in Journal of Affective Disorders
Elsevier B.V.., 349, 286-296.
https://doi.org/10.1016/j.jad.2024.01.087

Đorović Đ, Lazarević V, Aranđelović J, Stevanović V, Paslawski W, Zhang X, Velimirović M, Petronijević N, Puškaš L, Savić M, Svenningsson P. Maternal deprivation causes CaMKII downregulation and modulates glutamate, norepinephrine and serotonin in limbic brain areas in a rat model of single prolonged stress. in Journal of Affective Disorders. 2024;349:286-296.
doi:10.1016/j.jad.2024.01.087 .

Đorović, Đorđe, Lazarević, Vesna, Aranđelović, Jovana, Stevanović, Vladimir, Paslawski, Wojciech, Zhang, Xiaoqun, Velimirović, Milica, Petronijević, Nataša, Puškaš, Laslo, Savić, Miroslav, Svenningsson, Per, "Maternal deprivation causes CaMKII downregulation and modulates glutamate, norepinephrine and serotonin in limbic brain areas in a rat model of single prolonged stress" in Journal of Affective Disorders, 349 (2024):286-296,
https://doi.org/10.1016/j.jad.2024.01.087 . .

TY  - JOUR
AU  - Savić, Snežana
AU  - Savić, M.
AU  - Tamburić, Slobodanka
AU  - Vuleta, G.
AU  - Vesić, Sonja
AU  - Mueller-Goymann, Christel
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/939
AB  - There is a growing need for research into new skin- and environment-friendly surfactants. This paper focuses on a natural surfactant of an alkylpolyglucoside type, which can form both thermotropic and lyotropic liquid-crystalline phases. The aim of this study was to relate some physicochemical properties (characterised by polarisation and transmission electron microscopy, thermal analysis and rheology) of the three formulations based on cetearyl glucoside and cetearyl alcohol, to the results of in vitro and in vivo bioavailability of hydrocortisone (HC). The three formulations contained oils of different polarity (medium chain triglycerides: MG, isopropyl myristate: IPM and light liquid paraffin: LP), respectively In vitro permeation was followed through the artificial skin constructs (ASC), while the parameters measured in vivo were erythema index: EI (using instrumental human skin blanching assay), transepidermal water loss (TEWL) and stratum corneum. hydration (SCH). The vehicles based on cetearyl glucoside and cetearyl alcohol showed a complex colloidal structure of lamellar liquid-crystalline and lamellar gel-crystalline type, depending on oil polarity. Rheological profile of the vehicle was directly related to the in vitro profile of the HC permeation. In vivo results suggested that the vehicle with MG retarded the HC permeation, whereas less polar IPM and non-polar LP enhanced it. It is suggested that the enhancement is achieved either by a direct interaction with lipid lamellae of the SC or indirectly by improving skin hydration. There were no adverse effects during in vivo study, which indicates a good safety profile of this alkylpolyglucoside surfactant.
PB  - Elsevier Science BV, Amsterdam
T2  - European Journal of Pharmaceutical Sciences
T1  - An alkylpolyglucoside surfactant as a prospective pharmaceutical excipient for topical formulations: The influence of oil polarity on the colloidal structure and hydrocortisone in vitro/in vivo permeation
VL  - 30
IS  - 5
SP  - 441
EP  - 450
DO  - 10.1016/j.ejps.2007.01.006
ER  - 

@article{
author = "Savić, Snežana and Savić, M. and Tamburić, Slobodanka and Vuleta, G. and Vesić, Sonja and Mueller-Goymann, Christel",
year = "2007",
abstract = "There is a growing need for research into new skin- and environment-friendly surfactants. This paper focuses on a natural surfactant of an alkylpolyglucoside type, which can form both thermotropic and lyotropic liquid-crystalline phases. The aim of this study was to relate some physicochemical properties (characterised by polarisation and transmission electron microscopy, thermal analysis and rheology) of the three formulations based on cetearyl glucoside and cetearyl alcohol, to the results of in vitro and in vivo bioavailability of hydrocortisone (HC). The three formulations contained oils of different polarity (medium chain triglycerides: MG, isopropyl myristate: IPM and light liquid paraffin: LP), respectively In vitro permeation was followed through the artificial skin constructs (ASC), while the parameters measured in vivo were erythema index: EI (using instrumental human skin blanching assay), transepidermal water loss (TEWL) and stratum corneum. hydration (SCH). The vehicles based on cetearyl glucoside and cetearyl alcohol showed a complex colloidal structure of lamellar liquid-crystalline and lamellar gel-crystalline type, depending on oil polarity. Rheological profile of the vehicle was directly related to the in vitro profile of the HC permeation. In vivo results suggested that the vehicle with MG retarded the HC permeation, whereas less polar IPM and non-polar LP enhanced it. It is suggested that the enhancement is achieved either by a direct interaction with lipid lamellae of the SC or indirectly by improving skin hydration. There were no adverse effects during in vivo study, which indicates a good safety profile of this alkylpolyglucoside surfactant.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "European Journal of Pharmaceutical Sciences",
title = "An alkylpolyglucoside surfactant as a prospective pharmaceutical excipient for topical formulations: The influence of oil polarity on the colloidal structure and hydrocortisone in vitro/in vivo permeation",
volume = "30",
number = "5",
pages = "441-450",
doi = "10.1016/j.ejps.2007.01.006"
}

Savić, S., Savić, M., Tamburić, S., Vuleta, G., Vesić, S.,& Mueller-Goymann, C.. (2007). An alkylpolyglucoside surfactant as a prospective pharmaceutical excipient for topical formulations: The influence of oil polarity on the colloidal structure and hydrocortisone in vitro/in vivo permeation. in European Journal of Pharmaceutical Sciences
Elsevier Science BV, Amsterdam., 30(5), 441-450.
https://doi.org/10.1016/j.ejps.2007.01.006

Savić S, Savić M, Tamburić S, Vuleta G, Vesić S, Mueller-Goymann C. An alkylpolyglucoside surfactant as a prospective pharmaceutical excipient for topical formulations: The influence of oil polarity on the colloidal structure and hydrocortisone in vitro/in vivo permeation. in European Journal of Pharmaceutical Sciences. 2007;30(5):441-450.
doi:10.1016/j.ejps.2007.01.006 .

Savić, Snežana, Savić, M., Tamburić, Slobodanka, Vuleta, G., Vesić, Sonja, Mueller-Goymann, Christel, "An alkylpolyglucoside surfactant as a prospective pharmaceutical excipient for topical formulations: The influence of oil polarity on the colloidal structure and hydrocortisone in vitro/in vivo permeation" in European Journal of Pharmaceutical Sciences, 30, no. 5 (2007):441-450,
https://doi.org/10.1016/j.ejps.2007.01.006 . .

TY  - CONF
AU  - Milošević, A.
AU  - Veljković, M.
AU  - Bogavac-Stanojević, Nataša
AU  - Memon, Lidija
AU  - Jelić-Ivanović, Zorana
AU  - Spasojević-Kalimanovska, Vesna
AU  - Gulan, B.
AU  - Savić, M.
AU  - Todorović, R.
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/732
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - C-reactive protein and in-hospital mortality in acute ischaemic stroke
T1  - C-reaktivni protein i smrtnost kod hospitalizovanih pacijenata sa akutnim ishemijskim moždanim udarom
VL  - 56
IS  - 5
SP  - 664
EP  - 665
UR  - https://hdl.handle.net/21.15107/rcub_farfar_732
ER  - 

@conference{
author = "Milošević, A. and Veljković, M. and Bogavac-Stanojević, Nataša and Memon, Lidija and Jelić-Ivanović, Zorana and Spasojević-Kalimanovska, Vesna and Gulan, B. and Savić, M. and Todorović, R.",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "C-reactive protein and in-hospital mortality in acute ischaemic stroke, C-reaktivni protein i smrtnost kod hospitalizovanih pacijenata sa akutnim ishemijskim moždanim udarom",
volume = "56",
number = "5",
pages = "664-665",
url = "https://hdl.handle.net/21.15107/rcub_farfar_732"
}

Milošević, A., Veljković, M., Bogavac-Stanojević, N., Memon, L., Jelić-Ivanović, Z., Spasojević-Kalimanovska, V., Gulan, B., Savić, M.,& Todorović, R.. (2006). C-reactive protein and in-hospital mortality in acute ischaemic stroke. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(5), 664-665.
https://hdl.handle.net/21.15107/rcub_farfar_732

Milošević A, Veljković M, Bogavac-Stanojević N, Memon L, Jelić-Ivanović Z, Spasojević-Kalimanovska V, Gulan B, Savić M, Todorović R. C-reactive protein and in-hospital mortality in acute ischaemic stroke. in Arhiv za farmaciju. 2006;56(5):664-665.
https://hdl.handle.net/21.15107/rcub_farfar_732 .

Milošević, A., Veljković, M., Bogavac-Stanojević, Nataša, Memon, Lidija, Jelić-Ivanović, Zorana, Spasojević-Kalimanovska, Vesna, Gulan, B., Savić, M., Todorović, R., "C-reactive protein and in-hospital mortality in acute ischaemic stroke" in Arhiv za farmaciju, 56, no. 5 (2006):664-665,
https://hdl.handle.net/21.15107/rcub_farfar_732 .