TY  - JOUR
AU  - Milinković, Neda
AU  - Ignjatović, Svetlana
AU  - Šumarac, Zorica
AU  - Majkić-Singh, Nada
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3210
AB  - An adequate assessment of the measurement uncertainty in a laboratory medicine is one of the most important factors for a reliable interpretation of the results. A large number of standards and guidelines indicate the need for a proper assessment of the uncertainty of measurement results in routine laboratory practice. The available documents gene rally recommend participation in the proficiency schemes/external quality control, as well as the internal quality control, in order to primarily verify the quality performance of the method. Although all documents meet the requirements of the International Standard, ISO 15189, the standard itself does not clearly define the method by which the measurement results need to be assessed and there is no harmonization in practice regarding to this. Also, the uncertainty of measurement results is the data relating to the measured result itself, but all factors that influence the interpretation of the measured value, which is ultimately used for diagnosis and monitoring of the patient's treatment, should be taken into account. So in laboratory medicine, an appropriate assessment of the uncertainty of the measurement results should have the ultimate goal of reducing diagnostic uncertainty. However, good professional laboratory practice and understanding analytical aspects of the test for each individual laboratory is necessary to adequately define the uncertainty of measurement results for specific laboratory tests, which helps to implement good clinical practice. Also, setting diagnoses in medicine is a decision with a certain degree of uncertainty, rather than statistically and mathematically calculated conclusion.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Uncertainty of Measurement in Laboratory Medicine
VL  - 37
IS  - 3
SP  - 279
EP  - 288
DO  - 10.2478/jomb-2018-0002
ER  - 

@article{
author = "Milinković, Neda and Ignjatović, Svetlana and Šumarac, Zorica and Majkić-Singh, Nada",
year = "2018",
abstract = "An adequate assessment of the measurement uncertainty in a laboratory medicine is one of the most important factors for a reliable interpretation of the results. A large number of standards and guidelines indicate the need for a proper assessment of the uncertainty of measurement results in routine laboratory practice. The available documents gene rally recommend participation in the proficiency schemes/external quality control, as well as the internal quality control, in order to primarily verify the quality performance of the method. Although all documents meet the requirements of the International Standard, ISO 15189, the standard itself does not clearly define the method by which the measurement results need to be assessed and there is no harmonization in practice regarding to this. Also, the uncertainty of measurement results is the data relating to the measured result itself, but all factors that influence the interpretation of the measured value, which is ultimately used for diagnosis and monitoring of the patient's treatment, should be taken into account. So in laboratory medicine, an appropriate assessment of the uncertainty of the measurement results should have the ultimate goal of reducing diagnostic uncertainty. However, good professional laboratory practice and understanding analytical aspects of the test for each individual laboratory is necessary to adequately define the uncertainty of measurement results for specific laboratory tests, which helps to implement good clinical practice. Also, setting diagnoses in medicine is a decision with a certain degree of uncertainty, rather than statistically and mathematically calculated conclusion.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Uncertainty of Measurement in Laboratory Medicine",
volume = "37",
number = "3",
pages = "279-288",
doi = "10.2478/jomb-2018-0002"
}

Milinković, N., Ignjatović, S., Šumarac, Z.,& Majkić-Singh, N.. (2018). Uncertainty of Measurement in Laboratory Medicine. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 37(3), 279-288.
https://doi.org/10.2478/jomb-2018-0002

Milinković N, Ignjatović S, Šumarac Z, Majkić-Singh N. Uncertainty of Measurement in Laboratory Medicine. in Journal of Medical Biochemistry. 2018;37(3):279-288.
doi:10.2478/jomb-2018-0002 .

Milinković, Neda, Ignjatović, Svetlana, Šumarac, Zorica, Majkić-Singh, Nada, "Uncertainty of Measurement in Laboratory Medicine" in Journal of Medical Biochemistry, 37, no. 3 (2018):279-288,
https://doi.org/10.2478/jomb-2018-0002 . .

TY  - JOUR
AU  - Krnjeta, Tijana
AU  - Mirković, Ljiljana
AU  - Ignjatović, Svetlana
AU  - Tomasević, Dragana
AU  - Lukić, Jelena
AU  - Topalov, Drina
AU  - Majkić-Singh, Nada
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2848
AB  - Background/Aim. Preeclampsia (PE) belongs to the group of hypertensive disorders in pregnancy with the global average incidence of 2.16%. It is considered as one of the leading causes of maternal and neonatal morbidity and mortality worldwide. The goal of this study was to assess the potential association between Val158Met catechol-o-methyltransferase (COMT), tumor necrosis factor-alpha (TNF-alpha) -857 C>T, tumor necrosis factor receptor 1 (TNFR1) 36 A>G, interleukin-1alpha (IL-1 alpha) 4845 G>T and interleukin-10 (IL-10) -1082 A>G polymorphisms and risk of early-onset preeclampsia (PE) and its complications. Methods. The study included 47 early-onset PE patients, which were grouped by disease severity and by size for gestational age and 47 control cases. The Val158Met polymorphism was genotyped by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) analysis and inflammatory cytokine polymorphisms by the Sanger sequencing method. Results. The COMT Met allele as well as IL-1 alpha T showed a protective role, decreasing the risk of early-onset PE after age and body mass index (BMI) adjustments. The detected interactions between the COMT Met and IL-10 A alleles, as well as between the COMT Met and TNF-alpha T alleles were insignificant after age and BMI adjustments. Conclusion. COMT and IL-1 alpha may be used as candidate genes for early-onset PE and its severe form and small for gestational age (SGA) complications.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Association between Val158Met COMT, TNF-alpha-857 C > T, TNFR1 36 A > G, IL-1 alpha 4845 G > T and IL-10-1082 A > G Polymorphisms and Risk of Early-Onset Preeclampsia and Its Complications
VL  - 74
IS  - 12
SP  - 1155
EP  - 1161
DO  - 10.2298/VSP160329313K
ER  - 

@article{
author = "Krnjeta, Tijana and Mirković, Ljiljana and Ignjatović, Svetlana and Tomasević, Dragana and Lukić, Jelena and Topalov, Drina and Majkić-Singh, Nada",
year = "2017",
abstract = "Background/Aim. Preeclampsia (PE) belongs to the group of hypertensive disorders in pregnancy with the global average incidence of 2.16%. It is considered as one of the leading causes of maternal and neonatal morbidity and mortality worldwide. The goal of this study was to assess the potential association between Val158Met catechol-o-methyltransferase (COMT), tumor necrosis factor-alpha (TNF-alpha) -857 C>T, tumor necrosis factor receptor 1 (TNFR1) 36 A>G, interleukin-1alpha (IL-1 alpha) 4845 G>T and interleukin-10 (IL-10) -1082 A>G polymorphisms and risk of early-onset preeclampsia (PE) and its complications. Methods. The study included 47 early-onset PE patients, which were grouped by disease severity and by size for gestational age and 47 control cases. The Val158Met polymorphism was genotyped by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) analysis and inflammatory cytokine polymorphisms by the Sanger sequencing method. Results. The COMT Met allele as well as IL-1 alpha T showed a protective role, decreasing the risk of early-onset PE after age and body mass index (BMI) adjustments. The detected interactions between the COMT Met and IL-10 A alleles, as well as between the COMT Met and TNF-alpha T alleles were insignificant after age and BMI adjustments. Conclusion. COMT and IL-1 alpha may be used as candidate genes for early-onset PE and its severe form and small for gestational age (SGA) complications.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Association between Val158Met COMT, TNF-alpha-857 C > T, TNFR1 36 A > G, IL-1 alpha 4845 G > T and IL-10-1082 A > G Polymorphisms and Risk of Early-Onset Preeclampsia and Its Complications",
volume = "74",
number = "12",
pages = "1155-1161",
doi = "10.2298/VSP160329313K"
}

Krnjeta, T., Mirković, L., Ignjatović, S., Tomasević, D., Lukić, J., Topalov, D.,& Majkić-Singh, N.. (2017). Association between Val158Met COMT, TNF-alpha-857 C > T, TNFR1 36 A > G, IL-1 alpha 4845 G > T and IL-10-1082 A > G Polymorphisms and Risk of Early-Onset Preeclampsia and Its Complications. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 74(12), 1155-1161.
https://doi.org/10.2298/VSP160329313K

Krnjeta T, Mirković L, Ignjatović S, Tomasević D, Lukić J, Topalov D, Majkić-Singh N. Association between Val158Met COMT, TNF-alpha-857 C > T, TNFR1 36 A > G, IL-1 alpha 4845 G > T and IL-10-1082 A > G Polymorphisms and Risk of Early-Onset Preeclampsia and Its Complications. in Vojnosanitetski pregled. 2017;74(12):1155-1161.
doi:10.2298/VSP160329313K .

Krnjeta, Tijana, Mirković, Ljiljana, Ignjatović, Svetlana, Tomasević, Dragana, Lukić, Jelena, Topalov, Drina, Majkić-Singh, Nada, "Association between Val158Met COMT, TNF-alpha-857 C > T, TNFR1 36 A > G, IL-1 alpha 4845 G > T and IL-10-1082 A > G Polymorphisms and Risk of Early-Onset Preeclampsia and Its Complications" in Vojnosanitetski pregled, 74, no. 12 (2017):1155-1161,
https://doi.org/10.2298/VSP160329313K . .

TY  - JOUR
AU  - Jovičić, Snežana
AU  - Majkić-Singh, Nada
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3018
AB  - Medical biochemistry is the usual name for clinical biochemistry or clinical chemistry in Serbia, and medical biochemist is the official name for the clinical chemist (or clinical biochemist). This is the largest sub-discipline of the laboratory medicine in Serbia. It includes all aspects of clinical chemistry, and also laboratory hematology with coagulation, immunology, etc. Medical biochemistry laboratories in Serbia and medical biochemists as a profession are part of Health Care System and their activities are regulated through: the Health Care Law and rules issued by the Chamber of Medical Biochemists of Serbia. The first continuous and organized education for Medical Biochemists (Clinical Chemists) in Serbia dates from 1945, when the Department of Medical Biochemistry was established at the Pharmaceutical Faculty in Belgrade. In 1987 at the same Faculty a five years undergraduate study program was established, educating Medical Biochemists under a special program. Since the academic year 2006/2007 the new five year undergraduate (according to Bologna Declaration) and four-year postgraduate program according to EC4 European Syllabus for Postgraduate Training in Clinical Chemistry and Laboratory Medicine has been established. The Ministry of Education and Ministry of Public Health accredited these programs. There are four requirements for practicing medical biochemistry in the Health Care System: University Diploma of the Faculty of Pharmacy (Study of Medical Biochemistry), successful completion of the professional exam at the Ministry of Health after completion of one additional year of obligatory practical training in the medical biochemistry laboratories, membership in the Serbian Chamber of Medical Biochemists and licence for skilled work issued by the Serbian Chamber of Medical Biochemists. In order to present laboratory medical biochemistry practice in Serbia this paper will be focused on the following: Serbian national legislation, healthcare services organization, sub-disciplines of laboratory medicine and medical biochemistry as the most significant, education in medical biochemistry, conditions for professional practice in medical biochemistry, continuous quality improvement, and accreditation. Serbian healthcare is based on fundamental principles of universal health coverage and solidarity between all citizens.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Medical Biochemistry as Subdiscipline of Laboratory Medicine in Serbia
VL  - 36
IS  - 2
SP  - 177
EP  - 186
DO  - 10.1515/jomb-2017-0010
ER  - 

@article{
author = "Jovičić, Snežana and Majkić-Singh, Nada",
year = "2017",
abstract = "Medical biochemistry is the usual name for clinical biochemistry or clinical chemistry in Serbia, and medical biochemist is the official name for the clinical chemist (or clinical biochemist). This is the largest sub-discipline of the laboratory medicine in Serbia. It includes all aspects of clinical chemistry, and also laboratory hematology with coagulation, immunology, etc. Medical biochemistry laboratories in Serbia and medical biochemists as a profession are part of Health Care System and their activities are regulated through: the Health Care Law and rules issued by the Chamber of Medical Biochemists of Serbia. The first continuous and organized education for Medical Biochemists (Clinical Chemists) in Serbia dates from 1945, when the Department of Medical Biochemistry was established at the Pharmaceutical Faculty in Belgrade. In 1987 at the same Faculty a five years undergraduate study program was established, educating Medical Biochemists under a special program. Since the academic year 2006/2007 the new five year undergraduate (according to Bologna Declaration) and four-year postgraduate program according to EC4 European Syllabus for Postgraduate Training in Clinical Chemistry and Laboratory Medicine has been established. The Ministry of Education and Ministry of Public Health accredited these programs. There are four requirements for practicing medical biochemistry in the Health Care System: University Diploma of the Faculty of Pharmacy (Study of Medical Biochemistry), successful completion of the professional exam at the Ministry of Health after completion of one additional year of obligatory practical training in the medical biochemistry laboratories, membership in the Serbian Chamber of Medical Biochemists and licence for skilled work issued by the Serbian Chamber of Medical Biochemists. In order to present laboratory medical biochemistry practice in Serbia this paper will be focused on the following: Serbian national legislation, healthcare services organization, sub-disciplines of laboratory medicine and medical biochemistry as the most significant, education in medical biochemistry, conditions for professional practice in medical biochemistry, continuous quality improvement, and accreditation. Serbian healthcare is based on fundamental principles of universal health coverage and solidarity between all citizens.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Medical Biochemistry as Subdiscipline of Laboratory Medicine in Serbia",
volume = "36",
number = "2",
pages = "177-186",
doi = "10.1515/jomb-2017-0010"
}

Jovičić, S.,& Majkić-Singh, N.. (2017). Medical Biochemistry as Subdiscipline of Laboratory Medicine in Serbia. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 36(2), 177-186.
https://doi.org/10.1515/jomb-2017-0010

Jovičić S, Majkić-Singh N. Medical Biochemistry as Subdiscipline of Laboratory Medicine in Serbia. in Journal of Medical Biochemistry. 2017;36(2):177-186.
doi:10.1515/jomb-2017-0010 .

Jovičić, Snežana, Majkić-Singh, Nada, "Medical Biochemistry as Subdiscipline of Laboratory Medicine in Serbia" in Journal of Medical Biochemistry, 36, no. 2 (2017):177-186,
https://doi.org/10.1515/jomb-2017-0010 . .

TY  - JOUR
AU  - Milošević-Georgiev, Andrijana
AU  - Krajnović, Dušanka
AU  - Manojlović, Jelena
AU  - Ignjatović, Svetlana
AU  - Majkić-Singh, Nada
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2608
AB  - Introduction: The pharmacists played an important role in the development of biochemistry as applied chemistry in Serbia. What is more, the first seven state chemists in Serbia were pharmacists. State chemists performed the chemical toxicological analysis as well as some medical and biochemical ones. When it comes to the education of medical biochemists as health workers, the period after the beginning of the second half of the twentieth century should be taken into account because that is when the training of pharmaceutical staff of the Faculty of Pharmacy, University of Belgrade, begins on the territory of Serbia. This paper presents the development of medical biochemistry through the development of curriculum, personnel and literature since the foundation of the Faculty of Pharmacy in Serbia until today. Objective: The aim of this paper is to present the historical development of biochemistry at the Faculty of Pharmacy, University of Belgrade, through analysis of three indicators: undergraduate and postgraduate education of medical biochemists, teaching literature and professional associations and trade associations. Method: The method of direct data was applied in this paper. Also, desktop analysis was used for analyzing of secondary data, regulations, curricula, documents and bibliographic material. Desktop research was conducted and based on the following sources: Archives of the University of Belgrade Faculty of Pharmacy, Museum of the History of Pharmacy at the University of Belgrade-Faculty of Pharmacy, the Society of Medical Biochemists of Serbia and the Serbian Chamber of Biochemists. Results and conclusion: The curricula, the Bologna process of improving education, the expansion of the range of subjects, the number of students, professional literature for teaching biochemistry, as well as professional associations and trade associations are presented through the results.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Seventy Years of Biochemical Subjects' Development in Pharmacy Curricula: Experience from Serbia
VL  - 35
IS  - 1
SP  - 69
EP  - 79
DO  - 10.1515/jomb-2015-0018
ER  - 

@article{
author = "Milošević-Georgiev, Andrijana and Krajnović, Dušanka and Manojlović, Jelena and Ignjatović, Svetlana and Majkić-Singh, Nada",
year = "2016",
abstract = "Introduction: The pharmacists played an important role in the development of biochemistry as applied chemistry in Serbia. What is more, the first seven state chemists in Serbia were pharmacists. State chemists performed the chemical toxicological analysis as well as some medical and biochemical ones. When it comes to the education of medical biochemists as health workers, the period after the beginning of the second half of the twentieth century should be taken into account because that is when the training of pharmaceutical staff of the Faculty of Pharmacy, University of Belgrade, begins on the territory of Serbia. This paper presents the development of medical biochemistry through the development of curriculum, personnel and literature since the foundation of the Faculty of Pharmacy in Serbia until today. Objective: The aim of this paper is to present the historical development of biochemistry at the Faculty of Pharmacy, University of Belgrade, through analysis of three indicators: undergraduate and postgraduate education of medical biochemists, teaching literature and professional associations and trade associations. Method: The method of direct data was applied in this paper. Also, desktop analysis was used for analyzing of secondary data, regulations, curricula, documents and bibliographic material. Desktop research was conducted and based on the following sources: Archives of the University of Belgrade Faculty of Pharmacy, Museum of the History of Pharmacy at the University of Belgrade-Faculty of Pharmacy, the Society of Medical Biochemists of Serbia and the Serbian Chamber of Biochemists. Results and conclusion: The curricula, the Bologna process of improving education, the expansion of the range of subjects, the number of students, professional literature for teaching biochemistry, as well as professional associations and trade associations are presented through the results.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Seventy Years of Biochemical Subjects' Development in Pharmacy Curricula: Experience from Serbia",
volume = "35",
number = "1",
pages = "69-79",
doi = "10.1515/jomb-2015-0018"
}

Milošević-Georgiev, A., Krajnović, D., Manojlović, J., Ignjatović, S.,& Majkić-Singh, N.. (2016). Seventy Years of Biochemical Subjects' Development in Pharmacy Curricula: Experience from Serbia. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 35(1), 69-79.
https://doi.org/10.1515/jomb-2015-0018

Milošević-Georgiev A, Krajnović D, Manojlović J, Ignjatović S, Majkić-Singh N. Seventy Years of Biochemical Subjects' Development in Pharmacy Curricula: Experience from Serbia. in Journal of Medical Biochemistry. 2016;35(1):69-79.
doi:10.1515/jomb-2015-0018 .

Milošević-Georgiev, Andrijana, Krajnović, Dušanka, Manojlović, Jelena, Ignjatović, Svetlana, Majkić-Singh, Nada, "Seventy Years of Biochemical Subjects' Development in Pharmacy Curricula: Experience from Serbia" in Journal of Medical Biochemistry, 35, no. 1 (2016):69-79,
https://doi.org/10.1515/jomb-2015-0018 . .

TY  - JOUR
AU  - Krnjeta, Tijana
AU  - Mirković, Ljiljana
AU  - Ignjatović, Svetlana
AU  - Tomasević, Dragana
AU  - Lukić, Jelena
AU  - Topalov, Drina
AU  - Soldatović, Ivan
AU  - Majkić-Singh, Nada
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2638
AB  - Background: Up until now there have been contradictory data about the association between p.Val158Met catechol-O-methyltransferase (COMT) polymorphism and risk of preeclampsia (PE). The goal of this study was to assess the potential correlation between p.Val158Met COMT polymorphism and risk of early-onset PE, risk of a severe form of early-onset PE, as well as risk of small-for-gestationalage (SGA) complicating PE. Methods: The study included 47 early-onset PE patients and 47 control cases. Forty-seven early-onset PE patients were grouped by disease severity (33 patients with a severe form and 14 patients without severe features) and secondly by size for gestational age (12 patients with appropriate-for-gestational-age (AGA) and 35 patients with SGA size). p.Val158Met polymorphism was genotyped by PCR-RFLP analysis. Results: Allele analysis showed significant difference in COMT allele distribution between early-onset PE and control group as well as early-onset PE SGA and controls (p=0.04057 and p=0.0411 respectively). A statistically significant distribution difference between the severe form and form without severe features of early-onset PE patients was not observed (p>0.05). The highest difference ob served was in the allele recessive model where COMT MetMet genotype was associated with decreased risk of early-onset PE (OR=0.281; 95% CI=0.092-0.7836) and PE complications including severe early-onset PE (OR=0.304; 95% CI=0.086-0.944) and SGA early-onset PE (OR=0.284; 95% CI=0.081-0.874). Conclusions: COMT may be used as a candidate gene for early-onset PE and its severe form and SGA complications.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Protective role of maternal P.VAL158MET catechol-o-methyltransferase polymorphism against early-onset preeclampsia and its complications
VL  - 35
IS  - 3
SP  - 312
EP  - 318
DO  - 10.1515/jomb-2016-0013
ER  - 

@article{
author = "Krnjeta, Tijana and Mirković, Ljiljana and Ignjatović, Svetlana and Tomasević, Dragana and Lukić, Jelena and Topalov, Drina and Soldatović, Ivan and Majkić-Singh, Nada",
year = "2016",
abstract = "Background: Up until now there have been contradictory data about the association between p.Val158Met catechol-O-methyltransferase (COMT) polymorphism and risk of preeclampsia (PE). The goal of this study was to assess the potential correlation between p.Val158Met COMT polymorphism and risk of early-onset PE, risk of a severe form of early-onset PE, as well as risk of small-for-gestationalage (SGA) complicating PE. Methods: The study included 47 early-onset PE patients and 47 control cases. Forty-seven early-onset PE patients were grouped by disease severity (33 patients with a severe form and 14 patients without severe features) and secondly by size for gestational age (12 patients with appropriate-for-gestational-age (AGA) and 35 patients with SGA size). p.Val158Met polymorphism was genotyped by PCR-RFLP analysis. Results: Allele analysis showed significant difference in COMT allele distribution between early-onset PE and control group as well as early-onset PE SGA and controls (p=0.04057 and p=0.0411 respectively). A statistically significant distribution difference between the severe form and form without severe features of early-onset PE patients was not observed (p>0.05). The highest difference ob served was in the allele recessive model where COMT MetMet genotype was associated with decreased risk of early-onset PE (OR=0.281; 95% CI=0.092-0.7836) and PE complications including severe early-onset PE (OR=0.304; 95% CI=0.086-0.944) and SGA early-onset PE (OR=0.284; 95% CI=0.081-0.874). Conclusions: COMT may be used as a candidate gene for early-onset PE and its severe form and SGA complications.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Protective role of maternal P.VAL158MET catechol-o-methyltransferase polymorphism against early-onset preeclampsia and its complications",
volume = "35",
number = "3",
pages = "312-318",
doi = "10.1515/jomb-2016-0013"
}

Krnjeta, T., Mirković, L., Ignjatović, S., Tomasević, D., Lukić, J., Topalov, D., Soldatović, I.,& Majkić-Singh, N.. (2016). Protective role of maternal P.VAL158MET catechol-o-methyltransferase polymorphism against early-onset preeclampsia and its complications. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 35(3), 312-318.
https://doi.org/10.1515/jomb-2016-0013

Krnjeta T, Mirković L, Ignjatović S, Tomasević D, Lukić J, Topalov D, Soldatović I, Majkić-Singh N. Protective role of maternal P.VAL158MET catechol-o-methyltransferase polymorphism against early-onset preeclampsia and its complications. in Journal of Medical Biochemistry. 2016;35(3):312-318.
doi:10.1515/jomb-2016-0013 .

Krnjeta, Tijana, Mirković, Ljiljana, Ignjatović, Svetlana, Tomasević, Dragana, Lukić, Jelena, Topalov, Drina, Soldatović, Ivan, Majkić-Singh, Nada, "Protective role of maternal P.VAL158MET catechol-o-methyltransferase polymorphism against early-onset preeclampsia and its complications" in Journal of Medical Biochemistry, 35, no. 3 (2016):312-318,
https://doi.org/10.1515/jomb-2016-0013 . .

TY  - JOUR
AU  - Kangrga, Ranka
AU  - Ignjatović, Svetlana
AU  - Dragasević, Mirjana M.
AU  - Jovičić, Snežana
AU  - Majkić-Singh, Nada
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2546
AB  - Objective: The measurement of pancreatic elastase (PE) in feces is used widely to screen for pancreatic exocrine insufficiency. The aim of our study was to evaluate the relationship of PE with residual beta cell secretion and metabolic control in patients with diabetes mellitus. Method: We determined the presence of PE in specimens via enzyme-linked immunosorbent assay (ELISA), whereas serum fasting glucose, C-peptide, amylase, lipase, triglycerides, total 25(OH)-vitamin D, C-reactive protein (CRP), and hemoglobin A(1c) (HbA(1c)) concentrations were assayed using routine laboratory tests. Results: PE values in 48 patients with diabetes were significantly lower than in 24 healthy volunteers (P = .001). In one-third of participants with diabetes mellitus, PE were less than 200 mu g per g, indicating pancreatic functional insufficiency. Among the patients in the cohort, PE correlated positively with C-peptide levels (P = .04), lipase (P = .009), CRP (P = .04), sex (P = .03), and BMI (P = .02) but not significantly with duration of diabetes (P = .81) or levels of HbA(1c) (P = .87), amylase (P = .06), total 25(OH)-vitamin D (P = .16), or triglycerides (P = .52). Conclusion: Our results demonstrated a strong association of diabetes with low PE levels.
PB  - Oxford Univ Press, Oxford
T2  - Labmedicine
T1  - Pancreatic Elastase Levels in Feces As A Marker of Exocrine Pancreatic Function in Patients With Diabetes Mellitus
VL  - 47
IS  - 2
SP  - 140
EP  - 148
DO  - 10.1093/labmed/lmw015
ER  - 

@article{
author = "Kangrga, Ranka and Ignjatović, Svetlana and Dragasević, Mirjana M. and Jovičić, Snežana and Majkić-Singh, Nada",
year = "2016",
abstract = "Objective: The measurement of pancreatic elastase (PE) in feces is used widely to screen for pancreatic exocrine insufficiency. The aim of our study was to evaluate the relationship of PE with residual beta cell secretion and metabolic control in patients with diabetes mellitus. Method: We determined the presence of PE in specimens via enzyme-linked immunosorbent assay (ELISA), whereas serum fasting glucose, C-peptide, amylase, lipase, triglycerides, total 25(OH)-vitamin D, C-reactive protein (CRP), and hemoglobin A(1c) (HbA(1c)) concentrations were assayed using routine laboratory tests. Results: PE values in 48 patients with diabetes were significantly lower than in 24 healthy volunteers (P = .001). In one-third of participants with diabetes mellitus, PE were less than 200 mu g per g, indicating pancreatic functional insufficiency. Among the patients in the cohort, PE correlated positively with C-peptide levels (P = .04), lipase (P = .009), CRP (P = .04), sex (P = .03), and BMI (P = .02) but not significantly with duration of diabetes (P = .81) or levels of HbA(1c) (P = .87), amylase (P = .06), total 25(OH)-vitamin D (P = .16), or triglycerides (P = .52). Conclusion: Our results demonstrated a strong association of diabetes with low PE levels.",
publisher = "Oxford Univ Press, Oxford",
journal = "Labmedicine",
title = "Pancreatic Elastase Levels in Feces As A Marker of Exocrine Pancreatic Function in Patients With Diabetes Mellitus",
volume = "47",
number = "2",
pages = "140-148",
doi = "10.1093/labmed/lmw015"
}

Kangrga, R., Ignjatović, S., Dragasević, M. M., Jovičić, S.,& Majkić-Singh, N.. (2016). Pancreatic Elastase Levels in Feces As A Marker of Exocrine Pancreatic Function in Patients With Diabetes Mellitus. in Labmedicine
Oxford Univ Press, Oxford., 47(2), 140-148.
https://doi.org/10.1093/labmed/lmw015

Kangrga R, Ignjatović S, Dragasević MM, Jovičić S, Majkić-Singh N. Pancreatic Elastase Levels in Feces As A Marker of Exocrine Pancreatic Function in Patients With Diabetes Mellitus. in Labmedicine. 2016;47(2):140-148.
doi:10.1093/labmed/lmw015 .

Kangrga, Ranka, Ignjatović, Svetlana, Dragasević, Mirjana M., Jovičić, Snežana, Majkić-Singh, Nada, "Pancreatic Elastase Levels in Feces As A Marker of Exocrine Pancreatic Function in Patients With Diabetes Mellitus" in Labmedicine, 47, no. 2 (2016):140-148,
https://doi.org/10.1093/labmed/lmw015 . .

TY  - CONF
AU  - Jovičić, Snežana
AU  - Ignjatović, Svetlana
AU  - Kangrga, Ranka
AU  - Dajak, Marijana
AU  - Majkić-Singh, Nada
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5491
AB  - U kliničkoj praksi koristi se nekoliko skorova za
procenu kratkoročnog (10-godišnjeg) rizika od
pojave različitih oblika kardiovaskularnih bolesti
(KVB) koji se zasnivaju na multivarijabilnim regre-
sionim jednačinama izvedenim iz rezultata praćenja
različitih kohortnih grupa. Međutim, pošto je starost
promenljiva kojoj se dodeljuje najveći broj poena u
modelima 10-godišnjeg rizika, mnoge osobe sa zna
čajnim opterećenjem faktorima rizika imaju kratko-
ročni rizik daleko ispod granice koja uslovljava inten-
zivan tretman, iako njihov dugoročni (30-godišnji)
rizik može biti značajan. Takođe, drugi biomarkeri
mogu da identifikuju osobe sa većim kardiovasku-
larnim rizikom od onog izračunatog primenom skoro-
va kratkoročnog rizika. Cilj rada bio je da se analizira
priroda uticaja ispitivanih biomarkera na kardiovas-
kularni rizik i njihovo grupisanje, kao i povezanost
dobijenih faktora sa kategorizacijom 30-godišnjeg
rizika faktorskom analizom. Pomoću interaktivnog
kalkulatora »30-year risk of cardiovascular disease«
izračunavan je dugoročni 30-godišnji rizik za pojavu
»kompletne« KVB (sve manifestacije KVB) i »teške«
KVB (potencijalno fatalne komplikacije KVB). Analiza
glavnih komponenti je korišćena za ispitivanje
grupisanja markera inflamacije [visoko-osetljivi C-
reaktivni protein (hsCRP), serumski amiloid A (SAA),
fibrinogen, a1-kiseli glikoprotein (A1AGP), haptoglo-
bin, C3 i C4 komponente komplementa], metabo-lizma lipida [non-HDL i LDL holesterol, trigliceridi,
apolipoprotein A-I (apo A-I), apolipoprotein B (apo
B), lipoprotein (a) (Lp(a))], bubrežne [kreatinin,
mokraćna kiselina, cistatin C (Cys-C)] i srčane funk-
cije [N-terminalni pro-natriuretički peptid tip B (NT-
proBNP), visoko-osetljivi srčani troponin T (hs-cTnT)],
dobijenih analizom uzoraka seruma 242 zdrave oso-
be. Faktorskom analizom identifikovano je 5 klastera,
kojima je objašnjeno je 67,4% ukupne varijacije, ras-
poređene na sledeći način 1) 29,7% »sistemska infla-
macija« (hsCRP, fibrinogen, SAA, A1AGP, haptoglo-
bin, C3 i C4 komponenta komplementa); 2) 12,5%
»aterogena dislipidemija« (LDL i non-HDL holesterol,
apo B i trigliceridi); 3) 11,0% »kardiorenalni faktor«
(kreatinin, mokraćna kiselina, Cys-C i hs-cTnT); 4)
7,6% »hemodinamski faktor« (NT-proBNP) i 5) 6,7%
»lipoproteinski faktor« [apo A-I, Lp(a)]. Prediktivne
vrednosti u proceni 30-godišnjeg rizika za »komplet-
nu KVB« i »tešku KVB« su bile značajne za četiri fak-
tora (OR 1,892–5,590; P<0,0001 i OR 2,183–
5,931; P<0,0001, redom), a »hemodinamski fak-
tor« nije imao statistički značajan prediktivni potenci-
jal za vrednosti iznad optimalnih/normalnih za odgo-
varajući pol i starost (P>0,05). Površine ispod ROC
krivih (AUC) modela sa pet faktora u predikciji
povećanog 30-godišnjeg rizika za »kompletnu KVB« i
»tešku KVB« iznosile su 0,881 i 0,888, redom, i nisu
bile statistički značajno različite od multivarijabilnog
logističkog modela od 18 polaznih parametara
(0,892 i 0,901; P>0,05; redom). Sistemska inflama-
cija, aterogena dislipidemija, kardiorenalna funkcija i
lipoproteinski status nezavisno doprinose dugo-
ročnom, 30-godišnjem riziku iznad normalnog/opti-
malnog kako za ozbiljne komplikacije KVB, tako i za
sve vrste kardiovaskularnih komplikacija.
AB  - Several risk score algorithms for short-term (10-
year) cardiovascular risk assessment based on multi-
variable regression equations derived from different
cohorts are being used in clinical practice. However,
since the age is variable with the strongest influence
on short-term risk, many individuals with moderate
increase of other traditional risk factors would have a
10-year risk below cutoff for intensive treatment, but
a significant long-term (30-year) risk. Also, other bio-
markers might identify persons with higher actual
cardiovascular risk compared with calculated using
short-term risk scores. The aim of this study was to
analyze the nature of influence of examined biomark-
ers on cardiovascular risk and their clustering, as well
as relations of identified factors with long-term 30-
year risk categorization, using factor analysis.
Interactive calculator »30-year risk of cardiovascular
disease« was used for long-term 30-year risk calcula-
tion, for both »full CVD« (all manifestations of cardio-
vascular disease) and »hard CVD« (serious manifesta-
tions of CVD). Principal component analysis was used
to investigate clustering of markers of inflammation
[high sensitivity C-reactive protein (hsCRP), serum
amyloid A (SAA), fibrinogen, a1-acid glycoprotein
(A1AGP), haptoglobin, C3 and C4 complement
components], lipid metabolism [non-HDL and LDL
cholesterol, triglycerides, apolipoprotein A-I (apo A-
I), apolipoprotein B (apo B), lipoprotein (a) (Lp(a))], renal [creatinine, uric acid, cystatin C (Cys-C)] and
cardiac function [N-terminal pro-natriuretic peptide
type B (NT-proBNP), high sensitivity cardiac troponin
T (hs-cTnT)], obtained from 242 apparently healthy
individuals. Factor analysis identified five clusters,
which explained 67.4% of the total variance distrib-
uted as follows: 1) 29.7% »systemic inflammation«
(hsCRP, fibrinogen, SAA, A1AGP, haptoglobin, C3,
C4); 2) 12.5% »atherogenic dyslipidemia«, (LDL and
non-HDL cholesterol, apo B, triglycerides); 3) 11.0%
»cardiorenal factor« (creatinine, uric acid, Cys-C, hs-
cTnT); 4) 7.6% »hemodynamic factor« (NT-proBNP);
and 5) 6.7% »lipoprotein factor« [apo A-I, Lp(a)].
When estimating 30-year risk from both »full CVD«
and »hard CVD«, predictive values were significant
for four factors (OR 1.892–5.590, P<0.0001 and
OR 2.183–5.931, P<0.0001, respectively), and
»hemodynamic factor« had no statistical significance
in predicting potential for values above optimal/nor-
mal for corresponding gender and age (P>0.05).
The areas under the receiver operating characteristic
curves (AUCs) of the five factor model in predicting
increased 30-year risk for »full CVD« and »hard CVD«
were 0.881 and 0.888, respectively, which were not
statistically significantly different from AUCs of the
multivariable logistic model of 18 original parameters
(0.892 and 0.901, P>0.05, respectively). Long-
term, 30-year risk above normal/optimal for hard
CVD complications and for all kinds of cardiovascular
complications was independently contributed by sys-
temic inflammation, atherogenic dyslipidemia, car-
diorenal function and lipoprotein status.
PB  - Društvo medicinskih biohemičara Srbije, Beograd
C3  - XIX kongres medicinske i laboratorijske medicine sa me|unarodnim učešćem, 2015, Journal of Medical Chemistry, 34, 1, 2015.
T1  - Faktorska analiza povezanosti inflamatornih, lipidnih, srčanih i bubrežnih biomarkera sa Klasifikacijom dugoročnog 30-godišnjeg kardiovaskularnog rizika
T1  - Factor analysis of association of lipid, inflammatory, cardiac and renal biomarkers with long-term 30-year cardiovascular risk classification
VL  - 34
SP  - 68
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5491
ER  - 

@conference{
author = "Jovičić, Snežana and Ignjatović, Svetlana and Kangrga, Ranka and Dajak, Marijana and Majkić-Singh, Nada",
year = "2015",
abstract = "U kliničkoj praksi koristi se nekoliko skorova za
procenu kratkoročnog (10-godišnjeg) rizika od
pojave različitih oblika kardiovaskularnih bolesti
(KVB) koji se zasnivaju na multivarijabilnim regre-
sionim jednačinama izvedenim iz rezultata praćenja
različitih kohortnih grupa. Međutim, pošto je starost
promenljiva kojoj se dodeljuje najveći broj poena u
modelima 10-godišnjeg rizika, mnoge osobe sa zna
čajnim opterećenjem faktorima rizika imaju kratko-
ročni rizik daleko ispod granice koja uslovljava inten-
zivan tretman, iako njihov dugoročni (30-godišnji)
rizik može biti značajan. Takođe, drugi biomarkeri
mogu da identifikuju osobe sa većim kardiovasku-
larnim rizikom od onog izračunatog primenom skoro-
va kratkoročnog rizika. Cilj rada bio je da se analizira
priroda uticaja ispitivanih biomarkera na kardiovas-
kularni rizik i njihovo grupisanje, kao i povezanost
dobijenih faktora sa kategorizacijom 30-godišnjeg
rizika faktorskom analizom. Pomoću interaktivnog
kalkulatora »30-year risk of cardiovascular disease«
izračunavan je dugoročni 30-godišnji rizik za pojavu
»kompletne« KVB (sve manifestacije KVB) i »teške«
KVB (potencijalno fatalne komplikacije KVB). Analiza
glavnih komponenti je korišćena za ispitivanje
grupisanja markera inflamacije [visoko-osetljivi C-
reaktivni protein (hsCRP), serumski amiloid A (SAA),
fibrinogen, a1-kiseli glikoprotein (A1AGP), haptoglo-
bin, C3 i C4 komponente komplementa], metabo-lizma lipida [non-HDL i LDL holesterol, trigliceridi,
apolipoprotein A-I (apo A-I), apolipoprotein B (apo
B), lipoprotein (a) (Lp(a))], bubrežne [kreatinin,
mokraćna kiselina, cistatin C (Cys-C)] i srčane funk-
cije [N-terminalni pro-natriuretički peptid tip B (NT-
proBNP), visoko-osetljivi srčani troponin T (hs-cTnT)],
dobijenih analizom uzoraka seruma 242 zdrave oso-
be. Faktorskom analizom identifikovano je 5 klastera,
kojima je objašnjeno je 67,4% ukupne varijacije, ras-
poređene na sledeći način 1) 29,7% »sistemska infla-
macija« (hsCRP, fibrinogen, SAA, A1AGP, haptoglo-
bin, C3 i C4 komponenta komplementa); 2) 12,5%
»aterogena dislipidemija« (LDL i non-HDL holesterol,
apo B i trigliceridi); 3) 11,0% »kardiorenalni faktor«
(kreatinin, mokraćna kiselina, Cys-C i hs-cTnT); 4)
7,6% »hemodinamski faktor« (NT-proBNP) i 5) 6,7%
»lipoproteinski faktor« [apo A-I, Lp(a)]. Prediktivne
vrednosti u proceni 30-godišnjeg rizika za »komplet-
nu KVB« i »tešku KVB« su bile značajne za četiri fak-
tora (OR 1,892–5,590; P<0,0001 i OR 2,183–
5,931; P<0,0001, redom), a »hemodinamski fak-
tor« nije imao statistički značajan prediktivni potenci-
jal za vrednosti iznad optimalnih/normalnih za odgo-
varajući pol i starost (P>0,05). Površine ispod ROC
krivih (AUC) modela sa pet faktora u predikciji
povećanog 30-godišnjeg rizika za »kompletnu KVB« i
»tešku KVB« iznosile su 0,881 i 0,888, redom, i nisu
bile statistički značajno različite od multivarijabilnog
logističkog modela od 18 polaznih parametara
(0,892 i 0,901; P>0,05; redom). Sistemska inflama-
cija, aterogena dislipidemija, kardiorenalna funkcija i
lipoproteinski status nezavisno doprinose dugo-
ročnom, 30-godišnjem riziku iznad normalnog/opti-
malnog kako za ozbiljne komplikacije KVB, tako i za
sve vrste kardiovaskularnih komplikacija., Several risk score algorithms for short-term (10-
year) cardiovascular risk assessment based on multi-
variable regression equations derived from different
cohorts are being used in clinical practice. However,
since the age is variable with the strongest influence
on short-term risk, many individuals with moderate
increase of other traditional risk factors would have a
10-year risk below cutoff for intensive treatment, but
a significant long-term (30-year) risk. Also, other bio-
markers might identify persons with higher actual
cardiovascular risk compared with calculated using
short-term risk scores. The aim of this study was to
analyze the nature of influence of examined biomark-
ers on cardiovascular risk and their clustering, as well
as relations of identified factors with long-term 30-
year risk categorization, using factor analysis.
Interactive calculator »30-year risk of cardiovascular
disease« was used for long-term 30-year risk calcula-
tion, for both »full CVD« (all manifestations of cardio-
vascular disease) and »hard CVD« (serious manifesta-
tions of CVD). Principal component analysis was used
to investigate clustering of markers of inflammation
[high sensitivity C-reactive protein (hsCRP), serum
amyloid A (SAA), fibrinogen, a1-acid glycoprotein
(A1AGP), haptoglobin, C3 and C4 complement
components], lipid metabolism [non-HDL and LDL
cholesterol, triglycerides, apolipoprotein A-I (apo A-
I), apolipoprotein B (apo B), lipoprotein (a) (Lp(a))], renal [creatinine, uric acid, cystatin C (Cys-C)] and
cardiac function [N-terminal pro-natriuretic peptide
type B (NT-proBNP), high sensitivity cardiac troponin
T (hs-cTnT)], obtained from 242 apparently healthy
individuals. Factor analysis identified five clusters,
which explained 67.4% of the total variance distrib-
uted as follows: 1) 29.7% »systemic inflammation«
(hsCRP, fibrinogen, SAA, A1AGP, haptoglobin, C3,
C4); 2) 12.5% »atherogenic dyslipidemia«, (LDL and
non-HDL cholesterol, apo B, triglycerides); 3) 11.0%
»cardiorenal factor« (creatinine, uric acid, Cys-C, hs-
cTnT); 4) 7.6% »hemodynamic factor« (NT-proBNP);
and 5) 6.7% »lipoprotein factor« [apo A-I, Lp(a)].
When estimating 30-year risk from both »full CVD«
and »hard CVD«, predictive values were significant
for four factors (OR 1.892–5.590, P<0.0001 and
OR 2.183–5.931, P<0.0001, respectively), and
»hemodynamic factor« had no statistical significance
in predicting potential for values above optimal/nor-
mal for corresponding gender and age (P>0.05).
The areas under the receiver operating characteristic
curves (AUCs) of the five factor model in predicting
increased 30-year risk for »full CVD« and »hard CVD«
were 0.881 and 0.888, respectively, which were not
statistically significantly different from AUCs of the
multivariable logistic model of 18 original parameters
(0.892 and 0.901, P>0.05, respectively). Long-
term, 30-year risk above normal/optimal for hard
CVD complications and for all kinds of cardiovascular
complications was independently contributed by sys-
temic inflammation, atherogenic dyslipidemia, car-
diorenal function and lipoprotein status.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd",
journal = "XIX kongres medicinske i laboratorijske medicine sa me|unarodnim učešćem, 2015, Journal of Medical Chemistry, 34, 1, 2015.",
title = "Faktorska analiza povezanosti inflamatornih, lipidnih, srčanih i bubrežnih biomarkera sa Klasifikacijom dugoročnog 30-godišnjeg kardiovaskularnog rizika, Factor analysis of association of lipid, inflammatory, cardiac and renal biomarkers with long-term 30-year cardiovascular risk classification",
volume = "34",
pages = "68-69",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5491"
}

Jovičić, S., Ignjatović, S., Kangrga, R., Dajak, M.,& Majkić-Singh, N.. (2015). Faktorska analiza povezanosti inflamatornih, lipidnih, srčanih i bubrežnih biomarkera sa Klasifikacijom dugoročnog 30-godišnjeg kardiovaskularnog rizika. in XIX kongres medicinske i laboratorijske medicine sa me|unarodnim učešćem, 2015, Journal of Medical Chemistry, 34, 1, 2015.
Društvo medicinskih biohemičara Srbije, Beograd., 34, 68-69.
https://hdl.handle.net/21.15107/rcub_farfar_5491

Jovičić S, Ignjatović S, Kangrga R, Dajak M, Majkić-Singh N. Faktorska analiza povezanosti inflamatornih, lipidnih, srčanih i bubrežnih biomarkera sa Klasifikacijom dugoročnog 30-godišnjeg kardiovaskularnog rizika. in XIX kongres medicinske i laboratorijske medicine sa me|unarodnim učešćem, 2015, Journal of Medical Chemistry, 34, 1, 2015.. 2015;34:68-69.
https://hdl.handle.net/21.15107/rcub_farfar_5491 .

Jovičić, Snežana, Ignjatović, Svetlana, Kangrga, Ranka, Dajak, Marijana, Majkić-Singh, Nada, "Faktorska analiza povezanosti inflamatornih, lipidnih, srčanih i bubrežnih biomarkera sa Klasifikacijom dugoročnog 30-godišnjeg kardiovaskularnog rizika" in XIX kongres medicinske i laboratorijske medicine sa me|unarodnim učešćem, 2015, Journal of Medical Chemistry, 34, 1, 2015., 34 (2015):68-69,
https://hdl.handle.net/21.15107/rcub_farfar_5491 .

TY  - JOUR
AU  - Beletić, Anđelo
AU  - Mirković, Duško
AU  - Dudvarski-Ilić, Aleksandra
AU  - Milenković, Branislava
AU  - Nagorni-Obradović, Ljudmila
AU  - Đorđević, Valentina
AU  - Ignjatović, Svetlana
AU  - Majkić-Singh, Nada
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2299
AB  - Background: An increased homocysteine (Hcy) concentration may represent a metabolic marker of folate and vitamin B-12 deficiency, both significant public health problems. For different reasons, patients with chronic obstructive pulmonary disease (COPD) are prone to these deficiencies. The study evaluates the reliability of Hcy concentration in predicting folate or vitamin B-12 deficiency in these patients. Methods: A group of 50 COPD patients (28 males/22 females, age ((X) over bar +/- SD=49.0 +/- 14.5) years was enrolled. A chemiluminescent microparticle immunoassay was applied for homocysteine, folate and vitamin B-12 concentration. Kolmogorov-Smirnov, Mann-Whitney U and chi(2) tests, Spearman's correlation and ROC analysis were included in the statistical analysis, with the level of significance set at 0.05. Results: Average (SD) concentrations of folate and vitamin B-12 were 4.13 (2.16) mu g/L and 463.6 (271.0) ng/L, whereas only vitamin B-12 correlated with the Hcy level (P=-0.310 (R=0.029)). Gender related differences were not significant and only a borderline significant correlation between age and folate was confirmed (R=0.279 (P=0.047)). The incidence of folate and vitamin B-12 deficiency differed significantly (P=0.000 and P lt 0.000 for folate and vitamin B12 respectively), depending on the cutoff used for classification (4.4, 6.6 and 8.0 mu g/L folate; 203 and 473 ng/L - vitamin B-12). ROC analyses failed to show any significance of hyperhomocysteinemia as a predictor of folate or vitamin B-12 deficiency. Conclusion: Reliability of the Hcy concentration as a biomarker of folate or vitamin B-12 depletion in COPD patients is not satisfactory, so their deficiency cannot be predicted by the occurrence of HHcy.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Questionable Reliability of Homocysteine As the Metabolic Marker for Folate and Vitamin B12 Deficiency in Patients with Chronic Obstructive Pulmonary Disease
VL  - 34
IS  - 4
SP  - 467
EP  - 472
DO  - 10.2478/jomb-2014-0046
ER  - 

@article{
author = "Beletić, Anđelo and Mirković, Duško and Dudvarski-Ilić, Aleksandra and Milenković, Branislava and Nagorni-Obradović, Ljudmila and Đorđević, Valentina and Ignjatović, Svetlana and Majkić-Singh, Nada",
year = "2015",
abstract = "Background: An increased homocysteine (Hcy) concentration may represent a metabolic marker of folate and vitamin B-12 deficiency, both significant public health problems. For different reasons, patients with chronic obstructive pulmonary disease (COPD) are prone to these deficiencies. The study evaluates the reliability of Hcy concentration in predicting folate or vitamin B-12 deficiency in these patients. Methods: A group of 50 COPD patients (28 males/22 females, age ((X) over bar +/- SD=49.0 +/- 14.5) years was enrolled. A chemiluminescent microparticle immunoassay was applied for homocysteine, folate and vitamin B-12 concentration. Kolmogorov-Smirnov, Mann-Whitney U and chi(2) tests, Spearman's correlation and ROC analysis were included in the statistical analysis, with the level of significance set at 0.05. Results: Average (SD) concentrations of folate and vitamin B-12 were 4.13 (2.16) mu g/L and 463.6 (271.0) ng/L, whereas only vitamin B-12 correlated with the Hcy level (P=-0.310 (R=0.029)). Gender related differences were not significant and only a borderline significant correlation between age and folate was confirmed (R=0.279 (P=0.047)). The incidence of folate and vitamin B-12 deficiency differed significantly (P=0.000 and P lt 0.000 for folate and vitamin B12 respectively), depending on the cutoff used for classification (4.4, 6.6 and 8.0 mu g/L folate; 203 and 473 ng/L - vitamin B-12). ROC analyses failed to show any significance of hyperhomocysteinemia as a predictor of folate or vitamin B-12 deficiency. Conclusion: Reliability of the Hcy concentration as a biomarker of folate or vitamin B-12 depletion in COPD patients is not satisfactory, so their deficiency cannot be predicted by the occurrence of HHcy.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Questionable Reliability of Homocysteine As the Metabolic Marker for Folate and Vitamin B12 Deficiency in Patients with Chronic Obstructive Pulmonary Disease",
volume = "34",
number = "4",
pages = "467-472",
doi = "10.2478/jomb-2014-0046"
}

Beletić, A., Mirković, D., Dudvarski-Ilić, A., Milenković, B., Nagorni-Obradović, L., Đorđević, V., Ignjatović, S.,& Majkić-Singh, N.. (2015). Questionable Reliability of Homocysteine As the Metabolic Marker for Folate and Vitamin B12 Deficiency in Patients with Chronic Obstructive Pulmonary Disease. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 34(4), 467-472.
https://doi.org/10.2478/jomb-2014-0046

Beletić A, Mirković D, Dudvarski-Ilić A, Milenković B, Nagorni-Obradović L, Đorđević V, Ignjatović S, Majkić-Singh N. Questionable Reliability of Homocysteine As the Metabolic Marker for Folate and Vitamin B12 Deficiency in Patients with Chronic Obstructive Pulmonary Disease. in Journal of Medical Biochemistry. 2015;34(4):467-472.
doi:10.2478/jomb-2014-0046 .

Beletić, Anđelo, Mirković, Duško, Dudvarski-Ilić, Aleksandra, Milenković, Branislava, Nagorni-Obradović, Ljudmila, Đorđević, Valentina, Ignjatović, Svetlana, Majkić-Singh, Nada, "Questionable Reliability of Homocysteine As the Metabolic Marker for Folate and Vitamin B12 Deficiency in Patients with Chronic Obstructive Pulmonary Disease" in Journal of Medical Biochemistry, 34, no. 4 (2015):467-472,
https://doi.org/10.2478/jomb-2014-0046 . .

TY  - JOUR
AU  - Beletić, Anđelo
AU  - Dudvarski-Ilić, Aleksandra
AU  - Milenković, Branislava
AU  - Nagorni-Obradović, Ljudmila
AU  - Ljujić, Mila
AU  - Đorđević, Valentina
AU  - Mirković, Duško
AU  - Radojković, Dragica
AU  - Majkić-Singh, Nada
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2204
AB  - Introduction: Alpha-1-antitrypsin deficiency (AATD), genetic risk factor for premature chronic obstructive pulmonary disease (COPD), often remains undetected. The aim of our study was to analyse the effectiveness of an integrative laboratory algorithm for AATD detection in patients diagnosed with COPD by the age of 45 years, in comparison with the screening approach based on AAT concentration measurement alone. Subjects and methods: 50 unrelated patients (28 males / 22 females, age 52 (24-75 years) diagnosed with COPD before the age of 45 years were enrolled. Immunonephelometric assay for alpha-1-antitrypsin (AAT) and PCR-reverse hybridization for Z and S allele were first-line, and isoelectric focusing and DNA sequencing (ABI Prism BigDye) were reflex tests. Results: AATD associated genotypes were detected in 7 patients (5 ZZ, 1 ZM(malton), 1 ZQ0(amersfoort)), 10 were heterozygous carriers (8 MZ and 2 MS genotypes) and 33 were without AATD (MM genotype). Carriers and patients without AATD had comparable AAT concentrations (P = 0.125). In majority of participants (48) first line tests were sufficient to analyze AATD presence. In two remaining cases reflex tests identified rare alleles, M-malton and Q0(amersfoort), the later one being reported for the first time in Serbian population. Detection rate did not differ between algorithm and screening both for AATD (P = 0.500) and carriers (P = 0.063). Conclusion: There is a high prevalence of AATD affected subjects and carriers in a group of patients with premature COPD. The use of integrative laboratory algorithm does not improve the effectiveness of AATD detection in comparison with the screening based on AAT concentration alone.
PB  - Croatian Soc Medical Biochemists, Zagreb
T2  - Biochemia Medica
T1  - Is an integrative laboratory algorithm more effective in detecting alpha-1-antitrypsin deficiency in patients with premature chronic obstructive pulmonary disease than AAT concentration based screening approach?
VL  - 24
IS  - 2
SP  - 293
EP  - 298
DO  - 10.11613/BM.2014.032
ER  - 

@article{
author = "Beletić, Anđelo and Dudvarski-Ilić, Aleksandra and Milenković, Branislava and Nagorni-Obradović, Ljudmila and Ljujić, Mila and Đorđević, Valentina and Mirković, Duško and Radojković, Dragica and Majkić-Singh, Nada",
year = "2014",
abstract = "Introduction: Alpha-1-antitrypsin deficiency (AATD), genetic risk factor for premature chronic obstructive pulmonary disease (COPD), often remains undetected. The aim of our study was to analyse the effectiveness of an integrative laboratory algorithm for AATD detection in patients diagnosed with COPD by the age of 45 years, in comparison with the screening approach based on AAT concentration measurement alone. Subjects and methods: 50 unrelated patients (28 males / 22 females, age 52 (24-75 years) diagnosed with COPD before the age of 45 years were enrolled. Immunonephelometric assay for alpha-1-antitrypsin (AAT) and PCR-reverse hybridization for Z and S allele were first-line, and isoelectric focusing and DNA sequencing (ABI Prism BigDye) were reflex tests. Results: AATD associated genotypes were detected in 7 patients (5 ZZ, 1 ZM(malton), 1 ZQ0(amersfoort)), 10 were heterozygous carriers (8 MZ and 2 MS genotypes) and 33 were without AATD (MM genotype). Carriers and patients without AATD had comparable AAT concentrations (P = 0.125). In majority of participants (48) first line tests were sufficient to analyze AATD presence. In two remaining cases reflex tests identified rare alleles, M-malton and Q0(amersfoort), the later one being reported for the first time in Serbian population. Detection rate did not differ between algorithm and screening both for AATD (P = 0.500) and carriers (P = 0.063). Conclusion: There is a high prevalence of AATD affected subjects and carriers in a group of patients with premature COPD. The use of integrative laboratory algorithm does not improve the effectiveness of AATD detection in comparison with the screening based on AAT concentration alone.",
publisher = "Croatian Soc Medical Biochemists, Zagreb",
journal = "Biochemia Medica",
title = "Is an integrative laboratory algorithm more effective in detecting alpha-1-antitrypsin deficiency in patients with premature chronic obstructive pulmonary disease than AAT concentration based screening approach?",
volume = "24",
number = "2",
pages = "293-298",
doi = "10.11613/BM.2014.032"
}

Beletić, A., Dudvarski-Ilić, A., Milenković, B., Nagorni-Obradović, L., Ljujić, M., Đorđević, V., Mirković, D., Radojković, D.,& Majkić-Singh, N.. (2014). Is an integrative laboratory algorithm more effective in detecting alpha-1-antitrypsin deficiency in patients with premature chronic obstructive pulmonary disease than AAT concentration based screening approach?. in Biochemia Medica
Croatian Soc Medical Biochemists, Zagreb., 24(2), 293-298.
https://doi.org/10.11613/BM.2014.032

Beletić A, Dudvarski-Ilić A, Milenković B, Nagorni-Obradović L, Ljujić M, Đorđević V, Mirković D, Radojković D, Majkić-Singh N. Is an integrative laboratory algorithm more effective in detecting alpha-1-antitrypsin deficiency in patients with premature chronic obstructive pulmonary disease than AAT concentration based screening approach?. in Biochemia Medica. 2014;24(2):293-298.
doi:10.11613/BM.2014.032 .

Beletić, Anđelo, Dudvarski-Ilić, Aleksandra, Milenković, Branislava, Nagorni-Obradović, Ljudmila, Ljujić, Mila, Đorđević, Valentina, Mirković, Duško, Radojković, Dragica, Majkić-Singh, Nada, "Is an integrative laboratory algorithm more effective in detecting alpha-1-antitrypsin deficiency in patients with premature chronic obstructive pulmonary disease than AAT concentration based screening approach?" in Biochemia Medica, 24, no. 2 (2014):293-298,
https://doi.org/10.11613/BM.2014.032 . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Ignjatović, Svetlana
AU  - Žarković, Miloš
AU  - Jovičić, Snežana
AU  - Radosavljević, Branimir
AU  - Singh, Sandra
AU  - Majkić-Singh, Nada
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2099
AB  - Objectives. Defining adequate reference limits (RLs) for thyroid hormones is an important task for support monitoring and the treatment of subclinical thyroid disease. We determined whether there are age-related RLs for thyroid parameters in male and female outpatients free of overt thyroid disease. Design. We analyzed 22,860 results (11,440 male and 11,420 female outpatients above the age of 18) for thyrotropin (TSH), free thyroxine (fT4) and total triiodothyronine (T3) that were stored in our laboratory information system between 2008 and 2011. We calculated the 2.5th and 97.5th centiles for the analyzed thyroid parameters. Results. Our results indicate higher TSH levels with ageing, with a significant difference (p  lt  0.05) between the 97.5th centiles for males and females older than 70 (5.07 mIU/L and 4.10 mIU/L), but also a significant difference between male and female fT4 from 31 to 40 and from 41 to 50 years old (18.4 vs 14.9 pmol/L and 19.0 vs 15.9 pmol/L, p  lt  0.05), respectively. Overall indirect estimates of the 97.5th centiles for TSH for males and females were not significantly different and were below the generally recommended upper limit (4.01 mIU/L and 4.20 mIU/L, respectively). In addition, we found no statistically signifi cant change in mean T3 values in the analyzed population. Conclusions. This cross-sectional study indicates change in TSH and fT4 levels with ageing and gender-related upper limits. This suggests that by using indirect estimation a laboratory could provide clinicians with more accurate gender- and age-specific RLs for thyroid parameters.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Scandinavian Journal of Clinical and Laboratory Investigation
T1  - Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results
VL  - 74
IS  - 5
SP  - 378
EP  - 384
DO  - 10.3109/00365513.2014.898324
ER  - 

@article{
author = "Milinković, Neda and Ignjatović, Svetlana and Žarković, Miloš and Jovičić, Snežana and Radosavljević, Branimir and Singh, Sandra and Majkić-Singh, Nada",
year = "2014",
abstract = "Objectives. Defining adequate reference limits (RLs) for thyroid hormones is an important task for support monitoring and the treatment of subclinical thyroid disease. We determined whether there are age-related RLs for thyroid parameters in male and female outpatients free of overt thyroid disease. Design. We analyzed 22,860 results (11,440 male and 11,420 female outpatients above the age of 18) for thyrotropin (TSH), free thyroxine (fT4) and total triiodothyronine (T3) that were stored in our laboratory information system between 2008 and 2011. We calculated the 2.5th and 97.5th centiles for the analyzed thyroid parameters. Results. Our results indicate higher TSH levels with ageing, with a significant difference (p  lt  0.05) between the 97.5th centiles for males and females older than 70 (5.07 mIU/L and 4.10 mIU/L), but also a significant difference between male and female fT4 from 31 to 40 and from 41 to 50 years old (18.4 vs 14.9 pmol/L and 19.0 vs 15.9 pmol/L, p  lt  0.05), respectively. Overall indirect estimates of the 97.5th centiles for TSH for males and females were not significantly different and were below the generally recommended upper limit (4.01 mIU/L and 4.20 mIU/L, respectively). In addition, we found no statistically signifi cant change in mean T3 values in the analyzed population. Conclusions. This cross-sectional study indicates change in TSH and fT4 levels with ageing and gender-related upper limits. This suggests that by using indirect estimation a laboratory could provide clinicians with more accurate gender- and age-specific RLs for thyroid parameters.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Scandinavian Journal of Clinical and Laboratory Investigation",
title = "Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results",
volume = "74",
number = "5",
pages = "378-384",
doi = "10.3109/00365513.2014.898324"
}

Milinković, N., Ignjatović, S., Žarković, M., Jovičić, S., Radosavljević, B., Singh, S.,& Majkić-Singh, N.. (2014). Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results. in Scandinavian Journal of Clinical and Laboratory Investigation
Taylor & Francis Ltd, Abingdon., 74(5), 378-384.
https://doi.org/10.3109/00365513.2014.898324

Milinković N, Ignjatović S, Žarković M, Jovičić S, Radosavljević B, Singh S, Majkić-Singh N. Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results. in Scandinavian Journal of Clinical and Laboratory Investigation. 2014;74(5):378-384.
doi:10.3109/00365513.2014.898324 .

Milinković, Neda, Ignjatović, Svetlana, Žarković, Miloš, Jovičić, Snežana, Radosavljević, Branimir, Singh, Sandra, Majkić-Singh, Nada, "Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results" in Scandinavian Journal of Clinical and Laboratory Investigation, 74, no. 5 (2014):378-384,
https://doi.org/10.3109/00365513.2014.898324 . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Ignjatović, Svetlana
AU  - Žarković, Miloš
AU  - Radosavljević, Branimir
AU  - Majkić-Singh, Nada
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2081
AB  - Background: The aim of this work was to determine indirect reference intervals from patients' results obtained during routine laboratory work. This could be an accurate alternative to the laborious and expensive job of producing reference intervals for populations according to international recommendations. Methods: All the results for thyrotropin (TSH), total and free thyroxine, and triiodothyronine (T4, fT4, T3, and fT3) stored in our laboratory information system between 2008 and 2011 were included in this study. We used logarithmic transformation of the raw data to exclude outliers. After visual observation of the data distribution, we estimated non-parametric reference intervals. A standard normal deviation test was performed to test the significance of differences between subgroups. Results: There was no significant difference in the serum levels of the analyzed thyroid parameters, so we calculated combined reference values. However, we found a significant difference in TSH values between ambulatory and hospitalized patients, but only in 2011. Indirect reference values for TSH, T4, fT4, T3 and fT3 were 0.42 - 3.67 mIU/L, 66.0 - 136.10 nmol/L, 10.20 - 18.40 pmol/L, 1.10 - 2.39 nmol/L, and 3.17 - 5.59 pmol/L, respectively. Conclusions: The indirect determination of laboratory-specific reference intervals using patients' laboratory data is a relatively easy and inexpensive method. Also, indirect reference limits will be more precise and true if skewness and kurtosis of the distribution are not too large.
PB  - Clin Lab Publ, Heidelberg
T2  - Clinical Laboratory
T1  - Indirect Estimation of Reference Intervals for Thyroid Parameters
VL  - 60
IS  - 7
SP  - 1083
EP  - 1089
DO  - 10.7754/Clin.Lab.2013.130733
ER  - 

@article{
author = "Milinković, Neda and Ignjatović, Svetlana and Žarković, Miloš and Radosavljević, Branimir and Majkić-Singh, Nada",
year = "2014",
abstract = "Background: The aim of this work was to determine indirect reference intervals from patients' results obtained during routine laboratory work. This could be an accurate alternative to the laborious and expensive job of producing reference intervals for populations according to international recommendations. Methods: All the results for thyrotropin (TSH), total and free thyroxine, and triiodothyronine (T4, fT4, T3, and fT3) stored in our laboratory information system between 2008 and 2011 were included in this study. We used logarithmic transformation of the raw data to exclude outliers. After visual observation of the data distribution, we estimated non-parametric reference intervals. A standard normal deviation test was performed to test the significance of differences between subgroups. Results: There was no significant difference in the serum levels of the analyzed thyroid parameters, so we calculated combined reference values. However, we found a significant difference in TSH values between ambulatory and hospitalized patients, but only in 2011. Indirect reference values for TSH, T4, fT4, T3 and fT3 were 0.42 - 3.67 mIU/L, 66.0 - 136.10 nmol/L, 10.20 - 18.40 pmol/L, 1.10 - 2.39 nmol/L, and 3.17 - 5.59 pmol/L, respectively. Conclusions: The indirect determination of laboratory-specific reference intervals using patients' laboratory data is a relatively easy and inexpensive method. Also, indirect reference limits will be more precise and true if skewness and kurtosis of the distribution are not too large.",
publisher = "Clin Lab Publ, Heidelberg",
journal = "Clinical Laboratory",
title = "Indirect Estimation of Reference Intervals for Thyroid Parameters",
volume = "60",
number = "7",
pages = "1083-1089",
doi = "10.7754/Clin.Lab.2013.130733"
}

Milinković, N., Ignjatović, S., Žarković, M., Radosavljević, B.,& Majkić-Singh, N.. (2014). Indirect Estimation of Reference Intervals for Thyroid Parameters. in Clinical Laboratory
Clin Lab Publ, Heidelberg., 60(7), 1083-1089.
https://doi.org/10.7754/Clin.Lab.2013.130733

Milinković N, Ignjatović S, Žarković M, Radosavljević B, Majkić-Singh N. Indirect Estimation of Reference Intervals for Thyroid Parameters. in Clinical Laboratory. 2014;60(7):1083-1089.
doi:10.7754/Clin.Lab.2013.130733 .

Milinković, Neda, Ignjatović, Svetlana, Žarković, Miloš, Radosavljević, Branimir, Majkić-Singh, Nada, "Indirect Estimation of Reference Intervals for Thyroid Parameters" in Clinical Laboratory, 60, no. 7 (2014):1083-1089,
https://doi.org/10.7754/Clin.Lab.2013.130733 . .

TY  - JOUR
AU  - Pejović, Janko
AU  - Ignjatović, Svetlana
AU  - Dajak, Marijana
AU  - Majkić-Singh, Nada
AU  - Vučinić, Žarko
AU  - Pavlović, Miroslav
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1854
AB  - Background/Aim. Identification of patients with arterial hypertension and a possible onset of heart failure by determining the concentration of N-terminal pro-B-type natriuretic peptide (NT-proBNP) enables timely intensification of treatment and allows clinicians to prescribe and implement optimal and appropriate care. The aim of this study was to evaluate NT-proBNP in patients with longstanding hypertension and in patients with signs of hypertensive cardiomyopathy. Methods. The study involved 3 groups, with 50 subjects each: "healthy" persons (control group), patients with hypertension and normal left ventricular systolic function (group 1) and patients with longstanding hypertension and signs of hypertensive cardiomyopathy with impaired left ventricular systolic function (group 2). We measured levels of NT-proBNP, C-reactive protein and creatinine according to the manufacturer's instructions. All the patients were clinically examined including physical examination of the heart with blood pressure, pulse rate, electrocardiogram (ECG) and echocardiogram. Results. Our results showed that the determined parameters generally differed significantly (Student's t-test) among the groups. The mean (+/- SD) values of NT-proBNP in the control group, group 1 and group 2 were: 2.794 (+/- 1.515) pmol/L, 9.575 (+/- 5.449) pmol/L and 204.60 (84,93) pmol/L, respectively. NT-proBNP correlated significantly with the determined parameters both in the group 1 and the group 2. In the group 1, the highest correlation was obtained with C-reactive protein (r = 0.8424). In the group 2, the highest correlation was obtained with ejection fraction (r = -0.9111). NT-proBNP showed progressive increase in proportion to the New York Heart Association (NYHA) classification. The patients in the- group 2 who belonged to the II and III NYHA class had significantly higher levels of NT-proBNP than those in the NYHA class I (ANOVA test,p = 0.001). Conclusion. The obtained results suggest that NT-proBNP is a useful biomarker in the treatment of patients with longstanding hypertension who are at risk for heart failure.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Correlation of N-terminal pro-B-type natriuretic peptide with clinical parameters in patients with hypertension
VL  - 70
IS  - 8
SP  - 728
EP  - 734
DO  - 10.2298/VSP110322048P
ER  - 

@article{
author = "Pejović, Janko and Ignjatović, Svetlana and Dajak, Marijana and Majkić-Singh, Nada and Vučinić, Žarko and Pavlović, Miroslav",
year = "2013",
abstract = "Background/Aim. Identification of patients with arterial hypertension and a possible onset of heart failure by determining the concentration of N-terminal pro-B-type natriuretic peptide (NT-proBNP) enables timely intensification of treatment and allows clinicians to prescribe and implement optimal and appropriate care. The aim of this study was to evaluate NT-proBNP in patients with longstanding hypertension and in patients with signs of hypertensive cardiomyopathy. Methods. The study involved 3 groups, with 50 subjects each: "healthy" persons (control group), patients with hypertension and normal left ventricular systolic function (group 1) and patients with longstanding hypertension and signs of hypertensive cardiomyopathy with impaired left ventricular systolic function (group 2). We measured levels of NT-proBNP, C-reactive protein and creatinine according to the manufacturer's instructions. All the patients were clinically examined including physical examination of the heart with blood pressure, pulse rate, electrocardiogram (ECG) and echocardiogram. Results. Our results showed that the determined parameters generally differed significantly (Student's t-test) among the groups. The mean (+/- SD) values of NT-proBNP in the control group, group 1 and group 2 were: 2.794 (+/- 1.515) pmol/L, 9.575 (+/- 5.449) pmol/L and 204.60 (84,93) pmol/L, respectively. NT-proBNP correlated significantly with the determined parameters both in the group 1 and the group 2. In the group 1, the highest correlation was obtained with C-reactive protein (r = 0.8424). In the group 2, the highest correlation was obtained with ejection fraction (r = -0.9111). NT-proBNP showed progressive increase in proportion to the New York Heart Association (NYHA) classification. The patients in the- group 2 who belonged to the II and III NYHA class had significantly higher levels of NT-proBNP than those in the NYHA class I (ANOVA test,p = 0.001). Conclusion. The obtained results suggest that NT-proBNP is a useful biomarker in the treatment of patients with longstanding hypertension who are at risk for heart failure.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Correlation of N-terminal pro-B-type natriuretic peptide with clinical parameters in patients with hypertension",
volume = "70",
number = "8",
pages = "728-734",
doi = "10.2298/VSP110322048P"
}

Pejović, J., Ignjatović, S., Dajak, M., Majkić-Singh, N., Vučinić, Ž.,& Pavlović, M.. (2013). Correlation of N-terminal pro-B-type natriuretic peptide with clinical parameters in patients with hypertension. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 70(8), 728-734.
https://doi.org/10.2298/VSP110322048P

Pejović J, Ignjatović S, Dajak M, Majkić-Singh N, Vučinić Ž, Pavlović M. Correlation of N-terminal pro-B-type natriuretic peptide with clinical parameters in patients with hypertension. in Vojnosanitetski pregled. 2013;70(8):728-734.
doi:10.2298/VSP110322048P .

Pejović, Janko, Ignjatović, Svetlana, Dajak, Marijana, Majkić-Singh, Nada, Vučinić, Žarko, Pavlović, Miroslav, "Correlation of N-terminal pro-B-type natriuretic peptide with clinical parameters in patients with hypertension" in Vojnosanitetski pregled, 70, no. 8 (2013):728-734,
https://doi.org/10.2298/VSP110322048P . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Ignjatović, Svetlana
AU  - Majkić-Singh, Nada
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1934
AB  - It has been proposed that apoptosis is one of the mechanisms involved in the generation of antiphospholipid antibodies. The presence of antiphospholipid antibodies is the main laboratory criterion for a definite diagnosis of the antiphospholipid syndrome. Annexinopathies are disorders characterized by deregulation of annexins expression levels and function. Annexin A5 has been used as an agent for molecular imaging techniques (visualization of phosphatidylserine-expressing apoptotic cells) in vitro and in vivo in animal models and in patients (injection of human recombinant anxA5 into the patient's circulation). Although the determination of titers of anti-annexin A5 antibodies is not mandatory for the diagnosis of the antiphospholipid syndrome, it was reported that patients with primary antiphospholipid syndrome with a history of recurrent abortions had elevated titers of anti-annexin A5 antibodies, while the presence of thromboses was not associated with elevated levels of these antibodies.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Apoptosis, annexin a5 and anti-Annexin a5 antibodies in the antiphospholipid syndrome
VL  - 32
IS  - 2
SP  - 89
EP  - 95
DO  - 10.2478/jomb-2013-0014
ER  - 

@article{
author = "Becarević, Mirjana and Ignjatović, Svetlana and Majkić-Singh, Nada",
year = "2013",
abstract = "It has been proposed that apoptosis is one of the mechanisms involved in the generation of antiphospholipid antibodies. The presence of antiphospholipid antibodies is the main laboratory criterion for a definite diagnosis of the antiphospholipid syndrome. Annexinopathies are disorders characterized by deregulation of annexins expression levels and function. Annexin A5 has been used as an agent for molecular imaging techniques (visualization of phosphatidylserine-expressing apoptotic cells) in vitro and in vivo in animal models and in patients (injection of human recombinant anxA5 into the patient's circulation). Although the determination of titers of anti-annexin A5 antibodies is not mandatory for the diagnosis of the antiphospholipid syndrome, it was reported that patients with primary antiphospholipid syndrome with a history of recurrent abortions had elevated titers of anti-annexin A5 antibodies, while the presence of thromboses was not associated with elevated levels of these antibodies.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Apoptosis, annexin a5 and anti-Annexin a5 antibodies in the antiphospholipid syndrome",
volume = "32",
number = "2",
pages = "89-95",
doi = "10.2478/jomb-2013-0014"
}

Becarević, M., Ignjatović, S.,& Majkić-Singh, N.. (2013). Apoptosis, annexin a5 and anti-Annexin a5 antibodies in the antiphospholipid syndrome. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 32(2), 89-95.
https://doi.org/10.2478/jomb-2013-0014

Becarević M, Ignjatović S, Majkić-Singh N. Apoptosis, annexin a5 and anti-Annexin a5 antibodies in the antiphospholipid syndrome. in Journal of Medical Biochemistry. 2013;32(2):89-95.
doi:10.2478/jomb-2013-0014 .

Becarević, Mirjana, Ignjatović, Svetlana, Majkić-Singh, Nada, "Apoptosis, annexin a5 and anti-Annexin a5 antibodies in the antiphospholipid syndrome" in Journal of Medical Biochemistry, 32, no. 2 (2013):89-95,
https://doi.org/10.2478/jomb-2013-0014 . .

TY  - JOUR
AU  - Stanković, Sanja
AU  - Ašanin, Milika
AU  - Trifunović, Danijela
AU  - Majkić-Singh, Nada
AU  - Ignjatović, Svetlana
AU  - Mrdović, Igor
AU  - Matić, Dragan
AU  - Savić, Lidija
AU  - Marinković, Jelena
AU  - Ostojić, Miodrag
AU  - Vasiljević, Zorana
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1752
AB  - Objectives: To analyze the prognostic value of myeloperoxidase (MPO) in relation to in-hospital mortality and to identify the optimum time point for sampling in patients with the first anterior ST-segment elevation myocardial infarction (STEMI) treated by primary percutaneous coronary intervention (pPCI). Design and methods: A total of 100 consecutive patients with the first anterior STEMI undergoing pPCI were included. Blood samples were collected at baseline, 4, 8, 12, 18, 24,48 and 168 hours (h) after pPCI. Results: MPO concentrations have showed a biphasic pattern over time; the highest MPO levels were at 4 h and 2411 after pPCI. In-hospital mortality was 6%. MPO at 24 h significantly correlated with troponin I as well as heart failure. After multivariate adjustment, MPO at 24 h was an independent predictor of the in-hospital mortality (OR 3.34, 95% CI 1.13-9.86, P = 0.029). Conclusions: In patients with the first anterior STEMI treated by pPCI. MPO at 24 h after procedure was an independent predictor of the in-hospital mortality.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Clinical Biochemistry
T1  - Time-dependent changes of myeloperoxidase in relation to in-hospital mortality in patients with the first anterior ST-segment elevation myocardial infarction treated by primary percutaneous coronary intervention
VL  - 45
IS  - 7-8
SP  - 547
EP  - 551
DO  - 10.1016/j.clinbiochem.2012.02.015
ER  - 

@article{
author = "Stanković, Sanja and Ašanin, Milika and Trifunović, Danijela and Majkić-Singh, Nada and Ignjatović, Svetlana and Mrdović, Igor and Matić, Dragan and Savić, Lidija and Marinković, Jelena and Ostojić, Miodrag and Vasiljević, Zorana",
year = "2012",
abstract = "Objectives: To analyze the prognostic value of myeloperoxidase (MPO) in relation to in-hospital mortality and to identify the optimum time point for sampling in patients with the first anterior ST-segment elevation myocardial infarction (STEMI) treated by primary percutaneous coronary intervention (pPCI). Design and methods: A total of 100 consecutive patients with the first anterior STEMI undergoing pPCI were included. Blood samples were collected at baseline, 4, 8, 12, 18, 24,48 and 168 hours (h) after pPCI. Results: MPO concentrations have showed a biphasic pattern over time; the highest MPO levels were at 4 h and 2411 after pPCI. In-hospital mortality was 6%. MPO at 24 h significantly correlated with troponin I as well as heart failure. After multivariate adjustment, MPO at 24 h was an independent predictor of the in-hospital mortality (OR 3.34, 95% CI 1.13-9.86, P = 0.029). Conclusions: In patients with the first anterior STEMI treated by pPCI. MPO at 24 h after procedure was an independent predictor of the in-hospital mortality.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Clinical Biochemistry",
title = "Time-dependent changes of myeloperoxidase in relation to in-hospital mortality in patients with the first anterior ST-segment elevation myocardial infarction treated by primary percutaneous coronary intervention",
volume = "45",
number = "7-8",
pages = "547-551",
doi = "10.1016/j.clinbiochem.2012.02.015"
}

Stanković, S., Ašanin, M., Trifunović, D., Majkić-Singh, N., Ignjatović, S., Mrdović, I., Matić, D., Savić, L., Marinković, J., Ostojić, M.,& Vasiljević, Z.. (2012). Time-dependent changes of myeloperoxidase in relation to in-hospital mortality in patients with the first anterior ST-segment elevation myocardial infarction treated by primary percutaneous coronary intervention. in Clinical Biochemistry
Pergamon-Elsevier Science Ltd, Oxford., 45(7-8), 547-551.
https://doi.org/10.1016/j.clinbiochem.2012.02.015

Stanković S, Ašanin M, Trifunović D, Majkić-Singh N, Ignjatović S, Mrdović I, Matić D, Savić L, Marinković J, Ostojić M, Vasiljević Z. Time-dependent changes of myeloperoxidase in relation to in-hospital mortality in patients with the first anterior ST-segment elevation myocardial infarction treated by primary percutaneous coronary intervention. in Clinical Biochemistry. 2012;45(7-8):547-551.
doi:10.1016/j.clinbiochem.2012.02.015 .

Stanković, Sanja, Ašanin, Milika, Trifunović, Danijela, Majkić-Singh, Nada, Ignjatović, Svetlana, Mrdović, Igor, Matić, Dragan, Savić, Lidija, Marinković, Jelena, Ostojić, Miodrag, Vasiljević, Zorana, "Time-dependent changes of myeloperoxidase in relation to in-hospital mortality in patients with the first anterior ST-segment elevation myocardial infarction treated by primary percutaneous coronary intervention" in Clinical Biochemistry, 45, no. 7-8 (2012):547-551,
https://doi.org/10.1016/j.clinbiochem.2012.02.015 . .

TY  - JOUR
AU  - Stanković, Sanja
AU  - Ašanin, Milika
AU  - Majkić-Singh, Nada
AU  - Ignjatović, Svetlana
AU  - Mihailović, Mirjana
AU  - Nikolajević, Ivica
AU  - Mrdović, Igor
AU  - Matić, Dragan
AU  - Savić, Lidija
AU  - Marinković, Jelena
AU  - Ostojić, Miodrag
AU  - Vasiljević, Zorana
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1701
AB  - Background: The predictive value of myeloperoxidase (MPO) in ST-segment elevation myocardial infarction (STEM I) treated by primary percutaneous coronary intervention (PCI) has not been established. The aim of the present study was to investigate MPO as a predictor of in-hospital mortality in STEMI patients treated by primary PCI. Methods: Study population consisted of 189 STEMI patients having undergone primary PCI. Plasma MPO level was measured 24 hours after symptom onset using chemiluminescent microparticle immunoassay (Abbott Diagnostics, Germany). The Receiver Operating Characteristic analysis was performed to identify the most useful MPO cut-off level for the prediction of in-hospital mortality. The patients were divided into two groups according to the cut-off MPO level: high MPO group (>= 840 pmol/L, n = 65) and low M PO group ( lt 840 pmol/L, n = 124). Results: The high M PO group had significantly more frequent anterior wall infarctions (p lt 0.001) and Killip class >1 on admission (p=0.013) as well as lower left ventricular ejection fraction (LVEF) (p=0.011) and higher B-type natriuretic peptide (BNP) (p=0.029) than the low MPO group. The incidence of in-hospital mortality was 5.8% and was significantly higher in the high M PO group (13.8%) than in the low MPO group (1.6%) (p=0.001). Multiple logistic regression analysis identified the plasma MPO level as an independent predictor of in-hospital mortality (OR 3.88, 95%CI 1.13 - 13.34, p=0.031). Conclusions: Plasma M PO level independently predicts in-hospital mortality in STEMI patients treated by primary PCI. (Clin. Lab. 2012;58:125-131)
PB  - Clin Lab Publ, Heidelberg
T2  - Clinical Laboratory
T1  - The Usefulness of Myeloperoxidase in Prediction of In-Hospital Mortality in Patients with ST-Segment Elevation Myocardial Infarction Treated by Primary Percutaneous Coronary Intervention
VL  - 58
IS  - 1-2
SP  - 125
EP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1701
ER  - 

@article{
author = "Stanković, Sanja and Ašanin, Milika and Majkić-Singh, Nada and Ignjatović, Svetlana and Mihailović, Mirjana and Nikolajević, Ivica and Mrdović, Igor and Matić, Dragan and Savić, Lidija and Marinković, Jelena and Ostojić, Miodrag and Vasiljević, Zorana",
year = "2012",
abstract = "Background: The predictive value of myeloperoxidase (MPO) in ST-segment elevation myocardial infarction (STEM I) treated by primary percutaneous coronary intervention (PCI) has not been established. The aim of the present study was to investigate MPO as a predictor of in-hospital mortality in STEMI patients treated by primary PCI. Methods: Study population consisted of 189 STEMI patients having undergone primary PCI. Plasma MPO level was measured 24 hours after symptom onset using chemiluminescent microparticle immunoassay (Abbott Diagnostics, Germany). The Receiver Operating Characteristic analysis was performed to identify the most useful MPO cut-off level for the prediction of in-hospital mortality. The patients were divided into two groups according to the cut-off MPO level: high MPO group (>= 840 pmol/L, n = 65) and low M PO group ( lt 840 pmol/L, n = 124). Results: The high M PO group had significantly more frequent anterior wall infarctions (p lt 0.001) and Killip class >1 on admission (p=0.013) as well as lower left ventricular ejection fraction (LVEF) (p=0.011) and higher B-type natriuretic peptide (BNP) (p=0.029) than the low MPO group. The incidence of in-hospital mortality was 5.8% and was significantly higher in the high M PO group (13.8%) than in the low MPO group (1.6%) (p=0.001). Multiple logistic regression analysis identified the plasma MPO level as an independent predictor of in-hospital mortality (OR 3.88, 95%CI 1.13 - 13.34, p=0.031). Conclusions: Plasma M PO level independently predicts in-hospital mortality in STEMI patients treated by primary PCI. (Clin. Lab. 2012;58:125-131)",
publisher = "Clin Lab Publ, Heidelberg",
journal = "Clinical Laboratory",
title = "The Usefulness of Myeloperoxidase in Prediction of In-Hospital Mortality in Patients with ST-Segment Elevation Myocardial Infarction Treated by Primary Percutaneous Coronary Intervention",
volume = "58",
number = "1-2",
pages = "125-131",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1701"
}

Stanković, S., Ašanin, M., Majkić-Singh, N., Ignjatović, S., Mihailović, M., Nikolajević, I., Mrdović, I., Matić, D., Savić, L., Marinković, J., Ostojić, M.,& Vasiljević, Z.. (2012). The Usefulness of Myeloperoxidase in Prediction of In-Hospital Mortality in Patients with ST-Segment Elevation Myocardial Infarction Treated by Primary Percutaneous Coronary Intervention. in Clinical Laboratory
Clin Lab Publ, Heidelberg., 58(1-2), 125-131.
https://hdl.handle.net/21.15107/rcub_farfar_1701

Stanković S, Ašanin M, Majkić-Singh N, Ignjatović S, Mihailović M, Nikolajević I, Mrdović I, Matić D, Savić L, Marinković J, Ostojić M, Vasiljević Z. The Usefulness of Myeloperoxidase in Prediction of In-Hospital Mortality in Patients with ST-Segment Elevation Myocardial Infarction Treated by Primary Percutaneous Coronary Intervention. in Clinical Laboratory. 2012;58(1-2):125-131.
https://hdl.handle.net/21.15107/rcub_farfar_1701 .

Stanković, Sanja, Ašanin, Milika, Majkić-Singh, Nada, Ignjatović, Svetlana, Mihailović, Mirjana, Nikolajević, Ivica, Mrdović, Igor, Matić, Dragan, Savić, Lidija, Marinković, Jelena, Ostojić, Miodrag, Vasiljević, Zorana, "The Usefulness of Myeloperoxidase in Prediction of In-Hospital Mortality in Patients with ST-Segment Elevation Myocardial Infarction Treated by Primary Percutaneous Coronary Intervention" in Clinical Laboratory, 58, no. 1-2 (2012):125-131,
https://hdl.handle.net/21.15107/rcub_farfar_1701 .

TY  - JOUR
AU  - Stanković, Sanja
AU  - Ašanin, Milika
AU  - Trifunović, Danijela
AU  - Majkić-Singh, Nada
AU  - Miljković, Aleksandar
AU  - Ignjatović, Svetlana
AU  - Mrdović, Igor
AU  - Matić, Dragan
AU  - Savić, Lidija
AU  - Ostojić, Miodrag
AU  - Vasiljević, Zorana
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1687
AB  - Background: Lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) has been suggested as an inflammatory marker of cardiovascular risk. The predictive value of Lp-PLA(2) in ST-segment elevation myocardial infarction (STEMI) treated by primary percutaneous coronary intervention (PCI) has not been established. The aim of this study was to determine whether plasma Lp-PLA(2) is a predictor of a major adverse cardiac event (MACE) in patients with the first anterior STEMI treated by primary PCI. Methods: This study consisted of 100 consecutive patients with first anterior STEMI who underwent primary PCI within 6 hours of the symptom onset. Plasma Lp-PLA(2) level was measured on admission using a turbidimetric immunoassay (diaDexus, Inc., USA). The Receiver Operating Characteristic analysis was performed to identify the most useful Lp-PLA(2) cut-off level for the prediction of MACE. The patients were divided into two groups according to the cut-off Lp-PLA(2) level: high Lp-PLA(2) group (>= 463 ng/mL, n = 33) and low Lp-PLA(2) group ( lt  463 ng/mL, n = 67). MACE was defined as cardiac death, non-fatal reinfarction, and target vessel revascularization. Results: Patients in the high Lp-PLA(2) group had significantly higher total-, LDL-cholesterol, apolipoprotein B levels, and significantly lower estimated glomerular filtration rates compared with the low Lp-PLA(2) group. The incidence of 30-day mortality was 18.2% (6/33) in high Lp-PLA(2) group, while in the low Lp-PLA(2) group no patient died (p  lt  0.001). The 30-day MACE occurred in 24.2% of the high Lp-PLA(2) group and 3% of the low Lp-PLA(2) group (p = 0.001). Multiple logistic regression analysis identified the plasma Lp-PLA(2) level as an independent predictor of MACE (OR 1.011, 95%CI 1.001 - 1.013, p = 0.037). Conclusions: In patients with first anterior STEMI treated by primary PCI, the plasma Lp-PLA(2) level is an independent predictor of 30-day MACE. (Clin. Lab. 2012;58:1135-1144. DOI: 10.7754/Clin.Lab.2012.111102)
PB  - Clin Lab Publ, Heidelberg
T2  - Clinical Laboratory
T1  - Utility of Lipoprotein-Associated Phospholipase A(2) for Prediction of 30-day Major Adverse Coronary Event in Patients with the First Anterior ST-Segment Elevation Myocardial Infarction Treated by Primary Percutaneous Coronary Intervention
VL  - 58
IS  - 11-12
SP  - 1135
EP  - 1144
DO  - 10.7754/Clin.Lab.2012.111102
ER  - 

@article{
author = "Stanković, Sanja and Ašanin, Milika and Trifunović, Danijela and Majkić-Singh, Nada and Miljković, Aleksandar and Ignjatović, Svetlana and Mrdović, Igor and Matić, Dragan and Savić, Lidija and Ostojić, Miodrag and Vasiljević, Zorana",
year = "2012",
abstract = "Background: Lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) has been suggested as an inflammatory marker of cardiovascular risk. The predictive value of Lp-PLA(2) in ST-segment elevation myocardial infarction (STEMI) treated by primary percutaneous coronary intervention (PCI) has not been established. The aim of this study was to determine whether plasma Lp-PLA(2) is a predictor of a major adverse cardiac event (MACE) in patients with the first anterior STEMI treated by primary PCI. Methods: This study consisted of 100 consecutive patients with first anterior STEMI who underwent primary PCI within 6 hours of the symptom onset. Plasma Lp-PLA(2) level was measured on admission using a turbidimetric immunoassay (diaDexus, Inc., USA). The Receiver Operating Characteristic analysis was performed to identify the most useful Lp-PLA(2) cut-off level for the prediction of MACE. The patients were divided into two groups according to the cut-off Lp-PLA(2) level: high Lp-PLA(2) group (>= 463 ng/mL, n = 33) and low Lp-PLA(2) group ( lt  463 ng/mL, n = 67). MACE was defined as cardiac death, non-fatal reinfarction, and target vessel revascularization. Results: Patients in the high Lp-PLA(2) group had significantly higher total-, LDL-cholesterol, apolipoprotein B levels, and significantly lower estimated glomerular filtration rates compared with the low Lp-PLA(2) group. The incidence of 30-day mortality was 18.2% (6/33) in high Lp-PLA(2) group, while in the low Lp-PLA(2) group no patient died (p  lt  0.001). The 30-day MACE occurred in 24.2% of the high Lp-PLA(2) group and 3% of the low Lp-PLA(2) group (p = 0.001). Multiple logistic regression analysis identified the plasma Lp-PLA(2) level as an independent predictor of MACE (OR 1.011, 95%CI 1.001 - 1.013, p = 0.037). Conclusions: In patients with first anterior STEMI treated by primary PCI, the plasma Lp-PLA(2) level is an independent predictor of 30-day MACE. (Clin. Lab. 2012;58:1135-1144. DOI: 10.7754/Clin.Lab.2012.111102)",
publisher = "Clin Lab Publ, Heidelberg",
journal = "Clinical Laboratory",
title = "Utility of Lipoprotein-Associated Phospholipase A(2) for Prediction of 30-day Major Adverse Coronary Event in Patients with the First Anterior ST-Segment Elevation Myocardial Infarction Treated by Primary Percutaneous Coronary Intervention",
volume = "58",
number = "11-12",
pages = "1135-1144",
doi = "10.7754/Clin.Lab.2012.111102"
}

Stanković, S., Ašanin, M., Trifunović, D., Majkić-Singh, N., Miljković, A., Ignjatović, S., Mrdović, I., Matić, D., Savić, L., Ostojić, M.,& Vasiljević, Z.. (2012). Utility of Lipoprotein-Associated Phospholipase A(2) for Prediction of 30-day Major Adverse Coronary Event in Patients with the First Anterior ST-Segment Elevation Myocardial Infarction Treated by Primary Percutaneous Coronary Intervention. in Clinical Laboratory
Clin Lab Publ, Heidelberg., 58(11-12), 1135-1144.
https://doi.org/10.7754/Clin.Lab.2012.111102

Stanković S, Ašanin M, Trifunović D, Majkić-Singh N, Miljković A, Ignjatović S, Mrdović I, Matić D, Savić L, Ostojić M, Vasiljević Z. Utility of Lipoprotein-Associated Phospholipase A(2) for Prediction of 30-day Major Adverse Coronary Event in Patients with the First Anterior ST-Segment Elevation Myocardial Infarction Treated by Primary Percutaneous Coronary Intervention. in Clinical Laboratory. 2012;58(11-12):1135-1144.
doi:10.7754/Clin.Lab.2012.111102 .

Stanković, Sanja, Ašanin, Milika, Trifunović, Danijela, Majkić-Singh, Nada, Miljković, Aleksandar, Ignjatović, Svetlana, Mrdović, Igor, Matić, Dragan, Savić, Lidija, Ostojić, Miodrag, Vasiljević, Zorana, "Utility of Lipoprotein-Associated Phospholipase A(2) for Prediction of 30-day Major Adverse Coronary Event in Patients with the First Anterior ST-Segment Elevation Myocardial Infarction Treated by Primary Percutaneous Coronary Intervention" in Clinical Laboratory, 58, no. 11-12 (2012):1135-1144,
https://doi.org/10.7754/Clin.Lab.2012.111102 . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Majkić-Singh, Nada
AU  - Ignjatović, Svetlana
AU  - Lezaić, Višnja D.
AU  - Pejanović, Svetlana D.
AU  - Jovanović, Dijana B.
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1638
AB  - Background: Bone alkaline phosphatase (BALP) is a direct and independent indicator of impaired bone turnover. We intended to find out whether there are any significant changes in BALP and iPTH levels, in comparison to total Ca, total Mg, inorganic P, total alkaline phosphatase (ALP), and tartrate resistant acid phosphatase (TRAP) in predialysis and dialysis patients. Methods: Out of 266 patients investigated, 114 were on continuous ambulatory peritoneal dialysis, 112 were on maintenance haemodialysis, while 40 predialysis patients had end stage renal disease. The parameters were analysed according to the manufacturers' instructions. Results: Correlations were established for the bone marker concentrations analysed among the studied groups. The largest ranges were determined for BALP and iPTH. Predialysis and dialysis patients showed very low levels of BALP. Dialysis patients had lower levels of iPTH (p lt 0.001), while in predialysis patients the levels were significantly higher (p lt 0.05) than recommended for low bone turnover, according to K/DOQI. Conclusions: The observations made in this study identify BALP as a good indicator of decreased bone turnover in predialysis and dialysis patients. However, in order to reveal a difference between bone activity and the level of parathyroid activity and its effect on bone turnover, it is always necessary to observe both BALP and iPTH levels. (Clin. Lab. 2012;58:747-753. DOI: 10.7754/Clin.Lab.2011.110816)
PB  - Clin Lab Publ, Heidelberg
T2  - Clinical Laboratory
T1  - Correlation of Bone Alkaline Phosphatase and iPTH with Some Basic Biochemical Markers in Predialysis and Dialysis Patients
VL  - 58
IS  - 7-8
SP  - 747
EP  - 753
DO  - 10.7754/Clin.Lab.2011.110816
ER  - 

@article{
author = "Milinković, Neda and Majkić-Singh, Nada and Ignjatović, Svetlana and Lezaić, Višnja D. and Pejanović, Svetlana D. and Jovanović, Dijana B.",
year = "2012",
abstract = "Background: Bone alkaline phosphatase (BALP) is a direct and independent indicator of impaired bone turnover. We intended to find out whether there are any significant changes in BALP and iPTH levels, in comparison to total Ca, total Mg, inorganic P, total alkaline phosphatase (ALP), and tartrate resistant acid phosphatase (TRAP) in predialysis and dialysis patients. Methods: Out of 266 patients investigated, 114 were on continuous ambulatory peritoneal dialysis, 112 were on maintenance haemodialysis, while 40 predialysis patients had end stage renal disease. The parameters were analysed according to the manufacturers' instructions. Results: Correlations were established for the bone marker concentrations analysed among the studied groups. The largest ranges were determined for BALP and iPTH. Predialysis and dialysis patients showed very low levels of BALP. Dialysis patients had lower levels of iPTH (p lt 0.001), while in predialysis patients the levels were significantly higher (p lt 0.05) than recommended for low bone turnover, according to K/DOQI. Conclusions: The observations made in this study identify BALP as a good indicator of decreased bone turnover in predialysis and dialysis patients. However, in order to reveal a difference between bone activity and the level of parathyroid activity and its effect on bone turnover, it is always necessary to observe both BALP and iPTH levels. (Clin. Lab. 2012;58:747-753. DOI: 10.7754/Clin.Lab.2011.110816)",
publisher = "Clin Lab Publ, Heidelberg",
journal = "Clinical Laboratory",
title = "Correlation of Bone Alkaline Phosphatase and iPTH with Some Basic Biochemical Markers in Predialysis and Dialysis Patients",
volume = "58",
number = "7-8",
pages = "747-753",
doi = "10.7754/Clin.Lab.2011.110816"
}

Milinković, N., Majkić-Singh, N., Ignjatović, S., Lezaić, V. D., Pejanović, S. D.,& Jovanović, D. B.. (2012). Correlation of Bone Alkaline Phosphatase and iPTH with Some Basic Biochemical Markers in Predialysis and Dialysis Patients. in Clinical Laboratory
Clin Lab Publ, Heidelberg., 58(7-8), 747-753.
https://doi.org/10.7754/Clin.Lab.2011.110816

Milinković N, Majkić-Singh N, Ignjatović S, Lezaić VD, Pejanović SD, Jovanović DB. Correlation of Bone Alkaline Phosphatase and iPTH with Some Basic Biochemical Markers in Predialysis and Dialysis Patients. in Clinical Laboratory. 2012;58(7-8):747-753.
doi:10.7754/Clin.Lab.2011.110816 .

Milinković, Neda, Majkić-Singh, Nada, Ignjatović, Svetlana, Lezaić, Višnja D., Pejanović, Svetlana D., Jovanović, Dijana B., "Correlation of Bone Alkaline Phosphatase and iPTH with Some Basic Biochemical Markers in Predialysis and Dialysis Patients" in Clinical Laboratory, 58, no. 7-8 (2012):747-753,
https://doi.org/10.7754/Clin.Lab.2011.110816 . .

TY  - JOUR
AU  - Jovičić, Snežana
AU  - Ignjatović, Svetlana
AU  - Kangrga, Ranka
AU  - Beletić, Anđelo
AU  - Mirković, Duško
AU  - Majkić-Singh, Nada
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1656
AB  - Determination of 25-hydroxyvitamin D [25(OH)D] represents a unique challenge, considering its lipophilic nature. Considering the widespread prevalence of vitamin D deficiency, which leads to increasing number of requests for 25(OH)D determination, immunoassay measurements adjusted to automated analyzers are being developed. Because of the variability among assays, it is often difficult to monitor vitamin D status and supplementation. The aim of this study was to compare the results of two immunoassays with high performance liquid chromatography with ultraviolet detection (HPLC-UV). Also, the aim was to estimate vitamin D status, since up to date the prevalence of vitamin D deficiency in Serbia was not examined. We have evaluated analytical characteristics of two automated immunoassays for 25(OH)D determination, from Roche (Cobas® e601) and Abbott (Architect). For comparison studies we used HPLC analysis of 25-(OH)-Vitamin D3/D2 from Chromsystems (Waters isocratic system). In order to estimate vitamin D status in general population, we have searched the database of the laboratory information system and analyzed the data from 533 patients whose 25(OH)D was determined together with intact parathyroid hormone (iPTH). For imprecision assessment, four serum pools were prepared with following 25(OH)D concentrations: 35 nmol/L, ?50 nmol/L, ?75 nmol/L and ?125 nmol/L. Obtai ned CVs for Roche method were 1.5-2.8% for within-run and 4.0-6.7% for between-run imprecision. For Abbott method, CVs were 0.7-4.4% for withinrun and 3.8-7.2% for between-run imprecision. Inaccuracy was analyzed with commercial control sera. Obtained deviations from target value were 2.1% for Roche assay and 1.3-1.5% for Abbott method, and were not statistically significant (P>0.05). Comparison of Roche and HPLC-UV methods using Passing-Bablok regression analysis gave the following equation for the regression line y=0.937x+9.518 (r=0.739; n=97) and the regression line equation from the comparison of Abbott and HPLC-UV methods was y=0.745x+10.343 (r=0.793; n=97). Mean difference and SD for Bland-Altman plot were -4.5 nmol/L and 21.75 nmo/L, respectively for Roche method and 6.4 nmol/L and 18.8 nmol/L, respectively for Abbott. Statistical analysis (Chi-square test) of frequency distribution among different vitamin D status categories ( lt 25 nmol/L severe deficiency, 25-50 nmol/L deficiency, 50-75 nmol/L insufficiency and >75 nmol/L sufficiency) showed that the frequency distribution obtained with Abbott method was significantly different from the distribution of the HPLC results, in contrast to Roche results frequency distribution which did not differ significantly. Also, statistical analysis of the agreement between the three methods for each vitamin D status category showed that results of both Roche and Abbott methods were significantly higher than HPLC in the two deficiency categories (P=0.005 for Roche, P=0.0407 for Abbott), and in the sufficiency category Abbott method significantly underestimated concentration of 25(OH)D compared to HPLC results (P lt 0.0001). Median population values of 25(OH)D and iPTH were 41.8 nmol/L and 76.6 ng/L, respectively. ANOVA analyses showed significant (P lt 0.05) decrease in iPTH and Ca2+ concentrations across the 25(OH)D concentration categories. Stepwise multiple linear regression analysis indicated independent correlation of iPTH with 25(OH)D concentration (b=-0.290, P=0.0008). Also, one-way ANOVA with Student-Newman-Keuls test demonstrated that 25(OH)D concentrations measured in summer and autumn were significantly (P lt 0.001) higher compared to those determined in winter and spring. Despite acceptable imprecision and inaccuracy of both examined methods, results obtained with them did not correlate well with HPLC-UV (r lt 0.9), which was used as a reference. However, methods showed satisfactory ability to classify patients into vitamin D status categories, which is important for diagnosis of vitamin D deficiency and therapy follow-up. About two thirds (68.5%) of the examined population had vitamin D deficiency (25(OH)D lt 50 nmol/L) and only 8% had sufficient 25(OH)D concentration (>75 nmol/L).
AB  - Određivanje 25-hidroksivitamina D [25(OH)D] predstavlja jedinstven izazov, s obzirom da je visoko lipofilno jedinjenje. Visoka prevalencija deficijencije vitamina D uzrok je povećanja broja zahteva za određivanjem 25(OH)D, zbog čega se razvijaju imunohemijske metode prilagođene automatizovanim sistemima. Često je teško pratiti status vitamina D i suplementaciju zbog varijabilnosti između testova. Cilj ove studije bio je da se uporede rezultati dve imunohemijske metode sa tečnom hromatografijom visoke efikasnosti sa detekcijom u ultraljubičastom delu spektra (HPLC-UV). Takođe, cilj je bio i procena statusa vitamina D, pošto do sada nije ispitivana prevalencija deficijencije vitamina D u Srbiji. Ispitivane su karakteristike dve imunohemijske metode za određivanje 25(OH)D, proizvođača Roche (analizator Cobas® e601) i Abbott (na analizatoru Architect). Metode su poređene sa rezultatima HPLC analize korišćenjem 25-(OH)-Vitamin D3/D2 reagenasa firme Chromsystems (Waters izokratski sistem). Da bi se procenio status vitamina D u opštoj populaciji, pretražena je baza podataka laboratorijskog informacionog sistema i analizirani su rezultati 533 pacijenata kojima je određen 25(OH)D zajedno sa intaktnim paratiroidnim hormonom (iPTH). Pripremljena su četiri serumska pool-a sa koncentracijama 25(OH)D ? 35 nmol/L, ?50 nmol/L, ?75 nmol/L i ?125 nmol/L za procenu nepreciznosti imunohemijskih određivanja. Dobijeni koeficijenti varijacije za Roche metodu su se kretali u opsegu 1,5-2,8% u seriji i 4,0-6,7% između serija. Za Abbott metodu su koficijenti varijacije iznosili 0,7-4,4% u seriji i 3,8-7,2% između serija. Netačnost je ispitivana pomoću komercijalnih kontrolnih uzoraka. Dobijena odstupanja od deklarisane vrednosti su iznosila 2,1% za Roche i 1,3-1,5% za Abbott, i nisu bila statistički značajna (P>0,05). Poređenjem Roche i HPLC-UV metoda pomoću Passing-Bablok regresione analize dobijena je sledeća regresiona jednačina y=0,937x+9,518 (r=0,739; n=97), dok regresiona jednačina dobijena poređenjem Abbott i HPLC-UV metoda glasi y=0,745x+10,343 (r=0,793; n=97). Srednja vrednost razlika na Bland-Altman dijagramu razlika i standardna devijacija su iznosile -4,5 nmol/L i 21,75 nmo/L, redom, za Roche metodu i 6,4 nmol/L i 18,8 nmol/L, re dom, za Abbott metodu. Statistička analiza (Chi-kvadrat test) distribucije frekvencija među različitim kategorijama statusa vitamina D ( lt 25 nmol/L teška deficijencija, 25-50 nmol/L deficijencija, 50-75 nmol/L insuficijencija i >75 nmol/L preporučena koncentracija) je pokazala da je distribucija frekvencija dobijena Abbott metodom značajno različita od distribucije HPLC rezultata, za razliku od ras po dele frekvencija dobijene Roche metodom koja se nije značajno razlikovala. Takođe, statistička analiza slaganja između ispitivane tri metode u svakoj od kategorija statusa vitamina D je pokazala da su rezultati i Roche i Abbott metoda značajno veći od HPLC-UV u kategorijama deficijencije vitamina D (P=0,005 za Roche; P=0,0407 za Abbott), i u kategoriji sa preporučenom koncentracijom vitamina D Abbott metoda je značajno potcenjivala koncentraciju 25(OH)D u poređenju sa HPLC rezultatima (P lt 0,0001). Medijana za 25(OH)D u ispitivanoj populaciji bila je 41,8 nmol/L, i 76,6 za iPTH. ANOVA analiza je pokazala značajan pad (P lt 0,05) koncentracija iPTH i jonizovanog kalcijuma između kategorija koncentracija 25(OH)D. Multiplomlinearnom regresionom analizom utvrđena je ne zavisna korelacija između koncentracija iPTH i 25(OH)D (b =-0,290; P=0,0008). Takođe, ANOVA za jedan kriterijum klasifikacije sa Student-Newman-Keuls testom je pokazala da su koncentracije 25(OH)D određene u leto i jesen značajno više (P lt 0,001) u poređenju sa onima određenim u zimu ili proleće. Uprkos prihvatljivoj nepreciznosti i netačnosti obe ispitivane imunohemijske metode, dobijeni rezultati nisu u zadovoljavajućoj korelaciji sa HPLC-UV metodom (r lt 0,9), koja je korišćena kao referentna u ovom slučaju. Uprkos ovoj činjenici, metode su pokazale zadovoljavajuću sposobnost klasifikacije pacijenata u kategorije statusa vitamina D, što je važno za dijagnozu deficijencije vitamina D i praćenje terapije. Oko dve trećine (68,5%) ispitivane populacije je imalo deficijenciju vitamina D (25(OH)D lt 50 nmol/L) i samo 8% je imalo preporučenu koncentraciju 25(OH)D (>75 nmol/L).
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience
T1  - Poređenje tri različite metode za određivanje 25(OH)-vitamina D i statusa vitamina D u opštoj populaciji - srpsko iskustvo
VL  - 31
IS  - 4
SP  - 347
EP  - 357
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1656
ER  - 

@article{
author = "Jovičić, Snežana and Ignjatović, Svetlana and Kangrga, Ranka and Beletić, Anđelo and Mirković, Duško and Majkić-Singh, Nada",
year = "2012",
abstract = "Determination of 25-hydroxyvitamin D [25(OH)D] represents a unique challenge, considering its lipophilic nature. Considering the widespread prevalence of vitamin D deficiency, which leads to increasing number of requests for 25(OH)D determination, immunoassay measurements adjusted to automated analyzers are being developed. Because of the variability among assays, it is often difficult to monitor vitamin D status and supplementation. The aim of this study was to compare the results of two immunoassays with high performance liquid chromatography with ultraviolet detection (HPLC-UV). Also, the aim was to estimate vitamin D status, since up to date the prevalence of vitamin D deficiency in Serbia was not examined. We have evaluated analytical characteristics of two automated immunoassays for 25(OH)D determination, from Roche (Cobas® e601) and Abbott (Architect). For comparison studies we used HPLC analysis of 25-(OH)-Vitamin D3/D2 from Chromsystems (Waters isocratic system). In order to estimate vitamin D status in general population, we have searched the database of the laboratory information system and analyzed the data from 533 patients whose 25(OH)D was determined together with intact parathyroid hormone (iPTH). For imprecision assessment, four serum pools were prepared with following 25(OH)D concentrations: 35 nmol/L, ?50 nmol/L, ?75 nmol/L and ?125 nmol/L. Obtai ned CVs for Roche method were 1.5-2.8% for within-run and 4.0-6.7% for between-run imprecision. For Abbott method, CVs were 0.7-4.4% for withinrun and 3.8-7.2% for between-run imprecision. Inaccuracy was analyzed with commercial control sera. Obtained deviations from target value were 2.1% for Roche assay and 1.3-1.5% for Abbott method, and were not statistically significant (P>0.05). Comparison of Roche and HPLC-UV methods using Passing-Bablok regression analysis gave the following equation for the regression line y=0.937x+9.518 (r=0.739; n=97) and the regression line equation from the comparison of Abbott and HPLC-UV methods was y=0.745x+10.343 (r=0.793; n=97). Mean difference and SD for Bland-Altman plot were -4.5 nmol/L and 21.75 nmo/L, respectively for Roche method and 6.4 nmol/L and 18.8 nmol/L, respectively for Abbott. Statistical analysis (Chi-square test) of frequency distribution among different vitamin D status categories ( lt 25 nmol/L severe deficiency, 25-50 nmol/L deficiency, 50-75 nmol/L insufficiency and >75 nmol/L sufficiency) showed that the frequency distribution obtained with Abbott method was significantly different from the distribution of the HPLC results, in contrast to Roche results frequency distribution which did not differ significantly. Also, statistical analysis of the agreement between the three methods for each vitamin D status category showed that results of both Roche and Abbott methods were significantly higher than HPLC in the two deficiency categories (P=0.005 for Roche, P=0.0407 for Abbott), and in the sufficiency category Abbott method significantly underestimated concentration of 25(OH)D compared to HPLC results (P lt 0.0001). Median population values of 25(OH)D and iPTH were 41.8 nmol/L and 76.6 ng/L, respectively. ANOVA analyses showed significant (P lt 0.05) decrease in iPTH and Ca2+ concentrations across the 25(OH)D concentration categories. Stepwise multiple linear regression analysis indicated independent correlation of iPTH with 25(OH)D concentration (b=-0.290, P=0.0008). Also, one-way ANOVA with Student-Newman-Keuls test demonstrated that 25(OH)D concentrations measured in summer and autumn were significantly (P lt 0.001) higher compared to those determined in winter and spring. Despite acceptable imprecision and inaccuracy of both examined methods, results obtained with them did not correlate well with HPLC-UV (r lt 0.9), which was used as a reference. However, methods showed satisfactory ability to classify patients into vitamin D status categories, which is important for diagnosis of vitamin D deficiency and therapy follow-up. About two thirds (68.5%) of the examined population had vitamin D deficiency (25(OH)D lt 50 nmol/L) and only 8% had sufficient 25(OH)D concentration (>75 nmol/L)., Određivanje 25-hidroksivitamina D [25(OH)D] predstavlja jedinstven izazov, s obzirom da je visoko lipofilno jedinjenje. Visoka prevalencija deficijencije vitamina D uzrok je povećanja broja zahteva za određivanjem 25(OH)D, zbog čega se razvijaju imunohemijske metode prilagođene automatizovanim sistemima. Često je teško pratiti status vitamina D i suplementaciju zbog varijabilnosti između testova. Cilj ove studije bio je da se uporede rezultati dve imunohemijske metode sa tečnom hromatografijom visoke efikasnosti sa detekcijom u ultraljubičastom delu spektra (HPLC-UV). Takođe, cilj je bio i procena statusa vitamina D, pošto do sada nije ispitivana prevalencija deficijencije vitamina D u Srbiji. Ispitivane su karakteristike dve imunohemijske metode za određivanje 25(OH)D, proizvođača Roche (analizator Cobas® e601) i Abbott (na analizatoru Architect). Metode su poređene sa rezultatima HPLC analize korišćenjem 25-(OH)-Vitamin D3/D2 reagenasa firme Chromsystems (Waters izokratski sistem). Da bi se procenio status vitamina D u opštoj populaciji, pretražena je baza podataka laboratorijskog informacionog sistema i analizirani su rezultati 533 pacijenata kojima je određen 25(OH)D zajedno sa intaktnim paratiroidnim hormonom (iPTH). Pripremljena su četiri serumska pool-a sa koncentracijama 25(OH)D ? 35 nmol/L, ?50 nmol/L, ?75 nmol/L i ?125 nmol/L za procenu nepreciznosti imunohemijskih određivanja. Dobijeni koeficijenti varijacije za Roche metodu su se kretali u opsegu 1,5-2,8% u seriji i 4,0-6,7% između serija. Za Abbott metodu su koficijenti varijacije iznosili 0,7-4,4% u seriji i 3,8-7,2% između serija. Netačnost je ispitivana pomoću komercijalnih kontrolnih uzoraka. Dobijena odstupanja od deklarisane vrednosti su iznosila 2,1% za Roche i 1,3-1,5% za Abbott, i nisu bila statistički značajna (P>0,05). Poređenjem Roche i HPLC-UV metoda pomoću Passing-Bablok regresione analize dobijena je sledeća regresiona jednačina y=0,937x+9,518 (r=0,739; n=97), dok regresiona jednačina dobijena poređenjem Abbott i HPLC-UV metoda glasi y=0,745x+10,343 (r=0,793; n=97). Srednja vrednost razlika na Bland-Altman dijagramu razlika i standardna devijacija su iznosile -4,5 nmol/L i 21,75 nmo/L, redom, za Roche metodu i 6,4 nmol/L i 18,8 nmol/L, re dom, za Abbott metodu. Statistička analiza (Chi-kvadrat test) distribucije frekvencija među različitim kategorijama statusa vitamina D ( lt 25 nmol/L teška deficijencija, 25-50 nmol/L deficijencija, 50-75 nmol/L insuficijencija i >75 nmol/L preporučena koncentracija) je pokazala da je distribucija frekvencija dobijena Abbott metodom značajno različita od distribucije HPLC rezultata, za razliku od ras po dele frekvencija dobijene Roche metodom koja se nije značajno razlikovala. Takođe, statistička analiza slaganja između ispitivane tri metode u svakoj od kategorija statusa vitamina D je pokazala da su rezultati i Roche i Abbott metoda značajno veći od HPLC-UV u kategorijama deficijencije vitamina D (P=0,005 za Roche; P=0,0407 za Abbott), i u kategoriji sa preporučenom koncentracijom vitamina D Abbott metoda je značajno potcenjivala koncentraciju 25(OH)D u poređenju sa HPLC rezultatima (P lt 0,0001). Medijana za 25(OH)D u ispitivanoj populaciji bila je 41,8 nmol/L, i 76,6 za iPTH. ANOVA analiza je pokazala značajan pad (P lt 0,05) koncentracija iPTH i jonizovanog kalcijuma između kategorija koncentracija 25(OH)D. Multiplomlinearnom regresionom analizom utvrđena je ne zavisna korelacija između koncentracija iPTH i 25(OH)D (b =-0,290; P=0,0008). Takođe, ANOVA za jedan kriterijum klasifikacije sa Student-Newman-Keuls testom je pokazala da su koncentracije 25(OH)D određene u leto i jesen značajno više (P lt 0,001) u poređenju sa onima određenim u zimu ili proleće. Uprkos prihvatljivoj nepreciznosti i netačnosti obe ispitivane imunohemijske metode, dobijeni rezultati nisu u zadovoljavajućoj korelaciji sa HPLC-UV metodom (r lt 0,9), koja je korišćena kao referentna u ovom slučaju. Uprkos ovoj činjenici, metode su pokazale zadovoljavajuću sposobnost klasifikacije pacijenata u kategorije statusa vitamina D, što je važno za dijagnozu deficijencije vitamina D i praćenje terapije. Oko dve trećine (68,5%) ispitivane populacije je imalo deficijenciju vitamina D (25(OH)D lt 50 nmol/L) i samo 8% je imalo preporučenu koncentraciju 25(OH)D (>75 nmol/L).",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience, Poređenje tri različite metode za određivanje 25(OH)-vitamina D i statusa vitamina D u opštoj populaciji - srpsko iskustvo",
volume = "31",
number = "4",
pages = "347-357",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1656"
}

Jovičić, S., Ignjatović, S., Kangrga, R., Beletić, A., Mirković, D.,& Majkić-Singh, N.. (2012). Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 31(4), 347-357.
https://hdl.handle.net/21.15107/rcub_farfar_1656

Jovičić S, Ignjatović S, Kangrga R, Beletić A, Mirković D, Majkić-Singh N. Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience. in Journal of Medical Biochemistry. 2012;31(4):347-357.
https://hdl.handle.net/21.15107/rcub_farfar_1656 .

Jovičić, Snežana, Ignjatović, Svetlana, Kangrga, Ranka, Beletić, Anđelo, Mirković, Duško, Majkić-Singh, Nada, "Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience" in Journal of Medical Biochemistry, 31, no. 4 (2012):347-357,
https://hdl.handle.net/21.15107/rcub_farfar_1656 .

TY  - JOUR
AU  - Dajak, Marijana
AU  - Bontić, Ana
AU  - Ignjatović, Svetlana
AU  - Pavlović, Jelena
AU  - Majkić-Singh, Nada
AU  - Lezaić, Višnja
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1747
AB  - Introduction One of the criteria for chronic kidney disease detection is determination of microalbuminuria. Objective This analysis was performed to evaluate accuracy of three useful methods for microalbuminuria detection in 24h urine collection and in the morning urine specimen calculated from urine albumin creatinine ratio, or with a dipstick in patients with different kidney diseases or kidney function. Methods Microalbuminuria was detected in 74 patients referred to the Outpatient Nephrology Department for kidney function determination or regular nephrology checking. Albumin concentration determined using immunonephelometry was lower than 300 mg/day. Discriminates cutoff values for spot urine test strip and albumin creatinin ratio in predicting 24 h protein 'threshold' excretion were determined using ROC analysis. Results Mean value of 24 h microalbuminuria was 80.3 mg/24 h, and value >30 mg/24 h was present in 71.8% of patient. Correlation coefficients between dipstick microalbuminuria or albumin/creatinine ratio in a spot urine specimen and 24 h microalbuminuria were 0.709 and 0.598 (p lt 0.0001). For pathological value of 24 h microalbuminuria >30 mg/24 h, the coresponding dipstick microalbuminuria value was >= 20 mg/L (AUC 0.849, specificity 95%, positive predictive value 97.3%), and >= 3.55 mg albumin/mmol creatinine ratio (AUC 0.914, specificity 90% and positive predictive value 95.5%). No difference was found between dipstick mikroalbuminuria and albumin/creatinine ratio value. In addition, albumin/creatinine ratio value from 24 h urine was similar to the value obtained from the spot urine sample. Conclusion Obtained results indicated that albuminuria could be determined accurately in spot urine either with the Micral test strip or with albumin creatinine ratio.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Evaluation of Methods for Rapid Microalbuminuria Screening in Kidney Diseased Patients
VL  - 140
IS  - 3-4
SP  - 173
EP  - 178
DO  - 10.2298/SARH1204173D
ER  - 

@article{
author = "Dajak, Marijana and Bontić, Ana and Ignjatović, Svetlana and Pavlović, Jelena and Majkić-Singh, Nada and Lezaić, Višnja",
year = "2012",
abstract = "Introduction One of the criteria for chronic kidney disease detection is determination of microalbuminuria. Objective This analysis was performed to evaluate accuracy of three useful methods for microalbuminuria detection in 24h urine collection and in the morning urine specimen calculated from urine albumin creatinine ratio, or with a dipstick in patients with different kidney diseases or kidney function. Methods Microalbuminuria was detected in 74 patients referred to the Outpatient Nephrology Department for kidney function determination or regular nephrology checking. Albumin concentration determined using immunonephelometry was lower than 300 mg/day. Discriminates cutoff values for spot urine test strip and albumin creatinin ratio in predicting 24 h protein 'threshold' excretion were determined using ROC analysis. Results Mean value of 24 h microalbuminuria was 80.3 mg/24 h, and value >30 mg/24 h was present in 71.8% of patient. Correlation coefficients between dipstick microalbuminuria or albumin/creatinine ratio in a spot urine specimen and 24 h microalbuminuria were 0.709 and 0.598 (p lt 0.0001). For pathological value of 24 h microalbuminuria >30 mg/24 h, the coresponding dipstick microalbuminuria value was >= 20 mg/L (AUC 0.849, specificity 95%, positive predictive value 97.3%), and >= 3.55 mg albumin/mmol creatinine ratio (AUC 0.914, specificity 90% and positive predictive value 95.5%). No difference was found between dipstick mikroalbuminuria and albumin/creatinine ratio value. In addition, albumin/creatinine ratio value from 24 h urine was similar to the value obtained from the spot urine sample. Conclusion Obtained results indicated that albuminuria could be determined accurately in spot urine either with the Micral test strip or with albumin creatinine ratio.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Evaluation of Methods for Rapid Microalbuminuria Screening in Kidney Diseased Patients",
volume = "140",
number = "3-4",
pages = "173-178",
doi = "10.2298/SARH1204173D"
}

Dajak, M., Bontić, A., Ignjatović, S., Pavlović, J., Majkić-Singh, N.,& Lezaić, V.. (2012). Evaluation of Methods for Rapid Microalbuminuria Screening in Kidney Diseased Patients. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 140(3-4), 173-178.
https://doi.org/10.2298/SARH1204173D

Dajak M, Bontić A, Ignjatović S, Pavlović J, Majkić-Singh N, Lezaić V. Evaluation of Methods for Rapid Microalbuminuria Screening in Kidney Diseased Patients. in Srpski arhiv za celokupno lekarstvo. 2012;140(3-4):173-178.
doi:10.2298/SARH1204173D .

Dajak, Marijana, Bontić, Ana, Ignjatović, Svetlana, Pavlović, Jelena, Majkić-Singh, Nada, Lezaić, Višnja, "Evaluation of Methods for Rapid Microalbuminuria Screening in Kidney Diseased Patients" in Srpski arhiv za celokupno lekarstvo, 140, no. 3-4 (2012):173-178,
https://doi.org/10.2298/SARH1204173D . .

TY  - JOUR
AU  - Jovičić, Snežana
AU  - Ignjatović, Svetlana
AU  - Majkić-Singh, Nada
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1667
AB  - Vitamin D is not technically a vitamin, since it is not an essential dietary factor. It is rather a prohormone produced photochemically in the skin from 7-dehydrocholesterol. Vitamin D and its metabolites may be categorized as either cholecalciferols or ergocalciferols. Cholecalciferol (vitamin D3) is the parent compound of the naturally occurring family and is produced in the skin from 7-dehydrocholesterol on exposure to the ultraviolet B portion of sunlight. Vitamin D2 (ergocalciferol), the parent compound of the other family, is manufactured by irradiation of ergosterol produced by yeasts and its potency is less than one-third of vitamin D3's potency. The steps in the vitamin D endocrine system include the following: 1) the photoconversion of 7-dehydrocholesterol to vitamin D3 in the skin or dietary intake of vitamin D3; 2) metabolism of vitamin D3 by the liver to 25-hydroxyvitamin-D3 [25(OH)D3], the major form of vitamin D circulating in the blood compartment; 3) conversion of 25(OH)D3 by the kidney (functioning as an endocrine gland) to the hormone 1,25-dihydroxyvitamin D3 [1,25(OH)2D3 ]; 4) systemic transport of the dihydroxylated metabolite 1,25(OH)2D3 to distal target organs; and 5) binding of 1,25(OH)2D3 to a nuclear receptor (VDR) at target organs, followed by generation of appropriate biological responses. The activation of vitamin D to its hormonal form is mediated by cytochrome P450 enzymes. Six cytochrome P450 (CYP) isoforms have been shown to hydroxylate vitamin D. Four of these, CYP27A1, CYP2R1, CYP3A4 and CYP2J3, are candidates for the enzyme vitamin D 25-hydroxylase that is involved in the first step of activation. The highly regulated, renal enzyme 25-hydroxyvitamin D-1a-hydro xylase contains the component CYP27B1, which completes the activation pathway to the hormonal form 1,25(OH)2D3. A five-step inactivation pathway from 1,25(OH)2D3 to calcitroic acid is attributed to a single multifunctional CYP, CYP24A1, which is transcriptionally induced in vitamin D target cells by the action of 1,25(OH)2D3. An additional key component in the operation of the vitamin D endocrine system is the plasma vitamin D binding protein (DBP), which carries vitamin D3 and its metabolites to their metabolism and target organs. DBP is a specific, high-affinity transport protein. It is synthesized by the liver and circulates in great excess, with fewer than 5% of the binding sites normally occupied. 1,25(OH)2D3, acts as a ligand for a nuclear transcription factor, vitamin D receptor - VDR, which like all other nuclear receptors, regulates gene transcription and cell function. The widespread presence of VDR, and the key activating (1a-hydroxylase, CYP27B1) and inactivating (24-hydroxylase, CYP24A1) enzymes in most mammalian cells means that the cells in these tissues have the potential to produce biological responses, depending on the availability of appropriate amounts of vitamin D3. Thanks to this widespread presence of elements of vitamin D endocrine system, its biological features are being recognized outside bone tissue, i.e. calcium and phosphate metabolism.
AB  - Vitamin D nije pravi vitamin, odnosno nije esencijalni dijetetski faktor, već je pre prohormon koji nastaje fotohemijskom reakcijom u koži iz 7-dehidroholesterola. Vita min D i njegovi metaboliti mogu da se kategorizuju kao holekalciferoli ili ergokalciferoli. Holekalciferol (vitamin D3) je polazno jedinjenje za familiju koja se nalazi u prirodi i produkuje se u koži iz 7-dehidroholesterola pri izlaganju ultraljubičastom B delu spektra sunčeve svetlosti. Vitamin D2 (ergokalciferol), polazno jedinjenje druge familije, nastaje radijacijom ergosterola koga produkuju kvasci i ima samo jednu trećinu aktivnosti vitamina D3. Faze u endokrinom sistemu vitamina D su: 1) fotokonverzija 7-dehidroholesterola u vitamin D3 u koži ili unos vitamina D3-hranom; 2) metabolizam vitamina D3 u jetri do 25-hidroksivitamina D3 [25(OH)D3], glavnog oblika vitamina D u cirkulaciji; 3) konverzija 25(OH)D3 u bubregu (koji ovde funkcioniše kao endokrina žlezda) do hormona 1,25-dihidroksivitamin D3 [1,25(OH)2D3]; 4) sistemski transport dihidroksi-metabolita do distalnih ciljnih organa; i 5) vezivanje 1,25(OH)2D3 za nuklearni receptor (VDR) u ciljnim organima, što prati odgovarajući biološki odgovor. Aktivacija vitamina D do hormonskog oblika je posredovana citohrom P450 enzimima. Pokazano je da šest izoformi citohroma P450 (CYP) učestvuje u hidroksilaciji vitamina D. Za četiri od njih, CYP27A1, CYP2R1, CYP3A4 i CYP2J3, se pretpostavlja da imaju aktivnost 25-hidroksilaze koja uče s tvuje u prvom koraku aktivacije. Renalni enzim, 25-hidroksivitamin D-1a-hidroksilaza sa strogo regulisanom aktivnošću, predstavlja CYP27B1, koji završava aktivaciju do hormonskog oblika 1,25(OH)2D3. Proces inaktivacije, koji se sastoji iz pet stupnjeva od 1,25(OH)2D3 do kalcitroične kiseline, obavlja jedan multifunkcionalni CYP, CYP24A1, čija je transkripcija indukovana u ciljnim ćelija carbonma dejstva vitamina D posredstvom 1,25(OH)2D3. Dodatna ključna komponenta u dejstvu vitamin D endokrinog sistema je vitamin D vezujući protein u plazmi (DBP), koji transportuje vitamin D3 i njegove metabolite do ciljnih i organa gde se odvija njihov metabolizam. DBP je specifičan transportni protein velikog afiniteta. Sintetiše se u jetri i cirkuliše u velikom višku, sa zasićenjem vezujućih mesta manjim od 5%. 1,25(OH)2D3 deluje kao ligand nuklearnog transkripcionog faktora, VDR, koji reguliše transkripciju gena i funkciju ćelija. široka rasprostranjenost VDR i ključnih enizma aktivacije (1a-hidroksilaza, CYP27B1) i inaktivacije (24-hidroksilaza, CYP24A1) u većini ćelija sisara znači da ćelije u ovim tkivima imaju potencijal za produkovanje bioloških odgovora, zavisno od raspoloživosti dovoljnih ko li čina vitamina D3. Zahvaljujući rasprostranjenosti elemenata endokrinog sistema vitamina D, njegove biološke oso bi ne se prepoznaju i izvan koštanog sistema, odnosno metabolizma kalcijuma i fosfora.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Biochemistry and metabolism of vitamin D
T1  - Biohemija i metabolizam vitamina D
VL  - 31
IS  - 4
SP  - 309
EP  - 315
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1667
ER  - 

@article{
author = "Jovičić, Snežana and Ignjatović, Svetlana and Majkić-Singh, Nada",
year = "2012",
abstract = "Vitamin D is not technically a vitamin, since it is not an essential dietary factor. It is rather a prohormone produced photochemically in the skin from 7-dehydrocholesterol. Vitamin D and its metabolites may be categorized as either cholecalciferols or ergocalciferols. Cholecalciferol (vitamin D3) is the parent compound of the naturally occurring family and is produced in the skin from 7-dehydrocholesterol on exposure to the ultraviolet B portion of sunlight. Vitamin D2 (ergocalciferol), the parent compound of the other family, is manufactured by irradiation of ergosterol produced by yeasts and its potency is less than one-third of vitamin D3's potency. The steps in the vitamin D endocrine system include the following: 1) the photoconversion of 7-dehydrocholesterol to vitamin D3 in the skin or dietary intake of vitamin D3; 2) metabolism of vitamin D3 by the liver to 25-hydroxyvitamin-D3 [25(OH)D3], the major form of vitamin D circulating in the blood compartment; 3) conversion of 25(OH)D3 by the kidney (functioning as an endocrine gland) to the hormone 1,25-dihydroxyvitamin D3 [1,25(OH)2D3 ]; 4) systemic transport of the dihydroxylated metabolite 1,25(OH)2D3 to distal target organs; and 5) binding of 1,25(OH)2D3 to a nuclear receptor (VDR) at target organs, followed by generation of appropriate biological responses. The activation of vitamin D to its hormonal form is mediated by cytochrome P450 enzymes. Six cytochrome P450 (CYP) isoforms have been shown to hydroxylate vitamin D. Four of these, CYP27A1, CYP2R1, CYP3A4 and CYP2J3, are candidates for the enzyme vitamin D 25-hydroxylase that is involved in the first step of activation. The highly regulated, renal enzyme 25-hydroxyvitamin D-1a-hydro xylase contains the component CYP27B1, which completes the activation pathway to the hormonal form 1,25(OH)2D3. A five-step inactivation pathway from 1,25(OH)2D3 to calcitroic acid is attributed to a single multifunctional CYP, CYP24A1, which is transcriptionally induced in vitamin D target cells by the action of 1,25(OH)2D3. An additional key component in the operation of the vitamin D endocrine system is the plasma vitamin D binding protein (DBP), which carries vitamin D3 and its metabolites to their metabolism and target organs. DBP is a specific, high-affinity transport protein. It is synthesized by the liver and circulates in great excess, with fewer than 5% of the binding sites normally occupied. 1,25(OH)2D3, acts as a ligand for a nuclear transcription factor, vitamin D receptor - VDR, which like all other nuclear receptors, regulates gene transcription and cell function. The widespread presence of VDR, and the key activating (1a-hydroxylase, CYP27B1) and inactivating (24-hydroxylase, CYP24A1) enzymes in most mammalian cells means that the cells in these tissues have the potential to produce biological responses, depending on the availability of appropriate amounts of vitamin D3. Thanks to this widespread presence of elements of vitamin D endocrine system, its biological features are being recognized outside bone tissue, i.e. calcium and phosphate metabolism., Vitamin D nije pravi vitamin, odnosno nije esencijalni dijetetski faktor, već je pre prohormon koji nastaje fotohemijskom reakcijom u koži iz 7-dehidroholesterola. Vita min D i njegovi metaboliti mogu da se kategorizuju kao holekalciferoli ili ergokalciferoli. Holekalciferol (vitamin D3) je polazno jedinjenje za familiju koja se nalazi u prirodi i produkuje se u koži iz 7-dehidroholesterola pri izlaganju ultraljubičastom B delu spektra sunčeve svetlosti. Vitamin D2 (ergokalciferol), polazno jedinjenje druge familije, nastaje radijacijom ergosterola koga produkuju kvasci i ima samo jednu trećinu aktivnosti vitamina D3. Faze u endokrinom sistemu vitamina D su: 1) fotokonverzija 7-dehidroholesterola u vitamin D3 u koži ili unos vitamina D3-hranom; 2) metabolizam vitamina D3 u jetri do 25-hidroksivitamina D3 [25(OH)D3], glavnog oblika vitamina D u cirkulaciji; 3) konverzija 25(OH)D3 u bubregu (koji ovde funkcioniše kao endokrina žlezda) do hormona 1,25-dihidroksivitamin D3 [1,25(OH)2D3]; 4) sistemski transport dihidroksi-metabolita do distalnih ciljnih organa; i 5) vezivanje 1,25(OH)2D3 za nuklearni receptor (VDR) u ciljnim organima, što prati odgovarajući biološki odgovor. Aktivacija vitamina D do hormonskog oblika je posredovana citohrom P450 enzimima. Pokazano je da šest izoformi citohroma P450 (CYP) učestvuje u hidroksilaciji vitamina D. Za četiri od njih, CYP27A1, CYP2R1, CYP3A4 i CYP2J3, se pretpostavlja da imaju aktivnost 25-hidroksilaze koja uče s tvuje u prvom koraku aktivacije. Renalni enzim, 25-hidroksivitamin D-1a-hidroksilaza sa strogo regulisanom aktivnošću, predstavlja CYP27B1, koji završava aktivaciju do hormonskog oblika 1,25(OH)2D3. Proces inaktivacije, koji se sastoji iz pet stupnjeva od 1,25(OH)2D3 do kalcitroične kiseline, obavlja jedan multifunkcionalni CYP, CYP24A1, čija je transkripcija indukovana u ciljnim ćelija carbonma dejstva vitamina D posredstvom 1,25(OH)2D3. Dodatna ključna komponenta u dejstvu vitamin D endokrinog sistema je vitamin D vezujući protein u plazmi (DBP), koji transportuje vitamin D3 i njegove metabolite do ciljnih i organa gde se odvija njihov metabolizam. DBP je specifičan transportni protein velikog afiniteta. Sintetiše se u jetri i cirkuliše u velikom višku, sa zasićenjem vezujućih mesta manjim od 5%. 1,25(OH)2D3 deluje kao ligand nuklearnog transkripcionog faktora, VDR, koji reguliše transkripciju gena i funkciju ćelija. široka rasprostranjenost VDR i ključnih enizma aktivacije (1a-hidroksilaza, CYP27B1) i inaktivacije (24-hidroksilaza, CYP24A1) u većini ćelija sisara znači da ćelije u ovim tkivima imaju potencijal za produkovanje bioloških odgovora, zavisno od raspoloživosti dovoljnih ko li čina vitamina D3. Zahvaljujući rasprostranjenosti elemenata endokrinog sistema vitamina D, njegove biološke oso bi ne se prepoznaju i izvan koštanog sistema, odnosno metabolizma kalcijuma i fosfora.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Biochemistry and metabolism of vitamin D, Biohemija i metabolizam vitamina D",
volume = "31",
number = "4",
pages = "309-315",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1667"
}

Jovičić, S., Ignjatović, S.,& Majkić-Singh, N.. (2012). Biochemistry and metabolism of vitamin D. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 31(4), 309-315.
https://hdl.handle.net/21.15107/rcub_farfar_1667

Jovičić S, Ignjatović S, Majkić-Singh N. Biochemistry and metabolism of vitamin D. in Journal of Medical Biochemistry. 2012;31(4):309-315.
https://hdl.handle.net/21.15107/rcub_farfar_1667 .

Jovičić, Snežana, Ignjatović, Svetlana, Majkić-Singh, Nada, "Biochemistry and metabolism of vitamin D" in Journal of Medical Biochemistry, 31, no. 4 (2012):309-315,
https://hdl.handle.net/21.15107/rcub_farfar_1667 .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Seferović, Jelena
AU  - Ignjatović, Svetlana
AU  - Singh, Sandra
AU  - Majkić-Singh, Nada
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1756
AB  - The importance of the association of antiphospholipid antibodies (aPL Abs) with the features of type II diabetes mellitus has not yet been elucidated. The aim of this work was to investigate the association of aPL Abs with adiponectin and non-esterified fatty acids (NEFA) in type II diabetes mellitus patients without micro and/or macrovascular complications, and to analyze the differences between the male and female patients with regard to the abovementioned parameters. Male patients with type II diabetes mellitus showed a positive correlation between NEFA concentrations and anti-oxLDL antibodies (r = 0.334, p = 0.019). A weak, but statistically significant correlation between adiponectin concentrations and the IgM isotype of anti-annexin A5 antibodies was found in type II diabetes mellitus patients (r = 0.285, p = 0.011). The presence of a positive correlation between NEFA and anti-oxLDL antibodies might be useful in the detection of patients with premature atherosclerosis in type II diabetes mellitus patients without any micro and/or macrovascular complications among type II diabetes mellitus patients.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Adiponectin, non-esterified fatty acids and antiphospholipid antibodies in type II diabetes mellitus
VL  - 31
IS  - 3
SP  - 199
EP  - 204
DO  - 10.2478/v10011-012-0009-y
ER  - 

@article{
author = "Becarević, Mirjana and Seferović, Jelena and Ignjatović, Svetlana and Singh, Sandra and Majkić-Singh, Nada",
year = "2012",
abstract = "The importance of the association of antiphospholipid antibodies (aPL Abs) with the features of type II diabetes mellitus has not yet been elucidated. The aim of this work was to investigate the association of aPL Abs with adiponectin and non-esterified fatty acids (NEFA) in type II diabetes mellitus patients without micro and/or macrovascular complications, and to analyze the differences between the male and female patients with regard to the abovementioned parameters. Male patients with type II diabetes mellitus showed a positive correlation between NEFA concentrations and anti-oxLDL antibodies (r = 0.334, p = 0.019). A weak, but statistically significant correlation between adiponectin concentrations and the IgM isotype of anti-annexin A5 antibodies was found in type II diabetes mellitus patients (r = 0.285, p = 0.011). The presence of a positive correlation between NEFA and anti-oxLDL antibodies might be useful in the detection of patients with premature atherosclerosis in type II diabetes mellitus patients without any micro and/or macrovascular complications among type II diabetes mellitus patients.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Adiponectin, non-esterified fatty acids and antiphospholipid antibodies in type II diabetes mellitus",
volume = "31",
number = "3",
pages = "199-204",
doi = "10.2478/v10011-012-0009-y"
}

Becarević, M., Seferović, J., Ignjatović, S., Singh, S.,& Majkić-Singh, N.. (2012). Adiponectin, non-esterified fatty acids and antiphospholipid antibodies in type II diabetes mellitus. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 31(3), 199-204.
https://doi.org/10.2478/v10011-012-0009-y

Becarević M, Seferović J, Ignjatović S, Singh S, Majkić-Singh N. Adiponectin, non-esterified fatty acids and antiphospholipid antibodies in type II diabetes mellitus. in Journal of Medical Biochemistry. 2012;31(3):199-204.
doi:10.2478/v10011-012-0009-y .

Becarević, Mirjana, Seferović, Jelena, Ignjatović, Svetlana, Singh, Sandra, Majkić-Singh, Nada, "Adiponectin, non-esterified fatty acids and antiphospholipid antibodies in type II diabetes mellitus" in Journal of Medical Biochemistry, 31, no. 3 (2012):199-204,
https://doi.org/10.2478/v10011-012-0009-y . .

TY  - JOUR
AU  - Mitrović, Mirjana
AU  - Šumarac, Zorica
AU  - Antić, Darko
AU  - Bogdanović, Andrija
AU  - Elezović, Ivo
AU  - Vukosavljević, Dragana
AU  - Ignjatović, Svetlana
AU  - Majkić-Singh, Nada
AU  - Suvajdzić, Nada
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1754
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Blood Cells Molecules and Diseases
T1  - Markers of coagulation activation and enhanced fibrinolysis in Gaucher type 1 patient: Effects of enzyme replacement therapy
VL  - 49
IS  - 1
SP  - 58
EP  - 59
DO  - 10.1016/j.bcmd.2012.03.003
ER  - 

@article{
author = "Mitrović, Mirjana and Šumarac, Zorica and Antić, Darko and Bogdanović, Andrija and Elezović, Ivo and Vukosavljević, Dragana and Ignjatović, Svetlana and Majkić-Singh, Nada and Suvajdzić, Nada",
year = "2012",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Blood Cells Molecules and Diseases",
title = "Markers of coagulation activation and enhanced fibrinolysis in Gaucher type 1 patient: Effects of enzyme replacement therapy",
volume = "49",
number = "1",
pages = "58-59",
doi = "10.1016/j.bcmd.2012.03.003"
}

Mitrović, M., Šumarac, Z., Antić, D., Bogdanović, A., Elezović, I., Vukosavljević, D., Ignjatović, S., Majkić-Singh, N.,& Suvajdzić, N.. (2012). Markers of coagulation activation and enhanced fibrinolysis in Gaucher type 1 patient: Effects of enzyme replacement therapy. in Blood Cells Molecules and Diseases
Academic Press Inc Elsevier Science, San Diego., 49(1), 58-59.
https://doi.org/10.1016/j.bcmd.2012.03.003

Mitrović M, Šumarac Z, Antić D, Bogdanović A, Elezović I, Vukosavljević D, Ignjatović S, Majkić-Singh N, Suvajdzić N. Markers of coagulation activation and enhanced fibrinolysis in Gaucher type 1 patient: Effects of enzyme replacement therapy. in Blood Cells Molecules and Diseases. 2012;49(1):58-59.
doi:10.1016/j.bcmd.2012.03.003 .

Mitrović, Mirjana, Šumarac, Zorica, Antić, Darko, Bogdanović, Andrija, Elezović, Ivo, Vukosavljević, Dragana, Ignjatović, Svetlana, Majkić-Singh, Nada, Suvajdzić, Nada, "Markers of coagulation activation and enhanced fibrinolysis in Gaucher type 1 patient: Effects of enzyme replacement therapy" in Blood Cells Molecules and Diseases, 49, no. 1 (2012):58-59,
https://doi.org/10.1016/j.bcmd.2012.03.003 . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Ignjatović, Svetlana
AU  - Majkić-Singh, Nada
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1632
AB  - Background: To investigate the association between clinical and serological features of patients with primary antiphospholipid syndrome (PAPS) and TNF-alpha, interleukin 6 (IL-6), and soluble IL-2 receptor (sIL-2R). Methods: ELISA was used for measurement of antibodies (Abs) and TNF-alpha, while IL-6 and sIL-2R were measured by chemiluminescence. Results: PAPS patients with pulmonary emboli showed positive correlation between IgM isotype of anti-annexin A5 antibodies and TNF-alpha (r = 0.894, p = 0.041) and IgM class of anticardiolipin antibodies and sIL-2R (r = 0.900, p = 0.037). In PAPS with cerebrovascular insults, positive correlation was noticed between TNF-alpha and IgG isotype of anticardiolipin (r = 0.624, p = 0.040) and anti-annexinA5 Abs (r = 0.768, p = 0.006). Conclusions: Isotype analysis of antiphospholipid and anti-annexin A5 Abs and investigation of their association with TNF-alpha is important for differentiation of PAPS patients that are prone to further deterioration of arterial and venous thromboses. (Clin. Lab. 2012;58:1079-1084. DOI: 10.7754/Clin.Lab.2012.110918)
PB  - Clin Lab Publ, Heidelberg
T2  - Clinical Laboratory
T1  - Deterioration of Thromboses in Primary Antiphospholipid Syndrome: TNF-alpha and Anti-Annexin A5 Antibodies
VL  - 58
IS  - 9-10
SP  - 1079
EP  - 1084
DO  - 10.7754/Clin.Lab.2012.110918
ER  - 

@article{
author = "Becarević, Mirjana and Ignjatović, Svetlana and Majkić-Singh, Nada",
year = "2012",
abstract = "Background: To investigate the association between clinical and serological features of patients with primary antiphospholipid syndrome (PAPS) and TNF-alpha, interleukin 6 (IL-6), and soluble IL-2 receptor (sIL-2R). Methods: ELISA was used for measurement of antibodies (Abs) and TNF-alpha, while IL-6 and sIL-2R were measured by chemiluminescence. Results: PAPS patients with pulmonary emboli showed positive correlation between IgM isotype of anti-annexin A5 antibodies and TNF-alpha (r = 0.894, p = 0.041) and IgM class of anticardiolipin antibodies and sIL-2R (r = 0.900, p = 0.037). In PAPS with cerebrovascular insults, positive correlation was noticed between TNF-alpha and IgG isotype of anticardiolipin (r = 0.624, p = 0.040) and anti-annexinA5 Abs (r = 0.768, p = 0.006). Conclusions: Isotype analysis of antiphospholipid and anti-annexin A5 Abs and investigation of their association with TNF-alpha is important for differentiation of PAPS patients that are prone to further deterioration of arterial and venous thromboses. (Clin. Lab. 2012;58:1079-1084. DOI: 10.7754/Clin.Lab.2012.110918)",
publisher = "Clin Lab Publ, Heidelberg",
journal = "Clinical Laboratory",
title = "Deterioration of Thromboses in Primary Antiphospholipid Syndrome: TNF-alpha and Anti-Annexin A5 Antibodies",
volume = "58",
number = "9-10",
pages = "1079-1084",
doi = "10.7754/Clin.Lab.2012.110918"
}

Becarević, M., Ignjatović, S.,& Majkić-Singh, N.. (2012). Deterioration of Thromboses in Primary Antiphospholipid Syndrome: TNF-alpha and Anti-Annexin A5 Antibodies. in Clinical Laboratory
Clin Lab Publ, Heidelberg., 58(9-10), 1079-1084.
https://doi.org/10.7754/Clin.Lab.2012.110918

Becarević M, Ignjatović S, Majkić-Singh N. Deterioration of Thromboses in Primary Antiphospholipid Syndrome: TNF-alpha and Anti-Annexin A5 Antibodies. in Clinical Laboratory. 2012;58(9-10):1079-1084.
doi:10.7754/Clin.Lab.2012.110918 .

Becarević, Mirjana, Ignjatović, Svetlana, Majkić-Singh, Nada, "Deterioration of Thromboses in Primary Antiphospholipid Syndrome: TNF-alpha and Anti-Annexin A5 Antibodies" in Clinical Laboratory, 58, no. 9-10 (2012):1079-1084,
https://doi.org/10.7754/Clin.Lab.2012.110918 . .

TY  - CONF
AU  - Beletić, Anđelo
AU  - Đorđević, Valentina
AU  - Canić, I.
AU  - Kocica, T.
AU  - Kuzmanović, I.
AU  - Golubović, Milka
AU  - Mirković, Duško
AU  - Radojković, Dragica
AU  - Majkić-Singh, Nada
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1531
PB  - Walter de Gruyter & Co, Berlin
C3  - Clinical Chemistry and Laboratory Medicine
T1  - Experiences in simultaneous detection of factor v leiden, factor ii g20210a, mthfr c677t and mthfr a1298c mutations in patients with thrombophilia
VL  - 49
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1531
ER  - 

@conference{
author = "Beletić, Anđelo and Đorđević, Valentina and Canić, I. and Kocica, T. and Kuzmanović, I. and Golubović, Milka and Mirković, Duško and Radojković, Dragica and Majkić-Singh, Nada",
year = "2011",
publisher = "Walter de Gruyter & Co, Berlin",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Experiences in simultaneous detection of factor v leiden, factor ii g20210a, mthfr c677t and mthfr a1298c mutations in patients with thrombophilia",
volume = "49",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1531"
}

Beletić, A., Đorđević, V., Canić, I., Kocica, T., Kuzmanović, I., Golubović, M., Mirković, D., Radojković, D.,& Majkić-Singh, N.. (2011). Experiences in simultaneous detection of factor v leiden, factor ii g20210a, mthfr c677t and mthfr a1298c mutations in patients with thrombophilia. in Clinical Chemistry and Laboratory Medicine
Walter de Gruyter & Co, Berlin., 49.
https://hdl.handle.net/21.15107/rcub_farfar_1531

Beletić A, Đorđević V, Canić I, Kocica T, Kuzmanović I, Golubović M, Mirković D, Radojković D, Majkić-Singh N. Experiences in simultaneous detection of factor v leiden, factor ii g20210a, mthfr c677t and mthfr a1298c mutations in patients with thrombophilia. in Clinical Chemistry and Laboratory Medicine. 2011;49.
https://hdl.handle.net/21.15107/rcub_farfar_1531 .

Beletić, Anđelo, Đorđević, Valentina, Canić, I., Kocica, T., Kuzmanović, I., Golubović, Milka, Mirković, Duško, Radojković, Dragica, Majkić-Singh, Nada, "Experiences in simultaneous detection of factor v leiden, factor ii g20210a, mthfr c677t and mthfr a1298c mutations in patients with thrombophilia" in Clinical Chemistry and Laboratory Medicine, 49 (2011),
https://hdl.handle.net/21.15107/rcub_farfar_1531 .

TY  - CONF
AU  - Becarević, Mirjana
AU  - Seferović, Jelena
AU  - Ignjatović, Svetlana
AU  - Majkić-Singh, Nada
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1495
PB  - Elsevier Ireland Ltd, Clare
C3  - Atherosclerosis Supplements
T1  - Association of tnf-alpha and anticardiolipin antibodies in type ii diabetes mellitus
VL  - 12
IS  - 1
SP  - 91
EP  - 91
DO  - 10.1016/S1567-5688(11)70427-5
ER  - 

@conference{
author = "Becarević, Mirjana and Seferović, Jelena and Ignjatović, Svetlana and Majkić-Singh, Nada",
year = "2011",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Atherosclerosis Supplements",
title = "Association of tnf-alpha and anticardiolipin antibodies in type ii diabetes mellitus",
volume = "12",
number = "1",
pages = "91-91",
doi = "10.1016/S1567-5688(11)70427-5"
}

Becarević, M., Seferović, J., Ignjatović, S.,& Majkić-Singh, N.. (2011). Association of tnf-alpha and anticardiolipin antibodies in type ii diabetes mellitus. in Atherosclerosis Supplements
Elsevier Ireland Ltd, Clare., 12(1), 91-91.
https://doi.org/10.1016/S1567-5688(11)70427-5

Becarević M, Seferović J, Ignjatović S, Majkić-Singh N. Association of tnf-alpha and anticardiolipin antibodies in type ii diabetes mellitus. in Atherosclerosis Supplements. 2011;12(1):91-91.
doi:10.1016/S1567-5688(11)70427-5 .

Becarević, Mirjana, Seferović, Jelena, Ignjatović, Svetlana, Majkić-Singh, Nada, "Association of tnf-alpha and anticardiolipin antibodies in type ii diabetes mellitus" in Atherosclerosis Supplements, 12, no. 1 (2011):91-91,
https://doi.org/10.1016/S1567-5688(11)70427-5 . .