@conference{
author = "Suručić, Relja and Drakul, Dragana and Kovačević, Nada and Kundaković-Vasović, Tatjana",
year = "2018",
abstract = "Terpeni predstavljaju veoma važnu grupu hemijskih jedinjenja kako zbog
industrijske upotrebe, tako i zbog svojih značajnih bioloških efekata koji se mogu
iskoristiti u medicini. Jedna su od najbrojnijih grupa sekundarnih metabolita biljaka, a
naziv ove klase jedinjenja potiče od terpentina (terpentinskog ulja), tečnog proizvoda
destilacije oleorezina bora. Terpinolen (p‐menta‐2,4(8)‐dien) je bezbojna ili bledo žuto
obojena tečnost aromatičnog mirisa. Ovaj monociklični, monoterpenski alken je široko
zastupljen u biljnim tkivima različitih četinara, paškanata, konoplje, čajevca, listu
kurkume i peršuna, a čest je sastojak etarskog ulja Citrus, Mentha, Juniperus i Myristica
vrsta. Jedna je od glavnih komponenata etarskog ulja nadzemnih delova endemične
vrste Seseli gracile Waldst. & Kit. (6,1‐57,5 %). Terpinolen poseduje potvrđenu
antiradikalsku, antihiperalgezijsku i antiedematoznu aktivnost. S obzirom da su neka
strukturno slična monoterpenska jedinjenja pokazala vazorelaksantnu aktivnost, cilj
ovog istraživanja je da se istraži vazorelaksantni potencijal terpinolena.
U ovom istraživanju ispitivan je efekat standarda terpinolena (43905 Sigma‐
Aldrich) na izolovanoj renalnoj arteriji pacova Wistar soja. Korišćeni su prstenasti
segmenti renalne arterije dužine 3‐5 mm sa očuvanim endotelom. Arterijski segmenti
su postavljani u vodeno kupatilo u Krebs‐Ringerov rastvor, na 37C i aerisani
kontinuiranim dovođenjem smješe 95 % O2 i 5 % CO2. Kontrakcija krvnih sudova je
izazvana primjenom fenilefrina (10‐6 M). Integritet endotela je potvrđen farmakološki,
primjenom acetilholina (10‐6 M). Na stabilan tonus krvnog suda izazvan fenilefrinom su
dodavane rastuće koncentracije terpinolena, pripremljenog od standarda
razblaživanjem u 5 % karboksi‐metil celuloze (0,2 % ‐ 33,3 %, kumulativno). Svaka
sledeća koncentracija je dodavana tek nakon što se završi efekat prethodno
primjenjene koncentracije, tj. otprilike nakon 15 minuta od prethodne. U kontrolnoj
seriji eksperimenata je na isti način dodavan rastvarač (6 dodavanja kumulativno, sa
vremenskim razmakom od 15 minuta).
Utvrđeno je da terpinolen u kocentraciji od 11,1% dovodi do statistički značajne
vazorelaksacije u odnosu na rastvarač kao kontrolu (F=2,584, p<0,05; Two‐way
ANOVA). Naši rezultati opravdavaju nastavak ispitivanja potencijalne primene
terpinolena u terapiji kardiovaskularnih oboljenja., Terpenes represent a very important group of chemical compounds because of
their industrial use, as well as medical use which is possible due to their significant
biological effects. They are one of the largest groups of secondary plant metabolites.
The name of this class of compounds comes from turpentine (oil of turpentine), a liquid
product of pine oleoresin distillation process.
Terpinolene (p‐menth‐2,4 (8)‐diene) is a colorless or pale yellow colored liquid
with aromatic odor. This monocyclic, monoterpenic alkene is widely represented in
plant tissues of various pines, pashkans, cannabis, tea tree, turmeric and parsley leaves,
and it is often a component of essential oils obtained from Citrus, Mentha, Juniperus and
Myristica species. It is one of the main components of the essential oil of aerial parts of
the endemic species Seseli gracile Waldst. & Kit. (6.1‐57.5%). Terpinolen possesses
confirmed antiradical, antihyperalgesic and antiedematous activity. Since some
structurally similar monoterpenic compounds have previously shown vasorelaxant
activity, the aim of this study was to investigate the vasorelaxant potential of
terpinolene.
In the present study, the effects of standard terpinolene (43905 Sigma‐Aldrich)
on the isolated renal artery of Wistar rats were investigated. Three to five mm long
arterial ring tissue segments with preserved endothelium were used. The arterial
segments were placed in water bath in the Krebs‐Ringer solution, at 37° C and
continuously aerated by 95% O2 and 5% CO2. Contractions of the blood vessels were
induced with 10‐6 M of phenylephrine. The endothelium integrity was confirmed
pharmacologically, by using the acetylcholine (10‐6M). After accomplishing tonic phase
of the contraction, increasing concentration of terpinolene (0.2 % ‐ 33.3 %,
cumulatively) in 5 % carboxymethyl cellulose (CMC) solution were added to the organ
bath. Each subsequent concentration was added only after the end of effect of the
previously applied concentration, i.e. approximately 15 minutes from the previous one.
In the control series of experiments, a solvent was added in the same manner.
It was found that terpinolene at the concentration of 11.1% exibits statistically
significant vasorelaxation in comparison to the solvent control (F = 2.584, p <0.05;
Two‐way ANOVA). Our results justify a continuation for the further studies of
terpinolene’s potential in the treatment of cardiovascular diseases.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Vazorelaksantna aktivnost terpinolena, Vasorelaxant activity of terpinolene",
volume = "68",
number = "3",
pages = "706-707",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5282"
}
