TY  - JOUR
AU  - Gkountanas, Kostas
AU  - Dagla, Ioanna
AU  - Gikas, Evangelos
AU  - Malenović, Anđelija
AU  - Dotsikas, Yannis
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4998
AB  - Chromatograms with overlapping peaks and a baseline rise or upset constitute a great challenge for analysts. Such a case regarding the analysis of bupropion hydrochloride and its 5 impurities in a tablet formulation was used as a model. A baseline correction technique for liquid chromatography coupled with diode array detection is described by using Rstudio. The asymmetry least squares (ALS) algorithm was used as implemented in the “baseline” package, with parameters lambda and p set to 4 and 0.05, respectively. Peak deconvolution and subsequent integration and area quantification were accomplished through Fytik software. Chromatographic data from the validation procedure were utilized to demonstrate the feasibility of the suggested method and whether this correction affects the outcome of the validation study. Finally, a robustness study was carried out in order to shed light on the factors that have a more significant influence on the baseline correction, showing the reliability of this procedure through random changes in its parameters.
PB  - MDPI
T2  - Analytica
T1  - Baseline Correction for HPLC Chromatograms by Using Free
Open-Source Software
VL  - 4
IS  - 1
SP  - 45
EP  - 53
DO  - 10.3390/analytica4010005
ER  - 

@article{
author = "Gkountanas, Kostas and Dagla, Ioanna and Gikas, Evangelos and Malenović, Anđelija and Dotsikas, Yannis",
year = "2023",
abstract = "Chromatograms with overlapping peaks and a baseline rise or upset constitute a great challenge for analysts. Such a case regarding the analysis of bupropion hydrochloride and its 5 impurities in a tablet formulation was used as a model. A baseline correction technique for liquid chromatography coupled with diode array detection is described by using Rstudio. The asymmetry least squares (ALS) algorithm was used as implemented in the “baseline” package, with parameters lambda and p set to 4 and 0.05, respectively. Peak deconvolution and subsequent integration and area quantification were accomplished through Fytik software. Chromatographic data from the validation procedure were utilized to demonstrate the feasibility of the suggested method and whether this correction affects the outcome of the validation study. Finally, a robustness study was carried out in order to shed light on the factors that have a more significant influence on the baseline correction, showing the reliability of this procedure through random changes in its parameters.",
publisher = "MDPI",
journal = "Analytica",
title = "Baseline Correction for HPLC Chromatograms by Using Free
Open-Source Software",
volume = "4",
number = "1",
pages = "45-53",
doi = "10.3390/analytica4010005"
}

Gkountanas, K., Dagla, I., Gikas, E., Malenović, A.,& Dotsikas, Y.. (2023). Baseline Correction for HPLC Chromatograms by Using Free
Open-Source Software. in Analytica
MDPI., 4(1), 45-53.
https://doi.org/10.3390/analytica4010005

Gkountanas K, Dagla I, Gikas E, Malenović A, Dotsikas Y. Baseline Correction for HPLC Chromatograms by Using Free
Open-Source Software. in Analytica. 2023;4(1):45-53.
doi:10.3390/analytica4010005 .

Gkountanas, Kostas, Dagla, Ioanna, Gikas, Evangelos, Malenović, Anđelija, Dotsikas, Yannis, "Baseline Correction for HPLC Chromatograms by Using Free
Open-Source Software" in Analytica, 4, no. 1 (2023):45-53,
https://doi.org/10.3390/analytica4010005 . .

TY  - JOUR
AU  - Gkountanas, Kostas
AU  - Malenović, Anđelija
AU  - Dotsikas, Yannis
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4312
AB  - A novel chaotropic chromatography method for the quantitative determination of bupropion and its impurities, following analytical quality-by-design (AQbD) principles, is presented. The analytical target profile (ATP) was defined on the basis of the efficient separation and reliable determination of bupropion and its five impurities in tablets. Preliminary experiments revealed the need for the addition of a gradient elution part. A screening fractional factorial experimental design was employed to select the critical method parameters (CMPs) and a Box–Behnken design (BBD) was utilized to investigate their influence on predefined critical method attributes (CMAs). In order to compute the design space (DS), where CMPs meet predefined acceptance limits with a high level of probability (π ≥ 85%), Monte Carlo simulations were performed. The working point selected from the DS corresponded to the following conditions: 37.5% acetonitrile at the start of the gradient program (up to 70% at the end of the gradient program), 45 mM of potassium hexafluorophosphate in the water phase, and the start of the linear gradient step in the gradient program at 10 min. The method was validated according to ICH guidelines and applied to the analysis of Wellbutrin® tablets containing bupropion hydrochloride.
PB  - MDPI
T2  - Pharmaceuticals
T1  - Determination of Bupropion and Its Impurities via a Chaotropic Chromatography Method Following Analytical Quality-by-Design Principles for Method Development
VL  - 15
IS  - 10
DO  - 10.3390/ph15101196
ER  - 

@article{
author = "Gkountanas, Kostas and Malenović, Anđelija and Dotsikas, Yannis",
year = "2022",
abstract = "A novel chaotropic chromatography method for the quantitative determination of bupropion and its impurities, following analytical quality-by-design (AQbD) principles, is presented. The analytical target profile (ATP) was defined on the basis of the efficient separation and reliable determination of bupropion and its five impurities in tablets. Preliminary experiments revealed the need for the addition of a gradient elution part. A screening fractional factorial experimental design was employed to select the critical method parameters (CMPs) and a Box–Behnken design (BBD) was utilized to investigate their influence on predefined critical method attributes (CMAs). In order to compute the design space (DS), where CMPs meet predefined acceptance limits with a high level of probability (π ≥ 85%), Monte Carlo simulations were performed. The working point selected from the DS corresponded to the following conditions: 37.5% acetonitrile at the start of the gradient program (up to 70% at the end of the gradient program), 45 mM of potassium hexafluorophosphate in the water phase, and the start of the linear gradient step in the gradient program at 10 min. The method was validated according to ICH guidelines and applied to the analysis of Wellbutrin® tablets containing bupropion hydrochloride.",
publisher = "MDPI",
journal = "Pharmaceuticals",
title = "Determination of Bupropion and Its Impurities via a Chaotropic Chromatography Method Following Analytical Quality-by-Design Principles for Method Development",
volume = "15",
number = "10",
doi = "10.3390/ph15101196"
}

Gkountanas, K., Malenović, A.,& Dotsikas, Y.. (2022). Determination of Bupropion and Its Impurities via a Chaotropic Chromatography Method Following Analytical Quality-by-Design Principles for Method Development. in Pharmaceuticals
MDPI., 15(10).
https://doi.org/10.3390/ph15101196

Gkountanas K, Malenović A, Dotsikas Y. Determination of Bupropion and Its Impurities via a Chaotropic Chromatography Method Following Analytical Quality-by-Design Principles for Method Development. in Pharmaceuticals. 2022;15(10).
doi:10.3390/ph15101196 .

Gkountanas, Kostas, Malenović, Anđelija, Dotsikas, Yannis, "Determination of Bupropion and Its Impurities via a Chaotropic Chromatography Method Following Analytical Quality-by-Design Principles for Method Development" in Pharmaceuticals, 15, no. 10 (2022),
https://doi.org/10.3390/ph15101196 . .