@conference{
author = "Bubić Pajić, Nataša and Ilić, Tanja and Nikolić, Ines and Vučen, Sonja and Dobričić, Vladimir and Savić, Snežana",
year = "2019",
abstract = "Efikasnost terapije gljivičnih infekcija kože zavisi od sposobnosti lijeka da premosti barijeru stratum
corneuma i dospije u vijabilni epidermis. S obzirom da su mnogi antifungalni lijekovi slabo rastvorljivi
u vodi i imaju nisku permeabilnost, razvoj pogodnih formulacija za efikasnu isporuku lijeka u kožu
nije jednostavan.
Cilj ovog rada je procjena efekta kombinovane primjene prethodno razvijenih biokompatibilnih
mikroemulzija i čvrstih silikonskih mikroigala na poboljšanu dermalnu isporuku antifungalnog lijeka
- sertakonazol-nitrata (SN).
Mikroemulzije su izrađivane dodatkom ulja (propilenglikol-monokaprilat) u smješu surfaktanta
i kosurfaktanta (gliceret-7-kaprilat/kaprat:etanol ili polisorbat 80: dietilenglikol monoetiletar)
uz miješanje u trajanju od 15 min. Nakon toga, odgovarajuća količina vode je postepeno dodana
i miješanje je vršeno dodatnih 15 min, nakon čega je u izrađenim uzorcima rastvoren SN (2%). In
vitro studije penetracije/permeacije sa ili bez predthodnog tretmana kože uha svinje ImmuPatch
silikonskim mikroiglama (Nacionalni Institut Tyndall, Kork, Irska) su sprovedene na Franz-ovim
difuzionim ćelijama.
Dobijeni rezultati su pokazali da je predtrtman kože mikroiglama značajno poboljšao isporuku SN
u koži iz obje ispitivane mikroemulzione formulacije. 24h nakon primjene uzoraka, količina lijeka
koja je deponovana u koži tretiranoj mikroiglama je bila veća 1,90-2,02 puta u odnosu na intaktnu
kožu. Međutim, količina lijeka koja je permeirala nakon 24h iz mikroemulzija kroz kožu tretiranu
mikroiglama je bila 26,96±7,88 μg/ml i 12,66±2,07 μg/ml, dok SN nije detektovan u akceptorskoj fazi
nakon primjene samih mikroemulzija.
Dobijeni rezultati ukazuju da bi kombinovana primjena mikroigala i biokompatibilnih mikroemulzija
mogla biti efikasan i bezbolan pristup za poboljšanu isporuku sertakonazol-nitrata u kožu. Međutim,
bez obzira na zanemarljivu permeaciju, odgovarajućim in vivo ispitivanjima na je potrebno potvrditi
odsustvo sistemskih neželjenih efekata., The effectiveness of a topically applied antifungal agent is directly related to its ability to pass the barrier of the stratum corneum and to reach at least the viable epidermis. Given that many antifungals are poorly soluble and have low skin permeability, development of a suitable formulation is not an easy task. The aim of our study was to evaluate the effect of the combination of previously designed seraconazole nitrate (SN) loaded biocompatible microemulsions with solid silicon microneedles for enhanced dermal drug delivery. For the preparation of MEs, oil (propylene glycol monocaprylate) was added to the mixture of surfactant and cosurfactant (glycereth-7-caprylate/caprate:ethanol or polysorbate 80: diethylene glycolmonoethyl ether) and mixed for 15 min. Then, proper amount of water was added and mixed for another 15 min. SN (2% w/w) was dissolved in previously prepared MEs. In vitro skin penetration/ permeation studies with and without skin pretreatment with ImmuPatch silicon microneedles (Tyndall National Institute, Cork, Ireland) were conducted on Franz-type diffusion cells using porcine ear skin. Our finding revealed that skin pretreatment with microneedles significantly improved SN delivery into the skin from both tested microemulsions, resulting in 1.90-2.02-fold enhanced level of the drug retained in the skin, as measured 24h after administration. However, the drug permeated 24h after application of tested formulations combined with microneedles was 26.96±7.88 μg/ml and 12.66±2.07 μg/ml, whereas SN did not permeated through the intact skin. The obtained results suggest that combination of solid microneedles and biocompatible microemulsions could be an effective and painless approach for enhancing drug delivery into the skin. However, despite negligible SN permeation, further in vivo study should be performed in order to confirm the absence of systemic adverse effects.",
publisher = "Prisma - korporativne komunikacije, Farmaceutska komora Crne Gore",
journal = "Treći kongres farmaceuta Crne Gore sa međunarodnim učešćem, 09 - 12. maj 2019. Budva, Bečići, Book of Abstracts",
title = "Kombinovana primjena silikonskih mikroigala i biokompatibilnih mikroemulzija za poboljšanu dermalnu isporuku sertakonazol-nitrata, Combined application of silicon microneedles and biocompatible microemulsions for improved dermal delivery of sertaconazole nitrate",
pages = "118-119",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5455"
}