TY  - JOUR
AU  - Radaković, Milena
AU  - Borozan, Sunčica
AU  - Đelić, Ninoslav
AU  - Ivanović, Sasa
AU  - Čupić-Miladinović, Dejana
AU  - Ristanić, Marko
AU  - Potparević, Biljana
AU  - Stanimirović, Zoran
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3064
AB  - This study is aimed at analysing biochemical and genetic endpoints of toxic effects after administration of adrenaline. For this purpose, the study was carried out on Wistar rats and three doses of adrenaline were used: 0.75 mg/kg, 1.5 mg/kg, and 3 mg/kg body weight. To achieve these aims, we investigated the effects of adrenaline on catalase (CAT), Cu, Zn-superoxide dismutase (SOD), malondialdehyde (MDA), nitrite (NO2-), carbonyl groups (PCC), and nitrotyrosine (3-NT). Total activity of lactate dehydrogenase (LDH), its relative distribution (LDH1-LDH5) activity, level of acute phase proteins (APPs), and genotoxic effect were also evaluated. The obtained results revealed that all doses of adrenaline induced a significant rise in CAT activity, MDA level, PCC, NO2-, and 3-NT and a significant decrease in SOD activity compared to control. Adrenaline exerted an increase in total activity of LDH, LDH1, and LDH2 isoenzymes. Further study showed that adrenaline significantly decreased serum albumin level and albumin-globulin ratio, while the level of APPs (alpha(1) -acid glycoprotein and haptoglobulin) is increased. The micronucleus test revealed a genotoxic effect of adrenaline at higher concentrations (1.5 mg/kg and 3 mg/kg body weight) compared to untreated rats. It can be concluded that adrenaline exerts oxidative and nitrative stress in rats, increased damage to lipids and proteins, and damage of cardiomyocytes and cytogenetic damage. Obtained results may contribute to better understanding of the toxicity of adrenaline with aims to preventing its harmful effects.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline
DO  - 10.1155/2018/1805354
ER  - 

@article{
author = "Radaković, Milena and Borozan, Sunčica and Đelić, Ninoslav and Ivanović, Sasa and Čupić-Miladinović, Dejana and Ristanić, Marko and Potparević, Biljana and Stanimirović, Zoran",
year = "2018",
abstract = "This study is aimed at analysing biochemical and genetic endpoints of toxic effects after administration of adrenaline. For this purpose, the study was carried out on Wistar rats and three doses of adrenaline were used: 0.75 mg/kg, 1.5 mg/kg, and 3 mg/kg body weight. To achieve these aims, we investigated the effects of adrenaline on catalase (CAT), Cu, Zn-superoxide dismutase (SOD), malondialdehyde (MDA), nitrite (NO2-), carbonyl groups (PCC), and nitrotyrosine (3-NT). Total activity of lactate dehydrogenase (LDH), its relative distribution (LDH1-LDH5) activity, level of acute phase proteins (APPs), and genotoxic effect were also evaluated. The obtained results revealed that all doses of adrenaline induced a significant rise in CAT activity, MDA level, PCC, NO2-, and 3-NT and a significant decrease in SOD activity compared to control. Adrenaline exerted an increase in total activity of LDH, LDH1, and LDH2 isoenzymes. Further study showed that adrenaline significantly decreased serum albumin level and albumin-globulin ratio, while the level of APPs (alpha(1) -acid glycoprotein and haptoglobulin) is increased. The micronucleus test revealed a genotoxic effect of adrenaline at higher concentrations (1.5 mg/kg and 3 mg/kg body weight) compared to untreated rats. It can be concluded that adrenaline exerts oxidative and nitrative stress in rats, increased damage to lipids and proteins, and damage of cardiomyocytes and cytogenetic damage. Obtained results may contribute to better understanding of the toxicity of adrenaline with aims to preventing its harmful effects.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline",
doi = "10.1155/2018/1805354"
}

Radaković, M., Borozan, S., Đelić, N., Ivanović, S., Čupić-Miladinović, D., Ristanić, M., Potparević, B.,& Stanimirović, Z.. (2018). Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London..
https://doi.org/10.1155/2018/1805354

Radaković M, Borozan S, Đelić N, Ivanović S, Čupić-Miladinović D, Ristanić M, Potparević B, Stanimirović Z. Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline. in Oxidative Medicine and Cellular Longevity. 2018;.
doi:10.1155/2018/1805354 .

Radaković, Milena, Borozan, Sunčica, Đelić, Ninoslav, Ivanović, Sasa, Čupić-Miladinović, Dejana, Ristanić, Marko, Potparević, Biljana, Stanimirović, Zoran, "Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline" in Oxidative Medicine and Cellular Longevity (2018),
https://doi.org/10.1155/2018/1805354 . .

TY  - JOUR
AU  - Bošnjak-Neumueller, Jasna
AU  - Radaković, Milena
AU  - Đelić, Ninoslav
AU  - Vuković-Gacić, Branka
AU  - Dajić-Stevanović, Zora
AU  - Kolarević, Stoimir
AU  - Misić, Danijela
AU  - Stanković, Milan
AU  - Knezević-Vukcević, Jelena
AU  - Potparević, Biljana
AU  - Stanimirović, Zoran
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2994
AB  - The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of agemg, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T-3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2-picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73 +/- 1.80 mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59 +/- 0.95 g/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0 mg/ml N. rtanjensis methanol extracts mixture with 100 mu M of T-3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS/MS).
PB  - Univ Karachi, Karachi
T2  - Pakistan journal of pharmaceutical sciences
T1  - Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes
VL  - 30
IS  - 2
SP  - 625
EP  - 634
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4504
ER  - 

@article{
author = "Bošnjak-Neumueller, Jasna and Radaković, Milena and Đelić, Ninoslav and Vuković-Gacić, Branka and Dajić-Stevanović, Zora and Kolarević, Stoimir and Misić, Danijela and Stanković, Milan and Knezević-Vukcević, Jelena and Potparević, Biljana and Stanimirović, Zoran",
year = "2017",
abstract = "The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of agemg, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T-3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2-picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73 +/- 1.80 mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59 +/- 0.95 g/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0 mg/ml N. rtanjensis methanol extracts mixture with 100 mu M of T-3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS/MS).",
publisher = "Univ Karachi, Karachi",
journal = "Pakistan journal of pharmaceutical sciences",
title = "Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes",
volume = "30",
number = "2",
pages = "625-634",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4504"
}

Bošnjak-Neumueller, J., Radaković, M., Đelić, N., Vuković-Gacić, B., Dajić-Stevanović, Z., Kolarević, S., Misić, D., Stanković, M., Knezević-Vukcević, J., Potparević, B.,& Stanimirović, Z.. (2017). Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes. in Pakistan journal of pharmaceutical sciences
Univ Karachi, Karachi., 30(2), 625-634.
https://hdl.handle.net/21.15107/rcub_agrospace_4504

Bošnjak-Neumueller J, Radaković M, Đelić N, Vuković-Gacić B, Dajić-Stevanović Z, Kolarević S, Misić D, Stanković M, Knezević-Vukcević J, Potparević B, Stanimirović Z. Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes. in Pakistan journal of pharmaceutical sciences. 2017;30(2):625-634.
https://hdl.handle.net/21.15107/rcub_agrospace_4504 .

Bošnjak-Neumueller, Jasna, Radaković, Milena, Đelić, Ninoslav, Vuković-Gacić, Branka, Dajić-Stevanović, Zora, Kolarević, Stoimir, Misić, Danijela, Stanković, Milan, Knezević-Vukcević, Jelena, Potparević, Biljana, Stanimirović, Zoran, "Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes" in Pakistan journal of pharmaceutical sciences, 30, no. 2 (2017):625-634,
https://hdl.handle.net/21.15107/rcub_agrospace_4504 .

TY  - JOUR
AU  - Nikolić, Tatjana V.
AU  - Purać, Jelena
AU  - Orčić, Snežana
AU  - Kojić, Danijela
AU  - Vujanović, Dragana
AU  - Stanimirović, Zoran
AU  - Gržetić, Ivan
AU  - Ilijević, Konstantin
AU  - Šikoparija, Branko
AU  - Blagojević, Duško P.
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2307
AB  - Understanding the cellular stress response in honey bees will significantly contribute to their conservation. The aim of this study was to analyze the response of the antioxidative enzymes superoxide dismutase and catalase in honey bees related to the presence of toxic metals in different habitats. Three locations were selected: (i) Tunovo on the mountain Golija, as control area, without industry and large human impact, (ii) Belgrade as urban area, and (iii) Zajaca, as mining and industrial zone. Our results showed that the concentrations of lead (Pb) in whole body of bees vary according to habitat, but there was very significant increase of Pb in bees from investigated industrial area. Bees from urban and industrial area had increased expression of both Sod1 and Cat genes, suggesting adaptation to increased oxidative stress. However, in spite increased gene expression, the enzyme activity of catalase was lower in bees from industrial area suggesting inhibitory effect of Pb on catalase.
PB  - Wiley-Blackwell, Hoboken
T2  - Archives of Insect Biochemistry and Physiology
T1  - Environmental Effects on Superoxide Dismutase and Catalase Activity and Expression in Honey Bee
VL  - 90
IS  - 4
SP  - 181
EP  - 194
DO  - 10.1002/arch.21253
ER  - 

@article{
author = "Nikolić, Tatjana V. and Purać, Jelena and Orčić, Snežana and Kojić, Danijela and Vujanović, Dragana and Stanimirović, Zoran and Gržetić, Ivan and Ilijević, Konstantin and Šikoparija, Branko and Blagojević, Duško P.",
year = "2015",
abstract = "Understanding the cellular stress response in honey bees will significantly contribute to their conservation. The aim of this study was to analyze the response of the antioxidative enzymes superoxide dismutase and catalase in honey bees related to the presence of toxic metals in different habitats. Three locations were selected: (i) Tunovo on the mountain Golija, as control area, without industry and large human impact, (ii) Belgrade as urban area, and (iii) Zajaca, as mining and industrial zone. Our results showed that the concentrations of lead (Pb) in whole body of bees vary according to habitat, but there was very significant increase of Pb in bees from investigated industrial area. Bees from urban and industrial area had increased expression of both Sod1 and Cat genes, suggesting adaptation to increased oxidative stress. However, in spite increased gene expression, the enzyme activity of catalase was lower in bees from industrial area suggesting inhibitory effect of Pb on catalase.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Archives of Insect Biochemistry and Physiology",
title = "Environmental Effects on Superoxide Dismutase and Catalase Activity and Expression in Honey Bee",
volume = "90",
number = "4",
pages = "181-194",
doi = "10.1002/arch.21253"
}

Nikolić, T. V., Purać, J., Orčić, S., Kojić, D., Vujanović, D., Stanimirović, Z., Gržetić, I., Ilijević, K., Šikoparija, B.,& Blagojević, D. P.. (2015). Environmental Effects on Superoxide Dismutase and Catalase Activity and Expression in Honey Bee. in Archives of Insect Biochemistry and Physiology
Wiley-Blackwell, Hoboken., 90(4), 181-194.
https://doi.org/10.1002/arch.21253

Nikolić TV, Purać J, Orčić S, Kojić D, Vujanović D, Stanimirović Z, Gržetić I, Ilijević K, Šikoparija B, Blagojević DP. Environmental Effects on Superoxide Dismutase and Catalase Activity and Expression in Honey Bee. in Archives of Insect Biochemistry and Physiology. 2015;90(4):181-194.
doi:10.1002/arch.21253 .

Nikolić, Tatjana V., Purać, Jelena, Orčić, Snežana, Kojić, Danijela, Vujanović, Dragana, Stanimirović, Zoran, Gržetić, Ivan, Ilijević, Konstantin, Šikoparija, Branko, Blagojević, Duško P., "Environmental Effects on Superoxide Dismutase and Catalase Activity and Expression in Honey Bee" in Archives of Insect Biochemistry and Physiology, 90, no. 4 (2015):181-194,
https://doi.org/10.1002/arch.21253 . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2411
AB  - Catechol groups can be involved in redox cycling accompanied by generation of reactive oxygen species (ROS) which may lead to oxidative damage of cellular macromolecules including DNA. The objective of this investigation was to evaluate possible genotoxic effects of a natural catecholamine adrenaline in cultured human lymphocytes using cytogenetic (sister chromatid exchange and micronuclei) and the single cell gel electrophoresis (Comet) assay. In cytogenetic tests, six experimental concentrations of adrenaline were used in a range from 0.01-500 mu M. There were no indications of genotoxic effects of adrenaline in sister chromatid exchange and micronucleus tests. However, at four highest concentrations of adrenaline (5 mu M, 50 mu M, 150 mu M and 300 mu M) we observed a decreased mitotic index and cell-cycle delay. In addition, in the Comet assay we used adrenaline in a range from 0.0005-500 mu M, at two treatment times: 15 min or 60 min. In contrast to cytogenetic analysis, there was a dose-dependent increase of DNA damage detected in the Comet assay. These effects were significantly reduced by concomitant treatment with quercetin or catalase. Therefore, the obtained results indicate that adrenaline may exhibit genotoxic effects in cultured human lymphocytes, most likely due to production of reactive oxygen species.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Toxicology Letters
T1  - Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro
VL  - 29
IS  - 1
SP  - 27
EP  - 33
DO  - 10.1016/j.tiv.2014.08.001
ER  - 

@article{
author = "Đelić, Ninoslav and Radaković, Milena and Potparević, Biljana and Živković, Lada and Bajić, Vladan and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2015",
abstract = "Catechol groups can be involved in redox cycling accompanied by generation of reactive oxygen species (ROS) which may lead to oxidative damage of cellular macromolecules including DNA. The objective of this investigation was to evaluate possible genotoxic effects of a natural catecholamine adrenaline in cultured human lymphocytes using cytogenetic (sister chromatid exchange and micronuclei) and the single cell gel electrophoresis (Comet) assay. In cytogenetic tests, six experimental concentrations of adrenaline were used in a range from 0.01-500 mu M. There were no indications of genotoxic effects of adrenaline in sister chromatid exchange and micronucleus tests. However, at four highest concentrations of adrenaline (5 mu M, 50 mu M, 150 mu M and 300 mu M) we observed a decreased mitotic index and cell-cycle delay. In addition, in the Comet assay we used adrenaline in a range from 0.0005-500 mu M, at two treatment times: 15 min or 60 min. In contrast to cytogenetic analysis, there was a dose-dependent increase of DNA damage detected in the Comet assay. These effects were significantly reduced by concomitant treatment with quercetin or catalase. Therefore, the obtained results indicate that adrenaline may exhibit genotoxic effects in cultured human lymphocytes, most likely due to production of reactive oxygen species.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Toxicology Letters",
title = "Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro",
volume = "29",
number = "1",
pages = "27-33",
doi = "10.1016/j.tiv.2014.08.001"
}

Đelić, N., Radaković, M., Potparević, B., Živković, L., Bajić, V., Stevanović, J.,& Stanimirović, Z.. (2015). Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro. in Toxicology Letters
Pergamon-Elsevier Science Ltd, Oxford., 29(1), 27-33.
https://doi.org/10.1016/j.tiv.2014.08.001

Đelić N, Radaković M, Potparević B, Živković L, Bajić V, Stevanović J, Stanimirović Z. Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro. in Toxicology Letters. 2015;29(1):27-33.
doi:10.1016/j.tiv.2014.08.001 .

Đelić, Ninoslav, Radaković, Milena, Potparević, Biljana, Živković, Lada, Bajić, Vladan, Stevanović, Jevrosima, Stanimirović, Zoran, "Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro" in Toxicology Letters, 29, no. 1 (2015):27-33,
https://doi.org/10.1016/j.tiv.2014.08.001 . .

TY  - JOUR
AU  - Radaković, Milena
AU  - Đelić, Ninoslav
AU  - Stanimirović, Zoran
AU  - Plećaš-Solarović, Bosiljka
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Bajić, Vladan
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1607
AB  - Ephedrine, a natural alkaloid from plants of the genus Ephedra, has a chemical structure similar to catecholamines. It is well established that catecholamines (adraneline, noradrenaline and dopamine) cause genotoxic and mutagenic effects. Therefore, the objectives of this investigation were to examine weather ephedrine can exhibit genotoxic effects on isolated human lymphocytes in the Comet assay. Dose-response of human lymphocytes was determined at the concentration range of ephedrine from 0.0005 μM to 500 μM. Three concentrations of ephedrine (1, 50 and 300 μM) which had acceptable cell viability (over 90%) were used for further experiments with inhibitors of DNA reparation (cytosine arabinoside and hydroxyurea). The obtained results showed that ephedrine did not induce DNA damage in isolated human lymphocytes. However, co-treatment of the negative control with DNA repair inhibitors caused a slight but significant increase of DNA damage, due to an endogenous DNA damage. Interestingly, cells treated with ephedrine and DNA repair inhibitors did not express increased DNA damage. On the basis of the obtained results it can be concluded that ephedrine did not exhibit genotoxic effects on isolated human lymphocytes. This result is in accordance with previous investigations showing negative genotoxicological results for ephedrine using bacterial gene mutation test-systems and in vitro cytogenetic analysis.
AB  - Efedrin, prirodni alkaloid iz biljaka roda Ephedra, ima sličnu hemijsku strukturu sa kateholaminima. Dobro je poznato da kateholamini (adrenalin, noradrenalin i dopamin) mogu da prouzrokuju genotoksične i mutagene efekte. Stoga su ciljevi ovog istraživanja bili da se ispita da li efedrin može da ispolji genotoksične efekte na izolovanim limfocitima čoveka u Komet testu. Odnos doza-efekat određ en je u rasponu koncentracija efedrina od 0.0005 μM do 500 μM. Tri koncentracije efedrina (1, 50 and 300 μM) koje su imale prihvatljiv nivo ćelijske vijabilnosti (preko 90%) upotrebljene su za dalje eksperimente sa inhibitorima reparacije DNK (citozin arabinozid i hidroksiurea). Dobijeni rezultati pokazuju da efedrin nije indukovao oštećenja DNK na izolovanim limfocitima čoveka. Međutim, istovremeni tretman sa inhibitorima reparacije DNK doveo je do malog ali statistički značajnog porasta oštećenja DNK kod negativne kontrole, usled endogenog oštećenja DNK. Interestantno je da ćelije tretirane sa efedrinom i inhibitorima reparacije DNK nisu ispoljile povećan nivo oštećenja DNK. Na osnovu dobijenih rezultata može se zaključiti da efedrin nije ispoljio genotoksične efekte na izolovanim limfocitima čoveka. Ovaj rezultat je u saglasnosti sa prethodnim istraživanjima u kojima je dokazano da efedrin ne dovodi do genotoksičnih efekata u bakterijskim testovima na genske mutacije i u in vitro citogenetičkim analizama.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Evaluation of the effects of ephedrine on human lymphocytes in the comet assay
T1  - Evaluacija dejstva efedrina na limfocite čoveka u komet testu
VL  - 61
IS  - 4
SP  - 363
EP  - 371
DO  - 10.2298/AVB1104363R
ER  - 

@article{
author = "Radaković, Milena and Đelić, Ninoslav and Stanimirović, Zoran and Plećaš-Solarović, Bosiljka and Spremo-Potparević, Biljana and Živković, Lada and Bajić, Vladan",
year = "2011",
abstract = "Ephedrine, a natural alkaloid from plants of the genus Ephedra, has a chemical structure similar to catecholamines. It is well established that catecholamines (adraneline, noradrenaline and dopamine) cause genotoxic and mutagenic effects. Therefore, the objectives of this investigation were to examine weather ephedrine can exhibit genotoxic effects on isolated human lymphocytes in the Comet assay. Dose-response of human lymphocytes was determined at the concentration range of ephedrine from 0.0005 μM to 500 μM. Three concentrations of ephedrine (1, 50 and 300 μM) which had acceptable cell viability (over 90%) were used for further experiments with inhibitors of DNA reparation (cytosine arabinoside and hydroxyurea). The obtained results showed that ephedrine did not induce DNA damage in isolated human lymphocytes. However, co-treatment of the negative control with DNA repair inhibitors caused a slight but significant increase of DNA damage, due to an endogenous DNA damage. Interestingly, cells treated with ephedrine and DNA repair inhibitors did not express increased DNA damage. On the basis of the obtained results it can be concluded that ephedrine did not exhibit genotoxic effects on isolated human lymphocytes. This result is in accordance with previous investigations showing negative genotoxicological results for ephedrine using bacterial gene mutation test-systems and in vitro cytogenetic analysis., Efedrin, prirodni alkaloid iz biljaka roda Ephedra, ima sličnu hemijsku strukturu sa kateholaminima. Dobro je poznato da kateholamini (adrenalin, noradrenalin i dopamin) mogu da prouzrokuju genotoksične i mutagene efekte. Stoga su ciljevi ovog istraživanja bili da se ispita da li efedrin može da ispolji genotoksične efekte na izolovanim limfocitima čoveka u Komet testu. Odnos doza-efekat određ en je u rasponu koncentracija efedrina od 0.0005 μM do 500 μM. Tri koncentracije efedrina (1, 50 and 300 μM) koje su imale prihvatljiv nivo ćelijske vijabilnosti (preko 90%) upotrebljene su za dalje eksperimente sa inhibitorima reparacije DNK (citozin arabinozid i hidroksiurea). Dobijeni rezultati pokazuju da efedrin nije indukovao oštećenja DNK na izolovanim limfocitima čoveka. Međutim, istovremeni tretman sa inhibitorima reparacije DNK doveo je do malog ali statistički značajnog porasta oštećenja DNK kod negativne kontrole, usled endogenog oštećenja DNK. Interestantno je da ćelije tretirane sa efedrinom i inhibitorima reparacije DNK nisu ispoljile povećan nivo oštećenja DNK. Na osnovu dobijenih rezultata može se zaključiti da efedrin nije ispoljio genotoksične efekte na izolovanim limfocitima čoveka. Ovaj rezultat je u saglasnosti sa prethodnim istraživanjima u kojima je dokazano da efedrin ne dovodi do genotoksičnih efekata u bakterijskim testovima na genske mutacije i u in vitro citogenetičkim analizama.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Evaluation of the effects of ephedrine on human lymphocytes in the comet assay, Evaluacija dejstva efedrina na limfocite čoveka u komet testu",
volume = "61",
number = "4",
pages = "363-371",
doi = "10.2298/AVB1104363R"
}

Radaković, M., Đelić, N., Stanimirović, Z., Plećaš-Solarović, B., Spremo-Potparević, B., Živković, L.,& Bajić, V.. (2011). Evaluation of the effects of ephedrine on human lymphocytes in the comet assay. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(4), 363-371.
https://doi.org/10.2298/AVB1104363R

Radaković M, Đelić N, Stanimirović Z, Plećaš-Solarović B, Spremo-Potparević B, Živković L, Bajić V. Evaluation of the effects of ephedrine on human lymphocytes in the comet assay. in Acta veterinaria. 2011;61(4):363-371.
doi:10.2298/AVB1104363R .

Radaković, Milena, Đelić, Ninoslav, Stanimirović, Zoran, Plećaš-Solarović, Bosiljka, Spremo-Potparević, Biljana, Živković, Lada, Bajić, Vladan, "Evaluation of the effects of ephedrine on human lymphocytes in the comet assay" in Acta veterinaria, 61, no. 4 (2011):363-371,
https://doi.org/10.2298/AVB1104363R . .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Milićević, Zorka
AU  - Živković, Lada
AU  - Potparević, Biljana
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1433
AB  - Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono and/or combinational therapies for cancer treatment.
AB  - Prevremena centromerna deoba (PCD) se može posmatrati kao manifestacija hromozomske nestabilnosti. U cilju procene efekta paklitaksela (Ptx) i cikloheksimida (Cy) na indukciju PCD-a, koristili smo mikronukleus test (CBMN) uz fluorescentnu in situ hibridizaciju (FISH), kao i test hromozomskih aberacija (CA) na humanim limfocitima periferne krvi. Rezultati su pokazali da Ptx sam, ili u kombinaciji sa Cy može da indukuje PCD. Ovi nalazi ukazuju da treba mnogo više pažnje obratiti na pojavu PCD-a kao parametra genotoksičnosti u prekliničkim ispitivanjima mono- i/ili politerapija za lečenje kancera.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro
T1  - Efekat samog paklitaksela, i u kombinaciji sa cikloheksimidom, na učestalost prevremene centromerne deobe in vitro
VL  - 62
IS  - 1
SP  - 63
EP  - 74
DO  - 10.2298/ABS1001063B
ER  - 

@article{
author = "Bajić, Vladan and Stanimirović, Zoran and Stevanović, Jevrosima and Milićević, Zorka and Živković, Lada and Potparević, Biljana",
year = "2010",
abstract = "Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono and/or combinational therapies for cancer treatment., Prevremena centromerna deoba (PCD) se može posmatrati kao manifestacija hromozomske nestabilnosti. U cilju procene efekta paklitaksela (Ptx) i cikloheksimida (Cy) na indukciju PCD-a, koristili smo mikronukleus test (CBMN) uz fluorescentnu in situ hibridizaciju (FISH), kao i test hromozomskih aberacija (CA) na humanim limfocitima periferne krvi. Rezultati su pokazali da Ptx sam, ili u kombinaciji sa Cy može da indukuje PCD. Ovi nalazi ukazuju da treba mnogo više pažnje obratiti na pojavu PCD-a kao parametra genotoksičnosti u prekliničkim ispitivanjima mono- i/ili politerapija za lečenje kancera.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro, Efekat samog paklitaksela, i u kombinaciji sa cikloheksimidom, na učestalost prevremene centromerne deobe in vitro",
volume = "62",
number = "1",
pages = "63-74",
doi = "10.2298/ABS1001063B"
}

Bajić, V., Stanimirović, Z., Stevanović, J., Milićević, Z., Živković, L.,& Potparević, B.. (2010). The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(1), 63-74.
https://doi.org/10.2298/ABS1001063B

Bajić V, Stanimirović Z, Stevanović J, Milićević Z, Živković L, Potparević B. The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro. in Archives of Biological Sciences. 2010;62(1):63-74.
doi:10.2298/ABS1001063B .

Bajić, Vladan, Stanimirović, Zoran, Stevanović, Jevrosima, Milićević, Zorka, Živković, Lada, Potparević, Biljana, "The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro" in Archives of Biological Sciences, 62, no. 1 (2010):63-74,
https://doi.org/10.2298/ABS1001063B . .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Milicević, Z.
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1233
AB  - Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3', 5' cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assail (CA) on human peripheral blood lymphocytes of healthy individuals and by hone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 pin) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p) injection of 8-Cl-cAMP in doses of 10, 80 and 15 0 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes
VL  - 14
IS  - 1
SP  - 71
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3670
ER  - 

@article{
author = "Bajić, Vladan and Stanimirović, Zoran and Stevanović, Jevrosima and Potparević, Biljana and Živković, Lada and Milicević, Z.",
year = "2009",
abstract = "Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3', 5' cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assail (CA) on human peripheral blood lymphocytes of healthy individuals and by hone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 pin) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p) injection of 8-Cl-cAMP in doses of 10, 80 and 15 0 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes",
volume = "14",
number = "1",
pages = "71-77",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3670"
}

Bajić, V., Stanimirović, Z., Stevanović, J., Potparević, B., Živković, L.,& Milicević, Z.. (2009). Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 14(1), 71-77.
https://hdl.handle.net/21.15107/rcub_vinar_3670

Bajić V, Stanimirović Z, Stevanović J, Potparević B, Živković L, Milicević Z. Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON. 2009;14(1):71-77.
https://hdl.handle.net/21.15107/rcub_vinar_3670 .

Bajić, Vladan, Stanimirović, Zoran, Stevanović, Jevrosima, Potparević, Biljana, Živković, Lada, Milicević, Z., "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes" in Journal of BUON, 14, no. 1 (2009):71-77,
https://hdl.handle.net/21.15107/rcub_vinar_3670 .