TY  - JOUR
AU  - Milić, Mirta
AU  - Ceppi, Marcello
AU  - Bruzzone, Marco
AU  - Azqueta, Amaya
AU  - Brunborg, Gunnar
AU  - Godschalk, Roger
AU  - Koppen, Gudrun
AU  - Langie, Sabine
AU  - Møller, Peter
AU  - Teixeira, João Paulo
AU  - Alija, Avdulla
AU  - Anderson, Diana
AU  - Andrade, Vanessa
AU  - Andreoli, Cristina
AU  - Asllani, Fisnik
AU  - Bangkoglu, Ezgi Eyluel
AU  - Barančoková, Magdalena
AU  - Basaran, Nursen
AU  - Boutet-Robinet, Elisa
AU  - Buschini, Annamaria
AU  - Cavallo, Delia
AU  - Costa Pereira, Cristiana
AU  - Costa, Carla
AU  - Costa, Solange
AU  - Da Silva, Juliana
AU  - Del Boˊ, Cristian
AU  - Dimitrijević Srećković, Vesna
AU  - Đelić, Ninoslav
AU  - Dobrzyńska, Malgorzata
AU  - Duračková, Zdenka
AU  - Dvořáková, Monika
AU  - Gajski, Goran
AU  - Galati, Serena
AU  - García Lima, Omar
AU  - Giovannelli, Lisa
AU  - Goroshinskaya, Irina A.
AU  - Grindel, Annemarie
AU  - Gutzkow, Kristine B.
AU  - Hernández, Alba
AU  - Hernández, Carlos
AU  - Holven, Kirsten B.
AU  - Ibero-Baraibar, Idoia
AU  - Ottestad, Inger
AU  - Kadioglu, Ela
AU  - Kažimirová, Alena
AU  - Kuznetsova, Elena
AU  - Ladeira, Carina
AU  - Laffon, Blanca
AU  - Lamonaca, Palma
AU  - Lebailly, Pierre
AU  - Louro, Henriqueta
AU  - Mandina Cardoso, Tania
AU  - Marcon, Francesca
AU  - Marcos, Ricard
AU  - Moretti, Massimo
AU  - Moretti, Silvia
AU  - Najafzadeh, Mojgan
AU  - Nemeth, Zsuzsanna
AU  - Neri, Monica
AU  - Novotna, Bozena
AU  - Orlow, Irene
AU  - Paduchova, Zuzana
AU  - Pastor, Susana
AU  - Perdry, Hervé
AU  - Spremo-Potparević, Biljana
AU  - Ramadhani, Dwi
AU  - Riso, Patrizia
AU  - Rohr, Paula
AU  - Rojas, Emilio
AU  - Rossner, Pavel
AU  - Safar, Anna
AU  - Sardas, Semra
AU  - Silva, Maria João
AU  - Sirota, Nikolay
AU  - Smolkova, Bozena
AU  - Staruchova, Marta
AU  - Stetina, Rudolf
AU  - Stopper, Helga
AU  - Surikova, Ekaterina I.
AU  - Ulven, Stine M.
AU  - Ursini, Cinzia Lucia
AU  - Valdiglesias, Vanessa
AU  - Valverde, Mahara
AU  - Vodicka, Pavel
AU  - Volkovova, Katarina
AU  - Wagner, Karl-Heinz
AU  - Živković, Lada
AU  - Dušinská, Maria
AU  - Collins, Andrew R.
AU  - Bonassi, Stefano
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3791
AB  - The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.
PB  - Elsevier B.V.
T2  - Mutation Research - Reviews in Mutation Research
T1  - The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders
VL  - 787
DO  - 10.1016/j.mrrev.2021.108371
ER  - 

@article{
author = "Milić, Mirta and Ceppi, Marcello and Bruzzone, Marco and Azqueta, Amaya and Brunborg, Gunnar and Godschalk, Roger and Koppen, Gudrun and Langie, Sabine and Møller, Peter and Teixeira, João Paulo and Alija, Avdulla and Anderson, Diana and Andrade, Vanessa and Andreoli, Cristina and Asllani, Fisnik and Bangkoglu, Ezgi Eyluel and Barančoková, Magdalena and Basaran, Nursen and Boutet-Robinet, Elisa and Buschini, Annamaria and Cavallo, Delia and Costa Pereira, Cristiana and Costa, Carla and Costa, Solange and Da Silva, Juliana and Del Boˊ, Cristian and Dimitrijević Srećković, Vesna and Đelić, Ninoslav and Dobrzyńska, Malgorzata and Duračková, Zdenka and Dvořáková, Monika and Gajski, Goran and Galati, Serena and García Lima, Omar and Giovannelli, Lisa and Goroshinskaya, Irina A. and Grindel, Annemarie and Gutzkow, Kristine B. and Hernández, Alba and Hernández, Carlos and Holven, Kirsten B. and Ibero-Baraibar, Idoia and Ottestad, Inger and Kadioglu, Ela and Kažimirová, Alena and Kuznetsova, Elena and Ladeira, Carina and Laffon, Blanca and Lamonaca, Palma and Lebailly, Pierre and Louro, Henriqueta and Mandina Cardoso, Tania and Marcon, Francesca and Marcos, Ricard and Moretti, Massimo and Moretti, Silvia and Najafzadeh, Mojgan and Nemeth, Zsuzsanna and Neri, Monica and Novotna, Bozena and Orlow, Irene and Paduchova, Zuzana and Pastor, Susana and Perdry, Hervé and Spremo-Potparević, Biljana and Ramadhani, Dwi and Riso, Patrizia and Rohr, Paula and Rojas, Emilio and Rossner, Pavel and Safar, Anna and Sardas, Semra and Silva, Maria João and Sirota, Nikolay and Smolkova, Bozena and Staruchova, Marta and Stetina, Rudolf and Stopper, Helga and Surikova, Ekaterina I. and Ulven, Stine M. and Ursini, Cinzia Lucia and Valdiglesias, Vanessa and Valverde, Mahara and Vodicka, Pavel and Volkovova, Katarina and Wagner, Karl-Heinz and Živković, Lada and Dušinská, Maria and Collins, Andrew R. and Bonassi, Stefano",
year = "2021",
abstract = "The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.",
publisher = "Elsevier B.V.",
journal = "Mutation Research - Reviews in Mutation Research",
title = "The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders",
volume = "787",
doi = "10.1016/j.mrrev.2021.108371"
}

Milić, M., Ceppi, M., Bruzzone, M., Azqueta, A., Brunborg, G., Godschalk, R., Koppen, G., Langie, S., Møller, P., Teixeira, J. P., Alija, A., Anderson, D., Andrade, V., Andreoli, C., Asllani, F., Bangkoglu, E. E., Barančoková, M., Basaran, N., Boutet-Robinet, E., Buschini, A., Cavallo, D., Costa Pereira, C., Costa, C., Costa, S., Da Silva, J., Del Boˊ, C., Dimitrijević Srećković, V., Đelić, N., Dobrzyńska, M., Duračková, Z., Dvořáková, M., Gajski, G., Galati, S., García Lima, O., Giovannelli, L., Goroshinskaya, I. A., Grindel, A., Gutzkow, K. B., Hernández, A., Hernández, C., Holven, K. B., Ibero-Baraibar, I., Ottestad, I., Kadioglu, E., Kažimirová, A., Kuznetsova, E., Ladeira, C., Laffon, B., Lamonaca, P., Lebailly, P., Louro, H., Mandina Cardoso, T., Marcon, F., Marcos, R., Moretti, M., Moretti, S., Najafzadeh, M., Nemeth, Z., Neri, M., Novotna, B., Orlow, I., Paduchova, Z., Pastor, S., Perdry, H., Spremo-Potparević, B., Ramadhani, D., Riso, P., Rohr, P., Rojas, E., Rossner, P., Safar, A., Sardas, S., Silva, M. J., Sirota, N., Smolkova, B., Staruchova, M., Stetina, R., Stopper, H., Surikova, E. I., Ulven, S. M., Ursini, C. L., Valdiglesias, V., Valverde, M., Vodicka, P., Volkovova, K., Wagner, K., Živković, L., Dušinská, M., Collins, A. R.,& Bonassi, S.. (2021). The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. in Mutation Research - Reviews in Mutation Research
Elsevier B.V.., 787.
https://doi.org/10.1016/j.mrrev.2021.108371

Milić M, Ceppi M, Bruzzone M, Azqueta A, Brunborg G, Godschalk R, Koppen G, Langie S, Møller P, Teixeira JP, Alija A, Anderson D, Andrade V, Andreoli C, Asllani F, Bangkoglu EE, Barančoková M, Basaran N, Boutet-Robinet E, Buschini A, Cavallo D, Costa Pereira C, Costa C, Costa S, Da Silva J, Del Boˊ C, Dimitrijević Srećković V, Đelić N, Dobrzyńska M, Duračková Z, Dvořáková M, Gajski G, Galati S, García Lima O, Giovannelli L, Goroshinskaya IA, Grindel A, Gutzkow KB, Hernández A, Hernández C, Holven KB, Ibero-Baraibar I, Ottestad I, Kadioglu E, Kažimirová A, Kuznetsova E, Ladeira C, Laffon B, Lamonaca P, Lebailly P, Louro H, Mandina Cardoso T, Marcon F, Marcos R, Moretti M, Moretti S, Najafzadeh M, Nemeth Z, Neri M, Novotna B, Orlow I, Paduchova Z, Pastor S, Perdry H, Spremo-Potparević B, Ramadhani D, Riso P, Rohr P, Rojas E, Rossner P, Safar A, Sardas S, Silva MJ, Sirota N, Smolkova B, Staruchova M, Stetina R, Stopper H, Surikova EI, Ulven SM, Ursini CL, Valdiglesias V, Valverde M, Vodicka P, Volkovova K, Wagner K, Živković L, Dušinská M, Collins AR, Bonassi S. The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. in Mutation Research - Reviews in Mutation Research. 2021;787.
doi:10.1016/j.mrrev.2021.108371 .

Milić, Mirta, Ceppi, Marcello, Bruzzone, Marco, Azqueta, Amaya, Brunborg, Gunnar, Godschalk, Roger, Koppen, Gudrun, Langie, Sabine, Møller, Peter, Teixeira, João Paulo, Alija, Avdulla, Anderson, Diana, Andrade, Vanessa, Andreoli, Cristina, Asllani, Fisnik, Bangkoglu, Ezgi Eyluel, Barančoková, Magdalena, Basaran, Nursen, Boutet-Robinet, Elisa, Buschini, Annamaria, Cavallo, Delia, Costa Pereira, Cristiana, Costa, Carla, Costa, Solange, Da Silva, Juliana, Del Boˊ, Cristian, Dimitrijević Srećković, Vesna, Đelić, Ninoslav, Dobrzyńska, Malgorzata, Duračková, Zdenka, Dvořáková, Monika, Gajski, Goran, Galati, Serena, García Lima, Omar, Giovannelli, Lisa, Goroshinskaya, Irina A., Grindel, Annemarie, Gutzkow, Kristine B., Hernández, Alba, Hernández, Carlos, Holven, Kirsten B., Ibero-Baraibar, Idoia, Ottestad, Inger, Kadioglu, Ela, Kažimirová, Alena, Kuznetsova, Elena, Ladeira, Carina, Laffon, Blanca, Lamonaca, Palma, Lebailly, Pierre, Louro, Henriqueta, Mandina Cardoso, Tania, Marcon, Francesca, Marcos, Ricard, Moretti, Massimo, Moretti, Silvia, Najafzadeh, Mojgan, Nemeth, Zsuzsanna, Neri, Monica, Novotna, Bozena, Orlow, Irene, Paduchova, Zuzana, Pastor, Susana, Perdry, Hervé, Spremo-Potparević, Biljana, Ramadhani, Dwi, Riso, Patrizia, Rohr, Paula, Rojas, Emilio, Rossner, Pavel, Safar, Anna, Sardas, Semra, Silva, Maria João, Sirota, Nikolay, Smolkova, Bozena, Staruchova, Marta, Stetina, Rudolf, Stopper, Helga, Surikova, Ekaterina I., Ulven, Stine M., Ursini, Cinzia Lucia, Valdiglesias, Vanessa, Valverde, Mahara, Vodicka, Pavel, Volkovova, Katarina, Wagner, Karl-Heinz, Živković, Lada, Dušinská, Maria, Collins, Andrew R., Bonassi, Stefano, "The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders" in Mutation Research - Reviews in Mutation Research, 787 (2021),
https://doi.org/10.1016/j.mrrev.2021.108371 . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3900
AB  - Objective. Inosine 5’-monophosphate dehydrogenase (IMPDH) activity in cancer cells is increased. Tiazofurin selectively inhibits the activity of IMPDH, and it has been granted for the treatment of different cancers and new viral diseases. Its widespread use was limited because exposure to tiazofurin under certain circumstances was found to have a higher frequency of severe non-hematologic toxicity. Therefore, the objective of this study was to examine genotoxic action and inducement of DNA damage of tiazofurin using the comet assay. Methods. The ability of tiazofurin to induce DNA damage was evaluated using single-cell gel electrophoresis (SCGE) technique/comet assay. Human whole blood cells were exposed to three final concentrations of tiazofurin (1 µM/mL, 2 µM/mL, and 5 µM/mL) for 30 min in vitro. Results. Our results indicate that tiazofurin produced a significant level of DNA damage on whole blood cells after 30 min of exposure vs. control. All tested concentrations were significantly comet-forming, in a concentration-dependent manner. Conclusion. Our investigation on the tiazofurin-treated cells and their relationship to the formation of DNA damage demonstrated that the genotoxic effect was induced after exposure to tiazofurin under described conditions.
AB  - Cilj. Aktivnost inozin 5'-monofosfat dehidrogenaze (IMPDH) povec'ana je u c'elijama karcinoma. Tiazofurin selektivno inhibira aktivnost IMPDH i odobren je za lečenje različitih karcinoma i novih virusnih bolesti. Njegova široko rasprostranjena upotreba bila je ograničena jer je utvrđeno da je izloženost tiazofurinu pod određenim okolnostima imala vec'u incidencu ozbiljne nehematološke toksičnosti. Stoga je cilj ove studije bio da se pomoc'u komet testa ispita genotoksično delovanje i izazivanje DNK oštec'enja tiazofurinom. Metode. Sposobnost tiazofurina da izazove DNK oštec'enje procenjena je primenom elektroforeze DNK pojedinačnih ćelija (SCGE) / komet testa. Ćelije pune krvi su bile izložene trima konačnim koncentracijama tiazofurina (1 µM/mL, 2 µM/mL, and 5 µM/mL) tokom 30 minuta in vitro. Rezultati. Naši rezultati ukazuju na to da je tiazofurin proizveo značajan nivo DNK oštec'enja na c'elijama pune krvi nakon 30 minuta izlaganja u odnosu na kontrolu. Sve ispitivane koncentracije su dovele do značajnog nastanka kometa, pri čemu je nivo oštećenja rastao s koncentracijom. Zaključak. Naše istraživanje c'elija tretiranih tiazofurinom i njihova reakcija na izazivanje DNK oštec'enja pokazalo je da je tiazofurin ispoljio genotoksični efekat pod opisanim uslovima.
PB  - Serbian Medical Society
T2  - Medicinski Casopis
T1  - Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay
T1  - Analiza DNK oštećenja izazvanog tiazofurinom u humanim ćelijama pune krvi primenom in vitro komet testa
VL  - 54
IS  - 3
SP  - 91
EP  - 95
DO  - 10.5937/mckg54-28798
ER  - 

@article{
author = "Topalović, Dijana and Živković, Lada and Đelić, Ninoslav and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Objective. Inosine 5’-monophosphate dehydrogenase (IMPDH) activity in cancer cells is increased. Tiazofurin selectively inhibits the activity of IMPDH, and it has been granted for the treatment of different cancers and new viral diseases. Its widespread use was limited because exposure to tiazofurin under certain circumstances was found to have a higher frequency of severe non-hematologic toxicity. Therefore, the objective of this study was to examine genotoxic action and inducement of DNA damage of tiazofurin using the comet assay. Methods. The ability of tiazofurin to induce DNA damage was evaluated using single-cell gel electrophoresis (SCGE) technique/comet assay. Human whole blood cells were exposed to three final concentrations of tiazofurin (1 µM/mL, 2 µM/mL, and 5 µM/mL) for 30 min in vitro. Results. Our results indicate that tiazofurin produced a significant level of DNA damage on whole blood cells after 30 min of exposure vs. control. All tested concentrations were significantly comet-forming, in a concentration-dependent manner. Conclusion. Our investigation on the tiazofurin-treated cells and their relationship to the formation of DNA damage demonstrated that the genotoxic effect was induced after exposure to tiazofurin under described conditions., Cilj. Aktivnost inozin 5'-monofosfat dehidrogenaze (IMPDH) povec'ana je u c'elijama karcinoma. Tiazofurin selektivno inhibira aktivnost IMPDH i odobren je za lečenje različitih karcinoma i novih virusnih bolesti. Njegova široko rasprostranjena upotreba bila je ograničena jer je utvrđeno da je izloženost tiazofurinu pod određenim okolnostima imala vec'u incidencu ozbiljne nehematološke toksičnosti. Stoga je cilj ove studije bio da se pomoc'u komet testa ispita genotoksično delovanje i izazivanje DNK oštec'enja tiazofurinom. Metode. Sposobnost tiazofurina da izazove DNK oštec'enje procenjena je primenom elektroforeze DNK pojedinačnih ćelija (SCGE) / komet testa. Ćelije pune krvi su bile izložene trima konačnim koncentracijama tiazofurina (1 µM/mL, 2 µM/mL, and 5 µM/mL) tokom 30 minuta in vitro. Rezultati. Naši rezultati ukazuju na to da je tiazofurin proizveo značajan nivo DNK oštec'enja na c'elijama pune krvi nakon 30 minuta izlaganja u odnosu na kontrolu. Sve ispitivane koncentracije su dovele do značajnog nastanka kometa, pri čemu je nivo oštećenja rastao s koncentracijom. Zaključak. Naše istraživanje c'elija tretiranih tiazofurinom i njihova reakcija na izazivanje DNK oštec'enja pokazalo je da je tiazofurin ispoljio genotoksični efekat pod opisanim uslovima.",
publisher = "Serbian Medical Society",
journal = "Medicinski Casopis",
title = "Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay, Analiza DNK oštećenja izazvanog tiazofurinom u humanim ćelijama pune krvi primenom in vitro komet testa",
volume = "54",
number = "3",
pages = "91-95",
doi = "10.5937/mckg54-28798"
}

Topalović, D., Živković, L., Đelić, N., Bajić, V.,& Spremo-Potparević, B.. (2020). Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay. in Medicinski Casopis
Serbian Medical Society., 54(3), 91-95.
https://doi.org/10.5937/mckg54-28798

Topalović D, Živković L, Đelić N, Bajić V, Spremo-Potparević B. Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay. in Medicinski Casopis. 2020;54(3):91-95.
doi:10.5937/mckg54-28798 .

Topalović, Dijana, Živković, Lada, Đelić, Ninoslav, Bajić, Vladan, Spremo-Potparević, Biljana, "Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay" in Medicinski Casopis, 54, no. 3 (2020):91-95,
https://doi.org/10.5937/mckg54-28798 . .

TY  - CONF
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Spremo-Potparević, Biljana
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Živković, Lada
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5150
AB  - Experimental evidence has shown the ability of adrenaline to cause changes in DNA, affirming that damaging effects occurs during the oxidation of the hormone. Longterm research in this area would surmise that the mechanisms of genotoxic effect of adrenaline are well understood, but the results of the studies are ambiguous. ...
PB  - National Health Institute Dr. Ricardo Jorge
PB  - Institute of Public Health of the University of Porto (ISPUP)
C3  - 4th International Congress on Occupational & Environmental Toxicology - ICOETox 2018, October 24-26, 2018, Porto, Portugal - Abstract book
T1  - Evaluation of adrenaline-induced DNA damage in vitro by comet assay
SP  - 135
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5150
ER  - 

@conference{
author = "Topalović, Dijana and Dekanski, Dragana and Spremo-Potparević, Biljana and Đelić, Ninoslav and Bajić, Vladan and Živković, Lada",
year = "2018",
abstract = "Experimental evidence has shown the ability of adrenaline to cause changes in DNA, affirming that damaging effects occurs during the oxidation of the hormone. Longterm research in this area would surmise that the mechanisms of genotoxic effect of adrenaline are well understood, but the results of the studies are ambiguous. ...",
publisher = "National Health Institute Dr. Ricardo Jorge, Institute of Public Health of the University of Porto (ISPUP)",
journal = "4th International Congress on Occupational & Environmental Toxicology - ICOETox 2018, October 24-26, 2018, Porto, Portugal - Abstract book",
title = "Evaluation of adrenaline-induced DNA damage in vitro by comet assay",
pages = "135-135",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5150"
}

Topalović, D., Dekanski, D., Spremo-Potparević, B., Đelić, N., Bajić, V.,& Živković, L.. (2018). Evaluation of adrenaline-induced DNA damage in vitro by comet assay. in 4th International Congress on Occupational & Environmental Toxicology - ICOETox 2018, October 24-26, 2018, Porto, Portugal - Abstract book
National Health Institute Dr. Ricardo Jorge., 135-135.
https://hdl.handle.net/21.15107/rcub_farfar_5150

Topalović D, Dekanski D, Spremo-Potparević B, Đelić N, Bajić V, Živković L. Evaluation of adrenaline-induced DNA damage in vitro by comet assay. in 4th International Congress on Occupational & Environmental Toxicology - ICOETox 2018, October 24-26, 2018, Porto, Portugal - Abstract book. 2018;:135-135.
https://hdl.handle.net/21.15107/rcub_farfar_5150 .

Topalović, Dijana, Dekanski, Dragana, Spremo-Potparević, Biljana, Đelić, Ninoslav, Bajić, Vladan, Živković, Lada, "Evaluation of adrenaline-induced DNA damage in vitro by comet assay" in 4th International Congress on Occupational & Environmental Toxicology - ICOETox 2018, October 24-26, 2018, Porto, Portugal - Abstract book (2018):135-135,
https://hdl.handle.net/21.15107/rcub_farfar_5150 .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Živković, Lada
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3131
AB  - Harmful effects of elevated levels of catecholamines are mediated by various mechanisms, including gene transcription and formation of oxidation products. The aim of this study was to see whether the molecular mechanisms underlying the damaging action of adrenaline on DNA are mediated by reactive oxygen species (ROS). To do that, we exposed human whole blood cells to 10 mu mol L-1 adrenaline or 50 mu mol L-1 H2O2 (used as positive control) that were separately pre-treated or post-treated with 500 mu mol L-1 of quercetin, a scavenger of free radicals. Quercetin significantly reduced DNA damage in both pre- and post-treatment protocols, which suggests that adrenaline mainly acts via the production of ROS. This mechanism is also supported by gradual lowering of adrenaline and H2O2-induced DNA damage 15, 30, 45, and 60 min after treatment. Our results clearly show that DNA repair mechanisms are rather effective against ROS-mediated DNA damage induced by adrenaline.
PB  - Inst Medical Research & Occupational Health, Zagreb
T2  - Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
T1  - Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay
VL  - 69
IS  - 4
SP  - 304
EP  - 308
DO  - 10.2478/aiht-2018-69-3154
ER  - 

@article{
author = "Topalović, Dijana and Dekanski, Dragana and Potparević, Biljana and Đelić, Ninoslav and Bajić, Vladan and Živković, Lada",
year = "2018",
abstract = "Harmful effects of elevated levels of catecholamines are mediated by various mechanisms, including gene transcription and formation of oxidation products. The aim of this study was to see whether the molecular mechanisms underlying the damaging action of adrenaline on DNA are mediated by reactive oxygen species (ROS). To do that, we exposed human whole blood cells to 10 mu mol L-1 adrenaline or 50 mu mol L-1 H2O2 (used as positive control) that were separately pre-treated or post-treated with 500 mu mol L-1 of quercetin, a scavenger of free radicals. Quercetin significantly reduced DNA damage in both pre- and post-treatment protocols, which suggests that adrenaline mainly acts via the production of ROS. This mechanism is also supported by gradual lowering of adrenaline and H2O2-induced DNA damage 15, 30, 45, and 60 min after treatment. Our results clearly show that DNA repair mechanisms are rather effective against ROS-mediated DNA damage induced by adrenaline.",
publisher = "Inst Medical Research & Occupational Health, Zagreb",
journal = "Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology",
title = "Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay",
volume = "69",
number = "4",
pages = "304-308",
doi = "10.2478/aiht-2018-69-3154"
}

Topalović, D., Dekanski, D., Potparević, B., Đelić, N., Bajić, V.,& Živković, L.. (2018). Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
Inst Medical Research & Occupational Health, Zagreb., 69(4), 304-308.
https://doi.org/10.2478/aiht-2018-69-3154

Topalović D, Dekanski D, Potparević B, Đelić N, Bajić V, Živković L. Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology. 2018;69(4):304-308.
doi:10.2478/aiht-2018-69-3154 .

Topalović, Dijana, Dekanski, Dragana, Potparević, Biljana, Đelić, Ninoslav, Bajić, Vladan, Živković, Lada, "Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay" in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology, 69, no. 4 (2018):304-308,
https://doi.org/10.2478/aiht-2018-69-3154 . .

TY  - JOUR
AU  - Radaković, Milena
AU  - Borozan, Sunčica
AU  - Đelić, Ninoslav
AU  - Ivanović, Sasa
AU  - Čupić-Miladinović, Dejana
AU  - Ristanić, Marko
AU  - Potparević, Biljana
AU  - Stanimirović, Zoran
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3064
AB  - This study is aimed at analysing biochemical and genetic endpoints of toxic effects after administration of adrenaline. For this purpose, the study was carried out on Wistar rats and three doses of adrenaline were used: 0.75 mg/kg, 1.5 mg/kg, and 3 mg/kg body weight. To achieve these aims, we investigated the effects of adrenaline on catalase (CAT), Cu, Zn-superoxide dismutase (SOD), malondialdehyde (MDA), nitrite (NO2-), carbonyl groups (PCC), and nitrotyrosine (3-NT). Total activity of lactate dehydrogenase (LDH), its relative distribution (LDH1-LDH5) activity, level of acute phase proteins (APPs), and genotoxic effect were also evaluated. The obtained results revealed that all doses of adrenaline induced a significant rise in CAT activity, MDA level, PCC, NO2-, and 3-NT and a significant decrease in SOD activity compared to control. Adrenaline exerted an increase in total activity of LDH, LDH1, and LDH2 isoenzymes. Further study showed that adrenaline significantly decreased serum albumin level and albumin-globulin ratio, while the level of APPs (alpha(1) -acid glycoprotein and haptoglobulin) is increased. The micronucleus test revealed a genotoxic effect of adrenaline at higher concentrations (1.5 mg/kg and 3 mg/kg body weight) compared to untreated rats. It can be concluded that adrenaline exerts oxidative and nitrative stress in rats, increased damage to lipids and proteins, and damage of cardiomyocytes and cytogenetic damage. Obtained results may contribute to better understanding of the toxicity of adrenaline with aims to preventing its harmful effects.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline
DO  - 10.1155/2018/1805354
ER  - 

@article{
author = "Radaković, Milena and Borozan, Sunčica and Đelić, Ninoslav and Ivanović, Sasa and Čupić-Miladinović, Dejana and Ristanić, Marko and Potparević, Biljana and Stanimirović, Zoran",
year = "2018",
abstract = "This study is aimed at analysing biochemical and genetic endpoints of toxic effects after administration of adrenaline. For this purpose, the study was carried out on Wistar rats and three doses of adrenaline were used: 0.75 mg/kg, 1.5 mg/kg, and 3 mg/kg body weight. To achieve these aims, we investigated the effects of adrenaline on catalase (CAT), Cu, Zn-superoxide dismutase (SOD), malondialdehyde (MDA), nitrite (NO2-), carbonyl groups (PCC), and nitrotyrosine (3-NT). Total activity of lactate dehydrogenase (LDH), its relative distribution (LDH1-LDH5) activity, level of acute phase proteins (APPs), and genotoxic effect were also evaluated. The obtained results revealed that all doses of adrenaline induced a significant rise in CAT activity, MDA level, PCC, NO2-, and 3-NT and a significant decrease in SOD activity compared to control. Adrenaline exerted an increase in total activity of LDH, LDH1, and LDH2 isoenzymes. Further study showed that adrenaline significantly decreased serum albumin level and albumin-globulin ratio, while the level of APPs (alpha(1) -acid glycoprotein and haptoglobulin) is increased. The micronucleus test revealed a genotoxic effect of adrenaline at higher concentrations (1.5 mg/kg and 3 mg/kg body weight) compared to untreated rats. It can be concluded that adrenaline exerts oxidative and nitrative stress in rats, increased damage to lipids and proteins, and damage of cardiomyocytes and cytogenetic damage. Obtained results may contribute to better understanding of the toxicity of adrenaline with aims to preventing its harmful effects.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline",
doi = "10.1155/2018/1805354"
}

Radaković, M., Borozan, S., Đelić, N., Ivanović, S., Čupić-Miladinović, D., Ristanić, M., Potparević, B.,& Stanimirović, Z.. (2018). Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London..
https://doi.org/10.1155/2018/1805354

Radaković M, Borozan S, Đelić N, Ivanović S, Čupić-Miladinović D, Ristanić M, Potparević B, Stanimirović Z. Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline. in Oxidative Medicine and Cellular Longevity. 2018;.
doi:10.1155/2018/1805354 .

Radaković, Milena, Borozan, Sunčica, Đelić, Ninoslav, Ivanović, Sasa, Čupić-Miladinović, Dejana, Ristanić, Marko, Potparević, Biljana, Stanimirović, Zoran, "Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline" in Oxidative Medicine and Cellular Longevity (2018),
https://doi.org/10.1155/2018/1805354 . .

TY  - JOUR
AU  - Bošnjak-Neumueller, Jasna
AU  - Radaković, Milena
AU  - Đelić, Ninoslav
AU  - Vuković-Gacić, Branka
AU  - Dajić-Stevanović, Zora
AU  - Kolarević, Stoimir
AU  - Misić, Danijela
AU  - Stanković, Milan
AU  - Knezević-Vukcević, Jelena
AU  - Potparević, Biljana
AU  - Stanimirović, Zoran
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2994
AB  - The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of agemg, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T-3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2-picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73 +/- 1.80 mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59 +/- 0.95 g/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0 mg/ml N. rtanjensis methanol extracts mixture with 100 mu M of T-3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS/MS).
PB  - Univ Karachi, Karachi
T2  - Pakistan journal of pharmaceutical sciences
T1  - Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes
VL  - 30
IS  - 2
SP  - 625
EP  - 634
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4504
ER  - 

@article{
author = "Bošnjak-Neumueller, Jasna and Radaković, Milena and Đelić, Ninoslav and Vuković-Gacić, Branka and Dajić-Stevanović, Zora and Kolarević, Stoimir and Misić, Danijela and Stanković, Milan and Knezević-Vukcević, Jelena and Potparević, Biljana and Stanimirović, Zoran",
year = "2017",
abstract = "The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of agemg, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T-3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2-picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73 +/- 1.80 mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59 +/- 0.95 g/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0 mg/ml N. rtanjensis methanol extracts mixture with 100 mu M of T-3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS/MS).",
publisher = "Univ Karachi, Karachi",
journal = "Pakistan journal of pharmaceutical sciences",
title = "Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes",
volume = "30",
number = "2",
pages = "625-634",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4504"
}

Bošnjak-Neumueller, J., Radaković, M., Đelić, N., Vuković-Gacić, B., Dajić-Stevanović, Z., Kolarević, S., Misić, D., Stanković, M., Knezević-Vukcević, J., Potparević, B.,& Stanimirović, Z.. (2017). Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes. in Pakistan journal of pharmaceutical sciences
Univ Karachi, Karachi., 30(2), 625-634.
https://hdl.handle.net/21.15107/rcub_agrospace_4504

Bošnjak-Neumueller J, Radaković M, Đelić N, Vuković-Gacić B, Dajić-Stevanović Z, Kolarević S, Misić D, Stanković M, Knezević-Vukcević J, Potparević B, Stanimirović Z. Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes. in Pakistan journal of pharmaceutical sciences. 2017;30(2):625-634.
https://hdl.handle.net/21.15107/rcub_agrospace_4504 .

Bošnjak-Neumueller, Jasna, Radaković, Milena, Đelić, Ninoslav, Vuković-Gacić, Branka, Dajić-Stevanović, Zora, Kolarević, Stoimir, Misić, Danijela, Stanković, Milan, Knezević-Vukcević, Jelena, Potparević, Biljana, Stanimirović, Zoran, "Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes" in Pakistan journal of pharmaceutical sciences, 30, no. 2 (2017):625-634,
https://hdl.handle.net/21.15107/rcub_agrospace_4504 .

TY  - JOUR
AU  - Vasiljević, Jovana
AU  - Živković, Lada
AU  - Čabarkapa, Andrea
AU  - Bajić, Vladan
AU  - Đelić, Ninoslav
AU  - Potparević, Biljana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2635
AB  - Context Cordyceps sinensis (C sinensis) is a well-known, traditional, Chinese medicinal mushroom, valued for its beneficial properties for human health. C sinensis has been reported to have immunomodulatory, anticancer, antiaging, antioxidant and anti-inflammatory activity. Despite potential medicinal benefits, no previously published reports are available about the genotoxicity or antigenotoxicity of C sinensis, as detected by comet assay. Objective The objective of the study was to evaluate both the genotoxic and antigenotoxic potential of an extract of C sinensis (CS extract) in human peripheral blood cells. Design The research team designed a pilot study. Setting The study was conducted at the Center for Biological Research, University of Belgrade, in Belgrade, Serbia. Participants Participants were 6 healthy individuals (2 males and 4 females), between the ages of 20 and 45 y, recruited on a voluntary basis, who provided heparinized, peripheral blood samples. Intervention Four concentrations of the CS extract125 mu g/mL, 250 mu g/mL, 500 mu g/mL, and 1000 mu g/mL-were used in the treatment of tested blood cells from the blood samples. Three independent procedures were performed: (1) a genotoxicity assessment, (2) an antigenotoxicity assessment for pretreatment of human cells with the CS extract prior to their exposure to hydrogen peroxide (H2O2) (ie, an evaluation of the benefits of the CS extract as a preventive agent); and (3) posttreatment of human cells with the CS extract after their exposure to H2O2 (ie, an evaluation of the benefits of the CS extract as an interventional agent). Outcome Measures Cells were graded by eye inspection into 5 classes, depending on the extent of DNA damage, representing: (1) class A-undamaged cells with no tail ( lt 5% damaged DNA); (2) class B-low-level damage (5%-20%); (3) class C-medium-level damage (20%-40%); (4) class D-high-level damage (40%-95%), and (5) class E-total destruction (>95%). Results The CS extract proved to be nongenotoxic because no induced DNA damage was detected at all tested concentrations. For the antigenotoxicity assessment of the pretreatment with the CS extract, only the 1000-mu g/mL concentration showed a significant decrease in the number of cells exhibiting H2O2-induced DNA damage. For the posttreatment, the CS extract exhibited antigenotoxic potential by attenuating H2O2-induced DNA damage at all concentrations tested. The evaluation of repair kinetics showed a decrease in DNA-damaged cells 15 min after the application of the CS extract, reaching a maximum potency after 45 min. Conclusions The results indicated that C sinensis can be used as a postapplicative agent that counteracts the effect of oxidative stress. The resulting reduction in DNA damage might be related to its scavenging properties and stimulation of DNA repair.
PB  - Innovision Communications, Aliso Viejo
T2  - Alternative Therapies in Health and Medicine
T1  - Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay
VL  - 22
SP  - 24
EP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_vinar_1311
ER  - 

@article{
author = "Vasiljević, Jovana and Živković, Lada and Čabarkapa, Andrea and Bajić, Vladan and Đelić, Ninoslav and Potparević, Biljana",
year = "2016",
abstract = "Context Cordyceps sinensis (C sinensis) is a well-known, traditional, Chinese medicinal mushroom, valued for its beneficial properties for human health. C sinensis has been reported to have immunomodulatory, anticancer, antiaging, antioxidant and anti-inflammatory activity. Despite potential medicinal benefits, no previously published reports are available about the genotoxicity or antigenotoxicity of C sinensis, as detected by comet assay. Objective The objective of the study was to evaluate both the genotoxic and antigenotoxic potential of an extract of C sinensis (CS extract) in human peripheral blood cells. Design The research team designed a pilot study. Setting The study was conducted at the Center for Biological Research, University of Belgrade, in Belgrade, Serbia. Participants Participants were 6 healthy individuals (2 males and 4 females), between the ages of 20 and 45 y, recruited on a voluntary basis, who provided heparinized, peripheral blood samples. Intervention Four concentrations of the CS extract125 mu g/mL, 250 mu g/mL, 500 mu g/mL, and 1000 mu g/mL-were used in the treatment of tested blood cells from the blood samples. Three independent procedures were performed: (1) a genotoxicity assessment, (2) an antigenotoxicity assessment for pretreatment of human cells with the CS extract prior to their exposure to hydrogen peroxide (H2O2) (ie, an evaluation of the benefits of the CS extract as a preventive agent); and (3) posttreatment of human cells with the CS extract after their exposure to H2O2 (ie, an evaluation of the benefits of the CS extract as an interventional agent). Outcome Measures Cells were graded by eye inspection into 5 classes, depending on the extent of DNA damage, representing: (1) class A-undamaged cells with no tail ( lt 5% damaged DNA); (2) class B-low-level damage (5%-20%); (3) class C-medium-level damage (20%-40%); (4) class D-high-level damage (40%-95%), and (5) class E-total destruction (>95%). Results The CS extract proved to be nongenotoxic because no induced DNA damage was detected at all tested concentrations. For the antigenotoxicity assessment of the pretreatment with the CS extract, only the 1000-mu g/mL concentration showed a significant decrease in the number of cells exhibiting H2O2-induced DNA damage. For the posttreatment, the CS extract exhibited antigenotoxic potential by attenuating H2O2-induced DNA damage at all concentrations tested. The evaluation of repair kinetics showed a decrease in DNA-damaged cells 15 min after the application of the CS extract, reaching a maximum potency after 45 min. Conclusions The results indicated that C sinensis can be used as a postapplicative agent that counteracts the effect of oxidative stress. The resulting reduction in DNA damage might be related to its scavenging properties and stimulation of DNA repair.",
publisher = "Innovision Communications, Aliso Viejo",
journal = "Alternative Therapies in Health and Medicine",
title = "Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay",
volume = "22",
pages = "24-31",
url = "https://hdl.handle.net/21.15107/rcub_vinar_1311"
}

Vasiljević, J., Živković, L., Čabarkapa, A., Bajić, V., Đelić, N.,& Potparević, B.. (2016). Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay. in Alternative Therapies in Health and Medicine
Innovision Communications, Aliso Viejo., 22, 24-31.
https://hdl.handle.net/21.15107/rcub_vinar_1311

Vasiljević J, Živković L, Čabarkapa A, Bajić V, Đelić N, Potparević B. Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay. in Alternative Therapies in Health and Medicine. 2016;22:24-31.
https://hdl.handle.net/21.15107/rcub_vinar_1311 .

Vasiljević, Jovana, Živković, Lada, Čabarkapa, Andrea, Bajić, Vladan, Đelić, Ninoslav, Potparević, Biljana, "Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay" in Alternative Therapies in Health and Medicine, 22 (2016):24-31,
https://hdl.handle.net/21.15107/rcub_vinar_1311 .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Pirković, Andrea
AU  - Jović, Slavoljub
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2484
AB  - Hormones are cellular products involved in the regulation of a large number of processes in living systems, and which by their actions affect the growth, function and metabo­lism of cells. Considering that hormones are compounds normally present in the organism, it is important to determine if they can, under certain circumstances, lead to genetic changes in the hereditary material. Numerous experimental studies in vitro and in vivo in different systems, from bacteria to mammals, dealt with the mutagenic and genotoxic effects of hormones. This work presents an overview of the research on genotoxic effects of non­steroidal hormones, although possible changes of genetic material under their influence have not still been known enough, and moreover, investigations on their genotoxic influ­ence have given conflicting results. The study results show that mechanisms of genotoxic effect of nonsteroidal hormones are manifested through the increase of oxidative stress by arising reactive oxygen species. A common mechanism of ROS occurence in thyroid hormones and catecholamines is through metabolic oxidation of their phenolic groups. Mani­festation of insulin genotoxic effect is based on production of ROS by activation of NADPH isophorms, while testing oxytocin showed absence of genotoxic effect. Considering that the investigations on genotoxicity of nonsteroidal hormones demonstrated both positive and negative results, the explanation of this discordance involve limitations of test systems themselves, different cell types or biological species used in the experiments, different lev­el of reactivity in vitro and in vivo, as well as possible variations in a tissue-specific expres­sion. Integrated, the provided data contribute to better understanding of genotoxic effect of nonsteroidal hormones and point out to the role and mode of action of these hormones in the process of occurring of effects caused by oxidative stress.
AB  - Hormoni su ćelijski proizvodi uključeni u regulaciju velikog broja procesa u živim sistemima koji svojim dejstvom utiču na rast, funkciju ili metabolizam ćelija. Obzirom da su hormoni jedinjenja koja su uobičajeno prisutna u organizmu, značajno je utvrditi da li oni mogu pod izvesnim okolnostima dovesti do genetičkih promena na naslednom materijalu. Eksperimentalna ispitivanja u in vitro i in vivo uslovima u različitim sistemima, od bakterija do sisara, bavila su se istraživanjem mutagenih i genotoksičnih efekata hormona. U ovom radu je dat pregled istraživanja genotoksičnih efekata nesteroidnih hormona, pošto još uvek nisu dovoljno poznate moguće promene naslednog materijala pod njihovim uticajem, a i ispitivanja njihovog genotoksičnog dejstva su dala oprečne rezultate. Rezultati studija pokazuju da se mehanizmi genotoksičnog dejstva nesteroidnih hormona ispoljavaju kroz povećanje oksidativnog stresa nastankom reaktivnih vrsta kiseonika (reactive oxygen species - ROS). Uobičajeni mehanizam nastanka ROS kod tireoidnih hormona i kateholamina je putem metaboličke oksidacije njihovih fenolnih grupa. Ispoljavanje genotoksičnog efekta insulina se zasniva na produkciji ROS putem aktivacije NADPH izoformi, dok je ispitivanje oksitocina pokazalo odsustvo genotoksičnog efekta. Uzimajući u obzir da su ispitivanja genotoksičnosti nesteroidnih hormona pokazala i pozitivne i negativne rezultate, objašnjenja ovog neslaganja obuhvataju ograničenja samih test sistema, različite tipove ćelije ili bioloških vrsta upotrebljenih u eksperimentima, različiti nivo reaktivnosti u in vitro i in vivo uslovima, kao i moguće razlike u tkivno specifičnoj ekspresiji. Objedinjeni, navedeni podaci doprinose boljem razumevanju genotoksičnih efekata nesteroidnih hormona i ukazuju na ulogu i načine delovanja ovih hormona u procesu nastanka efekata izazvanih oksidativnim stresom.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Genotoxic potential of nonsteroidal hormones
T1  - Genotoksični potencijal nesteroidnih hormona
VL  - 69
IS  - 3-4
SP  - 245
EP  - 257
DO  - 10.2298/VETGL1504245T
ER  - 

@article{
author = "Topalović, Dijana and Živković, Lada and Đelić, Ninoslav and Bajić, Vladan and Pirković, Andrea and Jović, Slavoljub and Spremo-Potparević, Biljana",
year = "2015",
abstract = "Hormones are cellular products involved in the regulation of a large number of processes in living systems, and which by their actions affect the growth, function and metabo­lism of cells. Considering that hormones are compounds normally present in the organism, it is important to determine if they can, under certain circumstances, lead to genetic changes in the hereditary material. Numerous experimental studies in vitro and in vivo in different systems, from bacteria to mammals, dealt with the mutagenic and genotoxic effects of hormones. This work presents an overview of the research on genotoxic effects of non­steroidal hormones, although possible changes of genetic material under their influence have not still been known enough, and moreover, investigations on their genotoxic influ­ence have given conflicting results. The study results show that mechanisms of genotoxic effect of nonsteroidal hormones are manifested through the increase of oxidative stress by arising reactive oxygen species. A common mechanism of ROS occurence in thyroid hormones and catecholamines is through metabolic oxidation of their phenolic groups. Mani­festation of insulin genotoxic effect is based on production of ROS by activation of NADPH isophorms, while testing oxytocin showed absence of genotoxic effect. Considering that the investigations on genotoxicity of nonsteroidal hormones demonstrated both positive and negative results, the explanation of this discordance involve limitations of test systems themselves, different cell types or biological species used in the experiments, different lev­el of reactivity in vitro and in vivo, as well as possible variations in a tissue-specific expres­sion. Integrated, the provided data contribute to better understanding of genotoxic effect of nonsteroidal hormones and point out to the role and mode of action of these hormones in the process of occurring of effects caused by oxidative stress., Hormoni su ćelijski proizvodi uključeni u regulaciju velikog broja procesa u živim sistemima koji svojim dejstvom utiču na rast, funkciju ili metabolizam ćelija. Obzirom da su hormoni jedinjenja koja su uobičajeno prisutna u organizmu, značajno je utvrditi da li oni mogu pod izvesnim okolnostima dovesti do genetičkih promena na naslednom materijalu. Eksperimentalna ispitivanja u in vitro i in vivo uslovima u različitim sistemima, od bakterija do sisara, bavila su se istraživanjem mutagenih i genotoksičnih efekata hormona. U ovom radu je dat pregled istraživanja genotoksičnih efekata nesteroidnih hormona, pošto još uvek nisu dovoljno poznate moguće promene naslednog materijala pod njihovim uticajem, a i ispitivanja njihovog genotoksičnog dejstva su dala oprečne rezultate. Rezultati studija pokazuju da se mehanizmi genotoksičnog dejstva nesteroidnih hormona ispoljavaju kroz povećanje oksidativnog stresa nastankom reaktivnih vrsta kiseonika (reactive oxygen species - ROS). Uobičajeni mehanizam nastanka ROS kod tireoidnih hormona i kateholamina je putem metaboličke oksidacije njihovih fenolnih grupa. Ispoljavanje genotoksičnog efekta insulina se zasniva na produkciji ROS putem aktivacije NADPH izoformi, dok je ispitivanje oksitocina pokazalo odsustvo genotoksičnog efekta. Uzimajući u obzir da su ispitivanja genotoksičnosti nesteroidnih hormona pokazala i pozitivne i negativne rezultate, objašnjenja ovog neslaganja obuhvataju ograničenja samih test sistema, različite tipove ćelije ili bioloških vrsta upotrebljenih u eksperimentima, različiti nivo reaktivnosti u in vitro i in vivo uslovima, kao i moguće razlike u tkivno specifičnoj ekspresiji. Objedinjeni, navedeni podaci doprinose boljem razumevanju genotoksičnih efekata nesteroidnih hormona i ukazuju na ulogu i načine delovanja ovih hormona u procesu nastanka efekata izazvanih oksidativnim stresom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Genotoxic potential of nonsteroidal hormones, Genotoksični potencijal nesteroidnih hormona",
volume = "69",
number = "3-4",
pages = "245-257",
doi = "10.2298/VETGL1504245T"
}

Topalović, D., Živković, L., Đelić, N., Bajić, V., Pirković, A., Jović, S.,& Spremo-Potparević, B.. (2015). Genotoxic potential of nonsteroidal hormones. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(3-4), 245-257.
https://doi.org/10.2298/VETGL1504245T

Topalović D, Živković L, Đelić N, Bajić V, Pirković A, Jović S, Spremo-Potparević B. Genotoxic potential of nonsteroidal hormones. in Veterinarski glasnik. 2015;69(3-4):245-257.
doi:10.2298/VETGL1504245T .

Topalović, Dijana, Živković, Lada, Đelić, Ninoslav, Bajić, Vladan, Pirković, Andrea, Jović, Slavoljub, Spremo-Potparević, Biljana, "Genotoxic potential of nonsteroidal hormones" in Veterinarski glasnik, 69, no. 3-4 (2015):245-257,
https://doi.org/10.2298/VETGL1504245T . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Pirković, Andrea
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2384
AB  - The thyroid hormones change the rate of basal metabolism, modulating the consumption of oxygen and causing production of reactive oxygen species, which leads to the development of oxidative stress and DNA strand breaks. Olive (Olea europaea L.) leaf contains many potentially bioactive compounds, making it one of the most potent natural antioxidants. The objective of this study was to evaluate the genotoxicity of L-thyroxine and to investigate antioxidative and antigenotoxic potential of the standardized oleuropein-rich dry olive leaf extract (DOLE) against hydrogen peroxide and L-thyroxine-induced DNA damage in human peripheral blood leukocytes by using the comet assay. Various concentrations of the extract were tested with both DNA damage inducers, under two different experimental conditions, pretreatment and posttreatment. Results indicate that L-thyroxine exhibited genotoxic effect and that DOLE displayed protective effect against thyroxine-induced genotoxicity. The number of cells with DNA damage, was significantly reduced, in both pretreated and posttreated samples (P  lt  0.05). Comparing the beneficial effect of all tested concentrations of DOLE, in both experimental protocols, it appears that extract was more effective in reducing DNA damage in the pretreatment, exhibiting protective role against L-thyroxine effect. This feature of DOLE can be explained by its capacity to act as potent free radical scavenger.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro
DO  - 10.1155/2015/762192
ER  - 

@article{
author = "Topalović, Dijana and Živković, Lada and Pirković, Andrea and Đelić, Ninoslav and Bajić, Vladan and Dekanski, Dragana and Potparević, Biljana",
year = "2015",
abstract = "The thyroid hormones change the rate of basal metabolism, modulating the consumption of oxygen and causing production of reactive oxygen species, which leads to the development of oxidative stress and DNA strand breaks. Olive (Olea europaea L.) leaf contains many potentially bioactive compounds, making it one of the most potent natural antioxidants. The objective of this study was to evaluate the genotoxicity of L-thyroxine and to investigate antioxidative and antigenotoxic potential of the standardized oleuropein-rich dry olive leaf extract (DOLE) against hydrogen peroxide and L-thyroxine-induced DNA damage in human peripheral blood leukocytes by using the comet assay. Various concentrations of the extract were tested with both DNA damage inducers, under two different experimental conditions, pretreatment and posttreatment. Results indicate that L-thyroxine exhibited genotoxic effect and that DOLE displayed protective effect against thyroxine-induced genotoxicity. The number of cells with DNA damage, was significantly reduced, in both pretreated and posttreated samples (P  lt  0.05). Comparing the beneficial effect of all tested concentrations of DOLE, in both experimental protocols, it appears that extract was more effective in reducing DNA damage in the pretreatment, exhibiting protective role against L-thyroxine effect. This feature of DOLE can be explained by its capacity to act as potent free radical scavenger.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro",
doi = "10.1155/2015/762192"
}

Topalović, D., Živković, L., Pirković, A., Đelić, N., Bajić, V., Dekanski, D.,& Potparević, B.. (2015). Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London..
https://doi.org/10.1155/2015/762192

Topalović D, Živković L, Pirković A, Đelić N, Bajić V, Dekanski D, Potparević B. Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro. in Oxidative Medicine and Cellular Longevity. 2015;.
doi:10.1155/2015/762192 .

Topalović, Dijana, Živković, Lada, Pirković, Andrea, Đelić, Ninoslav, Bajić, Vladan, Dekanski, Dragana, Potparević, Biljana, "Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro" in Oxidative Medicine and Cellular Longevity (2015),
https://doi.org/10.1155/2015/762192 . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2411
AB  - Catechol groups can be involved in redox cycling accompanied by generation of reactive oxygen species (ROS) which may lead to oxidative damage of cellular macromolecules including DNA. The objective of this investigation was to evaluate possible genotoxic effects of a natural catecholamine adrenaline in cultured human lymphocytes using cytogenetic (sister chromatid exchange and micronuclei) and the single cell gel electrophoresis (Comet) assay. In cytogenetic tests, six experimental concentrations of adrenaline were used in a range from 0.01-500 mu M. There were no indications of genotoxic effects of adrenaline in sister chromatid exchange and micronucleus tests. However, at four highest concentrations of adrenaline (5 mu M, 50 mu M, 150 mu M and 300 mu M) we observed a decreased mitotic index and cell-cycle delay. In addition, in the Comet assay we used adrenaline in a range from 0.0005-500 mu M, at two treatment times: 15 min or 60 min. In contrast to cytogenetic analysis, there was a dose-dependent increase of DNA damage detected in the Comet assay. These effects were significantly reduced by concomitant treatment with quercetin or catalase. Therefore, the obtained results indicate that adrenaline may exhibit genotoxic effects in cultured human lymphocytes, most likely due to production of reactive oxygen species.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Toxicology Letters
T1  - Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro
VL  - 29
IS  - 1
SP  - 27
EP  - 33
DO  - 10.1016/j.tiv.2014.08.001
ER  - 

@article{
author = "Đelić, Ninoslav and Radaković, Milena and Potparević, Biljana and Živković, Lada and Bajić, Vladan and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2015",
abstract = "Catechol groups can be involved in redox cycling accompanied by generation of reactive oxygen species (ROS) which may lead to oxidative damage of cellular macromolecules including DNA. The objective of this investigation was to evaluate possible genotoxic effects of a natural catecholamine adrenaline in cultured human lymphocytes using cytogenetic (sister chromatid exchange and micronuclei) and the single cell gel electrophoresis (Comet) assay. In cytogenetic tests, six experimental concentrations of adrenaline were used in a range from 0.01-500 mu M. There were no indications of genotoxic effects of adrenaline in sister chromatid exchange and micronucleus tests. However, at four highest concentrations of adrenaline (5 mu M, 50 mu M, 150 mu M and 300 mu M) we observed a decreased mitotic index and cell-cycle delay. In addition, in the Comet assay we used adrenaline in a range from 0.0005-500 mu M, at two treatment times: 15 min or 60 min. In contrast to cytogenetic analysis, there was a dose-dependent increase of DNA damage detected in the Comet assay. These effects were significantly reduced by concomitant treatment with quercetin or catalase. Therefore, the obtained results indicate that adrenaline may exhibit genotoxic effects in cultured human lymphocytes, most likely due to production of reactive oxygen species.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Toxicology Letters",
title = "Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro",
volume = "29",
number = "1",
pages = "27-33",
doi = "10.1016/j.tiv.2014.08.001"
}

Đelić, N., Radaković, M., Potparević, B., Živković, L., Bajić, V., Stevanović, J.,& Stanimirović, Z.. (2015). Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro. in Toxicology Letters
Pergamon-Elsevier Science Ltd, Oxford., 29(1), 27-33.
https://doi.org/10.1016/j.tiv.2014.08.001

Đelić N, Radaković M, Potparević B, Živković L, Bajić V, Stevanović J, Stanimirović Z. Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro. in Toxicology Letters. 2015;29(1):27-33.
doi:10.1016/j.tiv.2014.08.001 .

Đelić, Ninoslav, Radaković, Milena, Potparević, Biljana, Živković, Lada, Bajić, Vladan, Stevanović, Jevrosima, Stanimirović, Zoran, "Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro" in Toxicology Letters, 29, no. 1 (2015):27-33,
https://doi.org/10.1016/j.tiv.2014.08.001 . .

TY  - CONF
AU  - Žukovec-Topalović, Dijana
AU  - Čabarkapa, Andrea
AU  - Živković, Lada
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Dekanski, Dekanski
AU  - Spremo-Potparević, Biljana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5129
AB  - Both hormones thyroxin and adrenaline increase endogenous formation of reactive oxygen species (ROS), leading to oxidative stress and DNA damage. Dry olive leaf extract (DOLE) is plant product known to modulate effects of oxidants in human cells. Aim of our study was to evaluate ability of commercial DOLE to protect nuclear DNA from oxidative damage induced by different oxidants and to compare level of protective effect by using comet assay. Peripheral blood leukocytes were treated in vitro with DOLE under two protocols. First group of samples was exposed to oxidant and afterwards with three concentrations of DOLE (0,125, 0,5 and 1 mg/ml). Second group was pretreated with DOLE under same conditions, followed by the administration of oxidant. Effect of the extract with adrenaline treatment was most beneficial at smallest concentrations (0,125 mg/ml), while in treatment with thyroxin DOLE was most effective at 1 mg/ml in pretreatment and at 0.5 mg/ml in posttreatment. DOLEs protective effect was noticeable under both protocols compared to only oxidant exposed controls. Using the comet assay methodology, we were able to detect antioxidant and genoprotective properties of DOLE.
PB  - Serbian Genetic Society, Belgrade
C3  - V Congress of Serbian genetic Society, 28.09. - 02.10.2014 Kladovo, Serbia
T1  - DOLE protects leucocytes from hormone-induced DNA damage in vitro
SP  - 142
EP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5129
ER  - 

@conference{
author = "Žukovec-Topalović, Dijana and Čabarkapa, Andrea and Živković, Lada and Đelić, Ninoslav and Bajić, Vladan and Dekanski, Dekanski and Spremo-Potparević, Biljana",
year = "2014",
abstract = "Both hormones thyroxin and adrenaline increase endogenous formation of reactive oxygen species (ROS), leading to oxidative stress and DNA damage. Dry olive leaf extract (DOLE) is plant product known to modulate effects of oxidants in human cells. Aim of our study was to evaluate ability of commercial DOLE to protect nuclear DNA from oxidative damage induced by different oxidants and to compare level of protective effect by using comet assay. Peripheral blood leukocytes were treated in vitro with DOLE under two protocols. First group of samples was exposed to oxidant and afterwards with three concentrations of DOLE (0,125, 0,5 and 1 mg/ml). Second group was pretreated with DOLE under same conditions, followed by the administration of oxidant. Effect of the extract with adrenaline treatment was most beneficial at smallest concentrations (0,125 mg/ml), while in treatment with thyroxin DOLE was most effective at 1 mg/ml in pretreatment and at 0.5 mg/ml in posttreatment. DOLEs protective effect was noticeable under both protocols compared to only oxidant exposed controls. Using the comet assay methodology, we were able to detect antioxidant and genoprotective properties of DOLE.",
publisher = "Serbian Genetic Society, Belgrade",
journal = "V Congress of Serbian genetic Society, 28.09. - 02.10.2014 Kladovo, Serbia",
title = "DOLE protects leucocytes from hormone-induced DNA damage in vitro",
pages = "142-142",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5129"
}

Žukovec-Topalović, D., Čabarkapa, A., Živković, L., Đelić, N., Bajić, V., Dekanski, D.,& Spremo-Potparević, B.. (2014). DOLE protects leucocytes from hormone-induced DNA damage in vitro. in V Congress of Serbian genetic Society, 28.09. - 02.10.2014 Kladovo, Serbia
Serbian Genetic Society, Belgrade., 142-142.
https://hdl.handle.net/21.15107/rcub_farfar_5129

Žukovec-Topalović D, Čabarkapa A, Živković L, Đelić N, Bajić V, Dekanski D, Spremo-Potparević B. DOLE protects leucocytes from hormone-induced DNA damage in vitro. in V Congress of Serbian genetic Society, 28.09. - 02.10.2014 Kladovo, Serbia. 2014;:142-142.
https://hdl.handle.net/21.15107/rcub_farfar_5129 .

Žukovec-Topalović, Dijana, Čabarkapa, Andrea, Živković, Lada, Đelić, Ninoslav, Bajić, Vladan, Dekanski, Dekanski, Spremo-Potparević, Biljana, "DOLE protects leucocytes from hormone-induced DNA damage in vitro" in V Congress of Serbian genetic Society, 28.09. - 02.10.2014 Kladovo, Serbia (2014):142-142,
https://hdl.handle.net/21.15107/rcub_farfar_5129 .

TY  - JOUR
AU  - Pirković, Andrea
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2156
AB  - Excessive release of stress hormone adrenaline is accompanied by generation of reactive oxygen species which may cause disruption of DNA integrity leading to cancer and age-related disorders. Phenolic-rich plant product dry olive leaf extract (DOLE) is known to modulate effects of various oxidants in human cells. The aim was to evaluate the effect of commercial DOLE against adrenaline induced DNA damage in human leukocytes by using comet assay. Peripheral blood leukocytes from 6 healthy subjects were treated in vitro with three final concentrations of DOLE (0.125, 0.5, and 1 mg/mL) for 30 min at 37 degrees C under two different protocols, pretreatment and post-treatment. Protective effect of DOLE was assessed from its ability to attenuate formation of DNA lesions induced by adrenaline. Compared to cells exposed only to adrenaline, DOLE displayed significant reduction (P  lt  0.001) of DNA damage at all three concentrations and under both experimental protocols. Pearson correlation analysis revealed a significant positive association between DOLE concentration and leukocytes DNA damage (P  lt  0.05). Antigenotoxic effect of the extract was more pronounced at smaller concentrations. Post-treatment with 0.125 mg/mL DOLE was the most effective against adrenaline genotoxicity. Results indicate genoprotective and antioxidant properties in dry olive leaf extract, strongly supporting further explorations of its underlying mechanisms of action.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Toxicology Letters
T1  - Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes
VL  - 28
IS  - 3
SP  - 451
EP  - 456
DO  - 10.1016/j.tiv.2013.12.014
ER  - 

@article{
author = "Pirković, Andrea and Živković, Lada and Topalović, Dijana and Đelić, Ninoslav and Bajić, Vladan and Dekanski, Dragana and Potparević, Biljana",
year = "2014",
abstract = "Excessive release of stress hormone adrenaline is accompanied by generation of reactive oxygen species which may cause disruption of DNA integrity leading to cancer and age-related disorders. Phenolic-rich plant product dry olive leaf extract (DOLE) is known to modulate effects of various oxidants in human cells. The aim was to evaluate the effect of commercial DOLE against adrenaline induced DNA damage in human leukocytes by using comet assay. Peripheral blood leukocytes from 6 healthy subjects were treated in vitro with three final concentrations of DOLE (0.125, 0.5, and 1 mg/mL) for 30 min at 37 degrees C under two different protocols, pretreatment and post-treatment. Protective effect of DOLE was assessed from its ability to attenuate formation of DNA lesions induced by adrenaline. Compared to cells exposed only to adrenaline, DOLE displayed significant reduction (P  lt  0.001) of DNA damage at all three concentrations and under both experimental protocols. Pearson correlation analysis revealed a significant positive association between DOLE concentration and leukocytes DNA damage (P  lt  0.05). Antigenotoxic effect of the extract was more pronounced at smaller concentrations. Post-treatment with 0.125 mg/mL DOLE was the most effective against adrenaline genotoxicity. Results indicate genoprotective and antioxidant properties in dry olive leaf extract, strongly supporting further explorations of its underlying mechanisms of action.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Toxicology Letters",
title = "Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes",
volume = "28",
number = "3",
pages = "451-456",
doi = "10.1016/j.tiv.2013.12.014"
}

Pirković, A., Živković, L., Topalović, D., Đelić, N., Bajić, V., Dekanski, D.,& Potparević, B.. (2014). Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes. in Toxicology Letters
Pergamon-Elsevier Science Ltd, Oxford., 28(3), 451-456.
https://doi.org/10.1016/j.tiv.2013.12.014

Pirković A, Živković L, Topalović D, Đelić N, Bajić V, Dekanski D, Potparević B. Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes. in Toxicology Letters. 2014;28(3):451-456.
doi:10.1016/j.tiv.2013.12.014 .

Pirković, Andrea, Živković, Lada, Topalović, Dijana, Đelić, Ninoslav, Bajić, Vladan, Dekanski, Dragana, Potparević, Biljana, "Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes" in Toxicology Letters, 28, no. 3 (2014):451-456,
https://doi.org/10.1016/j.tiv.2013.12.014 . .

TY  - JOUR
AU  - Plećaš-Solarović, Bosiljka
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Živković, Lada
AU  - Potparević, Biljana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2088
AB  - Alzheimer's disease (AD) is the most frequent progressive neurodegenerative disorder in elderly associated with irreversible cognitive impairment and dementia. The vast majority of AD patients are sporadic (SAD) in which the disease develops after age of 65. Despite of century of research, we lack understanding of the SAD etiology and pathogenesis. Several hypotheses try to explain the main causes of brain degeneration in SAD, one of them assuming that genomic instability and the reentry of certain neurons into the incomplete cell cycle may be the pathogenic basis of the disease. Although the brain is the most affected organ in AD, numerous studies showed structural and functional alterations in peripheral tissues, suggesting that AD is a generalized systemic disorder. Diverse changes in peripheral cells from AD patients are described in literature including cell cycle aberration and chromosome instability, alterations in cell viability, proliferation and apoptosis, oxidative metabolism, amyloid precursor protein and amyloid beta protein metabolism, and other cellular processes. The aim of this paper was to summarize and review the results of our investigations and the growing literature data concerning the multiple chromosomal alterations in peripheral cells of AD patients and to consider their possible role in the disease pathogenesis as well as the importance of such investigations.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika, Belgrade
T1  - Cytogenetic alterations in peripheral cells of alzheimer's disease patients
VL  - 46
IS  - 1
SP  - 315
EP  - 330
DO  - 10.2298/GENSR1401315P
ER  - 

@article{
author = "Plećaš-Solarović, Bosiljka and Đelić, Ninoslav and Bajić, Vladan and Živković, Lada and Potparević, Biljana",
year = "2014",
abstract = "Alzheimer's disease (AD) is the most frequent progressive neurodegenerative disorder in elderly associated with irreversible cognitive impairment and dementia. The vast majority of AD patients are sporadic (SAD) in which the disease develops after age of 65. Despite of century of research, we lack understanding of the SAD etiology and pathogenesis. Several hypotheses try to explain the main causes of brain degeneration in SAD, one of them assuming that genomic instability and the reentry of certain neurons into the incomplete cell cycle may be the pathogenic basis of the disease. Although the brain is the most affected organ in AD, numerous studies showed structural and functional alterations in peripheral tissues, suggesting that AD is a generalized systemic disorder. Diverse changes in peripheral cells from AD patients are described in literature including cell cycle aberration and chromosome instability, alterations in cell viability, proliferation and apoptosis, oxidative metabolism, amyloid precursor protein and amyloid beta protein metabolism, and other cellular processes. The aim of this paper was to summarize and review the results of our investigations and the growing literature data concerning the multiple chromosomal alterations in peripheral cells of AD patients and to consider their possible role in the disease pathogenesis as well as the importance of such investigations.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika, Belgrade",
title = "Cytogenetic alterations in peripheral cells of alzheimer's disease patients",
volume = "46",
number = "1",
pages = "315-330",
doi = "10.2298/GENSR1401315P"
}

Plećaš-Solarović, B., Đelić, N., Bajić, V., Živković, L.,& Potparević, B.. (2014). Cytogenetic alterations in peripheral cells of alzheimer's disease patients. in Genetika, Belgrade
Društvo genetičara Srbije, Beograd., 46(1), 315-330.
https://doi.org/10.2298/GENSR1401315P

Plećaš-Solarović B, Đelić N, Bajić V, Živković L, Potparević B. Cytogenetic alterations in peripheral cells of alzheimer's disease patients. in Genetika, Belgrade. 2014;46(1):315-330.
doi:10.2298/GENSR1401315P .

Plećaš-Solarović, Bosiljka, Đelić, Ninoslav, Bajić, Vladan, Živković, Lada, Potparević, Biljana, "Cytogenetic alterations in peripheral cells of alzheimer's disease patients" in Genetika, Belgrade, 46, no. 1 (2014):315-330,
https://doi.org/10.2298/GENSR1401315P . .

TY  - JOUR
AU  - Živković, Lada
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Bogavac-Stanojević, Nataša
AU  - Žukovec, Dijana
AU  - Čabarkapa, Andrea
AU  - Potparević, Biljana
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1909
AB  - Background: The antioxidant activity of estrogen has a beneficial impact in Alzheimer's disease. A variety of clinical studies have demonstrated that estrogen replacement therapy in postmenopausal women results in a lower frequency of AD, delaying the onset of the neurodegenerative cascade. On the other hand, it has been demonstrated that estrogens may exhibit genotoxic effects, especially at elevated tissue concentrations. Therefore, the aim of this study was to determine whether β-estradiol induces DNA damage in the peripheral blood lymphocytes of healthy young females and males, healthy elderly females and males and females and males with Alzheimer's disease. Methods: All experiments were performed using the alkaline version of the Comet assay (single cell gel electrophoresis), on six donors per each experimental group and controls. Results: In the Comet assay, a significant increase of DNA migration was observed in the lymphocytes of all treated groups (young and elderly females, young and elderly males, AD females and AD males) at all β-estradiol concentrations (50 µmol/L, 100 µmol/L and 250 µmol/L) used in this investigation. In all the experiments cell viability was over 80%.Conclusions: Lymphocytes are sensitive to the test concentrations of β-estradiol in the Comet assay regardless of gender, age and health condition of the examined subjects. Therefore, the role of β-estradiol in cellular DNA damage has been confirmed.
AB  - Uvod: Antioksidativna svojstva estrogena imaju povoljan uticaj na Alchajmerovu bolest (AB). Brojne kliničke studije su pokazale da primena hormonske supstitucione terapije estrogenom kod žena u postmenopauzi smanjuje učestalost pojave AB, odlažući početak neurodegenerativnih procesa. S druge strane, pokazano je da estrogeni mogu ispoljiti genotoksičan efekat, naročito pri povišenim koncentracijama u tkivu. Shodno tome, cilj ove studije bio je ispitivanje sposobnosti β-estradiola da izazove oštećenja u limfocitima periferne krvi zdravih mladih žena i muškaraca, zdravih starih žena i muškaraca, kao i žena i muškaraca koji boluju od Alchajmerove bolesti. Metode: Svi eksperimenti su izvedeni primenom alkalne verzije komet testa (gel-elektroforeza DNK pojedinačnih ćelija), na po šest subjekata u svakoj ispitivanoj grupi. Rezultati: Upotrebom komet testa, zabeleženo je značajno povećanje migracije DNK u limfocitima ispitanika iz svih tretiranih grupa (mladih i starih žena, mladih i starih muškaraca kao i kod žena i muškaraca sa AB), pri svim koncentracijama β-estradiola (50 µmol/L, 100 µmol/L i 250 µmol/L) koje su korišćene u ovoj studiji. U svim eksperimentima vijabilnost ćelija je bila iznad 80%. Zaključak: Limfociti periferne krvi osetljivi su na testirane koncentracije β-estradiola, bez obzira na pol, godište i zdravstveno stanje ispitanika. U skladu s navedenim nalazima, potvrđena je uloga β-estradiola u izazivanju oštećenja na ćelijskoj DNK.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Evaluation of DNA damage in the lymphocytes of young, elderly and Alzheimer's disease patients treated with β-estradiol in the comet assay
T1  - Evaluacija oštećenja DNK u limfocitima mladih, starih i pacijenata obolelih od Alchajmerove bolesti tretiranih β-estradiolom u komet testu
VL  - 32
IS  - 3
SP  - 238
EP  - 244
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1909
ER  - 

@article{
author = "Živković, Lada and Đelić, Ninoslav and Bajić, Vladan and Bogavac-Stanojević, Nataša and Žukovec, Dijana and Čabarkapa, Andrea and Potparević, Biljana",
year = "2013",
abstract = "Background: The antioxidant activity of estrogen has a beneficial impact in Alzheimer's disease. A variety of clinical studies have demonstrated that estrogen replacement therapy in postmenopausal women results in a lower frequency of AD, delaying the onset of the neurodegenerative cascade. On the other hand, it has been demonstrated that estrogens may exhibit genotoxic effects, especially at elevated tissue concentrations. Therefore, the aim of this study was to determine whether β-estradiol induces DNA damage in the peripheral blood lymphocytes of healthy young females and males, healthy elderly females and males and females and males with Alzheimer's disease. Methods: All experiments were performed using the alkaline version of the Comet assay (single cell gel electrophoresis), on six donors per each experimental group and controls. Results: In the Comet assay, a significant increase of DNA migration was observed in the lymphocytes of all treated groups (young and elderly females, young and elderly males, AD females and AD males) at all β-estradiol concentrations (50 µmol/L, 100 µmol/L and 250 µmol/L) used in this investigation. In all the experiments cell viability was over 80%.Conclusions: Lymphocytes are sensitive to the test concentrations of β-estradiol in the Comet assay regardless of gender, age and health condition of the examined subjects. Therefore, the role of β-estradiol in cellular DNA damage has been confirmed., Uvod: Antioksidativna svojstva estrogena imaju povoljan uticaj na Alchajmerovu bolest (AB). Brojne kliničke studije su pokazale da primena hormonske supstitucione terapije estrogenom kod žena u postmenopauzi smanjuje učestalost pojave AB, odlažući početak neurodegenerativnih procesa. S druge strane, pokazano je da estrogeni mogu ispoljiti genotoksičan efekat, naročito pri povišenim koncentracijama u tkivu. Shodno tome, cilj ove studije bio je ispitivanje sposobnosti β-estradiola da izazove oštećenja u limfocitima periferne krvi zdravih mladih žena i muškaraca, zdravih starih žena i muškaraca, kao i žena i muškaraca koji boluju od Alchajmerove bolesti. Metode: Svi eksperimenti su izvedeni primenom alkalne verzije komet testa (gel-elektroforeza DNK pojedinačnih ćelija), na po šest subjekata u svakoj ispitivanoj grupi. Rezultati: Upotrebom komet testa, zabeleženo je značajno povećanje migracije DNK u limfocitima ispitanika iz svih tretiranih grupa (mladih i starih žena, mladih i starih muškaraca kao i kod žena i muškaraca sa AB), pri svim koncentracijama β-estradiola (50 µmol/L, 100 µmol/L i 250 µmol/L) koje su korišćene u ovoj studiji. U svim eksperimentima vijabilnost ćelija je bila iznad 80%. Zaključak: Limfociti periferne krvi osetljivi su na testirane koncentracije β-estradiola, bez obzira na pol, godište i zdravstveno stanje ispitanika. U skladu s navedenim nalazima, potvrđena je uloga β-estradiola u izazivanju oštećenja na ćelijskoj DNK.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Evaluation of DNA damage in the lymphocytes of young, elderly and Alzheimer's disease patients treated with β-estradiol in the comet assay, Evaluacija oštećenja DNK u limfocitima mladih, starih i pacijenata obolelih od Alchajmerove bolesti tretiranih β-estradiolom u komet testu",
volume = "32",
number = "3",
pages = "238-244",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1909"
}

Živković, L., Đelić, N., Bajić, V., Bogavac-Stanojević, N., Žukovec, D., Čabarkapa, A.,& Potparević, B.. (2013). Evaluation of DNA damage in the lymphocytes of young, elderly and Alzheimer's disease patients treated with β-estradiol in the comet assay. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 32(3), 238-244.
https://hdl.handle.net/21.15107/rcub_farfar_1909

Živković L, Đelić N, Bajić V, Bogavac-Stanojević N, Žukovec D, Čabarkapa A, Potparević B. Evaluation of DNA damage in the lymphocytes of young, elderly and Alzheimer's disease patients treated with β-estradiol in the comet assay. in Journal of Medical Biochemistry. 2013;32(3):238-244.
https://hdl.handle.net/21.15107/rcub_farfar_1909 .

Živković, Lada, Đelić, Ninoslav, Bajić, Vladan, Bogavac-Stanojević, Nataša, Žukovec, Dijana, Čabarkapa, Andrea, Potparević, Biljana, "Evaluation of DNA damage in the lymphocytes of young, elderly and Alzheimer's disease patients treated with β-estradiol in the comet assay" in Journal of Medical Biochemistry, 32, no. 3 (2013):238-244,
https://hdl.handle.net/21.15107/rcub_farfar_1909 .

TY  - CONF
AU  - Topalović, Dijana
AU  - Čabarkapa, Andrea
AU  - Živković, Lada
AU  - Đelić, Ninoslav
AU  - Dekanski, Dragana
AU  - Bajić, Vladan
AU  - Potparević, Biljana
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1876
PB  - Karger, Basel
C3  - Annals of Nutrition and Metabolism
T1  - Protective Potential of Dry Olive Leaf Extract Against Oxidative Stress in Human Lymphocytes Induced by Thyroxin
VL  - 62
IS  - Supplement 2
SP  - 51
EP  - 51
DO  - 10.1159/000351281
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_985
ER  - 

@conference{
author = "Topalović, Dijana and Čabarkapa, Andrea and Živković, Lada and Đelić, Ninoslav and Dekanski, Dragana and Bajić, Vladan and Potparević, Biljana",
year = "2013",
publisher = "Karger, Basel",
journal = "Annals of Nutrition and Metabolism",
title = "Protective Potential of Dry Olive Leaf Extract Against Oxidative Stress in Human Lymphocytes Induced by Thyroxin",
volume = "62",
number = "Supplement 2",
pages = "51-51",
doi = "10.1159/000351281",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_985"
}

Topalović, D., Čabarkapa, A., Živković, L., Đelić, N., Dekanski, D., Bajić, V.,& Potparević, B.. (2013). Protective Potential of Dry Olive Leaf Extract Against Oxidative Stress in Human Lymphocytes Induced by Thyroxin. in Annals of Nutrition and Metabolism
Karger, Basel., 62(Supplement 2), 51-51.
https://doi.org/10.1159/000351281
https://hdl.handle.net/21.15107/rcub_veterinar_985

Topalović D, Čabarkapa A, Živković L, Đelić N, Dekanski D, Bajić V, Potparević B. Protective Potential of Dry Olive Leaf Extract Against Oxidative Stress in Human Lymphocytes Induced by Thyroxin. in Annals of Nutrition and Metabolism. 2013;62(Supplement 2):51-51.
doi:10.1159/000351281
https://hdl.handle.net/21.15107/rcub_veterinar_985 .

Topalović, Dijana, Čabarkapa, Andrea, Živković, Lada, Đelić, Ninoslav, Dekanski, Dragana, Bajić, Vladan, Potparević, Biljana, "Protective Potential of Dry Olive Leaf Extract Against Oxidative Stress in Human Lymphocytes Induced by Thyroxin" in Annals of Nutrition and Metabolism, 62, no. Supplement 2 (2013):51-51,
https://doi.org/10.1159/000351281 .,
https://hdl.handle.net/21.15107/rcub_veterinar_985 .

TY  - JOUR
AU  - Radaković, Milena
AU  - Đelić, Ninoslav
AU  - Stanimirović, Zoran
AU  - Plećaš-Solarović, Bosiljka
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Bajić, Vladan
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1607
AB  - Ephedrine, a natural alkaloid from plants of the genus Ephedra, has a chemical structure similar to catecholamines. It is well established that catecholamines (adraneline, noradrenaline and dopamine) cause genotoxic and mutagenic effects. Therefore, the objectives of this investigation were to examine weather ephedrine can exhibit genotoxic effects on isolated human lymphocytes in the Comet assay. Dose-response of human lymphocytes was determined at the concentration range of ephedrine from 0.0005 μM to 500 μM. Three concentrations of ephedrine (1, 50 and 300 μM) which had acceptable cell viability (over 90%) were used for further experiments with inhibitors of DNA reparation (cytosine arabinoside and hydroxyurea). The obtained results showed that ephedrine did not induce DNA damage in isolated human lymphocytes. However, co-treatment of the negative control with DNA repair inhibitors caused a slight but significant increase of DNA damage, due to an endogenous DNA damage. Interestingly, cells treated with ephedrine and DNA repair inhibitors did not express increased DNA damage. On the basis of the obtained results it can be concluded that ephedrine did not exhibit genotoxic effects on isolated human lymphocytes. This result is in accordance with previous investigations showing negative genotoxicological results for ephedrine using bacterial gene mutation test-systems and in vitro cytogenetic analysis.
AB  - Efedrin, prirodni alkaloid iz biljaka roda Ephedra, ima sličnu hemijsku strukturu sa kateholaminima. Dobro je poznato da kateholamini (adrenalin, noradrenalin i dopamin) mogu da prouzrokuju genotoksične i mutagene efekte. Stoga su ciljevi ovog istraživanja bili da se ispita da li efedrin može da ispolji genotoksične efekte na izolovanim limfocitima čoveka u Komet testu. Odnos doza-efekat određ en je u rasponu koncentracija efedrina od 0.0005 μM do 500 μM. Tri koncentracije efedrina (1, 50 and 300 μM) koje su imale prihvatljiv nivo ćelijske vijabilnosti (preko 90%) upotrebljene su za dalje eksperimente sa inhibitorima reparacije DNK (citozin arabinozid i hidroksiurea). Dobijeni rezultati pokazuju da efedrin nije indukovao oštećenja DNK na izolovanim limfocitima čoveka. Međutim, istovremeni tretman sa inhibitorima reparacije DNK doveo je do malog ali statistički značajnog porasta oštećenja DNK kod negativne kontrole, usled endogenog oštećenja DNK. Interestantno je da ćelije tretirane sa efedrinom i inhibitorima reparacije DNK nisu ispoljile povećan nivo oštećenja DNK. Na osnovu dobijenih rezultata može se zaključiti da efedrin nije ispoljio genotoksične efekte na izolovanim limfocitima čoveka. Ovaj rezultat je u saglasnosti sa prethodnim istraživanjima u kojima je dokazano da efedrin ne dovodi do genotoksičnih efekata u bakterijskim testovima na genske mutacije i u in vitro citogenetičkim analizama.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Evaluation of the effects of ephedrine on human lymphocytes in the comet assay
T1  - Evaluacija dejstva efedrina na limfocite čoveka u komet testu
VL  - 61
IS  - 4
SP  - 363
EP  - 371
DO  - 10.2298/AVB1104363R
ER  - 

@article{
author = "Radaković, Milena and Đelić, Ninoslav and Stanimirović, Zoran and Plećaš-Solarović, Bosiljka and Spremo-Potparević, Biljana and Živković, Lada and Bajić, Vladan",
year = "2011",
abstract = "Ephedrine, a natural alkaloid from plants of the genus Ephedra, has a chemical structure similar to catecholamines. It is well established that catecholamines (adraneline, noradrenaline and dopamine) cause genotoxic and mutagenic effects. Therefore, the objectives of this investigation were to examine weather ephedrine can exhibit genotoxic effects on isolated human lymphocytes in the Comet assay. Dose-response of human lymphocytes was determined at the concentration range of ephedrine from 0.0005 μM to 500 μM. Three concentrations of ephedrine (1, 50 and 300 μM) which had acceptable cell viability (over 90%) were used for further experiments with inhibitors of DNA reparation (cytosine arabinoside and hydroxyurea). The obtained results showed that ephedrine did not induce DNA damage in isolated human lymphocytes. However, co-treatment of the negative control with DNA repair inhibitors caused a slight but significant increase of DNA damage, due to an endogenous DNA damage. Interestingly, cells treated with ephedrine and DNA repair inhibitors did not express increased DNA damage. On the basis of the obtained results it can be concluded that ephedrine did not exhibit genotoxic effects on isolated human lymphocytes. This result is in accordance with previous investigations showing negative genotoxicological results for ephedrine using bacterial gene mutation test-systems and in vitro cytogenetic analysis., Efedrin, prirodni alkaloid iz biljaka roda Ephedra, ima sličnu hemijsku strukturu sa kateholaminima. Dobro je poznato da kateholamini (adrenalin, noradrenalin i dopamin) mogu da prouzrokuju genotoksične i mutagene efekte. Stoga su ciljevi ovog istraživanja bili da se ispita da li efedrin može da ispolji genotoksične efekte na izolovanim limfocitima čoveka u Komet testu. Odnos doza-efekat određ en je u rasponu koncentracija efedrina od 0.0005 μM do 500 μM. Tri koncentracije efedrina (1, 50 and 300 μM) koje su imale prihvatljiv nivo ćelijske vijabilnosti (preko 90%) upotrebljene su za dalje eksperimente sa inhibitorima reparacije DNK (citozin arabinozid i hidroksiurea). Dobijeni rezultati pokazuju da efedrin nije indukovao oštećenja DNK na izolovanim limfocitima čoveka. Međutim, istovremeni tretman sa inhibitorima reparacije DNK doveo je do malog ali statistički značajnog porasta oštećenja DNK kod negativne kontrole, usled endogenog oštećenja DNK. Interestantno je da ćelije tretirane sa efedrinom i inhibitorima reparacije DNK nisu ispoljile povećan nivo oštećenja DNK. Na osnovu dobijenih rezultata može se zaključiti da efedrin nije ispoljio genotoksične efekte na izolovanim limfocitima čoveka. Ovaj rezultat je u saglasnosti sa prethodnim istraživanjima u kojima je dokazano da efedrin ne dovodi do genotoksičnih efekata u bakterijskim testovima na genske mutacije i u in vitro citogenetičkim analizama.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Evaluation of the effects of ephedrine on human lymphocytes in the comet assay, Evaluacija dejstva efedrina na limfocite čoveka u komet testu",
volume = "61",
number = "4",
pages = "363-371",
doi = "10.2298/AVB1104363R"
}

Radaković, M., Đelić, N., Stanimirović, Z., Plećaš-Solarović, B., Spremo-Potparević, B., Živković, L.,& Bajić, V.. (2011). Evaluation of the effects of ephedrine on human lymphocytes in the comet assay. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(4), 363-371.
https://doi.org/10.2298/AVB1104363R

Radaković M, Đelić N, Stanimirović Z, Plećaš-Solarović B, Spremo-Potparević B, Živković L, Bajić V. Evaluation of the effects of ephedrine on human lymphocytes in the comet assay. in Acta veterinaria. 2011;61(4):363-371.
doi:10.2298/AVB1104363R .

Radaković, Milena, Đelić, Ninoslav, Stanimirović, Zoran, Plećaš-Solarović, Bosiljka, Spremo-Potparević, Biljana, Živković, Lada, Bajić, Vladan, "Evaluation of the effects of ephedrine on human lymphocytes in the comet assay" in Acta veterinaria, 61, no. 4 (2011):363-371,
https://doi.org/10.2298/AVB1104363R . .

TY  - JOUR
AU  - Živković, Lada
AU  - Plećaš, Bosiljka
AU  - Bajić, Vladan
AU  - Đelić, Ninoslav
AU  - Spremo-Potparević, Biljana
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1605
AB  - DNA damage is a powerful trigger of apoptosis in neurons and is present in patients with Alzheimer's disease (AB). Also, DNA damage is registered in cells that are not neurons, such as peripheral blood leukocytes and fibroblasts. In this paper we examined and compared the impact of AB and chronological aging on DNA damage in peripheral blood leukocytes. We used electrophoresis of individual cells on gel (comet test) as a modern and reliable method for registration of nuclear DNA damage whose fragments form a comet tail, which is not present in undamaged cells. We analyzed leukocytes of these groups: AB patients with sporadic form of the disease, healthy elderly of an appropriate age and healthy younger adults as controls. The results show that the frequency of AB patients with leukocyte DNA damage significantly is higher than in similar control age-matched controls. Also, a significant increase in the frequency of DNA damage has been observed during chronological aging. Based on the obtained data we can conclude that genetic instability, otherwise present in the elderly is more expressed in patients with sporadic AB, which indicates that it is not just an epiphenomenon of aging, but is characteristic of the disease.
AB  - Oštećenja DNK su snažan okidač apoptoze neurona i prisutna su kod obolelih od Alchajmerove bolesti (AB). Ona se registruju i u ćelijama koje nisu neuroni, kao što su leukociti periferne krvi i fibroblasti. U ovom radu smo ispitivali i poredili uticaj AB i hronološkog starenja na oštećenje DNK leukocita periferne krvi. Koristili smo elektroforezu pojedinačnih ćelija na gelu (Komet test), kao savremenu i pouzdanu metodu za registrovanje oštećenja jedarne DNK čiji fragmenti formiraju rep komete, koji nije prisutan u neoštećenim ćelijama. Analizarani su leukociti: AB pacijenata sa sporadičnim oblikom bolesti, zdravih starih osoba odgovarajuće starosti i zdravih odraslih mlađih kontrolnih osoba. Dobijeni rezultati pokazuju da je kod AB ispitanika frekvencija leukocita sa oštećenjima DNK statistički značajno veća nego kod kontrola slične starosti; značajan porast učestalosti oštećenja DNK zapaža se i tokom hronološkog starenja. Na osnovu dobijenih podataka možemo da zaključimo da je genetička nestabilnost, inače prisutna i kod starih osoba, jače izražena kod pacijenata sa sporadičnom AB, što ukazuje na to da ona nije samo epifenomen starenja, već je karakteristika same bolesti.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Evaluation of DNA damages in peripheral blood leukocytes of Alzheimer's disease patients by Comet test
T1  - Ispitivanje stepena oštećenja DNK leukocita periferne krvi pacijenata sa Alchajmerovom bolešću primenom Komet testa
VL  - 61
IS  - 1
SP  - 28
EP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_795
ER  - 

@article{
author = "Živković, Lada and Plećaš, Bosiljka and Bajić, Vladan and Đelić, Ninoslav and Spremo-Potparević, Biljana",
year = "2011",
abstract = "DNA damage is a powerful trigger of apoptosis in neurons and is present in patients with Alzheimer's disease (AB). Also, DNA damage is registered in cells that are not neurons, such as peripheral blood leukocytes and fibroblasts. In this paper we examined and compared the impact of AB and chronological aging on DNA damage in peripheral blood leukocytes. We used electrophoresis of individual cells on gel (comet test) as a modern and reliable method for registration of nuclear DNA damage whose fragments form a comet tail, which is not present in undamaged cells. We analyzed leukocytes of these groups: AB patients with sporadic form of the disease, healthy elderly of an appropriate age and healthy younger adults as controls. The results show that the frequency of AB patients with leukocyte DNA damage significantly is higher than in similar control age-matched controls. Also, a significant increase in the frequency of DNA damage has been observed during chronological aging. Based on the obtained data we can conclude that genetic instability, otherwise present in the elderly is more expressed in patients with sporadic AB, which indicates that it is not just an epiphenomenon of aging, but is characteristic of the disease., Oštećenja DNK su snažan okidač apoptoze neurona i prisutna su kod obolelih od Alchajmerove bolesti (AB). Ona se registruju i u ćelijama koje nisu neuroni, kao što su leukociti periferne krvi i fibroblasti. U ovom radu smo ispitivali i poredili uticaj AB i hronološkog starenja na oštećenje DNK leukocita periferne krvi. Koristili smo elektroforezu pojedinačnih ćelija na gelu (Komet test), kao savremenu i pouzdanu metodu za registrovanje oštećenja jedarne DNK čiji fragmenti formiraju rep komete, koji nije prisutan u neoštećenim ćelijama. Analizarani su leukociti: AB pacijenata sa sporadičnim oblikom bolesti, zdravih starih osoba odgovarajuće starosti i zdravih odraslih mlađih kontrolnih osoba. Dobijeni rezultati pokazuju da je kod AB ispitanika frekvencija leukocita sa oštećenjima DNK statistički značajno veća nego kod kontrola slične starosti; značajan porast učestalosti oštećenja DNK zapaža se i tokom hronološkog starenja. Na osnovu dobijenih podataka možemo da zaključimo da je genetička nestabilnost, inače prisutna i kod starih osoba, jače izražena kod pacijenata sa sporadičnom AB, što ukazuje na to da ona nije samo epifenomen starenja, već je karakteristika same bolesti.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Evaluation of DNA damages in peripheral blood leukocytes of Alzheimer's disease patients by Comet test, Ispitivanje stepena oštećenja DNK leukocita periferne krvi pacijenata sa Alchajmerovom bolešću primenom Komet testa",
volume = "61",
number = "1",
pages = "28-41",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_795"
}

Živković, L., Plećaš, B., Bajić, V., Đelić, N.,& Spremo-Potparević, B.. (2011). Evaluation of DNA damages in peripheral blood leukocytes of Alzheimer's disease patients by Comet test. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 61(1), 28-41.
https://hdl.handle.net/21.15107/rcub_veterinar_795

Živković L, Plećaš B, Bajić V, Đelić N, Spremo-Potparević B. Evaluation of DNA damages in peripheral blood leukocytes of Alzheimer's disease patients by Comet test. in Arhiv za farmaciju. 2011;61(1):28-41.
https://hdl.handle.net/21.15107/rcub_veterinar_795 .

Živković, Lada, Plećaš, Bosiljka, Bajić, Vladan, Đelić, Ninoslav, Spremo-Potparević, Biljana, "Evaluation of DNA damages in peripheral blood leukocytes of Alzheimer's disease patients by Comet test" in Arhiv za farmaciju, 61, no. 1 (2011):28-41,
https://hdl.handle.net/21.15107/rcub_veterinar_795 .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Su, Bo
AU  - Lee, Hyoung-Gon
AU  - Kudo, Wataru
AU  - Siedlak, Sandra L.
AU  - Živković, Lada
AU  - Potparević, Biljana
AU  - Đelić, Ninoslav
AU  - Milicević, Zorana
AU  - Singh, Avneet K.
AU  - Fahmy, Lara M.
AU  - Wang, Xinglong
AU  - Smith, Mark A.
AU  - Zhu, Xiongwei
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1554
AB  - Post-mitotic neurons are typically terminally differentiated and in a quiescent status. However, in Alzheimer disease (AD), many neurons display ectopic re-expression of cell cycle-related proteins. Cyclin-dependent kinase 11 (CDK11) mRNA produces a 110-kDa protein (CDK11(p110)) throughout the cell cycle, a 58-kDa protein (CDK11(p58)) that is specifically translated from an internal ribosome entry site and expressed only in the G(2)/M phase of the cell cycle, and a 46-kDa protein (CDK11(p46)) that is considered to be apoptosis specific. CDK11 is required for sister chromatid cohesion and the completion of mitosis. In this study, we found that the expression patterns of CDK11 vary such that cytoplasmic CDK11 is increased in AD cellular processes, compared to a pronounced nuclear expression pattern in most controls. We also investigated the effect of amyloid precursor protein (APP) on CDK11 expression in vitro by using M17 cells overexpressing wild-type APP and APP Swedish mutant phenotype and found increased CDK11 expression compared to empty vector. In addition, amyloid-beta(25-35) resulted in increased CDK11 in M17 cells. These data suggest that CDK11 may play a vital role in cell cycle re-entry in AD neurons in an APP-dependent manner, thus presenting an intriguing novel function of the APP signaling pathway in AD.
PB  - BMC, LONDON
T2  - Cellular & Molecular Biology Letters
T1  - Mislocalization of CDK11/PITSLRE, a regulator of the G2/M phase of the cell cycle, in Alzheimer disease
VL  - 16
IS  - 3
SP  - 359
EP  - 372
DO  - 10.2478/s11658-011-0011-2
ER  - 

@article{
author = "Bajić, Vladan and Su, Bo and Lee, Hyoung-Gon and Kudo, Wataru and Siedlak, Sandra L. and Živković, Lada and Potparević, Biljana and Đelić, Ninoslav and Milicević, Zorana and Singh, Avneet K. and Fahmy, Lara M. and Wang, Xinglong and Smith, Mark A. and Zhu, Xiongwei",
year = "2011",
abstract = "Post-mitotic neurons are typically terminally differentiated and in a quiescent status. However, in Alzheimer disease (AD), many neurons display ectopic re-expression of cell cycle-related proteins. Cyclin-dependent kinase 11 (CDK11) mRNA produces a 110-kDa protein (CDK11(p110)) throughout the cell cycle, a 58-kDa protein (CDK11(p58)) that is specifically translated from an internal ribosome entry site and expressed only in the G(2)/M phase of the cell cycle, and a 46-kDa protein (CDK11(p46)) that is considered to be apoptosis specific. CDK11 is required for sister chromatid cohesion and the completion of mitosis. In this study, we found that the expression patterns of CDK11 vary such that cytoplasmic CDK11 is increased in AD cellular processes, compared to a pronounced nuclear expression pattern in most controls. We also investigated the effect of amyloid precursor protein (APP) on CDK11 expression in vitro by using M17 cells overexpressing wild-type APP and APP Swedish mutant phenotype and found increased CDK11 expression compared to empty vector. In addition, amyloid-beta(25-35) resulted in increased CDK11 in M17 cells. These data suggest that CDK11 may play a vital role in cell cycle re-entry in AD neurons in an APP-dependent manner, thus presenting an intriguing novel function of the APP signaling pathway in AD.",
publisher = "BMC, LONDON",
journal = "Cellular & Molecular Biology Letters",
title = "Mislocalization of CDK11/PITSLRE, a regulator of the G2/M phase of the cell cycle, in Alzheimer disease",
volume = "16",
number = "3",
pages = "359-372",
doi = "10.2478/s11658-011-0011-2"
}

Bajić, V., Su, B., Lee, H., Kudo, W., Siedlak, S. L., Živković, L., Potparević, B., Đelić, N., Milicević, Z., Singh, A. K., Fahmy, L. M., Wang, X., Smith, M. A.,& Zhu, X.. (2011). Mislocalization of CDK11/PITSLRE, a regulator of the G2/M phase of the cell cycle, in Alzheimer disease. in Cellular & Molecular Biology Letters
BMC, LONDON., 16(3), 359-372.
https://doi.org/10.2478/s11658-011-0011-2

Bajić V, Su B, Lee H, Kudo W, Siedlak SL, Živković L, Potparević B, Đelić N, Milicević Z, Singh AK, Fahmy LM, Wang X, Smith MA, Zhu X. Mislocalization of CDK11/PITSLRE, a regulator of the G2/M phase of the cell cycle, in Alzheimer disease. in Cellular & Molecular Biology Letters. 2011;16(3):359-372.
doi:10.2478/s11658-011-0011-2 .

Bajić, Vladan, Su, Bo, Lee, Hyoung-Gon, Kudo, Wataru, Siedlak, Sandra L., Živković, Lada, Potparević, Biljana, Đelić, Ninoslav, Milicević, Zorana, Singh, Avneet K., Fahmy, Lara M., Wang, Xinglong, Smith, Mark A., Zhu, Xiongwei, "Mislocalization of CDK11/PITSLRE, a regulator of the G2/M phase of the cell cycle, in Alzheimer disease" in Cellular & Molecular Biology Letters, 16, no. 3 (2011):359-372,
https://doi.org/10.2478/s11658-011-0011-2 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Potparević, Biljana
AU  - Plećaš-Solarović, Bosiljka
AU  - Đelić, Ninoslav
AU  - Ocić, Gordana
AU  - Smiljković, Predrag
AU  - Siedlak, Sandra L.
AU  - Smith, Mark A.
AU  - Bajić, Vladan
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1370
AB  - Chromosomal alterations are a feature of both aging and Alzheimer's disease (AD). This study examined if premature centromere division (PCD), a chromosomal instability indicator increased in AD, is correlated with aging or, instead, represents a de novo chromosomal alteration due to accelerating aging in AD. PCD in peripheral blood lymphocytes was determined in sporadic AD patients and gender and age-matched unaffected controls. Metaphase nuclei were analyzed for chromosomes showing PCD, X chromosomes with PCD (PCD,X), and acrocentric chromosomes showing PCD. AD patients, regardless of age, demonstrated increased PCD on any chromosome and PCD on acrocentric chromosomes in both genders, whereas an increase in frequency of PCD,X was expressed only in women. This cytogenetic analysis suggests that PCD is a feature of AD, rather than an epiphenomenon of chronological aging, and may be useful as a physiological biomarker that can be used for disease diagnosis.
PB  - Oxford Univ Press Inc, Cary
T2  - Journals of Gerontology Series A: Biological Sciences and Medical Sciences
T1  - Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer's Disease Patients: Relation to Gender and Age
VL  - 65
IS  - 12
SP  - 1269
EP  - 1274
DO  - 10.1093/gerona/glq148
ER  - 

@article{
author = "Živković, Lada and Potparević, Biljana and Plećaš-Solarović, Bosiljka and Đelić, Ninoslav and Ocić, Gordana and Smiljković, Predrag and Siedlak, Sandra L. and Smith, Mark A. and Bajić, Vladan",
year = "2010",
abstract = "Chromosomal alterations are a feature of both aging and Alzheimer's disease (AD). This study examined if premature centromere division (PCD), a chromosomal instability indicator increased in AD, is correlated with aging or, instead, represents a de novo chromosomal alteration due to accelerating aging in AD. PCD in peripheral blood lymphocytes was determined in sporadic AD patients and gender and age-matched unaffected controls. Metaphase nuclei were analyzed for chromosomes showing PCD, X chromosomes with PCD (PCD,X), and acrocentric chromosomes showing PCD. AD patients, regardless of age, demonstrated increased PCD on any chromosome and PCD on acrocentric chromosomes in both genders, whereas an increase in frequency of PCD,X was expressed only in women. This cytogenetic analysis suggests that PCD is a feature of AD, rather than an epiphenomenon of chronological aging, and may be useful as a physiological biomarker that can be used for disease diagnosis.",
publisher = "Oxford Univ Press Inc, Cary",
journal = "Journals of Gerontology Series A: Biological Sciences and Medical Sciences",
title = "Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer's Disease Patients: Relation to Gender and Age",
volume = "65",
number = "12",
pages = "1269-1274",
doi = "10.1093/gerona/glq148"
}

Živković, L., Potparević, B., Plećaš-Solarović, B., Đelić, N., Ocić, G., Smiljković, P., Siedlak, S. L., Smith, M. A.,& Bajić, V.. (2010). Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer's Disease Patients: Relation to Gender and Age. in Journals of Gerontology Series A: Biological Sciences and Medical Sciences
Oxford Univ Press Inc, Cary., 65(12), 1269-1274.
https://doi.org/10.1093/gerona/glq148

Živković L, Potparević B, Plećaš-Solarović B, Đelić N, Ocić G, Smiljković P, Siedlak SL, Smith MA, Bajić V. Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer's Disease Patients: Relation to Gender and Age. in Journals of Gerontology Series A: Biological Sciences and Medical Sciences. 2010;65(12):1269-1274.
doi:10.1093/gerona/glq148 .

Živković, Lada, Potparević, Biljana, Plećaš-Solarović, Bosiljka, Đelić, Ninoslav, Ocić, Gordana, Smiljković, Predrag, Siedlak, Sandra L., Smith, Mark A., Bajić, Vladan, "Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer's Disease Patients: Relation to Gender and Age" in Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 65, no. 12 (2010):1269-1274,
https://doi.org/10.1093/gerona/glq148 . .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Đelić, Ninoslav
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Milicević, Z.
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1072
AB  - 8-chloro-cyclic adenosine 3',5'-monophosphate (8-Cl-cAMP) is the most potent cAMP analog that selectively inhibits a variety of cancer cell lines in vitro and tumors in vivo. Its action toward a variety of tumors, especially when coupled with other antitumor agents, have lead to phase I clinical investigations and recently phase II clinical investigations. Until today, very little was done to evaluate its genotoxic potential. In order to evaluate its genotoxic potential we used the cytogenetic and cytokinesis block micronucleus assay in vitro on peripheral blood lymphocytes of healthy individuals. In three concentrations (1 mu M, 5 mu M and 15 mu M), 8-Cl-cAMP in normal human peripheral blood lymphocytes did not induce any cytogenetic aberrations of the structural type (chromatid breakage, isochromatid breakage and gaps), but did induce premature centromere separation (PCS) at all respective doses and increased the frequency of micronuclei (p  lt  0.05) only at the highest dose (15 mu M). Antiproliferative action of 8-Cl-cAMP was estimated by using the cytokinesis block nuclear division index (NDI). The results showed a decrease in NDI of cells exposed to all doses of 8-Cl-cAMP when compared to control. Therefore, the overall results show a genotoxic potential of 8-Cl-cAMP in peripheral blood lymphocytes in vitro.
PB  - Maik Nauka/Interperiodica/Springer, New York
T2  - Russian Journal of Physical Chemistry A
T1  - A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro
VL  - 44
IS  - 5
SP  - 546
EP  - 552
DO  - 10.1134/S1022795408050062
ER  - 

@article{
author = "Bajić, Vladan and Đelić, Ninoslav and Potparević, Biljana and Živković, Lada and Milicević, Z.",
year = "2008",
abstract = "8-chloro-cyclic adenosine 3',5'-monophosphate (8-Cl-cAMP) is the most potent cAMP analog that selectively inhibits a variety of cancer cell lines in vitro and tumors in vivo. Its action toward a variety of tumors, especially when coupled with other antitumor agents, have lead to phase I clinical investigations and recently phase II clinical investigations. Until today, very little was done to evaluate its genotoxic potential. In order to evaluate its genotoxic potential we used the cytogenetic and cytokinesis block micronucleus assay in vitro on peripheral blood lymphocytes of healthy individuals. In three concentrations (1 mu M, 5 mu M and 15 mu M), 8-Cl-cAMP in normal human peripheral blood lymphocytes did not induce any cytogenetic aberrations of the structural type (chromatid breakage, isochromatid breakage and gaps), but did induce premature centromere separation (PCS) at all respective doses and increased the frequency of micronuclei (p  lt  0.05) only at the highest dose (15 mu M). Antiproliferative action of 8-Cl-cAMP was estimated by using the cytokinesis block nuclear division index (NDI). The results showed a decrease in NDI of cells exposed to all doses of 8-Cl-cAMP when compared to control. Therefore, the overall results show a genotoxic potential of 8-Cl-cAMP in peripheral blood lymphocytes in vitro.",
publisher = "Maik Nauka/Interperiodica/Springer, New York",
journal = "Russian Journal of Physical Chemistry A",
title = "A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro",
volume = "44",
number = "5",
pages = "546-552",
doi = "10.1134/S1022795408050062"
}

Bajić, V., Đelić, N., Potparević, B., Živković, L.,& Milicević, Z.. (2008). A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro. in Russian Journal of Physical Chemistry A
Maik Nauka/Interperiodica/Springer, New York., 44(5), 546-552.
https://doi.org/10.1134/S1022795408050062

Bajić V, Đelić N, Potparević B, Živković L, Milicević Z. A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro. in Russian Journal of Physical Chemistry A. 2008;44(5):546-552.
doi:10.1134/S1022795408050062 .

Bajić, Vladan, Đelić, Ninoslav, Potparević, Biljana, Živković, Lada, Milicević, Z., "A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro" in Russian Journal of Physical Chemistry A, 44, no. 5 (2008):546-552,
https://doi.org/10.1134/S1022795408050062 . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Marković, Biljana
AU  - Dačić, S.
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1144
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - In vitro analysis of clastogenic effects of adrenaline on human lymphocytes
VL  - 60
IS  - 3
SP  - 15
EP  - 16
DO  - 10.2298/ABS0803015D
ER  - 

@article{
author = "Đelić, Ninoslav and Potparević, Biljana and Živković, Lada and Marković, Biljana and Dačić, S.",
year = "2008",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "In vitro analysis of clastogenic effects of adrenaline on human lymphocytes",
volume = "60",
number = "3",
pages = "15-16",
doi = "10.2298/ABS0803015D"
}

Đelić, N., Potparević, B., Živković, L., Marković, B.,& Dačić, S.. (2008). In vitro analysis of clastogenic effects of adrenaline on human lymphocytes. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 60(3), 15-16.
https://doi.org/10.2298/ABS0803015D

Đelić N, Potparević B, Živković L, Marković B, Dačić S. In vitro analysis of clastogenic effects of adrenaline on human lymphocytes. in Archives of Biological Sciences. 2008;60(3):15-16.
doi:10.2298/ABS0803015D .

Đelić, Ninoslav, Potparević, Biljana, Živković, Lada, Marković, Biljana, Dačić, S., "In vitro analysis of clastogenic effects of adrenaline on human lymphocytes" in Archives of Biological Sciences, 60, no. 3 (2008):15-16,
https://doi.org/10.2298/ABS0803015D . .

TY  - JOUR
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Đelić, Ninoslav
AU  - Plećaš-Solarović, Bosiljka
AU  - Smith, Mark A.
AU  - Bajić, Vladan
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1114
AB  - Premature centromere division (PCD) represents a loss of control over the sequential separation and segregation of chromosome centromeres. Although first described in aging women, PCD on the X chromosome (PCD,X) is markedly elevated in peripheral blood lymphocytes of individuals suffering from Alzheimer disease (AD). The present study evaluated PCD,X, using a fluorescent in situ hybridization method, in interphase nuclei of frontal cerebral cortex neurons from sporadic AD patients and age-matched controls. The average frequency of PCD,X in AD patients (8.60 +/- 1.20%) was almost three times higher (p  lt  0.01) than in the control group (2.96 +/- 1.20). However, consistent with previous studies, no mitotic cells were found in neurons in either AD or control brain, suggesting an intrinsic inability of post-mitotic neurons to divide. In view of the fact that it has been well-documented that neurons in AD can re-enter into the cell division cycle, the findings presented here of increased PCD advance the hypothesis that deregulation of the cell cycle may contribute to neuronal degeneration and subsequent cognitive deficits in AD.
PB  - Wiley-Blackwell, Malden
T2  - Journal of Neurochemistry
T1  - Premature centromere division of the X chromosome in neurons in Alzheimer's disease
VL  - 106
IS  - 5
SP  - 2218
EP  - 2223
DO  - 10.1111/j.1471-4159.2008.05555.x
ER  - 

@article{
author = "Potparević, Biljana and Živković, Lada and Đelić, Ninoslav and Plećaš-Solarović, Bosiljka and Smith, Mark A. and Bajić, Vladan",
year = "2008",
abstract = "Premature centromere division (PCD) represents a loss of control over the sequential separation and segregation of chromosome centromeres. Although first described in aging women, PCD on the X chromosome (PCD,X) is markedly elevated in peripheral blood lymphocytes of individuals suffering from Alzheimer disease (AD). The present study evaluated PCD,X, using a fluorescent in situ hybridization method, in interphase nuclei of frontal cerebral cortex neurons from sporadic AD patients and age-matched controls. The average frequency of PCD,X in AD patients (8.60 +/- 1.20%) was almost three times higher (p  lt  0.01) than in the control group (2.96 +/- 1.20). However, consistent with previous studies, no mitotic cells were found in neurons in either AD or control brain, suggesting an intrinsic inability of post-mitotic neurons to divide. In view of the fact that it has been well-documented that neurons in AD can re-enter into the cell division cycle, the findings presented here of increased PCD advance the hypothesis that deregulation of the cell cycle may contribute to neuronal degeneration and subsequent cognitive deficits in AD.",
publisher = "Wiley-Blackwell, Malden",
journal = "Journal of Neurochemistry",
title = "Premature centromere division of the X chromosome in neurons in Alzheimer's disease",
volume = "106",
number = "5",
pages = "2218-2223",
doi = "10.1111/j.1471-4159.2008.05555.x"
}

Potparević, B., Živković, L., Đelić, N., Plećaš-Solarović, B., Smith, M. A.,& Bajić, V.. (2008). Premature centromere division of the X chromosome in neurons in Alzheimer's disease. in Journal of Neurochemistry
Wiley-Blackwell, Malden., 106(5), 2218-2223.
https://doi.org/10.1111/j.1471-4159.2008.05555.x

Potparević B, Živković L, Đelić N, Plećaš-Solarović B, Smith MA, Bajić V. Premature centromere division of the X chromosome in neurons in Alzheimer's disease. in Journal of Neurochemistry. 2008;106(5):2218-2223.
doi:10.1111/j.1471-4159.2008.05555.x .

Potparević, Biljana, Živković, Lada, Đelić, Ninoslav, Plećaš-Solarović, Bosiljka, Smith, Mark A., Bajić, Vladan, "Premature centromere division of the X chromosome in neurons in Alzheimer's disease" in Journal of Neurochemistry, 106, no. 5 (2008):2218-2223,
https://doi.org/10.1111/j.1471-4159.2008.05555.x . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Đelić, Dijana J.
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Marković, Biljana
AU  - Lozance, Olivera
AU  - Blagojević, Miloš
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/901
AB  - Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 mu M to 50 mu M of L-thyroxine for 24 h and 48 h, these concentrations being chosen because they had been used in previous studies of sister-chromatid exchange and micronuclei frequency. Under our experimental conditions thyroxine did not induced any statistically significant increase in chromosome breakage or aberrations. This lack of clastogenic effects is in contrast to the reported comet assay results obtained using purified lymphocytes, possibly because whole-blood cultures contain catalase and glutathione peroxidase capable of reducing the effects of reactive oxygen species.
PB  - Soc Brasil Genetica, Ribeirao Pret
T2  - Genetics and Molecular Biology
T1  - Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes
VL  - 30
IS  - 4
SP  - 1144
EP  - 1149
DO  - 10.1590/S1415-47572007000600019
ER  - 

@article{
author = "Đelić, Ninoslav and Đelić, Dijana J. and Potparević, Biljana and Živković, Lada and Marković, Biljana and Lozance, Olivera and Blagojević, Miloš",
year = "2007",
abstract = "Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 mu M to 50 mu M of L-thyroxine for 24 h and 48 h, these concentrations being chosen because they had been used in previous studies of sister-chromatid exchange and micronuclei frequency. Under our experimental conditions thyroxine did not induced any statistically significant increase in chromosome breakage or aberrations. This lack of clastogenic effects is in contrast to the reported comet assay results obtained using purified lymphocytes, possibly because whole-blood cultures contain catalase and glutathione peroxidase capable of reducing the effects of reactive oxygen species.",
publisher = "Soc Brasil Genetica, Ribeirao Pret",
journal = "Genetics and Molecular Biology",
title = "Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes",
volume = "30",
number = "4",
pages = "1144-1149",
doi = "10.1590/S1415-47572007000600019"
}

Đelić, N., Đelić, D. J., Potparević, B., Živković, L., Marković, B., Lozance, O.,& Blagojević, M.. (2007). Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes. in Genetics and Molecular Biology
Soc Brasil Genetica, Ribeirao Pret., 30(4), 1144-1149.
https://doi.org/10.1590/S1415-47572007000600019

Đelić N, Đelić DJ, Potparević B, Živković L, Marković B, Lozance O, Blagojević M. Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes. in Genetics and Molecular Biology. 2007;30(4):1144-1149.
doi:10.1590/S1415-47572007000600019 .

Đelić, Ninoslav, Đelić, Dijana J., Potparević, Biljana, Živković, Lada, Marković, Biljana, Lozance, Olivera, Blagojević, Miloš, "Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes" in Genetics and Molecular Biology, 30, no. 4 (2007):1144-1149,
https://doi.org/10.1590/S1415-47572007000600019 . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Spremo-Potparević, Biljana
AU  - Marković, Biljana
AU  - Živković, Lada
AU  - Đelić, Dijana J.
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/868
AB  - Metabolic conversion of oestrogen phenolic groups may create conditions of oxidative stress accompanied by damage of cellular macromolecules including DNA. The aim of this investigation was to evaluate the cell cycle kinetics and possible cytogenetic changes in cultured human peripheral blood lymphocytes exposed to seven experimental concentrations of 17β-oestradiol (range 10-10 M to 10-4 M). Cell cycle kinetics was analyzed on metaphase spreads prepared for a standard analysis of sister-chromatid exchanges (SCEs) stained by fluorescent-plus-Giemsa (FPG) technique. Cytogenetic changes were monitored by analysis of chromosome damage (gaps and breaks), structural and numerical aberrations. On the basis of the obtained results it can be concluded that oestradiol has no significant influence on cell cycle kinetics and mitotic index of cultured human lymphocytes. However, at estradiol concentration of 7×10-6 M, and at higher concentrations used in this experiment, there was a significant increase of gaps, breaks and aneuploidies. On the other hand, oestradiol treatment has not changed the frequency of polyploid cells. Therefore, it can be concluded that high concentrations of oestradiol pose some genetic risk detectable at cytogenetic level.
AB  - Metabolička konverzija fenolnih grupa estrogenih hormona može da dovede do oksidativnog stresa praćenog oštećenjima različitih makromolekula u eliji, uključujući DNK. Cilj ovog istraživanja je evaluacija kinetike proliferacije i mogućih citogenetičkih promena u kulturama humanih limfocita pod dejstvom sedam eksperimentalnih koncentracija 17β-estradiola (opseg od 10-10M do10-4 M). Kinetika proliferacije limfocita analizirana je na metafaznim figurama obojenim tehnikom FPG za standardne analize razmena sestrinskih hromatida (SCE). Citogenetičke promene praćene su analizama hromozomskih oštećenja (gapovi i prekidi), strukturnih i numeričkih aberacija hromozoma. Na osnovu dobijenih rezultata može se zaključiti da estradiol ne utiče značajno na mitotsku aktivnost i kinetiku proliferacije limfocita u kulturi. Međutim, pri koncentraciji od 7×10-6 M, kao i pri višim eksperimentalnim koncentracijama korišćenim u ovim eksperimentima, zapažen je porast gapova, prekida i aneuploidija. S druge strane, tretman estradiolom ne menja učestalost poliploidnih ćelija. Prema tome, može se zaključiti da visoke koncentracije estradiola izazivaju izvestan genetički rizik koji se može detektovati na citogenetičkom nivou.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro
T1  - Kinetika proliferacije i citogenetičke promene u humanim limfocitima pod dejstvom estradiola in vitro
VL  - 56
IS  - 1
SP  - 37
EP  - 48
DO  - 10.2298/AVB0601037D
ER  - 

@article{
author = "Đelić, Ninoslav and Spremo-Potparević, Biljana and Marković, Biljana and Živković, Lada and Đelić, Dijana J.",
year = "2006",
abstract = "Metabolic conversion of oestrogen phenolic groups may create conditions of oxidative stress accompanied by damage of cellular macromolecules including DNA. The aim of this investigation was to evaluate the cell cycle kinetics and possible cytogenetic changes in cultured human peripheral blood lymphocytes exposed to seven experimental concentrations of 17β-oestradiol (range 10-10 M to 10-4 M). Cell cycle kinetics was analyzed on metaphase spreads prepared for a standard analysis of sister-chromatid exchanges (SCEs) stained by fluorescent-plus-Giemsa (FPG) technique. Cytogenetic changes were monitored by analysis of chromosome damage (gaps and breaks), structural and numerical aberrations. On the basis of the obtained results it can be concluded that oestradiol has no significant influence on cell cycle kinetics and mitotic index of cultured human lymphocytes. However, at estradiol concentration of 7×10-6 M, and at higher concentrations used in this experiment, there was a significant increase of gaps, breaks and aneuploidies. On the other hand, oestradiol treatment has not changed the frequency of polyploid cells. Therefore, it can be concluded that high concentrations of oestradiol pose some genetic risk detectable at cytogenetic level., Metabolička konverzija fenolnih grupa estrogenih hormona može da dovede do oksidativnog stresa praćenog oštećenjima različitih makromolekula u eliji, uključujući DNK. Cilj ovog istraživanja je evaluacija kinetike proliferacije i mogućih citogenetičkih promena u kulturama humanih limfocita pod dejstvom sedam eksperimentalnih koncentracija 17β-estradiola (opseg od 10-10M do10-4 M). Kinetika proliferacije limfocita analizirana je na metafaznim figurama obojenim tehnikom FPG za standardne analize razmena sestrinskih hromatida (SCE). Citogenetičke promene praćene su analizama hromozomskih oštećenja (gapovi i prekidi), strukturnih i numeričkih aberacija hromozoma. Na osnovu dobijenih rezultata može se zaključiti da estradiol ne utiče značajno na mitotsku aktivnost i kinetiku proliferacije limfocita u kulturi. Međutim, pri koncentraciji od 7×10-6 M, kao i pri višim eksperimentalnim koncentracijama korišćenim u ovim eksperimentima, zapažen je porast gapova, prekida i aneuploidija. S druge strane, tretman estradiolom ne menja učestalost poliploidnih ćelija. Prema tome, može se zaključiti da visoke koncentracije estradiola izazivaju izvestan genetički rizik koji se može detektovati na citogenetičkom nivou.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro, Kinetika proliferacije i citogenetičke promene u humanim limfocitima pod dejstvom estradiola in vitro",
volume = "56",
number = "1",
pages = "37-48",
doi = "10.2298/AVB0601037D"
}

Đelić, N., Spremo-Potparević, B., Marković, B., Živković, L.,& Đelić, D. J.. (2006). Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 56(1), 37-48.
https://doi.org/10.2298/AVB0601037D

Đelić N, Spremo-Potparević B, Marković B, Živković L, Đelić DJ. Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro. in Acta veterinaria. 2006;56(1):37-48.
doi:10.2298/AVB0601037D .

Đelić, Ninoslav, Spremo-Potparević, Biljana, Marković, Biljana, Živković, Lada, Đelić, Dijana J., "Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro" in Acta veterinaria, 56, no. 1 (2006):37-48,
https://doi.org/10.2298/AVB0601037D . .

TY  - CONF
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Đelić, Ninoslav
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/753
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Flow-cytometric analysis of the cell cycle in peripheral blood lymphocytes exposed to antitumor agents 8-Cl-cAMP, Taxol and Mitomycin C with and without the induction of PCS
T1  - Protočno-citometrijaska analiza ćelijskog ciklusa limfocita periferne krvi eksponiranih antitumorskim agensima 8-Cl-cAMP, Taxol i Mitomycin C sa i bez indukcije PCS
VL  - 56
IS  - 4
SP  - 386
EP  - 387
UR  - https://hdl.handle.net/21.15107/rcub_farfar_753
ER  - 

@conference{
author = "Bajić, Vladan and Spremo-Potparević, Biljana and Živković, Lada and Đelić, Ninoslav",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Flow-cytometric analysis of the cell cycle in peripheral blood lymphocytes exposed to antitumor agents 8-Cl-cAMP, Taxol and Mitomycin C with and without the induction of PCS, Protočno-citometrijaska analiza ćelijskog ciklusa limfocita periferne krvi eksponiranih antitumorskim agensima 8-Cl-cAMP, Taxol i Mitomycin C sa i bez indukcije PCS",
volume = "56",
number = "4",
pages = "386-387",
url = "https://hdl.handle.net/21.15107/rcub_farfar_753"
}

Bajić, V., Spremo-Potparević, B., Živković, L.,& Đelić, N.. (2006). Flow-cytometric analysis of the cell cycle in peripheral blood lymphocytes exposed to antitumor agents 8-Cl-cAMP, Taxol and Mitomycin C with and without the induction of PCS. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(4), 386-387.
https://hdl.handle.net/21.15107/rcub_farfar_753

Bajić V, Spremo-Potparević B, Živković L, Đelić N. Flow-cytometric analysis of the cell cycle in peripheral blood lymphocytes exposed to antitumor agents 8-Cl-cAMP, Taxol and Mitomycin C with and without the induction of PCS. in Arhiv za farmaciju. 2006;56(4):386-387.
https://hdl.handle.net/21.15107/rcub_farfar_753 .

Bajić, Vladan, Spremo-Potparević, Biljana, Živković, Lada, Đelić, Ninoslav, "Flow-cytometric analysis of the cell cycle in peripheral blood lymphocytes exposed to antitumor agents 8-Cl-cAMP, Taxol and Mitomycin C with and without the induction of PCS" in Arhiv za farmaciju, 56, no. 4 (2006):386-387,
https://hdl.handle.net/21.15107/rcub_farfar_753 .