TY  - JOUR
AU  - Li, Lihong
AU  - Zou, Tengteng
AU  - Liang, Min
AU  - Mezhuev, Yaroslav
AU  - Tsatsakis, Aristidis Michael
AU  - Buha-Đorđević, Aleksandra
AU  - Lan, Meng
AU  - Liu, Fengjie
AU  - Cai, Tiange
AU  - Gong, Peng
AU  - Cai, Yu
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3911
AB  - Objective: Our study aimed to find potential biomarkers for drug resistance in liver cancer cells using metabolomics and further to evaluate the potential of psoralen-loaded polymer lipid nanoparticles (PSO-PLNs) to reverse the resistance of cells to doxorubicin. Methods: We used LC-MS-based non-targeted metabolomics, also known as global metabolite profiling, to screen in serum and urine of mice engrafted with a liver cancer cell line sensitive (HepG2/S) or resistant to doxorubicin (HepG2/ADR) for differentially regulated metabolites. We subsequently quantified the abundance of these metabolites in serum and the urine of mice. The mice were engrafted with HepG2 cells resistant against doxorubicin and were treated with I) doxorubicin, II) a combination of doxorubicin and psoralen and III) a combination of doxorubicin and psoralen packed in polymer lipid nanoparticles. Results: Metabolites found to be differentially present in urine of mice engrafted with resistant HepG2 cells were: hippuric acid, hyaluronic acid, pantothenic acid, and betaine; retinoic acid and α-linolenic acid were found to be reduced in serum samples of mice with HepG2 cells resistant to doxorubicin. The targeted analysis showed that the degree of regression of metabolic markers in groups differed: treatment group 2 had stronger degree of regression than treatment group 1 and the negative control group had the smallest, which indicates that the PSO-PLNs have superior properties compared with other treatments. Conclusion: Psoralen reverses drug resistance of liver cancer cells and its efficacy can be increased by encapsulation in polymer lipid nanoparticles.
PB  - Elsevier B.V.
T2  - European Journal of Pharmaceutics and Biopharmaceutics
T1  - Screening of metabolites in the treatment of liver cancer xenografts HepG2/ADR by psoralen-loaded lipid nanoparticles
VL  - 165
SP  - 337
EP  - 344
DO  - 10.1016/j.ejpb.2021.05.025
ER  - 

@article{
author = "Li, Lihong and Zou, Tengteng and Liang, Min and Mezhuev, Yaroslav and Tsatsakis, Aristidis Michael and Buha-Đorđević, Aleksandra and Lan, Meng and Liu, Fengjie and Cai, Tiange and Gong, Peng and Cai, Yu",
year = "2021",
abstract = "Objective: Our study aimed to find potential biomarkers for drug resistance in liver cancer cells using metabolomics and further to evaluate the potential of psoralen-loaded polymer lipid nanoparticles (PSO-PLNs) to reverse the resistance of cells to doxorubicin. Methods: We used LC-MS-based non-targeted metabolomics, also known as global metabolite profiling, to screen in serum and urine of mice engrafted with a liver cancer cell line sensitive (HepG2/S) or resistant to doxorubicin (HepG2/ADR) for differentially regulated metabolites. We subsequently quantified the abundance of these metabolites in serum and the urine of mice. The mice were engrafted with HepG2 cells resistant against doxorubicin and were treated with I) doxorubicin, II) a combination of doxorubicin and psoralen and III) a combination of doxorubicin and psoralen packed in polymer lipid nanoparticles. Results: Metabolites found to be differentially present in urine of mice engrafted with resistant HepG2 cells were: hippuric acid, hyaluronic acid, pantothenic acid, and betaine; retinoic acid and α-linolenic acid were found to be reduced in serum samples of mice with HepG2 cells resistant to doxorubicin. The targeted analysis showed that the degree of regression of metabolic markers in groups differed: treatment group 2 had stronger degree of regression than treatment group 1 and the negative control group had the smallest, which indicates that the PSO-PLNs have superior properties compared with other treatments. Conclusion: Psoralen reverses drug resistance of liver cancer cells and its efficacy can be increased by encapsulation in polymer lipid nanoparticles.",
publisher = "Elsevier B.V.",
journal = "European Journal of Pharmaceutics and Biopharmaceutics",
title = "Screening of metabolites in the treatment of liver cancer xenografts HepG2/ADR by psoralen-loaded lipid nanoparticles",
volume = "165",
pages = "337-344",
doi = "10.1016/j.ejpb.2021.05.025"
}

Li, L., Zou, T., Liang, M., Mezhuev, Y., Tsatsakis, A. M., Buha-Đorđević, A., Lan, M., Liu, F., Cai, T., Gong, P.,& Cai, Y.. (2021). Screening of metabolites in the treatment of liver cancer xenografts HepG2/ADR by psoralen-loaded lipid nanoparticles. in European Journal of Pharmaceutics and Biopharmaceutics
Elsevier B.V.., 165, 337-344.
https://doi.org/10.1016/j.ejpb.2021.05.025

Li L, Zou T, Liang M, Mezhuev Y, Tsatsakis AM, Buha-Đorđević A, Lan M, Liu F, Cai T, Gong P, Cai Y. Screening of metabolites in the treatment of liver cancer xenografts HepG2/ADR by psoralen-loaded lipid nanoparticles. in European Journal of Pharmaceutics and Biopharmaceutics. 2021;165:337-344.
doi:10.1016/j.ejpb.2021.05.025 .

Li, Lihong, Zou, Tengteng, Liang, Min, Mezhuev, Yaroslav, Tsatsakis, Aristidis Michael, Buha-Đorđević, Aleksandra, Lan, Meng, Liu, Fengjie, Cai, Tiange, Gong, Peng, Cai, Yu, "Screening of metabolites in the treatment of liver cancer xenografts HepG2/ADR by psoralen-loaded lipid nanoparticles" in European Journal of Pharmaceutics and Biopharmaceutics, 165 (2021):337-344,
https://doi.org/10.1016/j.ejpb.2021.05.025 . .

TY  - JOUR
AU  - Varmazyari, Atefeh
AU  - Taghizadehghalehjoughi, Ali
AU  - Sevim, Cigdem
AU  - Baris, Ozlem
AU  - Eser, Gizem
AU  - Yildirim, Serkan
AU  - Hacimuftuoglu, Ahmet
AU  - Buha, Aleksandra
AU  - Wallace, David R.
AU  - Tsatsakis, Aristidis
AU  - Aschner, Michael
AU  - Mezhuev, Yaroslav
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3593
AB  - Living organisms have an innate ability to regulate the synthesis of inorganic materials, such as bones and teeth in humans. Cadmium sulfide (CdS) can be utilized as a quantum dot that functions as a unique light-emitting semiconductor nanocrystal. The increased use in CdS has led to an increased inhalation and ingestion rate of CdS by humans which requires a broader appreciation for the acute and chronic toxicity of CdS. We investigated the toxic effects of CdS on cerebellar cell cultures and rat brain. We employed a ‘green synthesis’ biosynthesis process to obtain biocompatible material that can be used in living organisms, such as Viridibacillus arenosi K64. Nanocrystal formation was initiated by adding CdCl2 (1 mM) to the cell cultures. Our in vitro results established that increased concentrations of CdS (0.1 μg/mL) lead to decreased cell viability as assessed using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT), total antioxidant capacity (TAC), and total oxidant status (TOS). The in vivo studies showed that exposure to CdS (1 mg/kg) glial fibrillary acidic protein (GFAP) and 8-hydroxy-2' -deoxyguanosine (8-OHdG) were increased. Collectively, we describe a model system that addresses the process from the synthesis to the neurotoxicity assessment for CdS both in vitro and in vivo. These data will be beneficial in establishing a more comprehensive pathway for the understanding of quantum dot-induced neurotoxicity.
PB  - Elsevier
T2  - Toxicology Reports
T1  - Cadmium sulfide-induced toxicity in the cortex and cerebellum: In vitro and in vivo studies
VL  - 7
SP  - 637
EP  - 648
DO  - 10.1016/j.toxrep.2020.04.011
ER  - 

@article{
author = "Varmazyari, Atefeh and Taghizadehghalehjoughi, Ali and Sevim, Cigdem and Baris, Ozlem and Eser, Gizem and Yildirim, Serkan and Hacimuftuoglu, Ahmet and Buha, Aleksandra and Wallace, David R. and Tsatsakis, Aristidis and Aschner, Michael and Mezhuev, Yaroslav",
year = "2020",
abstract = "Living organisms have an innate ability to regulate the synthesis of inorganic materials, such as bones and teeth in humans. Cadmium sulfide (CdS) can be utilized as a quantum dot that functions as a unique light-emitting semiconductor nanocrystal. The increased use in CdS has led to an increased inhalation and ingestion rate of CdS by humans which requires a broader appreciation for the acute and chronic toxicity of CdS. We investigated the toxic effects of CdS on cerebellar cell cultures and rat brain. We employed a ‘green synthesis’ biosynthesis process to obtain biocompatible material that can be used in living organisms, such as Viridibacillus arenosi K64. Nanocrystal formation was initiated by adding CdCl2 (1 mM) to the cell cultures. Our in vitro results established that increased concentrations of CdS (0.1 μg/mL) lead to decreased cell viability as assessed using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT), total antioxidant capacity (TAC), and total oxidant status (TOS). The in vivo studies showed that exposure to CdS (1 mg/kg) glial fibrillary acidic protein (GFAP) and 8-hydroxy-2' -deoxyguanosine (8-OHdG) were increased. Collectively, we describe a model system that addresses the process from the synthesis to the neurotoxicity assessment for CdS both in vitro and in vivo. These data will be beneficial in establishing a more comprehensive pathway for the understanding of quantum dot-induced neurotoxicity.",
publisher = "Elsevier",
journal = "Toxicology Reports",
title = "Cadmium sulfide-induced toxicity in the cortex and cerebellum: In vitro and in vivo studies",
volume = "7",
pages = "637-648",
doi = "10.1016/j.toxrep.2020.04.011"
}

Varmazyari, A., Taghizadehghalehjoughi, A., Sevim, C., Baris, O., Eser, G., Yildirim, S., Hacimuftuoglu, A., Buha, A., Wallace, D. R., Tsatsakis, A., Aschner, M.,& Mezhuev, Y.. (2020). Cadmium sulfide-induced toxicity in the cortex and cerebellum: In vitro and in vivo studies. in Toxicology Reports
Elsevier., 7, 637-648.
https://doi.org/10.1016/j.toxrep.2020.04.011

Varmazyari A, Taghizadehghalehjoughi A, Sevim C, Baris O, Eser G, Yildirim S, Hacimuftuoglu A, Buha A, Wallace DR, Tsatsakis A, Aschner M, Mezhuev Y. Cadmium sulfide-induced toxicity in the cortex and cerebellum: In vitro and in vivo studies. in Toxicology Reports. 2020;7:637-648.
doi:10.1016/j.toxrep.2020.04.011 .

Varmazyari, Atefeh, Taghizadehghalehjoughi, Ali, Sevim, Cigdem, Baris, Ozlem, Eser, Gizem, Yildirim, Serkan, Hacimuftuoglu, Ahmet, Buha, Aleksandra, Wallace, David R., Tsatsakis, Aristidis, Aschner, Michael, Mezhuev, Yaroslav, "Cadmium sulfide-induced toxicity in the cortex and cerebellum: In vitro and in vivo studies" in Toxicology Reports, 7 (2020):637-648,
https://doi.org/10.1016/j.toxrep.2020.04.011 . .