TY  - JOUR
AU  - Cilović Kozarević, Ermina
AU  - Dautović, Esmeralda
AU  - Halilčević, Dalila
AU  - Kolarević, Lamija
AU  - Šarić-Kundalić, Broza
AU  - Karić, Enida
AU  - Smajlagić, Aida
AU  - Husejnagić, Darja
AU  - Horozić, Emir
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Arsenijević, Jelena
AU  - Maksimović, Zoran
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5635
AB  - The chemical composition of Pulicaria dysenterica (L.) Bernh. aerial parts essential oil (EO), growing wild in Bosnia and Herzegovina, was presented in the study. In addition to the EO composition, antimicrobial and cytotoxic activities were also tested. The aerial parts of P. dysenterica contained 0.3% of yellow, liquid, fragrant EO. The 51 components identified accounted for 81.09% of the oil. The EO was characterized by the presence of a high concentration of oxygenated sesquiterpenes 51.83% while oxygenated monoterpenes constituted 15.57%, sesquiterpene hydrocarbons 9.32% and non-terpene compounds presented 4.37% of the EO. The dominant compounds were the sesquiterpenes caryophyllene oxide, (E)-nerolidol, β-caryophyllene and monoterpene nerol. The antimicrobial activity of EO was tested against selected ATCC strains of microorganisms, the bacteria Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli, and the fungus Candida albicans. The results showed that the investigated EO inhibited the growth of all selected ATCC strains of microorganisms. The best result was obtained against Escherichia coli bacteria with MIC value of 1 mgmL-1. The cytotoxicity of EO was measured against the HeLa cell line using the MTT method with IC50 of 188.52 μgmL-1. This study has provided scientific baseline data on the therapeutic properties of P. dysenterica.
PB  - Faculty of Technology, University in Tuzla
T2  - Technologica Acta
T1  - Antimicrobial and Cytotoxic Activities of Essential Oil from the Aerial Parts of Pulicaria dysenterica (L.) Bernh. (Asteraceae)
VL  - 16
IS  - 2
SP  - 81
EP  - 86
DO  - 10.51558/2232-7568.2023.16.2.81
ER  - 

@article{
author = "Cilović Kozarević, Ermina and Dautović, Esmeralda and Halilčević, Dalila and Kolarević, Lamija and Šarić-Kundalić, Broza and Karić, Enida and Smajlagić, Aida and Husejnagić, Darja and Horozić, Emir and Glamočlija, Jasmina and Soković, Marina and Arsenijević, Jelena and Maksimović, Zoran",
year = "2024",
abstract = "The chemical composition of Pulicaria dysenterica (L.) Bernh. aerial parts essential oil (EO), growing wild in Bosnia and Herzegovina, was presented in the study. In addition to the EO composition, antimicrobial and cytotoxic activities were also tested. The aerial parts of P. dysenterica contained 0.3% of yellow, liquid, fragrant EO. The 51 components identified accounted for 81.09% of the oil. The EO was characterized by the presence of a high concentration of oxygenated sesquiterpenes 51.83% while oxygenated monoterpenes constituted 15.57%, sesquiterpene hydrocarbons 9.32% and non-terpene compounds presented 4.37% of the EO. The dominant compounds were the sesquiterpenes caryophyllene oxide, (E)-nerolidol, β-caryophyllene and monoterpene nerol. The antimicrobial activity of EO was tested against selected ATCC strains of microorganisms, the bacteria Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli, and the fungus Candida albicans. The results showed that the investigated EO inhibited the growth of all selected ATCC strains of microorganisms. The best result was obtained against Escherichia coli bacteria with MIC value of 1 mgmL-1. The cytotoxicity of EO was measured against the HeLa cell line using the MTT method with IC50 of 188.52 μgmL-1. This study has provided scientific baseline data on the therapeutic properties of P. dysenterica.",
publisher = "Faculty of Technology, University in Tuzla",
journal = "Technologica Acta",
title = "Antimicrobial and Cytotoxic Activities of Essential Oil from the Aerial Parts of Pulicaria dysenterica (L.) Bernh. (Asteraceae)",
volume = "16",
number = "2",
pages = "81-86",
doi = "10.51558/2232-7568.2023.16.2.81"
}

Cilović Kozarević, E., Dautović, E., Halilčević, D., Kolarević, L., Šarić-Kundalić, B., Karić, E., Smajlagić, A., Husejnagić, D., Horozić, E., Glamočlija, J., Soković, M., Arsenijević, J.,& Maksimović, Z.. (2024). Antimicrobial and Cytotoxic Activities of Essential Oil from the Aerial Parts of Pulicaria dysenterica (L.) Bernh. (Asteraceae). in Technologica Acta
Faculty of Technology, University in Tuzla., 16(2), 81-86.
https://doi.org/10.51558/2232-7568.2023.16.2.81

Cilović Kozarević E, Dautović E, Halilčević D, Kolarević L, Šarić-Kundalić B, Karić E, Smajlagić A, Husejnagić D, Horozić E, Glamočlija J, Soković M, Arsenijević J, Maksimović Z. Antimicrobial and Cytotoxic Activities of Essential Oil from the Aerial Parts of Pulicaria dysenterica (L.) Bernh. (Asteraceae). in Technologica Acta. 2024;16(2):81-86.
doi:10.51558/2232-7568.2023.16.2.81 .

Cilović Kozarević, Ermina, Dautović, Esmeralda, Halilčević, Dalila, Kolarević, Lamija, Šarić-Kundalić, Broza, Karić, Enida, Smajlagić, Aida, Husejnagić, Darja, Horozić, Emir, Glamočlija, Jasmina, Soković, Marina, Arsenijević, Jelena, Maksimović, Zoran, "Antimicrobial and Cytotoxic Activities of Essential Oil from the Aerial Parts of Pulicaria dysenterica (L.) Bernh. (Asteraceae)" in Technologica Acta, 16, no. 2 (2024):81-86,
https://doi.org/10.51558/2232-7568.2023.16.2.81 . .

TY  - JOUR
AU  - Kozarević Cilović, Emina
AU  - Dautović, Esmeralda
AU  - Halilčević, Dalila
AU  - Softić, Adaleta
AU  - Srabović, Nahida
AU  - Šarić-Kundalić, Broza
AU  - Delić, Nina
AU  - Kolarević, Lamija
AU  - Mekić, Lejla
AU  - Ibišević, Merima
AU  - Horozić, Emir
AU  - Arsenijević, Jelena
AU  - Maksimović, Zoran
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4624
AB  - Aims: The aims of the study were to analyse the polyphenols of Pulicaria dysenterica (L.) Bernh. methanolic extracts from aerial and underground parts, assessment of antioxidant activity and to evaluate their cytotoxicity on HeLa cells of cervical cancer.

Methodology: The total phenolic content (TPC) of extracts was determined by the Folin-Ciocalteu spectrophotometric method. The qualitative and quantitative analysis of individual polyphenolic compounds were performed by the reverse phase HPLC method. The antioxidant capacity was evaluated by both, 2,2-diphenyl1-picrylhydrazyl radical and FRAP assay, while cytotoxicity of the extracts was assessed by MTT assay.

Results: TPC of the samples were 127.62±2.22 and 244.12±8.84 mg gallic acid equivalent/g extract. In the extracts chlorogenic acid in amount of 10.06±0.96 and 11.32±0.28 mg/g, flavonoid rutin in amount of 5.68±0.13 mg/g and three caffeic acid derivatives were recorded. Extract from underground parts achieved better antioxidant activity with IC50 value 55.36±0.75 µg/mL and FRAP value 2411.12±37.22 µmol Fe2+g-1 compared to the one from aerial parts. Extract from aerial parts achieved better cytotoxic activity with 50% inhibition of viability (IC50) at concentration of 0.389±0.07 mg/mL, against HeLa cells, compared to the extract from underground parts.

Conclusion: Analyzed Pulicaria dysenterica extracts contained phenolic acids and flavonoids. The extracts showed good antioxidant activity and cytotoxic properties against HeLa cells in vitro.
PB  - International Research Journal of Pure and Applied Chemistry
T2  - International Research Journal of Pure and Applied Chemistry
T1  - Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts
VL  - 23
IS  - 5
SP  - 23
EP  - 32
DO  - 10.9734/irjpac/2022/v23i5788
ER  - 

@article{
author = "Kozarević Cilović, Emina and Dautović, Esmeralda and Halilčević, Dalila and Softić, Adaleta and Srabović, Nahida and Šarić-Kundalić, Broza and Delić, Nina and Kolarević, Lamija and Mekić, Lejla and Ibišević, Merima and Horozić, Emir and Arsenijević, Jelena and Maksimović, Zoran",
year = "2022",
abstract = "Aims: The aims of the study were to analyse the polyphenols of Pulicaria dysenterica (L.) Bernh. methanolic extracts from aerial and underground parts, assessment of antioxidant activity and to evaluate their cytotoxicity on HeLa cells of cervical cancer.

Methodology: The total phenolic content (TPC) of extracts was determined by the Folin-Ciocalteu spectrophotometric method. The qualitative and quantitative analysis of individual polyphenolic compounds were performed by the reverse phase HPLC method. The antioxidant capacity was evaluated by both, 2,2-diphenyl1-picrylhydrazyl radical and FRAP assay, while cytotoxicity of the extracts was assessed by MTT assay.

Results: TPC of the samples were 127.62±2.22 and 244.12±8.84 mg gallic acid equivalent/g extract. In the extracts chlorogenic acid in amount of 10.06±0.96 and 11.32±0.28 mg/g, flavonoid rutin in amount of 5.68±0.13 mg/g and three caffeic acid derivatives were recorded. Extract from underground parts achieved better antioxidant activity with IC50 value 55.36±0.75 µg/mL and FRAP value 2411.12±37.22 µmol Fe2+g-1 compared to the one from aerial parts. Extract from aerial parts achieved better cytotoxic activity with 50% inhibition of viability (IC50) at concentration of 0.389±0.07 mg/mL, against HeLa cells, compared to the extract from underground parts.

Conclusion: Analyzed Pulicaria dysenterica extracts contained phenolic acids and flavonoids. The extracts showed good antioxidant activity and cytotoxic properties against HeLa cells in vitro.",
publisher = "International Research Journal of Pure and Applied Chemistry",
journal = "International Research Journal of Pure and Applied Chemistry",
title = "Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts",
volume = "23",
number = "5",
pages = "23-32",
doi = "10.9734/irjpac/2022/v23i5788"
}

Kozarević Cilović, E., Dautović, E., Halilčević, D., Softić, A., Srabović, N., Šarić-Kundalić, B., Delić, N., Kolarević, L., Mekić, L., Ibišević, M., Horozić, E., Arsenijević, J.,& Maksimović, Z.. (2022). Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts. in International Research Journal of Pure and Applied Chemistry
International Research Journal of Pure and Applied Chemistry., 23(5), 23-32.
https://doi.org/10.9734/irjpac/2022/v23i5788

Kozarević Cilović E, Dautović E, Halilčević D, Softić A, Srabović N, Šarić-Kundalić B, Delić N, Kolarević L, Mekić L, Ibišević M, Horozić E, Arsenijević J, Maksimović Z. Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts. in International Research Journal of Pure and Applied Chemistry. 2022;23(5):23-32.
doi:10.9734/irjpac/2022/v23i5788 .

Kozarević Cilović, Emina, Dautović, Esmeralda, Halilčević, Dalila, Softić, Adaleta, Srabović, Nahida, Šarić-Kundalić, Broza, Delić, Nina, Kolarević, Lamija, Mekić, Lejla, Ibišević, Merima, Horozić, Emir, Arsenijević, Jelena, Maksimović, Zoran, "Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts" in International Research Journal of Pure and Applied Chemistry, 23, no. 5 (2022):23-32,
https://doi.org/10.9734/irjpac/2022/v23i5788 . .

TY  - JOUR
AU  - Čilović-Kozarević, Ermina
AU  - Šarić-Kundalić, Broza
AU  - Ibišević, Merima
AU  - Horozić, Emir
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Arsenijević, Jelena
AU  - Maksimović, Zoran
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5107
AB  - Telekia speciosa (Schreb.) Baumg., Asteraceae, is widespread in Eastern and Central Europe and the Balkan Peninsula. Previous phytochemical investigations have revealed T. speciosa as a rich source of sesquiterpene lactone -isoalantolactone, especially in its underground parts. The aim of the present study was to analyze the essential oils from aerial and underground parts of T. speciosa and investigate their antimicrobial activity. Chemical composition of essential oils was determined by GC-FID/MS method leading to the identification of 67 compounds in total, with 15.77 % oxygenated monoterpenes, 7.77 % sesquiterpene hydrocarbons, 49.14 % oxygenated sesquiterpenes, and 12.37 % other compounds from aerial parts, and 3.80 % oxygenated monoterpenes, 3.13 % sesquiterpene hydrocarbons, 90.33 % oxygenated sesquiterpenes from underground parts essential oil. The main components from aerial parts were (E)-nerolidol (11.54 %) and caryophyllene oxide (10.54 %), while isoalantolactone was the predominant component from essential oil underground parts (83.41 %). The minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration of the essential oils were evaluated against six strains of bacteria and two strains of fungus using in vitro microdilution method. Both oils presented antimicrobial properties against pathogens Staphylococcus aureus, Bacilus cereus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. Inhibition of growth of tested microorganisms by T. speciosa underground parts essential oil was achieved with MICs ranging from 1.0 to 11.0 mg mL -1 , while MICs of aerial parts essential oil varied from 4.0 to 30.0 mg mL -1 . The obtained results contribute to the knowledge of antimicrobial properties of T. speciosa, which support traditional uses underground parts of the plant.
PB  - Institut za proučavanje lekovitog bilja "Dr. Josif Pančić", Beograd
T2  - Lekovite sirovine
T1  - GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg
VL  - 41
SP  - 35
EP  - 40
DO  - 10.5937/leksir2141035C
ER  - 

@article{
author = "Čilović-Kozarević, Ermina and Šarić-Kundalić, Broza and Ibišević, Merima and Horozić, Emir and Glamočlija, Jasmina and Soković, Marina and Arsenijević, Jelena and Maksimović, Zoran",
year = "2021",
abstract = "Telekia speciosa (Schreb.) Baumg., Asteraceae, is widespread in Eastern and Central Europe and the Balkan Peninsula. Previous phytochemical investigations have revealed T. speciosa as a rich source of sesquiterpene lactone -isoalantolactone, especially in its underground parts. The aim of the present study was to analyze the essential oils from aerial and underground parts of T. speciosa and investigate their antimicrobial activity. Chemical composition of essential oils was determined by GC-FID/MS method leading to the identification of 67 compounds in total, with 15.77 % oxygenated monoterpenes, 7.77 % sesquiterpene hydrocarbons, 49.14 % oxygenated sesquiterpenes, and 12.37 % other compounds from aerial parts, and 3.80 % oxygenated monoterpenes, 3.13 % sesquiterpene hydrocarbons, 90.33 % oxygenated sesquiterpenes from underground parts essential oil. The main components from aerial parts were (E)-nerolidol (11.54 %) and caryophyllene oxide (10.54 %), while isoalantolactone was the predominant component from essential oil underground parts (83.41 %). The minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration of the essential oils were evaluated against six strains of bacteria and two strains of fungus using in vitro microdilution method. Both oils presented antimicrobial properties against pathogens Staphylococcus aureus, Bacilus cereus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. Inhibition of growth of tested microorganisms by T. speciosa underground parts essential oil was achieved with MICs ranging from 1.0 to 11.0 mg mL -1 , while MICs of aerial parts essential oil varied from 4.0 to 30.0 mg mL -1 . The obtained results contribute to the knowledge of antimicrobial properties of T. speciosa, which support traditional uses underground parts of the plant.",
publisher = "Institut za proučavanje lekovitog bilja "Dr. Josif Pančić", Beograd",
journal = "Lekovite sirovine",
title = "GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg",
volume = "41",
pages = "35-40",
doi = "10.5937/leksir2141035C"
}

Čilović-Kozarević, E., Šarić-Kundalić, B., Ibišević, M., Horozić, E., Glamočlija, J., Soković, M., Arsenijević, J.,& Maksimović, Z.. (2021). GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg. in Lekovite sirovine
Institut za proučavanje lekovitog bilja "Dr. Josif Pančić", Beograd., 41, 35-40.
https://doi.org/10.5937/leksir2141035C

Čilović-Kozarević E, Šarić-Kundalić B, Ibišević M, Horozić E, Glamočlija J, Soković M, Arsenijević J, Maksimović Z. GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg. in Lekovite sirovine. 2021;41:35-40.
doi:10.5937/leksir2141035C .

Čilović-Kozarević, Ermina, Šarić-Kundalić, Broza, Ibišević, Merima, Horozić, Emir, Glamočlija, Jasmina, Soković, Marina, Arsenijević, Jelena, Maksimović, Zoran, "GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg" in Lekovite sirovine, 41 (2021):35-40,
https://doi.org/10.5937/leksir2141035C . .