TY  - JOUR
AU  - Ivanović, D
AU  - Medenica, Mirjana
AU  - Malenović, Anđelija
AU  - Jančić, Biljana
AU  - Misljenović, D
PY  - 2003
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/460
AB  - The optimization of RP-HPLC method defined the simultaneous influence of some important conditions, such as the mobile phase composition, pH of the mobile phase and temperature, on the separation and determination. The RP-HPLC method was done for the determination of paracetamol, caffeine and propyphenazone in a multicomponent pharmaceutical dosage form. The separation factor values define the optimal conditions, which were confirmed by analysing the appropriate mathematical models. The chromatographic system Hewlett Packard 1100 consisted of a HP 1100 pump, HP 1100 UV-VIS detector and HP integrator. Separations were performed on a Beckman Ultrasphere ODS 4.6 x 150 mm, 5 microns particle size column. Samples were introduced through a Rheodyne injector valve with a 20 microL sample loop. UV detection was performed at 265 nm and phenobarottons was used as an internal standard. The optimization was performed within the pH range from 2.5 to 6.0; temperature range from 20 degrees C to 55 degrees C and composition of the mobile phase methanol-water from (30:70 V/V) to (65:35 V/V). The three-D graphs, constructed with sixty-four experimental points, confirmed the optimal conditions for the determination of the investigated analgetic drugs.
T2  - Bollettino chimico farmaceutico
T1  - Optimization of the RP-HPLC method for multicomponent analgetic drug determination.
VL  - 142
IS  - 9
SP  - 386
EP  - 389
UR  - https://hdl.handle.net/21.15107/rcub_farfar_460
ER  - 

@article{
author = "Ivanović, D and Medenica, Mirjana and Malenović, Anđelija and Jančić, Biljana and Misljenović, D",
year = "2003",
abstract = "The optimization of RP-HPLC method defined the simultaneous influence of some important conditions, such as the mobile phase composition, pH of the mobile phase and temperature, on the separation and determination. The RP-HPLC method was done for the determination of paracetamol, caffeine and propyphenazone in a multicomponent pharmaceutical dosage form. The separation factor values define the optimal conditions, which were confirmed by analysing the appropriate mathematical models. The chromatographic system Hewlett Packard 1100 consisted of a HP 1100 pump, HP 1100 UV-VIS detector and HP integrator. Separations were performed on a Beckman Ultrasphere ODS 4.6 x 150 mm, 5 microns particle size column. Samples were introduced through a Rheodyne injector valve with a 20 microL sample loop. UV detection was performed at 265 nm and phenobarottons was used as an internal standard. The optimization was performed within the pH range from 2.5 to 6.0; temperature range from 20 degrees C to 55 degrees C and composition of the mobile phase methanol-water from (30:70 V/V) to (65:35 V/V). The three-D graphs, constructed with sixty-four experimental points, confirmed the optimal conditions for the determination of the investigated analgetic drugs.",
journal = "Bollettino chimico farmaceutico",
title = "Optimization of the RP-HPLC method for multicomponent analgetic drug determination.",
volume = "142",
number = "9",
pages = "386-389",
url = "https://hdl.handle.net/21.15107/rcub_farfar_460"
}

Ivanović, D., Medenica, M., Malenović, A., Jančić, B.,& Misljenović, D.. (2003). Optimization of the RP-HPLC method for multicomponent analgetic drug determination.. in Bollettino chimico farmaceutico, 142(9), 386-389.
https://hdl.handle.net/21.15107/rcub_farfar_460

Ivanović D, Medenica M, Malenović A, Jančić B, Misljenović D. Optimization of the RP-HPLC method for multicomponent analgetic drug determination.. in Bollettino chimico farmaceutico. 2003;142(9):386-389.
https://hdl.handle.net/21.15107/rcub_farfar_460 .

Ivanović, D, Medenica, Mirjana, Malenović, Anđelija, Jančić, Biljana, Misljenović, D, "Optimization of the RP-HPLC method for multicomponent analgetic drug determination." in Bollettino chimico farmaceutico, 142, no. 9 (2003):386-389,
https://hdl.handle.net/21.15107/rcub_farfar_460 .

TY  - JOUR
AU  - Medenica, Mirjana
AU  - Ivanović, D
AU  - Marković, S
AU  - Malenović, Anđelija
AU  - Misljenović, D
PY  - 2003
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/423
AB  - Optimization of important conditions for the reversed-phase high-performance liquid chromatographic method was done for the separation of the active ingredients in Marcaine(R) adrenaline injections (bupivacaine hydrochloride 2.5 mg and adrenaline 5.0 mug). Simultaneous influence of several conditions, such as the mobile phase composition, pH of the mobile phase, and temperature, on important chromatographic criteria for the separation, was investigated. The separation factor values defined the optimal conditions, which were confirmed by analysing the appropriate mathematical models. The 3-D graphs, constructed with sixty-four experimental points, were investigated, and the results showed that the optimal separation was achieved with the mobile phase of methanol-water (65: 35 v/v), by adjusting pH to 3.5 and the temperature range from 20degreesC to 30degreesC. The optimized method was validated and the obtained results were statistically evaluated.
PB  - Marcel Dekker Inc, New York
T2  - Journal of Liquid Chromatography & Related Technologies
T1  - Optimization of an RP-HPLC method for drug control analysis
VL  - 26
IS  - 20
SP  - 3401
EP  - 3412
DO  - 10.1081/JLC-120025598
ER  - 

@article{
author = "Medenica, Mirjana and Ivanović, D and Marković, S and Malenović, Anđelija and Misljenović, D",
year = "2003",
abstract = "Optimization of important conditions for the reversed-phase high-performance liquid chromatographic method was done for the separation of the active ingredients in Marcaine(R) adrenaline injections (bupivacaine hydrochloride 2.5 mg and adrenaline 5.0 mug). Simultaneous influence of several conditions, such as the mobile phase composition, pH of the mobile phase, and temperature, on important chromatographic criteria for the separation, was investigated. The separation factor values defined the optimal conditions, which were confirmed by analysing the appropriate mathematical models. The 3-D graphs, constructed with sixty-four experimental points, were investigated, and the results showed that the optimal separation was achieved with the mobile phase of methanol-water (65: 35 v/v), by adjusting pH to 3.5 and the temperature range from 20degreesC to 30degreesC. The optimized method was validated and the obtained results were statistically evaluated.",
publisher = "Marcel Dekker Inc, New York",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Optimization of an RP-HPLC method for drug control analysis",
volume = "26",
number = "20",
pages = "3401-3412",
doi = "10.1081/JLC-120025598"
}

Medenica, M., Ivanović, D., Marković, S., Malenović, A.,& Misljenović, D.. (2003). Optimization of an RP-HPLC method for drug control analysis. in Journal of Liquid Chromatography & Related Technologies
Marcel Dekker Inc, New York., 26(20), 3401-3412.
https://doi.org/10.1081/JLC-120025598

Medenica M, Ivanović D, Marković S, Malenović A, Misljenović D. Optimization of an RP-HPLC method for drug control analysis. in Journal of Liquid Chromatography & Related Technologies. 2003;26(20):3401-3412.
doi:10.1081/JLC-120025598 .

Medenica, Mirjana, Ivanović, D, Marković, S, Malenović, Anđelija, Misljenović, D, "Optimization of an RP-HPLC method for drug control analysis" in Journal of Liquid Chromatography & Related Technologies, 26, no. 20 (2003):3401-3412,
https://doi.org/10.1081/JLC-120025598 . .