TY  - JOUR
AU  - Pantelić, Ivana
AU  - Lukić, Milica
AU  - Gojgić-Cvijović, Gordana
AU  - Jakovljević, Dragica
AU  - Nikolić, Ines
AU  - Lunter, Dominique Jasmin
AU  - Daniels, Rolf
AU  - Savić, Snežana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3500
AB  - Ongoing demand in sustainable and biocompatible drug dosage forms is reflected in the search for novel pharmaceutical excipients with equal properties. A group of microbial exopolysaccharides offers a variety of biopolymers with many alleged uses and effects. This study aims to assess applicative properties of levan obtained from Bacillus licheniformis NS032, focusing on its potential co-stabilizing and drug release-controlling functions in pertaining emulsion systems. Despite its high molecular weight and partial existence in globular nanometric structures (180-190 nm), levan was successfully incorporated into both tested colloidal systems: those stabilized with synthetic/anionic or natural-origin/non-ionic emulsifiers. In the tested levan concentrations range (0.2-3.0% w/w) the monitored flow and thermal parameters failed to show linear concentration dependence, which prompted us to revisit certain colloidal fundamentals of this biopolymer. Being a part of the external phase of the investigated emulsion systems, levan contributed to formation of a matrix-like environment, offering additional stabilization of the microstructure and rheology modifying properties (hysteresis loop elevation as high as 4167±98 to 20792±3166 Pa•s−1), especially in case of the samples where lamellar liquid crystalline formation occurred. Apart from its good water solubility and considerable conformational flexibility, the investigated homofructan easily saturated the external phase of the samples stabilized with a conventional anionic emulsifier, leading to similar properties of 0.2% and 3.0% levan-containing samples. After closer consideration of thermal and release behavior, this was considered as a favorable property for a novel excipient, offering tailored formulation characteristics even with lower levan concentrations, consequently not compromising the potential cost of the final drug dosage form.
PB  - Elsevier
T2  - European Journal of Pharmaceutical Sciences
T1  - Bacillus licheniformis levan as a functional biopolymer in topical drug dosage forms: From basic colloidal considerations to actual pharmaceutical application
VL  - 142
DO  - 10.1016/j.ejps.2019.105109
ER  - 

@article{
author = "Pantelić, Ivana and Lukić, Milica and Gojgić-Cvijović, Gordana and Jakovljević, Dragica and Nikolić, Ines and Lunter, Dominique Jasmin and Daniels, Rolf and Savić, Snežana",
year = "2020",
abstract = "Ongoing demand in sustainable and biocompatible drug dosage forms is reflected in the search for novel pharmaceutical excipients with equal properties. A group of microbial exopolysaccharides offers a variety of biopolymers with many alleged uses and effects. This study aims to assess applicative properties of levan obtained from Bacillus licheniformis NS032, focusing on its potential co-stabilizing and drug release-controlling functions in pertaining emulsion systems. Despite its high molecular weight and partial existence in globular nanometric structures (180-190 nm), levan was successfully incorporated into both tested colloidal systems: those stabilized with synthetic/anionic or natural-origin/non-ionic emulsifiers. In the tested levan concentrations range (0.2-3.0% w/w) the monitored flow and thermal parameters failed to show linear concentration dependence, which prompted us to revisit certain colloidal fundamentals of this biopolymer. Being a part of the external phase of the investigated emulsion systems, levan contributed to formation of a matrix-like environment, offering additional stabilization of the microstructure and rheology modifying properties (hysteresis loop elevation as high as 4167±98 to 20792±3166 Pa•s−1), especially in case of the samples where lamellar liquid crystalline formation occurred. Apart from its good water solubility and considerable conformational flexibility, the investigated homofructan easily saturated the external phase of the samples stabilized with a conventional anionic emulsifier, leading to similar properties of 0.2% and 3.0% levan-containing samples. After closer consideration of thermal and release behavior, this was considered as a favorable property for a novel excipient, offering tailored formulation characteristics even with lower levan concentrations, consequently not compromising the potential cost of the final drug dosage form.",
publisher = "Elsevier",
journal = "European Journal of Pharmaceutical Sciences",
title = "Bacillus licheniformis levan as a functional biopolymer in topical drug dosage forms: From basic colloidal considerations to actual pharmaceutical application",
volume = "142",
doi = "10.1016/j.ejps.2019.105109"
}

Pantelić, I., Lukić, M., Gojgić-Cvijović, G., Jakovljević, D., Nikolić, I., Lunter, D. J., Daniels, R.,& Savić, S.. (2020). Bacillus licheniformis levan as a functional biopolymer in topical drug dosage forms: From basic colloidal considerations to actual pharmaceutical application. in European Journal of Pharmaceutical Sciences
Elsevier., 142.
https://doi.org/10.1016/j.ejps.2019.105109

Pantelić I, Lukić M, Gojgić-Cvijović G, Jakovljević D, Nikolić I, Lunter DJ, Daniels R, Savić S. Bacillus licheniformis levan as a functional biopolymer in topical drug dosage forms: From basic colloidal considerations to actual pharmaceutical application. in European Journal of Pharmaceutical Sciences. 2020;142.
doi:10.1016/j.ejps.2019.105109 .

Pantelić, Ivana, Lukić, Milica, Gojgić-Cvijović, Gordana, Jakovljević, Dragica, Nikolić, Ines, Lunter, Dominique Jasmin, Daniels, Rolf, Savić, Snežana, "Bacillus licheniformis levan as a functional biopolymer in topical drug dosage forms: From basic colloidal considerations to actual pharmaceutical application" in European Journal of Pharmaceutical Sciences, 142 (2020),
https://doi.org/10.1016/j.ejps.2019.105109 . .

TY  - JOUR
AU  - Nikolić, Ines
AU  - Lunter, Dominique
AU  - Ranđelović, Danijela
AU  - Žugić, Ana R.
AU  - Tadić, Vanja M.
AU  - Marković, Bojan
AU  - Cekić, Nebojša
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Potparević, Biljana
AU  - Daniels, Rolf
AU  - Savić, Snežana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3084
AB  - The objective of this work was to investigate and profoundly characterize low-energy nanoemulsions as multifunctional carriers, with slight reference to dermal administration. An evidence-based approach was offered for deepening the knowledge on their formation via spontaneous emulsification. Curcumin, a compound of natural origin, potentially powerful therapeutic, was chosen as a model API. Due to curcumin's demanding properties (instability, poor solubility, low permeability), its potentials remain unreached. Low-energy nanoemulsions were considered carriers capable of overcoming imposed obstacles. Formulation consisting of Polysorbate 80 and soybean lecithin as stabilizers (9:1, 10%), medium-chain triglycerides as the oil phase (10%) and ultrapure water was selected for curcumin incorporation in 3 different concentrations (1, 2 and 3 mg/mL). Physicochemical stability was demonstrated during 3 months of monitoring (mean droplet size: 111.3-146.8 nm; PDI  lt  0.2; pH: 4.73-5.73). Curcumin's release from developed vehicles followed Higuchi's kinetics. DPPH (IC50 = 0.1187 mg/ mL) and FRAP (1.19 +/- 0.02 mmol/g) assays confirmed that curcumin acts as a potent antioxidant through different mechanisms, with no alterations after incorporation in the formulation. High biocompatibility in line with antigenotoxic activity of curcumin-loaded formulations (protective and reparative) was estimated through Comet assay. A multidisciplinary approach is needed to fully characterize developed systems, directing them to more concrete application possibilities.
PB  - Elsevier Science BV, Amsterdam
T2  - International Journal of Pharmaceutics
T1  - Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application
VL  - 550
IS  - 1-2
SP  - 333
EP  - 346
DO  - 10.1016/j.ijpharm.2018.08.060
ER  - 

@article{
author = "Nikolić, Ines and Lunter, Dominique and Ranđelović, Danijela and Žugić, Ana R. and Tadić, Vanja M. and Marković, Bojan and Cekić, Nebojša and Živković, Lada and Topalović, Dijana and Potparević, Biljana and Daniels, Rolf and Savić, Snežana",
year = "2018",
abstract = "The objective of this work was to investigate and profoundly characterize low-energy nanoemulsions as multifunctional carriers, with slight reference to dermal administration. An evidence-based approach was offered for deepening the knowledge on their formation via spontaneous emulsification. Curcumin, a compound of natural origin, potentially powerful therapeutic, was chosen as a model API. Due to curcumin's demanding properties (instability, poor solubility, low permeability), its potentials remain unreached. Low-energy nanoemulsions were considered carriers capable of overcoming imposed obstacles. Formulation consisting of Polysorbate 80 and soybean lecithin as stabilizers (9:1, 10%), medium-chain triglycerides as the oil phase (10%) and ultrapure water was selected for curcumin incorporation in 3 different concentrations (1, 2 and 3 mg/mL). Physicochemical stability was demonstrated during 3 months of monitoring (mean droplet size: 111.3-146.8 nm; PDI  lt  0.2; pH: 4.73-5.73). Curcumin's release from developed vehicles followed Higuchi's kinetics. DPPH (IC50 = 0.1187 mg/ mL) and FRAP (1.19 +/- 0.02 mmol/g) assays confirmed that curcumin acts as a potent antioxidant through different mechanisms, with no alterations after incorporation in the formulation. High biocompatibility in line with antigenotoxic activity of curcumin-loaded formulations (protective and reparative) was estimated through Comet assay. A multidisciplinary approach is needed to fully characterize developed systems, directing them to more concrete application possibilities.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "International Journal of Pharmaceutics",
title = "Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application",
volume = "550",
number = "1-2",
pages = "333-346",
doi = "10.1016/j.ijpharm.2018.08.060"
}

Nikolić, I., Lunter, D., Ranđelović, D., Žugić, A. R., Tadić, V. M., Marković, B., Cekić, N., Živković, L., Topalović, D., Potparević, B., Daniels, R.,& Savić, S.. (2018). Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application. in International Journal of Pharmaceutics
Elsevier Science BV, Amsterdam., 550(1-2), 333-346.
https://doi.org/10.1016/j.ijpharm.2018.08.060

Nikolić I, Lunter D, Ranđelović D, Žugić AR, Tadić VM, Marković B, Cekić N, Živković L, Topalović D, Potparević B, Daniels R, Savić S. Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application. in International Journal of Pharmaceutics. 2018;550(1-2):333-346.
doi:10.1016/j.ijpharm.2018.08.060 .

Nikolić, Ines, Lunter, Dominique, Ranđelović, Danijela, Žugić, Ana R., Tadić, Vanja M., Marković, Bojan, Cekić, Nebojša, Živković, Lada, Topalović, Dijana, Potparević, Biljana, Daniels, Rolf, Savić, Snežana, "Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application" in International Journal of Pharmaceutics, 550, no. 1-2 (2018):333-346,
https://doi.org/10.1016/j.ijpharm.2018.08.060 . .

TY  - JOUR
AU  - Ilić, Tanja
AU  - Pantelić, Ivana
AU  - Lunter, Dominique
AU  - Dodević, Sanela
AU  - Marković, Bojan
AU  - Ranković, Dragana
AU  - Daniels, Rolf
AU  - Savić, Snežana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2826
AB  - This work aimed to prove the ability of "ready-to-use" topical vehicles based on alkyl polyglucoside-mixed emulsifier (with/without co-solvent modifications) to replace the conventionally used pharmacopoeial bases (e.g., non-ionic hydrophilic cream) in compounding practice. For this purpose, considering the regulatory efforts to establish alternative, scientifically valid methods for evaluating therapeutic equivalence of topical semisolids, we performed a comparative assessment of microstructure, selected critical quality attributes (CQAs) and in vitro/in vivo product performances, by utilizing aceclofenac as a model drug. The differences in composition between investigated samples have imposed remarkable variances in monitored CQAs (particularly in the amount of aceclofenac dissolved, rheological properties and water distribution mode), reflecting the distinct differences in microstructure formed, as partially observed by polarization microscopy and confocal Raman spectral imaging. Although not fully indicative of the in vivo performances, in vitro release data (vertical diffusion vs. immersion cells) proved the microstructure peculiarities, asserting the rheological properties as decisive factor for obtained liberation profiles. Contrary, in vitro permeation results obtained using pig ear epidermis correlated well with in vivo dermatopharmacokinetic data and distinguished unequivocally between tested formulations, emphasizing the importance of skin/vehicle interactions. In summary, suggested multi-faceted approach can provide adequate proof on topical semisolids therapeutic equivalence or lack thereof.
PB  - Elsevier Science BV, Amsterdam
T2  - International Journal of Pharmaceutics
T1  - Critical quality attributes, in vitro release and correlated in vitro skin permeation-in vivo tape stripping collective data for demonstrating therapeutic (non) equivalence of topical semisolids: A case study of "ready-to-use" vehicles
VL  - 528
IS  - 1-2
SP  - 253
EP  - 267
DO  - 10.1016/j.ijpharm.2017.06.018
ER  - 

@article{
author = "Ilić, Tanja and Pantelić, Ivana and Lunter, Dominique and Dodević, Sanela and Marković, Bojan and Ranković, Dragana and Daniels, Rolf and Savić, Snežana",
year = "2017",
abstract = "This work aimed to prove the ability of "ready-to-use" topical vehicles based on alkyl polyglucoside-mixed emulsifier (with/without co-solvent modifications) to replace the conventionally used pharmacopoeial bases (e.g., non-ionic hydrophilic cream) in compounding practice. For this purpose, considering the regulatory efforts to establish alternative, scientifically valid methods for evaluating therapeutic equivalence of topical semisolids, we performed a comparative assessment of microstructure, selected critical quality attributes (CQAs) and in vitro/in vivo product performances, by utilizing aceclofenac as a model drug. The differences in composition between investigated samples have imposed remarkable variances in monitored CQAs (particularly in the amount of aceclofenac dissolved, rheological properties and water distribution mode), reflecting the distinct differences in microstructure formed, as partially observed by polarization microscopy and confocal Raman spectral imaging. Although not fully indicative of the in vivo performances, in vitro release data (vertical diffusion vs. immersion cells) proved the microstructure peculiarities, asserting the rheological properties as decisive factor for obtained liberation profiles. Contrary, in vitro permeation results obtained using pig ear epidermis correlated well with in vivo dermatopharmacokinetic data and distinguished unequivocally between tested formulations, emphasizing the importance of skin/vehicle interactions. In summary, suggested multi-faceted approach can provide adequate proof on topical semisolids therapeutic equivalence or lack thereof.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "International Journal of Pharmaceutics",
title = "Critical quality attributes, in vitro release and correlated in vitro skin permeation-in vivo tape stripping collective data for demonstrating therapeutic (non) equivalence of topical semisolids: A case study of "ready-to-use" vehicles",
volume = "528",
number = "1-2",
pages = "253-267",
doi = "10.1016/j.ijpharm.2017.06.018"
}

Ilić, T., Pantelić, I., Lunter, D., Dodević, S., Marković, B., Ranković, D., Daniels, R.,& Savić, S.. (2017). Critical quality attributes, in vitro release and correlated in vitro skin permeation-in vivo tape stripping collective data for demonstrating therapeutic (non) equivalence of topical semisolids: A case study of "ready-to-use" vehicles. in International Journal of Pharmaceutics
Elsevier Science BV, Amsterdam., 528(1-2), 253-267.
https://doi.org/10.1016/j.ijpharm.2017.06.018

Ilić T, Pantelić I, Lunter D, Dodević S, Marković B, Ranković D, Daniels R, Savić S. Critical quality attributes, in vitro release and correlated in vitro skin permeation-in vivo tape stripping collective data for demonstrating therapeutic (non) equivalence of topical semisolids: A case study of "ready-to-use" vehicles. in International Journal of Pharmaceutics. 2017;528(1-2):253-267.
doi:10.1016/j.ijpharm.2017.06.018 .

Ilić, Tanja, Pantelić, Ivana, Lunter, Dominique, Dodević, Sanela, Marković, Bojan, Ranković, Dragana, Daniels, Rolf, Savić, Snežana, "Critical quality attributes, in vitro release and correlated in vitro skin permeation-in vivo tape stripping collective data for demonstrating therapeutic (non) equivalence of topical semisolids: A case study of "ready-to-use" vehicles" in International Journal of Pharmaceutics, 528, no. 1-2 (2017):253-267,
https://doi.org/10.1016/j.ijpharm.2017.06.018 . .

TY  - JOUR
AU  - Heck, Rouven
AU  - Lukić, Milica
AU  - Savić, Snežana
AU  - Daniels, Rolf
AU  - Lunter, Dominique
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2806
AB  - Aim: The purpose of this study was to evaluate skin permeation and penetration of nonivamide which has been formulated in novel film-forming formulations (FFFs). These formulations aim to prolong the availability of capsaicinoids which are used in long-term treatment of chronic pruritus. Methods: An oily solution of nonivamide was loaded into porous silica particles which then were suspended in an aqueous dispersion of a sustained release polymer. Permeation and penetration experiments were performed ex vivo with postauricular porcine skin using modified Franz diffusion cells. The penetrated drug amount was assessed ex vivo by skin surface biopsy followed by cryo-sectioning. Furthermore, in vivo skin irritation experiments were performed to compare the potential skin irritation caused by the FFFs to conventionally used semi-solid formulations. Results: Permeation rates of nonivamide from FFF through the skin are comparable to that from clinically used immediate release formulations. This elucidates the therapeutic safety profile of the novel FFF. Penetration studies confirmed the prolonged drug availability at the site of action. FFFs were found not to irritate the skin of healthy volunteers. Conclusion: FFFs with sustained nonivamide penetration represent safe and easy-to-use formulations. They therefore may improve the treatment of chronic pruritus with capsaicinoids by enhancing patient compliance through a sustained release regime.
PB  - Elsevier Science BV, Amsterdam
T2  - European Journal of Pharmaceutical Sciences
T1  - Ex vivo skin permeation and penetration of nonivamide from and in vivo skin tolerability of film-forming formulations containing porous silica
VL  - 106
SP  - 34
EP  - 40
DO  - 10.1016/j.ejps.2017.05.045
ER  - 

@article{
author = "Heck, Rouven and Lukić, Milica and Savić, Snežana and Daniels, Rolf and Lunter, Dominique",
year = "2017",
abstract = "Aim: The purpose of this study was to evaluate skin permeation and penetration of nonivamide which has been formulated in novel film-forming formulations (FFFs). These formulations aim to prolong the availability of capsaicinoids which are used in long-term treatment of chronic pruritus. Methods: An oily solution of nonivamide was loaded into porous silica particles which then were suspended in an aqueous dispersion of a sustained release polymer. Permeation and penetration experiments were performed ex vivo with postauricular porcine skin using modified Franz diffusion cells. The penetrated drug amount was assessed ex vivo by skin surface biopsy followed by cryo-sectioning. Furthermore, in vivo skin irritation experiments were performed to compare the potential skin irritation caused by the FFFs to conventionally used semi-solid formulations. Results: Permeation rates of nonivamide from FFF through the skin are comparable to that from clinically used immediate release formulations. This elucidates the therapeutic safety profile of the novel FFF. Penetration studies confirmed the prolonged drug availability at the site of action. FFFs were found not to irritate the skin of healthy volunteers. Conclusion: FFFs with sustained nonivamide penetration represent safe and easy-to-use formulations. They therefore may improve the treatment of chronic pruritus with capsaicinoids by enhancing patient compliance through a sustained release regime.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "European Journal of Pharmaceutical Sciences",
title = "Ex vivo skin permeation and penetration of nonivamide from and in vivo skin tolerability of film-forming formulations containing porous silica",
volume = "106",
pages = "34-40",
doi = "10.1016/j.ejps.2017.05.045"
}

Heck, R., Lukić, M., Savić, S., Daniels, R.,& Lunter, D.. (2017). Ex vivo skin permeation and penetration of nonivamide from and in vivo skin tolerability of film-forming formulations containing porous silica. in European Journal of Pharmaceutical Sciences
Elsevier Science BV, Amsterdam., 106, 34-40.
https://doi.org/10.1016/j.ejps.2017.05.045

Heck R, Lukić M, Savić S, Daniels R, Lunter D. Ex vivo skin permeation and penetration of nonivamide from and in vivo skin tolerability of film-forming formulations containing porous silica. in European Journal of Pharmaceutical Sciences. 2017;106:34-40.
doi:10.1016/j.ejps.2017.05.045 .

Heck, Rouven, Lukić, Milica, Savić, Snežana, Daniels, Rolf, Lunter, Dominique, "Ex vivo skin permeation and penetration of nonivamide from and in vivo skin tolerability of film-forming formulations containing porous silica" in European Journal of Pharmaceutical Sciences, 106 (2017):34-40,
https://doi.org/10.1016/j.ejps.2017.05.045 . .

TY  - JOUR
AU  - Isailović, Tanja
AU  - Đorđević, Sanela
AU  - Marković, Bojan
AU  - Randelović, Danijela
AU  - Cekić, Nebojša
AU  - Lukić, Milica
AU  - Pantelić, Ivana
AU  - Daniels, Rolf
AU  - Savić, Snežana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2716
AB  - We aimed to develop lecithin-based nanoemulsions intended for effective aceclofenac (ACF) skin delivery utilizing sucrose esters [sucrose palmitate (SP) and sucrose stearate (SS)] as additional stabilizers and penetration enhancers. To find the suitable surfactant mixtures and levels of process variables (homogenization pressure and number of cycles-high pressure homogenization manufacturing method) that result in drug-loaded nanoemulsions with minimal droplet size and narrow size distribution, a combined mixture-process experimental design was employed. Based on optimization data, selected nanoemulsions were evaluated regarding morphology, surface charge, drug-excipient interactions, physical stability, and in vivo skin performances (skin penetration and irritation potential). The predicted physicochemical properties and storage stability were proved satisfying for ACF-loaded nanoemulsions containing 2% of SP in the blend with 0%-1% of SS and 1%-2% of egg lecithin (produced at 50 degrees C/20 cycles/800 bar). Additionally, the in vivo tape stripping demonstrated superior ACF skin absorption from these nanoemulsions, particularly from those containing 2% of SP, 0.5% of SS, and 1.5% of egg lecithin, when comparing with the sample costabilized by conventional surfactant-polysorbate 80. In summary, the combined mixture-process experimental design was shown as a feasible tool for formulation development of multisurfactant-based nanosized delivery systems with potentially improved overall product performances.
PB  - Elsevier Science Inc, New York
T2  - Journal of Pharmaceutical Sciences
T1  - Biocompatible Nanoemulsions for Improved Aceclofenac Skin Delivery: Formulation Approach Using Combined Mixture-Process Experimental Design
VL  - 105
IS  - 1
SP  - 308
EP  - 323
DO  - 10.1002/jps.24706
ER  - 

@article{
author = "Isailović, Tanja and Đorđević, Sanela and Marković, Bojan and Randelović, Danijela and Cekić, Nebojša and Lukić, Milica and Pantelić, Ivana and Daniels, Rolf and Savić, Snežana",
year = "2016",
abstract = "We aimed to develop lecithin-based nanoemulsions intended for effective aceclofenac (ACF) skin delivery utilizing sucrose esters [sucrose palmitate (SP) and sucrose stearate (SS)] as additional stabilizers and penetration enhancers. To find the suitable surfactant mixtures and levels of process variables (homogenization pressure and number of cycles-high pressure homogenization manufacturing method) that result in drug-loaded nanoemulsions with minimal droplet size and narrow size distribution, a combined mixture-process experimental design was employed. Based on optimization data, selected nanoemulsions were evaluated regarding morphology, surface charge, drug-excipient interactions, physical stability, and in vivo skin performances (skin penetration and irritation potential). The predicted physicochemical properties and storage stability were proved satisfying for ACF-loaded nanoemulsions containing 2% of SP in the blend with 0%-1% of SS and 1%-2% of egg lecithin (produced at 50 degrees C/20 cycles/800 bar). Additionally, the in vivo tape stripping demonstrated superior ACF skin absorption from these nanoemulsions, particularly from those containing 2% of SP, 0.5% of SS, and 1.5% of egg lecithin, when comparing with the sample costabilized by conventional surfactant-polysorbate 80. In summary, the combined mixture-process experimental design was shown as a feasible tool for formulation development of multisurfactant-based nanosized delivery systems with potentially improved overall product performances.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Pharmaceutical Sciences",
title = "Biocompatible Nanoemulsions for Improved Aceclofenac Skin Delivery: Formulation Approach Using Combined Mixture-Process Experimental Design",
volume = "105",
number = "1",
pages = "308-323",
doi = "10.1002/jps.24706"
}

Isailović, T., Đorđević, S., Marković, B., Randelović, D., Cekić, N., Lukić, M., Pantelić, I., Daniels, R.,& Savić, S.. (2016). Biocompatible Nanoemulsions for Improved Aceclofenac Skin Delivery: Formulation Approach Using Combined Mixture-Process Experimental Design. in Journal of Pharmaceutical Sciences
Elsevier Science Inc, New York., 105(1), 308-323.
https://doi.org/10.1002/jps.24706

Isailović T, Đorđević S, Marković B, Randelović D, Cekić N, Lukić M, Pantelić I, Daniels R, Savić S. Biocompatible Nanoemulsions for Improved Aceclofenac Skin Delivery: Formulation Approach Using Combined Mixture-Process Experimental Design. in Journal of Pharmaceutical Sciences. 2016;105(1):308-323.
doi:10.1002/jps.24706 .

Isailović, Tanja, Đorđević, Sanela, Marković, Bojan, Randelović, Danijela, Cekić, Nebojša, Lukić, Milica, Pantelić, Ivana, Daniels, Rolf, Savić, Snežana, "Biocompatible Nanoemulsions for Improved Aceclofenac Skin Delivery: Formulation Approach Using Combined Mixture-Process Experimental Design" in Journal of Pharmaceutical Sciences, 105, no. 1 (2016):308-323,
https://doi.org/10.1002/jps.24706 . .

TY  - JOUR
AU  - Žugić, Ana R.
AU  - Lunter, Dominique
AU  - Daniels, Rolf
AU  - Pantelić, Ivana
AU  - Tasić-Kostov, Marija
AU  - Tadić, Vanja M.
AU  - Mišić, Dušan
AU  - Arsić, Ivana A.
AU  - Savić, Snežana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2588
AB  - Topical treatment of skin infections is often limited by drawbacks related to both antimicrobial agents and their vehicles. In addition, considering the growing promotion of natural therapeutic products, our objective was to develop and evaluate naturally-based emulsion system, as prospective topical formulation for skin infections-treatment. Therefore, alkyl polyglucoside surfactants were used for stabilization of a vehicle serving as potential carrier for supercritical CO2-extract of Usnea barbata, lichen with well-documented antimicrobial activity, incorporated using two protocols and three concentrations. Comprehensive physicochemical characterization suggested possible involvement of extract's particles in stabilization of the investigated system. Raman spectral imaging served as the key method in disclosing extract's particles potential to participate in the microstructure of the tested emulsion system via three mechanisms: (1) particle-particle aggregation, (2) adsorption at the oil-water interface and (3) hydrophobic particle-surfactant interactions. Stated extract-vehicle interaction proved to be correlated to the preparation procedure and extract concentration on one hand and to affect the physicochemical and biopharmaceutical features of investigated system, on the other hand. Thereafter, formulation with the best preliminary stability and liberation profile was selected for further efficiency and in vivo skin irritation potential evaluation, implying pertinent in vitro antimicrobial activity against G+ bacteria and overall satisfying preliminary safety profile.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Pharmaceutical Development and Technology
T1  - Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product
VL  - 21
IS  - 5
SP  - 563
EP  - 575
DO  - 10.3109/10837450.2015.1026606
ER  - 

@article{
author = "Žugić, Ana R. and Lunter, Dominique and Daniels, Rolf and Pantelić, Ivana and Tasić-Kostov, Marija and Tadić, Vanja M. and Mišić, Dušan and Arsić, Ivana A. and Savić, Snežana",
year = "2016",
abstract = "Topical treatment of skin infections is often limited by drawbacks related to both antimicrobial agents and their vehicles. In addition, considering the growing promotion of natural therapeutic products, our objective was to develop and evaluate naturally-based emulsion system, as prospective topical formulation for skin infections-treatment. Therefore, alkyl polyglucoside surfactants were used for stabilization of a vehicle serving as potential carrier for supercritical CO2-extract of Usnea barbata, lichen with well-documented antimicrobial activity, incorporated using two protocols and three concentrations. Comprehensive physicochemical characterization suggested possible involvement of extract's particles in stabilization of the investigated system. Raman spectral imaging served as the key method in disclosing extract's particles potential to participate in the microstructure of the tested emulsion system via three mechanisms: (1) particle-particle aggregation, (2) adsorption at the oil-water interface and (3) hydrophobic particle-surfactant interactions. Stated extract-vehicle interaction proved to be correlated to the preparation procedure and extract concentration on one hand and to affect the physicochemical and biopharmaceutical features of investigated system, on the other hand. Thereafter, formulation with the best preliminary stability and liberation profile was selected for further efficiency and in vivo skin irritation potential evaluation, implying pertinent in vitro antimicrobial activity against G+ bacteria and overall satisfying preliminary safety profile.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Pharmaceutical Development and Technology",
title = "Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product",
volume = "21",
number = "5",
pages = "563-575",
doi = "10.3109/10837450.2015.1026606"
}

Žugić, A. R., Lunter, D., Daniels, R., Pantelić, I., Tasić-Kostov, M., Tadić, V. M., Mišić, D., Arsić, I. A.,& Savić, S.. (2016). Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product. in Pharmaceutical Development and Technology
Taylor & Francis Ltd, Abingdon., 21(5), 563-575.
https://doi.org/10.3109/10837450.2015.1026606

Žugić AR, Lunter D, Daniels R, Pantelić I, Tasić-Kostov M, Tadić VM, Mišić D, Arsić IA, Savić S. Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product. in Pharmaceutical Development and Technology. 2016;21(5):563-575.
doi:10.3109/10837450.2015.1026606 .

Žugić, Ana R., Lunter, Dominique, Daniels, Rolf, Pantelić, Ivana, Tasić-Kostov, Marija, Tadić, Vanja M., Mišić, Dušan, Arsić, Ivana A., Savić, Snežana, "Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product" in Pharmaceutical Development and Technology, 21, no. 5 (2016):563-575,
https://doi.org/10.3109/10837450.2015.1026606 . .

TY  - JOUR
AU  - Đorđević, Sanela
AU  - Cekić, Nebojša
AU  - Savić, Miroslav
AU  - Isailović, Tanja
AU  - Ranđelović, Danijela
AU  - Marković, Bojan
AU  - Savić, Saša R.
AU  - Timić-Stamenić, Tamara
AU  - Daniels, Rolf
AU  - Savić, Snežana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2346
AB  - This paper describes design and evaluation of parenteral lecithin-based nanoemulsions intended for brain delivery of risperidone, a poorly water-soluble psychopharmacological drug. The nanoemulsions were prepared through cold/hot high pressure homogenization and characterized regarding droplet size, polydispersity, surface charge, morphology, drug-vehicle interactions, and physical stability. To estimate the simultaneous influence of nanoemulsion formulation and preparation parameters-co-emulsifier type, aqueous phase type, homogenization temperature-on the critical quality attributes of developed nanoemulsions, a general factorial experimental design was applied. From the established design space and stability data, promising risperidone-loaded nanoemulsions (mean size about 160 nm, size distribution  lt 0.15, zeta potential around -50 mV), containing sodium oleate in the aqueous phase and polysorbate 80, poloxamer 188 or Solutol (R) HS15 as co-emulsifier, were produced by hot homogenization and their ability to improve risperidone delivery to the brain was assessed in rats. Pharmacokinetic study demonstrated erratic brain profiles of risperidone following intraperitoneal administration in selected nanoemulsions, most probably due to their different droplet surface properties (different composition of the stabilizing layer). Namely, polysorbate 80-costabilized nanoemulsion showed increased (1.4-7.4-fold higher) risperidone brain availability compared to other nanoemulsions and drug solution, suggesting this nanoemulsion as a promising carrier worth exploring further for brain targeting.
PB  - Elsevier Science BV, Amsterdam
T2  - International Journal of Pharmaceutics
T1  - Parenteral nanoemulsions as promising carriers for brain delivery of risperidone: Design, characterization and in vivo pharmacokinetic evaluation
VL  - 493
IS  - 1-2
SP  - 40
EP  - 54
DO  - 10.1016/j.ijpharm.2015.07.007
ER  - 

@article{
author = "Đorđević, Sanela and Cekić, Nebojša and Savić, Miroslav and Isailović, Tanja and Ranđelović, Danijela and Marković, Bojan and Savić, Saša R. and Timić-Stamenić, Tamara and Daniels, Rolf and Savić, Snežana",
year = "2015",
abstract = "This paper describes design and evaluation of parenteral lecithin-based nanoemulsions intended for brain delivery of risperidone, a poorly water-soluble psychopharmacological drug. The nanoemulsions were prepared through cold/hot high pressure homogenization and characterized regarding droplet size, polydispersity, surface charge, morphology, drug-vehicle interactions, and physical stability. To estimate the simultaneous influence of nanoemulsion formulation and preparation parameters-co-emulsifier type, aqueous phase type, homogenization temperature-on the critical quality attributes of developed nanoemulsions, a general factorial experimental design was applied. From the established design space and stability data, promising risperidone-loaded nanoemulsions (mean size about 160 nm, size distribution  lt 0.15, zeta potential around -50 mV), containing sodium oleate in the aqueous phase and polysorbate 80, poloxamer 188 or Solutol (R) HS15 as co-emulsifier, were produced by hot homogenization and their ability to improve risperidone delivery to the brain was assessed in rats. Pharmacokinetic study demonstrated erratic brain profiles of risperidone following intraperitoneal administration in selected nanoemulsions, most probably due to their different droplet surface properties (different composition of the stabilizing layer). Namely, polysorbate 80-costabilized nanoemulsion showed increased (1.4-7.4-fold higher) risperidone brain availability compared to other nanoemulsions and drug solution, suggesting this nanoemulsion as a promising carrier worth exploring further for brain targeting.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "International Journal of Pharmaceutics",
title = "Parenteral nanoemulsions as promising carriers for brain delivery of risperidone: Design, characterization and in vivo pharmacokinetic evaluation",
volume = "493",
number = "1-2",
pages = "40-54",
doi = "10.1016/j.ijpharm.2015.07.007"
}

Đorđević, S., Cekić, N., Savić, M., Isailović, T., Ranđelović, D., Marković, B., Savić, S. R., Timić-Stamenić, T., Daniels, R.,& Savić, S.. (2015). Parenteral nanoemulsions as promising carriers for brain delivery of risperidone: Design, characterization and in vivo pharmacokinetic evaluation. in International Journal of Pharmaceutics
Elsevier Science BV, Amsterdam., 493(1-2), 40-54.
https://doi.org/10.1016/j.ijpharm.2015.07.007

Đorđević S, Cekić N, Savić M, Isailović T, Ranđelović D, Marković B, Savić SR, Timić-Stamenić T, Daniels R, Savić S. Parenteral nanoemulsions as promising carriers for brain delivery of risperidone: Design, characterization and in vivo pharmacokinetic evaluation. in International Journal of Pharmaceutics. 2015;493(1-2):40-54.
doi:10.1016/j.ijpharm.2015.07.007 .

Đorđević, Sanela, Cekić, Nebojša, Savić, Miroslav, Isailović, Tanja, Ranđelović, Danijela, Marković, Bojan, Savić, Saša R., Timić-Stamenić, Tamara, Daniels, Rolf, Savić, Snežana, "Parenteral nanoemulsions as promising carriers for brain delivery of risperidone: Design, characterization and in vivo pharmacokinetic evaluation" in International Journal of Pharmaceutics, 493, no. 1-2 (2015):40-54,
https://doi.org/10.1016/j.ijpharm.2015.07.007 . .

TY  - JOUR
AU  - Čalija, Bojan
AU  - Savić, Snežana
AU  - Krajišnik, Danina
AU  - Daniels, Rolf
AU  - Vučen, Sonja
AU  - Marković, Bojan
AU  - Milić, Jela
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2429
AB  - The objectives of this study were to prepare films from submicron chitosan/Eudragit((R)) L100-55 polyelectrolyte complexes (CH/EL PEC) and to assess the influence of CH molecular weight and CH/EL mass ratio on their structure and drug-release properties. The films were obtained by a simple, environmentally friendly, casting/solvent evaporation method and the verapamil hydrochloride (VH) was used as model drug. Submicron size, narrow size distribution, and acceptable stability of CH/EL PECs were confirmed by DLS and laser Doppler microelectrophoresis. SEM analysis revealed nonporous inner structure and flat surface of the films. Interactions between comprising polymers and formation of CH/EL PEC were established by DSC and FT-IR spectroscopy. In vitro swelling and drug release studies revealed the pH sensitivity of the films, with burst drug release in acidic conditions (pH 1.2) and sustained release in phosphate buffers pH 5.8, 6.8, and 7.4. The slowest VH release was achieved from the films prepared from equal amounts of EL and CH of higher molecular weight, confirming the significance of the CH/EL ratio and CH molecular weight on their ability to sustain drug release. The obtained results suggested that presented, simple, and eco-friendly preparation procedure can be used to obtain pH-sensitive CH/EL PEC films with a promising potential as drug carriers.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Applied Polymer Science
T1  - pH-sensitive polyelectrolyte films derived from submicron chitosan/Eudragit((R)) L 100-55 complexes: Physicochemical characterization and in vitro drug release
VL  - 132
IS  - 39
DO  - 10.1002/app.42583
ER  - 

@article{
author = "Čalija, Bojan and Savić, Snežana and Krajišnik, Danina and Daniels, Rolf and Vučen, Sonja and Marković, Bojan and Milić, Jela",
year = "2015",
abstract = "The objectives of this study were to prepare films from submicron chitosan/Eudragit((R)) L100-55 polyelectrolyte complexes (CH/EL PEC) and to assess the influence of CH molecular weight and CH/EL mass ratio on their structure and drug-release properties. The films were obtained by a simple, environmentally friendly, casting/solvent evaporation method and the verapamil hydrochloride (VH) was used as model drug. Submicron size, narrow size distribution, and acceptable stability of CH/EL PECs were confirmed by DLS and laser Doppler microelectrophoresis. SEM analysis revealed nonporous inner structure and flat surface of the films. Interactions between comprising polymers and formation of CH/EL PEC were established by DSC and FT-IR spectroscopy. In vitro swelling and drug release studies revealed the pH sensitivity of the films, with burst drug release in acidic conditions (pH 1.2) and sustained release in phosphate buffers pH 5.8, 6.8, and 7.4. The slowest VH release was achieved from the films prepared from equal amounts of EL and CH of higher molecular weight, confirming the significance of the CH/EL ratio and CH molecular weight on their ability to sustain drug release. The obtained results suggested that presented, simple, and eco-friendly preparation procedure can be used to obtain pH-sensitive CH/EL PEC films with a promising potential as drug carriers.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Applied Polymer Science",
title = "pH-sensitive polyelectrolyte films derived from submicron chitosan/Eudragit((R)) L 100-55 complexes: Physicochemical characterization and in vitro drug release",
volume = "132",
number = "39",
doi = "10.1002/app.42583"
}

Čalija, B., Savić, S., Krajišnik, D., Daniels, R., Vučen, S., Marković, B.,& Milić, J.. (2015). pH-sensitive polyelectrolyte films derived from submicron chitosan/Eudragit((R)) L 100-55 complexes: Physicochemical characterization and in vitro drug release. in Journal of Applied Polymer Science
Wiley-Blackwell, Hoboken., 132(39).
https://doi.org/10.1002/app.42583

Čalija B, Savić S, Krajišnik D, Daniels R, Vučen S, Marković B, Milić J. pH-sensitive polyelectrolyte films derived from submicron chitosan/Eudragit((R)) L 100-55 complexes: Physicochemical characterization and in vitro drug release. in Journal of Applied Polymer Science. 2015;132(39).
doi:10.1002/app.42583 .

Čalija, Bojan, Savić, Snežana, Krajišnik, Danina, Daniels, Rolf, Vučen, Sonja, Marković, Bojan, Milić, Jela, "pH-sensitive polyelectrolyte films derived from submicron chitosan/Eudragit((R)) L 100-55 complexes: Physicochemical characterization and in vitro drug release" in Journal of Applied Polymer Science, 132, no. 39 (2015),
https://doi.org/10.1002/app.42583 . .

TY  - JOUR
AU  - Bogdanović-Marković, Dragana
AU  - Tasić-Kostov, Marija
AU  - Lukić, Milica
AU  - Isailović, Tanja
AU  - Krstonosić, Veljko
AU  - Daniels, Rolf
AU  - Savić, Snežana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2130
AB  - Alkyl polyglucosides (APGs) are a perfect amphiphilic structure, with excellent surface activity and solubility feature. The aim of this study is to develop a simple system, with a relatively low emulsifier content, composed of materials mainly naturally based and with no additional fatty alcohol. Hydroxystearyl alcohol and Hydroxystearyl glucoside, prepared with Jojoba and Hazelnut oil, medium chain triglycerides with or without Xylitylglucoside and Anhydroxylitol and Xylitol, have been investigated by using microscopy, rheology, thermal analysis, pH and conductimetry. Cyclic stress and in vivo skin irritation tests were also conducted. The investigated natural APG emulsifier has a capacity to form simple and stable emulsions of desirable rheological profile with improved hydration potential and to renew damaged skin, thus it can be safely applied as stabilizer in cosmetic and prospective pharmaceutical cream-bases.
PB  - Carl Hanser Verlag, Munich
T2  - Tenside Surfactants Detergents
T1  - Physicochemical Characterization and in vivo Skin Performance of a Novel Alkyl Polyglucoside Emulsifier in Natural Cosmetic Cream-Bases
VL  - 51
IS  - 2
SP  - 133
EP  - 145
DO  - 10.3139/113.110294
ER  - 

@article{
author = "Bogdanović-Marković, Dragana and Tasić-Kostov, Marija and Lukić, Milica and Isailović, Tanja and Krstonosić, Veljko and Daniels, Rolf and Savić, Snežana",
year = "2014",
abstract = "Alkyl polyglucosides (APGs) are a perfect amphiphilic structure, with excellent surface activity and solubility feature. The aim of this study is to develop a simple system, with a relatively low emulsifier content, composed of materials mainly naturally based and with no additional fatty alcohol. Hydroxystearyl alcohol and Hydroxystearyl glucoside, prepared with Jojoba and Hazelnut oil, medium chain triglycerides with or without Xylitylglucoside and Anhydroxylitol and Xylitol, have been investigated by using microscopy, rheology, thermal analysis, pH and conductimetry. Cyclic stress and in vivo skin irritation tests were also conducted. The investigated natural APG emulsifier has a capacity to form simple and stable emulsions of desirable rheological profile with improved hydration potential and to renew damaged skin, thus it can be safely applied as stabilizer in cosmetic and prospective pharmaceutical cream-bases.",
publisher = "Carl Hanser Verlag, Munich",
journal = "Tenside Surfactants Detergents",
title = "Physicochemical Characterization and in vivo Skin Performance of a Novel Alkyl Polyglucoside Emulsifier in Natural Cosmetic Cream-Bases",
volume = "51",
number = "2",
pages = "133-145",
doi = "10.3139/113.110294"
}

Bogdanović-Marković, D., Tasić-Kostov, M., Lukić, M., Isailović, T., Krstonosić, V., Daniels, R.,& Savić, S.. (2014). Physicochemical Characterization and in vivo Skin Performance of a Novel Alkyl Polyglucoside Emulsifier in Natural Cosmetic Cream-Bases. in Tenside Surfactants Detergents
Carl Hanser Verlag, Munich., 51(2), 133-145.
https://doi.org/10.3139/113.110294

Bogdanović-Marković D, Tasić-Kostov M, Lukić M, Isailović T, Krstonosić V, Daniels R, Savić S. Physicochemical Characterization and in vivo Skin Performance of a Novel Alkyl Polyglucoside Emulsifier in Natural Cosmetic Cream-Bases. in Tenside Surfactants Detergents. 2014;51(2):133-145.
doi:10.3139/113.110294 .

Bogdanović-Marković, Dragana, Tasić-Kostov, Marija, Lukić, Milica, Isailović, Tanja, Krstonosić, Veljko, Daniels, Rolf, Savić, Snežana, "Physicochemical Characterization and in vivo Skin Performance of a Novel Alkyl Polyglucoside Emulsifier in Natural Cosmetic Cream-Bases" in Tenside Surfactants Detergents, 51, no. 2 (2014):133-145,
https://doi.org/10.3139/113.110294 . .

TY  - CHAP
AU  - Pantelić, Ivana
AU  - Lukić, Milica
AU  - Daniels, Rolf
AU  - Savić, Snežana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2246
AB  - Skin permeation and penetration assessment is important not only for determining efficacy of a topical product, but also when comparing different formulations during development. This chapter reviews methods for dermal availability assessment of delivery systems, with their advantages and shortcomings, and examples of their practical application with Alkyl Polyglucoside-based preparations. Alkyl Polyglucosides are used in many different delivery systems with various model actives. Systems stabilized with Alkyl Polyglucoside surfactants provide highly satisfactory cutaneous delivery compared with reference samples. This is mainly attributed to the characteristic APG-based colloidal structure and its ability to provide a combined enhancing effect with co-solvents. Microemulsions for dermal/transdermal delivery are also becoming popular due to their high solubilization potential. Alkyl Polyglucoside surfactants are also being considered for development of nanosystems.
PB  - Elsevier Inc.
T2  - Alkyl Polyglucosides: From Natural-Origin Surfactants to Prospective Delivery Systems
T1  - Alkyl Polyglucoside-based delivery systems: In vitro/in vivo skin absorption assessment
SP  - 107
EP  - 134
DO  - 10.1533/9781908818775.107
ER  - 

@inbook{
author = "Pantelić, Ivana and Lukić, Milica and Daniels, Rolf and Savić, Snežana",
year = "2014",
abstract = "Skin permeation and penetration assessment is important not only for determining efficacy of a topical product, but also when comparing different formulations during development. This chapter reviews methods for dermal availability assessment of delivery systems, with their advantages and shortcomings, and examples of their practical application with Alkyl Polyglucoside-based preparations. Alkyl Polyglucosides are used in many different delivery systems with various model actives. Systems stabilized with Alkyl Polyglucoside surfactants provide highly satisfactory cutaneous delivery compared with reference samples. This is mainly attributed to the characteristic APG-based colloidal structure and its ability to provide a combined enhancing effect with co-solvents. Microemulsions for dermal/transdermal delivery are also becoming popular due to their high solubilization potential. Alkyl Polyglucoside surfactants are also being considered for development of nanosystems.",
publisher = "Elsevier Inc.",
journal = "Alkyl Polyglucosides: From Natural-Origin Surfactants to Prospective Delivery Systems",
booktitle = "Alkyl Polyglucoside-based delivery systems: In vitro/in vivo skin absorption assessment",
pages = "107-134",
doi = "10.1533/9781908818775.107"
}

Pantelić, I., Lukić, M., Daniels, R.,& Savić, S.. (2014). Alkyl Polyglucoside-based delivery systems: In vitro/in vivo skin absorption assessment. in Alkyl Polyglucosides: From Natural-Origin Surfactants to Prospective Delivery Systems
Elsevier Inc.., 107-134.
https://doi.org/10.1533/9781908818775.107

Pantelić I, Lukić M, Daniels R, Savić S. Alkyl Polyglucoside-based delivery systems: In vitro/in vivo skin absorption assessment. in Alkyl Polyglucosides: From Natural-Origin Surfactants to Prospective Delivery Systems. 2014;:107-134.
doi:10.1533/9781908818775.107 .

Pantelić, Ivana, Lukić, Milica, Daniels, Rolf, Savić, Snežana, "Alkyl Polyglucoside-based delivery systems: In vitro/in vivo skin absorption assessment" in Alkyl Polyglucosides: From Natural-Origin Surfactants to Prospective Delivery Systems (2014):107-134,
https://doi.org/10.1533/9781908818775.107 . .

TY  - JOUR
AU  - Pantelić, Ivana
AU  - Lukić, Milica
AU  - Marković, Bojan
AU  - Lusiana
AU  - Hoffmann, Christine
AU  - Mueller-Goymann, Christel
AU  - Milić, Jela
AU  - Daniels, Rolf
AU  - Savić, Snežana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2194
AB  - Context: Approaching of pharmaceutical and cosmetic industries in some aspects inevitably influence formulation of topical pharmaceuticals, urging researchers to introduce novel excipients with proven benefits over traditional ones. In that context, alkyl polyglucosides (APG) emerge as prominent natural-origin emulsifiers with numerous favorable features (biodegradability, dermatological acceptability, desirable sensory properties). Objective: To evaluate APG-stabilized bases (alone and upon addition of isopropyl alcohol) and their impact on skin performance. A simultaneous in vitro/in vivo skin absorption study was conducted to evaluate whether the tape stripping technique could be recommended as an in vivo tool for skin penetration assessment during formulation optimization process. Materials and methods: After a comprehensive physicochemical characterization, biopharmaceutical properties of APG-bases versus reference ones were assessed through a combined in vitro (release/permeation) and in vivo approach. Results and discussion: Physicochemical characterization revealed substantial difference in structural ordering due to the formation of various mesomorphic phases. The enhancer-loaded APG base resulted in significantly higher drug levels at all depths into the stratum corneum, indicating that the selected enhancer along with specific colloidal structure has increased the extent of drug delivery. Conclusion: Results recommend the investigated emulsifier for stabilization of topical drug delivery systems, not only for their ability to sustain the addition of isopropyl alcohol which proved to be a valuable enhancer, but also satisfactory skin absorption and tolerability when compared to samples stabilized by conventional emulsifier. Tape stripping proved to be a useful and yet inexpensive tool for in vivo trials, able to discriminate subtle differences in dermal availability.
PB  - Informa Healthcare, London
T2  - Drug Development and Industrial Pharmacy
T1  - Development of a prospective isopropyl alcohol-loaded pharmaceutical base using simultaneous in vitro/in vivo characterization methods of skin performance
VL  - 40
IS  - 7
SP  - 960
EP  - 971
DO  - 10.3109/03639045.2013.794827
ER  - 

@article{
author = "Pantelić, Ivana and Lukić, Milica and Marković, Bojan and Lusiana and Hoffmann, Christine and Mueller-Goymann, Christel and Milić, Jela and Daniels, Rolf and Savić, Snežana",
year = "2014",
abstract = "Context: Approaching of pharmaceutical and cosmetic industries in some aspects inevitably influence formulation of topical pharmaceuticals, urging researchers to introduce novel excipients with proven benefits over traditional ones. In that context, alkyl polyglucosides (APG) emerge as prominent natural-origin emulsifiers with numerous favorable features (biodegradability, dermatological acceptability, desirable sensory properties). Objective: To evaluate APG-stabilized bases (alone and upon addition of isopropyl alcohol) and their impact on skin performance. A simultaneous in vitro/in vivo skin absorption study was conducted to evaluate whether the tape stripping technique could be recommended as an in vivo tool for skin penetration assessment during formulation optimization process. Materials and methods: After a comprehensive physicochemical characterization, biopharmaceutical properties of APG-bases versus reference ones were assessed through a combined in vitro (release/permeation) and in vivo approach. Results and discussion: Physicochemical characterization revealed substantial difference in structural ordering due to the formation of various mesomorphic phases. The enhancer-loaded APG base resulted in significantly higher drug levels at all depths into the stratum corneum, indicating that the selected enhancer along with specific colloidal structure has increased the extent of drug delivery. Conclusion: Results recommend the investigated emulsifier for stabilization of topical drug delivery systems, not only for their ability to sustain the addition of isopropyl alcohol which proved to be a valuable enhancer, but also satisfactory skin absorption and tolerability when compared to samples stabilized by conventional emulsifier. Tape stripping proved to be a useful and yet inexpensive tool for in vivo trials, able to discriminate subtle differences in dermal availability.",
publisher = "Informa Healthcare, London",
journal = "Drug Development and Industrial Pharmacy",
title = "Development of a prospective isopropyl alcohol-loaded pharmaceutical base using simultaneous in vitro/in vivo characterization methods of skin performance",
volume = "40",
number = "7",
pages = "960-971",
doi = "10.3109/03639045.2013.794827"
}

Pantelić, I., Lukić, M., Marković, B., Lusiana, Hoffmann, C., Mueller-Goymann, C., Milić, J., Daniels, R.,& Savić, S.. (2014). Development of a prospective isopropyl alcohol-loaded pharmaceutical base using simultaneous in vitro/in vivo characterization methods of skin performance. in Drug Development and Industrial Pharmacy
Informa Healthcare, London., 40(7), 960-971.
https://doi.org/10.3109/03639045.2013.794827

Pantelić I, Lukić M, Marković B, Lusiana, Hoffmann C, Mueller-Goymann C, Milić J, Daniels R, Savić S. Development of a prospective isopropyl alcohol-loaded pharmaceutical base using simultaneous in vitro/in vivo characterization methods of skin performance. in Drug Development and Industrial Pharmacy. 2014;40(7):960-971.
doi:10.3109/03639045.2013.794827 .

Pantelić, Ivana, Lukić, Milica, Marković, Bojan, Lusiana, Hoffmann, Christine, Mueller-Goymann, Christel, Milić, Jela, Daniels, Rolf, Savić, Snežana, "Development of a prospective isopropyl alcohol-loaded pharmaceutical base using simultaneous in vitro/in vivo characterization methods of skin performance" in Drug Development and Industrial Pharmacy, 40, no. 7 (2014):960-971,
https://doi.org/10.3109/03639045.2013.794827 . .

TY  - JOUR
AU  - Pantelić, Ivana
AU  - Lukić, Milica
AU  - Marković, Bojan
AU  - Daniels, Rolf
AU  - Vesić, Sonja
AU  - Vuleta, Gordana
AU  - Savić, Snežana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2177
AB  - Context: Alkyl polyglucoside surfactants (APG) remain prominent natural origin stabilizers offering a prospect of combining satisfactory stability with mild dermatological properties and complete biodegradability. Objective: With the purpose of adjusting the dose to a patient's needs, dilution of commercial corticosteroid formulations is a practice which may modify efficacy uncontrolledly. The rational of the study was to investigate whether a simple change in ready-to-use bases (co-solvent addition) could address these needs in a more predictive manner. Methods: Hydrocortisone (HC) delivery from such emulsion systems was comparatively assessed employing two in vivo methods: the established human skin blanching assay versus skin stripping technique. Results: HC permeation data obtained after three dose durations showed better overall performance of the APG-stabilized bases relative to reference ones. Although the solubility study showed that all the assessed active samples retained equal thermodynamic activity, diverse HC permeation/penetration implies the importance of the applied base's colloidal structure and/or changes endured. Isopropyl alcohol (IPA) addition offered faster drug penetration enhancement, while glycerol as a moisturizing agent influenced HC penetration through the increase in skin hydration. Conclusion: Although the performed in vivo methods cannot be considered alternative, skin stripping technique proved to be a cost-efficient mode of percutaneous penetration assessment, providing additional information on vehicle-skin interactions.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Pharmaceutical Development and Technology
T1  - Effect of small changes in natural origin-based emulsion systems on hydrocortisone skin absorption and performance: a comparison of two in vivo methods
VL  - 19
IS  - 1
SP  - 55
EP  - 64
DO  - 10.3109/10837450.2012.751407
ER  - 

@article{
author = "Pantelić, Ivana and Lukić, Milica and Marković, Bojan and Daniels, Rolf and Vesić, Sonja and Vuleta, Gordana and Savić, Snežana",
year = "2014",
abstract = "Context: Alkyl polyglucoside surfactants (APG) remain prominent natural origin stabilizers offering a prospect of combining satisfactory stability with mild dermatological properties and complete biodegradability. Objective: With the purpose of adjusting the dose to a patient's needs, dilution of commercial corticosteroid formulations is a practice which may modify efficacy uncontrolledly. The rational of the study was to investigate whether a simple change in ready-to-use bases (co-solvent addition) could address these needs in a more predictive manner. Methods: Hydrocortisone (HC) delivery from such emulsion systems was comparatively assessed employing two in vivo methods: the established human skin blanching assay versus skin stripping technique. Results: HC permeation data obtained after three dose durations showed better overall performance of the APG-stabilized bases relative to reference ones. Although the solubility study showed that all the assessed active samples retained equal thermodynamic activity, diverse HC permeation/penetration implies the importance of the applied base's colloidal structure and/or changes endured. Isopropyl alcohol (IPA) addition offered faster drug penetration enhancement, while glycerol as a moisturizing agent influenced HC penetration through the increase in skin hydration. Conclusion: Although the performed in vivo methods cannot be considered alternative, skin stripping technique proved to be a cost-efficient mode of percutaneous penetration assessment, providing additional information on vehicle-skin interactions.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Pharmaceutical Development and Technology",
title = "Effect of small changes in natural origin-based emulsion systems on hydrocortisone skin absorption and performance: a comparison of two in vivo methods",
volume = "19",
number = "1",
pages = "55-64",
doi = "10.3109/10837450.2012.751407"
}

Pantelić, I., Lukić, M., Marković, B., Daniels, R., Vesić, S., Vuleta, G.,& Savić, S.. (2014). Effect of small changes in natural origin-based emulsion systems on hydrocortisone skin absorption and performance: a comparison of two in vivo methods. in Pharmaceutical Development and Technology
Taylor & Francis Ltd, Abingdon., 19(1), 55-64.
https://doi.org/10.3109/10837450.2012.751407

Pantelić I, Lukić M, Marković B, Daniels R, Vesić S, Vuleta G, Savić S. Effect of small changes in natural origin-based emulsion systems on hydrocortisone skin absorption and performance: a comparison of two in vivo methods. in Pharmaceutical Development and Technology. 2014;19(1):55-64.
doi:10.3109/10837450.2012.751407 .

Pantelić, Ivana, Lukić, Milica, Marković, Bojan, Daniels, Rolf, Vesić, Sonja, Vuleta, Gordana, Savić, Snežana, "Effect of small changes in natural origin-based emulsion systems on hydrocortisone skin absorption and performance: a comparison of two in vivo methods" in Pharmaceutical Development and Technology, 19, no. 1 (2014):55-64,
https://doi.org/10.3109/10837450.2012.751407 . .

TY  - JOUR
AU  - Čalija, Bojan
AU  - Cekić, Nebojša
AU  - Savić, Snežana
AU  - Daniels, Rolf
AU  - Marković, Bojan
AU  - Milić, Jela
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1969
AB  - The primary objective of this study was to investigate the influence of the oligochitosan-Eudragit (R) L100-55 polyelectrolyte complex (OCH-EL PEC) on the pH-sensitivity of Eudragit (R) L100-55-treated alginate-oligochitosan microparticles. In order to achieve this, three types of naproxen-loaded microparticles were prepared under mild and environmentally friendly conditions using a custom made device with coaxial air flow: Ca-alginate (Ca-ALG), alginate-oligochitosan (ALG-OCH) and alginate-oligochitosan-Eudragit (R) L100-55 (ALG-OCH-EL) microparticles. After drying, the microparticles were subjected to microscopic analysis, and physicochemical and biopharmaceutical characterization. The non-covalent interaction between OCH and EL and the formation of OCH-EL PEC during the preparation procedure of the particles were verified by thermal and FT-IR analysis. The obtained particles exhibited acceptable sphericity and surface roughness due to the presence of the drug crystals (Ca-ALG particles) and OCH-EL PEC (ALG-OCH-EL particles). It was found that reinforcement of the ALG-OCH particles with OCH-EL PEC had no significant effect on the relatively high encapsulation efficiencies (>74.4%). The results of drug release studies confirmed the ability of ALG-OCH PEC to sustain drug release at pH 6.8 and 7.4. However, this PEC showed enhanced sensitivity to an acidic environment and to simulated intestinal fluid (pH 6.8) after prior exposure to an acidic medium. Additional treatment of ALG-OCH particles with EL and formation of "sandwich" ALG-OCH-EL PEC was essential not only to improve stability and decrease drug release in acidic medium, but also to achieve sustained release after the pH of dissolution medium was raised to 6.8. The obtained results suggested that ALG-OCH-EL microparticles have promising potential as pH-sensitive multiparticulate drug carriers for oral delivery of NSAIDs.
PB  - Elsevier Science BV, Amsterdam
T2  - Colloids and Surfaces B: Biointerfaces
T1  - pH-sensitive microparticles for oral drug delivery based on alginate/oligochitosan/Eudragit (R) L100-55 "sandwich" polyelectrolyte complex
VL  - 110
SP  - 395
EP  - 402
DO  - 10.1016/j.colsurfb.2013.05.016
ER  - 

@article{
author = "Čalija, Bojan and Cekić, Nebojša and Savić, Snežana and Daniels, Rolf and Marković, Bojan and Milić, Jela",
year = "2013",
abstract = "The primary objective of this study was to investigate the influence of the oligochitosan-Eudragit (R) L100-55 polyelectrolyte complex (OCH-EL PEC) on the pH-sensitivity of Eudragit (R) L100-55-treated alginate-oligochitosan microparticles. In order to achieve this, three types of naproxen-loaded microparticles were prepared under mild and environmentally friendly conditions using a custom made device with coaxial air flow: Ca-alginate (Ca-ALG), alginate-oligochitosan (ALG-OCH) and alginate-oligochitosan-Eudragit (R) L100-55 (ALG-OCH-EL) microparticles. After drying, the microparticles were subjected to microscopic analysis, and physicochemical and biopharmaceutical characterization. The non-covalent interaction between OCH and EL and the formation of OCH-EL PEC during the preparation procedure of the particles were verified by thermal and FT-IR analysis. The obtained particles exhibited acceptable sphericity and surface roughness due to the presence of the drug crystals (Ca-ALG particles) and OCH-EL PEC (ALG-OCH-EL particles). It was found that reinforcement of the ALG-OCH particles with OCH-EL PEC had no significant effect on the relatively high encapsulation efficiencies (>74.4%). The results of drug release studies confirmed the ability of ALG-OCH PEC to sustain drug release at pH 6.8 and 7.4. However, this PEC showed enhanced sensitivity to an acidic environment and to simulated intestinal fluid (pH 6.8) after prior exposure to an acidic medium. Additional treatment of ALG-OCH particles with EL and formation of "sandwich" ALG-OCH-EL PEC was essential not only to improve stability and decrease drug release in acidic medium, but also to achieve sustained release after the pH of dissolution medium was raised to 6.8. The obtained results suggested that ALG-OCH-EL microparticles have promising potential as pH-sensitive multiparticulate drug carriers for oral delivery of NSAIDs.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "pH-sensitive microparticles for oral drug delivery based on alginate/oligochitosan/Eudragit (R) L100-55 "sandwich" polyelectrolyte complex",
volume = "110",
pages = "395-402",
doi = "10.1016/j.colsurfb.2013.05.016"
}

Čalija, B., Cekić, N., Savić, S., Daniels, R., Marković, B.,& Milić, J.. (2013). pH-sensitive microparticles for oral drug delivery based on alginate/oligochitosan/Eudragit (R) L100-55 "sandwich" polyelectrolyte complex. in Colloids and Surfaces B: Biointerfaces
Elsevier Science BV, Amsterdam., 110, 395-402.
https://doi.org/10.1016/j.colsurfb.2013.05.016

Čalija B, Cekić N, Savić S, Daniels R, Marković B, Milić J. pH-sensitive microparticles for oral drug delivery based on alginate/oligochitosan/Eudragit (R) L100-55 "sandwich" polyelectrolyte complex. in Colloids and Surfaces B: Biointerfaces. 2013;110:395-402.
doi:10.1016/j.colsurfb.2013.05.016 .

Čalija, Bojan, Cekić, Nebojša, Savić, Snežana, Daniels, Rolf, Marković, Bojan, Milić, Jela, "pH-sensitive microparticles for oral drug delivery based on alginate/oligochitosan/Eudragit (R) L100-55 "sandwich" polyelectrolyte complex" in Colloids and Surfaces B: Biointerfaces, 110 (2013):395-402,
https://doi.org/10.1016/j.colsurfb.2013.05.016 . .

TY  - JOUR
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Cekić, Nebojša
AU  - Krajišnik, Danina
AU  - Daniels, Rolf
AU  - Savić, Snežana
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1941
AB  - Objectives: The aim of the presented work was to develop Ca-alginate microparticles for oral administration of naproxen reinforced with chitosan oligosaccharide (COS) with a special interest to examine the potential of COS for improvement of microparticles stability in simulated intestinal fluid (SIF). Method: Microparticles were prepared according to the two-step procedure using an air-jet device with varying calcium chloride and COS concentration in the gelling medium. All prepared microparticles were subjected to size determination, morphology, surface, and inner structure analysis by scanning electron microscopy (SEM), drug loading (DL) and encapsulation efficiency (EE), differential scanning calorimetry (DSC), Fourier transform infrared (FTIR) spectroscopy, in vitro swelling, and drug release studies. Results: In general, COS-treated microparticles were spherical in shape but somewhat deformed, exhibiting the surface roughness with the mean particle size less than 350 mu m. FT-IR and DSC studies confirmed the formation of polyelectrolyte complex (PEC) between alginate and COS, whereas chemical properties and crystalline state of naproxen were unaffected by the encapsulation process. Low naproxen solubility in the gelling medium and rapid entrapment resulted in high encapsulation efficiency (>80.0%). The results of swelling studies demonstrated that COS-treated particles were less sensitive to swelling and erosion in SIF in comparison to the nontreated particles. This resulted in prolonged drug release in SIF, which was dependent on the COS/alginate ratio. Conclusion: The obtained findings proved that COS could be used as an effective cross-linking agent for improvement of Ca-alginate microparticles stability in SIF, allowing prolonged release of the encapsulated drug after oral administration.
PB  - Informa Healthcare, London
T2  - Drug Development and Industrial Pharmacy
T1  - Chitosan oligosaccharide as prospective cross-linking agent for naproxen-loaded Ca-alginate microparticles with improved pH sensitivity
VL  - 39
IS  - 1
SP  - 77
EP  - 88
DO  - 10.3109/03639045.2012.658813
ER  - 

@article{
author = "Čalija, Bojan and Milić, Jela and Cekić, Nebojša and Krajišnik, Danina and Daniels, Rolf and Savić, Snežana",
year = "2013",
abstract = "Objectives: The aim of the presented work was to develop Ca-alginate microparticles for oral administration of naproxen reinforced with chitosan oligosaccharide (COS) with a special interest to examine the potential of COS for improvement of microparticles stability in simulated intestinal fluid (SIF). Method: Microparticles were prepared according to the two-step procedure using an air-jet device with varying calcium chloride and COS concentration in the gelling medium. All prepared microparticles were subjected to size determination, morphology, surface, and inner structure analysis by scanning electron microscopy (SEM), drug loading (DL) and encapsulation efficiency (EE), differential scanning calorimetry (DSC), Fourier transform infrared (FTIR) spectroscopy, in vitro swelling, and drug release studies. Results: In general, COS-treated microparticles were spherical in shape but somewhat deformed, exhibiting the surface roughness with the mean particle size less than 350 mu m. FT-IR and DSC studies confirmed the formation of polyelectrolyte complex (PEC) between alginate and COS, whereas chemical properties and crystalline state of naproxen were unaffected by the encapsulation process. Low naproxen solubility in the gelling medium and rapid entrapment resulted in high encapsulation efficiency (>80.0%). The results of swelling studies demonstrated that COS-treated particles were less sensitive to swelling and erosion in SIF in comparison to the nontreated particles. This resulted in prolonged drug release in SIF, which was dependent on the COS/alginate ratio. Conclusion: The obtained findings proved that COS could be used as an effective cross-linking agent for improvement of Ca-alginate microparticles stability in SIF, allowing prolonged release of the encapsulated drug after oral administration.",
publisher = "Informa Healthcare, London",
journal = "Drug Development and Industrial Pharmacy",
title = "Chitosan oligosaccharide as prospective cross-linking agent for naproxen-loaded Ca-alginate microparticles with improved pH sensitivity",
volume = "39",
number = "1",
pages = "77-88",
doi = "10.3109/03639045.2012.658813"
}

Čalija, B., Milić, J., Cekić, N., Krajišnik, D., Daniels, R.,& Savić, S.. (2013). Chitosan oligosaccharide as prospective cross-linking agent for naproxen-loaded Ca-alginate microparticles with improved pH sensitivity. in Drug Development and Industrial Pharmacy
Informa Healthcare, London., 39(1), 77-88.
https://doi.org/10.3109/03639045.2012.658813

Čalija B, Milić J, Cekić N, Krajišnik D, Daniels R, Savić S. Chitosan oligosaccharide as prospective cross-linking agent for naproxen-loaded Ca-alginate microparticles with improved pH sensitivity. in Drug Development and Industrial Pharmacy. 2013;39(1):77-88.
doi:10.3109/03639045.2012.658813 .

Čalija, Bojan, Milić, Jela, Cekić, Nebojša, Krajišnik, Danina, Daniels, Rolf, Savić, Snežana, "Chitosan oligosaccharide as prospective cross-linking agent for naproxen-loaded Ca-alginate microparticles with improved pH sensitivity" in Drug Development and Industrial Pharmacy, 39, no. 1 (2013):77-88,
https://doi.org/10.3109/03639045.2012.658813 . .

TY  - JOUR
AU  - Lukić, Milica
AU  - Pantelić, Ivana
AU  - Daniels, Rolf
AU  - Mueller-Goymann, Christel
AU  - Savić, Miroslav
AU  - Savić, Snežana
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1956
AB  - Mesomorphic behavior of the novel long-chain alkyl polyglucoside emulsifier comprising arachidyl alcohol (C-20), behenyl alcohol (C-22), and arachidyl glucoside was investigated in order to determine the prevalent stabilization mechanism and moisturizing capacity of emulsion systems based on it. For this to be accomplished thermoanalytical methods (differential scanning calorimetry and thermogravimetric analysis) coupled with microscopy, rheological, X-ray diffraction methods and a short-term in vivo study of skin hydration level were performed. Obtained results have proved that C-20/C-22 alkyl polyglucoside mixed emulsifier is able to provide the synergism between the two main types of lamellar phases, the liquid-crystalline (L alpha), and the gel crystalline (L beta) one, building the emulsion systems of different stability and performance. Formation of lamellar structures influenced for more than one half of water within the system to be entrapped. Conducted investigation of hydration potential in real-time conditions provided valuable information on the investigated emulsion vehicles' moisturizing potential as well as their contribution to the skin barrier improvement. Therefore, it could be expected that emulsions based on this alkyl polyglucoside emulsifier could influence the delivery of active ingredients of both the lipophilic and hydrophilic type. The employment of thermoanalytical methods in our work suggests the possibility for thermal methods to be used more frequently in the characterization of both the novel raw materials and the belonging emulsion systems.
PB  - Springer, Dordrecht
T2  - Journal of Thermal Analysis and Calorimetry
T1  - Moisturizing emulsion systems based on the novel long-chain alkyl polyglucoside emulsifier The contribution of thermoanalytical methods to the formulation development
VL  - 111
IS  - 3
SP  - 2045
EP  - 2057
DO  - 10.1007/s10973-012-2263-0
ER  - 

@article{
author = "Lukić, Milica and Pantelić, Ivana and Daniels, Rolf and Mueller-Goymann, Christel and Savić, Miroslav and Savić, Snežana",
year = "2013",
abstract = "Mesomorphic behavior of the novel long-chain alkyl polyglucoside emulsifier comprising arachidyl alcohol (C-20), behenyl alcohol (C-22), and arachidyl glucoside was investigated in order to determine the prevalent stabilization mechanism and moisturizing capacity of emulsion systems based on it. For this to be accomplished thermoanalytical methods (differential scanning calorimetry and thermogravimetric analysis) coupled with microscopy, rheological, X-ray diffraction methods and a short-term in vivo study of skin hydration level were performed. Obtained results have proved that C-20/C-22 alkyl polyglucoside mixed emulsifier is able to provide the synergism between the two main types of lamellar phases, the liquid-crystalline (L alpha), and the gel crystalline (L beta) one, building the emulsion systems of different stability and performance. Formation of lamellar structures influenced for more than one half of water within the system to be entrapped. Conducted investigation of hydration potential in real-time conditions provided valuable information on the investigated emulsion vehicles' moisturizing potential as well as their contribution to the skin barrier improvement. Therefore, it could be expected that emulsions based on this alkyl polyglucoside emulsifier could influence the delivery of active ingredients of both the lipophilic and hydrophilic type. The employment of thermoanalytical methods in our work suggests the possibility for thermal methods to be used more frequently in the characterization of both the novel raw materials and the belonging emulsion systems.",
publisher = "Springer, Dordrecht",
journal = "Journal of Thermal Analysis and Calorimetry",
title = "Moisturizing emulsion systems based on the novel long-chain alkyl polyglucoside emulsifier The contribution of thermoanalytical methods to the formulation development",
volume = "111",
number = "3",
pages = "2045-2057",
doi = "10.1007/s10973-012-2263-0"
}

Lukić, M., Pantelić, I., Daniels, R., Mueller-Goymann, C., Savić, M.,& Savić, S.. (2013). Moisturizing emulsion systems based on the novel long-chain alkyl polyglucoside emulsifier The contribution of thermoanalytical methods to the formulation development. in Journal of Thermal Analysis and Calorimetry
Springer, Dordrecht., 111(3), 2045-2057.
https://doi.org/10.1007/s10973-012-2263-0

Lukić M, Pantelić I, Daniels R, Mueller-Goymann C, Savić M, Savić S. Moisturizing emulsion systems based on the novel long-chain alkyl polyglucoside emulsifier The contribution of thermoanalytical methods to the formulation development. in Journal of Thermal Analysis and Calorimetry. 2013;111(3):2045-2057.
doi:10.1007/s10973-012-2263-0 .

Lukić, Milica, Pantelić, Ivana, Daniels, Rolf, Mueller-Goymann, Christel, Savić, Miroslav, Savić, Snežana, "Moisturizing emulsion systems based on the novel long-chain alkyl polyglucoside emulsifier The contribution of thermoanalytical methods to the formulation development" in Journal of Thermal Analysis and Calorimetry, 111, no. 3 (2013):2045-2057,
https://doi.org/10.1007/s10973-012-2263-0 . .

TY  - JOUR
AU  - Pantelić, Ivana
AU  - Lukić, Milica
AU  - Malenović, Anđelija
AU  - Reichl, Stephan
AU  - Hoffmann, Christine
AU  - Mueller-Goymann, Christel
AU  - Daniels, Rolf
AU  - Savić, Snežana
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1721
AB  - Recently, healthcare professionals again began realizing the benefits of preparing customized medications to meet specific patient needs. The objective of this work was to develop and evaluate simple pharmaceutical bases stabilized with natural-origin surfactant of alkyl polyglucoside (APG) type as prospective ready-to-use bases and compare them to widely used pharmacopoeial ones. Additionally, the ability of the formulated bases to sustain isopropyl alcohol was assessed as well as its influence on ketoprofen skin absorption (as a co-solvent and potential penetration enhancer). In order to evaluate the manifold characteristics a topical drug product should possess, a comprehensive characterization was performed using different techniques. Physicochemical characterization demonstrated satisfactory physical stability of APG-stabilized bases upon the addition of alcohol. In vitro release/permeation studies failed to show significant difference in ketoprofen liberation/permeation profiles from different bases. However, the extent of ketoprofen delivery in vivo was clearly increased from APG bases, relative to that obtained from pharmacopoeia quality one, implying a distinct influence of the emulsion systems' colloidal structures. Taking also into account the rheological behavior of APG bases, revealing their ameliorated sensory characteristics, it could be concluded that the investigated APG bases could be considered as preferential option in drug compounding related to the conventional ones.
PB  - Elsevier Science BV, Amsterdam
T2  - European Journal of Pharmaceutics and Biopharmaceutics
T1  - Compounding of a topical drug with prospective natural surfactant-stabilized pharmaceutical bases: Physicochemical and in vitro/in vivo characterization - A ketoprofen case study
VL  - 80
IS  - 1
SP  - 164
EP  - 175
DO  - 10.1016/j.ejpb.2011.09.001
ER  - 

@article{
author = "Pantelić, Ivana and Lukić, Milica and Malenović, Anđelija and Reichl, Stephan and Hoffmann, Christine and Mueller-Goymann, Christel and Daniels, Rolf and Savić, Snežana",
year = "2012",
abstract = "Recently, healthcare professionals again began realizing the benefits of preparing customized medications to meet specific patient needs. The objective of this work was to develop and evaluate simple pharmaceutical bases stabilized with natural-origin surfactant of alkyl polyglucoside (APG) type as prospective ready-to-use bases and compare them to widely used pharmacopoeial ones. Additionally, the ability of the formulated bases to sustain isopropyl alcohol was assessed as well as its influence on ketoprofen skin absorption (as a co-solvent and potential penetration enhancer). In order to evaluate the manifold characteristics a topical drug product should possess, a comprehensive characterization was performed using different techniques. Physicochemical characterization demonstrated satisfactory physical stability of APG-stabilized bases upon the addition of alcohol. In vitro release/permeation studies failed to show significant difference in ketoprofen liberation/permeation profiles from different bases. However, the extent of ketoprofen delivery in vivo was clearly increased from APG bases, relative to that obtained from pharmacopoeia quality one, implying a distinct influence of the emulsion systems' colloidal structures. Taking also into account the rheological behavior of APG bases, revealing their ameliorated sensory characteristics, it could be concluded that the investigated APG bases could be considered as preferential option in drug compounding related to the conventional ones.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "European Journal of Pharmaceutics and Biopharmaceutics",
title = "Compounding of a topical drug with prospective natural surfactant-stabilized pharmaceutical bases: Physicochemical and in vitro/in vivo characterization - A ketoprofen case study",
volume = "80",
number = "1",
pages = "164-175",
doi = "10.1016/j.ejpb.2011.09.001"
}

Pantelić, I., Lukić, M., Malenović, A., Reichl, S., Hoffmann, C., Mueller-Goymann, C., Daniels, R.,& Savić, S.. (2012). Compounding of a topical drug with prospective natural surfactant-stabilized pharmaceutical bases: Physicochemical and in vitro/in vivo characterization - A ketoprofen case study. in European Journal of Pharmaceutics and Biopharmaceutics
Elsevier Science BV, Amsterdam., 80(1), 164-175.
https://doi.org/10.1016/j.ejpb.2011.09.001

Pantelić I, Lukić M, Malenović A, Reichl S, Hoffmann C, Mueller-Goymann C, Daniels R, Savić S. Compounding of a topical drug with prospective natural surfactant-stabilized pharmaceutical bases: Physicochemical and in vitro/in vivo characterization - A ketoprofen case study. in European Journal of Pharmaceutics and Biopharmaceutics. 2012;80(1):164-175.
doi:10.1016/j.ejpb.2011.09.001 .

Pantelić, Ivana, Lukić, Milica, Malenović, Anđelija, Reichl, Stephan, Hoffmann, Christine, Mueller-Goymann, Christel, Daniels, Rolf, Savić, Snežana, "Compounding of a topical drug with prospective natural surfactant-stabilized pharmaceutical bases: Physicochemical and in vitro/in vivo characterization - A ketoprofen case study" in European Journal of Pharmaceutics and Biopharmaceutics, 80, no. 1 (2012):164-175,
https://doi.org/10.1016/j.ejpb.2011.09.001 . .

TY  - JOUR
AU  - Čalija, Bojan
AU  - Cekić, Nebojša
AU  - Savić, Snežana
AU  - Krajišnik, Danina
AU  - Daniels, Rolf
AU  - Milić, Jela
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1558
AB  - In the present work we investigated the feasibility of chitosan treated Ca-alginate microparticles for delivery of naproxen in lower parts of GIT and evaluated influence of formulation factors on their physicochemical characteristics and drug release profiles. Investigated factors were drug/polymer ratio, chitosan molecular weight, chitosan concentration in hardening medium, and hardening time. Sixteen microparticle formulations were prepared utilizing 24 full factorial design (each factor was varied at two levels). Microparticles size varied between 262.3 +/- 14.9 and 358.4 +/- 21.7 mu m with slightly deformed spherical shape. Low naproxen solubility and rapid reaction of ionotropic gelation resulted in high encapsulation efficiency (> 75.19%). Under conditions mimicking those in the stomach, after two hours, less than 6.18% of naproxen was released. Significant influence of all investigated factors on drug release rate was observed in simulated small intestinal fluid. Furthermore, experimental design analysis revealed that chitosan molecular weight and its concentration had the most pronounced effect on naproxen release. Release data kinetics indicated predominant influence of a pH-dependent relaxation mechanism on drug release from microparticles.
PB  - Pharmaceutical Soc Korea, Seoul
T2  - Archives of Pharmacal Research
T1  - An investigation of formulation factors affecting feasibility of alginate-chitosan microparticles for oral delivery of naproxen
VL  - 34
IS  - 6
SP  - 919
EP  - 929
DO  - 10.1007/s12272-011-0609-y
ER  - 

@article{
author = "Čalija, Bojan and Cekić, Nebojša and Savić, Snežana and Krajišnik, Danina and Daniels, Rolf and Milić, Jela",
year = "2011",
abstract = "In the present work we investigated the feasibility of chitosan treated Ca-alginate microparticles for delivery of naproxen in lower parts of GIT and evaluated influence of formulation factors on their physicochemical characteristics and drug release profiles. Investigated factors were drug/polymer ratio, chitosan molecular weight, chitosan concentration in hardening medium, and hardening time. Sixteen microparticle formulations were prepared utilizing 24 full factorial design (each factor was varied at two levels). Microparticles size varied between 262.3 +/- 14.9 and 358.4 +/- 21.7 mu m with slightly deformed spherical shape. Low naproxen solubility and rapid reaction of ionotropic gelation resulted in high encapsulation efficiency (> 75.19%). Under conditions mimicking those in the stomach, after two hours, less than 6.18% of naproxen was released. Significant influence of all investigated factors on drug release rate was observed in simulated small intestinal fluid. Furthermore, experimental design analysis revealed that chitosan molecular weight and its concentration had the most pronounced effect on naproxen release. Release data kinetics indicated predominant influence of a pH-dependent relaxation mechanism on drug release from microparticles.",
publisher = "Pharmaceutical Soc Korea, Seoul",
journal = "Archives of Pharmacal Research",
title = "An investigation of formulation factors affecting feasibility of alginate-chitosan microparticles for oral delivery of naproxen",
volume = "34",
number = "6",
pages = "919-929",
doi = "10.1007/s12272-011-0609-y"
}

Čalija, B., Cekić, N., Savić, S., Krajišnik, D., Daniels, R.,& Milić, J.. (2011). An investigation of formulation factors affecting feasibility of alginate-chitosan microparticles for oral delivery of naproxen. in Archives of Pharmacal Research
Pharmaceutical Soc Korea, Seoul., 34(6), 919-929.
https://doi.org/10.1007/s12272-011-0609-y

Čalija B, Cekić N, Savić S, Krajišnik D, Daniels R, Milić J. An investigation of formulation factors affecting feasibility of alginate-chitosan microparticles for oral delivery of naproxen. in Archives of Pharmacal Research. 2011;34(6):919-929.
doi:10.1007/s12272-011-0609-y .

Čalija, Bojan, Cekić, Nebojša, Savić, Snežana, Krajišnik, Danina, Daniels, Rolf, Milić, Jela, "An investigation of formulation factors affecting feasibility of alginate-chitosan microparticles for oral delivery of naproxen" in Archives of Pharmacal Research, 34, no. 6 (2011):919-929,
https://doi.org/10.1007/s12272-011-0609-y . .

TY  - JOUR
AU  - Krajišnik, Danina
AU  - Milojević, Maja
AU  - Malenović, Anđelija
AU  - Daković, Aleksandra
AU  - Ibrić, Svetlana
AU  - Savić, Snežana
AU  - Dondur, Vera
AU  - Matijasević, Srdan
AU  - Radulović, Aleksandra
AU  - Daniels, Rolf
AU  - Milić, Jela
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1364
AB  - Context: In this study an investigation of cationic surfactants-modified natural zeolites as drug formulation excipient was performed. Objective: The aim of this work was to carry out a study of the purified natural zeolitic tuff with high amount of clinoptilolite as a potential carrier for molecules of pharmaceutical interest. Materials and methods: Two cationic surfactants (benzalkonium chloride and hexadecyltrimethylammonium bromide) were used for modification of the zeolitic surface in two levels (equal to and twice as external cation-exchange capacity of the zeolitic tuff). Prepared samples were characterized by Fourier transform infrared spectroscopy, thermogravimetric, high-performance liquid chromatography analysis, and powder flow determination. Different surfactant/zeolite composites were used for additional investigation of three model drugs: diclofenac diethylamine, diclofenac sodium, and ibuprofen by means of adsorption isotherm measurements in aqueous solutions. Results: The modified zeolites with two levels of surfactant coverage within the short activation time were prepared. Determination of flow properties showed that modification of zeolitic surface reflected on powder flow characteristics. Investigation of the model drugs adsorption on the obtained composites revealed that a variation between adsorption levels was influenced by the surfactant type and the amount present at the surface of the composites. Discussion and conclusion: In vitro release profiles of the drugs from the zeolite-surfactant-drug composites revealed that sustained drug release could be attained over a period of 8 hours. The presented results for drug uptake by surfactant-zeolite composites and the afterward drug release demonstrated the potential use of investigated modified natural zeolite as excipients for advanced excipients in drug formulations.
PB  - Informa Healthcare, London
T2  - Drug Development and Industrial Pharmacy
T1  - Cationic surfactants-modified natural zeolites: improvement of the excipients functionality
VL  - 36
IS  - 10
SP  - 1215
EP  - 1224
DO  - 10.3109/03639041003695121
ER  - 

@article{
author = "Krajišnik, Danina and Milojević, Maja and Malenović, Anđelija and Daković, Aleksandra and Ibrić, Svetlana and Savić, Snežana and Dondur, Vera and Matijasević, Srdan and Radulović, Aleksandra and Daniels, Rolf and Milić, Jela",
year = "2010",
abstract = "Context: In this study an investigation of cationic surfactants-modified natural zeolites as drug formulation excipient was performed. Objective: The aim of this work was to carry out a study of the purified natural zeolitic tuff with high amount of clinoptilolite as a potential carrier for molecules of pharmaceutical interest. Materials and methods: Two cationic surfactants (benzalkonium chloride and hexadecyltrimethylammonium bromide) were used for modification of the zeolitic surface in two levels (equal to and twice as external cation-exchange capacity of the zeolitic tuff). Prepared samples were characterized by Fourier transform infrared spectroscopy, thermogravimetric, high-performance liquid chromatography analysis, and powder flow determination. Different surfactant/zeolite composites were used for additional investigation of three model drugs: diclofenac diethylamine, diclofenac sodium, and ibuprofen by means of adsorption isotherm measurements in aqueous solutions. Results: The modified zeolites with two levels of surfactant coverage within the short activation time were prepared. Determination of flow properties showed that modification of zeolitic surface reflected on powder flow characteristics. Investigation of the model drugs adsorption on the obtained composites revealed that a variation between adsorption levels was influenced by the surfactant type and the amount present at the surface of the composites. Discussion and conclusion: In vitro release profiles of the drugs from the zeolite-surfactant-drug composites revealed that sustained drug release could be attained over a period of 8 hours. The presented results for drug uptake by surfactant-zeolite composites and the afterward drug release demonstrated the potential use of investigated modified natural zeolite as excipients for advanced excipients in drug formulations.",
publisher = "Informa Healthcare, London",
journal = "Drug Development and Industrial Pharmacy",
title = "Cationic surfactants-modified natural zeolites: improvement of the excipients functionality",
volume = "36",
number = "10",
pages = "1215-1224",
doi = "10.3109/03639041003695121"
}

Krajišnik, D., Milojević, M., Malenović, A., Daković, A., Ibrić, S., Savić, S., Dondur, V., Matijasević, S., Radulović, A., Daniels, R.,& Milić, J.. (2010). Cationic surfactants-modified natural zeolites: improvement of the excipients functionality. in Drug Development and Industrial Pharmacy
Informa Healthcare, London., 36(10), 1215-1224.
https://doi.org/10.3109/03639041003695121

Krajišnik D, Milojević M, Malenović A, Daković A, Ibrić S, Savić S, Dondur V, Matijasević S, Radulović A, Daniels R, Milić J. Cationic surfactants-modified natural zeolites: improvement of the excipients functionality. in Drug Development and Industrial Pharmacy. 2010;36(10):1215-1224.
doi:10.3109/03639041003695121 .

Krajišnik, Danina, Milojević, Maja, Malenović, Anđelija, Daković, Aleksandra, Ibrić, Svetlana, Savić, Snežana, Dondur, Vera, Matijasević, Srdan, Radulović, Aleksandra, Daniels, Rolf, Milić, Jela, "Cationic surfactants-modified natural zeolites: improvement of the excipients functionality" in Drug Development and Industrial Pharmacy, 36, no. 10 (2010):1215-1224,
https://doi.org/10.3109/03639041003695121 . .

TY  - JOUR
AU  - Cekić, Nebojša
AU  - Milić, Jela
AU  - Savić, Snežana
AU  - Savić, Miroslav
AU  - Jović, Žarko
AU  - Daniels, Rolf
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1261
AB  - Background: The potential for use of chitosan-treated alginate microparticles as a vehicle for oral phenytoin delivery has not been thoroughly exploited. Aim: We studied the influence of preparation procedure and chitosan type on physicochemical properties and release behavior of alginate-chitosan microparticles. Method: The total number of 24 microparticles formulations prepared by varying contents of calcium gelling ions and varying contents and type of chitosan was examined. As an additional variable, two different hardening times (1 and 24 hours) were employed. Possible interactions of components, surface morphology of microparticles as well as release profile of phenytoin were studied. Results: Both series of formulations with regard to hardening times, irrespective of the chitosan type and/or concentration employed appeared to be highly loaded with the model drug (above 90%). The drug release studies showed that the kinetics of phenytoin cannot be straightforwardly predicted based on the molecular weight of chitosan alone. On the other hand, prolonging the hardening time from 1 to 24 hours had significantly improved phenytoin kinetics, and gave rise to a formulation with the liberation half-time of about 2.5 hours. Conclusion: This study showed that the latter formulation is eligible for further modifications aimed at improving the regularity of phenytoin absorption.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Drug Development and Industrial Pharmacy
T1  - Influence of the preparation procedure and chitosan type on physicochemical properties and release behavior of alginate-chitosan microparticles
VL  - 35
IS  - 9
SP  - 1092
EP  - 1102
DO  - 10.1080/03639040902774164
ER  - 

@article{
author = "Cekić, Nebojša and Milić, Jela and Savić, Snežana and Savić, Miroslav and Jović, Žarko and Daniels, Rolf",
year = "2009",
abstract = "Background: The potential for use of chitosan-treated alginate microparticles as a vehicle for oral phenytoin delivery has not been thoroughly exploited. Aim: We studied the influence of preparation procedure and chitosan type on physicochemical properties and release behavior of alginate-chitosan microparticles. Method: The total number of 24 microparticles formulations prepared by varying contents of calcium gelling ions and varying contents and type of chitosan was examined. As an additional variable, two different hardening times (1 and 24 hours) were employed. Possible interactions of components, surface morphology of microparticles as well as release profile of phenytoin were studied. Results: Both series of formulations with regard to hardening times, irrespective of the chitosan type and/or concentration employed appeared to be highly loaded with the model drug (above 90%). The drug release studies showed that the kinetics of phenytoin cannot be straightforwardly predicted based on the molecular weight of chitosan alone. On the other hand, prolonging the hardening time from 1 to 24 hours had significantly improved phenytoin kinetics, and gave rise to a formulation with the liberation half-time of about 2.5 hours. Conclusion: This study showed that the latter formulation is eligible for further modifications aimed at improving the regularity of phenytoin absorption.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Drug Development and Industrial Pharmacy",
title = "Influence of the preparation procedure and chitosan type on physicochemical properties and release behavior of alginate-chitosan microparticles",
volume = "35",
number = "9",
pages = "1092-1102",
doi = "10.1080/03639040902774164"
}

Cekić, N., Milić, J., Savić, S., Savić, M., Jović, Ž.,& Daniels, R.. (2009). Influence of the preparation procedure and chitosan type on physicochemical properties and release behavior of alginate-chitosan microparticles. in Drug Development and Industrial Pharmacy
Taylor & Francis Ltd, Abingdon., 35(9), 1092-1102.
https://doi.org/10.1080/03639040902774164

Cekić N, Milić J, Savić S, Savić M, Jović Ž, Daniels R. Influence of the preparation procedure and chitosan type on physicochemical properties and release behavior of alginate-chitosan microparticles. in Drug Development and Industrial Pharmacy. 2009;35(9):1092-1102.
doi:10.1080/03639040902774164 .

Cekić, Nebojša, Milić, Jela, Savić, Snežana, Savić, Miroslav, Jović, Žarko, Daniels, Rolf, "Influence of the preparation procedure and chitosan type on physicochemical properties and release behavior of alginate-chitosan microparticles" in Drug Development and Industrial Pharmacy, 35, no. 9 (2009):1092-1102,
https://doi.org/10.1080/03639040902774164 . .

TY  - JOUR
AU  - Savić, Snežana
AU  - Tamburić, Slobodanka
AU  - Kovacević, Anđelka
AU  - Daniels, Rolf
AU  - Mueller-Goymann, Christel
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1034
AB  - There is a growing need for research into new skin- and environment-friendly surfactants. The aim of the study was to find out whether a combination of an alkylpolyglucoside natural surfactant with established pharmaceutical excipients could provide a solid pharmaceutical base with satisfied physical stability. The study was carried out in two phases: the first one focused on the colloidal structure of vehicles formulated with oils of different polarity and/or different costabilizer (lipophilic versus hydrophilic) and the second one evaluated vehicles' physical stability. A number of techniques were used (polarization, light, and transmission electron microscopy, pH, conductivity and thermogravimetric measurements, rheological analysis and cyclic temperature stress test). Natural surfactant's interaction with used excipients resulted in the formation of semisolid emulsion systems of different rheological profiles, stabilized predominantly by synergistic effects of lamellar liquid-crystalline (L alpha) and complex lamellar gel (L beta) phases. The type of used oil and costabilizer significantly influenced the colloidal structure of the vehicles, particularly in terms of water distribution mode and initial rheological performance as well as their physical stability. It was recommended that medium polar oils of ester type and lipophilic costabilizers, particularly long chain fatty alcohols, should be used in the formulation of stable alkylpolyglucoside-based topical vehicles.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Dispersion Science and Technology
T1  - Natural Surfactant-Based Emulsion Systems: The Influence of Common Pharmaceutical Excipients on Colloidal Structure and Physical Stability
VL  - 29
IS  - 9
SP  - 1276
EP  - 1287
DO  - 10.1080/01932690701857558
ER  - 

@article{
author = "Savić, Snežana and Tamburić, Slobodanka and Kovacević, Anđelka and Daniels, Rolf and Mueller-Goymann, Christel",
year = "2008",
abstract = "There is a growing need for research into new skin- and environment-friendly surfactants. The aim of the study was to find out whether a combination of an alkylpolyglucoside natural surfactant with established pharmaceutical excipients could provide a solid pharmaceutical base with satisfied physical stability. The study was carried out in two phases: the first one focused on the colloidal structure of vehicles formulated with oils of different polarity and/or different costabilizer (lipophilic versus hydrophilic) and the second one evaluated vehicles' physical stability. A number of techniques were used (polarization, light, and transmission electron microscopy, pH, conductivity and thermogravimetric measurements, rheological analysis and cyclic temperature stress test). Natural surfactant's interaction with used excipients resulted in the formation of semisolid emulsion systems of different rheological profiles, stabilized predominantly by synergistic effects of lamellar liquid-crystalline (L alpha) and complex lamellar gel (L beta) phases. The type of used oil and costabilizer significantly influenced the colloidal structure of the vehicles, particularly in terms of water distribution mode and initial rheological performance as well as their physical stability. It was recommended that medium polar oils of ester type and lipophilic costabilizers, particularly long chain fatty alcohols, should be used in the formulation of stable alkylpolyglucoside-based topical vehicles.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Dispersion Science and Technology",
title = "Natural Surfactant-Based Emulsion Systems: The Influence of Common Pharmaceutical Excipients on Colloidal Structure and Physical Stability",
volume = "29",
number = "9",
pages = "1276-1287",
doi = "10.1080/01932690701857558"
}

Savić, S., Tamburić, S., Kovacević, A., Daniels, R.,& Mueller-Goymann, C.. (2008). Natural Surfactant-Based Emulsion Systems: The Influence of Common Pharmaceutical Excipients on Colloidal Structure and Physical Stability. in Journal of Dispersion Science and Technology
Taylor & Francis Inc, Philadelphia., 29(9), 1276-1287.
https://doi.org/10.1080/01932690701857558

Savić S, Tamburić S, Kovacević A, Daniels R, Mueller-Goymann C. Natural Surfactant-Based Emulsion Systems: The Influence of Common Pharmaceutical Excipients on Colloidal Structure and Physical Stability. in Journal of Dispersion Science and Technology. 2008;29(9):1276-1287.
doi:10.1080/01932690701857558 .

Savić, Snežana, Tamburić, Slobodanka, Kovacević, Anđelka, Daniels, Rolf, Mueller-Goymann, Christel, "Natural Surfactant-Based Emulsion Systems: The Influence of Common Pharmaceutical Excipients on Colloidal Structure and Physical Stability" in Journal of Dispersion Science and Technology, 29, no. 9 (2008):1276-1287,
https://doi.org/10.1080/01932690701857558 . .

TY  - JOUR
AU  - Savić, Snežana
AU  - Vuleta, G
AU  - Daniels, Rolf
AU  - Mueller-Goymann, Christel
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/625
AB  - The aim of this study was to examine the lyotropic potential of an alkylpolyglucoside mixed emulsifier (Cetearyl glucoside&Cetearyl alcohol), which belongs to the new generation of natural (sugar) surfactants, and to elaborate the potential stabilization mechanism and relation between the colloid microstructure and water distribution within the systems. Polarization and ordinary light as well as transmission electron microscopy, wide and small-angle X-ray diffraction, thermal analysis and rheological measurement were employed for the systems characterization. It was suggested that Cetearyl glucoside&Cetearyl alcohol stabilizes the o/w creams by synergistic effects of viscoelastic hydrophilic gel of lamellar type and lipophilic gel network built up from cetostearyl alcohol semi-hydrates as well as by lamellar liquid crystalline bilayers surrounding the oil droplets. The hydrophilic gel consists of mixed cetearyl glucoside/cetearyl alcohol crystalline bilayers entrapping the water interlamellarly by hydrogen bonding. It is also showed that oil addition into the chosen binary system influences the creams microstructure significantly, which particularly reflects onto the mode of water distribution within the creams and consequently their potential of skin hydration.
PB  - Springer, New York
T2  - Colloid and Polymer Science
T1  - Colloidal microstructure of binary systems and model creams stabilized with an alkylpolyglucoside non-ionic emulsifier
VL  - 283
IS  - 4
SP  - 439
EP  - 451
DO  - 10.1007/s00396-004-1174-4
ER  - 

@article{
author = "Savić, Snežana and Vuleta, G and Daniels, Rolf and Mueller-Goymann, Christel",
year = "2005",
abstract = "The aim of this study was to examine the lyotropic potential of an alkylpolyglucoside mixed emulsifier (Cetearyl glucoside&Cetearyl alcohol), which belongs to the new generation of natural (sugar) surfactants, and to elaborate the potential stabilization mechanism and relation between the colloid microstructure and water distribution within the systems. Polarization and ordinary light as well as transmission electron microscopy, wide and small-angle X-ray diffraction, thermal analysis and rheological measurement were employed for the systems characterization. It was suggested that Cetearyl glucoside&Cetearyl alcohol stabilizes the o/w creams by synergistic effects of viscoelastic hydrophilic gel of lamellar type and lipophilic gel network built up from cetostearyl alcohol semi-hydrates as well as by lamellar liquid crystalline bilayers surrounding the oil droplets. The hydrophilic gel consists of mixed cetearyl glucoside/cetearyl alcohol crystalline bilayers entrapping the water interlamellarly by hydrogen bonding. It is also showed that oil addition into the chosen binary system influences the creams microstructure significantly, which particularly reflects onto the mode of water distribution within the creams and consequently their potential of skin hydration.",
publisher = "Springer, New York",
journal = "Colloid and Polymer Science",
title = "Colloidal microstructure of binary systems and model creams stabilized with an alkylpolyglucoside non-ionic emulsifier",
volume = "283",
number = "4",
pages = "439-451",
doi = "10.1007/s00396-004-1174-4"
}

Savić, S., Vuleta, G., Daniels, R.,& Mueller-Goymann, C.. (2005). Colloidal microstructure of binary systems and model creams stabilized with an alkylpolyglucoside non-ionic emulsifier. in Colloid and Polymer Science
Springer, New York., 283(4), 439-451.
https://doi.org/10.1007/s00396-004-1174-4

Savić S, Vuleta G, Daniels R, Mueller-Goymann C. Colloidal microstructure of binary systems and model creams stabilized with an alkylpolyglucoside non-ionic emulsifier. in Colloid and Polymer Science. 2005;283(4):439-451.
doi:10.1007/s00396-004-1174-4 .

Savić, Snežana, Vuleta, G, Daniels, Rolf, Mueller-Goymann, Christel, "Colloidal microstructure of binary systems and model creams stabilized with an alkylpolyglucoside non-ionic emulsifier" in Colloid and Polymer Science, 283, no. 4 (2005):439-451,
https://doi.org/10.1007/s00396-004-1174-4 . .