TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2411
AB  - Catechol groups can be involved in redox cycling accompanied by generation of reactive oxygen species (ROS) which may lead to oxidative damage of cellular macromolecules including DNA. The objective of this investigation was to evaluate possible genotoxic effects of a natural catecholamine adrenaline in cultured human lymphocytes using cytogenetic (sister chromatid exchange and micronuclei) and the single cell gel electrophoresis (Comet) assay. In cytogenetic tests, six experimental concentrations of adrenaline were used in a range from 0.01-500 mu M. There were no indications of genotoxic effects of adrenaline in sister chromatid exchange and micronucleus tests. However, at four highest concentrations of adrenaline (5 mu M, 50 mu M, 150 mu M and 300 mu M) we observed a decreased mitotic index and cell-cycle delay. In addition, in the Comet assay we used adrenaline in a range from 0.0005-500 mu M, at two treatment times: 15 min or 60 min. In contrast to cytogenetic analysis, there was a dose-dependent increase of DNA damage detected in the Comet assay. These effects were significantly reduced by concomitant treatment with quercetin or catalase. Therefore, the obtained results indicate that adrenaline may exhibit genotoxic effects in cultured human lymphocytes, most likely due to production of reactive oxygen species.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Toxicology Letters
T1  - Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro
VL  - 29
IS  - 1
SP  - 27
EP  - 33
DO  - 10.1016/j.tiv.2014.08.001
ER  - 

@article{
author = "Đelić, Ninoslav and Radaković, Milena and Potparević, Biljana and Živković, Lada and Bajić, Vladan and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2015",
abstract = "Catechol groups can be involved in redox cycling accompanied by generation of reactive oxygen species (ROS) which may lead to oxidative damage of cellular macromolecules including DNA. The objective of this investigation was to evaluate possible genotoxic effects of a natural catecholamine adrenaline in cultured human lymphocytes using cytogenetic (sister chromatid exchange and micronuclei) and the single cell gel electrophoresis (Comet) assay. In cytogenetic tests, six experimental concentrations of adrenaline were used in a range from 0.01-500 mu M. There were no indications of genotoxic effects of adrenaline in sister chromatid exchange and micronucleus tests. However, at four highest concentrations of adrenaline (5 mu M, 50 mu M, 150 mu M and 300 mu M) we observed a decreased mitotic index and cell-cycle delay. In addition, in the Comet assay we used adrenaline in a range from 0.0005-500 mu M, at two treatment times: 15 min or 60 min. In contrast to cytogenetic analysis, there was a dose-dependent increase of DNA damage detected in the Comet assay. These effects were significantly reduced by concomitant treatment with quercetin or catalase. Therefore, the obtained results indicate that adrenaline may exhibit genotoxic effects in cultured human lymphocytes, most likely due to production of reactive oxygen species.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Toxicology Letters",
title = "Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro",
volume = "29",
number = "1",
pages = "27-33",
doi = "10.1016/j.tiv.2014.08.001"
}

Đelić, N., Radaković, M., Potparević, B., Živković, L., Bajić, V., Stevanović, J.,& Stanimirović, Z.. (2015). Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro. in Toxicology Letters
Pergamon-Elsevier Science Ltd, Oxford., 29(1), 27-33.
https://doi.org/10.1016/j.tiv.2014.08.001

Đelić N, Radaković M, Potparević B, Živković L, Bajić V, Stevanović J, Stanimirović Z. Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro. in Toxicology Letters. 2015;29(1):27-33.
doi:10.1016/j.tiv.2014.08.001 .

Đelić, Ninoslav, Radaković, Milena, Potparević, Biljana, Živković, Lada, Bajić, Vladan, Stevanović, Jevrosima, Stanimirović, Zoran, "Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro" in Toxicology Letters, 29, no. 1 (2015):27-33,
https://doi.org/10.1016/j.tiv.2014.08.001 . .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Milićević, Zorka
AU  - Živković, Lada
AU  - Potparević, Biljana
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1433
AB  - Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono and/or combinational therapies for cancer treatment.
AB  - Prevremena centromerna deoba (PCD) se može posmatrati kao manifestacija hromozomske nestabilnosti. U cilju procene efekta paklitaksela (Ptx) i cikloheksimida (Cy) na indukciju PCD-a, koristili smo mikronukleus test (CBMN) uz fluorescentnu in situ hibridizaciju (FISH), kao i test hromozomskih aberacija (CA) na humanim limfocitima periferne krvi. Rezultati su pokazali da Ptx sam, ili u kombinaciji sa Cy može da indukuje PCD. Ovi nalazi ukazuju da treba mnogo više pažnje obratiti na pojavu PCD-a kao parametra genotoksičnosti u prekliničkim ispitivanjima mono- i/ili politerapija za lečenje kancera.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro
T1  - Efekat samog paklitaksela, i u kombinaciji sa cikloheksimidom, na učestalost prevremene centromerne deobe in vitro
VL  - 62
IS  - 1
SP  - 63
EP  - 74
DO  - 10.2298/ABS1001063B
ER  - 

@article{
author = "Bajić, Vladan and Stanimirović, Zoran and Stevanović, Jevrosima and Milićević, Zorka and Živković, Lada and Potparević, Biljana",
year = "2010",
abstract = "Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono and/or combinational therapies for cancer treatment., Prevremena centromerna deoba (PCD) se može posmatrati kao manifestacija hromozomske nestabilnosti. U cilju procene efekta paklitaksela (Ptx) i cikloheksimida (Cy) na indukciju PCD-a, koristili smo mikronukleus test (CBMN) uz fluorescentnu in situ hibridizaciju (FISH), kao i test hromozomskih aberacija (CA) na humanim limfocitima periferne krvi. Rezultati su pokazali da Ptx sam, ili u kombinaciji sa Cy može da indukuje PCD. Ovi nalazi ukazuju da treba mnogo više pažnje obratiti na pojavu PCD-a kao parametra genotoksičnosti u prekliničkim ispitivanjima mono- i/ili politerapija za lečenje kancera.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro, Efekat samog paklitaksela, i u kombinaciji sa cikloheksimidom, na učestalost prevremene centromerne deobe in vitro",
volume = "62",
number = "1",
pages = "63-74",
doi = "10.2298/ABS1001063B"
}

Bajić, V., Stanimirović, Z., Stevanović, J., Milićević, Z., Živković, L.,& Potparević, B.. (2010). The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(1), 63-74.
https://doi.org/10.2298/ABS1001063B

Bajić V, Stanimirović Z, Stevanović J, Milićević Z, Živković L, Potparević B. The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro. in Archives of Biological Sciences. 2010;62(1):63-74.
doi:10.2298/ABS1001063B .

Bajić, Vladan, Stanimirović, Zoran, Stevanović, Jevrosima, Milićević, Zorka, Živković, Lada, Potparević, Biljana, "The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro" in Archives of Biological Sciences, 62, no. 1 (2010):63-74,
https://doi.org/10.2298/ABS1001063B . .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Milicević, Z.
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1233
AB  - Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3', 5' cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assail (CA) on human peripheral blood lymphocytes of healthy individuals and by hone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 pin) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p) injection of 8-Cl-cAMP in doses of 10, 80 and 15 0 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes
VL  - 14
IS  - 1
SP  - 71
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3670
ER  - 

@article{
author = "Bajić, Vladan and Stanimirović, Zoran and Stevanović, Jevrosima and Potparević, Biljana and Živković, Lada and Milicević, Z.",
year = "2009",
abstract = "Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3', 5' cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assail (CA) on human peripheral blood lymphocytes of healthy individuals and by hone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 pin) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p) injection of 8-Cl-cAMP in doses of 10, 80 and 15 0 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes",
volume = "14",
number = "1",
pages = "71-77",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3670"
}

Bajić, V., Stanimirović, Z., Stevanović, J., Potparević, B., Živković, L.,& Milicević, Z.. (2009). Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 14(1), 71-77.
https://hdl.handle.net/21.15107/rcub_vinar_3670

Bajić V, Stanimirović Z, Stevanović J, Potparević B, Živković L, Milicević Z. Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON. 2009;14(1):71-77.
https://hdl.handle.net/21.15107/rcub_vinar_3670 .

Bajić, Vladan, Stanimirović, Zoran, Stevanović, Jevrosima, Potparević, Biljana, Živković, Lada, Milicević, Z., "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes" in Journal of BUON, 14, no. 1 (2009):71-77,
https://hdl.handle.net/21.15107/rcub_vinar_3670 .